Mirna oligonucleotide probes. Medical search. Frequent questions

MicroRNAs (miRNAs or miRs) play an active role in the regulation of gene expression by modifying mRNA stability and translation, usually through specific albeit partial complementary binding to the 3′ untranslated region. (nature.com)
MicroRNAs (miRNAs) are a class of recently discovered noncoding RNA genes that post-transcriptionally regulate gene expression. (nih.gov)
MicroRNAs (miRNAs) are 21-24 nt long non-coding RNAs that regulate gene expression at the post-transcriptional level. (researchsquare.com)
Regulatory noncoding microRNAs (miRNAs) play a critical role in the regulation of gene expression [ 5 ] and are potential candidates for studying their role in the progression of PCa to androgen-independent stage. (biomedcentral.com)
MicroRNAs (miRNAs) are potent effectors in gene regulatory networks where aberrant miRNA expression can contribute to human diseases such as cancer. (hindawi.com)
Although microRNAs (miRNAs) are physically small, they have been shown to play an important role in gene regulation [ 1 ]. (hindawi.com)
MicroRNAs (miRNAs), a recently identified class of endogenous, small (~22 nt), non-protein coding, single-stranded RNA molecules, negatively regulate protein-coding genes by base-pair matching with the 3' UTRs of mRNAs. (biomedcentral.com)
MicroRNAs (miRNAs) are small noncoding RNAs regulating gene expression by mRNA targeting. (biomedcentral.com)
MicroRNAs (miRNAs) have emerged as important post-transcriptional regulators of gene expression in many developmental and cellular processes. (biomedcentral.com)
MicroRNAs (miRNAs) are an abundant class of small (approximately 22 nt) endogenous non-coding RNAs that direct post-transcriptional regulation of gene expression. (biomedcentral.com)
MicroRNAs (miRNAs) are associated with cytogenetics and molecular subtypes of acute myelogeneous leukemia (AML), but their impact on AML pathogenesis is poorly understood. (ashpublications.org)
Detections of small non-coding RNAs such as microRNAs and short oligonucleotide therapeutics such as ASO and siRNA require a robust, highly specific and sensitive assay with minimal time and effort, , delivering data for easy interpretation. (acdbio.com)
MicroRNAs (miRNAs) are well-recognized for their abilities to regulate gene expression post-transcriptionally in plants and animals. (frontiersin.org)
The invention comprises "a method of diagnosing, assessing susceptibility, and/or treating schizophrenia involving the identification and/or observation of microRNAs and variant miRNA," according to the patent's abstract. (genomeweb.com)
In our study, we proposed that hypoxic preconditioning could increase survival of bone marrow mesenchymal stem cells (MSCs) via upregulation of Pim-1 and aimed to determine some microRNAs (miRNAs) that modulate the expression of Pim-1. (biomedcentral.com)

In biology, a probe is a single strand of DNA or RNA that is complementary to a nucleotide sequence of interest. (wikipedia.org)
Complementary oligonucleotides capable of hybridizing to 181 miRNAs were immobilized on a membrane and probed with radiolabeled RNA derived from low molecular weight fractions of total RNA from several different hematopoietic and neuronal cells. (nih.gov)
miRNAs control gene expression by binding to the complementary sites in the 3' untranslated regions (3' UTRs) of target mRNAs and triggering either translational inhibition or mRNA degradation by a molecular mechanism which is a subject of intense investigation [ 6 - 9 ]. (biomedcentral.com)
This was developed by immobilization of a thiolated oligonucleotide, fully complementary to the miRNA-21 sequence, on the disposable gold electrode. (uam.es)
Incorporation of miRNAs in RNA-induced silencing complexes (RISCs) then leads to decay or to translational repression of complementary mRNA targets. (biomedcentral.com)
Metazoan miRNAs guide the miRISC to partially complementary sites in the 3' UTRs of target mRNAs to promote their translational repression or deadenylation and degradation. (biomedcentral.com)
Annealing is the process of heating and cooling two single-stranded oligonucleotides with complementary sequences. (sigmaaldrich.com)
The ratio of quantum yields between after and before addition of the complementary nucleic acid strands showed a strong relationship with the number of possible Watson-Crick base pairs formed within two bases from D 514 in the 'self-dimer' of the probe. (ne.jp)
The target of ECHO probes is only the complementary single-stranded nucleic acid. (ne.jp)
Compared to standard complementary probes, the signal we obtained using ACD Bio was evident and specific. (acdbio.com)
short oligonucleotides complementary to amplicons, which fluoresce during PCR (under degradation). (unige.ch)

