Metastatic tumor cells cultured. Medical search. Frequent questions

CSCs are enriched near macrophages, particularly near macrophage-containing intravasation sites called Tumor Microenvironment of Metastasis (TMEM) doorways. (nature.com)
Tanner joined the National Cancer Institute as a Stadtman Tenure-Track Investigator in July 2012, where she integrates concepts from molecular biophysics and cell biology to learn how cells and tissues sense and respond to their physical microenvironment, and to thereby design therapeutics and cellular biotechnology. (wikipedia.org)
On the other hand, intercellular transfer of mitochondria and cellular components via extracellular vesicles (EVs) and tunneling nanotubes (TNTs) has recently attracted intense attention in terms of cell-to-cell communication in the tumor microenvironment. (biomedcentral.com)
In this study, we investigated whether mtDNA with the NADH dehydrogenase subunit 6 ( ND6 ) G13997A pathogenic mutation in highly metastatic cells can be horizontally transferred to low-metastatic cells and stromal cells in the tumor microenvironment. (biomedcentral.com)
These results suggest that metastasis-enhancing pathogenic mtDNA derived from metastatic tumor cells is transferred to low-metastatic tumor cells and stromal cells via S-EVs in vitro and in the tumor microenvironment, inferring a novel mechanism of enhancement of metastatic potential during tumor progression. (biomedcentral.com)
and they can deplete the tumor microenvironment of nutrients necessary for cancer cell survival. (aacr.org)
3D cell culture methods - and multicellular tumor spheroids (MCTS), in particular - are quickly becoming a valuable tool for studying the tumor microenvironment in cancer research . (corning.com)
Cancer scientists are creating increasingly complex tumor models that more accurately replicate in vivo conditions - including the tumor microenvironment, which plays a key role in tumor progression, according to a study published in BioTechniques . (corning.com)
modeling it in the tumor microenvironment, though, has been challenging. (corning.com)
Mouse tumor inoculation models revealed that the tumor-forming capabilities of OS5K-3 ENG + and ENG - cells in vivo were highly dependent on the microenvironment, with the renal microenvironment most preferable to ENG + cells. (nih.gov)
In conclusion, the renal microenvironment, rather than the hypothesized ENG + cell-centered hierarchy, maintains cellular heterogeneity in clear cell renal cell carcinoma. (nih.gov)
Therefore, the effect of the microenvironment should be considered when evaluating the proliferative capability of renal cancer cells in the experimental settings. (nih.gov)
tumor microenvironment. (nih.gov)
Findings suggest therapeutic strategies should be aimed at interfering with cancer cell reprogramming of microenvironment cues ( Nature ). (frontlinegenomics.com)
Our findings identify an lncRNA that is induced by cues from the tumor microenvironment and that interacts with PTBP1 to regulate alternative splicing, potentially providing a therapeutic target and predictive biomarker for metastatic CRC. (bvsalud.org)
We hypothesize that tumor-derived exosomes endow the fibroblasts in tumor microenvironment with invasive phenotype to the benefit of tumor metastasis. (dovepress.com)
2 , 3 Tumor cell-derived exosomes carry particular molecular components originated from donor cells to mediate tumorigenesis and drive tumor progression by regulating tumor microenvironment. (dovepress.com)

Cancer stem cells (CSCs) play an important role during metastasis, but the dynamic behavior and induction mechanisms of CSCs are not well understood. (nature.com)
Interestingly, while KLF6-SV1 overexpression increased metastasis, it did not affect localized tumor growth. (jci.org)
Mitochondrial DNA (mtDNA) carrying certain pathogenic mutations or single nucleotide variants (SNVs) enhances the invasion and metastasis of tumor cells, and some of these mutations are homoplasmic in tumor cells and even in tumor tissues. (biomedcentral.com)
It remains unclear whether metastasis-enhancing pathogenic mutant mtDNA in tumor cells is intercellularly transferred between tumor cells and stromal cells. (biomedcentral.com)
We and others previously showed that certain mitochondrial DNA (mtDNA) somatic mutations or single nucleotide variants (SNVs) enhanced the invasion and metastasis of mouse and human cell lines, e.g. (biomedcentral.com)
Furthermore, the frequency of predicted pathogenic mtDNA mutations was significantly correlated with distant metastasis in patients with non-small cell lung carcinoma (NSCLC) and colon cancers [ 2 ]. (biomedcentral.com)
The ECM a researcher chooses has a significant effect on cancer cell proliferation, initiation, invasion, and metastasis, and it alters how the tumor model responds to drug therapy. (corning.com)
Collagen is the most abundant fibrous protein found in mammals' ECMs, and it plays a critical role in tumor growth and metastasis because it promotes cell adhesion and migration. (corning.com)
Spheroids have also been used to study transcoelomic metastasis in ovarian cancer, in which primary tumor cells seep into the abdominal cavity, where they form spheroids and then travel through fluids to secondary sites. (corning.com)
This model however, attributes tumorigenesis, metastasis and chemoresistance to a distinct cell population with a stem-like phenotype that can be identified using selective markers. (tmc.edu)
In another set of mouse experiments that tracked the timeline of melanoma metastasis to the brain, the team found that individual melanoma cells lacking amyloid beta could spread to the brain and survive there for about a week. (cancer.gov)
We reported here that plakoglobin, as a key component in cell adhesion, can promote collective metastasis through facilitating IMPC clusters formation. (jcancer.org)
In comparing the clinicopathological features of 451 IMPC patients and 282 IDC-NST patients, our results showed that tumor emboli were significantly higher in IMPC patients and were associated with a high frequency of metastasis. (jcancer.org)
Leveraging the inverse relationship between cell pluripotency and miR-145 expression, we successfully separated highly tumorigenic, metastasis-prone (miR-145low) cells from poorly tumorigenic, nonmetastatic (miR-145high) counterparts. (visualsonics.com)
Gene expression and functional studies of the two cell populations identified the EPHB2 receptor as a prognostic biomarker in patients with EwS and a major promoter of metastasis. (visualsonics.com)
We hypothesised that if CSC are responsible for metastasis, then the SW620 cell line may be enriched in CSC compared to the SW480 cell line and therefore should be the more chemoresistant of the two cell lines. (petrocollapse.org)
Tumor growth and the metastasis are asset after 10 weeks. (jove.com)
It is confirmed that large variety of miRNAs in exosomes play important roles in tumor metastasis and invasion. (dovepress.com)
A novel instrument for detecting cancer cells in the blood could lead to individualized cancer treatments for patients suffering metastasis. (nfcr.org)
While CTCs occur once in a billion cells, and therefore are extremely rare, they nevertheless may hold a key to metastasis-the stage responsible for the vast majority of cancer deaths. (nfcr.org)
Angiogenesis is essential in the process of carcinogenesis to facilitate tumor progression and metastasis. (spandidos-publications.com)
Additionally, the expression and activity of matrix metalloproteinases (MMPs) are increased in almost every type of human cancer, which may be associated with cell invasion, metastasis and angiogenesis. (spandidos-publications.com)
Subcutaneous injection of cell lines induced tumor formation only in NSG mice and was accompanied by metastasis to the liver, adrenal glands, skull and bone marrow. (iiarjournals.org)
The main clinical problem in treating NB is metastasis, specifically metastatic stages III and IV according to the International Neuroblastoma Staging System ( 1 , 2 ), because metastatic lesions are often resistant to current therapies. (iiarjournals.org)
However, such an artificial basement membrane-like matrix is known to enhance tumor growth and metastasis ( 33 , 34 ), hence modifying the intrinsic tumorigenic properties of cancer cells and the pathogenesis of the related disease itself. (iiarjournals.org)
Objective To screen and establish a spontaneously high organotrophic cell subline of metastatic breast cancer and to validate characteristic and the organotropism of the lung metastasis cell subline obtained from the screening. (magtechjournal.com)
Methods By using the experimental lung metastasis, mouse breast cancer cells line 4T07 were injected into tail vein of mouse. (magtechjournal.com)
By using the spontaneous metastasis model, the mammary fat pad was inoculated with tumor cells. (magtechjournal.com)
Cell morphology was microscopied with HE staining and an in vivo imaging system was performed to detect the migration and lung-specific metastasis. (magtechjournal.com)
Flow cytometry was used to analyze the changes of immune cells in primary site, metastasis and spleen. (magtechjournal.com)
HE staining microscopy results verified tumor metastasis in the lung. (magtechjournal.com)
Conclusions Cell sublines with high organ-specific metastasis ability are obtained through combination of serial screening of experimental and spontaneous metastasis and in vitro culture expansion. (magtechjournal.com)
A perspective on cancer cell metastasis[J]. Science, 2011, 331: 1559-1564. (magtechjournal.com)
Using cell culture, animal and patient studies, we investigated the biological role of microRNA-96 (miR-96) and microRNA-379 (miR-379) in prostate cancer bone metastasis. (lu.se)
Downregulation of miR-379 enhanced incidence of bone metastasis in mice, increased colony formation potential in osteoblast-conditioned media, and increased cell growth. (lu.se)
Noninvasive prediction of axillary lymph node breast cancer metastasis using morphometric analysis of nodal tumor microvessels in a contrast-free ultrasound approach. (medscape.com)
It is therefore imperative to identify molecular targets and develop novel anti-metastatic therapies that will stop, reduce or delay the spread and growth of breast cancer metastasis. (lu.se)
Low Wnt-5a expression in a primary breast tumor is associated with shorter recurrence free survival, suggesting that Wnt-5a expression acts to restrict breast cancer metastasis. (lu.se)
We found that SOX9 is upregulated in SWCNT-exposed cells, which is consistent with their abilities to induce tumor formation and metastasis in vivo. (cdc.gov)
Furthermore, SOX9 knockdown suppressed tumor metastasis and the expression of the stem cell marker ALDH1A1. (cdc.gov)

