Membranes hl 60 cells. Medical search. Frequent questions

Here, we have used two-color multiparameter flow cytometry to study simultaneously its effects on cell membrane permeability, intracellular pH, and cell size/structure of EMT6 mouse mammary tumor cells and HL-60 human promyelocytic leukemia cells in vitro . (aacrjournals.org)
Human promyelocytic leukemia cell line HL-60 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA) and its ABCG2-overexpressing subclone (here referred to as cBCRP) was kindly provided by Dr Balazs Sarkadi (Membrane Research Group, Hungarian Academy of Sciences, Budapest, Hungary) ( 10 ). (spandidos-publications.com)
By assaying their anti-proliferative and anti-apoptotic effects using human promyelocytic HL-60 cells, we found that the ethanolic extract of I'm-Yunity-Too was more active in inducing cell death compared to I'm-Yunity, based on measured changes in the expression of caspase 3 and Bax. (spandidos-publications.com)
The cell-surface proteins of HL-60 human promyelocytic leukemia cells have been compared to those of normal human neutrophils. (johnshopkins.edu)
Compared to human promyelocytic HL-60 cells, WEHI-3 cells and murine neutrophils expressed low α1-3-fucosyltransferase activities. (ashpublications.org)
Generated to the HL60 promyelocytic cell line, it can react with all hematopoietic myeloid cells, granulocytes and normal monocytes, as well as leukemic cells from some patients with AML, AMML, and ALL. (duke.edu)
The aim of this study was to investigate the cytodifferentiative, cytostatic and cytotoxic potential of a decoction of Hemidesmus indicus's roots (0.31-3 mg/mL) on a human promyelocytic leukemia cell line (HL-60). (unibo.it)

Differentiation of HL-60 cells by treatment with dimethylformamide, trans-retinoic acid, or 12-O-tetradecanoylphorbol acetate did not modify the predominant surface-labeled proteins of HL-60 cells to produce a pattern similar to that of normal, mature neutrophils. (johnshopkins.edu)
Skubitz, KM & August, JT 1983, ' Analysis of cell-surface protein changes accompanying differentiation of HL-60 cells ', Archives of Biochemistry and Biophysics , vol. 226, no. 1, pp. 1-9. (johnshopkins.edu)
Monoclonal antibodies reactive with myeloid precursor cells in the bone marrow or cells undergoing early myeloid differentiation are capable of detecting antigens that may then appear at discrete stages of myeloid maturation or on cells from some patients with myeloid and/ or monocytic leukemia. (duke.edu)
These antigens can thus be especially useful for the classification of leukemias and as a marker of myeloid cell differentiation. (duke.edu)
Induction of apoptosis, cell-cycle analysis, levels of specific membrane differentiation markers were evaluated by flow cytometry. (unibo.it)
The analysis of cell differentiation by nitroblue tetrazolium (NBT) reducing activity, adherence to the plastic substrate, alpha-napthyl acetate esterase activity and morphological analysis was performed through light microscopy (LM) and transmission electron microscopy (TEM). (unibo.it)
The decoction caused differentiation of HL-60 cells as shown by NBT reducing activity, adherence to the plastic substrate, alpha-naphtyl acetate esterase activity, and increasing expression of CD14 and CD15. (unibo.it)
Vitamin D3-induced monocytic differentiation resulted in an increased carboxypeptidase M expression in all three cell lines. (uni-regensburg.de)
In another 2023 study , the drug was tested on mouse lymphoma and spleen cells to see if there was differentiation between killing cancer cells and preserving healthy spleen cells. (cancer.news)

By means of cytofluorimetric analysis of pre-G1 population and caspase 3 activation, we demonstrated that Mrt-HP extracts activated apoptosis in HL-60 cell line. (cnr.it)

A dose-dependent effect on mitochondrial membrane potential loss was observed. (ejbiotechnology.info)
Green husk extract generates an antiproliferative effect in HL-60 cells, which is related to an induction of the early stages of apoptosis and a loss of mitochondrial membrane potential. (ejbiotechnology.info)
The mitochondrial membrane potential of FBZ-treated HL-60 cells decreased in a concentration-dependent manner. (kjvr.org)

