Microrna rna small interfering. Medical search. Frequent questions

Here we explore combinations of different classes of therapeutic anti-HIV-1 RNAs expressed from within the context of an intronic MCM7 (minichromosome maintenance complex component-7) platform that naturally harbors 3 microRNAs (miRNAs). (ca.gov)
siRNA is also similar to miRNA, however, miRNAs are derived from shorter stemloop RNA products. (wikipedia.org)
Circulating microRNAs (miRNAs) are a potential choice for novel biomarkers in AMI diagnosis and prognosis with high sensitivity and specificity. (hindawi.com)
Circulating microRNAs are endogenous miRNAs that are detectable in whole blood, serum, or plasma in a highly stable form. (hindawi.com)
MicroRNAs (miRNAs) seem to be a promising candidate of novel biomarker for early diagnosis of AMI [ 3 ]. (hindawi.com)
miRNAs are endogenous, small (~22 nucleotides), single-stranded, noncoding RNAs that regulate gene expression at the posttranscriptional level by binding to the 3′ untranslated regions (UTRs) of their target mRNAs [ 10 ]. (hindawi.com)
Still other RNA molecules, such as tiny microRNAs ( miRNAs ), act as regulators of other genes, and new types of non-protein-coding RNAs are being discovered all the time. (khanacademy.org)
Small RNAs such as microRNAs (miRNAs) and small interfering (si)RNAs are short 20-24-nucleotide non-coding RNAs. (ncbs.res.in)
Several 22-nucleotide long miRNAs trigger biogenesis of cascades of trans-acting secondary small interfering RNAs, involved in various developmental and stress responses. (ncbs.res.in)
The first class of small RNAs identified were the micro RNAs (miRNAs), which are small (~22 nt) non-coding RNAs that regulate gene expression by base pairing to mRNAs where they direct either mRNA cleavage or repress translation [ 8 ]. (biomedcentral.com)
The test uses nanoparticles to capture tumor-specific microRNAs (miRNAs) in human serum. (mordorintelligence.com)
MicroRNAs (miRNAs) are short (21-24 nucleotides) sequences of RNA that reduce gene expression by promoting the breakdown of messenger RNAs (mRNAs) and by repressing translation of mRNAs into proteins. (freethoughtblogs.com)
MicroRNAs (miRNAs, miRs) are a class of small, non-coding, endogenous and functional RNAs. (biomedcentral.com)
MicroRNAs (miRNAs) are short (about 21 nucleotides long) strands of non-coding RNA that regulate the expression of multiple genes [ 1 ]. (biomedcentral.com)
The two main substrates are hairpin RNAswhich are cut into microRNAs (miRNAs)and long duplex RNAswhich are cut into small interfering RNAs (siRNAs). (berkeley.edu)
Since the discovery in 1993 of the first small silencing RNA, a dizzying number of small RNAs have been identified, including microRNAs (miRNAs), small interfering RNAs (siRNAs) and Piwi-interacting RNAs (piRNAs). (umassmed.edu)
Since complex regulation of the EGFR signaling axis might confound current attempts to inhibit EGFR directly, we searched for microRNAs (miRNAs) that may target the EGFR signaling axis. (oncotarget.com)

