Electrophoresis-massTime-of-flight maEnantiomersUrineChromatographyAnionicMetabolomicsChromatographicFused-silicaProfilingQuantificationElectrospray ionization mNutrientsMetabolomeUrinaryChiralIdentificationConcentrationsIonicAccumulationDetectionMetabolismBiologicalAnalysisPhosphateAminoMusclesHPLCMethodHumanExposureMassSamplesBloodDiameterCompromiseMajorStudyLarge
Electrophoresis-mass4
- Capillary electrophoresis-mass spectrometry (CE-MS) represents a high efficiency microscale separation platform for untargeted profiling of polar/ionic metabolites that is ideal for volume-restricted biological specimens with minimal sample workup. (mcmaster.ca)
- To test this hypothesis, we used a rat model of endurance exercise, a microwave irradiation for accurate detection of glycogen in the brain (the cortex, hippocampus, and hypothalamus), and capillary electrophoresis mass spectrometry-based metabolomics to observe the comprehensive metabolic profile of the blood. (frontiersin.org)
- Sheath-flow capillary electrophoresis-mass spectrometry (CE-MS) has emerged as a new tool for comprehensive analysis of charged metabolites. (elsevierpure.com)
- Hirayama, A , Tomita, M & Soga, T 2012, ' Sheathless capillary electrophoresis-mass spectrometry with a high-sensitivity porous sprayer for cationic metabolome analysis ', Analyst , vol. 137, no. 21, pp. 5026-5033. (elsevierpure.com)
Time-of-flight ma3
- To monitor the physiological state of yeast cropped from this fermentation test, we estimated intracellular metabolite concentrations using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS). (asbcnet.org)
- Serum metabolites were identified and quantified by capillary electrophoresis coupled with time-of-flight mass spectrometry. (edu.au)
- Here, we present non-targeted analysis using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) for the profiling of extracellular metabolites released during macrophage activation. (elsevierpure.com)
Enantiomers4
- Using capillary zone electrophoresis with a phosphate buffer at pH 2.5 containing 30 mM (2-hydroxypropyl)-beta-cyclodextrin as chiral selector, the simultaneous separation of the enantiomers of 3,4-methylenedioxymethamphetamine (MDMA or Ecstasy) and its two metabolites 4-hydroxy-3-methoxymethamphetamine (HMMA) and 3,4-methylenedioxyamphetamine (MDA) in human urine is reported. (erowid.org)
- The metabolism of the enantiomers of the two metabolites showed interindividual differences. (erowid.org)
- Analytical study of different species by separation techniques coupled to optical detectors and mass spectrometry in order to perform quantitative determinations of these species in different real matrices, as well as the determination of their metabolites, enantiomers and speciation. (uclm.es)
- Enantiomeric separation and quantification of warfarin enantiomers and clinically important major hydroxylation metabolites are essential for drug interaction studies and phenotypic characterization of CYP2C9 and CYP3A4, the major Cytochrome P450 (CYP) enzymes involved in warfarin metabolism. (austinpublishinggroup.com)
Urine4
- A micellar electrokinetic capillary chromatographic method for determining anionic catecholamine metabolites from patient urine samples was established. (vtt.fi)
- A diverse range of acidic metabolites in human urine were reliably measured by MSI-CE-MS via serial injection of seven urine samples within a single run, including organic acids, food-specific markers, microbial-derived compounds and over-the-counter drugs as their sulfate and glucuronide conjugates. (mcmaster.ca)
- Capillary gas chromatographic method for mandelic and phenylglyoxylic acids in urine. (cdc.gov)
- When the method was applied to the analysis of cationic metabolites obtained from human urine, there was a 10-fold increase in the number of detected peaks compared with conventional methods. (elsevierpure.com)
Chromatography2
- Ethyl-acetate extracts were prepared and treated with N,O-bis-(trimethylsilyl)- trifluoroacetamide and isooctane prior to capillary gas chromatography with flame ionization detection. (cdc.gov)
- I develop analytical methods based on chromatography and mass spectrometry for analysis of metabolites in various tissue. (lu.se)
Anionic2
- Despite these advantages, the long-term stability of CE-MS remains a major obstacle hampering its widespread application in metabolomics notably for routine analysis of anionic metabolites under negative ion mode conditions. (mcmaster.ca)
- In this work, multisegment injection (MSI)-CE-MS was used as multiplexed separation platform for high throughput profiling of anionic metabolites when using optimized buffer conditions to prevent polyimide degradation. (mcmaster.ca)
Metabolomics4
- The combination of high-resolution LC-MS-based untargeted metabolomics with stable isotope tracing provides a global overview of the cellular fate of precursor metabolites. (acs.org)
