Methods oligonucleotide array sequence analysis. Medical search. Frequent questions

The core principle behind microarrays is hybridization between two DNA strands, the property of complementary nucleic acid sequences to specifically pair with each other by forming hydrogen bonds between complementary nucleotide base pairs. (wikipedia.org)
Microarrays can be fabricated using a variety of technologies, including printing with fine-pointed pins onto glass slides, photolithography using pre-made masks, photolithography using dynamic micromirror devices, ink-jet printing, or electrochemistry on microelectrode arrays. (wikipedia.org)
In spotted microarrays, the probes are oligonucleotides, cDNA or small fragments of PCR products that correspond to mRNAs. (wikipedia.org)
Massively parallel experimental assays, particularly DNA microarrays and sequencing, have revolutionized genomics research in the last decades. (nature.com)
The results microarrays represent a promising new technology that has demonstrate a novel, timely, and unbiased method for the been used to rapidly and accurately identify nucleotide molecular epidemiologic surveillance of influenza viruses. (cdc.gov)
Use of resequencing microarrays to Influenza viruses are a major cause of respiratory infec- detect single nucleotide polymorphisms and generate pri- tions in humans and result in substantial illness, death, mary sequences enables identification of genetic variants and economic problems throughout the world. (cdc.gov)
In what follows we limit ourselves to the analysis of gene expression measurements obtained using microarrays with small spots of DNA fixed to glass slides. (lu.se)
article{, title = {Microarrays as cancer keys: an array of possibilities}, author = { S. Mohr and G. D. Leikauf and G. Keith and B. H. Rihn}, year = {2002}, date = {2002-01-01}, journal = {J Clin Oncol}, volume = {20}, number = {14}, pages = {3165-75}, abstract = {Malignant transformation results from accumulation of genetic and epigenetic events. (cnrs.fr)
Several technologies exist to evaluate these changes including microarrays and serial analysis of gene expression (Sage). (chemistryworld.com)
DNA microarrays take advantage of the major feature of the DNA double helix, the sequence complementarity of the two-paired strands, by using DNA capture probes which are the complement of the expressed target sequence (mRNA, cRNA or cDNA made from the mRNA). (ddw-online.com)
Two of the most common uses of the DNA microarrays are genetic analysis and the analysis of gene expression. (ddw-online.com)
This report will focus in using DNA microarrays for the analysis of gene expression or expression profiling, and how DNA chips are used for drug discovery and development. (ddw-online.com)
High-density oligonucleotide microarrays were used to perform experiments for comparing data generated by using two protocols, an in vitro transcription (IVT) protocol that requires 5 μg of total RNA and a double in vitro transcription (dIVT) protocol that requires 200 ng of total RNA for target preparation from RNA samples extracted from a normal and a cancer cell line. (biomedcentral.com)
Gene expression profiles from 128 adult patients with newly diagnosed ALL were characterized using high-density oligonucleotide microarrays. (aacrjournals.org)
DNA microarrays are simply platforms that consist of small solid supports onto which the sequences from thousands of different genes are attached at fixed locations. (medscape.com)
The 2 most common microarray technologies in use are the oligonucleotide microarrays and the robotically spotted complementary DNA (cDNA) microarrays. (medscape.com)
Oligonucleotide microarrays are manufactured by Affymetrix (Santa Clara, Calif) using photolithographic techniques. (medscape.com)

Data from whole transcriptome sequencing of 33 breast specimens in the Harbin Medical University Cancer Center cohort and The Cancer Genome Atlas was applied to identify and validate the landscape of tumor suppressor long noncoding RNAs, which was further validated by The Cancer Genome Atlas pancancer data including 33 cancer types and 12,839 patients. (cancerindex.org)
The variety of instrumentation allows for capabilities ranging from highly focused analysis of candidate SNPs and mRNAs to whole genome, exome, epigenome and transcriptome sequencing. (psu.edu)
The expression of candidate genes was evaluated by RT-PCR in a Cuban dengue patient cohort and gene set enrichment analysis was performed in a Thai dengue transcriptome. (hal.science)
Furthermore, single-cell transcriptome sequencing was used to elucidate the cell type composition of the cervix sample and analyze the expression levels of key genes in cells. (researchsquare.com)
Scholars@Duke publication: Analysis of methylation-sensitive transcriptome identifies GADD45a as a frequently methylated gene in breast cancer. (duke.edu)