In this current study, we utilized an endogenous microRNA (miRNA) cluster as a platform to express multiple anti-HIV small RNAs. (ca.gov)
We demonstrate the versatility of the MCM7 platform in expressing and efficiently processing the siRNAs as miRNA mimics along with nucleolar small RNAs. (ca.gov)
Small interfering RNA (siRNA), sometimes known as short interfering RNA or silencing RNA, is a class of double-stranded RNA at first non-coding RNA molecules, typically 20-24 (normally 21) base pairs in length, similar to miRNA, and operating within the RNA interference (RNAi) pathway. (wikipedia.org)
Examples of these RNA therapies include antisense oligonucleotide (ASO), small interfering RNA (siRNA), and microRNA (miRNA) therapies. (medlineplus.gov)
These small RNAs, which include microRNA (miRNA) and several classes of small interfering RNAs (siRNA), play important roles in regulating gene expression, heterochromatin formation, and defense against invading viruses. (ttu.edu)
The characterization of new miRNA target genes and a unique profile for crasiRNAs has allowed for insight into multiple RNA mediated processes in the tammar, including gene regulation, species incompatibilities, centromere and chromosome function. (biomedcentral.com)
There are also RNAs that are complimentary to mRNA and a gene's downregulation (DN), two of which are MicroRNA (miRNA) and small interfering RNA (siRNA). (differencebetween.net)
1.miRNA is micro ribonucleic acid while siRNA is small interfering ribonucleic acid. (differencebetween.net)
4.Both miRNA and siRNA act on RNA interference (RNAi), but siRNA which is double stranded is best utilized for the cleaving of RNA when incorporated with RNA induced silencing complex (RISC). (differencebetween.net)
Here, we provide a comprehensive global analysis of the evolutionarily distant unicellular green alga Chlamydomonas reinhardtii to quantify the effects of miRNA on protein synthesis and RNA abundance. (freethoughtblogs.com)
These kits are able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time. (norgenbiotek.com)

Finally, anti-HIV RNA decoys that aim to titrate out early essential viral replication proteins Tat and Rev are constructed. (ca.gov)
An RNA therapy called RNA aptamer therapy introduces small pieces of RNA that attach directly to proteins to alter their function. (medlineplus.gov)
This type of RNA is called a messenger RNA ( mRNA ), as it serves as a messenger between DNA and the ribosomes, molecular machines that read mRNA sequences and use them to build proteins. (khanacademy.org)
Further, small interfering RNA (siRNA) against the target gene was co-transfected with miR-149-5p inhibitor, and then the cell apoptosis and the expression of apoptosis-related proteins were assessed. (medscimonit.com)
There are several types of RNA with different functions: messenger RNA (mRNA) which carries information to the protein factories of the cell, transfer RNA (tRNA) which transfers amino acids to the protein synthesis site, ribosomal RNA (rRNA) which catalyzes ribosomes, and transfer messenger RNA (tmRNA) which tags proteins encoded by mRNA. (differencebetween.net)
Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. (norgenbiotek.com)
His fields of research comprise RNA-mediated gene silencing processes with a focus on epigenetic phenomena, including studies on RNA-directed DNA methylation, the characterization of virus silencing suppressor proteins, the development of plant bioreactor platforms and viroid research. (degruyter.com)
Viroids are non-encapsidated, single-stranded (ss), 250-400 nucleotide (nt)-long circular RNA molecules that do not encode proteins (1, 2). (degruyter.com)

One class of such RNA agents is small interfering RNAs (siRNAs) that mediate viral message degradation with perfect base pairing to the target. (ca.gov)
Moreover, combinations with small RNAs capable of turning over multiple target substrates, such as siRNAs and ribozymes, showed greater inhibition to HIV replication in a one-month challenge assay. (ca.gov)
This represents the first efficacious example of combining Drosha-processed siRNAs with small nucleolar ribonucleoprotein (snoRNP)-processed nucleolar RNA chimeras from a single intron platform for effective inhibition of viral replication. (ca.gov)
Naturally occurring siRNAs have a well-defined structure that is a short (usually 20 to 24-bp) double-stranded RNA (dsRNA) with phosphorylated 5' ends and hydroxylated 3' ends with two overhanging nucleotides. (wikipedia.org)
The Dicer enzyme catalyzes production of siRNAs from long dsRNAs and small hairpin RNAs. (wikipedia.org)
Historically, siRNAs were believed to arise only from exogenous double-stranded RNA triggers in organisms lacking RNA-dependent RNA polymerases. (umassmed.edu)
24-28nt small RNAs share many features with piRNAs present in the germline, and a significant fraction of the 24-28nt small RNAs originate from similar transposon clusters as somatic endo-siRNAs. (umassmed.edu)

