• Differentiation
  • Type I diabetes therapy should potentially benefit from such differentiated cells: the search for alternatives to organ/islet transplantation strategies via stem cells differentiation is an ongoing task, significant goals having been achieved in most experimental settings (e.g. insulin production and euglycaemia restoration), though caution is still needed to ensure safe and durable effects in vivo. (springer.com)
  • Transformation of the iTr cells to the spherical arrangement stimulated expression of genes that mark differentiation of trophoblast cells and up-regulated 250 transcripts over the monolayer arrangement. (vt.edu)
  • markers
  • Immunofluorescence staining, real-time PCR, flow cytometry analysis, and multipotency assays showed that hWJ-MSC derived from 3D culture retained MSC surface markers and multipotency potential similar to 2D culture-derived cells. (biomedcentral.com)
  • The transduced equine cells did not express the ES marker NANOG, but did express the trophectoderm markers CDX2 and TFAP2A. (vt.edu)
  • possess
  • Moreover, recent reports evidenced that MSC possess immunomodulatory activities (acting on both innate and acquired immunity effectors) which should result in a reduction of the immunogenicity of transplanted cells, thus limiting rejection. (springer.com)
  • culture
  • MSC are obtainable in high numbers via ex vivo culture and can be differentiated towards insulin-producing cells (IPC). (springer.com)
  • Institutional review board approval was obtained for all cell culture procedures. (biomedcentral.com)
  • These cells also efficiently enhanced skin wound healing in vivo, in a manner comparable to that of hWJ-MSC obtained from 2D culture. (biomedcentral.com)
  • Based on the size of spheroids (283,07 μm ± 43,10 μm) we found that three weeks of culture was the best period to growth the UC-WJ cells on 3D dimension. (biomedcentral.com)
  • Taken together all results, it was possible to demonstrate an easy way to isolate and culture of bovine-derived UC-WJ cells under 2D and 3D serum-free condition, from fetal adnexa with a great potential in cell therapy and biotechnology. (biomedcentral.com)
  • Culture of the iTr cell in suspension resulted in formation of blastocyst-like spheres rather than solid cell aggregates indicative of ES and iPS cells. (vt.edu)
  • The iTr cells were transitioned to a feeder free monolayer culture. (vt.edu)
  • tissue
  • In mammalian tissue, cells connect not only to each other, but also support structures called the extracellular matrix (ECM). (biomedcentral.com)
  • expansion
  • Taken together, these modifications may lead to an impairment of both cell expansion ability and resistance to apoptosis, two hallmarks of aging cells. (biomedcentral.com)
  • Moreover
  • Moreover it has been proposed that MSC administration should be used to attenuate the autoimmune processes which lead to the destruction of beta cells. (springer.com)
  • unique
  • The generation of iTr cells by transduction of the Yamanaka reprogramming factors is not unique to equine cells. (vt.edu)
  • capable
  • However, this report marks the generation of the first equine trophoblast cell line capable of recapitulating early equine trophoblast development. (vt.edu)
  • expression
  • Molecular evaluation revealed the cells maintained expression of endogenous OSKM while the exogenous OSK transgenes were extinguished, but MYC was maintained. (vt.edu)