To investigate the pharmacokinetics of arotinoid acid, a sensitive and selective liquid chromatographic-tandem mass spectrometric method (LC-MS/MS) for the determination of arotinoid acid in human plasma was developed and validated. (unboundmedicine.com)
Surprisingly, bacteria in the stationary phase produced N-acyl-phosphatidylethanolamine (NAPE) and another rare lipid we tentatively name as 1-phosphatidyl-2-acyl-glycero-3-phosphoethanolamine (PAGPE) based on tandem mass spectrometry. (nature.com)
Extracts were analyzed by a liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method, which was developed and validated for this study. (uzh.ch)
Creatinine measurements in 24 h urine by liquid chromatography-tandem mass spectrometry. (cdc.gov)
A simple, sensitive, and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determining urinary creatinine was developed and used to evaluate 24 h urine samples collected during an exposure study. (cdc.gov)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is increasingly utilized in clinical laboratories because it has advantages in terms of specificity and sensitivity over other analytical technologies. (annlabmed.org)
Qualitative analyses of the compounds were performed in full mass spectrometry/data-dependent tandem mass spectrometry (MS/dd-MS2) mode. (bvsalud.org)
Tandem quadrupole mass spectrometry HPLC-ESI-MS/MS CID-MS/MS Multiple reaction monitory (MRM) Quercetin Apigenin Kaempferol a b s t r a c t The presence of three flavonols (quercetin, kaempferol, myricetin) and two flavones (apigenin, luteolin) were investigated in the extract of fresh spinach leaves. (utc.fr)
Furthermore, the results were confirmed by HPLC coupled to an electrospray ionisation tandem triple-quadrupole mass spectrometer ESI-MS performing low-energy collision induced dissociation (CID-MS/MS) in the collision cell (HPLC-ESI-MS/MS). Analyses were made in the multiple reaction monitory (MRM) mode. (utc.fr)
Caffeine and 14 of its metabolites are quantified in urine by use of high-performance liquid chromatography-electrospray ionization-tandem quadrupole mass spectrometry (HPLC-ESI-MS/MS) with stable isotope labeled internal standards. (cdc.gov)
In this light, assembling and chelating properties of sodium bis(2-ethylhexyl)sulfosuccinate (AOTNa) towards divalent metal ion chlorides have been investigated in the gas phase by electrospray ionization mass spectrometry in negative ion mode, tandem mass spectrometry and energy-resolved mass spectrometry. (unipa.it)
Finally, PFASs in D. magna were extracted using an ion-pairing agent and analyzed through liquid chromatography-tandem mass spectrometry. (american-jiras.com)
Tandem mass spectrometry analysis of plasma from obese mice under CR showed no difference in Cur metabolite levels between groups supplemented with Cur alone or combined with Pip. (springer.com)
FFPE tissue slides were scanned and analyzed digitally, while myocardial proteins were quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using isobaric labeling. (biomedcentral.com)
Aqueous3
The glucosinolates were extracted with hot methanol/water (70:30 v/v) and separation of the individual glucosinolates was achieved using a reversed-phase C18 column with an aqueous ammonium acetate/methanol gradient. (unboundmedicine.com)
The dry residue was dissolved with 50% methanol aqueous solution. (bvsalud.org)
The antioxidant activity of fresh tomatoes, tomato paste, and three fractions obtained from these products (i.e., aqueous, methanol, and hexane fractions) was also determined. (scite.ai)
Formic acid4
SPE columns were eluted with 2% formic acid in methanol. (cdc.gov)
Samples are then acidified with 30 µL of 2.0 N HCl and 250 µL of a 1:9 methanol/water solution containing 0.1% formic acid such that the matrix of the sample is similar to the starting mobile phase composition of the analysis step. (cdc.gov)
Prepare the metabolic extracts of the berry samples at room temperature in three volumes of methanol, acidified with 0.1%formic acid, in an ultrasonic bath at 40 kilohertz for 15 minutes. (jove.com)
Chromatographic separations were performed using a gradient program with a mixture of water (0.05% formic acid), as mobile phase A and acetonitrile (0.05% formic acid), as mobile phase B. Analytes quantification was performed by electro-spray ionisation-single quadrupole mass spectrometry using the selected ion recording (SIR) detection mode. (unito.it)
Triple quadrupole1
An isotope dilution mass spectrometry based method (LC-MS/MS triple quadrupole MS using electrospray ionization) was used for routine quantitation of AFB-lys as a measure of individual exposure to aflatoxin B1. (cdc.gov)
Quadrupole2
In this study, a method based on high performance liquid chromatography-quadrupole/orbitrap mass spectrometry was established for the simultaneous determination of 22 mycotoxins in milk. (bvsalud.org)
The analytes were determined by liquid chromatography-quadrupole/orbitrap mass spectrometry in positive electrospray ionization mode. (bvsalud.org)
