• To compare sperm retrieval rates between unilateral and bilateral microdissection testicular sperm extraction (MD-TESE) procedures in patients with non-obstructive azoospermia and to contribute to the literature by comparing them with literature data. (springermedicine.com)
  • Bernie AM, Ramasamy R, Schlegel PN (2013) Predictive factors of successful microdissection testicular sperm extraction. (springermedicine.com)
  • Ichioka K, Matsui Y, Terada N, Negoro H, Goto T, Ogawa O (2020) Three-dimensional simulation analysis of microdissection testicular sperm extraction for patients with non-obstructive azoospermia. (springermedicine.com)
  • Microdissection testicular sperm extraction (micro-TESE) in men with infertility due to nonobstructive azoospermia: summary of current literature. (nih.gov)
  • Laser capture microdissection (LCM), also called microdissection, laser microdissection (LMD), or laser-assisted microdissection (LMD or LAM), is a method for isolating specific cells of interest from microscopic regions of tissue/cells/organisms (dissection on a microscopic scale with the help of a laser). (wikipedia.org)
  • Laser-capture microdissection (LCM) is a method to procure subpopulations of tissue cells under direct microscopic visualization. (wikipedia.org)
  • IR laser provides a more gentle approach to microdissection. (wikipedia.org)
  • Micro-sectioning coupled with laser capture microdissection (LCM) offers an opportunity for the isolation of feeding sites from heterogeneous cell populations. (biomedcentral.com)
  • Fluidigm has launched the AccuLift Laser Capture Microdissection System, which enables precise and efficient capture of individual cells or larger tissue regions from frozen and FFPE tissue sections for DNA, RNA, and protein biomarker analysis using sequencing, real-time PCR, or mass spectrometry. (genomeweb.com)
  • NCEZID scientists used a technique known as laser microdissection to pinpoint the specific cells where Zika virus might be persisting and replicating . (cdc.gov)
  • En moins d'une semaine, les cerveaux d'image utilisant un microscope confocal laser-balayage avec des lasers d'excitation et des cubes de filtre appropriĆ©s aux anticorps secondaires fluorescents choisis. (jove.com)
  • Another method called Geo-seq combines scRNA-seq with laser capture microdissection. (genomeweb.com)
  • Dr. Lubman's team used a combination of immunostaining with laser capture microdissection (immuno-LCM). (genengnews.com)
  • Instead, we used laser microdissection to excise putative progenitor cells and control cells from their in situ locations in cryosections and characterized the molecular properties of these cells. (frontiersin.org)
  • To study epigenetic changes and genes that are expressed differently in different cell lineages-such as cancer cells versus normal cells, or the two types of epidermal cells in Arabidopsi s roots-typically requires laser capture microdissection (LCM) or fluorescence-activated cell sorting (FACS). (the-scientist.com)
  • Amyloid plaques and neighbouring non-plaque tissue were microdissected from human brain sections using laser capture microdissection and label-free LC-MS was used to quantify the proteins present. (researchsquare.com)
  • Single-cell Deep Visual Proteomics integrates imaging, cell segmentation, laser microdissection and multiplexed mass spectrometry for spatial single-cell proteomics measurements in complex tissues. (nature.com)
  • The laser capture microdissection market is witnessing growth in the academic and research centers segment due to the increased funds allocated by government agencies to promote research in fields such as neurology, oncology, and proteomics. (technavio.com)
  • The laser capture microdissection market will continue to grow in the Americas throughout the predicted period. (technavio.com)
  • Alternatively, gene expression patterns can be resolved using laser capture microdissection (LCM), which samples tissues on a grid. (scienceboard.net)
  • The kit contains reagents for the effective cell lysis of isolated single cells or small clusters of cells, for example from laser-capture microdissection platforms. (qiagen.com)
  • Neurone, astrocyte, and endothelial cell-enriched mRNA, obtained by immuno-laser capture microdissection of temporal cortex (Brodmann area 21/22) from 6 cases with self-reported T2D in the Cognitive Function and Ageing Study neuropathology cohort, and an equal number of age and sex-matched controls, was assessed by microarray analysis. (biomedcentral.com)
  • Then as a clinical lecturer in Liverpool while training in paediatric infectious diseases his research took a more basic approach, to understand the mechanisms of his PhD observations: developing in vitro methods and using laser capture microdissection to examine specific cell populations using transcriptomics in patient samples. (gla.ac.uk)