Macrophages are emerging as major cellular factors in the tumor ecosystem that can influence the stem phenotype and cancer progression. (nature.com)
It is within the cell-autonomous context that ST6GAL1 involvement in cancer progression has been interpreted. (nature.com)
Cancer cell-extrinsic mechanisms, which are poorly understood, are also believed to contribute to disease progression and the heterogeneous genetic mutations with diverse presentations. (nature.com)
Intratumoral heterogeneity, including in clear cell renal cell carcinoma, is a potential cause of drug resistance and metastatic cancer progression. (nih.gov)
We specified the heterogeneous population marked by endoglin (also known as CD105) in a preclinical model of clear cell renal cell carcinoma progression. (nih.gov)
The reasons for this are multiple and include the variable and often long times to progression, the fact that direct measurements on target tumors are often not possible, and also include patient- intrinsic effects related to both patient and tumor heterogeneity. (biomedcentral.com)
Results Comparison of biological characteristics of the paired SW480 and SW620 cancer cell lines The morphology of SW480 and SW620 cells were analysed by phase contrast microscopy to reveal two cell morphologies (Fig. model of cancer progression. (petrocollapse.org)
As the SW480 and SW620 cell lines developed from the same genetic background, Splitomicin they provide an model to study the cellular changes that occur during cancer progression and development of a metastatic phenotype. (petrocollapse.org)
My research interests are focusing on the activity of tyrosine kinases, targeted inhibitors, effects of ascitic fluid ,and anti-tumour properties of natural food compounds in the progression of advanced ovarian cancer. (otago.ac.nz)
Tumor-derived exosomes are messengers that participate in tumor progression. (dovepress.com)
Our results suggest that tumor-derived exosomes may facilitate stromal fibroblasts an aggressive phenotype to equip the tumor progression. (dovepress.com)
Autophagic Degradation of NBR1 Restricts Metastatic Outgrowth during Mammary Tumor Progression. (ucsf.edu)
Proteomic analysis of an independent cohort of more than 390 patient specimens further documented the relationship between increased α-tubulin acetylation and the aggressive behaviors of basal-like breast cancers, with a trend toward increased risk of disease progression and death in patients with high-intensity α-tubulin acetylation in primary tumors. (nih.gov)
Another important mechanism for the progression of colorectal cancer is the ability of the transformed cells to invade and traverse the basement membrane, which in turn facilitates the conversion of adenomas into carcinomas and then metastases ( 1 ). (spandidos-publications.com)

Collagen type I hydrogels have been used to grow MCTS from osteosarcomas, breast cancer cells, human colorectal cancer cells, prostate cancer cell lines, and primary cancer cells from colorectal cancer patients, according to the Science Advances study. (corning.com)
Here, we discovered that the long noncoding RNA (lncRNA) MIR99AHG regulated alternative splicing to alter the activity of a chromatin remodeler and promote metastatic behaviors in colorectal cancer (CRC). (bvsalud.org)
Combination of IL-2/15/21 can enhance TIL expansion in lung and colorectal cancer and promote CD8+ T cell percentage as well as TCR clone diversity compared to IL-2 alone [ 13 ]. (biomedcentral.com)
Scientists at The Institute of Cancer Research and the Royal Marsden NHS Foundation Trust in the U.K. have found that colorectal cancer (CRC) tumors can switch off expression of a cell surface molecule that is a key target of immunotherapy, which stops the treatment from working. (genengnews.com)
Our investigations are ongoing in biomarkers of resistance and potential new targets for therapeutic intervention in colorectal cancer, melanoma and Merkel cell carcinoma. (massgeneral.org)

Extracellular ST6GAL1, present in cancer exosomes or the freely soluble recombinant sialyltransferase, compensates for insufficient intrinsic ST6GAL1 by boosting cancer cell proliferation and increasing invasiveness. (nature.com)
Our data indicate that extracellular ST6GAL1 from remote sources can compensate for cellular ST6GAL1-mediated aggressive tumor cell proliferation and invasive behavior and has great clinical potential for extracellular ST6GAL1 as these molecules are in the extracellular space should be easily accessible targets. (nature.com)
These specially tuned fields have no effect on quiescent cells while having an anti-proliferation and destructive effect on mitotic cells. (biomedcentral.com)
Results suggest leukocyte and T-cell proliferation have improved predictability performance over tumour mutational burden ( Nature ). (frontlinegenomics.com)
The hyperactive receptors drove tumor cell proliferation even in the absence of estrogen. (ludwigcancerresearch.org)
In fact, in addition to their potent vasodilating effect, EETs have potent anti-inflammatory properties, inhibit platelet aggregation, promote fibrinolysis, and reduce vascular smooth muscle cell proliferation. (frontiersin.org)
Originally isolated from the HPC-Y5 pancreatic cell line, MPF has been suggested to play a role in the proliferation and differentiation of megakaryoctyes, and the regulation of resultant platelet production. (peprotech.com)
Among its different constituents, Astragalus polysaccharides have been most widely studied particularly for their immunopotentiating properties, such as stimulation of murine B cell proliferation and cytokine production ( 6 ). (spandidos-publications.com)
Our results indicated that AST inhibits cell proliferation through phase-specific cell cycle arrest and caspase-dependent apoptosis. (spandidos-publications.com)
In addition, tumor growth rates and organ infiltration patterns associated with injected NB cell lines correlated with the in vitro proliferation properties and genetic markers of poor prognosis in NB patients. (iiarjournals.org)
Protein transport between the nuclear and the cytoplasmic compartment is a key factor for cell viability and proliferation. (massgeneral.org)
In: Chemically induced cell proliferation: Implications for risk assessment. (cdc.gov)