It acts through RNA interference (RNAi) wherein its effector complex together with enzymes break down mRNA and silence gene activity, block its translation in both plants and animals, and hasten its deterioration. (differencebetween.net)
siRNA, on the other hand, is either a natural or a synthetic and double-stranded molecule that has 22 to 23 nucleotides which also act through RNA interference (RNAi). (differencebetween.net)
RNA interference-mediated YBX1 knockdown significantly reduced cell growth, migration, and invasion. (uzh.ch)
Moreover, microRNA-181 expression was inhibited upon an inactivation of Wnt/β-catenin signaling by an induction of adenomatosis polyposis coli (APC) expression or silencing β-catenin via RNA interference. (biomedcentral.com)
My primary work revolves around a eukaryotic pathway known as RNA interference (RNAi) where small RNA molecules regulate gene expression. (berkeley.edu)
Since then, he has been working as a postodoc in Dr. Wassenegger's lab (RLP AgroScience GmbH, AlPlanta) and his main field of interest is RNA-interference (RNAi) and RNA-directed DNA methylation (RdDM) in plants. (degruyter.com)
Since then, she has been working as a postodoc in Dr. Wassenegger's lab (RLP AgroScience GmbH, AlPlanta) and her main field of interest is RNA-interference (RNAi) and viroid biology in plants. (degruyter.com)

Abstract Combinational therapy with small RNA inhibitory agents against multiple viral targets allows efficient inhibition of viral production by controlling gene expression at critical time points. (ca.gov)
Ribonucleic acid (RNA) which is an important factor in restricting gene expression, mobilizing biological reactions, and in perceiving and transmitting of cellular signals. (differencebetween.net)
Although they are structurally similar, plant and animal microRNAs repress gene expression through very different mechanisms. (freethoughtblogs.com)
The discovery of microRNAs that regulate gene expression has changed our view of cellular biochemistry. (spectrumnews.org)
Chromosomal duplications or deletions may influence autism-linked genes indirectly, by altering fragments of RNA that regulate gene expression, according to a study published 25 February in PLoS One. (spectrumnews.org)
MicroRNAs is a non-coded double stranded RNAs made of approximately of 20 nucleotides disclosed in 1993 by Ambros and Ruvkun laboratories that has significant role in regulation of gene expression at post transcriptional level [1-2]. (pharmafocusasia.com)
This RNA influences gene expression, and so alters cellular operations. (ishinobu.com)
Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. (bvsalud.org)

It interferes with the expression of specific genes with complementary nucleotide sequences by degrading mRNA after transcription, preventing translation. (wikipedia.org)
In many of these techniques, the pieces of RNA interact with a molecule called messenger RNA (or mRNA for short). (medlineplus.gov)
Es gibt drei RNA-Typen, die in der Proteinsynthese eine Rolle spielen: Boten-RNA (englisch messenger RNA, mRNA), Transfer-RNA (tRNA) und die ribosomale RNA (rRNA). (jove.com)
Die Boten-RNA (mRNA) ist eine proteinkodierende RNA, die aus Codons besteht. (jove.com)
It has been identified that the non-coding region of mRNA is capable of providing targeting signals in RNA binding protein domain. (pharmafocusasia.com)

this is what enables the molecules to form the RNA-Induced Silencing Complex (RISC). (wikipedia.org)
For instance, some genes specify ribosomal RNAs ( rRNAs ), which serve as structural components of ribosomes, or transfer RNAs ( tRNAs ), cloverleaf-shaped RNA molecules that bring amino acids to the ribosome for protein synthesis. (khanacademy.org)
Small RNAs have proven to be essential regulatory molecules encoded within eukaryotic genomes. (biomedcentral.com)
As the main enzyme responsible for generating these small RNAs, Dicer measures and cleaves a diverse population of RNA molecules into mature fragments primed to control genes. (berkeley.edu)
At least in plants, DNA regions that become de novo methylated can be defined by homologous RNA molecules in a process termed RNA-directed DNA methylation (RdDM). (degruyter.com)
Viroids are pathogenic circular, non-coding, single-stranded RNA molecules. (degruyter.com)
Upon inoculation, mature Pospiviroidae RNA molecules enter the plant cell nucleus. (degruyter.com)
Various techniques have been developed for targeting cancer cells: gene therapy, monoclonal antibodies (MAbs), antibody toxin conjugates, small-molecule inhibitors, antisense molecules, and tumor vaccines. (medscape.com)
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. (bvsalud.org)

