Mediates antibodies tnf receptor associated factor 1. Medical search. Frequent questions

Current cancer immunotherapy treatments use monoclonal antibodies (mAbs) to target and kill cancer cells. (wikipedia.org)
2] demonstrated that functional ABCB1 inhibited the activation of caspase-8 and -3 following Fas ligation and this inhibitory effect could be reversed by ABCB1 antagonists, such as specific anti-ABCB1 monoclonal antibodies. (ibs-italy.info)
Mouse monoclonal antibodies (MAbs) against caspase-8 (Ab-3), caspase-9 (F-7), and caspase-3 (E-8), as well as rabbit polyclonal antibody against ABCB1 (MDR1), bcl-2 and bak were purchased from Santa-Cruz Biotechnology (Santa Cruz, Calif, USA). (ibs-italy.info)
Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.co.jp)
Monoclonal antibodies targeting the immune regulatory "checkpoint" receptors programmed death receptor 1 (PD-1), its ligand, PD-L1, and cytotoxic T cell associated antigen 4 (CTLA-4) have demonstrated remarkable efficacy against advanced cancers [ 1 ]. (bmj.com)
Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. (abcam.com)
Moreover response to inhibitors with different mechanisms of action such as soluble receptors or monoclonal antibodies is usually heterogeneous. (researchassistantresume.com)
Murine monoclonal antibodies are produced by injecting a mouse with an antigen, harvesting its spleen to obtain B cells that are producing antibody specific to that antigen, fusing those cells with immortal mouse myeloma cells, growing these hybridoma cells (eg, in cell culture), and harvesting the antibody. (msdmanuals.com)
To minimize the problems due to use of pure mouse antibody, researchers have used recombinant DNA techniques to create monoclonal antibodies that are part human and part mouse. (msdmanuals.com)
Chimeric monoclonal antibodies activate antigen-presenting cells (APCs) and T cells more effectively than murine monoclonal antibodies but can still induce production of human anti-chimeric antibodies. (msdmanuals.com)
Fully human monoclonal antibodies are produced using transgenic mice that contain human immunoglobulin genes or using phage display (ie, a bacteriophage-based cloning method) of immunoglobulin genes isolated from human B cells. (msdmanuals.com)

Tumor necrosis factor-α (TNF-α), an important proinflammatory cytokine, exerts a variety of physiologic and pathogenic effects that lead to tissue destruction. (molvis.org)
Here, we report that the central proinflammatory cytokine TNF-α acts as a diffusible mediator of the bystander effects induced by macrophages, an effect caused by a proliferation of macrophages that trigger epithelial cell production of Netrin-1, a neuronal guidance molecule. (aacrjournals.org)
To directly examine this question, in a previous study, we exposed BEC to microglia-conditioned media in vitro and found that a microglia-conditioned medium from activated microglia directly promoted BEC proliferation, and these effects are largely attributable to the microglial cytokine TNF-α. (biomedcentral.com)
BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. (wikidoc.org)
This cytokine is a ligand for receptors TNFRSF13B /TACI, TNFRSF17 /BCMA, and TNFRSF13C /BAFF-R. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. (wikidoc.org)
Explicit immunological effects of blocking TNF are not yet certain as complex interactions within the cytokine network that support the ongoing inflammation are not fully understood. (researchassistantresume.com)
Other cytokines that signal through receptors containing gp130 are Interleukin-11 (IL-11), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine (CLC), leukemia inhibitory factor (LIF), oncostatin M (OSM), Kaposi's sarcoma associated herpes virus interleukin 6 like protein (KSHV-IL6). (dadamo.com)
In vitro studies have also demonstrated that certolizumab pegol, unlike other TNFα-inhibitors, does not cause activated peripheral blood lymphocytes to undergo apoptosis and that it inhibits lipopolysaccharide-induced cytokine production to a greater extent than other TNFα-inhibitors. (creativebiolabs.net)