Balanced oligonucleotide solutions for the analysis of a specific miRNA molecule: RT primer, a pair of PCR primers and a fluorescently labeled probe for amplification detection. (algimed-techno.com)
A standard PCR consists of target DNA, a set of synthetic oligonucleotide primers that flank the target DNA sequence, a thermostable DNA polymerase (usually Taq polymerase), and nucleotides. (sigmaaldrich.com)
Applied Biosystems predesigned TaqMan Assays consist of target-specific primers and one or more probes optimized for specific applications, including gene expression, SNP genotyping, miRNA, mutation detection, and copy number variation analysis. (thermofisher.com)
Design your own TaqMan primer and probe sets from your choice of dye-labeled TaqMan MGB, TAMRA, or QSY custom probes and Applied Biosystems unlabeled, sequence detection primers. (thermofisher.com)
Primers and probes are HPLC purified and verified by ESI mass spectrometry, delivering batch-to-batch consistency to provide confidence in your data. (idtdna.com)
1) Oligonucleotide Primers. (srnaprimerdb.com)
Furthermore, LNA® offers the possibility to adjust Tm values of primers and probes in multiplex assays. (eurogentec.com)
Double-Dye Oligonucleotides are designed to anneal between the upstream and downstream PCR primers. (eurogentec.com)

A simple carbon nanodot-based electrogenerated chemiluminescence biosensor is described for sensitive and selective detection of microRNA-21 (miRNA-21), a biomarker of several pathologies including cardiovascular diseases (CVDs). (uam.es)
Sensitive quantification of microRNA (miRNA) plays a crucial role in early diagnosis and precise therapy of osteosarcoma. (springeropen.com)
MicroRNA (miRNA) is a kind of small non-coding RNAs with the function of modulating gene expression, which is involved in diverse pathological processes, e.g., cancers (Acunzo et al. (springeropen.com)
microRNA alleles associated with schizophrenia and schizophrenia spectrum disorders were identified and ultra-rare variants in the precursor or mature miRNA were identified. (genomeweb.com)
October 1, 2021 -- Using a new nanoscale sequencer, a research group has sequenced a microRNA (miRNA) that is a marker for refractory gastrointestinal cancer. (scienceboard.net)
We further predicted that microRNA-206 (miR-206) is one of the potential miRNAs that targets Pim-1 through TargetScan, microRNA, and miRDB software. (biomedcentral.com)

Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only particular parts of a nucleic acid sequence with a high degree of sequence complementarity. (wikipedia.org)
The hybridization signals for each probe when a nucleic abnormality is detected. (wikipedia.org)
After checking all the necessary conditions, hybridization steps can be started by first adding a target-specific probe, composed of 20 oligonucleotide pairs, hybridizes to the target RNA(s). (wikipedia.org)
Existing methods for measuring transcript levels in single cells include RT-qPCR (1), single molecule counting using digital PCR (2) or hybridization probes (3, 4), and next generation sequencing (5). (justia.com)
Synthetic oligonucleotides with covalently-attached CDPI3 have enhanced DNA affinity and have improved the hybridization properties of sequence-specific DNA probes. (genelink.com)
Paraffin-embedded glioma specimens were used for MiRNA locked nucleic acid (LNA) in situ hybridization and immunohistochemistry, and frozen glioma specimen were used for Real-time PCR. (biomedcentral.com)
The target miRNA-21 was hybridized with the probe on the electrode surface, and the hybridization was detected by the enhancement of the [Ru(bpy)3]2+/DNA ECL signal using CNDs. (uam.es)
Hybridization with oligonucleotide in the picomolar regime induces a sizable reduction of the current flowing through the transistor channel. (unimore.it)
In this web page, recent advances in nucleic acid detection using exciton-controlled hybridization-sensitive fluorescent oligonucleotide (ECHO) probe technologies are introduced to you. (ne.jp)
In ECHO probes, a hybridization-dependent fluorescent nucleotide regulated by the H-aggregation of thiazole orange organic dyes (D 514 ) is incorporated into specific sequence contexts and serves as fluorescent detection readout for target nucleic acids. (ne.jp)
The miRNAscope™ Assay is a highly sensitive and specific in situ hybridization assay that allows for the visualization of ASO, miRNA, siRNA and other nucleic acid targets between 17-50 nucleotides expression in intact tissues or cultured cells with single-cell resolution, preserving spatial and morphological context. (acdbio.com)
When incorporated into a probe, locked nucleic acids impart heightened structural stability to the target sequence leading to increased hybridization temperature. (idtdna.com)