The mechanism by which natural siRNA causes gene silencing through repression of translation occurs as follows: Long dsRNA (which can come from hairpin, complementary RNAs, and RNA-dependent RNA polymerases) is cleaved by an endo-ribonuclease called Dicer. (wikipedia.org)
The resulting transcript is a short hairpin RNA (shRNA), which can be processed into a functional siRNA by Dicer in its usual fashion. (wikipedia.org)
Qi X, Bao FS, and Xie Z. (2009) Small RNA Deep Sequencing Reveals Role for Arabidopsis thaliana RNA-Dependent RNA Polymerases in Viral siRNA Biogenesis. (ttu.edu)
2005) microRNA-directed phasing during trans-acting siRNA biogenesis in plants. (ttu.edu)
Therefore the same RNA can potentially act as a precursor for both endo-siRNA and piRNA-like small RNA biogenesis. (umassmed.edu)

DNA and RNA are polymers (in the case of DNA, often very long polymers), and are made up of monomers known as nucleotides . (khanacademy.org)
It is composed of a chain of nucleotides that allows RNA to encode genetic information just like DNA. (differencebetween.net)

Although the science of the epigenetics of human pain management is in its early stages with relatively few studies that have examined epigenetically mediated mechanisms involved in nociception in human subjects, a key aspect of the review will be to highlight the latest insights into epigenetic processes, including DNA methylation, histone modifications and microRNAs, and describe their involvement in the pathophysiology of chronic visceral pain. (frontiersin.org)
2004) Role of Arabidopsis ARGONAUTE4 in RNA-directed DNA methylation triggered by inverted repeats. (ttu.edu)
The recruitment of this machinery results in the production of viroid-derived small RNAs (vd-sRNAs) that mediate RNA degradation and DNA methylation of cognate sequences. (degruyter.com)
Methylation of small RNAs at the 3´ end in the soma, is a consequence of assembly into a mature Argonaute2-RNA induced silencing complex. (umassmed.edu)

2004) Evolution of microRNA genes by inverted duplication of target gene sequences in Arabidopsis thaliana . (ttu.edu)
Although many studies have analyzed Dicers ability to cleave RNA, there are still many unanswered questions about how Dicer selects its RNA substrates, which can lead to large changes into which genes are eventually regulated. (berkeley.edu)
Los ARNs antisentido sintéticos se utilizan para afectar el funcionamiento de genes específicos en la investigación o con fines terapéuticos. (bvsalud.org)
Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes. (bvsalud.org)

Moreover, we demonstrated enrichment/selection for cells expressing levels of the antiviral RNAs that provide optimal inhibition under the selective pressure of HIV. (ca.gov)
Inhibition of the microRNAs was sufficient to induce EMT in a process requiring upregulation of ZEB1 and/or SIP1. (nih.gov)
MicroRNA-181 expression could be directly induced upon an activation of Wnt/β-catenin signaling, which includes Wnt10B overexpression, inhibition of GSK3β signaling by LiCl, or forced expression of β-catenin/Tcf4. (biomedcentral.com)

Long non-coding (lnc)RNAs play important roles in the development, differentiation, proliferation and apoptosis of cells ( 6 , 7 ). (spandidos-publications.com)
Small RNAs play important roles in many aspects of pre- and post-transcriptional gene regulation, epigenetic modifications, chromosome segregation and genome structure. (biomedcentral.com)

Xie Z . (2011) Biogenesis and function of virus-derived small interfering RNAs in plants. (ttu.edu)

Most eukaryotic organisms possess highly conserved RNA silencing machinery that generates 21- ~ 24-nucleotide small RNAs from double stranded RNA precursors. (ttu.edu)