The CD137 ligand is normally expressed at low levels, but can have increased expression in presence of pathogen associated molecular patterns (PAMPs) or proinflammatory immune responses like IL-1 secretion. (wikipedia.org)
5% false discovery rate) between risk of MACE and eight proteins: matrix metalloproteinase (MMP)-12, IL-27 subunit α (IL-27a), kidney injury molecule (KIM)-1, fibroblast growth factor (FGF)-23, protein S100-A12, TNF receptor (TNFR)-1, TNFR-2 and TNF-related apoptosis-inducing ligand receptor (TRAIL-R)2. (springer.com)
AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. (origene.com)
The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. (origene.com)
CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. (rndsystems.com)
Son principal ligand, le CD154, appartient à la famille des TNFs (Tumor necrosis factor). (umontreal.ca)
Its main ligand, the CD154, belongs to the Tumor necrosis factors (TNFs) superfamily. (umontreal.ca)
Its ligand, known as gp34, is a type-2 transmembrane glycoprotein member of the tumor necrosis factor (TNF) superfamily, expressed on activated T and B cells, dendritic cells and macrophages. (beckman.com)
B-cell activating factor ( BAFF ) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene . (wikidoc.org)
[4] BAFF is the natural ligand of three unusual tumor necrosis factor receptors named BAFF-R (BR3), TACI (transmembrane activator and calcium modulator and cyclophilin ligand interactor), and BCMA (B-cell maturation antigen), all of which have differing binding affinities for it. (wikidoc.org)

Various cell types can process and present these antigens to T cells, or antigens may be soluble and bound to B-cell receptors. (medscape.com)
Results In vitro, PR3 promoted monocyte-derived MGC formation using cells from patients with GPA but not from patients with MPA, and this was dependent on soluble interleukin 6 (IL-6), as well as monocyte MAC-1 and protease-activated receptor-2, found to be overexpressed in the cells of patients with GPA. (bmj.com)
TNFRII antibody was raised in Mouse using recombinant soluble human TNFRII-Fc fusion protein as the immunogen. (antibodies-online.com)
Lower level of BAFF transcripts (or a higher level of soluble BAFF) show a higher risk of producing donor-specific antibodies in the investigated patients. (wikidoc.org)
Biological Function Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form (PubMed:9034191). (qedbio.com)
Certolizumab pegol binds and neutralizes both soluble and transmembrane TNFα and inhibits signaling through both the p55 and p75 TNFα receptors in vitro . (creativebiolabs.net)

Its alternative names are tumor necrosis factor receptor superfamily member 9 (TNFRSF9), 4-1BB, and induced by lymphocyte activation (ILA). (wikipedia.org)

TNF-α-mediated bystander assays used a murine coculture system of primary colonic epithelial cells and E. faecalis -infected macrophages ( in vitro ), with an interleukin 10 (IL-10)-deficient mouse model of colon cancer that involves long-term colonization with E. faecalis ( in vivo ). (aacrjournals.org)
A colorectal cancer model was used to decipher the molecular mechanism of BF-rTK + GCV (bifidobacterial recombination thymidine kinase/ganciclovir) using a human apoptosis antibody array kit in a murine cancer model in vivo . (biomedcentral.com)
We show that the application of compressive stress to cultured murine tracheal epithelial cells elicits the increased phosphorylation of extracellular signal-regulated kinase (ERK) and Akt through an epidermal growth factor receptor (EGFR)-dependent process, consistent with previous observations of the bronchoconstrictioninduced activation of EGFR in both human and murine airways. (elsevierpure.com)
The complementarity determining regions (CDRs) from the murine antibody were then inserted into a human Fab' IgG framework, along with several other framework residues of the variable domain that were essential for maintenance of affinity. (creativebiolabs.net)
The demonstration of antibodies, mixed T-helper (Th) type 1/2 responses diisocyanate occupational asthma in a murine model after sub- and the involvement of CD8 T lymphocytes in chronic inhalation exposure at relevant exposure levels should diisocyanate OA. (cdc.gov)
However, murine models, including knockout and transgenic mice, have been useful in understanding the mechanisms of B lymphopoiesis, B-cell differentiation, and antibody formation. (medscape.com)

