• Also, gene set enrichment analysis (GSEA) and immune infiltrating analyses were conducted to understand function annotation and the role of the signature in the tumor microenvironment. (scielo.br)
  • Adipocytes represent a major cell type in the mammary tumor microenvironment and are important for tumor growth. (jci.org)
  • Adipocyte-derived factors involved in tumor progression include proteins such as adiponectin, leptin, TNF-α, monocyte chemotactic protein-1 (MCP-1), IL-6, and ECM components that control tumor cell behavior within the tumor microenvironment. (jci.org)
  • Dr. Pienta's research interests include the ecology of cancer, tumor microenvironment, metastasis, biomarker development and novel therapeutic development. (hopkinsmedicine.org)
  • Gemcitabine is a standard agent used in the treatment of pancreatic cancer, with known effects on the tumor immune microenvironment. (amegroups.org)
  • Tumor-infiltrating CD8+ T-cells and myeloid cells, harvested after the mice were treated for 2 weeks, were analyzed with flow cytometry to characterize the effect the chemo-immunotherapy regimen had on the tumor microenvironment (TME). (amegroups.org)
  • Recent studies suggested that strategies to reduce immunosuppressive cells, promote systemic T-cell responses and lymphocyte trafficking to the tumor microenvironment (TME) may improve efficacy. (bmj.com)
  • 1 This is due to multiple factors, including tumor heterogeneity, the immunosuppressive nature of the tumor microenvironment (TME) and innate or acquired resistance to treatment. (bmj.com)
  • Additionally, characterization of the tumor microenvironment such as interactions with immune cells remain largely unknown. (springer.com)
  • Apart from CSCs, there are a myriad of factors and interactions between various cell types and the tumor microenvironment (TME) that ultimately affect CCA progression. (springer.com)
  • Cancer research has been conducted using cultured cells as part of drug discovery testing, but conventional two-dimensional culture methods are unable to reflect the complex tumor microenvironment. (medsci.org)
  • However, the cellular environment under 2D culture is very different and often a poor reflection of the complex tumor microenvironment (TME) in vivo [ 1 ]. (medsci.org)
  • The tumor microenvironment in the context of colon cancer and with a focus on the inflammatory mediators leukotriene D 4 (LTD 4 ) and prostaglandins (PGE 2 ) and their respective receptors. (lu.se)
  • Our aim is to generate solid support for novel and complementary treatments of colon cancer patients that focus on the inflammatory component of the tumor microenvironment. (lu.se)
  • We investigate how the lack of these receptors affects tumour growth and the surrounding tumour microenvironment in different colon cancer models. (lu.se)
  • Her focus of research is the role of estrogen and thyroid hormones in colon cancer progression and their effects on inflammatory components of the tumor microenvironment, in a big female cohort. (lu.se)
  • Syrina is a pharmacist and her research in cell pathology focuses on neutrophils recruitment in colorectal cancers microenvironment and in cancer related inflammation. (lu.se)
  • Now, she is interested in the interaction between the immune cells (mainly mast cells) present in the colon cancer microenvironment and colon cancer cells. (lu.se)
  • Her focus is to target the tumor microenvironment for developing new and effective therapeutic strategies. (lu.se)
  • Furthermore, the scracht assay result suggested that ATRA was a potential reagent to prevent the invasion or metastasis of GIST cells. (biomedcentral.com)
  • Only the has-miR-10b involved in breast cancer invasion and metastasis and has-miR-512-3p appeared to be up-regulated (2-3-fold) in all three cell lines. (biomedcentral.com)
  • Early metastasis of the FEPro labeled 231BRL were demonstrated onT2*-weighted MRI and BLI within 1 week post IC injection of cells. (biomedcentral.com)
  • Diagnosis of TBL is challenging given its resemblance to basal cell carcinoma (BCC) but is important given possible malignant transformation and potential metastasis. (karger.com)
  • N-809 was well-tolerated and reduced 4T1 lung metastasis, decreased MC38 tumor burden and increased survival versus N-803+αPD-L1. (bmj.com)
  • Although the diverse tumor-promoting roles of NFκB in cancer cell proliferation, anti-apoptosis, angiogenesis, invasion and metastasis, are well established [ 3 - 9 ], much less is known about how p100, a precursor protein of NFκB2, acts as a tumor suppressor in many mammalian cells [ 10 ]. (oncotarget.com)
  • Cancer stem cells (CSCs) are thought to the underlying reason for cancer initiation, metastasis, and relapse. (springer.com)
  • Interleukin 15 (IL-15) has been evaluated as a potential treatment for solid tumors in clinical trials, but the effectiveness of systemic IL-15 administration as a monotherapy has not been realized. (frontiersin.org)
  • Our findings have immediate implications in the clinical treatment of advanced solid tumors. (bmj.com)
  • Cisplatin (cis-diamminedichloroplatinum II, CDDP) is a chemotherapeutic agent widely used in the treatment of several solid tumors, among them testicular cancer, lung cancer, breast cancer, and bladder cancer. (biomedcentral.com)
  • To characterize biomarkers in neural tumors, we analyzed the acidic lipid fractions of 13 neural tumor cell lines using enzyme-linked immunoabsorbent assay (ELISA) and high-performance thin-layer chromatography (HPTLC) immunostaining. (karger.com)
  • Our data indicate that certain GSL antigens, especially SGGLs, GD3, and OAc-GD3, are expressed in neural tumor cells and may be considered as tumor-associated antigens that represent important biomarkers for neural tumors. (karger.com)
  • Biomarkers for identification of these histologically identical WHO II tumors do not exist. (nature.com)
  • IL-15 is considered superior to IL-2 for cancer immunotherapy due to its strong bioactivity in improving CD8 + T and natural killer (NK) cell survival, proliferation and cytotoxic function ( 8 ). (frontiersin.org)
  • PANX1 channel inhibition using probenecid (PBN) or carbenoxolone (CBX) reduced the proliferation of our panel of high-risk NB cell lines. (jcancer.org)
  • We show that expression of the Y10F PANX1 mutant, which cannot be phosphorylated on tyrosine 10 and acts in a dominant-negative manner, curtailed NB cell proliferation. (jcancer.org)
  • Demethylating the hypermethylated REC8 gene restored its expression in thyroid cancer cells in which the PI3K pathway was genetically over-activated and induced expression of REC8 protein inhibited the proliferation and colony formation of these cells. (oncotarget.com)
  • IM combined with IR can synergistically inhibit the proliferation, apoptosis, clonogenesis, invasion, migration and cell cycle of GIST cells. (researchsquare.com)
  • Cell proliferation was determined by trypan blue dye exclusion test. (biomedcentral.com)
  • In this work, for the first time we have demonstrated that ATRA affected on cell proliferation of GIST-T1 and GIST-882 cell line through inhibition of cell growth in a dose dependent manner and induced apoptosis. (biomedcentral.com)
  • Thereafter, the downstream signaling pathways are activated promoting cell proliferation and/or survival. (biomedcentral.com)
  • AKAP12 knockdown in benign meningioma cells SF4433 increases proliferation, cell cycle, migration, invasion, and confers an anaplastic profile. (nature.com)