Plasma membrane redox system (PMRS) is an electron transport chain system ubiquitously present in all cell types that transfers electrons from intracellular substrates to extracellular acceptors maintaining redox homeostasis for a successful cell physiology [ 1 ]. (hindawi.com)
Several compounds have been shown to inhibit the efflux activity of ABC transporters, thereby increasing intracellular drug accumulation and sensitising cancer cells to therapy. (spandidos-publications.com)
Moreover, we revealed the ability of proadifen to increase the intracellular hypericin content in HT-29 cells most likely via the inhibition of BCRP and MRP1 transporters ( 8 ). (spandidos-publications.com)
They play a role in the intracellular transport of molecules contained within membrane vesicles. (lookformedical.com)
The tendency for water to move into and out of cells is determined by the relative tonicity of intracellular and extracellular fluids. (mhmedical.com)
Because of the ability of water to pass freely across membranes, the intracellular and extracellular space have equivalent osmolality, normally about 280 mOsm/kg ( Fig. 2-1 ). (mhmedical.com)

Plasma membrane redox system (PMRS) is an electron transport chain system ubiquitously present throughout all cell types. (hindawi.com)
We found that stimulated neutrophils eject extracellular DNA after decondensation of the nuclear DNA in the nucleus, rupture of the lamin meshwork and nuclear membrane allowing the release of decondensed chromatin to the cytosol, disassembly of the actin, microtubule and vimentin intermediate filaments networks, vesiculation of the endoplasmic reticulum and plasma membrane and finally rupture of the plasma membrane. (mpg.de)
Biochemical characterization revealed that both antibodies detect the same 58-64-kDa glycoprotein anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. (uni-regensburg.de)
The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. (neobiotechnologies.com)

Curcumin, isolated from Curcuma longa, has modulatory effects on cellular physiology due to its membrane interaction ability and antioxidant potential. (hindawi.com)
Plant Cell Physiology. (sinica.edu.tw)

Proposed functions of PMRS include maintenance of redox state in proteins, stimulation of cell growth, reduction of lipid hydroperoxides, recycling of α -tocopherol, reduction of ferric ion prior to iron uptake by a transferring-independent pathway, and the maintenance of the extracellular concentration of ascorbic acid [ 2 - 6 ]. (hindawi.com)
Curcumin modulates cellular activity through interacting with membrane-bound proteins and modulating signaling cascade activity by altering the fluidity of the membrane bilayer [ 17 ]. (hindawi.com)
Multidrug resistance caused by the overexpression of ABC transporter proteins in cancer cells remains a major obstacle limiting chemotherapy efficacy. (spandidos-publications.com)
It was previously reported that proadifen (SKF-525A), a well-known cytochrome P450 monooxygenase inhibitor, not only has anti-proliferative potential in some cancer cell lines, but it is also able to inhibit BCRP and MRP1 transporter proteins ( 8 ). (spandidos-publications.com)
Proteins of HL-60 cells surface labeled with 125 I differed markedly from those of normal neutrophils, as shown by immunoprecipitation and polyacrylamide electrophoresis. (johnshopkins.edu)
However, the agents did induce greater quantities of minor cell-surface proteins immunoprecipitated by hyperimmune anti-human neutrophil serum. (johnshopkins.edu)
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. (thermofisher.com)
A family of proteins that play a role as cofactors in the process of CLATHRIN recycling in cells. (lookformedical.com)
A network of membrane compartments, located at the cytoplasmic side of the GOLGI APPARATUS, where proteins and lipids are sorted for transport to various locations in the cell or cell membrane. (lookformedical.com)
Vesicular transport proteins are distinguished from MEMBRANE TRANSPORT PROTEINS, which move molecules across membranes, by the mode in which the molecules are transported. (lookformedical.com)
CsH inhibited activation by FMLP of high affinity GTPase (the enzymatic activity of alpha-subunits of heterotrimeric regulatory guanine nucleotide-binding proteins) in HL-60 membranes with a Ki of 0.79 microM. (rspharmchem.com)
The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. (neobiotechnologies.com)

Despite high ORAC values, neither purified juglone nor the extract showed protective effects on HL-60 cells under oxidative conditions. (ejbiotechnology.info)
GSH protects the cell and biomolecules against oxidative injury, thus providing a powerful antioxidant defense mechanism against reactive oxygen species (ROS) and reactive nitrogen species (RNS) [ 12 - 14 ]. (hindawi.com)
These include regulation of cell cycle, activation of mitochondrial pathway, induction of mitochondrial outer membrane permeabilization, induction of apoptosis, modulation of oxidative stress, amelioration of inflammation, modulation of insulin signaling and inhibition of angiogenesis. (mdpi.com)
ROS의 생산과 항산화물질 작용의 불균형으로 인해 세포 내 ROS가 증가하게 되면 oxidative stress가 발생하며, ROS는 DNA, 단백질 등과 반응하여 손상시키고 cell death를 유발한다[ 10 ]. (kjvr.org)
Secondly, the drug induces oxidative stress that disrupts cell metabolic processes. (cancer.news)
The drug induced oxidative stress on the cancer cells, making them more vulnerable to apoptosis. (cancer.news)
While IAEMH is associated with severe anaemia due to intravascular haemolysis caused by red cell invasion, oxidative injury, auto-antibodies, and/or pathogen-haem interaction, IAIMH is associated with haemophagocytic tri-lineage destruction of haematopoietic precursors in the bone marrow. (bvsalud.org)

