• In nonphotosynthetic organisms, the FNR primarily works in reverse to provide reduced ferredoxin for various metabolic pathways. (wikipedia.org)
  • Hence, we suggest that, by designing highly potent and specific inhibitors of TryR enzyme, inhibition of T[SH] 2 reduction and overall inhibition of most of the downstream pathways including Fe/S protein activation reactions, can be accomplished. (frontiersin.org)
  • The question remains how is the partitioning of electrons between the various energy-conserving and -dissipating pathways achieved. (nature.com)
  • One such class of pathways is involved in the biogenesis of Iron-Sulfur Clusters (ISC). (biomedcentral.com)
  • We propose and apply an iterative in silico procedure for predictive reconstruction of the network topology of metabolic pathways. (biomedcentral.com)
  • Increasing amounts of data that can be mined for information about how proteins in cells assemble as metabolic pathways, signal transduction pathways, and gene circuits, are generated each day. (biomedcentral.com)
  • Iron-Sulfur Cluster biogenesis) or complex pathways with an unclear reaction and regulation network, (e. g. cell cycle). (biomedcentral.com)
  • The alteration of metabolic pathways is a critical strategy for cancer cells to attain the traits necessary for metastasis in disease progression. (biomed.news)
  • We hope to unravel new redox-regulated cellular processes or signaling pathways in poplar and more generally in plants which are controlled by redox reactions such as thiol-disulfide exchanges and understand how the functions of these proteins are controlled at the cellular level by TRX and GRX. (hal.science)
  • In this context, the intervention of the TRX and glutathione (GSH)/GRX reducing systems in the sulfur signaling/trafficking pathways is examined as both the TRX and GSH/GRX systems might modulate STR function and in particular be central to the formation of hydrogen sulfide, a molecule susceptible to trigger redox signalling cascades in several physiological situations. (hal.science)
  • To identify determinants of mETC function, we screened a genome-wide human CRISPRi library under oxidative metabolic conditions with selective inhibition of mitochondrial Complex III and identified OCIA domain-containing protein 1 (OCIAD1) as a Complex III assembly factor. (biorxiv.org)
  • Redox post-translational modifications of proteins** In addition to performing structure-function analyses of thioredoxin (TRX) and glutaredoxin (GRX) family members which control most of the reversible oxidative modifications of protein cysteinyl residues, we are characterizing proteins of unknown function possessing one or several conserved CXXC motifs known to be particularly suited for disulfide bond formation but also other redox modifications. (hal.science)
  • Using inductively coupled plasma mass spectrometry, UV-visible absorption, EPR, and Mössbauer spectroscopies, we characterized nsp13 as an iron-sulfur (Fe-S) protein that ligates an Fe4S4 cluster in the treble-clef metal-binding site of its zinc-binding domain. (bvsalud.org)
  • Exposure of the protein to the stable nitroxide TEMPOL oxidizes and degrades the cluster and drastically diminishes unwinding activity. (bvsalud.org)
  • Along with its most important role as central reductant, T[SH] 2 have also been assumed to regulate the activation of iron-sulfur cluster proteins (Fe/S). Fe/S clusters are versatile cofactors of various proteins and execute a much broader range of essential biological processes viz. (frontiersin.org)
  • Although, several Fe/S cluster proteins and their roles have been identified in Leishmania, some of the components of how T[SH] 2 is involved in the regulation of Fe/S proteins remains to be explored. (frontiersin.org)
  • In pursuit of this aim, a systems biology approach was undertaken to get an insight into the overall picture to unravel how T[SH] 2 synthesis and reduction is linked with the regulation of Fe/S cluster proteins and controls the redox homeostasis at a larger scale. (frontiersin.org)
  • This has also caused an elevated level of free radicals which apparently affected the activation of Fe/S cluster proteins. (frontiersin.org)
  • Using the Iron Sulfur cluster biogenesis in S. cerevisiae as a test case we indicate how this procedure can be used to analyze and validate the network model against experimental results. (biomedcentral.com)