  • It plays a role in proliferation, apoptosis, angiogenesis, and tumor invasion ( 12 - 14 ), and its expression may be related to resistance to cytotoxic-, antiestrogen-, and HER2 / neu -targeted therapy ( 15 - 18 ). (snmjournals.org)
  • Testing this method on four cell lines, two human and two mouse, the team identified over 700 targets leading to cancer cell proliferation. (bigthink.com)
  • Cell cycle analysis suggested activation of apoptosis, without arresting cell cycle progression. (nature.com)
  • SVC112 increased cell-cycle progression delay and slowed DNA repair following radiation, enhancing colony and sphere formation radiation effects. (nih.gov)
  • Low AKAP12 expression in a larger cohort of patients (75) characterized tumor invasiveness, recurrence, and progression, indicating its potential as a prognostic biomarker. (nature.com)
  • Overexpression of XAF1 impairs tumor progression whereas knockdown of XAF1 promotes tumor growth, suggesting that XAF1 may be a candidate tumor suppressor in neuroblastoma and its associated pathway may be important for developing future interventions. (nus.edu.sg)
  • Knockdown of p100 in cultured human bladder cancer cells promoted anchorage-independent growth accompanied with elevating abundance of cell-cycle-related proteins and accelerated cell-cycle progression. (oncotarget.com)
  • This project is based on the hypothesis that LINE-1 elements constitute critical entities in glioma initiation and progression, by acting as a source of regulatory elements and coopted functional RNAs in tumor cells. (lu.se)
  • Efforts to link CD44 to CSCs and tumor progression should consider the expression of various CD44 isoforms. (lu.se)
  • These mediators and their receptors are enriched in colon cancer cells and colon cancer tissues, which demonstrate there, contribute to tumor development and progression. (lu.se)
  • Activation of Akt leads to the stimulation of antiapoptotic pathways, promoting cell survival. (aacrjournals.org)
  • Furthermore, GSEA and immune infiltrating analyses showed that the signature had close interactions with immune-related pathways and was closely related to CD8 T cells and monocytes in the tumor environment. (scielo.br)
  • Importantly, some of these differences in sensitivity were in concordance with high expression of the targets or activation of the corresponding pathways in primary tumor samples of the same subtype. (tudelft.nl)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Interestingly, both MO-MDSCs and PMN-MDSCs suppressed antigen-specific T-cell responses, albeit using distinct effector molecules and signaling pathways. (ashpublications.org)
  • She is investigating two different ligands and their receptive G-protein coupled receptors WNT5A/Frizzle and cysteinyl leukotriene C4/CysLTR2 signaling pathways which both upregulates 15-PGDH and induce re-differentiation of colon cancer cells. (lu.se)
  • More importantly, we show that MESF treatment leads to significant inhibition of tumor growth and a 3-fold increase in the life span of tumor bearing animals compared to untreated tumor mice. (nature.com)
  • Recent reports have shown that the sensitivity of non-small-cell lung cancer cell lines to EGFR inhibitors such as erlotinib (Tarceva, OSI Pharmaceuticals) is dependent on inhibition of the phosphatidylinositol 3′-kinase-phosphoinositide-dependent kinase 1-Akt-mTOR pathway. (aacrjournals.org)
  • Therefore, inhibiting EGFR alone may not be sufficient for substantial inhibition of all tumor cells, highlighting the need for multipoint intervention. (aacrjournals.org)
  • Therefore, in select cell lines, inhibition of both S6 and Akt was achieved only with the combination of erlotinib and rapamycin. (aacrjournals.org)
  • Intraperitoneal administration of IL-15cx results in a moderate inhibition of breast cancer growth that is associated with an increase in the frequency of cytotoxic CD8 T cells and the improvement of their function. (frontiersin.org)
  • Systemic and local administration of IL-15cx shows two distinct therapeutic responses, a moderate tumor growth inhibition or heterogeneous tumor regressions with survival improvement. (frontiersin.org)
  • One way to elicit an immunogenic response to immune checkpoint inhibition may be chemotherapy, as many preclinical and clinical studies have demonstrated improved anti-tumor responses and survival with combination chemotherapy/immunotherapy regimens ( 1 - 4 ). (amegroups.org)
  • Although the mechanism of the effect of the emitted qi still remains unknown, the results of the present research reveals the necessity as well as the possibility of further investigating the inhibition of tumors and its mechanism. (qigonginstitute.org)
  • Tumors are generated from human tumor cell lines or tissues, then dissected and digested using an enzyme cocktail of DNase I (Sigma 10104159001), trypsin inhibitor (Sigma T9253-5G), and papain (Worthington LS003126) into a single cell suspension. (stanford.edu)
  • Traditionally, CSCs are identified through a combination of surface markers, however, there is a lack of consensus among the scientific community, especially as these combinations appear to be specific to the corresponding tissues and tumors. (springer.com)
  • Tissues and Cells. (lu.se)
  • ETP augmented fibrosis, angiogenesis, and inflammation through recruitment of macrophages and endothelial cells. (jci.org)
  • Methods: We tested this hypothesis by exposing SPD-MAA to macrophages and measuring functions. (cdc.gov)
  • 0.05), and trended towards decreasing tumor-associated macrophages (TAMs) (P=0.18). (amegroups.org)
  • Moreover, in contrast to N-803+αPD-L1, N-809 reduced immunosuppressive regulatory T cells (T reg ), monocytic myeloid-derived suppressor cells (M-MDSC) and M2-like macrophages in the TME. (bmj.com)
  • In addition, N-809 reduces immunosuppressive and pro-tumorigenic immune cells in the TME, including T reg , M2-like macrophages and M-MDSC. (bmj.com)
  • Following verification of a stem-like signature (upregulated expression of stemness markers, resistance to chemotherapy, increased spheroid formation, and tumorigenesis capabilities despite inoculation of a small number of cells), we analyzed the interaction of these cells with macrophages via direct and indirect coculture assays. (springer.com)
  • These studies suggest that there is a bi-directional crosstalk between macrophages and CSCs that promotes stemness renewal and tumor associated macrophage polarization. (springer.com)
  • Here we show that methanolic extracts of Sapota fruit (MESF) induces cytotoxicity in a dose-dependent manner in cancer cell lines. (nature.com)
  • Annexin V-propidium iodide double-staining demonstrated that Sapota fruit extracts potentiate apoptosis rather than necrosis in cancer cells. (nature.com)
  • In a preliminary study it has been shown that phenolic antioxidants such as methyl 4- O -galloylchlorogenate and 4- O -galloylchlorogenic acid derived from Sapota fruits can induce cytotoxicity in colon cancer cells 23 . (nature.com)
  • The goal of this study was to examine the activity of IL-15/IL-15Rα complex (IL-15cx) to CD8 + T cells and evaluate its potential efficacy in murine breast cancer models. (frontiersin.org)
  • The depletion of myeloid-derived suppressor cells (MDSCs) has no impact on mouse breast cancer growth. (frontiersin.org)
  • One of these poorly immunogenic tumors is breast cancer with response rates of only 0%-18.5% to CPI therapy alone ( 2 - 4 ). (frontiersin.org)