- Metabolomics detected 186 metabolites in the plasma, and 110 metabolites changed significantly during and following exhaustive exercise. (frontiersin.org)
- These new and provocative scientific perspectives on metabolism are being driven by enabling advances in metabolomics - the study of metabolites that are transformed during metabolism and visualised as functional maps of cellular processes. (ddw-online.com)
- We use metabolomics, i.e. the determination of as many metabolites as possible in a tissue, to study metabolic diseases. (lu.se)
Chromatographic2
Fused-silica2
- Herein, we report for the first time that commonly used ammonia containing buffers compatible with electrospray ionization (ESI)-MS can compromise the integrity of fused-silica capillaries via aminolysis of their outer polyimide coating. (mcmaster.ca)
- The assay described is based upon enzymatic hydrolysis of conjugated HMMA (major urinary metabolite) and solid-phase extraction followed by injection of a few nL of the extract onto a 50 microm internal diameter (ID) fused-silica capillary of 60 cm length. (erowid.org)
Profiling1
- In order to build a comprehensive metabolite database for HAF, hyphenated NMR has been used, for the first,time, for systematic HAF profiling. (ua.pt)
Quantification3
- This methodology enables detection of putative metabolites from biological samples and simultaneous quantification of the pattern and extent of isotope labeling. (acs.org)
- Though alterations in the NAD + to nicotinamide ratio and the NAD + to NADH ratio are anticipated by models to account for the effects of calorie restriction, the nature of a putative change in NAD + metabolism requires analytical definition and quantification of the key metabolites. (biomedcentral.com)
- The controversy triggered several environmental and epidemiologic studies, and it generated needs for sensitive and selective analytical methods for the quantification of atrazine, atrazine metabolites, and degradation or hydrolysis products. (cdc.gov)
Electrospray ionization m1
- Here, we report a sheathless capillary electrophoresis-electrospray ionization-mass spectrometry method for cationic metabolome analysis. (elsevierpure.com)
Nutrients2
- It is a metabolically active tissue that requires a continuous flow of nutrients and metabolites. (azooptics.com)
- Moreover, the lining of the capillaries is attenuated on the side closest to the photoreceptor cells--another indication that their fundamental purpose is the rapid and efficient delivery of nutrients to the photoreceptor layer. (arn.org)
Metabolome1
- Metabolism is a complex network involving thousands of enzymes and metabolites - the metabolome. (lu.se)
Urinary2
- The method developed here helped distinguish between microdose intake of ( E / Z )‐clomiphene citrate and consumption of clomiphene‐containing eggs by the urinary pattern of four mono‐hydroxylated clomiphene metabolites. (researchgate.net)
- The other setting was the For accurate estimation of infant expo- neonatal intensive care unit, which was cho- sure, measurement of urinary levels of coti- sen to assess in utero exposure of neonates nine, the proximate metabolite of nicotine, to tobacco smoke. (who.int)
Chiral2
- The possibility to enhance resolution to infinite value in chiral capillary electrophoresis is attained as soon as the apparent mobility of one enantiomer becomes opposite to the other. (unboundmedicine.com)
- With tramadol and its phase I metabolites selected as model compounds, the HS-gamma-CD was found to be the most appropriate chiral selector. (unboundmedicine.com)
Identification1
- The identification of metabolites allows for the expansion of possible targets for anti-doping analysis. (researchgate.net)
Concentrations1
- The concentrations of the catecholamine metabolites in the studied patient urines varied from 0.186 to 76.4 μg/ml. (vtt.fi)
Ionic1
- In order to better understand the interaction mechanism between the selected CD and the analytes, the classical affinity capillary electrophoresis method, although not fully satisfactory because of ionic strength variations within a series of mobility shift measurements, was applied to estimate complexation constants and complex mobilities. (unboundmedicine.com)
Accumulation2
- There was greater accumulation of secondary metabolites and upregulation of secondary metabolite-related genes after BNS. (nature.com)
- The accumulation of metabolites in the culture supernatant was monitored. (elsevierpure.com)
Detection3
- A novel approach for detection, analysis, and characterization of low-abundant herbicide metabolites is reported here. (chromatographyonline.com)
- Hyphenated NMR has allowed the rapid detection of similar to 60 metabolites in HAF, some of which are not detectable by standard NMR due to low abundance (mu M) and signal overlap thus enabling an extended metabolite database to be built for HAF. (ua.pt)
