Mice bone marrow cells in vitro granulocyte macrophage progenitor cells. Medical search. Frequent questions

CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. (thermofisher.com)
CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. (thermofisher.com)
Although tumor cells were first thought to drive the cellular events underpinning tumor angiogenesis and growth, considerable evidence has now emerged for the central role of tumor infiltrating myeloid cells such as monocytes, macrophages, and neutrophils in this phenomenon [ 8 - 12 ]. (hindawi.com)
Phagocytosis of malarial pigment hemozoin (Hz) by monocytes, macrophages, and neutrophils is a central factor for promoting dysregulation in innate inflammatory mediators. (ijbs.com)
However, these immunotherapeutic approaches are hampered by the tumor-promoting nature of myeloid cells, including monocytes, macrophages, and neutrophils. (frontiersin.org)
Myeloid innate immune cells, such as granulocytes, monocytes, macrophages and dendritic cells (DCs), play an important role in cancer-cell recognition, initiation of inflammation and antitumor responses ( 2 ). (frontiersin.org)
Histiocytoses encompass a group of diverse proliferative disorders characterized by the accumulation and infiltration of variable numbers of monocytes, macrophages, and dendritic cells in the affected tissues. (medscape.com)
Macrophages and monocytes are members of the mononuclear phagocyte system. (medscape.com)
Large-scale immunomagnetic selection of CD14+ monocytes to generate dendritic cells for cancer immunotherapy: a phase I study. (nwbio.com)
Ms4a3 is a gene specifically expressed by granulocyte-macrophage progenitors (GMPs), which differentiate into both granulocytes, including neutrophils, and monocytes. (uiowa.edu)
Using Ms4a3-Cre mice crossed to a TdTomato reporter line, neutrophils and monocytes are labeled while lung-resident macrophages, along with lymphocytes and dendritic cells, are not. (uiowa.edu)
Using these mice, we assessed the extent to which GMP-derived inflammatory monocytes and neutrophils contribute to lung infiltration during infection with mouse-adapted SARS-CoV-2. (uiowa.edu)
CD11a is a leukocyte marker that is expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils and eosinophils. (thermofisher.com)
CD11c is expressed in monocytes, macrophages, natural killer cells, some granulocytes and less so in a subset of lymphocytes. (thermofisher.com)
Ly6G is expressed differentially during development by cells in the myeloid lineage including monocytes, macrophages, granulocytes, and neutrophils. (bioxcell.com)
Monocytes typically express Ly6G transiently during development while mature granulocytes and peripheral neutrophils retain expression making Ly6G a good cell surface marker for these populations. (bioxcell.com)

Vaccination with tumor lysate-pulsed dendritic cells elicits antigen-specific, cytotoxic T-cells in patients with malignant glioma. (nwbio.com)
Immature dendritic cells phagocytose apoptotic cells via alphavbeta5 and CD36, and cross-present antigens to cytotoxic T lymphocytes. (nwbio.com)
Dendritic cells acquire antigen from apoptotic cells and induce class I-restricted CTLs. (nwbio.com)
Phase I/II study of vaccination with electrofused allogeneic dendritic cells/autologous tumor-derived cells in patients with stage IV renal cell carcinoma. (nwbio.com)
Babatz J, Röllig C, Löbel B, Folprecht G, Haack M, Günther H, Köhne CH, Ehninger G, Schmitz M, Bornhäuser M. Induction of cellular immune responses against carcinoembryonic antigen in patients with metastatic tumors after vaccination with altered peptide ligand-loaded dendritic cells. (nwbio.com)
Banchereau J, Ueno H, Dhodapkar M, Connolly J, Finholt JP, Klechevsky E, Blanck JP, Johnston DA, Palucka AK, Fay J. Immune and clinical outcomes in patients with stage IV melanoma vaccinated with peptide-pulsed dendritic cells derived from CD34+ progenitors and activated with type I interferon. (nwbio.com)
Maturation and trafficking of monocyte-derived dendritic cells in monkeys: implications for dendritic cell-based vaccines. (nwbio.com)
Vaccination of prostatectomized prostate cancer patients in biochemical relapse, with autologous dendritic cells pulsed with recombinant human PSA. (nwbio.com)
Bleumer I, Tiemessen DM, Oosterwijk-Wakka JC, Völler MC, De Weijer K, Mulders PF, Oosterwijk E. Preliminary analysis of patients with progressive renal cell carcinoma vaccinated with CA9-peptide-pulsed mature dendritic cells. (nwbio.com)
CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells. (thermofisher.com)
N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer. (thermofisher.com)
Pluripotent stem cells can also be committed to produce dendritic cells. (medscape.com)
The importance of dendritic cells in presenting antigens to T and B lymphocytes is increasingly recognized. (medscape.com)
Dendritic cells appear to develop in several pathways. (medscape.com)
[ 11 ] Immature dendritic cells respond to GM-CSF (not to macrophage colony-stimulating factor [M-CSF]) and become committed to generating dendritic cells, which are "professional" antigen-presenting cells (APCs). (medscape.com)
[ 13 ] Dendritic cells are also efficient stimulators of B-cell lymphocytes. (medscape.com)
Effective induction of antigen-specific T-cell responses requires interaction between the dendritic cells and T lymphocytes to prime the latter cells for expansion and subsequent immune responses. (medscape.com)
Dendritic cells display a large amount of MHC-peptide complexes at their surface and can increase the expression of costimulatory receptors and migrate to the lymph nodes, spleen, and other lymphoid tissues, where they activate specific T cells. (medscape.com)

In vitro functional clonogenic assays for IL-7 responsive BM-derived B-cell progenitors demonstrated an increase (∼50%) in colony numbers in the transgenic BM. (sunderland.ac.uk)
The participants critically discussed the need for a precise definition of cancer stem cells, the requirement for new markers and more rapid and tractable in vitro and in vivo assays, and the need to develop drug screening strategies to selectively target cancer stem cells to generate therapeutics for this subpopulation of cells that could be resistant to classic treatments while possessing potent tumor-forming capacity. (aacrjournals.org)
The biological activity of automatically isolated CD133 + cells was evaluated and compared to manually isolated CD133 + cells via functional assays as well as immunofluorescence microscopy. (biomedcentral.com)
5 Functional in vitro assays indicate different physiological roles of MSCs related to their heterogeneity and tissue location of origin. (bmj.com)
The effects of DP on the clonal growth of erythroid and granulocyte-macrophage progenitors were investigated using clonogenic assays according to procedures previously tested in our laboratory. (bmj.com)
To evaluate the leukemogenic role of RUNX1-MT in ASXL1 -mutated cells, we co-expressed RUNX1 -MT (R135T) and ASXL1 -MT (R693X) in different cell lines and performed immunoblot, co-immunoprecipitation, gene expression microarray, quantitative RT-PCR, cell proliferation, differentiation, and clonogenic assays for in vitro functional analyses. (biomedcentral.com)
Progenitor cell function may be measured post-modification by in vitro CFU assays. (stemcell.com)