La determinación de un patrón de genes expresados al nivel de la TRANSCRIPCIÓN GENÉTICA bajo circunstancias específicas o en una célula específica. (bvsalud.org)
Furthermore, methods to rank the genes according to their importance for the classification are explored. (lu.se)
As the sequencing and gene annotation projects of entire genomes of many species are headed towards completion (see, e.g. [13] ), massive mapping efforts in biology are now focused on how the genes interact. (lu.se)
The recent advance in the microarray data analysis makes it easy to simultaneously measure the expression levels of several thousand genes. (hindawi.com)
In order to evaluate the proposed method, we tested MWIS-ACO-LS on ten well-replicated microarray datasets of high dimensions varying from 2308 to 12600 genes. (hindawi.com)
Thus, extracting a small subset of genes containing valuable information about a given cancer is one of the principal challenges in the microarray data analysis [ 4 ]. (hindawi.com)
In wrapper methods, the selection can be seen as an exploration of all the possible subsets, and the principle is to generate a subset of genes and evaluate it afterward. (hindawi.com)
The completion of the sequencing of the human genome has uncovered 32,000 to 35,000 predicted genes, offering the prospect for many more drugable targets. (ddw-online.com)
The sequence analysis of the 32-35,000 predicted genes indicates the there may be as many as 1,000 kinase or kinase-like genes. (ddw-online.com)
Analysis of gene expression can include measuring expression levels of a small set of genes to whole genome expression monitoring. (ddw-online.com)
This method was used to measure the number of copies of repeated genes such as rRNA genes (2) and to measure whether specific genes were under-replicated during the replication process used in forming polytene chromosomes (3). (ddw-online.com)
Global ancestry analysis based on high-throughput genotyping in admixed populations can be used to test this hypothesis, while admixture mapping can map candidate protective genes. (hal.science)
We hope to provide a concise review of the literature and genes involved within our deletion sequence in the hope that it will contribute to creating a phenotypic profile for this patient population. (hindawi.com)
Genes were filtered to exclude those that were undetected on all arrays. (biomedcentral.com)
Gene coexpression analysis refers to the en masse discovery of coexpressed genes from a large variety of transcriptomic experiments. (mdpi.com)
Supervised analysis of kinase genes revealed a high-level FLT3 expression in a subset of cases without molecular rearrangements. (aacrjournals.org)
Northern blotting for five of these genes (alpha-Catenin, DTR, FYN, GADD45a, and Zyxin) verified the array results. (duke.edu)
Further analysis of one of these genes, GADD45a, showed that 5-AzaC induced expression in five additional breast cancer cell lines with little or no induction in three additional lines derived from normal breast epithelial cells. (duke.edu)
Therefore, this simple method of dynamic transcriptional profiling yielded a series of novel methylation-sensitive genes in breast cancer including the BRCA1 and p53 responsive gene, GADD45a. (duke.edu)
The physiological conditions experienced by a microbial community can thus be inferred using meta-transcriptomic sequencing by comparing transcription levels of specifically chosen genes. (osti.gov)
However, this analysis requires accurate reference genomes to identify the specific genes from which RNA reads originate. (osti.gov)
Sample-specific meta-genomic assembled genomes (MAGs) were used as reference genomes to accurately identify the origin of RNA reads, and transcript ratios of genes with opposite transcription responses were compared to eliminate biases related to differences in organismal abundance, an approach hereafter named the "diametric ratio" method. (osti.gov)
The introduction of complementary DNA (cDNA) microarray technology in 1995 has helped to facilitate the identification and classification of DNA sequence information and the assignment of functions to these new genes by allowing investigators to analyze expression of thousands of genes simultaneously in a single experiment. (medscape.com)

