• Macropinocytosis GTPase Rab Phosphatase Phosphoinositide EGF Ruffling Macropinocytosis is an actin-driven process that involves the formation and extension of plasma membrane ruffles and the eventual closure of large (≥0.2 to 5.0. (biologists.com)
  • The proton motive force is maintained by export of protons by a plasma membrane H + -ATPase, and therefore this sugar transport system requires net investment of ATP for its activity [ 20 ]. (biomedcentral.com)
  • Taken together, these findings provide strong evidence that the generation of PtdIns(4)P is sufficient to trigger forward transport from the Golgi to the plasma membrane and that Sac1p is critically required for the termination of this signal. (embl.de)
  • Yamazaki Y and Kono K (2022) Clathrin-mediated trafficking of phospholipid flippases is required for local plasma membrane/cell wall damage repair in budding yeast. (yeastgenome.org)
  • A novel membrane protein, Ros3p, is required for phospholipid translocation across the plasma membrane in Saccharomyces cerevisiae. (yeastgenome.org)
  • Plasma membrane polarization during mating in yeast cells. (ox.ac.uk)
  • Endocytic recycling and slow diffusion on the plasma membrane were shown to facilitate polarized surface distribution of Snc1p (Valdez-Taubas, J., and H.R. Pelham. (ox.ac.uk)
  • Furthermore, we provide evidence that the lipid bilayer at the mating projection is more condensed than the plasma membrane enclosing the cell body, and that sphingolipids are required for this lipid organization. (ox.ac.uk)
  • EN] The plasma membrane contains a wide spectrum of transporter proteins, each responsible for a facet of the cell's nutritional and signalling requirements. (upv.es)
  • The di-lysine motif of Emp47p was functional when transplanted onto Ste2p, a plasma membrane protein, conferring ER localization. (rupress.org)
  • It mediates fusion of synaptic vesicles with the presynaptic plasma membrane resulting in exocytosis of neurotransmitters. (researchgate.net)
  • The Hansenula polymorpha PER9 gene encodes a peroxisomal membrane protein essential for peroxisome assembly and integrity. (microbiologyresearch.org)
  • chb-3 encodes a novel protein, with a zf-MYND motif and ankyrin repeats, that is highly conserved from worm to human. (stanford.edu)
  • The Saccharomyces cerevisiae EMP47 gene encodes a nonessential type-I transmembrane protein with sequence homology to a class of intracellular lectins defined by ERGIC-53 and VIP36. (rupress.org)
  • Among CRE1 repressed transcripts, genes encoding unknown proteins and transport proteins were overrepresented. (biomedcentral.com)
  • The latter, Stagljar said, is an important area for drug development, because it opens the possibility that you could block ABC transporter activity by blocking associated zinc transport proteins. (genomeweb.com)
  • S. cerevisiae is an attractive model organism due to the fact that its genome has been sequenced, its genetics are easily manipulated, and it is very easy to maintain in the lab. (jove.com)
  • Now that we've learned a bit about S. cerevisiae as an organism, let's discuss what makes it a great model system for research. (jove.com)
  • In this study we expressed plant DCL4 in Saccharomyces cerevisiae , an RNAi-depleted organism, in which we could highlight the role of dicing as neither Argonautes nor RNA-dependent RNA polymerase is present. (biomedcentral.com)
  • Cell fractionation analysis as well as studies using a yeast strain that is conditionally deficient in glycosylation demonstrate that TIP1 is a heavily modified membrane‐associated protein. (researchwithrutgers.com)
  • In this study, we show the use of direct external electrical stimulation of a jellyfish luminescent calcium-activated protein, aequorin, expressed in a transgenic yeast strain. (upv.es)
  • 2006. The peroxisomal membrane protein Inp2p is the peroxisome-specific receptor for the myosin V motor Myo2p of Saccharomyces cerevisiae. (reginamaria.ro)
  • Proteins targeted to the peroxisome (in plants called glyoxisomes) are synthesized in the cytoplasm of the cell and are targeted to the peroxisome post-translationally, possibly via multiple pathways. (tcdb.org)