In experimental settings, the ability of human HSCs to reconstitute hematopoiesis is measured by transplantation into genetically immunocompromised mice (e.g. (stemcell.com)
Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
In addition, the regenerative potential of purified stem cells was assessed 3 weeks after transplantation in immunodeficient mice which had been subjected to experimental myocardial infarction. (biomedcentral.com)
Therefore, hematopoietic stem cells mobilized into a subject's peripheral blood by the method of the invention is useful as a source of donor cells in bone marrow transplantation for the treatment of a variety of disorders, including cancer, anemia, autoimmunity and immunodeficiency. (justia.com)
Deletion of only did not influence the creation of either myeloid, T, or B cells (Shape 1B), whereas or deletion led to a significant reduced amount of all 3 lineages six months after transplantation (Shape 1B). (irpa2006europe.com)
We quantified Compact disc45.2+ cells in Compact disc34?Lin?Sca-1+c-Kit+ (Compact disc34?LSK) HSC, multipotent progenitor (Compact disc34+LSK), and granulocyte/macrophage progenitor (GMP) compartments from the BM six months after transplantation. (irpa2006europe.com)
Using knockin mice expressing functionally stunted β 3 integrin, we show that bone marrow transplantation rescues impaired angiogenesis in these mice by normalizing BMDC recruitment. (rupress.org)
The in vivo effect was investigated using the C57BL/6 mouse bone marrow transplantation (BMT) model. (biomedcentral.com)

CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
1 Unique MSC-specific markers have not yet been identified, and MSCs constitute a heterogeneous cell population, including both multipotent (stem) cells and progenitor cells and might even contain pluripotent cell fractions. (bmj.com)
Mesenchymal stem cells (MSCs) are multipotent cells usually isolated from bone marrow, endometrium, adipose tissues, skin, and dental pulp. (techscience.com)
Mesenchymal stem cells (MSCs) are multipotent stromal cells capable of self-renewal and exhibit multilineage differentiation properties. (techscience.com)

It is now known that new blood vessels originate from preexisting vessels by activation, proliferation and migration of endothelial cells through a process named "angiogenesis" [ 4 ]. (hindawi.com)
Specific growth factors, such as vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF), stimulate the proliferation and migration of naturally quiescent endothelial cells, resulting in the formation of new vessel structures during embryonic development and tumor growth [ 7 ]. (hindawi.com)
Vasculogenesis is the coalescence of new blood vessels from individual endothelial cells or progenitor cells. (hindawi.com)
Upon culture in endothelial growth media, these cells expressed endothelial lineage markers, such as CD31, Tie2, and VEGF receptor 2 (VEGFR2), and incorporated into blood vessels in ischemic tissues. (hindawi.com)
These cells were therefore described as bone marrow derived endothelial progenitor cells (EPCs). (hindawi.com)
Subsequent studies described a VEGFR2 and AC133 expressing subpopulation of these CD34 positive circulating cells that could form endothelial colonies in vitro [ 14 , 15 ]. (hindawi.com)
This suggested that EPCs are able to differentiate into endothelial cells and that such cells are incorporated into sites of active angiogenesis including ischemia, tumor angiogenesis, and metastasis in adult organisms [ 16 , 17 ]. (hindawi.com)
In particular, we focus on the therapeutic potential of endothelial progenitor cells as an exciting new option for the treatment of ischaemic diseases. (biomedcentral.com)
We demonstrate that α v β 3 integrin enhances BMDC recruitment and retention at angiogenic sites by mediating cellular adhesion and transmigration of BMDCs through the endothelial monolayer but not their release from the bone niche. (rupress.org)
Relevant information has now been established on solid tumors and their micro-environments, which include immune, endothelial and mesenchymal cells. (fortunejournals.com)
Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)

Cell populations were then mixed to establish continuous hematopoiesis in allogeneic or syngeneic combinations. (johnshopkins.edu)
Virus host range restriction between compartments was maintained in allogeneic reconstituted cultures which showed active hematopoiesis for 16-17 weeks and no significant differences between: (1) virus infected and uninfected groups or (2) groups comprised of stromal cells from one or the other strain. (johnshopkins.edu)
Thus, these data indicate that the prolongation of hematopoiesis in undisturbed long-term marrow cultures by SFFV occurs through an interaction of adherent hematopoietic stem cells with the marrow stroma. (johnshopkins.edu)
Ineffective hematopoiesis in cases of myelodysplastic syndromes - the role of CD34+ stem and progenitor cells. (bsb-bg.eu)
Unlike most other organs, the bone marrow microenvironment is physiologically hypoxic, a pre-requisite for normal bone marrow hematopoiesis. (haematologica.org)
Depending on the degree of bone marrow damage (i.e., suppression), patients who undergo cytoreductive therapy must also receive therapy to reconstitute bone marrow function (hematopoiesis). (justia.com)
Both transforming growth factor beta (TGF beta) and macrophage inflammatory protein 1 alpha (MIP-1 alpha) have been shown to be multifunctional regulators of hematopoiesis that can either inhibit or enhance the growth of hematopoietic progenitor cells (HPC). (ox.ac.uk)
Chromatin immunoprecipitation sequencing Thirty thousand Lin?Sca?c-Kit+ (LSK) cells were set, sonicated, and put through immunoprecipitation while described.12 Mapping and maximum calling had been done using Bowtie2 (Galaxy edition 2.2.6.2) and EaSeq.16 discussion and LEADS TO investigate the role from the KDM4 enzymes in normal hematopoiesis, a string was performed by us of competitive BM transplantations. (irpa2006europe.com)
The ability to genetically manipulate hematopoietic stem and progenitor cells (HSPCs) in particular has significantly advanced our understanding of the mechanisms that regulate hematopoiesis and is contributing to the development of novel cellular therapies. (stemcell.com)

Among these, Siglec-8 is a CD33-related family member selectively expressed on human mast cells and eosinophils, and at low levels on basophils. (mdpi.com)
Since its discovery in 2000, initial in vitro studies have found that the engagement of Siglec-8 with a monoclonal antibody or with selective polyvalent sialoglycan ligands induced the cell death of eosinophils and inhibited mast cell degranulation. (mdpi.com)
Anti-Siglec-8 antibody administration in vivo to humanized and transgenic mice selectively expressing Siglec-8 on mouse eosinophils and mast cells confirmed the in vitro findings, and identified additional anti-inflammatory effects. (mdpi.com)
granulocytes (neutrophils, eosinophils and basophils) are involved in overcoming infection, parasites and tumors. (justia.com)
Although tumor-promoting functions have been attributed to other granulocytes, like eosinophils ( 17 ), basophils ( 18 ) and mast cells ( 19 ), further research is required to fully elucidate their role in cancer, as antitumoral roles have also been described ( 20 , 21 ). (frontiersin.org)
Eosinophils are currently regarded as the effector cells responsible for much of the pathology of asthma. (edu.pk)
When Th2 lymphocytes are activated to produce cytokines, such as IL-5, this increases the production of eosinophils in the bone marrow and promotes release of these cells into the circulation 8 . (edu.pk)