The process of measuring gene expression via cDNA is called expression analysis or expression profiling. (wikipedia.org)
The use of splice-junction oligonucleotides to analyze splice events was proposed as early as 1986, when Morgan and Ward used them to identify differential splice forms of minute virus in mice cDNA [ 14 ]. (biomedcentral.com)
In the IVT protocol, 5-40 μg of total RNA is reverse-transcribed to generate cDNA using an oligo-dT primer containing the T7 promoter sequence. (biomedcentral.com)
METHODS: Low-density assays (cDNA microarray and microfluidics qPCR) were designed, based on the results of the high-density microarray data. (ox.ac.uk)
This cDNA can then be fluorescently labeled and hybridized to the target gene sequences on the microarray. (medscape.com)
Despite its biological importance, transfer RNA (tRNA) could not be adequately sequenced by Author Manuscript standard methods due to abundant post-transcriptional modifications and stable structure, which interfere with cDNA synthesis. (cdc.gov)
Widely used RNA-seq methods start with adapter ligation and cDNA synthesis of biological RNA samples followed by PCR amplification to generate sequencing libraries1. (cdc.gov)
Significant obstacles for the sequencing of tRNA include the presence of numerous post-transcriptional modifications and its stable and extensive secondary structure, which interfere with cDNA synthesis and adapter ligation. (cdc.gov)

Probe-target hybridization is usually detected and quantified by detection of fluorophore-, silver-, or chemiluminescence-labeled targets to determine relative abundance of nucleic acid sequences in the target. (wikipedia.org)
The original nucleic acid arrays were macro arrays approximately 9 cm × 12 cm and the first computerized image based analysis was published in 1981. (wikipedia.org)
Disclosed are materials and methods for performing multiplex assays for nucleic acids, in which a transponder is associated with the bead(s) forming the solid phase used in the assay, nucleic acid probes are bound to the surface of the particles, and data concerning the assay is encoded on the transponder. (justia.com)
This invention relates to materials and methods for detecting nucleic acids in samples and, more particularly, to solid phase assays wherein transponders are associated with the beads constituting the solid phase, nucleic acid probes are bound to the surface of the particles and data concerning the assay is encoded on the transponders. (justia.com)
In an electronically-indexed multiplex assay of this invention, two or more classes of transponders, each encoded with a different index number and constructed to bind a different nucleic acid sequence, are incubated with the sample in a single vessel. (justia.com)
After necessary washes, incubations and additions are performed, the solid phase is analyzed to detect a label indicative of binding of nucleic acid in the sample to the oligonucleotide on the transponder, such as fluorescence, color, radioactivity or the like. (justia.com)
Early studies of DNA melting and reformation were carried out in aqueous solutions and yielded important information about the dependence of melting temperature (Tm) on the G+C composition and salt concentration, as well as information on the dependence of the rate of reassociation on the sequence complexity of the nucleic acid. (ddw-online.com)
The introduction of solid supports for DNA hybridisation/reassociations greatly broadened the range of applications of nucleic acid hybridisations, and provided the basis for solid-based methods being used today. (ddw-online.com)

Integrative analysis of sequencing and array genotype data for discovering disease associations with rare mutations. (unc.edu)
Methodology: This was a hospital-based, analytical cross-sectional study carried out on 226 symptomatic women wherein cervico-vaginal samples were obtained during gynaecological examination for Pap smears, HPV-DNA and genotype detection with linear array HPV strip, conducted from November 2019 to January 2021. (bvsalud.org)

The patent provides the means for producing libraries of peptide structures for drug screening applications that are capable of folding or assuming their native conformations independently of artificial scaffolds or flanking sequences in the proteins from which they are derived. (genomeweb.com)

