Coupled to cyanogen bromide activatedAgaroseChlorideBrCNAffinity gelsSodiumCompoundsAntibodyToxicityLigandBeadsProceedsNIOSHSulfurAntigenInvolvesCarbonProteinsDerivativesProteinWaterChemicalPurificationSamplesAnalyticalComparisonLaboratoryMaterialMolecularFormStudiesPreviouslyHeatResultsActivated gel
Coupled to cyanogen bromide activated1
- Alfa-amylase was coupled to cyanogen bromide activated Sephadex G200 at pH8.3 and 7.0. (who.int)
Agarose4
- Cyanogen bromide is often used to immobilize proteins by coupling them to reagents such as agarose for affinity chromatography. (wikipedia.org)
- Cyanogen bromide is also often used because it reacts with the hydroxyl groups on agarose to form cyanate esters and imidocarbonates. (wikipedia.org)
- Also, cyanogen bromide activation involves the attachment of a ligand to agarose by an isourea bond, which is positively charged at neutral pH and thus unstable. (wikipedia.org)
- Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method. (abcam.cn)
Chloride13
- Cyanogen chloride (CK) is a highly volatile and toxic chemical asphyxiant that interferes with the body's ability to use oxygen. (cdc.gov)
- Exposure to cyanogen chloride (CK) can be rapidly fatal. (cdc.gov)
- Cyanogen chloride (CK) has strong irritant and choking effects. (cdc.gov)
- Cyanogen chloride (CK) is a chemical warfare agent (military designation CK). (cdc.gov)
- Indoor Air: Cyanogen chloride (CK) can be released into indoor air as a liquid spray (aerosol) or as a gas. (cdc.gov)
- Water: Cyanogen chloride (CK) can be used to contaminate water. (cdc.gov)
- Agricultural: If cyanogen chloride (CK) is released into the air as a liquid spray (aerosol), it has the potential to contaminate agricultural products. (cdc.gov)
- If cyanogen chloride (CK) is released as a gas, it is highly unlikely to contaminate agricultural products. (cdc.gov)
- Cyanogen chloride (CK) can affect the body by inhalation, ingestion, skin contact, or eye contact. (cdc.gov)
- DBPs are formed by the reaction of natural organic matter (NOM) or inorganic substances in water (e.g. chloride, bromide) with the disinfectant chemical. (nunofsoares.com)
- Cyanogen chloride (CNCl or CK) is heavier and will sink to low-lying areas and increase the risk of exposure. (medscape.com)
- the other is cyanogen chloride (NATO designation CK). (medscape.com)
- The introduction of cyanogen chloride by the French in 1916 made available a compound that, being both more toxic and less volatile, was a more effective chemical agent. (medscape.com)
BrCN2
- Cyanogen bromide is the inorganic compound with the formula (CN)Br or BrCN. (wikipedia.org)
- Like some other cyanogen compounds, cyanogen bromide undergoes an exothermic trimerisation to cyanuric bromide ((BrCN)3). (wikipedia.org)
Affinity gels2
- Because of its simplicity and mild pH conditions, cyanogen bromide activation is the most common method for preparing affinity gels. (wikipedia.org)
- For the final step, several affinity gels were tested and the 1 containing cycloserine coupled to CNBr [cyanogen bromide]-activated Sepharose-4B was selected. (eurekamag.com)
Sodium3
- The (±)-cis isomers [racemate (1:1-mixture) of the (4R,5S)-isomer and the enantiomeric (4S,5R)-isomer] generally synthesized from dl-phenylpropanolamine in one step by cyclization with cyanogen bromide (sometimes prepared in situ by reacting sodium cyanide with bromine). (psychonautwiki.org)
- Alternate synthesis routes generally involve more steps, such as replacing cyanogen bromide with sodium or potassium cyanate to form an intermediate and then reacting it with concentrated hydrochloric acid. (psychonautwiki.org)
- Among them, the mechanochemical method with percarbonamide as the oxidant and sodium hexametaphosphate as the grinding aid has the best effect of activating peat. (researchgate.net)
Compounds1
- In addition, a number of cyanide-containing compounds, known as cyanogens, may release cyanide during metabolism. (medscape.com)
Antibody1
- An immunoaffinity chromatography matrix was prepared by coupling antibody to cyanogen bromide-activated Sepharose. (waikato.ac.nz)
Toxicity1
- The disadvantages of this approach include the toxicity of cyanogen bromide and its sensitivity to oxidation. (wikipedia.org)
Ligand2
- One method we have used to study ligand binding is to incorporate the photo-reactive crosslinker p-benzoyl-L-phenylalanine (Bpa) into Ste2p to capture alpha-factor. (tennessee.edu)
- Another method to study ligand binding that we have adapted for use in our system is to incorporate a chemical crosslinker [3,4-dihydroxylphenylacetyl (DHPA)] into alpha-factor for periodate-mediated crosslinking to Ste2p. (tennessee.edu)
Beads1
- This vaccine was prepared from the C. parvum oocysts antigen using immune affinity chromatography with cyanogen bromide-activated Sepharose-4B beads. (bvsalud.org)
Proceeds1
- The cyanate reaction proceeds differently from the cyanogen bromide and transforms norephedrine into trans-4-methylaminorex instead. (psychonautwiki.org)
NIOSH1
- NIOSH manual of analytical methods [online]. (cdc.gov)
Sulfur2
- If the sulfur in cysteine attacked cyanogen bromide, the bromide ion would deprotonate the cyanide adduct, leaving the sulfur uncharged and the beta carbon of the cysteine not electrophilic. (wikipedia.org)
- series and site of all sulfur of research methods, relying the media, paralympics, men and much news areas, without place of the use activities explores born. (dl-mirror-art-design.de)
Antigen1
- an evalutation of some methods of assay of foot-and-mouth disease antigen for vaccines. (liverpool.ac.uk)
Involves2
- The method involves expressing the recombinant polypeptide as a fusion protein with a pro-peptide. (justia.com)
- Conversion of this insoluble material involves often slow and complex refolding methods, making protein purification difficult. (justia.com)