It is becoming clear that miRNAs play an important role in the regulation of gene expression during development. (nih.gov)
Gene expression modulating miRNAs are encoded in diverse genomic locations including intergenic regions, introns of protein-coding genes and introns/exons of noncoding RNA genes [ 36 ]. (biomedcentral.com)
For a better understanding of the regulatory role of miRNAs in coordinating gene expression, we here present a systems biology approach combining data-driven modeling and model-driven experiments. (hindawi.com)
Furthermore, as many applications, like genotyping or gene expression profiling, are requiring small amounts of several different probes, Eurogentec has developed a high-throughput platform to produce high quality small scale Real-Time qPCR Double-Dye probes. (eurogentec.com)

FISH can also be used to detect and localize specific RNA targets (mRNA, lncRNA and miRNA)[citation needed] in cells, circulating tumor cells, and tissue samples. (wikipedia.org)
RNA probes can be designed for any gene or any sequence within a gene for visualization of mRNA, lncRNA and miRNA in tissues and cells. (wikipedia.org)
Each probe for the detection of mRNA and lncRNA is composed of ~20-50 oligonucleotide pairs, each pair covering a space of 40-50 bp. (wikipedia.org)
It is likely that the intronic miRNAs are processed from the same primary transcript as the precursor mRNAs and thus, their expression levels are regulated by the expression of the host mRNA [ 39 ]. (biomedcentral.com)
Recently, miRNA-messenger RNA (mRNA) regulatory relationships have been confirmed during biological processes, including osteogenic differentiation. (frontiersin.org)
This study aimed to find out more candidate miRNA-mRNA pairs involved in the osteogenic differentiation of MC3T3-E1 cells. (frontiersin.org)
Multiple miRNA-mRNA prediction databases were searched by differentially expressed genes (DEGs) to obtain pairs of a miRNA-DEG regulatory network. (frontiersin.org)
The miRNA-mRNA interactions were verified by dual-luciferase reporter gene assays and experiments using mimics miRNA or their inhibitors. (frontiersin.org)
There were 7 overlapped miRNA-mRNA pairs identified during osteogenic differentiation of MC3T3-E1 cells, including mmu-miR-204-5p-Arhgap11a, mmu-miR-211-5p-Arhgap11a, mmu-miR-24-3p-H2afx, mmu-miR-3470b-Chek2, mmu-miR-3470b-Dlgap5, mmu-miR-466b-3p-Chek1, and mmu-miR-466c-3p-Chek1. (frontiersin.org)
Such RNA base modifications, which are implemented through the biochemical process of methylation, have been identified in both mRNA, which codes for proteins, and miRNAs, which are small noncoding RNA molecules that play a regulatory role by suppressing the expression of a gene. (scienceboard.net)
DNA microarrays take advantage of the major feature of the DNA double helix, the sequence complementarity of the two-paired strands, by using DNA capture probes which are the complement of the expressed target sequence (mRNA, cRNA or cDNA made from the mRNA). (ddw-online.com)
They are considered to be next-generation drugs due to two important factors: firstly, they act not only on proteins that have traditionally been targeted by conventional small-molecule or antibody base drugs, but also on functional RNA such as mRNA and miRNA, and secondly they can be manufactured by chemical synthesis, which means that they can be easily mass produced. (jascoinc.com)

We used oligonucleotide arrays to analyze miRNA expression in the murine hematopoietic system. (nih.gov)
For the identification of deregulated miRNAs we applied miRNA microarrays which have been widely used to analyze miRNA expression patterns. (biomedcentral.com)

For miRNA detection, the probes use proprietary chemistry for specific detection of miRNA and cover the entire miRNA sequence. (wikipedia.org)
Optimization of the RT-primer structure and detection of amplification using a probe ensures reliable results and higher sensitivity of the assay compared to other technologies (Korobkina et al. (algimed-techno.com)
These results paved the way for the development of a sensitive ECL biosensor for the detection of miRNA-21. (uam.es)
The biosensor shows a linear response to miRNA-21 concentration up to 100.0 pM with a detection limit of 0.721 fM. (uam.es)
Multicolor detection and auxiliary functional modules have been built into ECHO probes to accommodate a broad range of biological applications. (ne.jp)
The conventional method for miRNA detection is qRT-PCR, which is the gold standard for clinical and laboratory trials (Hu et al. (springeropen.com)
Based on this, diverse PER-based miRNA detection approaches have been proposed. (springeropen.com)
With this in mind, we depict here a label-free miRNA detection approach with an improved sensitivity by combining split-DNAzyme-based signal recycle with PER and using G-duplex for NMM-based signal generation. (springeropen.com)
Since the sequencer relies on individual conductance profiles rather than chemical probes or polymerase chain reaction amplifications, it was possible to detect both methylations, marking the first time that simultaneous detection of m6A and 5mC in the same miRNA molecule had been achieved. (scienceboard.net)