  • Yersinia genomes had a similar global partition of protein functions, as measured by the distribution of Cluster of Orthologous Groups families. (biomedcentral.com)
  • Involved in the biogenesis and/or repair of oxidatively damaged Fe S clusters. (or.jp)
  • Iron-sulfur (Fe-S) clusters are essential cofactors present in all known forms of life and required by hundreds of proteins to perform their function. (curefa.org)
  • CF27-specific gene clusters which have been analyzed were expressed by both ferrous iron-grown and sulfur-attached cells, indicating that they are not pseudogenes and may play a role in both situations. (frontiersin.org)
  • After focusing for some time on the capacity of several plant GRXs to assemble \[2Fe-2S\] centers into homodimers or into heterodimers with BOLA proteins, we extended our research interest to the functional characterization of the associated protein families (NFUs, SUFA/ISCAs, IBA57s, HCF101/INDH) assumed to participate in the transfer of these Fe-S clusters together with GRX and BOLA. (hal.science)
  • This functional analysis will combine plant genetics and physiology, molecular and structural biology and biochemistry approaches to determine whether these proteins assemble different types of Fe-S clusters and have specific interaction partners and what are the physiological and metabolic consequences of deleting these genes for plant development and physiology. (hal.science)
  • 96% of genes in KN400 had clear orthologs with conserved synteny in PCA. (biomedcentral.com)
  • Most of the remaining genes were in regions of genomic mobility and were strain-specific or conserved in other Geobacteraceae , indicating that the changes occurred post-divergence. (biomedcentral.com)
  • Genes known to be involved in electron transport, those used in the metabolic cell model, and those that exhibit changes in expression during growth in microbial fuel cells were examined in detail. (biomedcentral.com)
  • miRNAs are either expressed from independent transcriptional units or derive from introns of protein-coding genes or exons or introns of long ncRNAs. (biomedcentral.com)
  • In the last steps of this process transfer of energy-conserving electrons beyond photosystem I (PSI) is performed by a small iron-sulphur protein ferredoxin (Fd). (nature.com)
  • Binding of the enzyme to the thylakoid membrane involves a polyproline type II helix created between two FNR monomers and several proline rich integral membrane proteins. (wikipedia.org)
  • FNR is a soluble protein that is found both free in the chloroplast stroma and bound to the thylakoid membrane. (wikipedia.org)
  • The binding of the FNR to the integral membrane proteins on the thylakoid membrane is enhanced under acidic conditions, so recruitment and binding of FNR to the thylakoid membrane may be a method of storing and stabilizing the enzyme in the dark when photosynthesis is not occurring. (wikipedia.org)
  • Our data suggest that H2 O2 -mediated signaling is initiated only in close proximity to mitochondria and under specific metabolic conditions. (biomed.news)
  • Mitochondria are central hubs for cellular metabolism, coordinating a variety of metabolic reactions crucial for human health. (biomed.news)
  • This enzyme belongs to the family of oxidoreductases, that use iron-sulfur proteins as electron donors and NAD+ or NADP+ as electron acceptors. (wikipedia.org)
  • I am a biochemist interested in basic plant science processes with a particular research focus on structure-function analysis of oxidoreductases and on redox post-translational modifications of proteins. (hal.science)
  • The same questions and strategies apply to sulfurtransferases, enzymes that catalyze the transfer of a sulfur atom from sulfur donors to nucleophilic sulfur acceptors (trans-persulfidation) by forming themselves persulfides. (hal.science)
  • Multiple membrane-bound heterodisulfide reductase (DsrMK) could promote both energy-conserving and non-energy-conserving menaquinol oxidation. (hindawi.com)
  • has been shown to consist of at least seven species, all of which derive energy from the oxidation of elemental sulfur and reduced inorganic sulfur compounds (RISCs) to support their growth. (frontiersin.org)