  • Falk Schlaudraff (Institute of General Physiology of the University of Ulm, Germany) used modern laser microdissection methods to isolate and analyze cells from post mortem tissue specimens taken from M. Parkinson patients in order to gain molecular insight into the disease. (leica-microsystems.com)
  • As part of his Ph.D. he analyzed the gene expression of human dopaminergic neurons, which he isolated from post mortem midbrain tissue of Morbus Parkinson patients and control subjects using a laser microdissection system from Leica Microsystems. (leica-microsystems.com)
  • Our analysis, for example the RNA quality of the specimens, showed that the quality of the results is not influenced by the staining process and laser microdissection. (leica-microsystems.com)
  • A) Left panel: cresylviolet (CV)-stained mouse coronal midbrain section indicating the area of the substantia nigra pars compacta (SNpc) before (left) and after (right) UV -laser-microdissection of the entire area. (leica-microsystems.com)
  • Here, we describe a set of hierarchical analytical methods to process, combine, and harmonize single-cell, single-nucleus and subsegmental laser microdissection (LMD) transcriptomics with LMD and near single-cell proteomics, 3-D nondestructive and immunofluorescence-based Codex imaging and spatial metabolomics datasets. (biorxiv.org)
  • We collected 2000 individual cells per batch from FFPE liver slices with laser capture microdissection and used them as test samples. (bvsalud.org)
  • Laser microdissection microscopy has become a rising tool to assess gene expression profiles of pure cell populations. (biomedcentral.com)
  • [ 11-13 ] By contrast, we discovered, using laser capture microdissection and mass spectrometry, that keratin 17 (K17) could predict the overall survival of patients with cervical squamous cell carcinoma more accurately than grade, stage, or any other clinicopathologic features. (medscape.com)
  • NEW YORK - Researchers at the University of California, Santa Cruz have pioneered a method for repeatedly sampling messenger RNA (mRNA) from single cells without killing the cells in question. (genomeweb.com)
  • The method, which was recently published in the journal PLoS One , relies on automated nanopipetting from slide-mounted, intact tissue and mRNA capture using a just-launched magnetic mRNA purification and sequencing library preparation platform from nanoparticle assay developer Nvigen. (genomeweb.com)
  • Where most scRNA-seq methods involve killing the cell from which messenger RNA is taken, the UCSC method leaves cells alive for further sampling, taking only between 1 and 5 percent of the cell's mRNA per sampling event. (genomeweb.com)
  • The utility of longitudinal data in single-cell spatial transcriptomics is well known and numerous methods attempt to address it. (genomeweb.com)
  • Gene expression quantification of the sections is implemented using spatial transcriptomics by reoriented projections and sequencing (STRP-seq), a method that combines the sampling strategy presented above with a customized, low-input RNA-seq protocol based on single-cell tagged reverse transcription sequencing (STRT-seq) chemistry. (scienceboard.net)
  • In this study, we aimed to develop a method for sample preparation for micro-proteomics on using the FFPE liver samples. (bvsalud.org)
  • High adoption compared to spatial genomic methods, for the transcriptomic study of single cells is a key segment driver. (grandviewresearch.com)
  • The various technologies differ in the collection process, possible imaging methods (fluorescence microscopy/bright field microscopy/differential interference contrast microscopy/phase contrast microscopy/ etc.) and the types of holders and tissue preparation needed before the imaging and isolation. (wikipedia.org)
  • Roger Deal and Steven Henikoff from the Fred Hutchinson Cancer Research Center have developed a cheap and easy method, dubbed "isolation of nuclei tagged in specific cell types" (INTACT), which is. (the-scientist.com)
  • Our method overcomes a common problem of heterogeneity of cancer tissues. (genengnews.com)
  • Research analysis on the global microdissection market identifies that the growing adoption of next-generation sequencing (NGS) will be one of the major factors that will have a positive impact on the growth of the market. (technavio.com)
  • The application of technologically advanced NGS help researchers to analyse multiple genomes at a time, whereas the conventional methods of sequencing such as Sanger sequencing helps in the analysis of one gene at a time. (technavio.com)
  • The rising demand for efficient forensic tools is motivating researchers to incorporate microdissection tools during analysis. (technavio.com)
  • What are the key outcomes of the five forces analysis of the global microdissection market? (technavio.com)