Nucleic acids, such as antisense oligonucleotides (AONs) and small interfering RNAs (siRNAs) have enormous potential as therapeutics for life-threatening diseases because in principle, any problematic gene can be targeted with high efficacy and sequence-specificity. (grstiftung.ch)

Short CDPI3-oligonucleotides hybridize with single-stranded DNA to give more stable DNA duplexes than unmodified ODNs of similar length. (genelink.com)
They act as co-reactant agents in the anodic electrochemiluminescence (ECL) of [Ru(bpy)3]2+, producing different signals for the probe (single-stranded DNA) and the hybridized target (double-stranded DNA). (uam.es)
However, even this newly designed fluorescent probe exhibits a sequence-dependent change in the ratio of the fluorescence intensities for double- and single-stranded states. (ne.jp)

Applied Biosystems TaqMan predesigned assays contain everything you need: TaqMan probe and PCR primer sets formulated to work right out of the box. (thermofisher.com)
With their expertise and through clear communication, we developed several assays to detect both RNA and short oligonucleotides in tissue. (acdbio.com)

Chemically modified antimiR oligonucleotides sequester the mature miRNA in competition with cellular target mRNAs leading to functional inhibition of the miRNA and derepression of the direct targets. (biomedcentral.com)

1 , 2 Most miRNAs are predicted to regulate several hundred mRNAs, and many mRNAs are regulated by multiple miRNAs. (nature.com)
The expressions of candidate miRNAs and mRNAs were determined by real-time quantitative PCR(RT-qPCR) in MC3T3-E1 cells. (frontiersin.org)

This protocol describes the detailed experimental procedure for stem-loop poly(U) tailed RT-PCR using SYBR Green I. The procedure begins with reverse transcription of poly(U) tailed total RNA, or small RNA-enriched RNA, miRNA, siRNA, etc. (srnaprimerdb.com)

Therefore, the role of miRNAs and their differential signature expression patterns in androgen-dependent and androgen-independent PCa cells requires a closer validation. (biomedcentral.com)

Aberrant miRNA expression in smokers compared to non-smokers and COPD compared to normal was analyzed using microarray datasets and reverse-transcriptase quantitative polymerase chain reaction (qPCR). (researchsquare.com)
miRNA genes are transcribed by RNA polymerase II into long primary miRNA transcripts, termed pri-miRNAs that are usually several kilobases long and possess a 5' CAP and a poly(A) tail. (biomedcentral.com)
3' exonuclease activity of the Taq DNA polymerase cleaves the fluorophore from the probe. (eurogentec.com)

Pre-miRNAs are exported to the cytoplasm by Exportin-5 and processed further by Dicer, to ~22 nt double-stranded miRNA duplexes that are loaded into an Argonaute protein in the miRISC and rapidly unwound. (biomedcentral.com)
The ColoR9 MB was synthesized by way of a partly double-stranded Genetics oligonucleotide that contain a miR-9 presenting web site and a news reporter probe along with Cy3/black opening quencher 1 (BHQ1) on one side plus a reference probe with Cy5.Five in the opposite end. (mglursignaling.com)

Finally, we summarize recent progress in antimiR mediated pharmacological inhibition of disease-associated miRNAs, which shows great promise in the development of novel miRNA-based therapeutics. (biomedcentral.com)
In recent years the use of oligonucleotide based therapeutics derived from nucleic acids such as DNA or RNA, has increased significantly. (jascoinc.com)
Oligonucleotide therapeutics are mainly based on artificial oligonucleotides that are chemically modified DNA or RNA, which improves their structural stability in the human body and affinity to their target molecules 1 ). (jascoinc.com)