We found that shRNA knockdown of intrinsic ST6GAL1 expression resulted in decreased ST6GAL1 cargo in the exosome-like vesicles as well as decreased breast tumor cell growth and invasive behavior in 3D in vitro cultures. (nature.com)
This receptor is induced and required to survive stressors, like anchorage independence, serum starvation and chemotherapy treatment, which are similar in vitro conditions used to enrich for cells with tumor initiating potential. (tmc.edu)
Both in vitro and in vivo data showed overexpression of plakoglobin in both the cell membrane and the cytoplasm of IMPC clusters. (jcancer.org)
Tumor-infiltrating lymphocyte (TIL) therapy is a type of adoptive cellular therapy by harvesting infiltrated lymphocytes from tumors, culturing and amplifying them in vitro and then infusing back to treat patients. (biomedcentral.com)
To this end, Dr. Rosenberg and others developed a "Young TIL" approach that rapidly expands TIL for administration without in vitro selection for tumor reactivity [ 8 ], which markedly improves the timeliness of TIL production as well as its survival and efficacy in vivo (Fig. 1 ). (biomedcentral.com)
Currently, the most widely-used TIL production method is to isolate infiltrating lymphocytes from tumor tissues and then culture and expand these cells in vitro. (biomedcentral.com)
Data from in vitro translation experiments and transfection of melanoma cells with bicistronic vectors documented that MELOE-3 is exclusively translated by the classical cap-dependent pathway. (oncotarget.com)
Furthermore, in vitro stimulation of PBMC from 6 healthy donors with overlapping peptides from MELOE-1 or MELOE-3 revealed a very scarce MELOE-3 specific T cell repertoire as compared to the abundant repertoire observed against MELOE-1. (oncotarget.com)
Resveratrol shows anti-tumour activities in an in vitro in various tumour types including ovarian cancer. (otago.ac.nz)
The "Biomimetic Systems for Cell Engineering" group is a multidisciplinary research group focussing its research activities on the development and application of new artificial systems that mimic tissue micro and nanofeatures for biomimetic in vitro assays. (ibecbarcelona.eu)
Along with this line, we are working towards the following objectives: (i) to engineer and validate a complex in vitro model of small intestinal epithelium, (ii) to validate a novel in vitro model of engineered cardiac tissue and (iii) to engineer a novel vessel-on-chip to reproduce the tumor metastatic environment. (ibecbarcelona.eu)
Wound healing and Transwell assays were performed to detect migration of cell lines in vitro . (magtechjournal.com)
There is an unmet clinical need to extract living circulating tumor cells (CTCs) for functional studies and in vitro expansion to enable drug testing and predict responses to therapy in metastatic cancer. (lu.se)
The novel A2 method provides extensive elimination of WBCs combined with the gentle recovery of viable cancer cells suitable for downstream functional analyses and in vitro culture. (lu.se)
Indeed, SOX9 knockdown inhibited anchorage-independent cell growth in vitro and lung colonization in vivo in a mouse xenograft model. (cdc.gov)

This bypasses the need to systemically administer IL-2 as well as the challenges of delivering the drug itself into solid tumors," Saltzman explained. (aacr.org)
Looking forward, the researchers plan to evaluate the therapy in a randomized phase III clinical trial and are also investigating the potential of this approach to treat other solid tumors. (aacr.org)
In mice, the modified E. coli selectively colonized solid tumors and attracted a therapeutic radioisotope that had been delivered systemically. (aacr.org)
Its diverse TCR clonality, superior tumor-homing ability, and low off-target toxicity endow TIL therapy unique advantages in treating solid tumors compared with other adoptive cellular therapies. (biomedcentral.com)

In an effort to explain tumoral heterogeneity, the tumor initiating cell model (TIC) states that tumors are composed of cells that form the majority of the tumor and are terminally differentiated. (tmc.edu)
Single cell RNA sequencing of endothelial cell heterogeneity in breast cancer identifies lipid-processing subtype that could offer a potential route for future treatment regimes ( Nature ). (frontlinegenomics.com)
However, the metastable nature of tumor heterogeneity, which allows cells to transition between strong and weak tumorigenic phenotypes, and the lack of reliable markers of tumor-promoting properties hamper identification of the most relevant cells. (visualsonics.com)

When administered to mice alone or in combination with immunotherapy, the bacteria activated tumor-specific immune cells that infiltrated and suppressed the growth of primary and metastatic melanoma lesions. (aacr.org)
Melanoma cells (green and blue) in the brain, being enveloped by astrocytes (orange branches). (cancer.gov)
The researchers found that melanoma cells that travel to the brain produce their own supply of amyloid beta and that this protein is necessary for their survival. (cancer.gov)
Treating the mice with drugs that block the protein greatly reduced melanoma cells' ability to survive in the brain. (cancer.gov)
The results, including from tests in mice, seemed to confirm that the melanoma cells could spread to other organs without the protein but that it was needed for the cells to establish tumors in the brain. (cancer.gov)
In mouse brains and in cultured cells taken from rat brains, Dr. Hernando-Monge and her team found that the amyloid beta produced by melanoma cells interacts directly with a type of brain cell called an astrocyte . (cancer.gov)
Single-cell and spatial transcriptomics reveals the hierarchical nature of tumour growth in metastatic melanoma. (frontlinegenomics.com)
The earliest attempt of TIL therapy in the clinic can be traced back to 1988 and achieved a 60% objective response rate (ORR) in metastatic melanoma [ 1 ]. (biomedcentral.com)
MELOE-1 and MELOE-2, two highly specific melanoma antigens involved in T cell immunosurveillance are produced by IRES-dependent translation of the long « non coding » and polycistronic RNA, meloe . (oncotarget.com)
Using a sensitive tandem mass spectrometry technique, we detected the presence of MELOE-3 in total lysates of both melanoma cells and normal melanocytes. (oncotarget.com)
In conclusion, within the MELOE family, IRES-dependent antigens represent the best T cell targets for immunotherapy of melanoma. (oncotarget.com)
In this respect, we have previously identified two melanoma antigens, namely MELOE-1 and MELOE-2, that were recognized by tumor-infiltrating lymphocytes (TIL) from HLA-A0201+ patients who remained relapse-free following TIL transfer in an adjuvant setting [ 3 ]. (oncotarget.com)
After ingestion of melanoma-derived exosomes during incubation, mouse embryonic fibroblasts cells emerged cellular invasiveness with the presentation of a marked increase in miR-21 expression. (dovepress.com)
MiR-21 promoted invasion of fibroblasts by down-regulation of tissue inhibitor of metalloproteinase 3 (TIMP3) expression and increasing of matrix metalloprotein (MMP) expression in fibroblast cells via melanoma-derived exosomes in a time-dependent manner. (dovepress.com)
8 , 9 The high-invasion and metastatic tendency of melanoma tumors complicates treatment of the malignancy. (dovepress.com)
Studies have shown that miR-21 can promote invasion of melanoma cells. (dovepress.com)
10 High expression of miR-21 in melanoma cells inhibits mRNA expression of crucial tumor suppressor proteins such as the tissue inhibitor of metalloproteinases 3 (TIMP3). (dovepress.com)