- Thus: sensitive and specific analytical methods to quantify warfarin and its clinically significant metabolites in various matrices: especially human plasma: may help to improve the understanding of warfarin pharmacology and detection of potential warfarin-drug/diet interactions. (austinpublishinggroup.com)
Metabolism1
- Labeling of Trypanosoma brucei cell cultures with 50% uniformly 13C-labeled glucose demonstrated incorporation of glucose-derived carbon into 187 of 588 putatively identified metabolites in diverse pathways including carbohydrate, nucleotide, lipid, and amino acid metabolism. (acs.org)
Biological1
- Analysis of active principles of clinical and/or animal health interest and their metabolites in biological fluids. (uclm.es)
Analysis2
- It is also likely to have applications in the analysis of biofluids containing many different small molecule metabolites. (chemistryworld.com)
- Based on this phenomenon, we developed a portable capillary-driven microfluidic device that separates blood microsamples collected from finger-pricks and delivers 2 µL of metered serum for bench-top analysis. (researchgate.net)
Phosphate1
- Labeling patterns confirmed the metabolic pathways responsible for the biosynthesis of many detected metabolites, and labeling was detected in unexpected metabolites, including two higher sugar phosphates annotated as octulose phosphate and nonulose phosphate. (acs.org)
Amino1
- Under optimized conditions, 53 cationic metabolites, including amino acids and their derivatives, amines, nucleic acids and small peptides, were successfully separated and selectively detected with a time-of-flight mass spectrometer. (elsevierpure.com)
Muscles1
- Insulin resistance is associated with the capillary permeability of thigh muscles in patients with type 2 diabetes. (medscape.com)
HPLC1
- Whole-body extracts of 50 specimens were fractionated by HPLC and each fraction was analysed by capillary NMR. (chemistryworld.com)
Method2
- This method assesses human exposure to select pesticide metabolites of non-persistent pesticides. (cdc.gov)
- Proteomic and metabolomic analyses of the microsamples of 283 proteins and 1351 metabolite features was consistent with samples generated via a conventional centrifugation method. (researchgate.net)
Human1
- 10 S )-10-hydroxywarfarin (the CYP3A4 metabolite) in human plasma. (austinpublishinggroup.com)
Exposure1
- Unlike organic solvent swelling effects, this chemical process occurs under aqueous conditions that is dependent on ammonia concentration, buffer pH, and exposure time resulting in a higher incidence of capillary fractures and current errors during extended operation. (mcmaster.ca)
Mass1
- Now, US researchers from Cornell University and the State University of New York have improved the mass sensitivity further by employing a capillary NMR probe. (chemistryworld.com)
Samples1
- Results: Based on m/z values and migration times, 136 metabolites were identified in serum samples. (edu.au)
Blood3
- citation needed] The loop of Henle is supplied by blood in a series of straight capillaries descending from the cortical efferent arterioles. (wikipedia.org)
- Glomerular filtration - Filtrate is made as the blood is filtered through a collection of capillaries in the nephron called glomeruli. (healthpages.org)
- Most relevant associations of blood metabolites with the most accepted phenotypes described for the disease. (archbronconeumol.org)
Diameter1
- These capillaries are much larger than standard capillaries being between 18 50 microns in diameter. (arn.org)
Compromise1
- The fibrin cuff theory postulates that because of chronic venous compromise, fibrinogen leaks from the capillaries. (medscape.com)
Major3
- A major advantage of the capillary NMR system is the very low level of artifacts in the spectra, which is probably caused by the high sample concentration and the small volume that contrive together to reduce solvent peaks. (chemistryworld.com)
- UDP-Glucuronosyltransferases (UGTs) are important in the elimination of most xenobiotics, including 5-( p -hydroxyphenyl)-5-phenylhydantoin (HPPH), the major, reputedly nontoxic, metabolite of the anticonvulsant drug phenytoin. (aspetjournals.org)
- The glucuronidation and elimination of endogenous compounds ( e.g. , bilirubin) and xenobiotics, including HPPH, the major, para -hydroxylated metabolite of the anticonvulsant drug phenytoin (diphenylhydantoin) ( Butler, 1957 ), are catalyzed by a superfamily of membrane-bound isozymes known collectively as UGTs ( Dutton, 1980 ). (aspetjournals.org)
Study2
- This is the first study to produce a broad picture of plasma metabolite changes due to endurance exercise-induced brain glycogen supercompensation. (frontiersin.org)
- Metabolites as Risk Factors for Diabetic Retinopathy in Patients with Type 2 Diabetes: a 12-year Follow-up Study. (medscape.com)
Large1
- It is delivered by a large capillary network that forms an orderly pattern throughout the muscle fibers. (azooptics.com)