High-throughput sequencing-RNA affinity profiling (HiTS-RAP) 22 is conceptually similar to RNA-MaP, but uses the E. coli replication terminator protein Tus to stall the RNA polymerase after transcribing the variable section of the DNA library. (nature.com)
This method is applicable to correct the library biases in various studies that use highly multiplexed sequencing-based profiling methods with a consistent sample layout with samples to be compared (e.g., "cases" and "controls") equally distributed in each library. (biomedcentral.com)
RNA sequencing (RNAseq) utilizing next generation sequencers has allowed for rapid and comprehensive expression profiling. (biomedcentral.com)

Genetic analysis includes procedures for genotyping, SNP (single nucleotide polymorphism) detection, strain identification and various other procedures. (ddw-online.com)

Each DNA spot contains picomoles (10−12 moles) of a specific DNA sequence, known as probes (or reporters or oligos). (wikipedia.org)
Many types of arrays exist and the broadest distinction is whether they are spatially arranged on a surface or on coded beads: The traditional solid-phase array is a collection of orderly microscopic "spots", called features, each with thousands of identical and specific probes attached to a solid surface, such as glass, plastic or silicon biochip (commonly known as a genome chip, DNA chip or gene array). (wikipedia.org)
Arrays from commercial vendors may have as few as 10 probes or as many as 5 million or more micrometre-scale probes. (wikipedia.org)
The probes are synthesized prior to deposition on the array surface and are then "spotted" onto glass. (wikipedia.org)
A common approach utilizes an array of fine pins or needles controlled by a robotic arm that is dipped into wells containing DNA probes and then depositing each probe at designated locations on the array surface. (wikipedia.org)
These arrays may be easily customized for each experiment, because researchers can choose the probes and printing locations on the arrays, synthesize the probes in their own lab (or collaborating facility), and spot the arrays. (wikipedia.org)
The array consisted of exon probes and thermodynamically balanced junction probes. (biomedcentral.com)
Suboptimal probes were tagged and considered in the final analysis. (biomedcentral.com)
These arrays contain oligonucleotide probes that span exon-exon junctions, and probes positioned within exons to determine individual exon levels and overall transcript expression. (biomedcentral.com)
The cross-linked sample DNA was hybridized with sequence-specific oligonucleotide probes to identify HLA-B27 allele sequences. (cdc.gov)
A microarray containing the probes, and a kit containing the array and the HLA gene-specific primers, is also claimed. (genomeweb.com)
This set of tracks shows the genomic positions of probes and targets from a full suite of in-solution-capture target enrichment exome kits for Next Generation Sequencing (NGS) applications. (ucsc.edu)

Array comparative genomic hybridization (aCGH) enables the detection of CNVs of single and multiple exons within a given gene (Tayeh et al. (preventiongenetics.com)
The discipline of obtaining information about genomic or protein sequence data. (aporc.org)
Genomic and functional analysis of emerging virulent and multi-drug resistant E. coli lineage ST648. (cdc.gov)

Fluorescently labeled target sequences that bind to a probe sequence generate a signal that depends on the hybridization conditions (such as temperature), and washing after hybridization. (wikipedia.org)
In most cases, however, circulating strains, novel strains emerge sporadically this technology has relied on specific amplification of a because of reassortment in the segmented influenza RNA limited number of target sequences before hybridization, genome and have resulted in devastating influenza pan- thus restricting throughput and limiting final identification demics ( 1-3 ). (cdc.gov)

Extended insight into the Mycobacterium chelonae-abscessus complex through whole genome sequencing of Mycobacterium salmoniphilum outbreak and Mycobacterium salmoniphilum-like strains. (cdc.gov)

[ 7 ] They consist of a glass surface onto which oligonucleotides consisting of 25 bases are built a single nucleotide at a time. (medscape.com)

A high number of complementary base pairs in a nucleotide sequence means tighter non-covalent bonding between the two strands. (wikipedia.org)
The index number may define the nucleotide sequence of the oligonucleotide deposited on the surface of the particle, the catalog number of a DNA fragment deposited on the particle, index numbers of chemical steps which were involved in the chemical synthesis of an oligonucleotide bound to the particle, or some other relevant characteristics of the deposited molecules. (justia.com)