  • NGMLR and Sniffles are long-read sequencing analysis methods to study the longer fragments of DNA, a breakthrough that may yield critical information about how cancer genomes evolve. (agbt.org)
  • This allows researchers to gain insights into the spatial distribution of genes and their expression patterns, which is not possible with traditional genomics methods. (grandviewresearch.com)
  • Shuttle & Find is a unique way to combine imaging and analytical methods of light and electron microscopy . (antisel.gr)
  • The method produces parallel-slice projections for each gene by quantifying the reads that map to a transcript in each of the secondary sections. (scienceboard.net)
  • One of the aims of my research was to develop optimized qPCR (quantitative polymerase chain reaction)-based methods to enable valid comparison of the gene expression of promising gene candidates in individual neurons from human post mortem tissue from M. Parkinson patients with the gene expression of the same neurons from healthy control subjects. (leica-microsystems.com)
  • Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber. (justia.com)
  • By learning a map between the unpaired distributions of unperturbed and perturbed cells, CellOT outperforms current methods and generalizes the inference of treatment outcomes in unobserved cell types and patients. (nature.com)
  • In comparison to PCR-based methods of whole genome amplification, the Multiple Displacement Amplification (MDA) technology included in the QIAseq Single Cell DNA Library Kit UDI eliminates PCR duplicates and minimizes GC-bias, ensuring high library diversity and maximum genome coverage. (qiagen.com)
  • In more detail, we compared the differentially expressed genes identified after both amplification methods. (biomedcentral.com)
  • Using this method, samples from patients with diagnosed pancreatic cancer were compared to the healthy controls. (genengnews.com)
  • This technique requires preselection of target genes and sequencing methods with resolution limitations. (scienceboard.net)
  • In an earlier paper, we described a low-temperature microdissection technique and its application to the determination of sodium, potassium, and water in the nucleus and cytoplasm of the mature amphibian oocyte ( Century, Fenichel & Horowitz, 1970 ). (biologists.com)
  • In this study, we used the low-temperature microdissection technique to investigate the 22 Na exchange in the nucleus and cytoplasm of Rana pipiens oocytes incubated in 22 Na-Ringer's solution. (biologists.com)
  • For high-risk patients, the recommendations differ regarding the age at which to begin screening, as well as the frequency and method of screening. (medscape.com)
  • This method allowed us to zero in on a small population of cancer stem cells expressing CD24, a specific cell surface biomarker shown to correlate with the development and metastasis of pancreatic cancer," continues Dr. Lubman. (genengnews.com)
  • A limitation of this method is that IFP cannot reconstruct complex anatomical configurations. (scienceboard.net)
  • Similarly, single cell methods are vital in stem cell research and cancer biology, where isolated populations of primary cells are heterogeneous due to limitations in purification protocols, and it is often a minority cell population that is the most relevant. (justia.com)
  • The growing demand for high accuracy forensic tools is identified as one of the key trends that will stimulate growth in the microdissection market. (technavio.com)
  • His lab develops efficient computational methods for analyzing DNA sequencing data, including tools for genome assembly, genome alignment, genome clustering, microbial forensics, and metagenomics. (agbt.org)
  • Pourmand's lab had established the underlying method of sampling material from single cells while maintaining cell viability in 2014 and the more recent study represents a way to reliably do so at scale. (genomeweb.com)
  • This testing method sheds light on where the virus may replicate and how it moves across the placenta from the mother to the fetus and infects the fetuses' brains. (cdc.gov)
  • He is also interested in the large-scale infrastructure required for modern bioinformatics including storage and access methods for high throughput sequencing data. (agbt.org)
  • Existing methods for measuring transcript levels in single cells include RT-qPCR (1), single molecule counting using digital PCR (2) or hybridization probes (3, 4), and next generation sequencing (5). (justia.com)
  • Our method allows isolating high-quality RNA from nematode feeding sites that is suitable for downstream applications such as microarray experiments. (biomedcentral.com)
  • Few methods for three-dimensional structure modeling of nucleic acids from cryo-EM data exist. (nature.com)
  • CryoREAD, a fully automated DNA/RNA atomic structure modeling method based on deep learning, was developed to fill this gap. (nature.com)