GSH is a hydrophilic antioxidant with nucleophilic thiol group and has been reported to participate in regulation of gene expression, protein synthesis, cell proliferation, signal transduction, cytokine production, apoptosis, immune response, and protein glutathionylation [ 11 ]. (hindawi.com)
Extracts prepared from Trametes versicolor under the trade name of I'm-Yunity exhibit anti-tumorigenic activities, as supported by inhibition of the proliferation and induction of apoptosis in malignant cells. (spandidos-publications.com)
High estrogen states, as seen during puberty and pregnancy, promote the preservation of a homeostatic (eubiotic) vaginal microenvironment by stimulating the maturation and proliferation of vaginal epithelial cells and the accumulation of glycogen. (frontiersin.org)
In the end, the drug decreased cell proliferation of both chemo-sensitive and chemo-resistant ovarian cancer cells. (cancer.news)

It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
We show for the first time that proadifen is able to enhance the cytotoxic properties of MTX in cBCRP cells, particularly through the inhibition of BCRP expression and activity. (spandidos-publications.com)
This is reinforced by Lactobacilli through the production of hydrogen peroxide (H 2 O 2 ), bacteriocins and biosurfactants, as well as the inhibition of the physical attachment of pathogens to the epithelium by competitive exclusion and the promotion of the engulfment and degradation of infected epithelial cells (autophagy). (frontiersin.org)
CsH inhibited FMLP binding in HL-60 membranes with a Ki (inhibition constant) of 0.10 microM. (rspharmchem.com)
Authors showed that a fraction from D. tertiolecta CCMP364 containing 95% of violaxanthin displayed 50% growth inhibition on the MCF7 breast cancer cell line at a concentration of 11.7 μg/mL. (frontiersin.org)

We performed high resolution time-lapse microscopy of mouse and human neutrophils (PMN) and differentiated HL60 neutrophil-like cells (dHL-60) labelled with fluorescent markers of the cytoskeleton and organelles and stimulated with ionomycin, lipopolysaccharides or C. albicans to induce NETosis. (mpg.de)

HL-60 cells were more sensitive to SRI 62-834 than were EMT6 cells in terms of both cytotoxicity and membrane permeability. (aacrjournals.org)
However, PAF was more potent than SRI 62-834 in causing membrane permeabilization with both cell lines, whereas PAF was less active than SRI 62-834 in cytotoxicity assays. (aacrjournals.org)
Because mitoxantrone (MTX) is a strong BCRP substrate and is often used in the treatment of leukemia, we investigated the effect of 24 h proadifen pre-treatment on the cytotoxicity of MTX in leukemic cell lines that are sensitive to MTX (HL-60) and MTX-resistant ABCG2-overexpressing subclone (cBCRP). (spandidos-publications.com)
We also demonstrated that Mrt-HP cytotoxicity was not an artifact due to cell growth conditions because addition of Mrt-HP extracts to cell medium generated peroxide levels indistinguishable from controls. (cnr.it)
Thus, this study aims to evaluate the biological activity of different essential oils (EOs) on Leishmania ( L .) amazonensis promastigotes forms, as well as their cytotoxicity on mammalian cells and chemical composition. (biomedcentral.com)
Sixteen EOs were evaluated by mean of IC 50 /24 h and cytotoxicity against L6 cells (CC 50 /24 h) using Resazurin assay. (biomedcentral.com)
RESULTS: Starting from the concentration of 0.31 mg/ml, Hemidesmus indicus induced cytotoxicity and altered cell-cycle progression, through a block in the G0/G1 phase. (unibo.it)

Another study from 2022 provides evidence that benzimidazoles induce apoptosis and pyroptosis of human glioblastoma (GBM) cancer cells. (cancer.news)

