• Topics include biofilms, biofuels, cancer-suppressing compounds, stem cells, molecular aspects of dementia and diabetes, DNA repair, HIV and other viruses, drug-resistant bacteria and other microbes, plasma-membrane pumps, and new tools to study protein conformation (the precise folding that determines function). (calstatela.edu)
  • Docking to the plasma membrane prepares vesicles for rapid release. (rupress.org)
  • In Caenorhabditis elegans motor neurons, dense core vesicles dock at the plasma membrane but are excluded from active zones at synapses. (rupress.org)
  • Dense core vesicles are usually found in the cytoplasm and these cytoplasmic vesicles must presumably translocate to the plasma membrane before release ( Zupanc, 1996 ). (rupress.org)
  • SPRED1 recruits Nf1 from the cytosol to the plasma membrane where Ras resides, and Nf1 subsequently can downregulate GTP-bound Ras 14 . (nature.com)
  • We obtained more direct evidence through generating improved transmission electron microscopy (TEM) images and image averages of the rosette-type CSC, revealing the frequent triangularity and average cross-sectional area in the plasma membrane of its individual lobes. (osti.gov)
  • Of the 8 plasma membrane receptors for complement, only deficiencies of CR3 and CR4 due to CD18 deficiency have been described, known as leukocyte adhesion deficiency (LAD) type 1. (medscape.com)
  • MorphoSys , a biotechnology company focused on fully human antibodies, and Heptares Therapeutics , a developer of new medicines targeting G protein-coupled receptors (GPCRs), have signed an agreement to discover novel antibody therapeutics targeting GPCRs-membrane proteins involved in a broad range of biological processes and diseases. (centerwatch.com)
  • G-protein Coupled Receptors (GPCRs) comprise one of the largest families of signaling molecules with more than a thousand members currently predicted to exist. (thermofisher.com)
  • G-protein coupled receptors (GPCRs) are membrane proteins that transmit the effects of extracellular ligands to effect changes in the intracellular G-protein signaling system. (gauss-centre.eu)
  • secreted PLA2s have also been found to specifically bind to a variety of soluble and membrane proteins in mammals, including receptors. (nih.gov)
  • In biology, chemiosmosis refers to the process of moving ions (e.g. protons) to the other side of the membrane resulting in the generation of an electrochemical gradient that can be used to drive ATP synthesis. (roadlesstraveledstore.com)
  • A secondary structure model of PutP from Escherichia coli suggests the protein contains 13 TMs with the N terminus located on the periplasmic side of the membrane and the C terminus facing the cytoplasm. (embl.de)
  • The extent of the elevator-like movement of the transport domain is so large (~20 Å in Glt Tk ) that HP2 acts as a gating element both on the extracellular and the intracellular side of the membrane. (nature.com)
  • Reduction in the intensity of tryptophan emission spectra of mitochondrial membrane proteins in presence of an increasing concentration of a ligand can be used to study the interaction of insecticides/drugs with the intact mitochondria. (cgiar.org)
  • It has been shown that alpha-synuclein is localized in the inner membrane of mitochondria, and that the inhibitory effect of alpha-synuclein on complex I activity of the mitochondrial respiratory chain is dose-dependent. (wikipedia.org)
  • de Marcos-Lousa C, P Sideris D, Tokatlidis K. (2006) Translocation of mitochondrial inner-membrane proteins: conformation matters. (forth.gr)
  • The removal of protons from the matrix and deposition of protons in the intermembrane space creates a concentration difference of protons across the inner membrane. (roadlesstraveledstore.com)
  • It resides in the inner membrane of Escherichia coli and serves as protective safety valve in the event of osmotic shock. (usda.gov)
  • Characterization of the two conformations adopted by the T3 SS inner-membrane protein PrgK. (ubc.ca)
  • Copper binding and conformation of the N-terminal octarepeats of the prion protein in the presence of DPC micelles as membrane mimetic. (mpg.de)
  • Although the role of abnormal prion protein (PrP) conformation in generating infectious brain diseases (transmissible spongiform encephalopathy) has been recognized, the function of PrP in the normal brain remains mostly unknown. (amrita.edu)