Quantitative analysis of RNA on a massively parallel array (RNA-MaP) 21 , 23 starts with ssDNA sequencing libraries including an RNA polymerase promoter region, a stall sequence, and a variable region complementary to the desired RNA. (nature.com)
1996). In cases where the majority of the reported pathogenic variant in a gene can be detected by DNA sequencing, PreventionGenetics' aCGH is an excellent complementary test when DNA sequencing fails to identify the causative pathogenic variant(s). (preventiongenetics.com)

Nevertheless, together with benchmarking analyses, we show here that the diametric ratio approach can be applied for evaluating the physiological conditions experienced by microbes in situ. (osti.gov)

In an attempt to adapt resequencing microarray technol- ceptibility, and viral escape from vaccine-elicited immu- ogy to surveillance and diagnostics, we developed the res- nity, continually surveying the genetic composition (i.e., piratory pathogen microarray (RPM) version 1 for primary sequence) of circulating and emerging variants is detection and sequence typing of 20 common respiratory necessary. (cdc.gov)
Single-cell genetic analysis of clonal dynamics in colorectal adenomas indicates CDX2 gain as a predictor of recurrence. (cancerindex.org)

However, given the mass of data emerging from this analysis, and commonalities they reveal between various phenomena/disorders, achieving 100% certainty may not be that easy. (medscimonit.com)
Computational Statistics and Data Analysis. (nycu.edu.tw)
In addition to iden- sequencing produces accurate data, the requirement for tification, this method provided primary sequence informa- knowledge of template sequences and the inability to tion, which suggested that distinct lineages of influenza quickly process multiple targets hinder its practical appli- viruses co-circulated during the 2004-2005 season, and cation in epidemiologic and diagnostic investigations. (cdc.gov)
Various supervised and unsupervised data mining methods for analyzing the produced high- dimensional data are discussed. (lu.se)
Typically when data are presented, the fluor- escent intensity from the tissue is pseudo-colored red and the intensity from the reference green, and the logarithmic ratio of background corrected red and green intensities for each gene (spot) is subject to analysis (see Fig. 1 ). (lu.se)
In this work, we are interested in gene expression data dimension reduction for cancer classification, which is a common task in most microarray data analysis studies. (hindawi.com)
The analysis of NHANES laboratory data must be conducted using the key survey design variables and basic demographic variables. (cdc.gov)
A method of processing data from the reading of biopolymer arrays is also provided. (genomeweb.com)
The data generated by using these methods must be extensively evaluated to determine any technique dependent distortion of the expression profiles. (biomedcentral.com)
It is vital to be able to distinguish between the real effects of the biological system being analyzed and changes introduced due to a difference in the methods used to generate the data. (biomedcentral.com)
PGxome and exome-based sequencing panels, including custom panels, include copy number variant (CNV) analysis via NextGen sequencing (NGS) data at no additional cost. (preventiongenetics.com)
We present NicheNet ( https://github.com/saeyslab/nichenetr ), a method that predicts ligand-target links between interacting cells by combining their expression data with prior knowledge on signaling and gene regulatory networks. (nature.com)
In addition, we provide tutorials on model validation (evaluation of target gene and ligand activity), model construction and parameter optimization such that users can benchmark their methods against NicheNet and construct their own models (for example, with their own data sources to include more cell type specificity). (nature.com)
The scripts used for processing of data sources and to carry out all analyses from this study are also available at Zenodo ( https://doi.org/10.5281/zenodo.3462199 ). (nature.com)
A method for testing how well a particular data set fits a model. (aporc.org)
Because of the complexity of the data sets generated by microarray experiments, the use of data-analysis software is essential. (medscape.com)
Several commercial and public data-analysis tools have been developed for this purpose. (medscape.com)
Building the framework for standardized clinical laboratory reporting of next generation sequencing data for resistance-associated mutations in Mycobacterium tuberculosis complex. (cdc.gov)
The GEO accession number for the sequencing data reported in this paper is GSE66550. (cdc.gov)