Liquid7
An optimized method using liquid chromatography coupled with electrospray ionization ion trap mass spectrometry (LC/ESI-ITMS) in negative ion mode has been developed for screening different structural classes of intact glucosinolates in six Chinese medicinal herbs. (unboundmedicine.com)
A specific liquid chromatography-mass spectrometry/mass spectrometry spectrometric procedure for the quantitation of amprenavir drug in biological matrices was developed and validated. (japsonline.com)
The literature on amprenavir unveiled that there are few analytical procedures available for the estimation of drug in API and dosage forms on liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) with more retention times. (japsonline.com)
2016) describe the quantitative method they used to analyze DHEA, AD and testosterone in human serum by stable-isotope dilution liquid chromatography-high-resolution mass spectrometry (LC-HRMS). (thermofisher.com)
2016) " Validation of highly sensitive simultaneous targeted and untargeted analysis of keto-steroids by Girard P derivatization and stable isotope dilution-liquid chromatography-high resolution mass spectrometry ," Steroids, 116 (pp. 60-66), doi: 10.1016/j.steroids.2016.10.003. (thermofisher.com)
A sensitive and accurate high performance liquid chromatography-mass spectrometric (HPLC-MS) method for the intracellular determination of 14 antiretroviral drugs in peripheral blood mononuclear cells (PBMCs) for HIV+ patients was validated. (unito.it)
We also present a novel method for characterization of the materials using liquid chromatography mass spectrometry to quantify the analytes of interest. (cdc.gov)
Extract3
The flavo-noids glycosides in the extract were hydrolysed to their aglycons with 1.2 M HCl in boiling 50% watermethanol solution. (utc.fr)
In particular, the methanol extract was the most effective in protecting neuron-like SH-SY5Y cells against H 2 O 2 -induced oxidative stress by upregulating endogenous antioxidant enzymes such as thioredoxin reductase, heme oxygenase 1, NADPH quinone oxidoreductase, and glutathione reductase. (hindawi.com)
The methanol extract was focused under decrease pressure to provide a deep dark brown syrup extract. (engineering-gdfsuez.com)
Ammonium acetate1
Chromatographic separation was achieved on a Zorbax Extend C(18) column (150mmx4.6mm, i.d., 5microm) using methanol:acetonitrile:5mM ammonium acetate (48:32:20, v/v/v) as the mobile phase at a flow rate of 0.8mL/min. (unboundmedicine.com)
Extracts1
This study was designed to evaluate the phytochemical composition, antioxidant, alpha-amylase, and tyrosinase inhibitory activity of ethyl acetate, methanol, and water extracts of Campanula macrostachya Waldst. (metu.edu.tr)
HPLC-ESI-MS1
Samples are filtered and analyzed by use of HPLC-ESI-MS/MS in both positive and negative ionization modes. (cdc.gov)
METHODS:Water/methanol solutions of AOTNa and chloride salts of nickel, magnesium, calcium and manganese, with different AOTNa/metal salt ratios, were infused into the electrospray source of a LCQ DECA ion trap mass spectrometer, operating in negative ion mode, at a flow rate of 5 mL/min. (unipa.it)
then processed the prepared samples on an UltiMate 3000 quaternary ultra-performance LC system (Thermo Scientific) equipped with a refrigerated autosampler (6°C) and a column heater (60°C) and coupled to a Q Exactive Plus HR mass spectrometer (Thermo Scientific). (thermofisher.com)
Constituents1
By using methanol/tetrahydrofuran as solvent, the constituents were identified on the basis of quasi-molecular ions. (degruyter.com)
Solvent1
using HSCCC, and examines the effect of the two-immiscible solvent system (TISS) composed of chloroform- methanol-0.2 mol/L hydrochloric acid (4:1.5:2). (iomcworld.com)
Analyses1
UV analyses for pure samples were recorded, separately, in methanolsolutions on UV spectra and were measured using a Shimadzu UV 240 spectrophotometer (P/N 240-58000). (hindawi.com)
Efficiency2
Before injecting the preparation, they added 20 μl of Girard's Reagent P (GP) to derivatize the keto groups of testosterone, AD and DHEA, to improve ionization efficiency using electrospray ionization. (thermofisher.com)
The performance of electrospray ionization is heavily dictated by spray-solution composition, which can alter ionization efficiency and pathways. (aip.org)
Method3
The results indicate the atmospheric-pressure ionization MS with electrospray ionization analysis is an effective method for the analysis of sucrose esters composed of different isomers. (degruyter.com)
The most common currently used method of analyzing for the presence of sulfur mustard and its metabolites in biological and environmental samples is gas chromatography/mass spectrometry (GC/MS). Prior to 1987, however, thin-layer chromatography (TLC) with a colorimetric detection system and gas chromatography with either flame ionization detector (FID), electron capture detector (ECD), or flame photometric detector (FPD) were the most frequently used methods. (cdc.gov)
