• molecule
  • What this means is that the ion concentrations within the cytosol (or equivalently, the ion gradients across the cell membrane) are set by how much energy can be extracted from hydrolyzing a molecule of ATP. (physicsforums.com)
  • Hydrolyzing ATP provides energy (free energy) as [ATP]/[ADP] falls to equilibrium- 57 kJ/mol, or 590 mV, assuming a single ATP molecule gives all it's energy to a single charge (the calculation is correct, but I can't account for the discrepancy with the resting potential). (physicsforums.com)
  • This molecule (dye) intercalates among the lipophilic part of biological membranes. (wikipedia.org)
  • calcium
  • Peter Klock Hepler HonFRMS is the Constantine J. Gilgut and Ray Ethan Torrey Professor Emeritus in the Biology Department of the University of Massachusetts at Amherst who is notable for his work on elucidating the roles of calcium, membranes and the cytoskeleton in plant cell development and cell motility. (wikipedia.org)
  • intracellular
  • Among other roles, the cell potential acts as a reservoir for metabolic energy, which cells use to drive the transport of solute molecules across the membrane, to communicate with other cells and to trigger intracellular events. (academickids.com)
  • imaging parameters, such as excitation wavelength, emission wavelength, exposure time, should also be optimized Voltage-sensitive dyes often fail to penetrate through connective tissue or move through intracellular spaces to the region of membrane desired for study. (wikipedia.org)
  • ATPase
  • The proton motive force (pmf) across the lysosomal membrane is generated by a V-type ATPase which hydrolyzes cytoplasmic ATP to pump protons into the lysosomal lumen. (wikipedia.org)
  • dyes
  • Membrane potential can be determined in individual cells from the Nernstian distribution of cationic dyes. (springer.com)
  • Fast-response probes: These are amphiphilic membrane staining dyes which usually have a pair of hydrocarbon chains acting as membrane anchors and a hydrophilic group which aligns the chromophore perpendicular to the membrane/aqueous interface. (wikipedia.org)
  • proteins
  • The membrane potential is negative because usually cells have a net negative charge due to leakiness of potassium channels and the large size of negatively charged macromolecules such as proteins and RNA. (academickids.com)
  • continuously
  • On the other hand, it has been continuously reported the existence of the membrane potential inexplicable by the GHK equation by a small number of researchers [ 1 , 2 ]. (mdpi.com)
  • action potential initiation
  • We argue here that one can retain this highly detailed input structure while dramatically reducing the overall system dimension if one is content to accurately reproduce the associated membrane potential at a small number of places, e.g., at the site of action potential initiation, under subthreshold stimulation. (pubmedcentralcanada.ca)
  • Such multisite recordings may provide precise information on action potential initiation and propagation (including direction and velocity), and on the entire branching structure of a neuron. (wikipedia.org)