  • StaRs enable antibody discovery by stabilizing the GPCR protein outside the cellular membrane in the correct conformation. (centerwatch.com)
  • By using a mRNA having 5'cap and a poly(A)-tail with this extract in combination with a proprietary liposome, the translation reaction in vitro yields ample quantity of membrane protein which is captured by the liposome leading to correct conformation and folding essential for biological function. (abnova.com)
  • The NS1 structures reveal distinct domains for membrane association of the dimer and interactions with the immune system and are a basis for elucidating the molecular mechanism of NS1 function. (nih.gov)
  • The methodology will include site-directed mutagenesis, covalent protein modifications, patch- clamp measurements, thermodynamic and kinetic analysis of dose-response curves and rates of transitions, videomicroscopy, and molecular modeling. (usda.gov)
  • Following these data, in collaboration with Dr. H. R. Guy (NIH), we built molecular models for the E. coli MscL in the closed, closed-expanded and open conformations and found that the channel can not be gated solely with gate that is placed within the transmembrane domain as was previously proposed (Spencer et al. (usda.gov)
  • This valuable study reports multi-scale molecular dynamics simulations to investigate a class of highly potent antibodies that simultaneously engage with the HIV-1 Envelope trimer and the viral membrane. (elifesciences.org)
  • A proprietary reagent solution that is composed of proteins, glycoproteins, lipids and low molecular weight components that self-associate to form a complex macromolecular matrix. (invitrointl.com)
  • To achieve this goal we use a combination of x-ray crystallography, molecular modelling, molecular docking and molecular biology in collaboration with medicinal chemistry to engineer drugs that specifically interact with and disable critical bacterial target proteins. (ubc.ca)
  • 3) when the S1 bundle breaks apart leading to the fully open conformation, S1 helices may dock to a specific site on the inner surface of the pore. (usda.gov)
  • Since the method does not depend on specific resonance assignments and proves robust toward nonidealities in the sample alignment, it may be generally feasible for the study of conformational arrangement and function-induced conformation changes of large integral membrane proteins. (ox.ac.uk)
  • Antibodies can target epitopes on integral membrane proteins very near to the lipid bilayer surface, even those partially embedded within the headgroup region. (elifesciences.org)
  • Of note, domains found in membrane-attack complex proteins of complement and perforin (MACPF) resemble cholesterol-dependent cytolysins from Gram-positive bacteria, and the Bcl family of apoptosis regulators share similar architectures with Escherichia coli pore-forming colicins. (nih.gov)
  • 1979. The conformation of Escherichia coli ribosomal protein L7/Ll2 in solution: hydrodynamic, spectro¬scopic, and conformation prediction studies. (mote.org)
  • Structural Insights into Inhibition of Escherichia coli Penicillin-binding Protein 1B. (ubc.ca)
  • Sodium/substrate symport (or co-transport) is a widespread mechanism of solute transport across cytoplasmic membranes of pro- and eukaryotic cells. (embl.de)
  • In the following, we refer to pump of halophilic archaebacteria, has become the these as the extracellular (EC) channel and the paradigm of membrane proteins in general and ion cytoplasmic (CP) channel. (lu.se)
  • Experimental two-dimensional (1)H-(15)N dipole-dipole coupling versus (15)N chemical shift spectra for all samples are analyzed numerically to establish combined constraints on the orientation of the seven transmembrane helices relative to the membrane bilayer normal. (ox.ac.uk)
  • Signaling is then transduced across the membrane by altering the configuration of the transmembrane (TM) domain, by dimerization of the TM helical region or a change in the arrangement of the TM helices within such a dimer. (biorxiv.org)
  • Specifically, the TM of unliganded (inactive) EGFR dimerizes at the C-terminus (C t ), while the ligand bound form dimerizes N-terminally (N t ), and the two helices are also rotated by 180° between the conformations ( 4 , 5 ). (biorxiv.org)
  • We also propose that stretch in the membrane increases the tilt of the M1 and M2 helices, making the barrel wider. (usda.gov)