The main purpose of this study is to gather information about the investigational drug pexidartinib, which may help to treat tumors of pigmented villonodular synovitis (PVNS) or giant cell tumor of the tendon sheath (GCT-TS). (stanford.edu)
TIL therapy is a type of adoptive cellular therapy leveraging the patient's own immune system to treat tumors. (biomedcentral.com)
Genetic testing on captured CTCs may give doctors a way to more effectively treat tumors or stop them from spreading. (nfcr.org)

Mortality is attributable largely to metastatic disease and a dearth of effective therapeutic interventions. (biologists.com)
Data from recent clinical trials have highlighted the potential for this therapeutic modality to effect potent anti-tumor activity. (biomedcentral.com)
Metastatic cases of breast cancer pose the primary challenge in clinical management of this disease, demanding the identification of effective therapeutic strategies that remain wanting. (nih.gov)
Another report described the intravenous ( i.v. ) injection of NB cell lines into NSG, SCID-beige, and nude mice, and demonstrated that the use of NSG mice was advantageous in therapeutic and survival studies ( 35 ). (iiarjournals.org)
However, the metastatic potential of free NB cells injected into NSG mice needs to be investigated further in order to validate the use of these mice as a relevant xenograft model of human NB and as a preclinical model for the development of new therapeutic strategies. (iiarjournals.org)
Metastatic hetero-geneity of breast cancer: molecular mechanism and potential therapeutic targets[J]. Semin Cancer Biol, 2020, 60: 14-27. (magtechjournal.com)

When MitoTracker Deep Red-labeled high-metastatic Lewis lung carcinoma A11 cells carrying the ND6 G13997A mtDNA mutation were cocultured with CellLight mitochondria-GFP-labeled low-metastatic P29 cells harboring wild-type mtDNA, bidirectional transfer of red- and green-colored vesicles, probably mitochondria-related EVs, was observed in a time-dependent manner. (biomedcentral.com)
Further, lung fibroblasts were cultured with and without cell supernatants from previously established cell cultures for 24 h. (medscimonit.com)
The exposure of lung fibroblasts to supernatant from mesothelial cell (with or without adenocarcinoma) did not affect collagen secretion. (medscimonit.com)
RR had an anti-tumour and anti-metastatic effect in the Lewis lung cancer model in mice (potentiated cyclophosphamide). (doctorschar.com)
Although Mesothelin is expressed at restricted levels by normal mesothelial cells of the pleural,pericardial, and peritoneal membranes, aberrant expression has been documented in the aforementioned cancers, as well as in endometriod uterine adenocarcinomas and squamous cell carcinomas of the esophagus, stomach, lung, and cervix. (peprotech.com)
Results A mouse breast cancer lung-specific metastatic cell subline (4T07LuM) was successfully established. (magtechjournal.com)
in vivo imaging results validated that the fluorescent signal was mainly concentrated in the lung after injection of 4T07LuM cells labeled with luciferase in situ . (magtechjournal.com)
Establishment and validation of lung-specific metastatic cell subline of mouse breast cancer[J]. Basic & Clinical Medicine, 2022, 42(7): 1083-1091. (magtechjournal.com)
These 2 cases of community-acquired K. pneumoniae serotype K1 and K2 liver abscesses with metastatic spread to the lung and urinary systems in previously healthy white patients from Germany differ from previously published cases. (cdc.gov)
Breast metastatic tumors in lung can be substituted by lung-derived malignant cells transformed by alternative splicing H19 lncRNA. (medscape.com)
Simple Summary: This study identified a set of 73 microRNAs (miRNAs) that can accurately detect lung cancer tumors from normal lung tissues. (cdc.gov)
Based on the consistent expression patterns associated with patient survival outcomes and in tumors vs. normal lung tissues, 10 miRNAs were considered to be putatively tumor suppressive and 4 miRNAs were deemed as oncogenic in lung cancer. (cdc.gov)
The majority of lung cancer patients are diagnosed with metastatic disease. (cdc.gov)
This study identified a set of 73 microRNAs (miRNAs) that classified lung cancer tumors from normal lung tissues with an overall accuracy of 96.3% in the training patient cohort (n = 109) and 91.7% in unsupervised classification and 92.3% in supervised classification in the validation set (n = 375). (cdc.gov)
In this study, we linked SOX9, a stem cell associated transcription factor, to the neoplastic-like properties of human lung epithelial cells chronically exposed to a low-dose of single-walled carbon nanotube s (SWCNTs). (cdc.gov)

Highly malignant derivatives of human clear cell renal cell carcinoma OS-RC-2 cells were established as OS5Ks by serial orthotopic inoculation in our previous study. (nih.gov)
Malignant cells might have an impact on the mesothelial response to applied sclerosing agents and, in consequence, on the efficacy of pleurodesis. (medscimonit.com)
7 It has been shown that miR-21 is a promoter of oncogenes and is highly expressed in several malignant tumors. (dovepress.com)
[ 12 ] The bone is destroyed as the tumor advances, suggesting a malignant process. (medscape.com)

The study's results confirmed previous findings that a treatment protocol of cisplatin and radiation was superior to radiation alone and confirmed the feasibility of using primary tumor cells for spheroid formation, which could yield MCTS that more closely mimic in vivo tumor behavior. (corning.com)
Our study provides a simple and powerful means to identify and isolate tumor cells that display aggressive behavior. (visualsonics.com)
In recent years, a few studies have started to investigate the biological activity of ascitic fluid,which is believed to influence behavior of ovarian cancer cells. (otago.ac.nz)
Taken together, our results identify a tight correlation between acetylated α-tubulin levels and aggressive metastatic behavior in breast cancer, with potential implications for the definition of a simple prognostic biomarker in patients with breast cancer. (nih.gov)

We also utilize cell culture techniques to maintain multiple tumor cell lines (metastatic and nonmetastatic) that are used in rat and mouse tumor-bearing models. (unco.edu)

60% of circulating tumor cells. (nature.com)
The presence of high expressing epithelial cell adhesion molecule (EpCAM high ) circulating tumor cells (CTC) enumerated by CellSearch ® in blood of cancer patients is strongly associated with poor prognosis. (oncotarget.com)
Tumor growth and circulating tumor cells are asset after 10 days of implantation before the embryo hatches. (jove.com)
Developed by a Massachusetts General Hospital team led by Daniel Haber, M.D., Ph.D. , a renowned cancer scientists supported by the National Foundation for Cancer Research since 2003, the business card-sized CTC-iChip captures circulating tumor cells-referenced in the name of the device- "simply" by running blood from a cancer patient across its surface. (nfcr.org)
Circulating tumor cells are tumor cells that have become detached from the primary tumor and enter blood circulation. (nfcr.org)
Additionally, the CTC-iChip captures circulating tumor cells when they are still viable-dead cells are not useful for long-term analysis. (nfcr.org)

Here we demonstrated that increased expression of a splice variant of the Kruppel-like factor 6 (KLF6) tumor suppressor gene, known as KLF6-SV1, in tumors from men after prostatectomy predicted markedly poorer survival and disease recurrence profiles. (jci.org)
Cancer stem cells (CSCs) are thought to be a source of tumor recurrence due to their stem cell-like properties. (biomedcentral.com)
The lingering mysteries of metastatic recurrence in breast cancer[J]. Br J Cancer, 2021, 124: 13-26. (magtechjournal.com)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
The tumor size and the number of proliferating cells were reduced in the tumor tissues obtained from the formononetin-treated group. (spandidos-publications.com)