  • PD-L1 is expressed mainly on tumor-infiltrating immune cells in roughly 41% of patients with triple negative breast cancer ( 5 ). (frontiersin.org)
  • Desmoplastic small-round-cell tumor (DSRCT) is an aggressive and rare cancer that primarily occurs as masses in the abdomen. (wikipedia.org)
  • The disease rarely occurs in females, but when it does the tumors can be mistaken for ovarian cancer. (wikipedia.org)
  • The tumor appears to arise from the primitive cells of childhood, and is considered a childhood cancer. (wikipedia.org)
  • In males DSRCT may be mistaken for germ cell or testicular cancer while in females DSRCT can be mistaken for ovarian cancer. (wikipedia.org)
  • Cancer stem cells (CSC) drive growth, therapy resistance, and recurrence in head and neck squamous cell carcinoma (HNSCC). (nih.gov)
  • SVC112 preferentially inhibited cancer cells compared with patient-matched cancer-associated fibroblasts, whereas HHT was equally toxic to both. (nih.gov)
  • SIGNIFICANCE: Inhibiting protein elongation with SVC112 reduces tumor growth in head and neck squamous cell carcinoma and increases the effects of radiation by targeting the cancer stem cell pool. (nih.gov)
  • Brain tumors are the most common solid tumor in pediatrics, accounting for 23.7% of new cancer diagnoses in children ( 1 1. (scielo.br)
  • Dublin, May 26, 2023 (GLOBE NEWSWIRE) -- The "Squamous-Cell Carcinoma of the Larynx in the United States, 2022-2042: Cancer Populations USA Report and Data Dashboard" report has been added to ResearchAndMarkets.com's offering. (globenewswire.com)
  • Neuroblastoma (NB) is the most common extracranial solid tumor in childhood, accounting for 7-10% of pediatric cancers and 15% of all pediatric cancer deaths [ 1 , 2 ]. (jcancer.org)
  • To investigate the effect of gambogic acid (GA) on apoptosis in the HT-29 human colon cancer cell line. (wjgnet.com)
  • Collagen VI (COL6) is highly expressed in adipose tissue, upregulated in the obese state, and enriched in breast cancer lesions and is a stimulator of mammary tumor growth. (jci.org)
  • Even though the idea of an immune system actively searching for and disposing of potential mutated tumor cells is over a century old, only recent developments in various fields such as mass spectrometry, immuno-checkpoint blockade strategies and in silico modelling have enabled the realization of the full potential of recruiting immune system to fight cancer and the possibilities of personalized therapies. (helsinki.fi)
  • These therapeutic methods, including but not limited to oncolytic virus therapies, T-cell therapies and cancer vaccines, are based on the body's ability to recognize mutated antigen peptides presented on the cell surface by MCH-receptors (also known as HLA-receptors in humans) and the disposal of the malignant cells by cytotoxic T-cells. (helsinki.fi)
  • This master thesis is a part of a project aiming to set up immunoaffinity-purification/MS based method in order to analyse the ligandome and determine T-cell recognized cancer associated antigens from tumor cells. (helsinki.fi)
  • Preliminary MTS assay is used to determine linear range and optimal time frame for the PBMC/cancer cell co-culture killing assay. (helsinki.fi)
  • Another challenge is the cell entry of oncolytic adenovirus, which is mainly mediated by the Coxsackie-Adenovirus receptor and this receptor is downregulated in various cancer cells. (helsinki.fi)
  • In order to overcome these limitations, this study explored the possibility to use cancer cell derived extracellular vesicles (EVs) as drug delivery system for oncolytic adenovirus. (helsinki.fi)
  • Through a genome-wide search for such genes that were epigenetically controlled by the PI3K pathway in thyroid cancer cells, we found a wide range of genes with broad functions epigenetically targeted by the PI3K pathway. (oncotarget.com)
  • Follicular cell-derived thyroid cancer is a common endocrine malignancy, which histologically consists of papillary thyroid cancer (PTC), follicular thyroid cancer (FTC) and anaplastic thyroid cancer (ATC) [ 1 - 3 ]. (oncotarget.com)
  • Current treatment methods for breast cancer include invasive surgical procedures, radiotherapy, hormone therapy and chemotherapy. (amrita.edu)
  • Cervical cancer cell lines (HeLa, SiHa, CaSki and CaLo) were obtained from ATCC and cultured as monolayers in Dulbecco Modified Eagle's Medium (DMEM) containing 10% (V/V) fetal bovine serum (GIBCO, Bethesda, MD, USA) at 37°C in a humidified atmosphere of 5% (V/V) CO 2 . (biomedcentral.com)
  • Our objective was to determine if CDK2AP1 mRNA expression levels were consistent with tumour-suppressive functions in breast cancer. (cancerindex.org)
  • Extracts from 18 of the plants under investigation were found to substantially suppress the growth of a cancer cell line in the culture dish. (sciencedaily.com)
  • In contrast, a whole range of standard anti-cancer drugs fail to be effective against this cell. (sciencedaily.com)
  • Currently, we are aligning the effectiveness of the substances on 60 different cancer cell lines with the gene activity profiles of these cells. (sciencedaily.com)
  • Thereby, it may be possible to discover whole new Achilles' heels of the cancer cell," said Efferth describing the next steps. (sciencedaily.com)
  • Using the brain seeking MDA-MB-231BR transfected with luciferase gene, a metastatic breast cancer brain tumor model was investigated in the nude rat. (biomedcentral.com)
  • Brain seeking breast cancer cells MDA-MB-231BR transfected with firefly luciferase (231BRL) were labeled with ferumoxides-protamine sulfate (FEPro) and 1-3 × 10 6 cells were intracardiac (IC) injected. (biomedcentral.com)
  • MRI and BLI were performed up to 4 weeks to monitor the early breast cancer cell infiltration into the brain and formation of metastases. (biomedcentral.com)
  • Fluorescent microscopy demonstrated the presence of 231BR-EGFP cancer cells located within the cerebral vasculature in approximately the same location as the hypointense voxels on MRI. (biomedcentral.com)
  • In the present study, the venoms from five sea anemones (Heteractis crispa, Heteractis magnifica, Heteractis malu, Cryptodendrum adhaesivum and Entacmaea quadricolor) were obtained by the milking technique, and the potential of these venoms to kill cancer cells was tested on three cell lines (A549 lung cancer, T47D breast cancer and A431 skin cancer). (scielo.br)
  • The results indicate that the sea anemone venom is cytotoxic to human cancer cells. (scielo.br)
  • sea anemone venoms, cytolysin, cancer cell line. (scielo.br)
  • Taken together, our studies not only for the first time establish p100 as a key tumor suppressor of bladder cancer growth, but also identify a novel molecular cascade of PHLPP2/CREB/miR-302d that mediates the tumor suppressive function of p100. (oncotarget.com)
  • In the process of tumor invasion, cancer cells leave the primary tumor either individually or collectively ( Friedl and Wolf, 2003 ). (elifesciences.org)
  • there is a need to quantify the forces that are collectively exerted by invading cancer cells under physiologically relevant conditions. (elifesciences.org)
  • Concerns that this virus might infect humans through exposure to food products from subclinically infected animals prompted 10 studies that used what were then (1975-1979) state-of-the-art immunologic methods to test serum samples from a collective total of 1,761 humans, including cancer patients, farm workers, and veterinarians ( 5 ). (cdc.gov)
  • However, due to their elusive character and differences in identification among different types of cancer, it remains a challenge to study such cells. (springer.com)