  • 2023 ). Imaging fluorescence cross-correlation spectroscopy showed that cargo import correlates with transient focusing of GFP-Pex13 and GFP-Pex14 on the peroxisome membrane. (tcdb.org)
  • This subcomplex also exists in the peroxisome membrane. (tcdb.org)
  • C.J. Slubowski, S.M. Paulissen, and L.S. Huang (2014) The GCKIII kinase Sps1 and the 14-3-3 isoforms, Bmh1 and Bmh2, cooperate to ensure proper sporulation in Saccharomyces cerevisiae. (umb.edu)
  • L.S. Huang, H. K. Doherty, and I. Herskowitz (2005) The Smk1p MAP kinase negatively regulates Gsc2p, a 1, 3-beta-glucan synthase, during spore wall morphogenesis in Saccharomyces cerevisiae. (umb.edu)
  • Through biochemical purification of the MAP kinase Smk1, her lab identified several proteins that physically interact with Smk1, including Gsc2, a subunit of beta-glucan synthase, which plays an important role in spore wall synthesis. (umb.edu)
  • Patrick A. Lewis ABSTRACT Leucine-rich repeat kinase 2 (LRRK2) is a multidomain scaffolding protein with dual guanosine triphosphatase ( GTPase ) and kinase enzymatic activities, providing this protein with the capacity to regulate a multitude of signalling pathways. (biologists.com)
  • Previous analyses suggest that lack of normal cilia causes the small-body phenotype through the activation of a signaling pathway which consists of the EGL-4 cGMP-dependent protein kinase and the GCY-12 receptor-type guanylyl cyclase. (stanford.edu)
  • His studies in Transport protein integrate themes in fields like Glycerol-3-phosphate dehydrogenase, NAD+ kinase, Membrane transport, Genome and Membrane protein. (research.com)
  • His research in Mitochondrial carrier intersects with topics in Membrane transport, ATP-ADP translocase, NAD+ kinase and Solute carrier family. (research.com)
  • His Receptor Protein-Tyrosine Kinases research extends to the thematically linked field of Receptor tyrosine kinase. (research.com)
  • Receptor Protein-Tyrosine Kinases is closely attributed to Tyrosine kinase in his research. (research.com)
  • The SAD1/RAD53 protein kinase controls multiple checkpoints and DNA damage-induced transcription in yeast. (academicinfluence.com)
  • They are members of the rBAT family of mammalian proteins (TC #8.A.9). (tcdb.org)
  • Researchers from the University of Toronto have created a map of the protein interactions of 19 members of the ATP-binding cassette transporter class in a species of yeast, combining this data with previously-reported ABC transporter interactions into a comprehensive "interactome. (genomeweb.com)
  • Though the group did the mapping with ABC transporters from a yeast species - Saccharomyces cerevisiae - many of the proteins are homologous to disease-linked human ABC proteins. (genomeweb.com)
  • The simplest form of RNA silencing, widely known as RNA interference (RNAi), is conserved in diverse eukaryotic species, including the fungal kingdom, but has been lost in the model budding yeast, Saccharomyces cerevisiae [ 15 ]. (biomedcentral.com)
  • In this review we want to give an introduction to the functional role played by ubiquitination during peroxisomal protein import and highlight the mechanistic concepts that have emerged based on data derived from different species since the discovery of the first ubiquitinated peroxin 15years ago. (ox.ac.uk)
  • Its genome, which is approximately 14.1 million base pairs, is estimated to contain 4,970 protein-coding genes and at least 450 non-coding RNAs. (wikipedia.org)
  • S. cerevisiae has ∼ 6,000 genes, so measuring all pairwise PPIs in one environment requires ∼ 18 million strains to be assayed, each of which characterizes a single PPI. (nature.com)
  • Over two dozen proteins involved in protein import and membrane insertion, peroxins, encoded by PEX genes, have been characterized. (tcdb.org)
  • EN] Mot3 and Rox1 are transcriptional repressors of hypoxic genes. (upv.es)
  • Genes that encode the proteins of complement components or their isotypes are distributed throughout different chromosomes, with 19 genes comprising 3 significant complement gene clusters in the human genome. (medscape.com)