In the phase II clinical trial, presented by Daniel Saltzman, MD, PhD , a professor and oncologist at the University of Minnesota Medical School, 28 patients with metastatic pancreatic cancer received an oral dose of modified Salmonella in combination with a standard chemotherapy regimen. (aacr.org)

KLF6-SV1 inhibition using RNAi induced spontaneous apoptosis in cultured PCa cell lines and suppressed tumor growth in mice. (jci.org)
Our data indicate that plakoglobin promotes tumor cluster formation in IMPC and downregulates apoptosis in the cell clusters through activation of PI3K/Akt/Bcl-2 signaling. (jcancer.org)

Nevertheless, the successful application of TIL therapy currently is still limited to several types of tumors. (biomedcentral.com)

In 2 complementary mouse models of metastatic PCa, KLF6-SV1-overexpressing PCa cells were shown by in vivo and ex vivo bioluminescent imaging to metastasize more rapidly and to disseminate to lymph nodes, bone, and brain more often. (jci.org)
Hydrogel-based ECMs offer a variety of properties that allow researchers to scaffold MCTS and improve their similarity to in vivo tumors. (corning.com)
The concept of enhancing cellular immunity through the transfer of ex-vivo expanded T cells was pioneered by Greenberg et al. (biomedcentral.com)
Using mouse models, we validated the metastatic potential of tumor clusters was higher than single cells in vivo . (jcancer.org)
In addition, in vivo serial transplantation assays, dye exclusion assays, and isolation via cell surface specific antigen profile methods are now used to identify CSCs. (biomedcentral.com)

A highly metastatic cell subline stably expressing luciferase was obtained by transfection and screening. (magtechjournal.com)

In syngeneic mouse subcutaneous tumors formed by a mixture of A11 and P29 cells, the mitochondria-related EVs released from A11 cells reached distantly positioned P29 cells and CAFs. (biomedcentral.com)
Recently, NSG mice have been used in an NB xenograft model for studying tumor-infiltrating human lymphocytes in subcutaneous ( s.c. ) tumors of NB cells embedded in Matrigel® ( 32 ). (iiarjournals.org)
SCR-6852, an oral and highly brain-penetrating estrogen receptor degrader (SERD), effectively shrinks tumors both in intracranial and subcutaneous ER + breast cancer models. (medscape.com)

Here, we employ high-resolution intravital microscopy using a CSC biosensor to directly observe CSCs in live mice with mammary tumors. (nature.com)
CSCs display the slow-migratory, invadopod-rich phenotype that is the hallmark of disseminating tumor cells. (nature.com)
At the apex of the hierarchy are the cancer stem cells (CSCs). (nature.com)
CSCs have an enhanced ability to self-renew, which makes them uniquely capable of initiating and sustaining primary and metastatic tumor growth 3 . (nature.com)
MicroRNAs (miRNAs) regulate both normal stem cells and CSCs, and dysregulation of miRNAs has an important role in tumorigenesis. (biomedcentral.com)
Our results indicate that dysregulation of miRNA may play a role in the stem cell-like properties of ovarian CSCs. (biomedcentral.com)
Using this method, ovarian CSCs from patients with ascites are first isolated and their ability to exhibit stem cell-like properties is examined [ 6 ]. (biomedcentral.com)
In this study, miRNA expression profiles of a CD133 + spheroid-forming subpopulation of OVCAR3 ovarian cancer cells were investigated to identify miRNA expression profiles that contribute toward the characteristics of CSCs in ovarian cancer. (biomedcentral.com)
The SP assay identifies putative CSCs based on the high activity of the ATP-binding cassette transporter protein (ABC)G2, which is also implicated in drug resistance due to its role in the efflux of chemotherapeutics from the cell (16,17). (petrocollapse.org)
In particular, whether or not the presence of CSCs determines the metastatic potential of the tumour has yet to be fully elucidated (3). (petrocollapse.org)
SOX9 depletion also suppressed the formation of cancer stem-like cells (CSCs), as determined by tumor sphere formation and aldehyde dehydrogenase (ALDH) activity (Aldefluor) assays. (cdc.gov)

RR reduced the toxicity and liver damage associated with the anti-tumour drug Adriamycin without reducing efficacy of the anti-tumour drug. (doctorschar.com)
Therefore, anti-tumour properties of natural compounds are intensively investigated. (otago.ac.nz)

Targeting of the most aggressive tumor cell subpopulations is key for effective management of most solid malignancies. (visualsonics.com)

In mammary gland development, Notch-dependent heterotypic signaling between resident tissue macrophages and mammary stem cells supports survival and function of the normal mammary stem cell 20 . (nature.com)
Treatments by TTFields/drug combinations resulted in a similar increased reduction in cell survival of wild type and MDR cells. (biomedcentral.com)
Therapy using checkpoint inhibitor immunotherapies can increase survival in some CRC patients with hypermutated microsatellite instability (MSI) tumors, but are ineffective against the majority of cases, including microsatellite stable (MSS) CRC. (genengnews.com)

This review will focus on biomarker studies as they relate to T cell therapy trials, and more specifically: i. (biomedcentral.com)

Metastatic prostate cancer (PCa) is one of the leading causes of death from cancer in men. (jci.org)
E ) Left: DNA microarray analysis of PCa demonstrated downregulation of KLF6 mRNA in hormone-refractory metastatic PCa (HR-MET) compared with both noncancerous prostate tissue and localized PCa. (jci.org)
In Paper IV, we compared the biological functions of unedited and edited miR-379 in prostate cancer cells. (lu.se)

Therefore, the recently described NOD/SCID/interleukin-2 receptor γ ( Il2rg ) null (NSG) mice have been shown to be one of the most immunodeficient mouse hosts to date since these mice are deficient in both T and B lymphocytes, do not have any NK cells, and display impaired dendritic cell functions ( 22 ). (iiarjournals.org)

demonstrating their involvement in heterotypic cell adhesion and the metastatic spread of cancer. (peprotech.com)
We also showed that miR-96-transfected cells had increased cell-cell adhesion to both each other and osteoblasts, and an increased colony formation potential. (lu.se)
Moreover, Wnt-5a is known to inhibit migration of breast epithelial cells in culture and expression of Wnt-5a potentiates activation of the receptor tyrosine kinase DDR1, a collagen receptor implicated in cell adhesion and migration. (lu.se)
Finally, we found that phospho-DARPP-32 inhibited the activity of the focal adhesion kinase (FAK) in MCF-7 breast cancer cells, and that MCF-7 cells expressing phospho-DARPP-32 displayed less filopodia formation. (lu.se)

Our previous findings demonstrated that formononetin initiates growth-inhibitory and pro-apoptotic activities in human colon cancer cells. (spandidos-publications.com)
These findings suggest that formononetin inhibits angiogenesis and tumor cell invasion, and thus support its use in the treatment of advanced and metastatic colon cancers. (spandidos-publications.com)

Even though the exact insight mechanisms of how ascitic fluid exert to ovarian cancer cells is poorly defined, my research suggests that ascitic fluid can increase expression of oncogenic proteins in ovarian cancer cells and reduce the cellular uptake of targeted drugs. (otago.ac.nz)
These tumors have a similar cellular physiology, as well as a shared chromosomal translocation. (medscape.com)

Furthermore, macrophages were shown to contribute to a niche that can support and maintain a breast CSC (BCSC) phenotype through heterotypic CD90/EphA4 signaling between macrophage and tumor cell 14 . (nature.com)