4 , 5 , 6 The idea, however, that miRNAs may play an important role in some human disease/disorders is gaining momentum, and as these molecules have the ability to silence many genes simultaneously, dysregulation of even a single miRNA can have a significant polygenic effect. (nature.com)
A large number of miRNAs are encoded in organized intronic clusters within many protein coding genes. (biomedcentral.com)
Here we have examined the expression of mature miRNAs of miR 17-92, miR 106b-25 and miR 23b-24 clusters along with their host genes C13orf25, MCM7 and AMPO respectively in PCa cell lines. (biomedcentral.com)
The expression profiling of miRNAs and host genes was performed in androgen-sensitive MDA PCa 2b and LNCaP as well as in androgen-refractory PC-3 and DU 145 cell culture models of PCa. (biomedcentral.com)
We found significant expression variations between host genes and their resident miRNAs. (biomedcentral.com)
The expressions of C13orf25 and miR 17-92 cluster as well as MCM7 and miR 106b-25 cluster did not reveal statistically significant correlation, thus suggesting that host genes and resident miRNAs may be expressed independent of each other. (biomedcentral.com)
miRNAs are either expressed from independent transcriptional units or derive from introns of protein-coding genes or exons or introns of long ncRNAs. (biomedcentral.com)

probe sequence. (genelink.com)
Use our TaqMan Custom Design Assay Tool to easily design your own assay based on your target sequence of interest or submit the primer/probe sequences of your own design. (thermofisher.com)
Prices listed include probe sequence (10-25 standard bases with up to 6 locked nucleic acid nucleotides), reporter, quencher, and HPLC purification. (idtdna.com)

Recently, the lack of miRNA processing has been shown to promote neurodegeneration and deregulation of several miRNAs has been reported to be associated with Scrapie in mice. (biomedcentral.com)
Recent studies have shown that several miRNAs are involved in the regulation of vascular development [ 5 , 6 , 7 ]. (oncotarget.com)

To demonstrate the application of this approach, we adopt it to investigate mechanisms of collective repression on p21 by multiple miRNAs. (hindawi.com)
The application of the systems biology approach to the analysis of a gene regulatory network is demonstrated with a case study of the regulation of p21 by multiple miRNAs [ 4 ]. (hindawi.com)

More information about probes labeled with the SUN fluorophore can be found on the PrimeTime™ qPCR probes page or the SUN fluorophore Decoded™ article . (idtdna.com)
Functional data can be found on the Affinity Plus qPCR Probes page . (idtdna.com)

Because miRNAs are only present in tiny amounts in the body they can be difficult to detect using standard systems, making the use of Exiqon's LNA probe technology that binds very strongly to RNA and DNA targets highly desirable. (outsourcing-pharma.com)
Most experimental approaches focus on the identification of miRNA targets and the investigation of physiological consequences when perturbing miRNA expressions but are unsuited to provide a system-level interpretation for observed phenomena. (hindawi.com)
The network combining putative targets of TF and miRNA regulation with experimentally proven molecular interactions was constructed and visualized. (hindawi.com)
These findings indicate that TIMP3 is a critical target of miR-221 and miR-222 and that these two miRNAs could be critical therapeutic targets and survival predictors in glioma. (biomedcentral.com)
Indeed, disease-associated miRNAs represent a new class of targets for the development of miRNA-based therapeutic modalities, which may yield patient benefits unobtainable by other therapeutic approaches. (biomedcentral.com)

Set of eight calibrators - serial dilutions of a synthetic analogue of the detected miRNA molecule. (algimed-techno.com)
Together, the data support a tumor suppressor role for miR-29 and provide a rationale for the use of synthetic miR-29b oligonucleotides as a novel strategy to improve treatment response in AML. (ashpublications.org)

The patent, its abstract states, relates to "duplex oligonucleotide complexes [that] can be administered to a cell, tissue or organism to silence a target gene without the aid of a transfection reagent. (genomeweb.com)

The all-share acquisition of Oncotech for around €31m will enable Exiqon to combine its highly sensitive locked nucleic acid (LNA) oligonucleotide probe technology and microarray platforms with Oncotech's expertise in developing nucleic acid-based diagnostic tests using its bank of tumour samples. (outsourcing-pharma.com)
These locked nucleic acid probes are commonly used for genotyping and custom applications such as transcript variant identification or the differentiation of microbial species. (idtdna.com)
Locked nucleic acid nucleotides can be incorporated into effective qPCR probes [ 1-4 ]. (idtdna.com)