  • V-ATPase is regulated by a unique mechanism that involves reversible dissociation into V₁-ATPase and V₀ proton channel, a process that involves breaking of protein interactions mediated by subunit C, the cytoplasmic domain of subunit "a" and three "peripheral stalks," each made of a heterodimer of E and G subunits. (rcsb.org)
  • Several large-scale efforts have systematically catalogued protein-protein interactions (PPIs) of a cell in a single environment. (nature.com)
  • Patrick JW, Laganowsky A. Probing Heterogeneous Lipid Interactions with Membrane Proteins Using Mass Spectrometry. (avantilipids.com)
  • The combination of such self-organizational phenomena with canonical intermolecular interactions is most likely to control the release of membrane proteins from the ER into the secretory pathway. (biologists.com)
  • This process depends on the general physico-chemical features of the cargo membrane protein and on the interactions of these features with the collective properties of the bilayer, instead of the one-to-one intermolecular interactions that exist between discrete signals and their receptors. (biologists.com)
  • The Sec1p / Munc18 (SM) family are a conserved group of proteins that regulate membrane fusion through interactions with their cognate syntaxins. (gla.ac.uk)
  • Finally, I have developed an in vitro fusion assay to enable us to dissect the functional significance of the various interactions that Vps45p displays with its cognate SNARE proteins. (gla.ac.uk)
  • Overall, membrane protein interactions are difficult to study because of their biochemical features," he said. (genomeweb.com)
  • Stagljar and his colleagues previously developed a method, called a membrane yeast two-hybrid, or MYTH assay to allow them to study interactions of full-length membrane proteins in their natural cellular environment. (genomeweb.com)
  • Briefly, MYTH adapts a previously developed split ubiquitin method as an in vivo sensor of protein-protein interactions. (genomeweb.com)
  • Overall, the study yielded a map covering 537 unique binary interactions across 366 proteins, the study authors wrote, which they annotated with functional classifications to create a standard map, as well as a map showing protein conservation in humans and known disease associations. (genomeweb.com)
  • The largest group - about 26 percent of the interactions - corresponds to proteins involved in transport and related processes. (genomeweb.com)
  • The second largest, with 16 percent, marks interactions with proteins of unknown function. (genomeweb.com)
  • Together, these suggest the dynamic nature of interactions within the subunits of the PAM complex, as well as between TOM and TIM23 subunits, during the process of translocation of a protein into mitochondria. (uni-goettingen.de)
  • Together, these results expand our knowledge about the molecular interactions between mitochondrial translocase components during active protein import, as well as increase our repertoire of the TIM22 complex substrates. (uni-goettingen.de)
  • Saccharomyces cerevisiae (commonly known as baker's yeast) is a single-celled eukaryote that is frequently used in scientific research. (jove.com)
  • Saccharomyces cerevisiae , otherwise known as baker's yeast, is one of the many model organisms studied in laboratories all over the world. (jove.com)
  • Mitochondrial protein import in trypanosomatids: Variations on a theme or fundamentally different? (nih.gov)
  • Ferdinando Palmieri mainly investigates Biochemistry, Mitochondrion, Mitochondrial carrier, Transport protein and Inner mitochondrial membrane. (research.com)
  • His Biochemistry research focuses on Gene isoform, Yeast, Mitochondrial matrix, Gene and Membrane transport protein. (research.com)
  • His Mitochondrial carrier research integrates issues from Mitochondrial membrane transport protein, ATP-ADP translocase, Saccharomyces cerevisiae and Citrate synthase. (research.com)
  • His Inner mitochondrial membrane research incorporates elements of Phosphate and Citric acid cycle. (research.com)
  • His main research concerns Biochemistry, Mitochondrion, Mitochondrial carrier, Inner mitochondrial membrane and Molecular biology. (research.com)