Mice underwent intrarenal implantation using luciferase-expressing Renca, a murine renal adenocarcinoma cell line. (biologists.com)
Proteolytic cleavage of Mesothelin yields a soluble, polypeptide fragment designated megakaryocyte potentiating factor (MPF) based on its ability to stimulate megakaryocyte colony-forming activity of murine interleukin-3 in murine bone marrow cell cultures. (peprotech.com)
Improved handling and embedding schemes for cultured murine neuroretinal explants. (ucsf.edu)

Therefore, we determined the miRNA expression profile of a CD133 + spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line. (biomedcentral.com)
To understand these factors, I am using three dimensional (3D) cell cultures, chicken embryos and a syngeneic mouse model to investigate the biological activity in ovarian cancer. (otago.ac.nz)
Receptor tyrosine kinases including EGFR, HER-2 and c-Met and non receptor tyrosine kinase including Jaks are selectively expressed in primary tissue and metastatic tumour of advanced ovarian cancer cells. (otago.ac.nz)
Despite wealth information of these tyrosine kinase expressions and activation, little is known about the biological activity of tyrosine kinases in ovarian cancer cells are present in ascitic fluid. (otago.ac.nz)
This project is investigating the expression and activation of tyrosine kinases in ovarian cancer cells isolated from ascitic fluid. (otago.ac.nz)
In addition, the ascitic fluid contains various of cell types including white blood cells, mesenchymal cells, red blood cells and ovarian cancer cells (single cells and clusters). (otago.ac.nz)

A study published in Anticancer Research on head and neck cancers charted the development of a method for generating MCTS from tumor biopsies, the first step toward creating a method of assessing individual susceptibility to chemotherapy drugs and radiation therapy. (corning.com)
We also showed that cisplatin chemotherapy was able to inhibit tumor growth. (iiarjournals.org)
Correction: Changes in the tumor oxygenation but not in the tumor volume and tumor vascularization reflect early response of breast cancer to neoadjuvant chemotherapy. (medscape.com)
as monotherapy for the treatment of those patients who have received at least two chemotherapy regimens for their metastatic disease. (who.int)
Biocon Biologics Ltd), BT-ON014 - in combination with docetaxel for the treatment of those patients who have not received chemotherapy for their metastatic disease - in combination with an aromatase inhibitor for the treatment of postmenopausal patients with hormone-receptor positive MBC, not previously treated with trastuzumab. (who.int)

Although endoglin has been proposed as a marker for renal cancer-initiating cells, the OS5K-3 ENG + population did not enrich other reported cancer-initiating cell markers or differentiate into the ENG - population. (nih.gov)

In collaboration with Mayo Clinic clinicians and other researchers, rare PGL tumors are being grown in culture and in immunodeficient mice. (mayo.edu)
12) Shown to act as antimutagenic drug in bone marrow of cells of mice due to its ability to raise the efficiency of the intracellular DNA repair mechanism. (doctorschar.com)
The reduction in tumor volume as well as pro-apoptotic and anti-proliferative effects in HT-29 nude mice xenograft are comparable to those produced by 5-FU. (spandidos-publications.com)
In order to develop a relevant xenogenic animal model of neuroblastoma (NB), we compared the tumorigenicity and metastatic potential of SK-N-SH and SK-N-DZ NB cell lines in nude mice and NOD/SCID Il2rg null (NSG) mice. (iiarjournals.org)
These results clearly demonstrate the higher tumorigenic and metastatic potential of NB cells in NSG mice. (iiarjournals.org)
Immunodeficient mice that cannot reject xenotransplanted cells have been shown to be the best living recipient for developing xenograft models of human cancer ( 6 - 8 ). (iiarjournals.org)
To date, the most extensively used mouse strain for developing NB xenograft models are SCID mice ( 9 - 12 ), SCID/beige mice ( 13 , 14 ) and nude mice ( 5 , 15 - 19 ) that are all T-cell deficient hosts. (iiarjournals.org)
This improved immunodeficient profile has established NSG mice as the new 'gold standard' for human haematopoietic stem cell ( 23 - 25 ) and cancer research. (iiarjournals.org)

Metastatic tumors expressed significantly less wild-type KLF6 mRNA compared with localized tumors. (jci.org)
We found 37 differentially expressed miRNAs in the CD133 + spheroid-forming subpopulation of OVCAR3 cells, 34 of which were significantly up-regulated, including miR-205, miR-146a, miR-200a, miR-200b , and miR-3, and 3 of which were significantly down-regulated, including miR-1202 and miR-1181 . (biomedcentral.com)
SW480 and SW620 cells displayed similar CSC profiles, however, SW480 cells demosntrated significantly greater tumoursphere forming efficiency over SW620 cells. (petrocollapse.org)
In addition, according to the results of Transwell and wound healing assays, 4T07LuM, compared to the parental cells 4T07, had shown a significantly more invasive migration( P ＜0.001). (magtechjournal.com)
Finally, according to the results of flow cytometry detection, 4T07LuM cell had showed significantly altered the immune cell ratios as compared to that of 4T07 cell. (magtechjournal.com)

They have determined that cells can switch between different types of motility namely rotation, random and amoeboid when placed in 3D biomimetic platforms. (wikipedia.org)
What is Cell Motility? (corning.com)
MIR99AHG was abundant in invasive CRC cells and metastatic tumors from patients and promoted motility and invasion in cultured CRC cells. (bvsalud.org)

In breast cancers from patients, the density of TMEM doorways correlates with the proportion of cancer cells expressing stem cell markers, indicating that in human breast cancer TMEM doorways are not only cancer cell intravasation portals but also CSC programming sites. (nature.com)
The sialyltransferase ST6GAL1 that adds α2-6 linked sialic acids to N-glycans of cell surface and secreted glycoproteins is prominently associated with many human cancers. (nature.com)
Our study has found that bowel cancers have a way of dodging even the newest of immunotherapies-changing their spots by altering the levels of a key molecule on the surface of cells, so that they become harder to recognize. (genengnews.com)
The device could dramatically improve treatment and diagnosis for many different types of metastatic cancers, including metastatic breast cancer and glioblastoma. (nfcr.org)

The invasion analysis showed that exosome-treated fibroblast cells had greater invasive capability as compared to untreated fibroblast cells, with the higher expressions of MMP2 and MMP9. (dovepress.com)
Transcriptomic profiling of T-cell populations in non-muscle invasive and muscle invasive bladder cancer. (lu.se)

Tumor-native ST6GAL1 promotes tumor cell behaviors such as invasion and resistance to cell stress and chemo- and radio-treatments. (nature.com)

She is currently a senior principal investigator in the laboratory of cell biology serving as head of the tissue morphodynamics section. (wikipedia.org)
The goal of this project is to evaluate the safety and preliminary efficacy of ExAblate magnetic resonance-guided high-intensity focused ultrasound (MRgHIFU) surgery in the treatment of soft tissue tumors of the extremities. (stanford.edu)
Improved superficial bladder cancer by improving characteristics of the urothelial tissue integration, parameters of leukocyte integrins, and T-cell immunity. (doctorschar.com)
The caption is: Mimicking small intestinal tissue by culturing intestinal-organoid derived cells on poly(ethylene glycol) diacrylate (PEGDA) microstructures. (ibecbarcelona.eu)