We describe a novel method to measure petroleum distillates in BAL fluid using gas chromatography-mass spectrometry (GC/MS). METHODS: n-Hexane, n-heptane, n-octane, methylcyclopentane, and cyclohexane were measured in BAL fluid specimens by headspace solid-phase microextraction/gas chromatography/mass spectrometry. (cdc.gov)
Analyte2
Recently, solution-electrode glow discharges (SEGDs) have shown great utility as ionization sources for mass spectrometry (MS). The solution composition of SEGD electrodes is pivotal to their performance as it influences analyte-ion formation. (aip.org)
The changing composition of the SCGD solution alters analytical performance, but also provides insight into analyte ionization and fragmentation processes. (aip.org)
Analytical1
Acetonitrile (ACN) and methanol of LC grade and HCOOH of analytical grade were procured from JT Bakers, Ahmedabad, India. (japsonline.com)
Reaction1
The detection was carried out in multiple reaction monitoring (MRM) mode via negative electrospray ionization (ESI) interface. (unboundmedicine.com)
Specimens1
Nail specimens were pulverized and extracted with methanol (internal standard: zolpidem-D6) under sonication. (uzh.ch)
Serum1
The investigators added an internal standard solution containing 10 pg/μl testosterone, 10 pg/μl AD and 50 pg/μl DHEA in methanol (20 μl) to 100 μl samples of double-charcoal stripped serum or serum. (thermofisher.com)
Water1
Extraction of drugs from PBMCs pellets was obtained with methanol:water (70:30, v/v), with quinoxaline added as internal standard, after a sonication step. (unito.it)
Characterization2
The rapid and highly sensitive atmospheric-pressure ionization MS with electrospray ionization analysis technique proposed here is well suited for direct characterization of sucrose esters. (degruyter.com)
Characterization and Purification The whole-plants of had been air-dried, powdered, and extracted with methanol under reflux. (engineering-gdfsuez.com)
Source5
In addition, P. pastoris uses a specific set of inducible enzymes to assimilate methanol as the sole carbon and energy source. (biomedcentral.com)
Mut S strains still express Aox2 but grow slower than wild type strains when methanol is used as the sole carbon source. (biomedcentral.com)
Ultrahigh-resolution mass spectrometry of DOM in bottled waters and their corresponding source waters identified thousands of molecular formulae characteristic of mostly refractory, soil-derived DOM. (biomedcentral.com)
An LC-MS/MS SCIEX API4000 instrument, furnished with a positive electrospray ionization source and HPLC of Shimadzu, consisted of an SIL-HTC autosampler, dual pump, and column oven, which were utilized in the present work. (japsonline.com)
The impact of several additives to the acidic solution of an SCGD ionization source was evaluated based on mass-spectrometric performance. (aip.org)
Molecules1
The photoisomerisation of charged molecules in solution is probed directly using ion mobility mass spectrometry with electrospray ionisation. (uea.ac.uk)
Detection2
After production, detection again is by mass-spectroscopic means. (wikipedia.org)
To test this hypothesis, we used a rat model of endurance exercise, a microwave irradiation for accurate detection of glycogen in the brain (the cortex, hippocampus, and hypothalamus), and capillary electrophoresis mass spectrometry-based metabolomics to observe the comprehensive metabolic profile of the blood. (frontiersin.org)
Solution2
While SEGDs produce Taylor cones similar to electrospray ionization, the influence of solution-electrode composition on molecular-ion formation has not been studied. (aip.org)
Here, we examine how additives to an atmospheric-pressure solution-cathode glow discharge (SCGD) influence molecular ionization and fragmentation. (aip.org)
Results1
Electrospray ionization of an aerosol of serine in methanol results in a mass spectrum with a prominent ion peak of 841 corresponding to the Ser8+H+ cation. (wikipedia.org)
Directly1
Mass spectrometry imaging was able to differentiate the tumor distribution of co-dosed deuterated non-nanoparticle-formulated free drug alongside the nanoparticle-formulated drug by directly visualizing both delivery approaches within the same animal or tissue. (thno.org)
Lower2
This reduction in product quality could outweigh the advantages of using Mut strains, such as lower oxygen and methanol demand, heat formation and in some cases improved expression. (biomedcentral.com)
The slow growth and lower methanol consumption of Mut S strains may have some advantages at large scale processes such as a lower demand for oxygen and reduced heat formation [ 6 , 7 ]. (biomedcentral.com)
Multiple1
By staining for multiple biomarkers of the tumor microenvironment on the same tumor sections using imaging mass cytometry, co-registering and integrating data from both imaging modalities it was possible to determine the features in regions with highest nanomedicine distribution. (thno.org)