  • All GPCRs share a structural motif consisting of seven membrane-spanning helices, and exist in both active and inactive forms. (thermofisher.com)
  • The flavivirus nonstructural protein 1 (NS1) functions in genome replication as an intracellular dimer and in immune system evasion as a secreted hexamer. (nih.gov)
  • The ligand-bound TM configuration promotes asymmetric dimerization of the intracellular juxta-membrane (JM) and kinase domains, which causes autophosphorylation of intracellular tyrosine residues ( 6 , 7 ). (biorxiv.org)
  • secretase is internalized from the cell membrane into intracellular compartments (endosomes) where it cleaves its substrate, Amyloid Precursor Protein (APP). (healthnewstrack.com)
  • The work provides insights into how broadly neutralizing antibodies associate with lipids proximal to membrane-associated epitopes to drive neutralization. (elifesciences.org)
  • Conversely, propensity to bind lipids or cell membranes poses a significant auto-immunity risk. (elifesciences.org)
  • As a toxin, PLA2 is a potent presynaptic neurotoxin which blocks nerve terminals by binding to the nerve membrane and hydrolyzing stable membrane lipids. (nih.gov)
  • The most promising Ags were submitted to a more in-depth biophysical investigation of their structure and conformation stability, and for their capacity to interact with neutralising antibodies (Abs) and peptides. (europa.eu)
  • 1980. The conformational stability of ribosomal protein L7/Ll2: The effects of pH, temperature and guani¬dinium chloride. (mote.org)
  • This system has significant advantages over commonly used protein expression platforms such as E. coli , insect cell, and mammalian cell which are all in vivo systems and under the constraint of cell membrane leading to low yield and stability. (abnova.com)
  • The formation of the ternary complex induces another structural change that exposes sodium and substrate to the other site of the membrane. (embl.de)
  • Substrate and sodium are released and the empty transporter re-orientates in the membrane allowing the cycle to start again. (embl.de)
  • The transport domains are mobile and move through the lipid bilayer (alike an elevator) when translocating the amino acid substrate and co-transported ions across the membrane 13 . (nature.com)
  • This study is a proof-of-concept that acidity-responsive membrane peptide ligands can be generally applied to RTKs. (biorxiv.org)
  • An array of activating ligands participate in the conformation of GPCRs which leads to signaling via G-proteins and downstream effectors. (thermofisher.com)
  • B-cells producing antibodies targeting host membranes are downregulated in healthy organisms 1 - 4 . (elifesciences.org)
  • Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. (joplink.net)
  • Our data provide proof-of principle of a new approach for directing small molecule inhibitors to disease causing RAFT targets in cellular membranes. (healthnewstrack.com)
  • Furthermore, this assay can be readily adapted for studying protein-ligand interactions in intact mitochondria and in other cell organelles extending its implications for pesticide and pharma industry and in drug discovery. (cgiar.org)
  • We propose that the resulting disruption of native TM interactions disrupts the conformation of the kinase domain, inhibiting EGFR's ability to send migratory cell signals. (biorxiv.org)
  • The study of MscL, a highly convenient model system, will give us basic understanding and the first example of what type of intramolecular interactions, sequence motifs, and conformations make membrane proteins sensitive to a physiologically relevant mechanical stimulation. (usda.gov)
  • All-atom simulations of 4E10, PGZL1, 10E8 and LN01 docked onto HIV-like membranes consistently form phospholipid complexes at key complementarity-determining region loop sites, solidifying that stable and specific lipid interactions anchor bnAbs to membrane surfaces. (elifesciences.org)
  • Then, evolving tolerance or even affinity to lipid bilayers could be beneficial in recognition of membrane-proximal epitopes, for example gaining avidity and specificity via cooperative interactions simultaneously with the epitope and membrane. (elifesciences.org)
  • Ribosome interactions anchor the Sec61/TRAP complex in a conformation that renders. (lu.se)