  • Ferdinando Palmieri spends much of his time researching Biochemistry, Mitochondrial carrier, Mitochondrion, Inner mitochondrial membrane and Cell biology. (research.com)
  • His work is connected to Membrane transport, Saccharomyces cerevisiae, Carnitine, Mitochondrial transport and Mitochondrial matrix, as a part of Biochemistry. (research.com)
  • His work deals with themes such as Amino acid, Transport protein, Nucleotide, Gene and Stereochemistry, which intersect with Inner mitochondrial membrane. (research.com)
  • His primary areas of study are Biochemistry, Mitochondrial carrier, Mitochondrion, Inner mitochondrial membrane and Cell biology. (research.com)
  • The TIM23 and the TIM22 complexes in the inner mitochondrial membrane are essential transport systems for proper insertion of inner membrane and matrix proteins in mitochondria. (uni-goettingen.de)
  • Mpc proteins are highly conserved from yeast to humans and are necessary for the uptake of pyruvate at the inner mitochondrial membrane, which is used for leucine and valine biosynthesis and as a fuel for respiration. (upv.es)
  • This process results in the creation of a proton gradient over the inner mitochondrial membrane, which drives the ATP synthesis. (lu.se)
  • These proteins are often homologous, and their similar sequences indicate that the organisms share a common ancestor. (jove.com)
  • Cdc50p, a protein required for polarized growth, associates with the Drs2p P-type ATPase implicated in phospholipid translocation in Saccharomyces cerevisiae. (yeastgenome.org)
  • 2011) addresses two of these aspects, the translocation of soluble proteins into the peroxisomal matrix and the biogenesis of the peroxisomal membrane. (tcdb.org)
  • The Sec61 heterotrimer forms an evolutionarily conserved channel for translocation of secreted proteins and integration of membrane proteins. (umassmed.edu)
  • We are using the Saccharomyces cerevisiae experimental to analyze the mechanism of translocation channel gating by ribosome-nascent chain complexes. (umassmed.edu)
  • The in vivo kinetics of protein translocation channel gating are being analyzed using ubiquitin translocation assay (UTA) reporters. (umassmed.edu)
  • Translocation of proteins through the Sec61 and SecYEG channels. (umassmed.edu)
  • Most peroxisomal membrane proteins do not have PTS1 or PTS2, and insertion requires several peroxins distinct from those required for import of soluble matrix proteins. (tcdb.org)
  • The functionality of peroxisomes depends on the proper import of their matrix proteins. (ox.ac.uk)
  • Peroxisomal matrix proteins are imported posttranslationally in a folded, sometimes even oligomeric state. (ox.ac.uk)
  • Taken together, these results demonstrate that curcumin is a PPARα activator and may affect expression levels of proteins involved in amyloid deposition to influence amyloidosis and metabolism in a complex manner. (elifesciences.org)
  • Here, we found that polarization of Fus1p, a raft-associated type I transmembrane protein involved in cell fusion, does not depend on endocytosis. (ox.ac.uk)
  • Some animal proteins, for example, those in the LAT family (TC# 2.A.3.8) including ASUR4 (gbY12716) and SPRM1 (gbL25068) associate with a type 1 transmembrane glycoprotein that is essential for insertion or activity of the permease and forms a disulfide bridge with it. (tcdb.org)
  • A large pore is formed by transmembrane proteins. (tcdb.org)
  • For membrane proteins, a third mechanism, based on the interaction of their transmembrane domain (TMD) with lipid microdomains, must also be considered. (biologists.com)
  • These proteins have predominantly been defined to contain either four or six transmembrane domains. (uni-goettingen.de)
  • The peroxisomal protein import machinery, which shares similarities with chloroplasts, is unique in transporting folded and large (up to 10 nm in diameter) protein complexes into peroxisomes. (tcdb.org)
  • It won't work if you want to co-immunoprecipitate them or apply any proteomic biochemical method because you will have to use detergents, and then you destroy all the protein complexes. (genomeweb.com)
  • Additionally, both complexes recognise different targeting signals on a precursor protein. (uni-goettingen.de)