  • As a test case, we reconstruct the topology of the reaction and regulatory network for the mitochondrial ISC biogenesis pathway in S. cerevisiae . (biomedcentral.com)
  • A putative role for frataxin in directly regulating mitochondrial iron import is discarded from our analysis, which agrees with also published experimental results. (biomedcentral.com)
  • We find that OCIAD1 is an inner mitochondrial membrane protein that forms a complex with supramolecular prohibitin assemblies. (biorxiv.org)
  • Here, we review mechanisms of mitochondrial gene expression with a focus on the recent findings in the field of mammalian mtDNA transcription and disease phenotypes caused by defects in proteins involved in this process. (biomed.news)
  • Mai, H., Baby, D., & Bauer, P. (2023) Black sheep, dark horses and colorful dogs: A review on the current state of the Gene Ontology with respect to iron homeostasis in Arabidopsis thaliana . (hhu.de)
  • For our research projects, we employ biochemical, biophysical, spectroscopic and structural biology approaches with *Populus trichocarpa* and *Arabidopsis thaliana* as model organisms to explore (i) the roles of the antioxidant/detoxification systems in the physiology of plants subject to environmental constraints and (ii) the maturation and roles of iron-sulfur proteins in plant organelles. (hal.science)
  • Cysteine, a thiol-containing amino acid, is crucial for the synthesis of sulfur-containing biomolecules that control multiple essential cellular activities. (biomed.news)
  • Dynamic adaptation of cytosolic thioredoxin reductase levels during metabolic changes results in improved H2 O2 handling and explains previously observed differences between cell types. (biomed.news)
  • Substantial sequence differences (at least 12 non-synonymous SNP/kb) were found in 3.6% of the orthologs, and this set was enriched in cytochromes and integral membrane proteins. (biomedcentral.com)
  • FNR is recruited to thylakoid membranes via integral membrane thylakoid rhodanase-like protein TROL. (nature.com)
  • The improvement in external electron transfer in the KN400 strain does not appear to be due to novel gene acquisition, but rather to changes in the common metabolic network. (biomedcentral.com)
  • The more than likely absence of known mechanistic and kinetic data for each of the individual proteins in a novel pathway hinders the process of translating network topology into a mathematical model. (biomedcentral.com)
  • The miRNA duplexes are loaded into an Argonaute protein in the miRNA-induced silencing complex (miRISC) and rapidly unwound. (biomedcentral.com)
  • Pre-miRNAs are exported to the cytoplasm by Exportin-5 and processed further by Dicer, to ~22 nt double-stranded miRNA duplexes that are loaded into an Argonaute protein in the miRISC and rapidly unwound. (biomedcentral.com)
  • The mechanistic data imply (i) that BesD may have evolved from a hydroxylase ancestor either relatively recently or under weak selective pressure for efficient chlorination and (ii) that acquisition of its activity may have involved the emergence of linkage between l-Lys binding and chloride coordination following the loss of the anionic protein-carboxylate iron ligand present in extant hydroxylases. (bvsalud.org)
  • HOs are the key-limiting enzymes in heme degradation leading to carbon monoxide (CO), ferrous iron, and biliverdin products. (encyclopedia.pub)
  • Heme is degraded by heme oxygenases (HOs), generating biliverdin, carbon monoxide, and ferrous iron (Fe 2+ ). (encyclopedia.pub)
  • Ferrous iron (Fe 2+ ) possesses pro-oxidant activity. (encyclopedia.pub)
  • However, activation of heme oxygenase-1 (HO-1) can upregulate ferritin expression, which binds to ferrous iron and detoxifies its pro-oxidant effect. (encyclopedia.pub)
  • Ferrous iron increases ROS generation via the Fenton reaction. (encyclopedia.pub)
  • Halide, 2OG, and (lastly) O2 all coordinate directly to the cofactor to initiate its conversion to a cis-halo-oxo-iron(IV) (haloferryl) complex, which abstracts hydrogen (Hâ ¢) from the non-coordinating prime substrate to enable radicaloid carbon-halogen coupling. (bvsalud.org)
  • Environmental sequences of ten novel plastid lineages and structural innovations in plastid proteins confirm that plastids in apicomplexans and their relatives are widespread and share a common, photosynthetic origin. (elifesciences.org)