  • Ribosome interactions anchor the Sec61/TRAP complex in a conformation that renders the ER membrane locally thinner by significantly curving its lumenal leaflet. (lu.se)
  • In order to deepen our understanding of the interactions between the cell membrane and nanostructures, we perform STED microscopy live imaging of the membrane of cells cultured on nanostructures. (lu.se)
  • Hatzis P, Kyrmizi I, Talianidis I. (2006) Mitogen-activated protein kinase-mediated disruption of enhancer-promoter communication inhibits hepatocyte nuclear factor 4alpha expression. (forth.gr)
  • The corneal irritancy of test materials is known to be related to their propensity to promote denaturation and disruption of corneal proteins. (invitrointl.com)
  • Neurons can release small molecule neurotransmitters very rapidly in part because synaptic vesicles are docked to the membrane at active zones. (rupress.org)
  • The absence of glucose, Na + , a membrane potential in vesicles, or the directionality of water flow did not grossly altered the pf. (tcdb.org)
  • Influx of calcium ions into the presynaptic nerve terminal causes vesicles (loaded with neurotransmitters) migrate toward the presynaptic membrane. (bloodraynebetrayal.com)
  • Neurotransmitters are released from the axon terminal when their vesicles "fuse" with the membrane of the axon terminal, spilling the neurotransmitter into the synaptic cleft. (bloodraynebetrayal.com)
  • We also investigate the properties of model membranes such as supported lipid bilayer and vesicles on a variety of nanowire substrates. (lu.se)
  • During chemiosmosis, the free energy from the series of reactions that make up the electron transport chain is used to pump hydrogen ions across the membrane, establishing an electrochemical gradient. (roadlesstraveledstore.com)
  • The β-sheet conformation causes negative Gaussian curvature and membrane dehydration, which are required for membrane merger, while the α-helical conformation resides in low-curvature lamellar membranes and forms three-helix bundles. (lu.se)
  • Another design strategy was developed using gammaretroviruses backbone where 2 domains were substituted with HIV epitopes in the N-terminal part of the ectodomain and the membrane proximal external region (MPER) respectively. (europa.eu)
  • The pairs of residues on S1 and S2 predicted to be proximal in either closed or open conformations were mutated to Cys, and the effects of coupling were tested with patch-clamp and biochemically using Western blots. (usda.gov)
  • A potent class of HIV-1 broadly neutralizing antibodies (bnAbs) targets the envelope glycoprotein's membrane proximal exposed region (MPER) through a proposed mechanism where hypervariable loops embed into lipid bilayers and engage headgroup moieties alongside the epitope. (elifesciences.org)
  • A better understanding of how antibodies develop membrane affinity and target membrane-proximal epitopes would be impactful for antibody therapeutics, auto-immunity, and vaccine development 10 - 12 . (elifesciences.org)
  • We sought to address this phenomenon for broadly neutralizing antibodies (bnAbs) 4E10, PGZL1, 10E8, and LN01 of unique lineages which all target the semi-concealed membrane-proximal epitope region (MPER) 13 - 16 of the HIV-1 envelope glycoprotein (Env). (elifesciences.org)
  • 2) For the HIV fusion protein gp41, we have determined the oligomeric structure of the membrane-proximal external region (MPER) and the TMD. (lu.se)
  • These and other correlations provide considerable help in understanding the structural requirements for membrane binding and pore formation. (nih.gov)
  • MscL, a mechanosensitive channel of large conductance, was the first isolated molecule shown to respond to membrane stretch by opening a large aqueous pore. (usda.gov)
  • 1998, Science 282:2220-2226) provided a strong framework for the evaluation of conformations that permit opening of a large MscL pore by membrane tension. (usda.gov)
  • After binding to target membranes, the protein assembles into a pre-pore complex. (joplink.net)
  • A conformation change leads to insertion in the host membrane and formation of an oligomeric pore complex. (joplink.net)
  • Cholesterol may be required for binding to host cell membranes, membrane insertion and pore formation. (joplink.net)