Transcriptional dynamics of human umbilical cord blood T helper cells cultured in absence and presence of cytokines promoting Th1 or Th2 differentiation was studies. (wikipedia.org)
BACKGROUND: Studies have illuminated that long non-coding RNA (lncRNA) influences bone cell differentiation and formation. (bvsalud.org)
PURPOSE: The research was to explore HAGLR's role in the osteogenic differentiation (OD) process of bone marrow mesenchymal stem cells (BMSCs). (bvsalud.org)
The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
Until recently, differentiation was thought to be the primary function of regenerative cells. (vetstem.com)
PSCs are considered to exert their effects exclusively in intramembranous bone formation, since Ctsk -Cre-mediated deletion of Sp7 , an essential transcriptional factor for osteoblast differentiation, impacted intramembranous but not endochondral bone formation 3 . (nature.com)
Furthermore, we demonstrated that these automatically isolated cells bear proliferation and differentiation capacities comparable to manually isolated cells in vitro. (biomedcentral.com)
Recently, several clinical trials showed an increased left ventricular ejection fraction (LVEF) and an improved regional perfusion following intramyocardial application of cluster of differentiation (CD) 133 + stem cells [ 5 - 9 ]. (biomedcentral.com)
Sequential rounds of division and differentiation give rise to an enormous amplification of cell numbers, necessary for the production of mature blood cells. (justia.com)
This process of division and differentiation is subject to regulation at many levels to control cell production. (justia.com)
Our research focuses on developmental pathways that regulate hematopoietic cell growth and differentiation and are disrupted in the course of neoplastic transformation, particularly in leukemias and lymphomas. (stanford.edu)
Enhanced expression of IL-6, IGF-1, TGF-β, and VEGF has also been observed in LC treated BMSCc+ groups, suggested the cardiac differentiation of BMSCc+, and can be utilized in tissue engineering for cardiac cell therapy. (techscience.com)
The expression of RUNX1 mutant in ASXL1 -mutated myeloid cells augmented proliferation, blocked differentiation, and increased self-renewal activity. (biomedcentral.com)
Additionally, edited CD34 + cells can be further cultured in conditions stimulating lineage-specific differentiation by combining StemSpan™ media with expansion supplements designed for the expansion and differentiation of progenitors to erythroid, myeloid, megakaryocyte, natural killer (NK), or T cell lineages. (stemcell.com)
The first phase is the regulation of proliferation and differentiation in the bone marrow, which is consistent and occurring at a low level in the absence of immunological stimulation. (edu.pk)

Ly6G + MDSCs in Lal-/- mice show strong immunosuppression on T cells, which contributes to impaired T cell proliferation and function in vivo. (wikipedia.org)
Relating in vitro to in vivo exposures with physiologically based tissue dosimetry and tissue response models. (cdc.gov)
In this review, we discuss the rationale for MSC-based cell therapy in COPD, the main findings from in vitro and in vivo preclinical COPD model studies, clinical trials in patients with COPD and directions for further research. (bmj.com)
Over-expression of hypoxia-inducible factor in multiple myeloma plasma cells strongly induced in vivo angiogenesis, and administration of a CXCL12 antagonist decreased hypoxia-inducible factor-induced angiogenesis. (haematologica.org)
Regulation of immunomodulatory functions by granulocyte-macrophage colony-stimulating factor and granulocyte colony-stimulating factor in vivo. (nwbio.com)
In vivo, MPL515/630 but not MPL515/625 or MPL515/625/630 induced typical features of MPNs with high WBC and platelet counts, splenomegaly, hepatomegaly and hypercellularity in the bone marrow. (biomedcentral.com)
The existing affiliation for K.D.R. for hematopoietic stem cell (HSC) maintenance in vivo. (irpa2006europe.com)

Aberrantly expressed cytokines in the bone marrow (BM) niche are increasingly recognized as critical mediators of survival and expansion of leukemic stem cells. (haematologica.org)
Out of the 313 unique human cytokines evaluated, 11 were found to expand cell numbers ≥2-fold in a 7-day culture. (haematologica.org)
Cytokines are essential for the function and maintenance of cells, and altered cytokine levels influence not only leukemic cells, but also the normal HSC within the BM. (haematologica.org)
The innate immune response by myeloid cells occurs as a succession of events starting at signaling through cytosolic or surface PRRs, followed by effector responses including the release of cytokines, reactive oxygen species (ROS), reactive nitrogen species (RNS), antibacterial peptides and degranulation ( Figure 1 ). (frontiersin.org)
Pathogen clearance is mediated by mechanisms such as phagocytosis, respiratory burst with the production of ROS and RNS and release of bacteriostatic peptides, but also through the cell-extrinsic initiation of inflammation via the release of proinflammatory cytokines and chemokines ( 24 ). (frontiersin.org)
We report here the spectrum of activities of these two cytokines on different hematopoietic progenitor and stem cell populations, and whether these effects are direct or indirect. (ox.ac.uk)
MERS 8b KO virus showed increased virulence in mice, with significant increases in virus titer and expression levels of inflammatory cytokines, including IFN-a, IFN-b, and ISG15 in the lungs. (uiowa.edu)

CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)
Interferon-gamma-mediated pathways are induced in human CD34(+) haematopoietic stem cells. (bsb-bg.eu)
13 ] isolated mononuclear cells from human peripheral blood that were enriched for expression of the hematopoietic stem cell marker CD34 [ 13 ]. (hindawi.com)
To identify regulators of primitive chronic myeloid leukemia (CML) cells, we performed a high-content cytokine screen using primary CD34 + CD38 low chronic phase CML cells. (haematologica.org)
5 In this study we started with a highly enriched population of CD34+ progenitor cells 6 obtained by incubating the mononuclear fraction of blood and bone marrow with biotinylated anti-CD34 antibodies and running the CD34 labelled cells over an avidin column (Cellpro, Inc., Bothell, Washington, USA). (bmj.com)
This Technical Bulletin provides instructions for CRISPR-Cas9 genome editing and maintenance of human CD34 + cells, including optimized pre- and post-editing culture conditions and methods to evaluate genome editing efficiency. (stemcell.com)
While CRISPR-Cas9 technology has been successfully applied in numerous cell lines, its application in primary human CD34 + cells has been hampered by challenges in efficient expression and delivery of CRISPR-Cas9 components. (stemcell.com)
The ArciTect™ CRISPR-Cas9 RNP is then prepared and delivered into CD34 + cells using electroporation, and cells are plated in StemSpan™ SFEM II supplemented with StemSpan™ CD34 + Expansion Supplement post-electroporation. (stemcell.com)

