Methylation of arginine residues is catalyzed by three different classes of protein argininemethyltransferases (PRMTs): Type I PRMTs (PRMT1, PRMT2, PRMT3, PRMT4, PRMT6, and PRMT8) attach two methyl groups to a single terminal nitrogen atom, producing asymmetric dimethylarginine (N G,N G-dimethylarginine). (wikipedia.org)
Immunoprecipitation2
High-throughput sequencing refers to the use of antibody immunoprecipitation or chemical methods to accurately locate the site of m7G modification in RNA. (beds.ac.uk)
STAG3 N6-methyladenosine (m6A) modification level were identified using m6A RNA immunoprecipitation (MeRIP). (bvsalud.org)
Protein7
Protein methylation is a type of post-translational modification featuring the addition of methyl groups to proteins. (wikipedia.org)
Although histones are the prime target of lysinemethyltransferases, other cellular proteins carry N-methyllysine residues, including elongation factor 1A and the calcium sensing protein calmodulin. (wikipedia.org)
Additionally, the functional roles of methyltransferase-like protein 3 (METTL3) and insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2) in CRC were explored by manipulating their levels via knockdown or overexpression. (bvsalud.org)
Finally, we discovered two kinds of strain-typing protein-encoding genes, i.e., the SAM-dependent methyltransferase and electron transport complex subunit RsxC. (bvsalud.org)
RNA methyltransferase nucleolar protein p120 (NOP2), commonly referred to as NOP2/Sun RNA methyltransferase family member 1 (NSUN1), is involved in cell proliferation and is highly expressed in various cancers. (bvsalud.org)
PRMTs1
In contrast, type II PRMTs (PRMT5 and PRMT9) catalyze the formation of symmetric dimethylarginine with one methyl group on each terminal nitrogen (symmetric N G,N' G-dimethylarginine). (wikipedia.org)
Epigenetics refers to heritable changes in gene expression without changing the nucleotide sequence of the gene, including DNA methylation, histone modifications, and RNA modifications. (beds.ac.uk)
In particular, certain types of ALKBH enzymes are dioxygenases that directly reverse DNA methylation damage via transfer of a methyl group from the DNA adduct onto α-ketoglutarate and release of metabolic products including succinate and formaldehyde. (frontiersin.org)
Functional roles1
With the accurate identification of m7G modifications within RNAs, their functional roles in the regulation of gene expression and different physiological functions have been revealed. (beds.ac.uk)
Most lysinemethyltransferases contain an evolutionarily conserved SET domain, which possesses S-adenosylmethionine-dependent methyltransferase activity, but are structurally distinct from other S-adenosylmethionine binding proteins. (wikipedia.org)
M7G modification, known as one of the common post-transcriptional modifications of RNA, is present in many different types of RNAs. (beds.ac.uk)
With the rapid development of sequencing technology, m7G has become a new research hotspot of RNA modification. (beds.ac.uk)
Residues1
In interactions of proteins with nucleic acids, arginine residues are important hydrogen bond donors for the phosphate backbone - many arginine-methylated proteins have been found to interact with DNA or RNA. (wikipedia.org)
Common1
Molecular formula of common RNA modifications. (beds.ac.uk)
Repeat1
Interestingly, both of these proteins carried an amino acid repeat in these strains: SAM-dependent methyltransferase comprised a single-amino-acid repeat (asparagine), whereas RsxC carried a 40-amino-acid repeat, which was differentially distributed among the strains. (bvsalud.org)
Polymerase3
This is required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination (PubMed:26700805). (assaygenie.com)
We present the first comprehensive analysis of RNA polymerase III (Pol III) transcribed genes in ten yeast genomes. (bvsalud.org)
Suppression of the ppk feature of heat sensitivity by extra copies of rpoS, the gene encoding the RNA polymerase sigma factor that regulates some 50 stationary-phase genes, further implicates poly P in promoting survival in the stationary phase. (nih.gov)
PRMT51
In contrast, type II PRMTs (PRMT5 and PRMT9) catalyze the formation of symmetric dimethylarginine with one methyl group on each terminal nitrogen (symmetric N G,N' G-dimethylarginine). (wikipedia.org)
Histone2
Methylates histone H2A and H4 'Arg-3' during germ cell development. (assaygenie.com)
Methylates histone H3 'Arg-8', which may repress transcription. (assaygenie.com)
Interproscan1
UvrD/REP helicase N-terminal domain, UvrD-like helicase C-terminal domain, DNA helicase IV / RNA helicase N terminal [Interproscan]. (ntu.edu.sg)
Proteins2
In interactions of proteins with nucleic acids, arginine residues are important hydrogen bond donors for the phosphate backbone - many arginine-methylated proteins have been found to interact with DNA or RNA. (wikipedia.org)
Most lysinemethyltransferases contain an evolutionarily conserved SET domain, which possesses S-adenosylmethionine-dependent methyltransferase activity, but are structurally distinct from other S-adenosylmethionine binding proteins. (wikipedia.org)
Metabolic channeling offers unique opportunities for enhancing and regulating cellular biochemistry and major advantage of such spatial organization is the transfer of biosynthetic intermediates between catalytic sites without diffusion into the bulk phase of the cell [14]. (scirp.org)