  • Here, we employ a fluorescent reporter system to track and isolate stem-like cancer cells of cholangiocarcinoma cell lines. (springer.com)
  • Cancer stem cells (CSCs) represent a small subset of the tumor population and reside at the apex of the hierarchy [ 9 ]. (springer.com)
  • The purpose of this study was to establish a 3D culture method for oral cancer and to verify its practicality. (medsci.org)
  • Three-dimensional cultures were performed using several oral cancer cell lines. (medsci.org)
  • Spheroids made from oral cancer cell lines appeared to have tumor-like characteristics that may reflect their clinical significance. (medsci.org)
  • In the future, it may become possible to produce tumor spheroids from tissue samples of oral cancer patients, and then apply them to drug screening and to develop individualized diagnostic and treatment methods. (medsci.org)
  • A commonly used in vitro experimental method in cancer research is two-dimensional (2D)-culture using dishes or flasks. (medsci.org)
  • Furthermore, it is presumed that most of the characteristics inherent in cancer cells are lost. (medsci.org)
  • In vitro, the affinity and internalization kinetics of 111 In-R1507 were determined using the IGF-1R-expressing triple-negative breast cancer cell line SUM149. (snmjournals.org)
  • Although they account for only 10%-17% of all breast carcinomas ( 1 - 8 ), tumors are often high grade and patients are relatively young and have a reduced breast cancer-specific survival, compared with patients with estrogen receptor- or HER2 / neu -expressing tumors ( 2 , 9 ). (snmjournals.org)
  • Researchers at Northwestern have discovered a genetic "kill code" that might enable the destruction of cancer cells. (bigthink.com)
  • This novel new therapy "downstream" of chemo might destroy cancer cells without affecting the body's immune system. (bigthink.com)
  • Along with the suppression and cessation of growth of cancer cells, numerous important cells are also damaged, stressed, or destroyed. (bigthink.com)
  • Published in the journal Nature Communications , researchers at Northwestern University have discovered a genetic "kill code" that could enable the destruction of cancer cells without the "spray and pray" mentality of chemotherapy. (bigthink.com)
  • The hardest part of cancer research thus far has been finding a way to destroy cancer cells without affecting the rest of the body's networks. (bigthink.com)
  • The small RNA molecules can effectively kill cancer cells, a process that chemotherapy is meant to activate. (bigthink.com)
  • They found that chemotherapy drugs kill cancer cells in part by triggering the release of a toxic mechanism, resulting in the release of tumor-suppressive miRNAs. (bigthink.com)
  • In turn, as a result of research into the abnormal cancer cell, the basic understanding of the cell has greatly improved. (medscape.com)
  • Cancer is characterized by uncontrolled growth and division of a cell, with extension beyond the normally limiting basement membrane and through the boundaries of normal cells. (medscape.com)
  • This is the multihit theory of tumorigenesis, in which a series of multiple triggering events in the genetic and cellular makeup of a cell ultimately cause cancer. (medscape.com)
  • [ 6 ] These events lead the cancer cell to escape normal cell growth and control mechanisms, to avoid system control mechanisms (ie, immunologic surveillance), and to establish a nutrient supply. (medscape.com)
  • p21 and other tumor suppressor genes' inactivation are therefore most likely involved in the first steps of the cancer model, with later stages possibly involving protooncogene activation and inflammation. (medscape.com)
  • CD44 isoforms are heterogeneously expressed in breast cancer and correlate with tumor subtypes and cancer stem cell markers. (lu.se)
  • The CD44 cell adhesion molecule is aberrantly expressed in many breast tumors and has been implicated in the metastatic process as well as in the putative cancer stem cell (CSC) compartment. (lu.se)
  • Breast cancer cell lines showed a heterogeneous expression pattern of the CD44 isoforms, which shifted considerably when cells were grown as mammospheres. (lu.se)
  • arations verified the presence of cancer cells in all samples. (lu.se)
  • The Nilsson lab focuses on how inborn genetic variation influences blood cell formation and blood cancer risk in humans. (lu.se)
  • Lubnas research is mainly focused on the tumor suppressor protein 15-hydroxyprostaglandin dehydrogenase which is known to be down-regulated in colon cancer. (lu.se)
  • Her research involves methods as tissue microarray (TMA) of colon cancer patients for immunohistochemistry (IHC) and Western blot, qPCR, transfection, luciferase assay, immunofluorescens on colon cancer cell lines. (lu.se)
  • We performed a first-of-kind evaluation of two transcriptome-based precision cancer medicine methodologies to predict tumor sensitivity to a comprehensive repertoire of clinically relevant oncology drugs, whose mechanism of action we experimentally assessed in cognate cell lines. (cdc.gov)
  • A goal of the project is for a candidate to unravel new ways to think about and approach early cancer detection, with methods, tools, or thinking from any field within the medical or natural sciences. (lu.se)
  • Machine learning models for identifying predictors of clinical outcomes with first-line immune checkpoint inhibitor therapy in advanced non-small cell lung cancer. (cdc.gov)
  • Analytical and clinical validation of a custom 15-gene next-generation sequencing panel for the evaluation of circulating tumor DNA mutations in patients with advanced non-small-cell lung cancer. (cdc.gov)
  • Herein, we sought to determine if rapamycin, an inhibitor of mTOR, could enhance erlotinib sensitivity for cell lines derived from a variety of tissue types (non-small-cell lung, pancreatic, colon, and breast). (aacrjournals.org)
  • The tumor is classified as a soft tissue sarcoma and a small round blue cell tumor. (wikipedia.org)
  • These effects were partially mediated through enhanced TGF-β signaling, which contributes to tissue fibrosis and epithelial-mesenchymal transition (EMT) of tumor cells. (jci.org)
  • We performed semi quantitative RT-PCR on 41 cervical tumor and 6 normal tissue samples. (biomedcentral.com)
  • With the development of tumor molecular heterogeneity theory(12), there are still some patients with GIST who are sensitive to radiotherapy, especially for the patients with advanced stage(13-16).In addition, with the development of imaging technology and modern tissue and organ radiation technology, it has become a reality to concentrate high-dose radiation locally in abdominal cavity tumors(12), which challenges RT's insensitivity to GISTs. (researchsquare.com)
  • Tumor tissue or cells can be implanted into the specific orthotopic sites of the mice. (stanford.edu)
  • We show that our method is accurate up to strains of 50% and remains valid even for irregularly shaped tissue samples when considering only the deformations in the far field. (elifesciences.org)
  • In this work, we introduce a computationally and experimentally simple and reliable method that captures collective effects in tissue remodeling and thus facilitates screenings of potential force-targeting agents. (elifesciences.org)
  • H-E staining showed tumor tissue-like structures specific to each cell line. (medsci.org)