Furthermore, a subgroup of homozygous Eμ-fHDAC9 mice (n=16) developed tumours (81%) at middle age, and present with enlarged lymph nodes (6%) and abnormal hematopoietic elements in peripheral blood and BM. (sunderland.ac.uk)
Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control Super Bright 645 (Product # 64-4714-82) (blue histogram) or Anti-Human CD14 Super Bright 645 (purple histogram). (thermofisher.com)
Applications Tested: This 61D3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
Influence of cobalt chloride on mouse peripheral blood cells. (bsb-bg.eu)
3 - 5 CXCL12 is highly expressed by MM plasma cells, 6 and circulating levels of CXCL12 are higher in the peripheral blood of MM patients than in age-matched normal donors and MGUS patients. (haematologica.org)
Improved methods and pharmaceutical compositions are provided herein for mobilizing hematopoietic progenitor cells from bone marrow into peripheral blood, comprising the administration of an effective amount of an inhibitor of GTPases, such as Rac1 and Rac2 alone or in combination. (justia.com)
Specifically, methods are disclosed for mobilizing hematopoietic stem cells into a subject's peripheral blood. (justia.com)
In particular, embodiments of the method involve inhibition of both Rac1 and Rac2 GTPases to increase the numbers of hematopoietic stem cells into a subject's peripheral blood of a subject. (justia.com)
The present invention relates generally to improved methods and pharmaceutical compositions for mobilizing hematopoietic stem and progenitor cell from bone marrow into peripheral blood by administration of at least one inhibitor of a GTPase, such as Rac1 and/or Rac2 GTPase. (justia.com)
All experiments performed on red cell lysed murine peripheral blood in the presence of murine SeroBlock ( BUF041A ). (bio-rad-antibodies.com)

Activated γδ T cells exhibit strong cytotoxic activity and cytokine secretion functions and are effective antitumor lymphocytes with simple and direct recognition modes and rapid responses. (explorationpub.com)
The first signal may involve interaction between an MHC I-bound and/or MHC II-bound peptide on an APC with the T-cell receptor (TCRs) on the effector lymphocytes. (medscape.com)

Tumors exhibit significant myeloid cell infiltrates, which are actively recruited to the tumor microenvironment. (hindawi.com)
Finally, we will comment on the mechanisms regulating myeloid cell recruitment to the tumor microenvironment and on the potential of myeloid cells as new targets for cancer therapy. (hindawi.com)
In the tumor microenvironment, tumor-associated macrophages (TAMs) display an array of phenotypes beyond the M1/M2 paradigm, ranging from antitumoral to immunosuppressive, proangiogenic, immunomodulatory and tissue-remodeling phenotypes ( 7 - 9 ). (frontiersin.org)
γδ T cells recruited into the tumor microenvironment can act as effector cells to mediate cancer immune surveillance. (explorationpub.com)
Therefore, an in-depth understanding of γδ T cells that play conflicting roles in the tumor microenvironment is necessary. (explorationpub.com)

MIP-1 alpha enhances interleukin-3 (IL-3)/and granulocyte-macrophage colony-stimulating factor (GM-CSF)/induced colony formation of normal bone marrow progenitor cells (BMC) and lineage-negative (Lin-) progenitors, but has no effect on G-CSF or CSF-1/induced colony formation. (ox.ac.uk)

The innate immune system is mainly composed of myeloid lineage cells, such as macrophages, neutrophils, and mast cells [ 2 ]. (hindawi.com)

We have studied the sensitivities of four hematopoietic stem cell types to heat stress as well as their abilities to develop thermotolerance. (elsevierpure.com)
Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm caused by an acquired 9;22-chromosomal translocation in a hematopoietic stem cell (HSC) resulting in the expression of the BCR-ABL1 fusion protein. (haematologica.org)

Moreover, morphologic and flow cytometric analyses of the transgenic colonies, but not those derived from normal BM, revealed the presence of granulocyte/macrophage colony forming units expressing the HDAC9 transgene, suggesting a lympho-myeloid lineage switch. (sunderland.ac.uk)
Also making up a part of the hematopoietic system are short-term repopulating progenitor cells, which give rise to lineage-specific cell types. (stemcell.com)
The ontogeny and fate of stem cells have been extensively investigated by lineage-tracing approaches. (nature.com)
1 In humans, glucocorticoid receptors have been shown in many different cells, including those of the granulocyte-macrophage lineage and lympho cytes. (bmj.com)

The bone marrow cell suspensions were then heated in vitro and plated for colony formation. (elsevierpure.com)
Concentrations of 10 6 M to 10 9 M were associated with a dose dependent inhibition of colony formation, with the most clinically important effects seen on the earliest erythroid and granulocyte-macrophage colonies. (bmj.com)
Similarly, TGF beta enhances GM-CSF/induced colony formation of normal BMC and Lin- progenitors. (ox.ac.uk)
In contrast, TGF beta inhibits IL-3/ and CSF-1/induced colony formation of Lin- progenitors. (ox.ac.uk)
The effects of MIP-1 alpha and TGF beta on the growth of Lin- progenitors were direct and correlate with colony formation in soft agar. (ox.ac.uk)
Furthermore, MIP-1 alpha has no effect on high proliferative potential colony-forming cells 1 or 2 (HPP-CFC/1 or /2) colony formation in vitro, whereas TGF beta inhibits both HPP-CFC/1 and HPP-CFC/2. (ox.ac.uk)

The four stem cell precursors differed markedly in their abilities to develop thermotolerance. (elsevierpure.com)
A workshop was convened by the AACR to discuss the rapidly emerging cancer stem cell model for tumor development and progression. (aacrjournals.org)
If the cancer stem cell model is correct and if such cells retain the hallmarks of some tissue stem cells in being rare and entering the cell cycle infrequently, they could constitute a population that is intrinsically resistant to current therapies designed to kill cycling cells. (aacrjournals.org)
In the cancer stem cell model of tumors, there is a small subset of cancer cells, the cancer stem cells, which constitute a reservoir of self-sustaining cells with the exclusive ability to self-renew and maintain the tumor. (aacrjournals.org)
These cancer stem cells have the capacity to both divide and expand the cancer stem cell pool and to differentiate into the heterogeneous nontumorigenic cancer cell types that in most cases appear to constitute the bulk of the cancer cells within the tumor. (aacrjournals.org)
Success in numerous animal models of disease and emerging success in human clinical trials for Crohn's fistulas 1 and stroke, 2 along with hundreds of ongoing clinical trials (see sidebar) support the rationale for stem cell use, and now success, in veterinary medicine. (vetstem.com)
3-6 The first peer-reviewed double-blinded multicenter study for adipose-derived stem cell therapy use in canine osteoarthritis of the hip showed significant improvement in all post treatment evaluation times for lameness, pain, and range of motion. (vetstem.com)
We demonstrate here that GSK-3 maintains the MLL leukemia stem cell transcriptional program by promoting the conditional association of CREB and its coactivators TORC and CBP with homedomain protein MEIS1, a critical component of the MLL-subordinate program, which in turn facilitates HOX-mediated transcription and transformation. (stanford.edu)
Distinct and overlapping direct effects of macrophage inflammatory protein-1 alpha and transforming growth factor beta on hematopoietic progenitor/stem cell growth. (ox.ac.uk)
In contrast, two other stem cell populations (0% to 15% Höechst 33342/Rhodamine 123 [Hö/Rh123] and Lin-Sca-1+ cells) were markedly inhibited by TGF beta and unaffected by MIP-1 alpha. (ox.ac.uk)
Different approaches to evaluate the future of biomaterials and stem cell properties have been developed. (techscience.com)
The Mesenchymal and Tissue Stem Cell Committee, which belongs to the International Society for Cellular Therapy, stated that plastic-adherent properties are the minimum criteria for determining MSCs. (techscience.com)