The probe self-dimer must be thermodynamically unstable because it contains many mismatched base pairs, but this plausible self-dimeric structure may inhibit fluorescence quenching because the formation of a 'primer dimer' in PCR experiments significantly decreases amplification efficiency. (ne.jp)
Target miRNA cooperates the activation of split-DNAzyme with Mg 2+ through assisting the assembly of DNAzyme to correct conformation, which enables the performance of PER-based nucleic acids amplification to produce a large amount of single-strand DNA (ssDNA) sequences. (springeropen.com)

The stem-loop poly(U) tailed RT-PCR method described here is designed to detect and quantify sncRNAs, especially for miRNAs. (srnaprimerdb.com)

In this method, sncRNA of the same sample are polyuridylated and reverse transcribed into cDNAs using a universal stem-loop poly(A) RT primer and then used as templates for SYBR Green or TaqMan probe real-time PCR (Fig 1). (srnaprimerdb.com)

PrimeTime qPCR Probes are 5′ nuclease probes, available with an assortment of reporter-dye combinations that are compatible with common qPCR instruments and that include several license-free combinations. (idtdna.com)

MiRNAs are derived from primary transcripts (pri-miRNA) that are sequentially processed into their mature form by the RNase III type nucleases DROSHA and DICER [ 5 ]. (biomedcentral.com)

MiRNAs are dysregulated in tumors and function as oncogenic miRNAs or as tumor suppressor miRNAs [ 4 ]. (biomedcentral.com)
Growing evidence has indicated important roles for miRNAs in tumor invasion. (biomedcentral.com)
miRNA-378 promotes tumor angiogenesis by targeting Sufu and Fus-1 [ 8 ]. (oncotarget.com)
For example, Zhang built a tumor-associated biomarker initiated PER-based DNA machine and successfully applied it for miRNA imaging and gene therapy (Zhang et al. (springeropen.com)

PrimeTime Mini LNA qPCR Probes are ideal for screening a small sample set or performing a few reactions to optimize probe designs. (idtdna.com)
PrimeTime LNA qPCR Probes are offered with a wider selection of dyes and quenchers than PrimeTime Mini LNA qPCR Probes, and are best-suited for large-scale or high-throughput applications. (idtdna.com)
Because these bases significantly increase melting temperature (T m ), PrimeTime LNA qPCR Probes can be designed with shorter lengths than standard qPCR probes. (idtdna.com)
PrimeTime LNA qPCR Probes can be ordered either at a guaranteed yield of 0.5 nmol or at defined synthesis scales to suit your research needs. (idtdna.com)
What is the difference between the locked nucleic acids in Affinity Plus™ qPCR Probes and PrimeTime™ LNA ® qPCR Probes? (idtdna.com)
There are no structural differences between the locked nucleic acids used in Affinity Plus and PrimeTime LNA* qPCR Probes. (idtdna.com)
Have questions about PrimeTime qPCR Probes? (idtdna.com)
For more information, download the PrimeTime Custom qPCR Probes Flyer . (idtdna.com)

Probes are often derived from fragments of DNA that were isolated, purified, and amplified for use in the Human Genome Project. (wikipedia.org)
These fragments are on the order of 100 thousand base-pairs, and are the basis for most FISH probes. (wikipedia.org)

In this review, we focus on the use of antisense oligonucleotides (antimiRs) in miRNA inhibition for loss-of-function studies. (biomedcentral.com)
We provide an overview of the currently employed antisense chemistries and their utility in designing antimiR oligonucleotides. (biomedcentral.com)
Additional purification significantly improves oligonucleotide performance in demanding applications such as multiplex PCR, cloning, mutagenesis, and antisense/RNAi methods. (mbiotech.co.kr)

The aim of this study is to investigate the key regulatory miRNA-486-5p and underlying molecular mechanisms in chronic obstructive pulmonary disease (COPD) progression. (researchsquare.com)
Research advances have demonstrated the crucial regulatory roles of miRNAs in many diseases, including COPD [ 13 , 14 ]. (researchsquare.com)
Currently, an increasing number of studies are being carried out to deepen our understanding of miRNA regulatory mechanisms and functions. (hindawi.com)
The systems biology approach, combining data-driven modeling and model-driven experiments, provides a systematic and comprehensive perspective on the regulatory roles of miRNAs in gene regulatory networks [ 3 - 5 ]. (hindawi.com)

miRNAs have been shown to have a surprisingly widespread effect on gene regulation, with some studies indicating they may regulate more than 30 per cent of the human genome. (outsourcing-pharma.com)

Each custom assay is a mix of forward primer, reverse primer, and FAM and/or VIC dye-labeled TaqMan MGB probe. (thermofisher.com)
TaqMan probes provide additional level of specificity compared to Sybr Green . (unige.ch)

