The high interest in improved diagnostic, prognostic and therapy control procedures in medicine as well as cellular aspects in immunology, cancer research, molecular biology, biotechnology and microbiology is concurrent with the development of cytometric assays for a variety of biochemically specific cell functions and markers, of instrumentation for flow and image cytometry and of software for cytometric multiparameter data processing. (purdue.edu)
Flow cytometric analysis demonstrated the high purity of generated microsamples. (researchgate.net)
Analyze3
IFC) is a technology that can automatically image and analyze processed blood samples for markers of radiation damage, the microscope.based biodosimetry techniques can be modified for the IFC for high.throughput biological dosimetry. (genome-integrity.org)
However, such research is currently limited by a lack of a systematic mathematical framework to adequately model and analyze time-series flow cytometry data. (biomedcentral.com)
Diagnosis3
A lymphoma diagnosis often includes imaging tests. (healthline.com)
This innovative diagnostic technique has the potential to transform cancer diagnosis by enabling the development of non-invasive or minimally invasive methods for detection, prognosis, and monitoring of cancer patients. (ethz.ch)
We evaluated characteristics of the bone marrow mast cells by pathology, flow cytometry and detection of D816V mutation by using current WHO-criteria, which led to changes in the final diagnosis compared to the assessments done by classical allergy work-up and measurements of sBT. (biomedcentral.com)
Examine2
Multiplex imaging has emerged as an invaluable tool for immune-oncologists and translational researchers, enabling them to examine intricate interactions among immune cells, stroma, matrix, and malignant cells within the tumor microenvironment (TME). (bmj.com)
To address this, we used flow cytometry to examine changes in the temporal expression of microglia and macrophages after SCI and intravenous morphine. (biomedcentral.com)
Markers2
Modern flow cytometry technology has enabled the simultaneous analysis of multiple cell markers at the single-cell level, and it is widely used in a broad field of research. (biomedcentral.com)
Modern flow cytometers allow the simultaneous detection of nearly 20 protein markers per cell with a throughput of thousands of cells per second. (biomedcentral.com)
Throughput1
While these assays can be very sensitive and specific, the standard methods need to be adapted to increase sample throughput in the case of a large.scale radiological/nuclear event. (genome-integrity.org)
Results2
In this study, we compare the results of different biodosimetry methods on the same irradiated cells to assess the suitability of current biomarkers and push forward the idea of employing a multiparametric approach to achieve an accurate dose and risk estimation. (genome-integrity.org)
Our results indicate that CYBERTRACK offers better understandings of time-dependent cell population dynamics to cytometry users by systematically analyzing time-series flow cytometry data. (biomedcentral.com)
Inflammation1
METHODS: The lung disease specificity of OPN and epithelial inflammation were defined by bioinformatics. (bvsalud.org)
Cells8
The application of both dynamic and static imaging methods over the past two decades has revealed a fundamental aspect of immune function, namely, the key role for the spatial organization of innate and adaptive immune cells within a tissue in mediating effective host defense or producing pathology. (bmj.com)
An important challenge in the analysis of flow cytometry data is the classification of individual cells into canonical cell types, that is, subset populations such as T and B cells. (biomedcentral.com)
The formation of new vessels in the hypoxic area restores blood flow, provides oxygen and nutriments to the surviving cells, and promotes the migration and engraftment of new cells. (elifesciences.org)
Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber. (justia.com)
In particular, the invention relates to microfluidic devices and their uses and methods for assaying cells. (justia.com)
Similarly, single cell methods are vital in stem cell research and cancer biology, where isolated populations of primary cells are heterogeneous due to limitations in purification protocols, and it is often a minority cell population that is the most relevant. (justia.com)
Existing methods for measuring transcript levels in single cells include RT-qPCR (1), single molecule counting using digital PCR (2) or hybridization probes (3, 4), and next generation sequencing (5). (justia.com)
The current study firstly suggested that NFAT2 suppressed the aggression and malignancy of HepG2 cells through inducing the expression of Egr2. (researchsquare.com)
Analysis2
In this review, our aim is to present an overview of the utilization of multiplex imaging in immuno-oncology studies and inform novice researchers about the fundamental principles at each stage of the imaging and analysis process. (bmj.com)
Time-series flow cytometry data are indispensable for investigating the dynamics of cell populations that could not be elucidated by static time-point analysis. (biomedcentral.com)
Measurements2
In order to ensure high fidelity performance, we have implemented a high-speed microscope for in-process quality control, and image-based confluence measurements for automated dilution ratio calculation. (frontiersin.org)