The purpose of this study is to investigate cell growth inhibition of wild type and the corresponding MDR cells by Tumor Treating Fields - TTFields, a new cancer treatment modality that is free of systemic toxicity. (biomedcentral.com)
Expression of both ENG (encoding endoglin) mRNA and protein were heterogeneously upregulated in OS5Ks, and the endoglin-positive (ENG + ) population exhibited growth dependency on endoglin in anchorage-independent cultures. (nih.gov)
Tamoxifen binds the estrogen receptor, and in so doing blocks its activity, choking off the ability of estrogen to fuel tumor growth. (ludwigcancerresearch.org)
It was determined that the SW480 and SW620 cells exhibited similar growth rates, although the SW480 cells were more migratory in wound healing assays on collagen and fibronectin matrices. (petrocollapse.org)
Because the HER2 gene expression oscillates between "on" and "off" spontaneously in these tumors, researchers reason that the two separate signaling pathways may need to be simultaneously turned off to halt either cell group from repopulating one another and beginning new abnormal growth. (nfcr.org)
The vascular endothelial growth factor (VEGF) is a well characterized angiogenic factor known to stimulate angiogenesis within a tumor ( 1 ). (spandidos-publications.com)
Subsequent study using multiple human cancer cell lines, including those of the colon, stomach and liver, further indicated the universal growth-inhibitory and pro-apoptotic effects of AST. (spandidos-publications.com)
We found that unedited miR-379 increased cell growth in androgen-independent cell lines, but inhibited cell growth in androgen-sensitive cell lines. (lu.se)

and why only neuroendocrine cells are at risk for tumorigenesis. (mayo.edu)
Many data have demonstrated that tumor exosomes are emerging mediators of tumorigenesis. (dovepress.com)

Thus MCT models are particularly useful, according to Science Advances, for developing therapies that target metastatic cells. (corning.com)
However, a significant number of patients are non responders to these therapies and thus, there is still room for improvement using antigen-specific immunotherapy, whether through vaccination or through T cell transfer. (oncotarget.com)

Because the TCA cycle is crucial for generating ATP, mutations that break the TCA cycle drastically reduce the ability of a cell to generate useful energy from blood nutrients. (mayo.edu)
Nonetheless, SDH mutations inherited from a parent set the stage for random and accidental loss of the remaining healthy SDH gene copy in somatic cells. (mayo.edu)
He and his colleagues found that about one-quarter of the tumors contained mutations that made the receptor hyperactive. (ludwigcancerresearch.org)
In cell culture experiments, the researchers found that it took extremely high doses of tamoxifen-doses too toxic for patients-to shut down metastatic tumors containing the mutations. (ludwigcancerresearch.org)

Ewing sarcoma tumors include Ewing sarcoma, Askin tumor, and peripheral primitive neuroectodermal tumors. (medscape.com)
In the early 1980s, Ewing sarcoma and the peripheral primitive neuroectodermal tumor were found to contain the same reciprocal translocation between chromosomes 11 and 22, t(11;22). (medscape.com)
The only noteworthy laboratory value documented in some but not all patients with melanotic neuroectodermal tumor of infancy (MNTI) is an increase in the urinary level of vanillylmandelic acid (VMA). (medscape.com)
Plain dental radiography, CT scanning, and MRI have been used to evaluate the content and the extent of melanotic neuroectodermal tumor of infancy (MNTI). (medscape.com)
Upon completion of the clinical examination of the patient and the imaging studies and urinalysis, the definitive diagnosis of melanotic neuroectodermal tumor of infancy (MNTI) is based on the histologic evaluation of a surgical specimen. (medscape.com)
[ 28 ] Additionally, entrapped developing tooth buds may be noted in the specimen as the melanotic neuroectodermal tumor of infancy (MNTI) grows in and around the odontogenic apparatus. (medscape.com)
Related tumors include Ewing sarcoma , peripheral primitive neuroectodermal tumor , neuroepithelioma, atypical Ewing sarcoma, and Askin tumor (tumor of the chest wall). (medscape.com)

This research has the potential to create smarter rational approaches to managing PGL tumors in patients. (mayo.edu)
OS appears to follow this model where OS cells with tumor initiating potential can be identified by expression of Stro1, CD117 and embryonic stem cell transcription factors such as Sox2, Nanog and Oct3/4. (tmc.edu)
In this study, we report that elevated levels of α-tubulin acetylation are a sufficient cause of metastatic potential in breast cancer. (nih.gov)
In the present study, we aimed to further examine the potential of formononetin in controlling angiogenesis and tumor cell invasiveness in human colon cancer cells and tumor xenografts. (spandidos-publications.com)
SOX9 regulates cancer stem-like properties and metastatic potential exposed cells. (cdc.gov)

There is also historical precedent for the concept, dating back to the 1800s when researchers observed tumor regression in patients infected with certain bacteria. (aacr.org)
In another recent study , published in Advanced Healthcare Materials , researchers engineered Escherichia coli to recruit radioactive molecules to tumor sites, thereby avoiding effects associated with traditional radiotherapy. (aacr.org)
Ludwig researchers find a key to fighting drug resistance in tumors. (ludwigcancerresearch.org)
Using miniature laboratory-grown tumors known as patient-derived organoids (PDOs) as models of CRC, the researchers also showed how expression of the immunotherapy target could be reactivated by blocking a particular molecular pathway, and so potenitally resensitize the tumors to treatment. (genengnews.com)
They identified CEA expression as a major determinant of cibisatamab sensitivity as only cell lines expressing moderate to high CEA levels were susceptible to T cell mediated killing," the researchers commented. (genengnews.com)
To investigate CRC resistance to cibisatamab sensitivity in a relevant laboratory model the researchers generated mini-tumor PDOs from biopsy samples taken from eight CRC patients, including seven with treatment-resistant metastatic CRC, and another with treatment naïve primary CRC, and co-cultured the organoids together with the CD8 T cells. (genengnews.com)
Taken together, CEA expression was frequently heterogeneous, showing a bimodal pattern in 50% of PDOs which has not been observed in cancer cell lines, and this phenomenon was associated with poor susceptibility to cibisatamab treatment," the researchers stated. (genengnews.com)

The incidence of renal cell carcinoma (RCC) is high, and its outcomes remain poor. (biologists.com)
Metastatic clear cell renal cell carcinoma is a disease without a comprehensive animal model for thorough preclinical investigation. (jove.com)
Clear cell renal cell carcinoma or CCRCC, is the most common subtype of kidney cancer. (jove.com)
Squamous cell carcinoma is the most prevalent histologic subtype of vulvar cancer, accounting for the majority of cases. (bvsalud.org)

Many late-stage, metastatic tumors contain the estrogen receptor but fail to respond to tamoxifen. (ludwigcancerresearch.org)

When ingested by the patient, the modified bacteria are thought to colonize primary and metastatic tumors and express IL-2, which in turn, recruits cancer-killing immune cells to the tumor sites. (aacr.org)
MCTS can mimic metastatic microtumors, which form when cancer cells spread from the site of the primary tumor via intravasation into the blood circulation and via extravasation into the parenchyma of an organ. (corning.com)
Metastatic disease to the lungs is the primary cause of death for patients with pediatric osteosarcoma (OS). (tmc.edu)
The cells start their journey by leaving the primary tumor . (cancer.gov)
To overcome these obstacles, we designed a functional microRNA (miR)-based live-cell reporter assay to identify highly tumorigenic cells in xenotransplants of primary Ewing sarcoma (EwS) 3D cultures. (visualsonics.com)
These results have clinical relevance for the understanding of the differences between primary tumours and their metastatic counterparts. (petrocollapse.org)
In Splitomicin this study, we used a paired colon cancer cell line model derived from a single patient, representative of the primary tumour (SW480) and its lymph node metastisis (SW620) (22). (petrocollapse.org)
In an analysis of more than 140 matched primary and metastatic tumors from patients, we found that acetylation was maintained and in many cases increased in lymph node metastases compared with primary tumors. (nih.gov)