  • Heptares' StaRs offer a breakthrough solution to the main challenge associated with making antibodies against GPCRs, namely producing purified and properly folded protein for use as an antigen against which antibodies are raised. (centerwatch.com)
  • This has previously proved very difficult for GPCRs, which lose their conformational and functional integrity when removed from their natural environment in cell membranes. (centerwatch.com)
  • GPCRs are expressed in every type of cell in the body where their function is to transmit signals from outside the cell across the membrane to signaling pathways within the cell, between cells and between organ systems. (centerwatch.com)
  • The analysis of mutations now allows us to narrow the search for functionally important regions to the short N-terminal helix, two transmembrane domains of the protein, and the loop between them. (usda.gov)
  • The average hydropathy plot for SSSF proteins predicts 11 to 15 putative transmembrane domains (TMs) in alpha-helical conformation. (embl.de)
  • The Abeta plaques are a pathological hallmark of Alzheimer's disease, Abeta is the first biomarker that is altered, heritable forms of Alzheimer's rely on mutations in the Abeta protein* or enzymes involved in its production, and a mutation that reduces Abeta production protects against AD. (lu.se)
  • 1992. Interaction of antimicrobial dermaseptin and its fluorescently labeled analogs with phospholipid membranes. (was.org)
  • The modeling platform developed here uncovers insights into lipid participation in antibodies' recognition of membrane proteins and highlights antibody features to prioritize in vaccine design. (elifesciences.org)
  • Secretins revealed: structural insights into the giant gated outer membrane portals of bacteria. (ubc.ca)
  • I will present our recent structural studies, using solid-state NMR spectroscopy, of three membrane proteins that give insights into the mechanisms of virus-cell membrane fusion and virus budding. (lu.se)
  • Both the CAPS and UNC-13 docking pathways converge on syntaxin, a component of the SNARE (soluble N -ethyl-maleimide-sensitive fusion protein attachment receptor) complex. (rupress.org)
  • by similarity, to similar proteins with structure having catalytic activity. (nih.gov)
  • Application of an irritant chemical to the membrane disc disrupts the ordered structure of keratin and collagen and results in release of the bound indicator dye. (invitrointl.com)
  • Within these terminals, alpha-synuclein interacts with phospholipids and proteins. (wikipedia.org)
  • 2006) Senseless physically interacts with proneural proteins and functions as a transcriptional co-activator. (forth.gr)
  • To understand how cholesterol interacts with M2 to generate membrane curvature, we have determined the cholesterol-binding site of M2 by measuring protein-cholesterol distances and cholesterol orientation in the membrane. (lu.se)
  • Changes in emission spectra of tryptophan are due to the protein conformational transitions, subunit association, ligand binding or denaturation, which affect the local environment surrounding the indole ring. (cgiar.org)
  • Fifty-one vaccine candidates were designed mainly to mimic fusion intermediate conformations of gp41 and in some instance to increase exposure of the 2F5/4E10 epitopes. (europa.eu)
  • Coarse-grained simulations effectively capture antibodies embedding into membranes. (elifesciences.org)
  • Simulations estimating protein-membrane interaction strength for PGZL1 variants along an inferred maturation pathway show bilayer affinity is evolved and correlates with neutralization potency. (elifesciences.org)
  • Crystal structures often deviate from the natural system: Proteins, especially membrane-bound ones, do not necessarily crystallize in their biologically active structures and the measures needed to obtain suitable GPCR crystals tend to increase the diversity between the natural environment and the crystal. (gauss-centre.eu)
  • amyloid peptide, can be inhibited effectively using compounds anchored to cell membrane RAFTS. (healthnewstrack.com)
  • 1997. Membrane permeabilization mechanisms of a cyclic antimicrobial peptide, tachyplesin I, and its linear analog. (was.org)
  • For the PIV5 fusion protein, the fusion peptide (FP) and the transmembrane domain (TMD) show striking membrane-dependent conformations. (lu.se)
  • Some of you may be annoyed by the fact I call Abeta a protein, and yes, indeed, it is a peptide, but let's just simplify it, calling it a protein for now. (lu.se)