All existing data (satellite images, variability of winds,submarine measurements) say this a highly variable system", said Bindschadler. (nasa.gov)
Lung cancer1
Current treatment methods for lung cancer are mostly traditional methods, including surgery, chemotherapy, and radiotherapy. (immune-source.com)
Data7
The detection of cell populations in flow cytometry data has long been dependent on "manual gating" by visual inspection. (biomedcentral.com)
We propose a simple and efficient statistical framework, named CYBERTRACK (CYtometry-Based Estimation and Reasoning for TRACKing cell populations), to perform clustering and cell population tracking for time-series flow cytometry data. (biomedcentral.com)
CYBERTRACK assumes that flow cytometry data are generated from a multivariate Gaussian mixture distribution with its mixture proportion at the current time dependent on that at a previous timepoint. (biomedcentral.com)
Although these methods have been successfully applied to identify both major and rare cell populations, they are not designed for modeling and analyzing time-series data and thus cannot capture the time-dependent properties and dynamics of cell populations. (biomedcentral.com)
Time-series flow cytometry data offer information on longitudinal cell population dynamics that could not be elucidated by conventional static time-point data. (biomedcentral.com)
To address this problem, we propose a new statistical framework, named CYBERTRACK (CYtometry-Based Estimation and Reasoning for TRACKing cell populations), for the automatic clustering and tracking of a mixture proportion of cell populations in time-series flow cytometry data. (biomedcentral.com)
Cell2
Flow cytometry is a widely used technology for identifying and quantifying cellular properties and cell populations by measuring expression levels of surface and intracellular proteins at the single-cell level. (biomedcentral.com)
The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. (justia.com)
Treatment1
and (2) discover new targets to overcome current treatment limitations. (bmj.com)
Organs2
During an ultrasound , sound waves bounce off tissues and organs to create an image. (healthline.com)
At both high (x ray) and low (radio frequency [RF]) energies the body is mostly transparent, thus allowing the non-invasive imaging of the internal structure of organs and bones. (nationalacademies.org)
Studies1
Our flow cytometry studies showed that 3 consecutive days of morphine administration after an SCI significantly increased the number of microglia and macrophages around the lesion. (biomedcentral.com)
Chronic1
Current understanding characterizes IA as a chronic inflammatory disease. (biomedcentral.com)
Model1
Here, we detail and model a blood separation principle which exploits discrete viscosity differences caused by blood particle sedimentation in a laminar flow. (researchgate.net)
Blood flow2
median age, 65 years) by whole-blood flow cytometry and Enzyme-linked immunosorbent assay (ELISA), respectively (326 specimens). (nature.com)
The amount of this intrinsic absorption can be used to determine the physiological health of an organ-for example, whether the tissue is getting sufficient oxygenated blood flow. (nationalacademies.org)
Study1
6 Multiplex immunoimaging has emerged as one important step in this direction and this review succinctly describes contemporary study design considerations and experimental approaches associated with such imaging. (bmj.com)
Western1
The iPSC lines were differentiated toward mature cortical neurons, and AD pathological hallmarks were analyzed by RT-qPCR, enzyme-linked immunosorbent assay, and Western blotting methods. (biomedcentral.com)
High3
Hettich M, Braun F, Bartholomä MD, Schirmbeck R, Niedermann G. High-Resolution PET Imaging with Therapeutic Antibody-based PD-1/PD-L1 Checkpoint Tracers. (thno.org)
We developed radiotracers based on therapeutic checkpoint-blocking antibodies permitting sensitive and high-resolution PET imaging of both PD-1 and PD-L1 in immunocompetent mice. (thno.org)
Despite the availability of analytical methods, the difficulty in obtaining high-quality and standardized microsamples at the point of collection remains a major limitation of the process. (researchgate.net)
Performance2
The CYBERTRACK performance is validated using two real flow cytometry datasets, which demonstrate that the population dynamics detected by CYBERTRACK are consistent with our prior knowledge of lymphocyte behavior. (biomedcentral.com)
This work describes the performance of a modified charge integration discrimination method developed to test the viability of the new design. (spie.org)
Research1
The project aims to determine the underlying causesbehind why Pine Island Glacier has begun to flow more rapidly and dischargemore ice into the ocean," saidScott Borg, director of NSF's Division of Antarctic Sciences, the group thatcoordinates all U.S. research in Antarctica on the southernmost continent and surrounding oceans. (nasa.gov)
Samples2
Proteomic and metabolomic analyses of the microsamples of 283 proteins and 1351 metabolite features was consistent with samples generated via a conventional centrifugation method. (researchgate.net)
was consistent with samples generated via a conventional centrifugation method. (researchgate.net)
Paper1
Current state requisition forms, outlook or paper calendars will not be in use. (masseyeandear.org)