  • Biochemical experiments have revealed that the two SST3 proteins (STT3A and STT3B) assemble with a shared set of non-catalytic subunits into two separate OST complexes with different kinetic properties. (umassmed.edu)
  • New technologies for purifying membrane-bound protein complexes in combination with cryo-electron microscopy (EM) have recently allowed the exploration of such complexes under near-native conditions. (researchgate.net)
  • In yeast (Saccharomyces cerevisiae), complex III and complex IV are respiratory chain complexes capable of transferring electrons to oxygen converting it to water. (lu.se)
  • Because many yeast proteins are similar in sequence and function to those found in other organisms, studies performed in yeast can help us to determine how a particular gene or protein functions in higher eukaryotes (including humans). (jove.com)
  • By investigating the function of a given protein in yeast, researchers gain insight into the protein's function in higher eukaryotes, such as us, humans. (jove.com)
  • In this Commentary, I review evidence in favor of the idea that partitioning of TMDs into bilayer domains that are endowed with distinct physico-chemical properties plays a pivotal role in the transport of membrane proteins within the early secretory pathway. (biologists.com)
  • One half of a split ubiquitin molecule is attached to an ABC transporter (or another cellular protein of interest) and the other half to its potential interaction targets. (genomeweb.com)
  • C.J. Slubowski, A.D. Funk, J.M. Roesner, S.M. Paulissen, and L.S. Huang (2015) Plasmids for C-terminal tagging in Saccharomyces cerevisiae that contain improved GFP proteins, Envy and Ivy. (umb.edu)
  • In the budding yeast Saccharomyces cerevisiae , the minimal transport machinery includes the membrane proteins Pex13 and Pex14 and the cargo-protein-binding transport receptor, Pex5. (tcdb.org)
  • The ATP-dependent dislocation of the PTS1 receptor from the peroxisomal membrane into the cytosol is mediated by the AAA peroxins Pex1p and Pex6p ( Platta et al . (tcdb.org)
  • The signal recognition particle (SRP), a ribonucleoprotein that binds to the polypeptide exit site on the ribosome, and the membrane bound SRP receptor (SR) function together to selectively attach a ribosome synthesizing a protein with an RER signal sequences to the Sec61 complex. (umassmed.edu)
  • This gene provides instructions for making a protein called the lamin B receptor. (medlineplus.gov)
  • During cholesterol synthesis, the sterol reductase function of the lamin B receptor allows the protein to perform one of several steps that convert a molecule called lanosterol to cholesterol. (medlineplus.gov)
  • Silve S, Dupuy PH, Ferrara P, Loison G. Human lamin B receptor exhibits sterol C14-reductase activity in Saccharomyces cerevisiae. (medlineplus.gov)
  • The important components of this system are various cell membrane-associated proteins such as complement receptor 1 (CR1), complement receptor 2 (CR2), and decay accelerating factor (DAF). (medscape.com)
  • The recruitment of specific cytosolic proteins to intracellular membranes through binding phosphorylated derivatives of phosphatidylinositol (PtdIns) controls such processes as endocytosis, regulated exocytosis, cytoskeletal organization, and cell signaling. (embl.de)
  • Identifying permissible limits of intracellular parameters such as protein expression provides important information for examining robustness. (biomedcentral.com)
  • Pex4p is a membrane protein that may facilitate cycling of Pex5p back to the cytosol. (tcdb.org)
  • The Mitochondrion study combines topics in areas such as Chromatography, Membrane transport, Liposome and Cytosol. (research.com)
  • Yeast belong to the domain Eukaryota, which is comprised of organisms with membrane-bound nuclei, referred to as eukaryotes. (jove.com)
  • Interestingly, many proteins found in yeast share similar sequences with proteins from their fellow Eukaryotes. (jove.com)
  • The oligosaccharyltransferase is a hetero-octameric integral membrane protein in higher eukaryotes. (umassmed.edu)