The stromal fraction that is harvested from adipose tissue is a heterogeneous mixture of regenerative cells (see below). (vetstem.com)
At distinct anatomical sites, bone tissue harbors multiple types of skeletal stem cells, which may independently supply osteogenic cells in a site-specific manner. (nature.com)
According to the European Medicines Agency (EMA) BM-derived CD133 + stem cells intended for the regeneration of human ischemic heart tissue are classified as an advanced therapy medicinal product (ATMP) [ 17 ]. (biomedcentral.com)
In vitro cell culture and animal studies have demonstrated that mesenchymal stromal cells (MSCs) have the capacity to modify immune responses and to enhance tissue repair. (bmj.com)
3 4 Since then, it has become evident that MSCs reside in many tissues, including mesenchymal tissues (bone, adipose tissue, connective tissue), umbilical cord and several organs including the liver, spleen and lung. (bmj.com)
Histiocytes are tissue-resident macrophages. (medscape.com)
If the underlying ischaemia is not sufficiently resolved it can lead to tissue damage, with subsequent cell death. (biomedcentral.com)
This review will examine the clinical potential of several stem and progenitor cells that may be utilised to regenerate defunct or damaged vasculature and restore blood flow to the ischaemic tissue. (biomedcentral.com)
This leads to hypoxia and tissue damage as a consequence of the build up of waste metabolites and may result in cell death [ 1 ]. (biomedcentral.com)
Cells for vascular therapy must be able to home to ischaemic or damaged tissue and engage in vessel formation alone or in unison with resident vasculature to achieve a controlled and functional reperfusion event, without causing pathological angiogenesis (for example, proliferative retinopathy in the vitreous of the eye). (biomedcentral.com)
A cell therapy approach should be aimed at promoting revascularisation of ischaemic tissue. (biomedcentral.com)
There is a therapeutic window in which to deliver the cells, to avoid extensive tissue damage, fibrosis and necrosis. (biomedcentral.com)
Study style Animal research Mouse lines and tamoxifen shot procedures have already been referred to.9,11 Bone tissue marrow (BM) and peripheral bloodstream (PB) cells were isolated and stained as referred to.12 All animal research were approved by the Danish Animal Ethical Committee. (irpa2006europe.com)
However, it can be challenging to differentiate when they are connected with wounded tissue, opposed to when they instruct tissue-specific progenitor cells responsible for the redevelopment of damaged tissue. (techscience.com)

METHODS: BMSCs were isolated from mouse bone marrow tissues and identified by electron microscope and flow cytometry. (bvsalud.org)
In vascular medicine, various stem cells and adult progenitors have been highlighted as having a vasoreparative role in ischaemic tissues. (biomedcentral.com)
The evaluation of the most suitable timing of cell delivery as well as the number of cells needed to integrate into resident vasculature and promote revascularisation of specific tissues requires careful optimisation and evaluation. (biomedcentral.com)
hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
Critically, however, how each cell moves and changes shape (that is, behaves) is the result of genetic and signalling networks that vary across tissues and cell types8, and should therefore contain information about core features of the cell. (studylib.net)
Behavioural immune profiles To test this possibility, we used existing10,11 and new imaging experiments in defined tissues and inflammatory contexts, in which myeloid 1 Area of Cell and Developmental Biology, Centro Nacional de Investigaciones Cardiovasculares Carlos III, Madrid, Spain. (studylib.net)

1 The BCR-ABL1 fusion protein is a constitutively active tyrosine kinase and triggers a cascade of aberrant downstream signaling pathways leading to clonal outgrowth of CML cells and subsequent disease manifestation. (haematologica.org)
Multiple myeloma (MM) is an incurable hematologic malignancy characterized by the clonal proliferation of malignant plasma cells in the bone marrow. (haematologica.org)
The in vitro effect of dexamethasone on the clonal growth of haematopoietic progenitors in preterm infants was investigated. (bmj.com)
As a first step toward the goal of defining the effects of DP treatment on haematopoiesis of preterm infants, we designed this in vitro study to determine whether DP affects the clonal growth of erythroid or granulocyte-macrophage progenitor cells in preterm infants, and if the preterm infant's progenitors respond to DP in a different manner from those of adults. (bmj.com)

Additional markers can be used to distinguish HSPC subsets within the CD34+ population and isolate HSPCs with different engraftment abilities and capacities to expand or generate mature blood cells in culture. (stemcell.com)
While our initial manuscript focused on our unexpected finding that committed progenitor subsets could not be identified in mitotic progenitors, all reviewers requested further analysis of when distinct interneuron subsets first emerge during development, and how heterogeneity in the eminences could be connected to later timepoints. (authorea.com)
Separation of the Lin- cells into Thy-1 and Thy-1lo subsets showed that the growth of Thy-1lo Lin- cells is directly inhibited by MIP-1 alpha and TGF beta regardless of the cytokine used to stimulate growth (IL-3), GM-CSF, or CSF-1). (ox.ac.uk)
Single-cell RNA-seq unmasked the existence of two subsets within the LT-HSC population that was apparent upon stimulation but not steady-state. (biorxiv.org)
One of the LT-HSC subsets is more prevalent in young and the other in aged mice. (biorxiv.org)
It is of great significance to gain insight into the classification of γδ T cell subsets. (explorationpub.com)

Myeloid cells promote tumor growth by stimulating tumor angiogenesis, suppressing tumor immunity, and promoting metastasis to distinct sites. (hindawi.com)
In this review, we discuss the role of myeloid cells in promoting tumor angiogenesis. (hindawi.com)
Importantly, during tumor progression, myeloid cells are implicated in promoting tumor angiogenesis, causing resistance against antiangiogenic therapies in cancer, and suppressing the immune response during cancer [ 3 - 5 ]. (hindawi.com)
Progression of multiple myeloma is accompanied by an increase in bone marrow angiogenesis. (haematologica.org)
Studies from our laboratory suggest a role for the CXCL12 chemokine in this process, with circulating levels of CXCL12 correlating with bone marrow angiogenesis in patients with multiple myeloma. (haematologica.org)
Conclusions Hypoxia-inducible factor-2 is a newly identified regulator of CXCL12 expression in multiple myeloma plasma cells and a major contributor to multiple myeloma plasma cell-induced angiogenesis. (haematologica.org)
6 , 7 CXCL12 is an important mediator of several aspects of MM biology including transendothelial migration, 8 , 9 MM plasma cell migration and retention within the bone marrow, 10 , 11 angiogenesis, 7 and osteoclastic bone resorption. (haematologica.org)
14 , 15 HIF-1 and HIF-2 mediate adaptive responses to hypoxia by inducing the transcription of genes associated with erythropoiesis, glycolytic metabolism, cell survival and angiogenesis. (haematologica.org)
Angiogenesis is dependent on the coordinated action of numerous cell types. (rupress.org)
Here, we show that although this receptor is present on most vascular and blood cells, the key regulatory function in tumor and wound angiogenesis is performed by β 3 integrin on bone marrow-derived cells (BMDCs) recruited to sites of neovascularization. (rupress.org)
This chapter provides an overview of the key role of hEPC in promoting angiogenesis and their potential use for cell therapy. (intechopen.com)