Interestingly, cells with optimal level of small RNA expression to effectively inhibit viral replication were enriched under the selective pressure of HIV, demonstrating the importance of balancing expression and functionality for successful gene therapy applications. (ca.gov)
Interestingly, ZNFX1 antisense RNA 1 (ZFAS1) could abolish its tumor suppressor effect by binding microRNA (miR)-150, while miR-150 could inhibit the invasion of hepatic carcinoma cells by inhibiting zinc finger E-box binding homeobox 1 (ZEB1) and matrix metalloproteinases (MMP)14 and MMP16 ( 10 ). (spandidos-publications.com)
It can also compete with eukaryotic translation initiation factor (eIF)4G and RNA for eIF4A binding and trap eIF4A in an inactive conformation to inhibit translation initiation via its two highly conserved MA3 domains [3] - [6] . (plos.org)
Extremely low levels of protein may inhibit your body's production of testosterone and interfere with beard growth. (healthline.com)
Pentostatin inhibits adenosine deaminase, resulting in deoxyadenosine and deoxyadenosine 5+-triphosphate accumulation that may inhibit DNA or RNA synthesis, causing cell death. (medscape.com)

When it is incorporated into RNA induced silencing complex (RISC), RNase is activated and causes the cleaving of RNA which is essential in the body's defense against viral infections. (differencebetween.net)

Before this information can be used for protein synthesis, however, an RNA copy (transcript) of the gene must first be made. (khanacademy.org)

deoxyribonucleic acid ( DNA ) and ribonucleic acid ( RNA ). (khanacademy.org)

We demonstrated persistent expression of each anti-HIV RNAs in CEM T cell lines that stably integrate the transgene. (ca.gov)
Furthermore, reverse transcription‑quantitative PCR was used to examine the expression levels of circular RNA‑PVT1 (circ‑PVT1), micoRNA‑195‑5p (miR‑195‑5p) and ETS1 in GC tissues and cells. (spandidos-publications.com)
We found that all five members of the microRNA-200 family (miR-200a, miR-200b, miR-200c, miR-141 and miR-429) and miR-205 were markedly downregulated in cells that had undergone EMT in response to transforming growth factor (TGF)-beta or to ectopic expression of the protein tyrosine phosphatase Pez. (nih.gov)
Together, these microRNAs cooperatively regulate expression of the E-cadherin transcriptional repressors ZEB1 (also known as deltaEF1) and SIP1 (also known as ZEB2), factors previously implicated in EMT and tumour metastasis. (nih.gov)
Conversely, ectopic expression of these microRNAs in mesenchymal cells initiated mesenchymal to epithelial transition (MET). (nih.gov)
Consistent with their role in regulating EMT, expression of these microRNAs was found to be lost in invasive breast cancer cell lines with mesenchymal phenotype. (nih.gov)
Xie Z , Khanna K, Ruan S. (2010) Expression of MicroRNAs and Its Regulation in Plants. (ttu.edu)
EpCAM + AFP + HepCSCs have an activated Wnt/β-catenin signaling with a parallel increased expression of all four microRNA-181 family members. (biomedcentral.com)
Using both western blot and quantitative reverse transcriptase-PCR analyses, we found that the expression of all four microRNA-181 family members was positively correlated with β-catenin expression in HCC cell lines. (biomedcentral.com)
The expression patterns of microRNAs vary less by brain region and age in people with autism than in controls. (spectrumnews.org)
A small fragment of RNA may regulate the expression of RORA, a gene implicated in many autism-related pathways, according to a study published 6 February in Scientific Reports. (spectrumnews.org)
To test for such negative regulatory events, we searched for PU.1-controlled microRNAs (miRs) by expression profiling using a PU.1-inducible myeloid progenitor cell line model. (zfin.org)

Endogenous MCM7 MicroRNA Cluster as a Novel Platform to Multiplex Small Interfering and Nucleolar RNAs for Combinational HIV-1 Gene Therapy. (ca.gov)

Long pieces of RNA that do not code for protein have diverse and important roles in the cell and may contribute to autism risk, say Nikolaos Mellios and Mriganka Sur. (spectrumnews.org)

A total 3 short hairpin RNA (sh)‑lncRNA XIST sequences, overexpression vector (oe)‑lncRNA XIST, microRNA (miR)‑320a mimic, miR‑320a inhibitor, PIK3CA inhibitor, and their corresponding controls were transfected in hepatic carcinoma cells. (spandidos-publications.com)
We examined each of these small RNA classes with respect to the newly assembled tammar wallaby genome for gene and repeat features, salient features that define their canonical sequences, and the constitution of both highly conserved and species-specific members. (biomedcentral.com)