To generate specific transcript profiles, one must isolate homogenous cell populations using techniques that often yield small amounts of RNA, requiring researchers to employ RNA amplification methods. (biomedcentral.com)
In such cases, it is often necessary to employ RNA amplification methods to generate the microgram quantities of RNA required to perform these experiments. (biomedcentral.com)
The use of RNA amplification methods warrants a thorough analysis and understanding of the variations introduced due to the methodology employed. (biomedcentral.com)

Cultures testing positive for Each tiled prototype sequence was selected to have an influenza A or B viruses were confirmed by using reverse intermediate level of sequence homology across a group of transcription-polymerase chain reaction (RT-PCR) analy- microbial or viral strains, which allowed for efficient sis with previously reported protocols ( 16,17 ). (cdc.gov)
HLA-B27 testing was performed on these samples using a polymerase chain reaction (PCR) to specifically replicate the DNA sequences encoding the HLA loci of interest. (cdc.gov)

Methods are claimed that enable the dual end-tagging of RNA to prepare libraries for analysis by applications such as next-generation RNA sequencing, qPCR, microarray analysis, or cloning. (genomeweb.com)

The CpG island associated with GADD45a was analysed by bisulfite sequencing, sampling over 100 CpG dinucleotides. (duke.edu)

The activated phosphoramidite in 1.5 - 20-fold excess over the support-bound material is then brought in contact with the starting solid support (first coupling) or a support-bound oligonucleotide precursor (following couplings) whose 5′-hydroxy group reacts with the activated phosphoramidite moiety of the incoming nucleoside phosphoramidite to form a phosphite triester linkage. (3dresearchspecs.com)

An example of its application is in SNPs arrays for polymorphisms in cardiovascular diseases, cancer, pathogens and GWAS analysis. (wikipedia.org)
The ethics of sequencing infectious disease pathogens for clinical and public health. (cdc.gov)

Since mutations and reassortments are often to strains that retain primer-targeted sequences. (cdc.gov)
In this chapter, we describe the utility of a microarray-based approach exploiting short overlapping oligonucleotides (sliding-frame array) to rapidly detect drug resistance-associated mutations (substitutions, deletions, and insertions) in the pncA gene responsible for resistance ofM. (johnshopkins.edu)

In the absence of quantitative predictive capacity for such bioaffinity interactions, high throughput experimental approaches are needed to sufficiently sample RNA sequence space. (nature.com)
The scanner that records the intensity value is linked to digital image analysis software, which produces a color-coded image of the array, and a quantitative value is recorded for each target gene. (medscape.com)
The lack of efficient and quantitative tRNA-seq methods has hindered biological studies of tRNA. (cdc.gov)
We applied two strategies to eliminate or substantially reduce the obstacles of tRNA modification and structure for efficient and quantitative tRNA sequencing ( Fig. 1a ). (cdc.gov)

After the DNA library is sequenced on the Illumina flow cell, it is enzymatically double stranded by extension of a 5′ biotinylated primer. (nature.com)

Pmage is a technique that takes the best of Sage and high throughput DNA sequencing to obtain a global distribution of mRNA in any cell or tissue for a cost of about $1500 (?757) compared to about $10 000 for a Sage analysis, explained Seidman. (chemistryworld.com)
Now we have a method that gives a very clear frequency distribution of mRNA molecules in a cell,' he said. (chemistryworld.com)