Carbon3
- The carbon atom in cyanogen bromide is bonded to bromine by a single bond and to nitrogen by a triple bond (i.e. (wikipedia.org)
- The electron density in cyanogen bromide is shifted away from the carbon atom, making it unusually electrophilic, and towards the more electronegative bromine and nitrogen. (wikipedia.org)
- Corrective methods are oriented to reduce DBPs that are already formed (e.g. granular activated carbon adsorption). (nunofsoares.com)
Proteins1
- HCN + HOBr}}} The main uses of cyanogen bromide are to immobilize proteins, fragment proteins by cleaving peptide bonds, and synthesize cyanamides and other molecules. (wikipedia.org)
Derivatives1
- Characterization of prepared derivatives showed that both methods lead to formation of crosslinked humic structures. (researchgate.net)
Protein3
- The amounts of protein in the immobilized Alfa-amylase prepared at pH 8.3 and 7.0 were estimated by, Kjeldhal method, by the tryptophan content and from the difference in the amount of protein present in the original solution and that in the washings. (who.int)
- Comparison of the amino acid sequences of cyanogen bromide cleaved isomerase with the known sequence of the peroxisomal bifunctional protein from the rat identified them as the same molecule. (rhea-db.org)
- In control experiments, the peroxisomal bifunctional protein purified according to published methods also catalyzed delta 3, delta 2-enoyl-CoA isomerization. (rhea-db.org)
Water3
- 1998. Standard methods for the examination of water and wastewater. (cdc.gov)
- This method was previously developed for the characterization of water in polysaccharides 41−43 and also tested for humic acids. (researchgate.net)
- Less than or equal to 9.53 x 10 -6 g/L of solution at 20.0 ± 0.5 °C, using the flask method, designed to be compatible with Method A.6 Water Solubility of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 105 of the OECD Guidelines for Testing of Chemicals, 27 July 1995. (europa.eu)
Chemical3
- Mechanochemical method is an efficient processing method that uses mechanical means to change the structure and physical and chemical properties of objects. (researchgate.net)
- Predicted negative based on the chemical structure of the test item, using a procedure designed to be compatible with Method A.14 Explosive Properties of Commission Regulation (EC) No 440/2008 of 30 May 2008. (europa.eu)
- Selected analytical methods: chemical methods query [online]. (cdc.gov)
Purification1
- It is activated by mechanochemical method, and then humic acid and fulvic acid are extracted by alkali-soluble acid purification technology. (researchgate.net)
Samples1
- Materials and Methods Tissues Samples and Major Dental Cell Civilizations All studies had been carried out beneath the acceptance of the pet moral committee for the College or university of Alabama at Birmingham as well as the College or university of Texas Wellness Science Middle at San Antonio. (fsu93.org)
Analytical4
- routine analysis of suspensions of foot-and-mouth disease virus and eluates of vaccine by the isopycnic method in analytical ultracentrifuge demonstrates the important heterogeneity of the viral population. (liverpool.ac.uk)
- routine titration of foot-and-mouth disease virus suspensions by analytical ultracentrifugation 2nd communication: sedimentation equilibrium method. (liverpool.ac.uk)
- this method was based on sedimentation equilibrium in the analytical ultraviolet scanning ultracentrifuge. (liverpool.ac.uk)
- Sampling and analytical methods [online]. (cdc.gov)
Comparison1
- The cyanogen bromide, by comparison, transformed norephedrine into the cis isomer and norpseudoephedrine into the trans isomers of the final product. (psychonautwiki.org)
Laboratory2
- Supporting activities include the development of epidemiological, experimental laboratory, and risk-assessment methods that could produce internationally comparable results, and the development of manpower in the field of toxicology. (inchem.org)
- Food: Laboratory methods [online]. (cdc.gov)
Material1
- Phytase is an enzyme (phosphatase) that increases the Material and methods bioavailability of phosphorous of plant origin in Study group animal feeds. (cdc.gov)
Molecular1
- The mechanochemical methods can increase the molecular condensation degree and molecular relative mass of humic acid, but reduce the molecular condensation degree and molecular relative mass of fulvic acid. (researchgate.net)
Form1
- Preventive methods look for reducing the probability to form DBPs (e.g. reduction of NOM, use of alternative disinfectants, enhanced coagulation, etc. (nunofsoares.com)
Studies1
- ATTYGALLE, AB, Studies on immobilization of alfa- amylase to cyanogen bromide activated sephadex g 200, University of Sri Lanka (Colombo Campus) UC(MED), 1977: 50p. (who.int)
Previously2
- Human major oral cell populations had been Rabbit polyclonal to PLA2G12B set up from extracted tooth (attained with up to date consents) using previously released methods (MacDougall et al. (fsu93.org)
- Several previously unrecognized DS domains could be identified by this method, including the first examples from prokaryotic species. (embl-heidelberg.de)
Heat2
- A prepurification was performed by heat treatment, gel chromatography and DEAE-Sepharose methods. (eurekamag.com)
- outreach has effectively a Greek heat phrase( CCP) in method, issued to guide ygton2811October, search agriculture and kind part. (die4freis.de)
Results1
- The results show that mechanochemical the method can improve the extraction rate of humic acid and fulvic acid in peat. (researchgate.net)
Activated gel1
- The volume of the cyanogen bromide activated gel, at this pH decreased by about 50 percent, compared to that of the unactivated Sephadex G200. (who.int)