The combinations of si/snoRNAs represent a new paradigm for combinatorial RNA-based gene therapy applications. (ca.gov)

Total RNA and DNA were simultaneously isolated from 50 mL of water sample containing 10 7 cfu/mL E.coli using Norgen's Water RNA/DNA Purification Kit and subsequently run on gels for visual analysis. (norgenbiotek.com)
Norgen's FFPE RNA Purification Kits provide a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. (norgenbiotek.com)
High Quality and Yield of Total RNA. (norgenbiotek.com)
Total RNA was isolated from one slice of hamster FFPE kidney section (20 micron thickness) using Norgen's FFPE RNA Purification Kit and a leading competitors kit. (norgenbiotek.com)
As it can be seen, Norgen not only isolated higher yields of total RNA, but the RNA was also of a higher quality as evidenced by the higher RIN values obtained with Norgen's RNA. (norgenbiotek.com)

dblp: P-SAMS: a web site for plant artificial microRNA and synthetic trans -acting small interfering RNA design. (uni-trier.de)

Fifty mL water samples spiked with increasing amounts of E.coli were filtrated through the provided 0.45 µm water filter columns, and RNase-treated DNA was isolated from the filter by following Norgen's Water RNA/DNA Purification Kit manual. (norgenbiotek.com)
The Norgen FFPE RNA Purification Kit 25300 enable our laboratory to access the RNA and transcriptomic information from the archived paraffin blocks. (norgenbiotek.com)
My customer used the [FFPE Purification] kit with microdissection samples (containing about 100 cells) and she obtained very good results with microRNA. (norgenbiotek.com)
Norgen's FFPE RNA Purification Kit isolates FFPE RNA that exceeds both yield and quality of competitors. (norgenbiotek.com)

It forms intrastrand crosslinks, interfering with DNA replication and RNA transcription and translation. (medscape.com)

In eukaryotes, DNA is typically broken up into a number of very long, linear pieces called chromosomes , while in prokaryotes such as bacteria, chromosomes are much smaller and often circular (ring-shaped). (khanacademy.org)

A Heterochromatin-Specific RNA Export Pathway Facilitates piRNA Production. (oeaw.ac.at)
Taken together, our results demonstrate that microRNA-181s are transcriptionally activated by the Wnt/beta-catenin signaling pathway in HCC. (biomedcentral.com)

The RNA is preferentially purified from other cellular components without the use of phenol or chloroform. (norgenbiotek.com)

The nascent RNA binding complex SFiNX licenses piRNA-guided heterochromatin formation. (oeaw.ac.at)

Using small RNA datasets derived from Indian Himalayan accessions, as well as overexpression and knockout lines of miR158, we show that absence of miR158 led to build up of pseudo-PPR derived tertiary small RNAs. (ncbs.res.in)

The ssRNA(+), likely transferred into the nucleolus, is finally processed by a type III RNase and a DNA LIGASE into unit-length circular RNAs (8) ( Figure 1 ). (degruyter.com)

The Medicines Manufacturing Innovation Centre launched a collaboration with AstraZeneca, Exactmer, Novartis, and UK Research and Innovation to develop a scalable, sustainable, and more cost-effective medicines manufacturing process for oligonucleotides: short strands of synthetic DNA or RNA. (mordorintelligence.com)

Members of the Pospiviroidae family replicate in the nucleus through double-stranded RNA intermediates, attracting the host RNA silencing machinery. (degruyter.com)

The kit incorporates unique D-Plex technology for library preparation enabling high performance with ultralow RNA inputs and providing diverse small RNA transcript detection. (mordorintelligence.com)

Research in my laboratory is directed to understand how multiple small RNA pathways operate in plants using genetic, genomic, and biochemical approaches. (ttu.edu)
Plants, therefore, provide a unique system to study the genetic diversification and functional adaptation of small RNA pathways. (ttu.edu)
Xie Z , and Qi X. (2008) Diverse small RNA-directed silencing pathways in plants. (ttu.edu)
2004) Genetic and functional diversification of small RNA pathways in plants. (ttu.edu)
Ultimately, we want to attain insight into the underlying complexity that interconnects these small RNA pathways. (umassmed.edu)
This thesis research provides vital insight into the network of interactions that fine-tune the small RNA pathways. (umassmed.edu)
Understanding the flow of information between the small RNA pathways, a great deal of which has been revealed only in the recent years, will help us comprehend how the pathways compete and collaborate with each other, enabling each other's optimum function. (umassmed.edu)