In a more recent, more convenient, and more widely used method, the synthesis starts with the universal support where a non-nucleosidic linker is attached to the solid support material (compounds 1 and 2). (3dresearchspecs.com)
The method, initially developed for the solution-phase synthesis, was also implemented on low-cross-linked "popcorn" polystyrene,[26] and later on controlled pore glass (CPG, see "Solid support material" below), which initiated a massive research effort in solid-phase synthesis of oligonucleotides and eventually led to the automation of the oligonucleotide chain assembly. (3dresearchspecs.com)
More recently, high-throughput oligonucleotide synthesis where the solid support is contained in the wells of multi-well plates (most often, 96 or 384 wells per plate) became a method of choice for parallel synthesis of oligonucleotides on small scale. (3dresearchspecs.com)
Solid supports[edit] In solid-phase synthesis, an oligonucleotide being assembled is covalently bound, via its 3′-terminal hydroxy group, to a solid support material and remains attached to it over the entire course of the chain assembly. (3dresearchspecs.com)
Solid support material[edit] In contrast to organic solid-phase synthesis and peptide synthesis, the synthesis of oligonucleotides proceeds best on non-swellable or low-swellable solid supports. (3dresearchspecs.com)
The absence of physical dividers between the sites occupied by individual oligonucleotides, a very limited space on the surface of the microarray (one oligonucleotide sequence occupies a square 25×25 μm)[107] and the requirement of high fidelity of oligonucleotide synthesis dictate the use of site-selective 5′-deprotection techniques. (3dresearchspecs.com)
Synthesis of oligonucleotides by the H-Phosphonate Method Thirty years later, this work inspired, independently, two research groups to adopt the H-phosphonate chemistry to the solid-phase synthesis using nucleoside H-phosphonate monoesters 7 as building blocks and pivaloyl chloride, 2,4,6-triisopropylbenzenesulfonyl chloride (TPS-Cl), and other compounds as activators. (3dresearchspecs.com)

Patients with autosomal dominant disorders with no pathogenic variant identified by DNA sequencing. (preventiongenetics.com)
Patients with autosomal recessive disorders with one or no pathogenic variants identified by DNA sequencing. (preventiongenetics.com)
Male patients with X-linked disorders with no pathogenic variant identified by DNA sequencing or PCR fails. (preventiongenetics.com)
Female patients with X-linked disorders with one or no pathogenic variants have been identified by DNA sequencing. (preventiongenetics.com)
justifiant de ce fait une meilleure prise en charge de ces patients. (bvsalud.org)
La présente étude détermine la prévalence de l'infection par le virus de l'hépatite C en en determinant les génotypes ainsi que les facteurs y associés dans ce groupe de patients. (bvsalud.org)

We conduct sequence read alignment, secondary analysis (quantitation, variant calling, functional annotation, visualization, etc) and follow-up with the interpretation of the results. (psu.edu)

Enzo Life Sciences of Farmingdale, NY, has received US Patent No. 8,574,881, "Compositions useful for detection or quantification of desirable target molecules, novel dyes, composite dyes, and oligonucleotides or polynucleotides comprising such dyes. (genomeweb.com)
For more information about CNV detection via NGS, please refer to the specific sequencing test descriptions of interest on our website. (preventiongenetics.com)
A new oligonucleotide array for the detection of multidrug and extensively drug-resistance tuberculosis. (cdc.gov)

Allows for insertions or deletions in the sequences being aligned. (aporc.org)

Exons 2 and 3 of the HLA-B locus were amplified with locus specific primers and the amplified DNA was arrayed onto seven replicate nylon membranes and immobilized by UV cross-linking. (cdc.gov)
In addition, 300 bp of flanking intronic sequence on either side of targeted exons has enriched probe coverage. (preventiongenetics.com)

developed the first microarray containing splice-junction oligonucleotides to analyze splice events in yeast [ 16 ]. (biomedcentral.com)
METHODS: A multicenter observational study was conducted to analyze serotype 3 isolates collected between 2012 and 2021. (bvsalud.org)

Promoter arrays are also very common, they typically cover all refeq promoters of the species (human, mouse or rat) of interest. (epigenie.com)

DNA methylation arrays are the definition of workhorses: they may be older, but they are tried and true and still get the job done. (epigenie.com)
Methylation arrays are designed to work with other techniques. (epigenie.com)
Methylation arrays are still very much is use to compliment or kick-off larger projects or perform large-scale, high throughput experiments. (epigenie.com)
Methylation-specific oligonucleotide microarray: A new potential for high-throughput methylation analysis. (epigenie.com)