Of the erythroid progenitors tested, erythrocyte colony forming units were more resistant than the two more primitive erythrocyte burst forming units. (elsevierpure.com)
For erythroid colony forming unit (CFU-E) quantification, we used 2000 cells/ml, while for erythroid burst forming units (BFU-E), colony forming units mix (CFU-mix), and granulocyte-macrophage colony-forming unit's (CFU-GM), we used 7000 cells/ml. (bmj.com)
The concentrations of erythropoietin (Epo) used to promote the growth of erythroid colonies ranged from 0.2 U/ml to 20 U/ml, based on the possibility that very high Epo concentrations might mask the effects of DP on progenitors of preterm infants. (bmj.com)

These cells can participate in inflammatory responses by releasing mediators that attract or activate other cells, contributing to the pathogenesis of allergic and non-allergic diseases. (mdpi.com)
A pro-inflammatory environment is thought to provide a selective advantage for the leukemic stem cells (LSC). (haematologica.org)
Here, we show that young and aged LT-HSCs respond differently to inflammatory stress, such that aged LT-HSCs produce a cell-intrinsic, myeloid-biased expression program. (biorxiv.org)
Using single-cell RNA-seq, we identify a myeloid-biased subset within the LT-HSC population (mLT-HSCs) that is much more common amongst aged LT-HSCs and is uniquely primed to respond to acute inflammatory challenge. (biorxiv.org)
LT-HSCs from young and aged mice have differential responses to acute inflammatory challenge. (biorxiv.org)
HSPCs directly sense inflammatory stimuli in vitro and have a robust transcriptional response. (biorxiv.org)
Aged LT-HSCs demonstrate a cell-intrinsic myeloid bias during inflammatory challenge. (biorxiv.org)
One sentence summary Murine hematopoietic stem cells display transcriptional heterogeneity that is quantitatively altered with age and leads to the age-dependent myeloid bias evident after inflammatory challenge. (biorxiv.org)
This domain interacts with adaptor molecules and activates a cascade which regulates the inflammatory status in cells [12]. (fortunejournals.com)
Behavioural screening in 24 mouse mutants identified the kinase Fgr as a driver of this pathogenic state, and interference with Fgr protected mice from inflammatory injury. (studylib.net)
Current technologies, however, cannot capture rapid transitions between cellular states in highly dynamic scenarios, such as during acute inflammatory responses, which are characterized by continuous changes in the motility and morphology of individual cells as they adapt to local cues and anatomical barriers. (studylib.net)
Inflammatory cells, most especially neutrophils, can be a necessary component of the antitumor activity occurring after administration of photodynamic therapy. (bioxcell.com)

Colonies derived from different types of progenitor cells are classified and counted based on morphological and phenotypic criteria. (stemcell.com)

Decreased histone acetylation is a hallmark of cancer cells and increased HDAC expression or their mistargetting to specific gene promoters has been associated with a variety of tumors. (sunderland.ac.uk)
Indeed, one critical question contemplated at the Workshop was whether tumors derive from organ stem cells that retain self-renewal properties but acquire epigenetic and genetic changes required for tumorigenicity or whether tumor stem cells are proliferative progenitors that acquire self-renewal capacity. (aacrjournals.org)
If cancer stem cells are relatively refractory to therapies that have been developed to eradicate the rapidly dividing cells within the tumor that constitute the majority of the nonstem cell component of tumors, then they are unlikely to be curative and relapses would be expected. (aacrjournals.org)
Myeloid cells are a heterogeneous population of bone marrow-derived cells that play a critical role during growth and metastasis of malignant tumors. (hindawi.com)
In 1971, Dr. Judah Folkman observed that neovascularization occurs around tumors and proposed that new blood vessel growth is necessary to supply nutrients and oxygen to tumor cells during exponential tumor growth [ 6 ]. (hindawi.com)
Immune cells abundantly infiltrate tumors, creating a complex environment mediated by repetitive cycles of antitumor responses and immune evasion ( 1 ). (frontiersin.org)
In addition to TAMs, solid tumors are also infiltrated by immunosuppressive, immature myeloid progenitor cells, commonly referred to as monocytic or polymorphonuclear myeloid-derived suppressor cells (M/PMN-MDSC) ( 11 - 13 ). (frontiersin.org)
The complexity of the human bone marrow, with context to haematological malignancies, is more difficult to elucidate with the present strategies compared to the niche of solid tumors. (fortunejournals.com)
There can, however, be few similarities between the two, as the bone marrow is a common site of metastasis of solid tumors too [6]. (fortunejournals.com)
2016). "Eradication of large established tumors in mice by combination immunotherapy that engages innate and adaptive immune responses" Nat Med. (bioxcell.com)

Other cell types derived from hematopoietic stem cells include platelets and erythrocytes. (justia.com)

Collectively, these are referred to as hematopoietic stem and progenitor cells (HSPCs). (stemcell.com)
and today is the most widely used in vitro assay for HSPCs. (stemcell.com)

CD43 expression has also been detected on pluripotent hematopoietic stem cells and myeloid, lymphoid, and NK-cell progenitors in the bone marrow. (bdbiosciences.com)
True stem cells are both pluripotent-that is they can give rise to all cell types-and capable of self-renewal. (justia.com)
Normal histiocytes originate from pluripotent stem cells, which can be found in bone marrow. (medscape.com)

The HDAC9 gene is located in chromosome 7p21, which is frequently amplified in B-cell tumours such as mantle cell lymphoma (MCL) and in B-cell non-Hodgkin's lymphoma cell lines. (sunderland.ac.uk)
In order to examine the role of HDAC9 in the lymphoid development and pathogenesis of lymphoid malignancies we used Ig heavy chain enhancer (Eμ), which drives gene expression from early stages of B-cell development, to ectopically express HDAC9 in transgenic mice. (sunderland.ac.uk)
Methods & results: Genetic and related clinical traits of chromophobe renal cell carcinoma were downloaded from the Cancer Genome Atlas and used to construct modules using weighted gene coexpression network analysis. (bvsalud.org)
We are studying the role that normal chromatin structure plays in gene regulation in hematopoietic cells and how its disruption leads to altered development and cancer. (stanford.edu)
Identification of PARD3 signature on PARD3 deficient H157 cell line, reconstituting the expression of PARD3 gene, with a wt and a mutant form. (nih.gov)
This gene is known as ORF9b, ORF8b, and I for Severe Acute Respiratory Syndrome (SARS-CoV-2), Middle East Respiratory Syndrome (MERS-CoV), and Mouse Hepatitis virus (MHV), respectively. (uiowa.edu)
In-vitro infection of bone marrow-derived macrophages with JHM I KO was attenuated at the replication level, but less so at the genomic level, indicating a potential unclear structural or assembly role of the I gene. (uiowa.edu)
Moreover, the RUNX1 mutant protein was more stable than WT and increased HIF1-α and its target ID1 gene expression in ASXL1 mutant cells. (biomedcentral.com)