These data suggest that downregulation of the microRNAs may be an important step in tumour progression. (nih.gov)
This progression from DNA to RNA to protein is called the " central dogma " of molecular biology. (khanacademy.org)

A universal method for the rapid isolation of all known classes of functional silencing small RNAs. (oeaw.ac.at)

Selective compounds have been developed that target either the extracellular ligand-binding region of the EGFR (including a number of monoclonal antibodies [MAbs], immunotoxins, and ligand-binding cytotoxic agents) or the intracellular tyrosine kinase region (including various small-molecule inhibitors). (medscape.com)

These short RNAs participate in a diverse array of cellular processes including gene regulation, chromatin dynamics and genome defense. (biomedcentral.com)

Cells were transfected with a miR-137 inhibitor, miR-137 mimic, and/or YBX1 small interfering RNA, and growth, colony formation, migration and invasion assays were conducted. (uzh.ch)

Typical transcription cassettes use an RNA polymerase III promoter (e.g. (wikipedia.org)
In addition, seven putative β-catenin/Tcf4 binding sites were identified in the promoter region of the microRNA-181a-2 and microRNA-181b-2 transcripts. (biomedcentral.com)

One elusive part of the protein is the helicase domain, which is involved in the processing of specific RNA substrates. (berkeley.edu)
My research aims to examine how the Dicers helicase domain recognizes the RNA substrates to influence cleavage, using a combination of biochemical and structural techniques. (berkeley.edu)

The core components of RNA silencing machinery involve several evolutionarily conserved protein families, including DICER (DCR) or DICER-LIKE (DCL), ARGONAUTE (AGO), and, in some cases, RNA-DEPENDENT RNA POLYMERASE (RDR). (ttu.edu)
By high throughput sequencing we identified Drosophila endosiRNAs as 21 nt small RNAs, bearing a 2´-O-methyl group at their 3´ ends, and depleted in dicer-2 mutants. (umassmed.edu)

Xie Z ., Cheng H. (2017) Interplay and transition between small RNA-directed posttranscriptional and transcriptional gene silencing in plants. (ttu.edu)

Gene therapy approaches are attractive alternatives where the most vulnerable immune cells are modified with antiviral small RNAs with the goal to engineer an HIV-resistant immune system. (ca.gov)
What are CAR T cell therapy, RNA therapy, and other genetic therapies? (medlineplus.gov)
RNA Therapy: Current Status and Future Potential. (medlineplus.gov)

Having one too many copies of MeCP2, the Rett syndrome gene, may block the growth of neuronal branches by interfering with the production of small pieces of RNA, according to a study published 10 March in Developmental Cell. (spectrumnews.org)

Additionally, the effects of transfection with circ‑PVT1 small interfering RNA (si‑circ‑PVT1) on HGC‑27 and AGS cells could be reversed by treatment with miR‑195‑5p inhibitor. (spandidos-publications.com)

Anatomy20

Organisms17

Diseases7

Chemicals and Drugs93

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes87

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science9

Health Care2

MiRNAs17

MiRNA11

Proteins8

SiRNAs7

Interference7

Gene expression8

MRNA5

Molecules9

SiRNA5

Nucleotides2

Methylation4

Genes4

Inhibition3

Play important roles2

Biogenesis1

Nucleotide1

Inhibit5

Silencing Complex1

Protein synthesis1

Ribonucleic acid1

Expression12

Nucleolar1

Roles1

Sequences2

SnoRNAs1

Total RNA5

Trans -acti1

Purification Kit4

Replication1

Eukaryotes1

Pathway2

Phenol1

Complex1

Overexpression1

RNase1

Synthetic1

Double stranded1

Transcript1

Pathways7

Progression2

Functional1

Molecule1

Processes1

Mimic1

Promoter2

Substrates2

Dicer2

Posttranscriptional1

Therapy3

Developmental1

Transfection1