  • In 2006, sub-cellular localization of almost all the proteins in S. pombe was published using green fluorescent protein as a molecular tag. (wikipedia.org)
  • Current work in the lab focuses on how these morphogenic events are regulated by specific proteins in the cell. (umb.edu)
  • The Huang lab is currently investigating how Gsc2 and other Smk1p-interacting proteins contribute to Smk1's ability to coordinate cell morphogenesis. (umb.edu)
  • To facilitate uptake and conversion of glucose at low extracellular concentrations, activity of high-affinity transporters ( K M ~ 1 mM) is required, in combination with hexokinase activity to 'trap' the sugar inside the cell in its phosphorylated form and therefore maintain a glucose concentration gradient over the cellular membrane [ 15 ]. (biomedcentral.com)
  • These glycoproteins include the CD98 heavy chain protein of Mus musculus (gbU25708) and the orthologous 4F2 cell surface antigen heavy chain of Homo sapiens (spP08195). (tcdb.org)
  • Previous studies performed in S. cerevisiae that have contributed to our understanding of important cellular processes such as the cell cycle, aging, and cell death are also discussed. (jove.com)
  • Along with mushrooms and molds, S. cerevisiae belongs to the Kingdom Fungi due to the presence of a cell wall made out of chitin, a polysaccharide polymer that's found not only in Fungi, but also in the exoskeletons of insects and crustaceans. (jove.com)
  • On the other hand, S. cerevisiae undergoes cell division through a process called budding. (jove.com)
  • The objective of research in our laboratory is to understand how proteins reach their final destinations within a cell. (umassmed.edu)
  • Consistent with this finding, the Ste2p-Emp47p hybrid protein was mislocalized to the cell surface in the alpha-COP mutant, ret1-1. (rupress.org)
  • Cholesterol is an important component of cell membranes and the protective substance covering nerve cells ( myelin ). (medlineplus.gov)
  • This study provides a proof-of-concept for the replacement of the endogenous hexose transporters of S. cerevisiae by hexose-proton symport, and the concomitant decrease in ATP yield, to greatly improve the anaerobic yield of ethanol on sugar. (biomedcentral.com)
  • Plant Dicer-like proteins (DCLs) are RNase III, double-stranded RNA (dsRNA)-specific endonucleases with specialized functions in producing short (s)RNAs of 21- to 24-nucleotides (nt), including micro (mi)RNAs and short interfering (si)RNAs of endogenous or viral origin. (biomedcentral.com)
  • Despite the detailed in vitro characterization of the enzymatic properties of yeast Sac1p, the exact cellular function of this protein has remained obscure. (embl.de)
  • If the target protein expression reaches the upper limit, i.e., essential cellular functions come to a halt when the level of protein exceeds a certain limit, the gene/plasmid copy number must decrease to below the upper limit. (biomedcentral.com)
  • Each of these proteins must have a minimal requirement level (i.e., the lower limit) to support cellular growth under each of these conditions. (biomedcentral.com)
  • The MAC exerts powerful killing activity by creating perforations in cellular membranes. (medscape.com)
  • S. pombe can degrade L-malic acid, one of the dominant organic acids in wine, which makes them diverse among other Saccharomyces strains. (wikipedia.org)
  • Observing how the protein interactome changes across perturbations would require re-assaying all ∼ 18 million strains for each perturbation studied. (nature.com)
  • Regulation of peroxisomal matrix protein import by ubiquitination. (ox.ac.uk)
  • Isolation and characterisation of ligands of the yeast estrogen binding protein. (edu.au)
  • The active site subunit of the OST is the STT3 protein. (umassmed.edu)
  • Spore morphogenesis begins with the development of the prospore membrane, a double lipid bilayer that grows to surround each of the meiotic products. (umb.edu)
  • In plants, RNase III Dicer-like proteins (DCLs) act as sensors of dsRNAs and process them into short 21- to 24-nucleotide (nt) (s)RNAs. (biomedcentral.com)
  • Interestingly, the genomes of insects, plants and vertebrates encode two STT3 proteins. (umassmed.edu)