All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. (bio-rad-antibodies.com)

The entire manufacturing process, as well as the subsequent quality control of the final cell product (CP), were realized on-site and in compliance with EU guidelines for Good Manufacturing Practice. (biomedcentral.com)
The use of high-dosage chemotherapy or radiotherapy for bone marrow ablation requires subsequent incorporation of hematopoietic stem cells into the patient, in which case prior harvesting of such cells is required. (justia.com)
The success of treatment crucially depends on the mobilization of the bone marrow stem cells, the subsequent return of which permits the patient to achieve reconstitution of a functioning hematopoietic system. (justia.com)

Replication of the Friend spleen focus forming virus (SFFV) in C3H/HeJ or C57BL/6J mouse continuous bone marrow cultures is associated with an increased cumulative production of pluripotential/hematopoietic stem cells (CFUs), granulocyte-macrophage progenitor cells (GM-CFUc), and total granulocytes, compared to uninfected or helper virus infected control cultures. (johnshopkins.edu)
however, the drawback of this approach is that this may result in cells localising to non-target organs such as the liver, kidneys, spleen and lung. (biomedcentral.com)
Mice transduced with both ASXL1 and RUNX1 mutations enhanced inhibitor of DNA binding 1 (ID1) expression in the spleen, liver, and bone marrow cells. (biomedcentral.com)

The use of cell therapy for vascular regeneration offers an exciting new prospect in regenerative medicine. (biomedcentral.com)
However, the delivery of the correct cell type to the precise area of injury or vascular insufficiency is difficult and many factors need to be considered. (biomedcentral.com)

Mesenchymal stromal cells (MSCs) are cells of non-haematopoietic origin, with the capacity to differentiate into multiple lineages of the mesenchyme, that is, chondrocytes, osteoblasts and adipocytes. (bmj.com)

Chronic lymphoid leukaemia and hairy cell leukaemia due to chronic exposure to benzene: Report of three cases. (cdc.gov)
Within the normal lymphoid system, HDAC9 is co-expressed with BCL-6 in germinal center B-cells (∼60% of cells). (sunderland.ac.uk)
The combined knockout of results in reduced amount of lymphoid and myeloid cells. (irpa2006europe.com)
[ 12 ] These cells can capture antigen and migrate to lymphoid organs, where they present the antigens to naive T cells. (medscape.com)

2 MSCs were first described in the bone marrow where they constitute a small fraction of cells (0.001%-0.01%) that closely interact with haematopoietic cells to support haematopoiesis and skeletal homeostasis. (bmj.com)

Siglecs (sialic acid-binding immunoglobulin-like lectins) are single-pass cell surface receptors that have inhibitory activities on immune cells. (mdpi.com)
Following early observations that MSCs inhibit T-cell proliferation, 9 MSCs were found to interact with the majority of innate and adaptive immune cells. (bmj.com)
This review further emphasizes the diversified cross-talk between γδ T cells and other immune cells. (explorationpub.com)
In innate immunity, immune cells are activated by their pattern recognition receptors (PRRs), which bind to pattern molecules of invading pathogens. (fortunejournals.com)
The TLRs and CLRs of the immune cells work in coordination with the immune system. (fortunejournals.com)

Cobalt bioaccumulation in mouse blood plasma and liver. (bsb-bg.eu)

A viral infection following Japanese encephalitis virus infection also increased GPR84 expression by 2-4.5% in the mice brain. (wikipedia.org)
Elevated expression of GPR84 was also observed during the demyelination phase of the reversible Cuprizone-Induced Demyelinating Disease mouse model. (wikipedia.org)
Expression of GPR84 increases in mouse whole brain samples from experimental autoimmune encephalomyelitis before the onset of clinical disease. (wikipedia.org)
GPR84 expression was increased by 49.9 times in M1 type macrophages isolated from aortic atherosclerotic lesions of LDLR-/- mice were fed a western diet. (wikipedia.org)
Taken together these data suggest that HDAC9 plays a role in B-cell maturation and its ectopic expression in early B-cells leads to perturbation of normal B-cell development, possibly predisposing transgenic mice to tumorigenesis. (sunderland.ac.uk)
HTS revealed 1,2,3,4-tetrazolo[1,5-b]pyridazine-based compounds as efficient transcriptional inhibitors of MACC1 expression, able to decrease MACC1-induced cancer cell motility in vitro. (bvsalud.org)
Flow cytometric analysis of CD43 expression on mouse splenocytes. (bdbiosciences.com)
Furthermore, we show expression of MSTN by CML mesenchymal stromal cells, and that myostatin propeptide has a direct and instant effect on CML cells, independent of myostatin, by demonstrating binding of myostatin propeptide to the cell surface and increased phosphorylation of STAT5 and SMAD2/3. (haematologica.org)
While the mechanisms responsible for aberrant plasma cell expression of CXCL12 remain to be determined, studies in other systems suggest a role for hypoxia and hypoxia-inducible transcription factors. (haematologica.org)
Design and Methods The expression of hypoxia-inducible factor protein was examined in patients' bone marrow biopsy specimens using immunohistochemistry. (haematologica.org)
Results Strong hypoxia-inducible factor-2 protein expression was detected in CD138 + multiple myeloma plasma cells in patients' biopsy specimens. (haematologica.org)
Prolonged exposure to hypoxia strongly up-regulated CXCL12 expression in multiple myeloma plasma cells and hypoxia-inducible factor-2 was found to play a key role in this response. (haematologica.org)
12 It is well established that hypoxia is an important selective force in the evolution of tumor cells, 13 and elevated expression of the hypoxia-inducible transcription factors HIF-1 and HIF-2 has been documented in several human cancers. (haematologica.org)
Co-expression of two mutant genes increased myeloid stem cells in animal model, suggesting that cooperation of RUNX1 and ASXL1 mutations played a critical role in leukemia transformation. (biomedcentral.com)

When stimulated to divide, they give rise to more committed, differentiated daughter cells with less proliferative potential, called "early progenitor" cells. (justia.com)

VetStem Regenerative Cell Therapy is based on a clinical technology licensed from Artecel Inc. Original patents are from the University of Pittsburgh and Duke University. (vetstem.com)
Yet, CD133 + stem cells for clinical use are purified using manual or semi-automatic devices based on the conventional magnetic cell sorting (MACS®) technique. (biomedcentral.com)
Fusion cell vaccination of patients with metastatic breast and renal cancer induces immunological and clinical responses. (nwbio.com)
However, the clinical application of tumor-infiltrating γδ T cells has certain limitations. (explorationpub.com)
Finally, it retrospectively analyze the activation strategies and clinical relevance of existing γδ T cell adoptive immunotherapies. (explorationpub.com)