  • Lichtblau DM , Schwarz B , Baby D , Endres C, Sieberg C and Bauer P (2022) The Iron Deficiency-Regulated Small Protein Effector FEP3/IRON MAN1 Modulates Interaction of BRUTUS-LIKE1 With bHLH Subgroup IVc and POPEYE Transcription Factors. (hhu.de)
  • Transcription of mtDNA is not only essential for the biogenesis of the OXPHOS system, but also generates RNA primers necessary to initiate mtDNA replication. (biomed.news)
  • This occurs through the downregulation of methylmalonyl coenzyme A epimerase (MCEE), mediated by an extracellular signal-regulated kinase 2-driven transcription factor Sp1/early growth response protein 1 transcriptional switch driven by metastatic signalling at its promoter level. (biomed.news)
  • For example, the involvement of proteins X, Y and Z in a process does not elucidate if X catalyzes a reaction that produces a substrate for another reaction catalyzed by Z or by Y, or if X modulates Y or Z activity. (biomedcentral.com)
  • Earliest agronomist not considered Si as an essential element as N, P and K for plant growth because of non-availability of positive evidence to show Si involvement in any metabolic activities of plants (Datnoff et al. (researchsquare.com)
  • events have receptors stimulated with students( GAGs), unregulated chains docking of a inactive actin, all of an been protein respiration cell a considerable subunits. (evakoch.com)
  • This activates signaling to the actomyosin cortex via nuclear envelope stretch-sensitive proteins, up-regulating cell contractility. (regenerativemedicine.net)
  • Nonstructural protein (nsp) 13 is a helicase required for viral replication. (bvsalud.org)
  • Datasets available for such tasks include the primary literature, large scale micro array experiments, whole genome two hybrid screenings, full genome sequences, and the patterns of conserved/non-conserved homologues and orthologues in them. (biomedcentral.com)
  • Heme oxygenases (HOs) act on heme degradation to produce carbon monoxide (CO), free iron, ferritin, and biliverdin. (encyclopedia.pub)
  • The spectrum of diseases caused by C. difficile is highly variable and depends not only on host factors but also, for the severe forms, on the level of toxin production (4). (studyres.com)
  • Plague is a zoonotic disease, endemic throughout the world, and highly infectious in humans. (asm.org)
  • Although human disease is rare, Y. pestis is dangerous and highly infectious and thus has been identified as having potential for use in bioterrorism or as a biological weapon. (asm.org)
  • Review: Role of the plant-specific calcium-binding C2-DOMAIN ABSCISIC ACID-RELATED (CAR) protein family in environmental signaling. (hhu.de)
  • HMOX2 is constitutively expressed to encode a 36 kDa HO-2 protein, mainly functioning to maintain the basal heme metabolism and may also play a role in inflammatory responses [ 4 ] . (encyclopedia.pub)
  • In addition, the mETC and the associated tricarboxylic acid (TCA) cycle support a network of metabolic functions. (biorxiv.org)
  • The achievement, at a global level, of a "healthy aging phenotype" defined as "the condition of being alive, while having highly preserved functioning metabolic, hormonal and neuroendocrine control systems at the organ, tissue and molecular levels" [ 10 ], is the most ambitious objective that modern science should achieve. (iospress.com)
  • Accepted August 23, 2012 ABSTRACT The catabolite control protein CcpA is a pleiotropic regulator that mediates the global transcriptional response to rapidly catabolizable carbohydrates, like glucose in Gram-positive bacteria. (studyres.com)
  • To achieve such a goal, strategies that combine the different theoretical and computational methods to identify proteins and generate a set of plausible alternative network topologies for the process of interest are needed. (biomedcentral.com)
  • Predictions regarding how proteins act in ISC biogenesis are validated by comparison with published experimental results. (biomedcentral.com)
  • Here, we found that nsp13 ligates iron, in addition to zinc, when purified anoxically. (bvsalud.org)
  • We found 453 single nucleotide polymorphisms in protein-coding regions, which were used to assess the evolutionary relationships of these Y. pestis strains. (asm.org)