Since radiation-induced DNA damage is generated essentially randomly in the genome, it does not seem likely that radiation could ever be responsible for the induction of identical translocations of relevance to ALL in multiple cells of an individual and hence be the primary cause of radiation-related leukemia. (bioone.org)
We demonstrated that a lyophilized preparation of Mrt-HP beer was cytotoxic with respect to a beer with low polyphenolic content (Trt-LP) when assayed on HL-60 human leukemia cell line. (cnr.it)
In this study, we investigated whether FBZ has anti-cancer activity in HL-60 cells, a human leukemia cell line, and assessed its relationship with the production of reactive oxygen species (ROS). (kjvr.org)
Fenbendazole, for example, possesses at least twelve anti-cancer mechanisms of action and has been proven effective against a host of different cancers, including colorectal cancer cells, leukemia, breast cancer, lymphomas and ovarian cancer. (cancer.news)

We observed that P- and E-selectin bound to pronase-sensitive ligands on murine monocytic WEHI-3 cells and murine neutrophils, indicating that the ligands for both selectins are glycoproteins. (ashpublications.org)
CSLEX-1, HECA-452, and other widely used mAbs to sLe x and Le x did not bind to WEHI-3 cells and bound at very low levels to murine neutrophils. (ashpublications.org)
Consistent with very low endogenous fucosylation, forced fucosylation of intact WEHI-3 cells or murine neutrophils by exogenous α1-3-fucosyltransferase FTVI and GDP-fucose created many new epitopes for anti-sLe x mAbs such as HECA-452 and CSLEX-1. (ashpublications.org)

Concentrations as low as 1 µ m up to 100 µ m SRI 62-834 caused a rapid, dose-dependent increase in membrane permeability, initially towards outward efflux of the preloaded fluorescein probe bis(carboxyethyl)carboxyfluorescein (green fluorescence) and then towards influx of extracellular propidium (red fluorescence). (aacrjournals.org)
The main advantage of hydrogels for microfluidic cell cultures, however, is their mimicry of extracellular matrix structures including adequate porosity for cellular organization, biocompatibility, and representative stiffness, all key parameters that promote native-like tissue function. (heraldopenaccess.us)
Despite recent achievements of microfluidic 2D cell culture systems [9], they still do not address the fact that in vivo cells coexist in 3D communities that are influenced by spatial orientation of cells and cell-to-cell contact within the extracellular matrix [10]. (heraldopenaccess.us)
Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. (lookformedical.com)
An increase in the urea concentration in extracellular fluid increases the osmolality of the extracellular fluid but does not increase the tonicity of the extracellular fluid or cause a net movement of water out of cells. (mhmedical.com)

Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
however, it is thought to be partly due to physiological changes occurring in tumour cell membranes which reduce their adhesion to other cells. (boghammar.se)

Fucoidan treatment of HL-60 cells could change the mitochondrial membrane permeability. (fucoxanthin.us)
Diabetes causes retinal microvasculopathy associated with pericyte cell death, microaneurysms, abnormal vascular permeability, and macular edema. (molvis.org)

The development of technology to produce monoclonal antibodies for the immunological detection of cell membrane antigens has been extensively applied to the identification of human lymphocyte cell surface antigens. (duke.edu)
This invention is a murine monoclonal antibody used to detect cell membrane antigens specific for human myeloid cells. (duke.edu)
The two monoclonal antibodies MAX.1 and MAX.11 recognize cell surface antigens that are almost undetectable on monocytes but highly expressed on differentiated macrophages. (uni-regensburg.de)

In addition, carboxypeptidase M expression could be detected in HL-60, U937, and THP-1 myeloid cell lines. (uni-regensburg.de)

BCRP expression was detected in several different solid tumours ( 1 - 5 ), as well as in malignant hematopoietic and lymphoid cells ( 6 ). (spandidos-publications.com)

The combination of 3-dimensional hydrogel cell cultures with microchip technology further allows (i) spatial and temporal control of cell growth, (ii) application of defined mechanical (e.g. shear, strain, stretch) and chemical (e.g. gradients) stimuli, as well as (iii) monitoring of dynamic cellular responses using integrated sensing strategies. (heraldopenaccess.us)
CsH and BocPLPLP did not inhibit the rises in [Ca2+]i induced by several other stimuli in HL-60 cells and HEL cells. (rspharmchem.com)
Inflammation-associated tissue injury may activate stem/progenitor cells, and mutagenic stimuli from inflammation can accumulate multiple mutations and epigenetic changes in stem/progenitor cells [ 3 , 10 ]. (hindawi.com)
May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli. (neobiotechnologies.com)

In addition, Hoechst 33342 staining showed the presence of broken nuclei in HL-60 cells treated with 0.5 and 1 μM FBZ. (kjvr.org)
DSRCT is composed of small cells with round hyperchromatic nuclei and a dense fibrous or spindle cell stroma. (medscape.com)