  • M. Tarek : On the structure dynamics and electrical properties of membranes hydration water. (cea.fr)
  • A membrane receptor reagent and assay is disclosed in which liposomes are bound to an evanescent wave emitting surface. (google.com)
  • Controlled mixing of the test material and the reagent solution during the assay incubation period promotes protein denaturation and disaggregation of the macromolecular matrix. (invitrointl.com)
  • The changes in protein structure that are induced by the test material may be readily quantitated by measuring the resulting changes in turbidity (OD405) of the reagent solution. (invitrointl.com)
  • A reagent solution consisting of a highly organized globulin/protein macromolecular matrix. (invitrointl.com)
  • Additionally, dermal irritants induce changes in conformation in the globular proteins found in the reagent solution. (invitrointl.com)
  • The extent of dye release and protein denaturation may be quantitated by measuring the changes in optical density of the reagent solution at 450 nm (OD450). (invitrointl.com)
  • An alpha-synuclein fragment, known as the non-amyloid beta (non-abeta) component (NAC) of Alzheimer's disease amyloid, originally found in an amyloid-enriched fraction, was shown to be a fragment of its precursor protein, NACP. (wikipedia.org)
  • The Enterobacteriaceae, which are quite common in the human gut, can create curli, which are functional amyloid proteins. (wikipedia.org)
  • Oral injection of curli-producing bacteria can also boost formation and aggregation of the amyloid protein Syn in old rats and nematodes. (wikipedia.org)
  • Conformation and topology of amyloid beta-protein adsorbed on a tethered artificial membrane probed by surface plasmon field-enhanced fluorescence spectroscopy. (mpg.de)
  • The aggregation of the protein Amyloid-beta (Abeta) into plaques outside the nerve cells has been recognized in patients with Alzheimer's disease since 1905. (lu.se)
  • The overall objective of EuroNeut-41 was to design a vaccine that raises antibodies able to prevent HIV cell fusion by blocking the virus fusion protein gp41 in its pre-fusogenic conformation. (europa.eu)
  • The electrons cause conformation changes in the shapes of the proteins to pump H+ across a selectively permeable cell membrane. (roadlesstraveledstore.com)
  • 1978. Altered erythrocyte membrane proteins in sickle cell patients associated with the severity of the disease. (mote.org)
  • JADO Technologies GmbH, the leading developer of RAFT intervention therapeutics, announced the publication of a paper in Science demonstrating a potential novel strategy for therapeutic intervention in Alzheimer's and other diseases by targeting discrete sub-compartments in the cell membrane (RAFTS). (healthnewstrack.com)
  • The protein then switches to an active form when DNA damage is detected to trigger the process of cell self-destruction. (elifesciences.org)
  • If the cell contains proteins, which cannot pass through the cell membrane and there is potassium chloride, which can pass through the cell membrane, the product of potassium and chloride concentrations in the cell will be equal to the product of potassium and chloride concentrations on the outside at equilibrium. (iupac.org)
  • Furthermore, there are the proteins in the cell, which also adds to the osmotic pressure. (iupac.org)
  • In the following years it was shown that cell membrane permeability to sodium was a general phenomena. (iupac.org)
  • By then the existence of the ion pump in the cell membrane had been accepted, but it was not known what the nature of the pump was. (iupac.org)
  • Therefore, the local lipid composition of the membrane is a key regulator of the site of virus-cell fusion. (lu.se)
  • Synuclein is however found predominantly in the presynaptic termini, in both free or membrane-bound forms, with roughly 15% of synuclein being membrane-bound at any moment in neurons. (wikipedia.org)
  • The detection and evaluation of concentration of influenza virus proteins in biological samples is critical in a broad range of medical and biological investigations regarding the concern over potential outbreaks of virulent influenza strains in animals and humans. (omicsonline.org)
  • Protons flow down their concentration gradient into the matrix through the membrane protein ATP synthase, causing it to spin (like a water wheel) and catalyze conversion of ADP to ATP. (roadlesstraveledstore.com)