  • Therefore, it is difficult to propose that 2D culture accurately reflects the original growth state of tumor tissue. (medsci.org)
  • Thus far, studies on IGF-1R expression have been performed mainly on tumor tissue sections using immunohistochemistry. (snmjournals.org)
  • ANA is the screening method of choice for systemic rheumatic diseases (as sometimes referred to as connective tissue or collagen diseases) such as systemic lupus erthyematosus (SLE), mixed connective tissue disease, Sjögren syndrome, scleroderma, CREST syndrome, rheumatoid arthritis, polymyositis, and dermatomyositis. (medscape.com)
  • The methods can include a variety of mass spectrometry tools, cell culture, tissue sectioning, mathematical mass balance modelling, and isotope geochemistry. (lu.se)
  • Cell survival was determined 7, 8, or 9 days after RT by the sulforhodamine B test. (biomedcentral.com)
  • Despite current treatment regimens that include multimodal cytotoxic therapies and aggressive surgery, the survival rate of high-risk NB patients remains below 50%, while patients with refractory or relapsed tumors have no cure [ 6 ]. (jcancer.org)
  • The cytotoxicity on different cell lines was assayed using the 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) assay which measures survival based on the detection of mitochondrial activity and by the crystal violet assay, which measures survival based on the ability of cells to remain adherent to microplates. (scielo.br)
  • Overall, these novel effects of N-809 promote an inflamed TME, leading to lower tumor burden and increased survival. (bmj.com)
  • Previous studies have juxtaposed and analyzed immune cell compartments between different CCA categorizations such as short or long-term overall survival (OS) [ 16 ]. (springer.com)
  • Unsupervised hierarchical cluster analysis of CD44 isoform expression data divided tumors into four main clusters, which showed significant correlations to molecular subtypes and differences in 10-year overall survival. (lu.se)
  • Cell apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling and Hoechst 33342 staining, and quantified by flow cytometry. (wjgnet.com)
  • Smac resides in the mitochondrial intermembrane space in healthy cells but is released into the cytosol during apoptosis, where it interacts with IAPs and disrupts their ability to bind caspases [ 8 ]. (biomedcentral.com)
  • In further examination, we found that the ATRA-induced apoptosis in GIST-T1 cells was accompanied by the down-regulated expression of survivin and up-regulated expression of Bax protein. (biomedcentral.com)
  • The dCK activity of our cell lines varied considerably and differed up to three fold from 5 to 15 pmol/h/mg protein between the tested cell lines. (biomedcentral.com)
  • The antitumor efficacy was studied in mouse mammary carcinoma models (Her2/neu transgenic and 4T1-luc mammary cancers) treated with systemic recombinant protein with/without the depletion of myeloid-derived suppressor cells or intra-tumoral gene electrotransfer (GET). (frontiersin.org)
  • This study characterizes the expression of hormone receptors, and the mRNA and DNA methylation levels of docking protein 7 (DOK7), and E74-like factor 5 (ELF5), in 21 novel tamoxifen-resistant cell lines and extends the findings to primary and recurrent human breast tumors. (umass.edu)
  • The parent (MCF-7) and tamoxifen-selected cell lines were characterized for protein expression of ER, progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2) using immunohistochemistry (IHC). (umass.edu)
  • High dose of ATRA induced morphologic change in GIST-T1 cells, rounded-up cells, and activated the caspase-3 protein. (biomedcentral.com)
  • Moreover, ATRA suppressed the activity of KIT protein in GIST-T1 cells and its downstream signal, AKT activity, but not MAPK activity. (biomedcentral.com)
  • This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. (cancerindex.org)
  • This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. (cancerindex.org)
  • The substances were found to suppress the growth of a specific tumor cell line that is particularly resistant to many commonly used cytotoxins due to overproduction of a transport protein in the cell wall. (sciencedaily.com)
  • Although the precursor protein of NFκB2 (p100) is thought to act as a tumor suppressor in mammalian cells, the molecular mechanism of its anti-tumor activity is far from clear. (oncotarget.com)
  • The researchers hope this is a step toward a straightforward and cost-effective method of generating novel benzoxazole analogs through protein engineering and combinatorial biosynthesis. (tamu.edu)
  • Western blotting was used for protein expression analysis of the collected cell masses (spheroids), and H-E staining was used for structural observation. (medsci.org)
  • MUC1 mRNA and protein expression, and KL-6 levels in cell line supernatants were investigated in PM primary cell lines in vitro. (lu.se)
  • ER protein determinations were performed using two-thirds of all breast cancers are ER at the time of diagnosis, the standard methods in the routine clinical laboratory (9). (lu.se)
  • CpG-conditioned CD8 + T cells acquired a unique proteomic signature hallmarked by an IL-2Rα high ICOS high CD39 low phenotype and an altered metabolic profile, all reliant on B cells transiently present in the culture. (bmj.com)
  • CpG fostered the expansion of potent CD8 + T cells with the signature phenotype and antitumor ability via empowering a direct B-T cell interaction. (bmj.com)
  • Isolated B cells also imparted T cells with the CpG-associated phenotype and improved tumor immunity without the aid of additional antigen-presenting cells or other immune cells in the culture. (bmj.com)
  • Moreover, we describe for the first time an association of the up-regulation of micro-RNA species such as hsa-miR-512-3p/-515/-517/-518/-525 and down-regulation of hsa-miR-99a/-100/-145 with a cisplatin resistant phenotype in human germ cell tumors. (biomedcentral.com)
  • Introduction: The contribution of radial migration lines (RMLs) to the neuroanatomical and neurocognitive phenotype of tuberous sclerosis complex (TSC) is unclear. (eur.nl)
  • Tumors characterized as positive for the CD44+/CD24- phenotype by immunohistochemistry were associated to all isoforms except the CD44 standard (CD44S) isoform, which lacks all variant exons. (lu.se)
  • In contrast, the previously established ER-negative TMX2-28 cell line has decreased expression of both DOK7 and ELF5 and increased DNA methylation in the transcriptional start site region of these genes. (umass.edu)
  • Cells will be labeled with luciferase or other reporter genes. (stanford.edu)
  • Total RNA from tumors the expression of thousands of genes (3) to address complex questions was isolated using two successive rounds of Trizol. (lu.se)
  • In a second line, we use natural genetic variation to find genes that regulate hematopoietic stem and progenitor cells (HSPC) in humans. (lu.se)
  • Methods We applied a new unsupervised, iterative approach to stratify CRC tumor samples into subtypes based on genome-wide mRNA expression data. (tudelft.nl)
  • Congruently, high tumor cell MUC1 mRNA expression in primary cell lines associated with prolonged corresponding patient OS (HR 0.35, p = 0.004). (lu.se)
  • Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal neoplasms occurring throughout the entire region of the gastrointestinal tract and are considered to originate from intestitial cells of Cajal, the pacemaker cells of the gut [ 1 ]. (biomedcentral.com)