Endosomes internalize macromolecules bound by receptors on the cell surface. (lookformedical.com)

Protein activation and expression were analysed by western blotting, deoxyribonucleoside triphosphate levels by HPLC, ribonucleotide reductase activity by 14 C-cytidine incorporation into nascent DNA and cell-cycle distribution by FACS. (nature.com)
Specialized regions of the cell membrane composed of pits coated with a bristle covering made of the protein CLATHRIN. (lookformedical.com)
An adaptor protein complex primarily involved in the formation of clathrin-related endocytotic vesicles (ENDOSOMES) at the CELL MEMBRANE. (lookformedical.com)
The VEGF-A protein and mRNA levels in conditioned medium of RGC-5 cells incubated with AGE-modified BSA (AGE-BSA) were examined with real-time PCR and enzyme-linked immunosorbent assay (ELISA), and BSA-treated cells were used as controls. (molvis.org)
Compared with the BSA controls, the RGC-5 cells incubated with AGE-BSA showed a dose- and time-dependent increase in VEGF-A mRNA and VEGF-A protein secretion in the supernatant, with the highest levels achieved at 24 h. (molvis.org)
As the drug blocked cell cycle progression, it increased p53 protein stability and induced apoptosis of the cancer cells. (cancer.news)
CD97 is a G-protein-coupled seven-span transmembrane adhesive receptor that is constitutively expressed on granulocytes and monocytes and rapidly upregulated on T and B cells upon activation. (thermofisher.cn)
In the Kcne2 potassium channel ancillary subunit knockout model, disruption of this gene induces achlorhydria and is related to reduced parietal cell protein secretion and abnormal parietal cell morphology. (medscape.com)
Lumbar puncture revealed a white blood cell count of 23 cells/ µ L (normal: 0 cells/ µ L) with 93% lymphocytes, a red blood cell count of 3 cells/ µ L (normal: 0 cells/ µ L), a protein concentration of 50 mg/dL (normal: 15--45 mg/dL), and a glucose concentration of 58 mg/dL (normal: 40--70 mg/dL). (cdc.gov)

applied fucoidan to the human colon cancer DLD-1 and found that it can inhibit tumor cell multiplication by suppressing the activity of epidermal growth factors. (fucoxanthin.us)

1 , 2 P- and E-selectin, expressed on activated platelets and/or endothelial cells, bind to ligands on leukocytes. (ashpublications.org)
L-selectin, expressed on leukocytes, binds to ligands on some endothelial cells and on other leukocytes. (ashpublications.org)
Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. (neobiotechnologies.com)
We present a rate equation model for the TGF-b pathway in endothelial cells together with novel measurements. (lu.se)
endothelial cells. (lu.se)

These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)

At puberty and during pregnancy, elevated levels of estrogen promote the maturation of and deposition of glycogen in vaginal epithelial cells. (frontiersin.org)
Glycogen from exfoliated and lysed epithelial cells is catabolized by α-amylase in the vaginal lumen to smaller polymers that are subsequently metabolized to lactic acid by Lactobacillus spp. (frontiersin.org)
Lactic acid and cytolysin produced by Lactobacilli stimulate the dissolution of epithelial cells by lysis and enhance the availability of glycogen. (frontiersin.org)
Similarly, more intense staining of CD44v6 and ALDH1A1 was detected in an NPC cell line than in an immortalized nasopharyngeal epithelial cell line. (hindawi.com)
Acid secretion by gastric epithelial cells is related to the physiologic function of oxyntic cells, which are called parietal cells. (medscape.com)
Shh acts as a signaling molecule in the stomach epithelial patterning, and loss of Shh results in defective parietal cells, leading to hypochlorhydria and subsequent hypergastrinemia. (medscape.com)
The increased incidence of cancer with age is thought to be attributable to: At least 70% of cancers occur in epithelial cells which are constantly exposed to external, ingested or inhaled substances. (boghammar.se)
Synovial sarcomas encompass both biphasic (epithelial and spindle cell components) and monophasic (purely spindle cell) variants. (medscape.com)

The normal cells were not affected when juglone is present at concentrations of 1 μM, while at higher concentrations, there is loss of viability of both cancerous and healthy cells. (ejbiotechnology.info)
The application of hydrogels as a matrix for 3-dimensional cell cultures has become an indispensable tool in tissue engineering, biotechnology and biomedical research due to the improved functionality and viability of the in vitro biological system. (heraldopenaccess.us)
We measured a 60% decrease in cell viability at a polyphenol concentration of 250 μM quercetin equivalents. (cnr.it)
Genetic ablation of a Na + /H + exchanger (NHE2), which is expressed in the stomach at high levels, leads to a decrease in gastric acid secretion, along with decreased viability of parietal cells, severe metaplasia, and hyperplasia of the gastric mucosa. (medscape.com)