In general, gene selection methods are divided into two subclasses: wrapper approaches and filter approaches [ 5 ]. (hindawi.com)
This method shares many of the advantages of Sage over hybridisation-based approaches [such as DNA chips] but is a significant improvement over Sage,' said Kenneth Kinzler, professor of oncology and director of the Ludwig Center at Johns Hopkins University in Baltimore, MD. 'This may become the gold standard for gene expression analyses,' he said. (chemistryworld.com)

But these methods are limited by their inability to detect low abundance RNAs. (chemistryworld.com)

After the completion of the coupling reaction, a small percentage of the solid support-bound 5′-OH groups (0.1 to 1%) remains unreacted and needs to be permanently blocked from further chain elongation to prevent the formation of oligonucleotides with an internal base deletion commonly referred to as (n-1) shortmers. (3dresearchspecs.com)
30] Once a phosphoramidite has been coupled to the solid support-bound oligonucleotide and the phosphite moieties have been converted to the P(V) species, the presence of the phosphate protection is not mandatory for the successful conducting of further coupling reactions. (3dresearchspecs.com)
Step 3: Capping[edit] The capping step is performed by treating the solid support-bound material with a mixture of acetic anhydride and 1-methylimidazole or, less often, DMAP as catalysts and, in the phosphoramidite method, serves two purposes. (3dresearchspecs.com)

Antimicrobial susceptibility testing and multilocus sequence typing were performed. (bvsalud.org)
Developing a more effective vaccine against serotype 3 and monitoring the antimicrobial-resistant sequence types are necessary. (bvsalud.org)
Serotyping, multi-locus sequence typing, and antimicrobial susceptibility testing for penicillin and ceftriaxone were performed. (bvsalud.org)
Sequencing-based methods and resources to study antimicrobial resistance. (cdc.gov)

The alternative bead array is a collection of microscopic polystyrene beads, each with a specific probe and a ratio of two or more dyes, which do not interfere with the fluorescent dyes used on the target sequence. (wikipedia.org)
The oligonucleotide positive control dots were required to be positive for each probe and to be clearly distinguishable from all negative controls. (cdc.gov)

We investigated 166 technical replicates in 14 RNAseq libraries made using the STRT method. (biomedcentral.com)
After sequencing and preprocessing, we sometimes observe a library bias - for example, reference samples in two different libraries may cluster apart by the libraries. (biomedcentral.com)

The analysis included a total of 21,729 HCWs and recorded 3,672 infections with no severe disease nor death. (bvsalud.org)
Methods The study of culture-confirmed pneumococcal disease (CCPD) was conducted at four hospitals across Taiwan in 2015-2018. (bvsalud.org)
Reconstructing foot-and-mouth disease outbreaks: a methods comparison of transmission network models. (cdc.gov)

As simultaneously identified and typed all component strains an alterative, high-density oligonucleotide resequencing of the trivalent FluMist intranasal vaccine. (cdc.gov)

On the substructure controls in rare variant analysis: Principal components or variance components? (unc.edu)

Anatomy5

Organisms4

Diseases1

Chemicals and Drugs37

Analytical, Diagnostic and Therapeutic Techniques and Equipment33

Phenomena and Processes64

Disciplines and Occupations3

Technology, Industry, Agriculture2

Information Science9

Health Care6

Microarrays17

Transcriptome5

Genes23

CDNA8

Nucleic acid8

Genotype2

Proteins1

Profiling3

Polymorphism1

Probes12

Genomic3

Hybridization2

Whole genome1

Single Nucleotide1

Nucleotide sequence2

Complementary2

Situ1

Genetic2

Data19

Amplification3

Polymerase2

QPCR1

Bisulfite1

Precursor1

Pathogens2

Mutations2

Quantitative4

Illumina1

MRNA2

Synthesis7

Patients6

Annotation1

Detection3

Deletions1

Exons2

Analyze2

Promoter1

Methylation4

Approaches2

RNAs1

Bound3

Antimicrobial4

Probe2

Libraries2

Disease3

Strains1

Variant1