  • ELF5 methylation was lower in second versus primary tumors in women who received anti-estrogen treatment, in PR-negative versus PR-positive tumors, and in the subset of PR-positive first tumors from the group of women who had second PR-negative tumors as compared to those who had second PR-positive tumors. (umass.edu)
  • CONCLUSIONS: The distinct ELF5 methylation of PR-positive primary tumors from women who had a PR-negative recurrence indicates the possibility of stratification of women for tailored treatment in the early stages of disease. (umass.edu)
  • Genomic studies have provided insights into molecular subgroups and oncogenic drivers of pediatric brain tumors (PBT) that may lead to novel therapeutic strategies. (scielo.br)
  • Cacciotti C, Fleming A, Ramaswamy V. Advances in the molecular classification of pediatric brain tumors: a guide to the galaxy. (scielo.br)
  • Gamma deficits as a neural signature of cognitive impairment in children treated for brain tumors. (scielo.br)
  • 7. Mabbott DJ, Snyder JJ, Penkman L, Witol A. The effects of treatment for posterior fossa brain tumors on selective attention. (scielo.br)
  • Working together with colleagues in Mainz and Düsseldorf, Germany, Graz, Austria and Kunming in China, he launched a systematic compound search in 76 Chinese medicinal plants that are believed to be effective against malignant tumors and other growths. (sciencedaily.com)
  • No significant difference was found between malignant tumors from oral or cutaneous origin. (avma.org)
  • The latter contains three ingredients with powerful anti-tumor activity. (sciencedaily.com)
  • Our results highlight the crucial role of ETP as an obesity-associated factor that promotes tumor growth in the context of adipocyte interactions with tumor and stromal cells. (jci.org)
  • Besides, administration of concentrated extracts of Morinda citrifolia in tumor containing animals increased the immune response of the animals with a concomitant reduction in tumor burden 7 . (nature.com)
  • Additional information of PD-L1 expression can be used to evaluate cell line's immune-evasion abilities. (helsinki.fi)
  • Thus, N-809 can potentially stimulate effector immune cells through IL-15 and block immunosuppressive PD-L1. (bmj.com)
  • Methods The ability of N-809 to block PD-L1 and induce IL-15-dependent immune effects was examined in vitro and in vivo. (bmj.com)
  • Antitumor efficacy of N-809 or N-803+αPD-L1 was evaluated in two murine carcinoma models and an extensive analysis of immune correlates was performed in the tumor and tumor-draining lymph node (dLN). (bmj.com)
  • The induction of CD11b + Gr-1 + myeloid-derived suppressor cells (MDSCs) is an important immune-evading mechanism used by tumors. (ashpublications.org)
  • Identifying the eight-gene signature ( CBX7 , JADE2 , IGF2BP3 , OR2W6P , PRAME , TICRR , KIF4A , and PIMREG ) could accurately identify patients' prognosis and the signature had close interactions with the immunodominant tumor environment, which may provide insight into personalized prognosis prediction and new therapies for PBT patients. (scielo.br)
  • These findings are consistent with REC8 being a novel major bona fide tumor suppressor gene and a robust epigenetic target of the PI3K pathway. (oncotarget.com)
  • The effects on dopamine (DA) release and dopamine transporter (DAT) gene expression were assayed in hypothalamic HypoE22 cells. (researchgate.net)
  • In the same experimental system, IL-10 and BDNF gene expressions increased, and in HypoE22 cells, the extract decreased the extracellular dopamine level and increased the DAT gene expression due to the direct interaction of parthenolide with the DAT. (researchgate.net)
  • This gene thus plays a role in both cell-cycle and epigenetic regulation. (cancerindex.org)
  • Compared with N-803+αPD-L1, N-809 enhanced natural killer (NK) and CD8 + T-cell activation and function in the dLN and TME, relating to increased gene expression associated with interferon and cytokine signaling, lymphoid compartment, costimulation and cytotoxicity. (bmj.com)
  • While RelA/p50 heterodimer is the predominantly form and plays a major regulatory role in mammalian cells, the RelB/p100 is also expressed and acts as the alterative pathway regulating its downstream gene expression [ 2 ]. (oncotarget.com)
  • However, unlike other oncogenic retroviruses, deltaretroviruses have an additional region, tax (trans-activating region of the X gene), which has regulatory functions and is oncogenic to host cells. (cdc.gov)
  • In a first line of research, we study genetic predisposition for multiple myeloma (MM). Studies going back to the 1920s support familial aggregation of MM. Recently, we and others discovered sequence variants influencing MM risk, including common variants at 24 loci and rare, high-penetrant variants in the tumor suppressor gene DIS3. (lu.se)
  • Modelled estimates are based on the best practice industry methods to estimate recurrence events and, where required, make imputations and extrapolations. (globenewswire.com)
  • We are combining natural substance research with advanced analytical and molecular-biological methods", Efferth explains. (sciencedaily.com)
  • ParetoTI theory identified four tumour archetypes within the tetrahedron, corresponding to the three previously reported molecular groups, and the fourth enriched for the aggressive supra-carcinoids. (who.int)
  • Clinical decisions by the molecular tumor board on comprehensive genomic profiling tests in Japan: A retrospective observational study. (cdc.gov)
  • Imatinib, a selective tyrosine kinase inhibitor, has been used as a standard first-line therapy for irresectable and metastasized gastrointestinal stromal tumor (GIST) patients. (biomedcentral.com)
  • The present study investigated whether KL-6 can serve as a diagnostic and/or prognostic biomarker in PM. Materials and methods: Using a fully-automated chemiluminescence enzyme immunoassay (CLEIA) for KL-6 and SMRP, pleural effusion samples from 87 consecutive patients with PM and 25 patients. (lu.se)
  • IL-15cx shows superior in vivo bioactivity to expand CD8 T cells in comparison to an equimolar single chain IL-15. (frontiersin.org)
  • T-bet is partially involved in CD8 T cell expansion ex vivo and in vivo due to IL-15 or IL-15cx. (frontiersin.org)
  • We posited that TLR agonists could be repurposed ex vivo to condition T cells with remarkable potency in vivo, circumventing TLR-related toxicity. (bmj.com)
  • Methods In this study we investigated how tumor-specific murine CD8 + T cells and human tumor infiltrating lymphocytes (TILs) are impacted when expanded ex vivo with the TLR9 agonist CpG. (bmj.com)
  • T cells expanded ex vivo from a CpG-treated culture demonstrated potent antitumor efficacy and prolonged persistence in vivo. (bmj.com)
  • Tumor cells can be cultured by our staff in preparation for in vivo studies. (stanford.edu)
  • Therefore, the aim of the present study was to develop a noninvasive, in vivo imaging method, using radiolabeled antibodies, to visualize IGF-1R expression. (snmjournals.org)
  • In vivo, the pharmacodynamics of 111 In-R1507 and 125 I-R1507 were determined in mice with subcutaneous SUM149 tumors. (snmjournals.org)
  • We will then use CRISPR approaches to silence/activateLINE-1 elements in glioma cell lines and interrogate the consequences of LINE-1 modulation on tumor physiology. (lu.se)
  • Excisional biopsy revealed a multinodular basaloid tumor, located within the dermis and subcutaneous fat that extended into skeletal muscle at the deep and peripheral margin. (karger.com)