During inflammation, binding of selectins to cell-surface glycoconjugates mediates rolling adhesion of leukocytes on vascular surfaces. (ashpublications.org)
Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. (neobiotechnologies.com)

The staining intensity of 8-nitroguanine was significantly higher in cancer cells and inflammatory cells in the stroma of NPC tissues than in chronic nasopharyngitis tissues. (hindawi.com)
Expression levels of CD44v6 and ALDH1A1 were significantly increased in cancer cells of primary NPC specimens in comparison to chronic nasopharyngitis tissues. (hindawi.com)
Chronic inflammatory changes related to gastric Helicobacter pylori infection can also induce parietal cell changes. (medscape.com)
Romero-Pozuelo J, Dason JS , Atwood HL , FerrÃºs A. Chronic and acute alterations in the functional levels of Frequenins 1 and 2 reveal their roles in synaptic transmission and axon terminal morphology. (neurotree.org)
Infants suffering of cell death occurring as a series of changes from chronic illnesses, chromosomal abnor- in dying cells under several physiological malities and those on medication prior to conditions [ 6,7 ] and has been implicated enrolment were excluded from the study. (who.int)
Sickle cell disease (SCD) is associated with chronic haemolysis, immuno-suppression and suscepti- bility to infections, which may trigger infection-associated haemolysis (IAH). (bvsalud.org)

beta, gamma-imino]triphosphate, p[NH]ppG, and the regulation of binding by the formyl-peptide-receptor agonist, fMet-Leu-Phe, were studied in membranes of differentiated HL-60 cells. (eurekamag.com)
My lab studies the mechanism and regulation of immune checkpoints, using a combination of biophysical, cell biological and immunological approaches. (mpg.de)
CD97 is involved in T cell regulation and peripheral granulocyte homeostasis. (thermofisher.cn)

These SP constitute an important ingredient of cell walls and get harvested by suitable extraction or precipitation method, followed by purification, characterization and biological studies (Fig. 2 ). (springer.com)
Within this work, and considering the discussed background, another D. tertiolecta clone (CCMP 1320) was selected and tested against several cancer cell lines, and a bioactivity-guided fractionation approach allowed to identify the source of the biological activity observed within the extracts of this green alga. (frontiersin.org)
Modeling biological membranes with circuit boards and measuring electrical signals in axons: student laboratory exercises. (neurotree.org)
Increasing knowledge about newer biological factors such as HER2/neu receptors and proliferative index (rate of cell division in the tumour) has altered the way adjuvant treatments (see p. 812) are prescribed and these indicators are increasingly used to predict prognosis. (boghammar.se)
The aim of this study was to evaluate in vivo the biological response after implantation of a novel alginate-capsule membrane. (bvsalud.org)

In conclusion, CD44v6 and ALDH1A1 are candidate stem cell markers for NPC, and the increased formation of DNA lesions by inflammation may result in the mutation of stem cells, leading to tumor development in NPC. (hindawi.com)

In our previous study, we demonstrated the anti-proliferative and pro-apoptotic activity of proadifen in the HT-29 cancer cell line ( 9 ). (spandidos-publications.com)

CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. (neobiotechnologies.com)

Additionally, juglone is present in the green husk of walnut, which suggests evaluating the effect of GH extracts on carcinogenic cell lines. (ejbiotechnology.info)
In the current study, the antiproliferative activity of raw extracts and fractions of D. tertiolecta (clone CCMP 1320) was evaluated against four different human cancer cell lines: melanoma, hepatocellular liver carcinoma, and two lung adenocarcinoma cell lines. (frontiersin.org)

It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. (neobiotechnologies.com)

In vitro antitumor activity was seen over the same concentration range, as measured by tetrazolium dye reduction and cell growth curves. (aacrjournals.org)

Under near-infrared (NIR) light irradiation, PDT generates reactive oxygen species (ROS) that precisely damage the mitochondria of tumor cells and break single-stranded DNA (ssDNA) to activate MCD@TMPyP4@DOX self-disassembly for release of DOX and DNAzyme. (bvsalud.org)
There is also evidence that benzimidazole drugs sensitize tumor cells so they can become more susceptible to radiation therapy. (cancer.news)

The EO from F. galbaniflua showed to be effective against L. amazonensis promastigotes forms and presented low cytotoxic activity against L6 cells. (biomedcentral.com)