As expected, miRNA expression patterns were very different between hematopoietic and non-hematopoietic cells, with further subtle differences observed within the hematopoietic group. (nih.gov)

Fluorescence microscopy can be used to find out where the fluorescent probe is bound to the chromosomes. (wikipedia.org)
End point PCR using fluorescent-labeled probes (or Sybr Green ) is done on chips containing 20K partitions. (unige.ch)
Eurogentec is a supplier of Double-Dye Oligonucleotide probes and offers the largest number of fluorescent reporters and quenchers. (eurogentec.com)
Double-Dye Oligonucleotide probes with various non-fluorescent quenchers and a variety of fluorescent dyes can be conveniently ordered using our web ordering interface. (eurogentec.com)
Double-Dye Oligonucleotides have a fluorescent reporter dye and a quencher at their 5' and 3' ends, respectively. (eurogentec.com)

Earlier this year , researchers found that three miRNA molecules play an important part in regulating the p53 tumour suppressor network, suggesting new approaches for the diagnosis and treatment of various cancers. (outsourcing-pharma.com)

2021 ). Recent researches implied the crucial roles of miRNA in regulating the proliferation and differentiation of osteosarcoma cells and hold the view that miRNA could be potentially applied for the diagnosis, prognosis and treatment of osteosarcoma (Sekar et al. (springeropen.com)

The aim of our study was to analyze the differential expression of miRNAs in the brains of BSE-infected cynomolgus macaques as a model for Creutzfeldt-Jakob disease (CJD). (biomedcentral.com)

They are designed using a highly sophisticated oligonucleotide probe/primer design pipeline including robust primer design algorithms and an extensive array of bioinformatics tools and processes to automate assay design. (thermofisher.com)
Herein, we build a label-free and sensitive miRNA quantification approach based on the activation of split-DNAzyme initiated primer exchange reaction (PER). (springeropen.com)
Save the time spent designing your own and use one of our predesigned qPCR primer/probe sets, listed below. (idtdna.com)
The Tm of the Double-Dye Oligonucleotide probe must be higher than the upstream primer, to avoid that the later primer is already being extended while the Double-Dye Oligonucleotide probe is not yet annealed. (eurogentec.com)

"Exiqon's state-of-the-art miRNA tools will allow Oncotech to identify molecular templates in a manner heretofore not possible," said Kiesnar. (outsourcing-pharma.com)
The combination of their miRNA tools with our knowledge of cancer drug mechanisms of action will allow the combined companies to identify new molecular diagnostic tests to improve patient response and survival. (outsourcing-pharma.com)
The use of Oncotech's extensive tumour bank and paraffin embedded samples will enable Exiqon to develop molecular diagnostic tests that test for altered miRNA expression levels in tumour samples. (outsourcing-pharma.com)
The company aims to have its first molecular diagnostic test based on miRNA biomarkers launched within the first 12 months following completion of the acquisition and is confident that the transaction will not compromise its financial goal of reaching profitability by 2011. (outsourcing-pharma.com)

Shock-frozen brain sections of six BSE-infected and five non-infected macaques were used to validate regulated miRNA candidates by two independent qRT-PCR-based methods. (biomedcentral.com)
Learn about methods for calculating oligonucleotide melting temperature (Tm). (sigmaaldrich.com)
invention also provides methods for selecting a preferred therapy for a particular subject or group of subjects or individuals at risk for or suffering from schizophrenia or psychosis by use of miRNAs. (genomeweb.com)
IDT has pioneered the use of high throughput Quality Control methods and is the only oligonucleotide manufacturer that offers 100% QC and purity guarantees. (mbiotech.co.kr)

Finally, the calibrated model is used to study the effect of different miRNA expression profiles and cooperative target regulation on p21 expression levels in different biological contexts. (hindawi.com)
However, experimental approaches have limitations when dealing with complex biological systems composed of multiple layers of regulation such as the transcriptional and post-transcriptional regulation by transcription factors (TFs) and miRNAs [ 2 ]. (hindawi.com)
Thus we hypothesized that miRNA regulation may also play a role in human prion diseases. (biomedcentral.com)