  • 111 In-R1507 SPECT and 89 Zr-R1507 PET images of mice with subcutaneous SUM149 tumors were acquired at 1, 3, and 7 d after injection. (snmjournals.org)
  • The mechanism behind malignancy and tumor genesis is unknown, limiting development of alternative therapies. (nature.com)
  • While there are many applications and complications associated with chemotherapy, the commonly understood mechanism is the paralyzing of bone marrow, which leads to lower amounts of red and white blood cells and platelets. (bigthink.com)
  • 3. Feasibility of in silico methods in the prediction of immunogenic peptides was explored. (helsinki.fi)
  • In silico methods were successfully used to characterize previously identified HLA-restricted peptides and one previously identified immunogenic T-cell epitope. (helsinki.fi)
  • Immunotherapy can take advantage of the immunogenic response that chemotherapy elicits in tumors. (amegroups.org)
  • In this report on squamous-cell carcinoma of the larynx (SCC-L), forecast estimates are provided of incident cases by stage, age and gender, race-ethnicity, anatomical subsite, and PD-1/PD-L1 status. (globenewswire.com)
  • In the first experiment, a qigong master treated the poorly differentiated nasopharyngeal squamous cell carcinoma cell line (CNE-2) with his emitted qi, followed by the innoculation of the treated cell (qigong group) and the untreated cells (control group) into 16 female nude mice (NC-Z strain mice). (qigonginstitute.org)
  • A pathological examination further confirmed that there were no tumo cells in the inoculating site of the qigong group, whereas poorly differentiated squamous cell carcinomas were observed in tumor of the control group. (qigonginstitute.org)
  • An image depicting head and neck squamous cell carcinoma in vitro can be seen below. (medscape.com)
  • Head and neck squamous cell carcinoma in vitro (cell culture). (medscape.com)
  • BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
  • Microarrays were prepared in tumor biology. (lu.se)
  • The team works at the intersection between tumor biology, evolution, chemistry, and geology. (lu.se)
  • Objective -To analyze the expression of versican and hyaluronan in melanocytomas and malignant melanomas of dogs, to correlate their expression with expression of the hyaluronan receptor CD44, and to identify enzymes responsible for the synthesis and degradation of hyaluronan in canine dermal fibroblasts and canine melanoma cell lines. (avma.org)
  • Sample Population -35 biopsy specimens from melanocytic tumors of dogs, canine primary dermal fibroblasts, and 3 canine melanoma cell lines. (avma.org)
  • Canine fibroblasts and melanoma cell lines expressed hyaluronan synthase 2 and 3 (but not 1) and hyaluronidase 1 and 2. (avma.org)
  • There were no significant associations between CDK2AP1 expression and estrogen receptor status, tumour grade and tumour type. (cancerindex.org)
  • Triple-negative breast tumors are estrogen receptor-, progesterone receptor-, and HER2 / neu -negative. (snmjournals.org)
  • In contrast, fucosyl-GM1 was not found to restrict to small cell lung carcinoma cells. (karger.com)
  • But in the control group of the first experiment, tumors metastasized to the lung in mouse 2. (qigonginstitute.org)
  • Appropriate number of cells will be prepared into single cell suspension in 50-100μL volume (PBS:matrigel=1:1) and injected subcutaneously on the flank or the abdominal area of the mice. (stanford.edu)
  • Cells are mixed with matrigel (Corning 356237, non-phenol red, low growth factor) and injected into mice for passaging at 2-5million cells per 50μl SQ. Additional cells can be frozen down for future usage of P1 and P2 passages. (stanford.edu)
  • Tumor fragments from tumor growing in donor mice will be passaged to recepient mice. (stanford.edu)
  • Following the double-blind method, we carried out two experiments by using cell cultures and nude mice. (qigonginstitute.org)
  • No tumors were found in the livers and lungs of the mice in the qigong group. (qigonginstitute.org)
  • No tumors were found in the liver of mice in the first experiment or in the livers and lungs of the other mice. (qigonginstitute.org)
  • The degree of radiosensitisation was also cell line dependent and seemed to correlate with the sensitivity of the cell line to the cytotoxic effect of dFdC. (biomedcentral.com)
  • An early increase of local cytotoxic cells correlates with GET treatment and an increase of long-term memory T cells results from animals with complete tumor regression. (frontiersin.org)
  • It also delivers its function without activating regulatory T cells (Tregs) ( 9 ) and mediating activation-induced cell death of effector cytotoxic T cells ( 10 ). (frontiersin.org)
  • This combination regimen also increased the infiltration of CD8+ T-cells that expressed both PD1 and CD137, and these T-cells were shown to express high levels of interferon-gamma, a marker of cytotoxic effector CD8+ T-cells. (amegroups.org)
  • For example, some natural benzoxazoles show promising cytotoxic activity that fights against various tumor cell lines. (tamu.edu)
  • Conclusions Our results demonstrate a novel way to use TLR agonists to improve immunotherapy and reveal a vital role for B cells in the generation of potent CD8 + T cell-based therapies. (bmj.com)
  • Conclusions and Clinical Relevance -Versican may be useful as a diagnostic marker for melanocytic tumors in dogs. (avma.org)
  • Akt also regulates the mammalian target of rapamycin (mTOR)-S6K-S6 pathway to control cell growth in response to growth factors and nutrients. (aacrjournals.org)
  • SVC112 alone inhibited the growth of patient-derived xenografts (PDX), and SVC112 combined with radiation resulted in tumor regression in HPV-positive and HPV-negative HNSCC PDXs. (nih.gov)
  • Moreover, ETP expression was associated with aggressive mammary tumor growth and high metastatic growth. (jci.org)
  • Hence, the adipocyte, as a major constituent of the mammary tumor stroma ( 3 ), is a likely contributor to tumor growth. (jci.org)
  • Furthermore, the expression of IGF-1R may change in the course of time (e.g., because of tumor growth and therapy). (snmjournals.org)
  • Because of its mutated aggressive genetics, this cell has a selective growth advantage over its neighbors. (medscape.com)
  • In the present study, the relation between deoxycytidine kinase (dCK) activity and the radiosensitising effect of gemcitabine was investigated in eight different human tumour cell lines. (biomedcentral.com)
  • Sulfated glucuronosyl glycosphingolipids (SGGLs) are cell surface molecules that are endowed with the Human Natural Killer-1 (HNK-1) carbohydrate epitope. (karger.com)
  • The paternal cell lines are (1) the p53-wt (wild type) pluripotent gonadal germ cell tumor cell line NTERA-2, (2) the p53-mutated pluripotent extragonadal nonseminomatous germ cell tumor cell line NCCIT and (3) the p53-wt nullipotent embryonal cell carcinoma 2102EP, derived from a primary human testicular teratocarcinoma. (biomedcentral.com)
  • We utilized a quantitative RT-PCR (RTQ-PCR) based platform for detection of almost all currently known (738) human micro-RNA species on these 6 cell lines. (biomedcentral.com)
  • The anti‐proliferative activity of all newly synthesized compounds has been assessed against six human solid tumor cell lines. (researchgate.net)
  • All newly synthesized compounds were screened for anti‐proliferative activity against different human tumor cell lines. (researchgate.net)