FBZ treatment at 0.25-1 μM significantly decreased the metabolic activity of HL-60 cells. (kjvr.org)
NAC significantly recovered the decreased metabolic activity of HL-60 induced by 0.5 and 1 μM FBZ treatments. (kjvr.org)

Because MTX is a preferential and strong substrate of BCRP, MTX-sensitive HL-60 cell line and an MTX-resistant ABCG2-overexpressing subclone (here referred to as cBCRP) were chosen as the experimental models. (spandidos-publications.com)
실험에서 사용한 HL-60 세포는 한국세포주은행(Korean Cell Line Bank, Korea)에서 구입하였으며, 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin/streptomycin이 포함된 RPMI 1640 세포배지를 이용해 배양하였다. (kjvr.org)
In this study, we explore the role of HMGB1 in advanced glycation end products (AGEs)-induced vascular endothelial growth factor A (VEGF-A) production in rat retinal ganglion cell line 5 (RGC-5) cells. (molvis.org)
HMGB1 is implicated in the production of VEGF-A in retinal ganglion cell line-5 (RGC-5). (molvis.org)
In addition, a normal cell line (lung fibroblast) was used as toxicity control. (frontiersin.org)

Anti-/pro-oxidant effects of phenolic compounds in cells: are colchicine metabolites chain-breaking antioxidants? (cdc.gov)
Using our fluorescence-HPLC assay with metabolically incorporated oxidation-sensitive cis-parinaric acid (PnA) we studied the effects of colchicine metabolites on peroxidation of different classes of membrane phospholipids in HL-60 cells. (cdc.gov)
It can prevent the growth of cancer cells along with immune-modulating and antiviral effects. (fucoxanthin.us)
These effects of honey have been thoroughly investigated in certain cancers such as breast, liver and colorectal cancer cell lines. (mdpi.com)
We studied the effects of CsH in comparison with those of N-t- butoxycarbonyl-L-phenylalanyl-L-leucyl-L-phenylalanyl-L-leucyl-L- phenylalanine (BocPLPLP), a well known formyl peptide receptor antagonist, and of other Cs on activation of N6,2'-O-dibutyryl adenosine 3:5'-monophosphate-differentiated HL-60 cells and human erythroleukemia cells (HEL cells). (rspharmchem.com)
Inhibitory effects of tetrandrine and hernandezine on Ca2+ mobilization in rat glioma C6 cells. (mcmaster.ca)
Zhang W, Tan YF, Atwood HL , Martin Wojtowicz J. Biphasic effects of the cholinergic agonist carbachol on long-term potentiation in the dentate gyrus of the mammalian hippocampus. (neurotree.org)

The NH2-terminal amino acid sequence was determined and turned out to be identical to the NH2-terminal sequence of the membrane-bound carboxypeptidase M. By precipitation with antibodies MAX.1 and MAX.11, membrane preparations of macrophages and placental microvilli were almost completely depleted of enzyme activity, indicating that the two antibodies indeed recognize carboxypeptidase M. Immunoreactivity of both antibodies correlates with the reported tissue distribution of enzyme activity. (uni-regensburg.de)

In clinical conditions, parietal cell dysfunction can be induced by antiparietal cell antibodies. (medscape.com)
The clinical consequences of infection with Prospect Hill virus, which has been identified in the United States, are unknown, but antibodies have been detected in humans who could not recall an illness typical of HFRS (11). (cdc.gov)