  • But as there must be electro-neutrality on the inside, the concentration of potassium must be higher than the chloride concentration, because part of the potassium concentration is used to neutralize the protein negative charges. (iupac.org)
  • The data represent the first direct determination of the cholesterol-binding structure of a membrane protein in lipid bilayers, and moreover indicate a specific mechanism by which cholesterol concentration gradients in the membrane drive the M2 protein to the neck of the budding virus to conduct membrane scission. (lu.se)
  • This membrane interaction behavior is attributed to shared CDR loop features, including a long hydrophobic CDR- H3 7 , 18 , 21 , 22 , 24 - 26 , and appears to correlate with neutralization potency. (elifesciences.org)
  • Shortly after SARS-CoV emerged at the turn of the 21st century, the spike (S) protein (particularly in its native conformation) was identified as the immunodominant antigen of the virus3. (who.int)
  • Effector proteins are then recruited to activate various cellular signaling pathways, including RAS/RAF/MEK, PI3K/AKT/mTOR, and JAK/STAT ( 1 ). (biorxiv.org)
  • Protein translocation across the endoplasmic reticulum (ER) membrane is an essential step during protein entry into the secretory pathway. (lu.se)
  • The conserved Sec61 protein-conducting channel facilitates polypeptide translocation and coordinates cotranslational polypeptide-processing events. (lu.se)
  • In cells, the majority of Sec61 is stably associated with a heterotetrameric membrane protein complex, the translocon-associated protein complex (TRAP), yet the mechanism by which TRAP assists in polypeptide translocation remains unknown. (lu.se)
  • From the MRD data, the rotational correlation time of detergent-solubilized BR was determined to 35 ns at 300 K, con- sistent with a monomeric protein in complex with about 150 detergent molecules. (lu.se)
  • While the locations and inferred H-bond connec- water molecules in a membrane protein, despite tivities of these internal water molecules are essen- being deeply buried and extensively H-bonded to tial, one would also like to quantify their mobility. (lu.se)
  • Later, when Abeta also accumulates in critical amounts outside the cells, this may suck out the internal Abeta, like osmosis of molecules over a membrane to have equal concentrations on both sides. (lu.se)
  • Studying the role of arginine residues in enhancing an antifreeze protein from the beetle Dendroides candensis , Brown hopes to understand how the protein works as an adaptive mechanism for many organisms in cold environmental conditions. (calstatela.edu)
  • Alpha-synuclein is a synuclein protein of unknown function primarily found in neural tissue, making up as much as one percent of all proteins in the cytosol of brain cells. (wikipedia.org)
  • Membrane curvature generation and membrane remodeling underlie many biological processes such as virus entry into cells and virus budding. (lu.se)
  • Abnova in vitro protein expression system is based on the eukaryotic translational apparatus of wheat germ. (abnova.com)
  • Antifreeze proteins allow plants, animals and bacteria to survive in sub-zero environments by stopping ice crystals from forming. (calstatela.edu)
  • We find that this domain is trimerized in the lipid membrane with a helix-turn-helix conformation, suggesting that this domain stabilizes the trimer structure of gp41 and promotes membrane curvature during the fusion process. (lu.se)
  • Alpha-synuclein is a neuronal protein that regulates synaptic vesicle trafficking and subsequent neurotransmitter release. (wikipedia.org)
  • We have found that the calcium-activated protein for secretion (CAPS) protein is required for dense core vesicle docking but not synaptic vesicle docking. (rupress.org)
  • CAPS function in dense core vesicle docking parallels UNC-13 in synaptic vesicle docking, which suggests that these related proteins act similarly to promote docking of independent vesicle populations. (rupress.org)
  • Then, the vesicle and membrane fuse, and neurotransmitter is released into the synaptic cleft by exocytosis. (bloodraynebetrayal.com)
  • secretase inhibitor to the membrane would confer inhibitory activity upon it by bringing it to the right compartment. (healthnewstrack.com)