  • Deficiency of any of these regulatory proteins results in a state of overactivation of the complement system, with damaging inflammatory effects. (medscape.com)
  • A small-molecule competitive inhibitor of phosphatidic acid binding by the AAA+ protein NSF/Sec18 blocks the SNARE-priming stage of vacuole fusion. (avantilipids.com)
  • SNARE proteins are essential components of the machinery that facilitates membrane fusion in eukaryotic cells. (gla.ac.uk)
  • The yeast SM protein Vps45p regulates membrane fusion through the trans-Golgi / late endosomal system, and interacts directly with the syntaxin (Tlg2p) and the v-SNARE (Snc2p) proteins. (gla.ac.uk)
  • Together with Syntaxin-1A and SNAP25, it forms the core membrane fusion machinery that is responsible for neurotransmitter release and, therefore, signal transmission between neurons. (researchgate.net)
  • E.M. Parodi, C.S. Baker, C. Tetzlaff, S. Villahermosa, and L.S. Huang (2012) SPO71 mediates prospore membrane size and maturation in Saccharomyces cerevisiae. (umb.edu)
  • Thermotropic and enzymatic studies on yeast membranes with altered sterol compositions. (edu.au)
  • One region of this protein, called the sterol reductase domain, plays an important role in the production (synthesis) of cholesterol. (medlineplus.gov)
  • I aimed to identify RNA-binding proteins (RBPs) in yeast that associate with ER and to assess their role in mRNA localization and/or translation at ER. (uni-tuebingen.de)
  • Instead, Fus1p localization to the tip of the mating projection was determined by its cytosolic domain, which binds to peripheral proteins involved in mating tip polarization. (ox.ac.uk)
  • Alpha-COP has been shown to be required for ER localization of di-lysine-tagged proteins. (rupress.org)
  • The smaller proteins are generally of prokaryotic origin while the larger ones are of eukaryotic origin. (tcdb.org)
  • The larger eukaryotic and archaeal proteins possess N- and C-terminal hydrophilic extensions. (tcdb.org)
  • Here, we develop a highly parallel protein-protein interaction sequencing (PPiSeq) platform that uses a novel double barcoding system in conjunction with the dihydrofolate reductase protein-fragment complementation assay in Saccharomyces cerevisiae . (nature.com)
  • Formulation of unifying hypotheses describing how SM proteins function has been problematic, primarily due to the multiple modes of interaction that have been characterised for different members of this family binding to their cognate SNARE proteins. (gla.ac.uk)
  • In addition to investigating the functional significance of Vps45p's interaction(s) with Tlg2p, I have also investigated binding of the SM protein to the v-SNARE Snc2p. (gla.ac.uk)
  • He carries out multidisciplinary research, doing studies in Protein-protein interaction and Two-hybrid screening. (research.com)
  • We show the first near-atomic structure of yeast complex IV and the protein-protein, and lipid-protein interaction implicated in supercomplex formation. (lu.se)
  • The classical pathway is triggered by interaction of the Fc portion of an antibody (immunoglobulin [Ig] M, IgG1, IgG2, IgG3) or C-reactive protein with C1q. (medscape.com)
  • Current technologies, which assay one PPI at a time, are too low throughput to make it practical to study protein interactome dynamics. (nature.com)
  • To investigate whether Emp47p undergoes retrograde transport from the Golgi to the ER like other di-lysine-tagged proteins we developed an assay to measure this step after block of forward transport in a sec12 mutant. (rupress.org)
  • Igor Stagljar, the study's senior author, told ProteoMonitor this week that his team has been interested broadly in how integral membrane proteins interact at the genome-wide level. (genomeweb.com)
  • A second group of methods, which includes DNA microarrays and proteomics, have advantages that overcome the limitations implicit in signature-tagged mutagenesis and in vivo expression technology, namely, the ability to directly measure expression (gene or protein) levels on a true genome-wide scale, but their application to analysis of bacterial pathogens during real infections is still in its infancy. (cdc.gov)