The present invention provides a simple and robust human liver cell-based system in which persistent hepatitis C infection, persistent hepatitis B infection or ethanol exposure induces a clinical Prognostic Liver Signature (PLS) high-risk gene signature. (sumobrain.com)
This is a Phase 3 clinical study, which aims to evaluate the effectiveness of an investigational drug called pexidartinib for the treatment of certain tumors for which surgical removal could cause more harm than good. (stanford.edu)
Cibisatamab has shown promising results in early clinical trials, but some tumors are resistant to therapy, and studies in cancer cell lines have suggested that the effectiveness of cibisatamab is dependent upon expression of CEA on the tumor cells. (genengnews.com)
These cells often contain the melanin pigment that gives the MNTI its blue-black clinical appearance. (medscape.com)
These tumors are treated similarly on the basis of their clinical presentation (eg, metastatic or localized) rather than their histologic subtype (see the image below). (medscape.com)

Spheroids are an important part of this strategy, as they can be grown from cells harvested from tumor biopsies. (corning.com)

Metastatic treatment remains a challenge in oncology. (corning.com)
MRI scans will be used to evaluate the response of the tumors to the treatment. (stanford.edu)
Aggressive breast cancer is a highly heterogeneous disease caused by a variety of distinct cell-intrinsic genetic alterations in mammary epithelial cells, leading to vastly heterogenic disease manifestation in individual patients and predominantly affecting patient prognosis and treatment options [ 14 ]. (nature.com)
The results indicate that TTFields alone and in combination with paclitaxel and doxorubicin effectively reduce the viability of both wild type and MDR cell sub-lines and thus can potentially be used as an effective treatment of drug resistant tumors. (biomedcentral.com)
T cell therapy represents an emerging and promising modality for the treatment of both infectious disease and cancer. (biomedcentral.com)
Insights into future directions and challenges for the appropriate development of biomarkers to evaluate both product bioactivity and treatment efficacy of T cell therapy trials. (biomedcentral.com)
Tamoxifen is a mainstay of treatment for many women with breast cancer, but when the drug stops working, tumors can progress rapidly. (ludwigcancerresearch.org)
Initial tests with the new system first confirmed that, "as anticipated," while organoids expressing high levels of CEA (CEA hi ) were highly sensitive to treatment with CD8 T cells and cibisatamab, CEA lo PDOs that expressed only low levels of the glycoprotein were resistant to therapy. (genengnews.com)
Tumors evolve in response to treatment," said Haber. (nfcr.org)
We also discovered that the invasiveness of metastatic colon cancer cells was alleviated following drug treatment. (spandidos-publications.com)
The goals of surveillance imaging after the treatment of urothelial cancer of the urinary bladder are to detect new or previously undetected urothelial tumors, to identify metastatic disease. (bvsalud.org)

The molecular mechanisms underlying the transition from localized tumor to hormone-refractory metastatic PCa remain largely unknown, and their identification is key for predicting prognosis and targeted therapy. (jci.org)
1 It has been found that exosomes can mediate local and systemic cell communication with transmitting signals from one cell to another on the molecular level. (dovepress.com)
PeproTech's CHO cell-derived Recombinant Human MPF is the 250 amino acid-length fragment of the Mesothelin sequence, and has a calculated molecular weight of approximately 26.7 kDa. (peprotech.com)
Studded with microposts coated, or "functionalized," with antibodies designed to seize CTCs in mid-flow, the captured cells can not only be confirmed as tumor-related, but also counted and further analyzed in a variety of ways including molecular characterization. (nfcr.org)

However, all these mouse strains display residual immune functions, in particular, natural killer (NK) cells that can inhibit tumor formation and metastatic spread of cancer cells ( 20 , 21 ). (iiarjournals.org)

Exposure of cancer cells to chemotherapeutic agents may result in reduced sensitivity to structurally unrelated agents, a phenomenon known as multidrug resistance, MDR. (biomedcentral.com)
Multidrug resistance (MDR) [ 1 ] is encountered when cancer cells are exposed to chemotherapeutic agents for a few replication cycles. (biomedcentral.com)
Three decades of multidrug resistance research have identified a number of mechanisms by means of which cancer cells elude the effects of chemotherapeutic agents. (biomedcentral.com)

These results suggest that DARPP-32 restricts the migration of breast epithelial cells via both a transcription dependent mechanism that involves CREB and a transcription independent mechanism affecting FAK. (lu.se)
Differential gene regulation in human small airway epithelial cells grown in monoculture versus coculture with human microvascular endothelial cells following multiwalled carbon nanotube exposure. (cdc.gov)

MCTS mimic many of the features that tumors exhibit in the human body, including extracellular matrix (ECM) deposition between cells, gradients in nutrient concentration, and strong cell-cell junctions. (corning.com)
However, ST6GAL1 is also released into the extracellular milieu and extracellularly remodels cell surface and secreted glycans. (nature.com)
We hypothesize that ST6GAL1 action is the combined effect of natively expressed sialyltransferase acting cell-autonomously within the ER-Golgi complex and sialyltransferase from extracellular origins acting extrinsically to remodel cell-surface glycans. (nature.com)

RR demonstrated a 90% inhibition rate of mutagenicity in Ungernia victoris cultured cells. (doctorschar.com)
DARPP-32 mediated inhibition of cell migration proved to be dependent on DDR1 expression, consolidating a functional relevance of the interaction between DARPP-32 and DDR1. (lu.se)
In addition, we found that Wnt-5a could directly trigger a cAMP response that resulted in phosphorylation of DARPP-32 and stimulation with Wnt-5a was necessary for DARPP-32 mediated inhibition of cell migration in wound healing assay. (lu.se)

Reactivation may occur in patients treated with immunosuppressants such as tumor necrosis factor (TNF)-alpha antagonists (eg. (msdmanuals.com)

Anatomy23

Organisms11

Diseases90

Chemicals and Drugs40

Analytical, Diagnostic and Therapeutic Techniques and Equipment51

Phenomena and Processes47

Disciplines and Occupations1

Information Science5

Health Care9

Microenvironment17

Metastasis40

Progression14

Colorectal cancer5

Proliferation12

Vitro15

Solid tumors4

Heterogeneity3

Melanoma17

Treat tumors3

Therapeutic6

Lung15

Malignant4

Behavior4

Mouse tumor-bearin1

Circulating Tumor Cells6

Recurrence3

Tissues2

Patients with metastatic1

Apoptosis2

Types of tumors1

Vivo5

Highly metastatic1

Subcutaneous3

CSCs11

Anti-tumour2

Aggressive tumor cell1

Survival3

Biomarker1

Prostate3

Lymphocytes1

Adhesion4

Findings2

Cellular2

Phenotype1

Murine3

Ovarian cancer6

Chemotherapy5

Renal cancer1

Mice8

Significantly5

Motility3

Cancers4

Invasive2

Promotes tumor1

Tissue4

Growth8

Tumorigenesis2

Therapies2

Mutations4

Neuroectodermal7

Potential5

Researchers7

Carcinoma4

Estrogen receptor1

Primary8

Clinical5

Biopsies1

Treatment11

Molecular4

Inhibit1

Chemotherapeutic agents3

Epithelial cells2

Extracellular3

Inhibition3

Necrosis1