We are studying the effects and consequences of protein fusion on the transcriptional and transforming activities of these proteins using in vitro and animal models. (stanford.edu)
Ly6G is a 21-25 kDa member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. (bioxcell.com)
[ 15 ] The surface of the APC contains 2 peptide-binding proteins (ie, major histocompatability complex [MHC] classes I and II), which can stimulate cytotoxic T (T C ) cells, regulatory T (Treg) cells, and helper T (T H ) cells. (medscape.com)

Each category of histiocytosis can be traced to reactive or neoplastic proliferation in one of these cell lineages. (medscape.com)

Why Use Adipose-Derived Stem Cells? (vetstem.com)
uses a concentrated form of autologous adipose-derived adult stem cells to treat traumatic and degenerative diseases, including bowed tendons, ligament injuries, osteoarthritis, and osteochondral defects in horses, dogs, and cats. (vetstem.com)
Why use adipose-derived regenerative cells rather than regenerative cells derived from bone marrow? (vetstem.com)

Stem cells represent a very small percentage of bone marrow cells, and are normally quiescent. (justia.com)

Mesenchymal stromal cells: a novel therapy for the treatment of chronic obstructive pulmonary disease? (bmj.com)

MSCs played a crucial role in regenerative therapy and have been introduced as an interdisciplinary field between cell biology and material science. (techscience.com)

Taking advantage of the diverse γδ TCR repertoire or other ligand-receptor interactions, γδ T cells can recognize a broad spectrum of tumor-associated antigens (TAAs) in a major histocompatibility complex (MHC)-independent manner, thereby activating downstream pleiotropic effects. (explorationpub.com)

Surprisingly, MPL515/625 also caused hypercellularity of bone marrow and splenomegaly without any other significant features. (biomedcentral.com)
At 9 months post-BMT, mice harboring combined RUNX1 and ASXL1 mutations developed disease characterized by marked splenomegaly, hepatomegaly, and leukocytosis with a shorter latency. (biomedcentral.com)

Furthermore, we describe a subset of myeloid cells with immunosuppressive activity (known as myeloid-derived suppressor cells). (hindawi.com)

We employ a variety of experimental systems for our studies ranging from molecular biology to transgenic and knockout mice. (stanford.edu)

Ablating lysosomal acid lipase (Lal-/-) in mice led to aberrant expansion of myeloid-derived suppressive cells (MDSCs) (>40% in the blood, and >70% in the bone marrow) that arise from dysregulated production of myeloid progenitor cells in the bone marrow. (wikipedia.org)

The 1A8 monoclonal antibody reacts with mouse Ly6G. (bioxcell.com)
Unlike the RB6-8C5 antibody, the 1A8 antibody reacts specifically with mouse Ly6G with no reported cross reactivity with Ly6C. (bioxcell.com)
The production of luminol signal was not associated with the influx of Ly6G(+) cells, but was related to oxidative burst, as an indicator of neutrophil function. (bioxcell.com)
Taken together, this research supports an important role for early activity by Ly6G(+) cells in the generation of long-term PDT responses in mesothelioma, and it points to luminol chemiluminescence as a potentially useful approach for preclinical monitoring of neutrophil activation by PDT. (bioxcell.com)

Immunophenotypic analysis of B-cells isolated from bone marrow (BM) revealed an absence of cells expressing the pre-B/immature-B cell markers normally associated with C-E Hardy's fractions. (sunderland.ac.uk)

Hemizygous and homozygous mice expressing Flag epitope tagged human HDAC9 (fHDAC9) transgene display throughout their lifespan altered B-cell development. (sunderland.ac.uk)
NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
SummaryThe ability to integrate information across multiple datasets represents an enormous opportunity for the human cell atlas (HCA), yet presents substantial computational challenges. (authorea.com)
Here we aim to address a fundamental question for the human cell atlas: how can we integrate a diverse community effort to study a complex human system effectively in order to construct a coherent atlas? (authorea.com)
Emerging applications of recombinant human granulocyte-macrophage colony-stimulating factor. (nwbio.com)
At present, the phenotypic heterogeneity and functional variation of human γδ T cells are increasing. (explorationpub.com)
In this review, we use the Heilig nomenclature to describe human γδ T cells [ 1 ]. (explorationpub.com)

However, it remains unclear whether there is functional crosstalk between these two types of skeletal stem cells. (nature.com)
γδ T cells express unique T cell receptor (TCR) γ and TCR δ chains, with structural and functional heterogeneity. (explorationpub.com)

We propose that powerful machine-learning techniques based on 'joint manifold learning', often used in the 'alignment' of massive imaging datasets to recognized shared high-dimensional features, can be used to recognize shared cellular phenotypes across single cell datasets. (authorea.com)
Additionally, these cells show highly flexible and dynamic plasticity and are extremely easily polarized into regulatory phenotypes. (explorationpub.com)

To determine whether viral effect(s) occurred in adherent stromal and/or non-adherent hematopoietic stem cells, purified cell populations, comprised exclusively of cells from each compartment, were separated from C57BL/6J (Fv-1 bb ) or C3H/HeJ (Fv-1 nn ) marrow cultures and were left uninfected or were infected with host range replication restricted B-tropic or N-tropic helper pseudotype viruses of SFFV respectively. (johnshopkins.edu)

Adult stem cells remain of particular interest as therapeutic agents for cardiac regeneration in ischemic heart disease [ 1 - 4 ]. (biomedcentral.com)
Bone marrow was aspirated from the superior iliac crest of two healthy adult female volunteers. (bmj.com)

Anatomy41

Organisms13

Diseases12

Chemicals and Drugs42

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Phenomena and Processes36

Disciplines and Occupations1

Information Science2

Monocytes16

Dendritic cells18

Assays7

Transplantation8

Multipotent4

Endothelial14

Hematopoiesis9

Eosinophils7

Differentiation16

Vivo7

Cytokines7

CD348

Peripheral blood10

Lymphocytes2

Microenvironment5

Normal bone marrow1

Mast cells1

Hematopoietic stem2

Lineage4

Colony formation6

Stem cell12

Tissue14

Tissues6

Clonal4

Subsets6

Angiogenesis11

Erythroid3

Inflammatory12

Colonies1

Tumors10

Platelets1

HSPCs2

Pluripotent3

Gene8

Mononuclear1

Subsequent3

Spleen3

Vascular2

Differentiate into multiple lineages1

Lymphoid4

Haematopoietic1

Immune cells5

Liver1

Expression14

Proliferative potential1

Clinical5

Proteins3

Lineages1

Adipose3

Quiescent1

Stromal cells1

MSCs1

Tumor-associated1

Splenomegaly2

Suppressor cells1

Transgenic1

Myeloid-derived1

Ly6G4

Immature1

Human7

Functional2

Phenotypes2

C57BL1

Adult2