  • Compound 5 showed high antiproliferative activity against several human tumor cell lines. (researchgate.net)
  • This technologic and biologic revolution continued through the 1960s to today, and the explosion in technology has fueled the current expansion of knowledge into the working of the human cell. (medscape.com)
  • Among human TEs, LINE-1s is the most abundant class. (lu.se)
  • We have in preliminary analysis found that LINE-1s are highly expressed in human glioma. (lu.se)
  • To investigate how LINE-1s influence human glioma, we will generate a transcriptomic and epigenomic atlas of LINE-1s in glioma resections at single-cell resolution, by combining multi-omics and long-read sequencing with unique bioinformatic tools. (lu.se)
  • The IFA method used in most laboratories uses human tumor cell-line substrate (the HEp-2 cell line) to detect the presence of these antibodies in human serum. (medscape.com)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
  • We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
  • Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
  • However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
  • Although possible, these strategies are often complicated to transfer to human cells due to technical issues (9 ), and only a few successful cases have been described ( 10, 11 ). (lu.se)
  • Here we describe how this system can be used for human embry- onic stem cells. (lu.se)
  • As these kinds of health effects data become available and methods to assess levels of significant human exposure improve, these MRLs will be revised. (cdc.gov)
  • Other treatment options include: hematopoietic stem cell transplantation, intensity-modulated radiation therapy, radiofrequency ablation, stereotactic body radiation therapy, intraperitoneal hyperthermic chemoperfusion, and clinical trials. (wikipedia.org)
  • A controlled clinical crossover trial of exercise training to improve cognition and neural communication in pediatric brain tumor survivors. (scielo.br)
  • To tailor CRC treatment, it is essential to first model this heterogeneity by defining subtypes of patients with homogeneous biological and clinical characteristics and second match these subtypes to cell lines for which extensive pharmacological data is available, thus linking targeted therapies to patients most likely to respond to treatment. (tudelft.nl)
  • Background Anti(α)-programmed cell death-1 (PD-1)/programmed death-ligand 1 (PD-L1) monotherapy fails to provide durable clinical benefit for most patients with carcinoma. (bmj.com)
  • To obtain an independent test set, the remaining 11 of the 58 tumors were selected from an ongoing clinical trial and used here as a blinded sion via ER,3 however the details of the estrogen effect on down- test set. (lu.se)
  • Understanding how HSPCs are regulated is of central importance for several areas in clinical hematology, including stem cell transplantation and the treatment of leukemias. (lu.se)
  • It is widely accepted that the cell origin for NB arises from the sympathoadrenal lineage of the neural crest during development [ 3 ]. (jcancer.org)
  • The neural crest is present during embryogenesis and gives rise to diverse cell types including enteric neurons and glia, as well as peripheral neurons [ 3 ]. (jcancer.org)
  • In addition, we have analyzed serum antibody titers against SGPG, GD3, and OAc-GD3 in patients with neural tumors by ELISA and HPTLC immunostaining. (karger.com)
  • Patients are often young and healthy as the tumors grow and spread uninhibited within the abdominal cavity. (wikipedia.org)
  • This is an aggressive, rare, fast spreading tumor and both pediatric and adult patients should be treated at a sarcoma center. (wikipedia.org)
  • Background Adoptive T cell transfer (ACT) therapy improves outcomes in patients with advanced malignancies, yet many individuals relapse due to the infusion of T cells with poor function or persistence. (bmj.com)
  • Toll-like receptor (TLR) agonists can invigorate antitumor T cell responses when administered directly to patients, but these responses often coincide with toxicities. (bmj.com)
  • Methods: Magnetic resonance images of 30 patients with TSC were evaluated. (eur.nl)
  • All sera had high titers of antibodies of the IgM isotype against SGPG (titers over 1:3,200), especially in tumors such as meningiomas, germinomas, orbital tumors, glioblastomas, medulloblastomas, and subependymomas. (karger.com)
  • Antinuclear antibody (ANA) tests identify antibodies present in serum that bind to autoantigens present in the nuclei of mammalian cells. (medscape.com)
  • However, this technique cannot be used to measure the expression in different tumor lesions and different regions within a tumor, because this would require multiple invasive procedures. (snmjournals.org)
  • Cerebellar lesions and subependymal giant cell tumors were counted. (eur.nl)
  • Participants of the cohort Pediatric Brain Tumor Atlas: CBTTC (CBTTC cohort), were randomly divided into training and validation cohorts. (scielo.br)
  • Pediatric brain tumor genetics: what radiologists need to know. (scielo.br)
  • Executive dysfunction in pediatric posterior fossa tumor survivors: a systematic literature review of neurocognitive deficits and interventions. (scielo.br)
  • H-29 cells were used for in vitro experiments in this study. (wjgnet.com)
  • We created an in vitro model of acquired cisplatin resistance by long term exposure of three well established germ cell tumor cell lines to cisplatin, resulting in sublines with significantly increased resistance to cisplatin. (biomedcentral.com)
  • However, distinct tumors differentially regulated this inherent MO-MDSC differentiation program, indicating that this phenomenon was tumor driven. (ashpublications.org)
  • Pharmacological response data for targeted inhibitors of SRC, WNT, GSK3b, aurora kinase, PI3 kinase, and mTOR, showed significant differences in sensitivity across cell lines assigned to different subtypes. (tudelft.nl)
  • Thus, although initially thought to be of mesothelial origin due to sites of presentation, it is now hypothesized to arise from a progenitor cell with multiphenotypic differentiation. (wikipedia.org)
  • The hsa-miR-371-373 cluster (counteracting cellular senescence and linked with differentiation potency), as well as hsa-miR-520c/-520h (inhibiting the tumor suppressor p21) were 3.9-16.3 fold up-regulated in two of the three cisplatin resistant cell lines. (biomedcentral.com)
  • We compared microRNA expression patterns in three cisplatin resistant sublines derived from paternal cisplatin sensitive germ cell tumor cell lines in order to improve our understanding of the mechanisms of cisplatin resistance. (biomedcentral.com)
  • Three cisplatin resistant sublines (NTERA-2-R, NCCIT-R, 2102EP-R) showing 2.7-11.3-fold increase in drug resistance after intermittent exposure to increasing doses of cisplatin were compared to their parental counterparts, three well established relatively cisplatin sensitive germ cell tumor cell lines (NTERA-2, NCCIT, 2102EP). (biomedcentral.com)
  • Our approach was designed to characterize the role of micro-RNAs on the presumably multifactorial phenomenon of acquired cisplatin resistance in germ cell tumors. (biomedcentral.com)
  • Originally described by Headington in the 1970s, trichoblastoma (TBL) is now appreciated as a rare benign tumor that originates from the follicular germ cell line [1, 2]. (karger.com)
  • SVC112 reduced sphere formation by cell lines and CSCs. (nih.gov)