Inside https://www.selleck.co.jp/products/aspirin-acetylsalicylic-acid.html vivo photo indicated that the pin selleck chemicals llc fluorescence restoration with the press reporter probe from your ColoR9 MB improved gradually in the course of neuronal difference of P19 tissue, while crimson fluorescence task with the reference point probe remained consistent. (mglursignaling.com)
As a positive control for nucleic acid integrity in the tissue sections, we used a probe that hybridizes to a conserved region of the highly expressed PPIB of M. musculus (Cat. (cdc.gov)
The duplex oligonucleotide complexes of the disclosure include a conjugate moiety that facilitates delivery to a cell, tissue, or organism. (genomeweb.com)

Functional analyses of ectopically expressed copies of the variant miRNA precursors demonstrate loss of function, gain of function, and altered expression levels. (genomeweb.com)

Moreover, there is now ample evidence that perturbations in the levels of individual or entire families of miRNAs are strongly associated with the pathogenesis of a wide range of human diseases. (biomedcentral.com)

All Double-Dye Oligonucleotides are HPLC purified. (eurogentec.com)

No. 63/298,867, filed Jan. 12, 2022, entitled "CASCADE OLIGONUCLEOTIDE DISPLACEMENT PROBES," the entire contents of which are incorporated herein by reference. (justia.com)

The quantum sequencer identified m6A and 5mC in miRNAs extracted from colorectal cancer cells based on their conductance profiles obtained from the nanogap devices. (scienceboard.net)

Even so, the actual on/off-tunable miRNA MB is difficult to differentiate if the seen minimal fluorescence brightness is caused by lower miRNA expression as well as minimal transfection from the miRNA MB. (mglursignaling.com)

2021 ). For example, Hu reported that serum miRNA-21 was up-expressed as the T-stage osteosarcoma increased and demonstrated that miRNA-21 could inhibit the proliferation of osteosarcoma through targeting PTEN and regulating the TGF-1 signaling pathway (Hu et al. (springeropen.com)

The recent explosion in miRNA research has accelerated the development of several computational and experimental approaches for probing miRNA functions in cell culture and in vivo . (biomedcentral.com)

This includes patents and patent applications of IDT related to the right to use oligonucleotide probes labeled with Iowa Black ® FQ, Iowa Black RQ, TAO™, and/or ZEN™ quenchers. (idtdna.com)
In case of extra long Double-Dye Oligonucleotides probes are required, quenchers like Black Hole Quencher®, Deep Dark Quencher I and TAMRA can be coupled internally. (eurogentec.com)

Stability studies at IDT have shown that oligonucleotides stored in water at room temperature may show some degradation in as little as 60 days. (mbiotech.co.kr)
The same studies showed that oligonucleotides stored dry or in TE at room temperature did not show signs of degradation for at least 7 months. (mbiotech.co.kr)

Therefore, the introduction of a systematic approach, which can unravel the underlying mechanisms by which miRNAs exert their functions, becomes increasingly appealing. (hindawi.com)
Finally, the integration of quantitative data and modeling helped us to generate and validate hypotheses about mechanisms of collective miRNA repression on p21. (hindawi.com)

However, purification will always lower oligonucleotide final yield. (mbiotech.co.kr)

The simplest approach to MGB probe design is to use an MGB support, add a quencher molecule as the first addition and complete the synthesis with a 5'-fluorophore. (genelink.com)

Anatomy9

Organisms35

Diseases1

Chemicals and Drugs77

Analytical, Diagnostic and Therapeutic Techniques and Equipment48

Phenomena and Processes76

Disciplines and Occupations7

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture2

Information Science8

Health Care15

MicroRNAs15

Complementary11

Primers8

MicroRNA6

Hybridization12

Gene expression4

MRNA12

Analyze miRNA expression2

Detection9

RNAs1

Single-stranded3

Assays2

Mature miRNA1

MRNAs2

SiRNA1

Role of miRNAs1

Polymerase3

Double-stranded2

Therapeutics3

Genes7

Sequence3

Several miRNAs2

Multiple miRNAs2

QPCR probes page2

Targets5

Synthetic2

Abstract1

Locked nucl3

Amplification2

Detect1

Real-tim1

Nuclease probes1

Primary transcripts1

Tumor4

PrimeTime8

Fragments2

Antisense3

Regulatory4

Regulate1

TaqMan2

Expression patterns1

Fluorescent5

Molecules play1

Roles1

Differential expression1

Primer4

Molecular4

Methods4

Regulation3

Tissue3

Functional1

Pathogenesis1

HPLC1

20221

Colorectal1

Transfection1

Serum1

Experimental approaches1

Quenchers2

Degradation2

Mechanisms2

Purification1

Fluorophore1