Furthermore, di-GA inhibited the generation of lymphendothelial gaps by cancer cell spheroid-secreted lipoxygenase metabolites. (nature.com)
Fucoidan from Cladosiphon navae-caledoniae plus anti-cancer agents reduced the ERK phosphorylation in MDA-MB-231 breast cancer cells compared to untreated control or fucoidan alone. (fucoxanthin.us)
They found that apoptosis of colon cancer cells induced by fucoidan is regulated by the death of receptor-mediated apoptotic pathways. (fucoxanthin.us)
Bladder cancer is a common type of cancer that begins in the cells of the bladder. (fucoxanthin.us)
first reported the impact of fucoidan on the growth of bladder cancer cells. (fucoxanthin.us)
To investigate the anti-cancer mechanism of FBZ, HL-60 cells were treated with FBZ in the absence or presence of N-acetyl cysteine (NAC), an inhibitor of ROS production. (kjvr.org)
This study provides evidence that FBZ has anti-cancer activity in HL-60 cells provided, in part, via ROS production. (kjvr.org)
Abrogation of G2 checkpoint specifically sensitize p53 defective cells to cancer chemotherapeutic agents. (mcmaster.ca)
To investigate the involvement of stem cells in Epstein-Barr virus infection-related nasopharyngeal carcinoma (NPC), we used double immunofluorescence staining to examine several cancer stem/progenitor cell markers (CD44v6, CD24, and ALDH1A1) in NPC tissues and NPC cell lines. (hindawi.com)
Our previous study was the first to demonstrate 8-nitroguanine formation in the cancer cells of NPC patients via iNOS activation [ 14 ], showing that inflammation is an important risk factor for NPC development. (hindawi.com)
rather, this property is limited to a subset of cells termed "cancer stem cells" [ 15 , 16 ]. (hindawi.com)
Moreover, several studies have shown that cancer cells have genetic instability, epigenetic changes, and an accumulation of mutations, suggesting that cancer is a genetic disease [ 16 ]. (hindawi.com)
In the study, " Repurposing anti-parasite benzimidazole drugs as selective anti-cancer chemotherapeutics (2023)," researchers found that Fenbendazole has two primary mechanisms of action against cancer cells. (cancer.news)
It inhibits glucose uptake, suppresses key enzymes and reduces the level of available ATP energy, leading to apoptosis of both parasites and cancer cells. (cancer.news)
In the study, " Redox-mediated Anticancer Activity of Anti-parasitic Drug Fenbendazole in Triple-negative Breast Cancer Cells," researchers tested fenbendazole on triple negative breast cancer cells, including a highly metastatic type. (cancer.news)
The drug proves to be less toxic to healthy cells than traditional chemotherapy agents, yet similarly lethal to the cancer cells. (cancer.news)
In a 2022 study, fenbendazole was tested against chemo-resistant colorectal cancer cells . (cancer.news)
It also induces apoptosis by Beclin-1 and causes cell cycle arrest of cancer cells at the G2/M phase. (cancer.news)
In addition, the drug causes autophagy, necroptosis and ferroptosis, killing off even the most resistant cancer cells. (cancer.news)

In HL-60 leukaemia cells, di-GA activated caspase 3 and dose-dependently induced apoptosis. (nature.com)

The research showed that fenbendazole induces reactive oxygen species and causes cell death of lymphoma cells but does not harm the normal spleen cells. (cancer.news)

In addition, PMRS also regulates physiological functions like cell metabolism, cellular growth, activity of ion channels, and cell death against changes in redox potential [ 7 , 8 ]. (hindawi.com)

McGraw Hill, 2014, https://accessbiomedicalscience.mhmedical.com/content.aspx?bookid=843§ionid=48779649. (mhmedical.com)
McGraw Hill, 2019, https://accessanesthesiology.mhmedical.com/content.aspx?bookid=2508§ionid=202424113. (mhmedical.com)

The aim of this study was to test for the molecular and functional expression of the non-gastric H + /K + ATPase ATP12A/ATP1AL1 in unstimulated and butyrate-stimulated (1 and 10 mM) human myelomonocytic HL-60 cells, to unravel its potential role as putative apoptosis-counteracting ion transporter as well as to test for the effect of the H + /K + ATPase inhibitor SCH28080 in apoptosis. (karger.com)
These microfluidic cell cultures are considered potential candidates to provide next generation cell analysis systems. (heraldopenaccess.us)
These cells are defined as tumor-initiating cells or rare cells with indefinite potential for self-renewal that drives tumorigenesis [ 15 ]. (hindawi.com)

Anatomy54

Organisms12

Diseases3

Chemicals and Drugs99

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes56

Disciplines and Occupations4

Technology, Industry, Agriculture7

Information Science3

Health Care1

Promyelocytic7

Differentiation9

Apoptosis in HL-601

Mitochondrial membrane potential3

Intracellular6

Plasma membrane4

Physiology2

Proteins12

Oxidative7

Proliferation4

Inhibition5

Human neutrophils1

Cytotoxicity7

Induce apoptosis1

Leukemia4

Neutrophils3

Extracellular5

Tumour cell2

Permeability2

Antigens3

Myeloid1

Hematopoietic1

Stimuli4

Nuclei2

Receptors on the cell surface1

Protein9

Inhibit1

Endothelial cells5

Induction1

Epithelial8

Viability4

Adhesion2

Chronic6

Regulation3

Biological5

Mutation1

Anti-proliferative1

Monocytes1

Extracts2

Lymphocytes1

Vitro1

Tumor cells2

Cytotoxic1

Metabolic2

Line5

Effects7

Macrophages1

Antibodies2

Cancer19

Caspase1

Lymphoma1

Physiological1

McGraw Hill2

Potential3