Western blot analysis of BCMA: Lane 1: MW marker, Lane 2: Mouse BCMA Recombinant Protein. (enzolifesciences.com)
CD137, a member of the tumor necrosis factor (TNF) receptor family, is a type 1 transmembrane protein, expressed on surfaces of leukocytes and non-immune cells. (wikipedia.org)
Addition of the 80-protein assay to established risk factors improved discrimination in the separate test sample from 0.686 (95% CI 0.682, 0.689) to 0.748 (95% CI 0.746, 0.751). (springer.com)
The protein encoded by this gene is a member of the TNF-receptor superfamily. (origene.com)
Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. (origene.com)
The TWEAK Receptor is a ~14 kDa type I transmembrane protein that is the 12A family member of the tumor necrosis factor receptor superfamily (TNFRSF12A). (bdbiosciences.com)
Mechanistically, HD-13 activated mitogen-activated protein kinase (MAPK) and nuclear factor kB (NF- κ B) signals. (hindawi.com)
A Toll-like receptor (TLR) is a transmembrane protein encoded by the Toll gene, which is one of cell transmembrane receptors and pathogen pattern recognition receptors (PRRs) in the innate immune system [ 9 ]. (hindawi.com)
It has been reported that various hosts recognize lipopolysaccharides in the cell membrane structure through TLR9 and activate downstream mitogen-activated protein kinase (MAPK) and nuclear transcription factor- κ B (NF- κ B) pathways mediated by MyD88 to promote the MAPK protein phosphorylation, and NF- κ B enters the nucleus, further releasing cellular inflammatory factors [ 12 ]. (hindawi.com)
Recombinant rat TNF alpha protein ( ab9756 ) can be used as a positive control in WB. (abcam.co.jp)
All these ligands act as homotrimers (i.e. three of the same molecule) interacting with homotrimeric receptors, [6] although BAFF has been known to be active as either a hetero- or homotrimer (can aggregate into 60-mer depending on the primary structure of the protein). (wikidoc.org)
The expression of tumor necrosis factor (TNF)-α at mRNA and protein levels significantly increased in ATP-stimulated iMG cells from patients with fibromyalgia compared to cells from healthy individuals. (nature.com)
Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. (affbiotech.com)
TRAIL, also called Apo2L, is a cytotoxic protein that induces apoptosis of many transformed cell lines but not of normal tissues, even though its death domain-containing receptor, DR4, is expressed on both cell types. (embl-heidelberg.de)
IL-6 mediates its responses through cell surface receptors that include the transmambrane protein gp130. (dadamo.com)
IL-6 triggers the formation of protein complexes of gp130 and the IL-6 receptor activating the receptor. (dadamo.com)
Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein (PubMed:12441351). (qedbio.com)
TACE also processes other cell surface proteins, including TNF receptor, TGFalpha, L-selectin, and alpha-cleavage of amyloid protein precursor (APP). (qedbio.com)
MCP-1 monocyte chemoattractant protein type 1 workplace. (cdc.gov)
Its activation is tightly controlled by numerous other signaling proteins including protein kinase C (PKC), Sab/SH3BP, and caveolin-1. (medscape.com)

The innate immune response by myeloid cells occurs as a succession of events starting at signaling through cytosolic or surface PRRs, followed by effector responses including the release of cytokines, reactive oxygen species (ROS), reactive nitrogen species (RNS), antibacterial peptides and degranulation ( Figure 1 ). (frontiersin.org)
Pathogen clearance is mediated by mechanisms such as phagocytosis, respiratory burst with the production of ROS and RNS and release of bacteriostatic peptides, but also through the cell-extrinsic initiation of inflammation via the release of proinflammatory cytokines and chemokines ( 24 ). (frontiersin.org)
[ 1 ] Neutrophils move to the site of invasion by means of chemotaxis, which occurs in response to microbial products, activated complement proteins, and cytokines. (medscape.com)
Among T cells, CD30 is preferentially expressed on a subset of T cells producing Th2-type cytokines and on CD4 + /CD8 + thymocytes that co-express CD45RO and IL-4 receptor. (rndsystems.com)
There is little understanding about how inflammatory cytokines mediate bystander effects, but questions in this area are important because of the pivotal contributions made by inflammatory processes to cancer initiation and progression. (aacrjournals.org)
Tonsils Thymus Bone Marrow Spleen Lymphoid tissue of the gut The Body's Immune System Balancing the Mediators of the Immune System Proinflammatory and Neurotoxic Factors TH1 and TH17 cytokines TNF IL-1 osteopontin leukotrienes MMP plasminogen activators nitric oxide reactive oxygen species glutamate antibody + complement cell-mediated cytotoxicity neurotrophins via p75NTR? (studyslide.com)
Microglia are immune cells in the CNS, and known to have inflammatory functions via releasing proinflammatory cytokines such as tumor necrosis factor (TNF)-α and interleukin (IL)-1β 6 . (nature.com)
Special considerations are given to the causal involvement of novel molecular pathways, including cytokines, growth factors, and intracellular signaling cascades in RA pathophysiology, and the emerging role of targeted therapeutic agents in the management of RA. (jscimedcentral.com)
and producing cytokines, such as aging procedures such as endoscopic with ~750 000 new cases every year interleukin (IL)-10, that modulate the ultrasonography, which can lead to ( 1 ). (who.int)

An isotype control should be used at the same concentration as the antibody of interest. (bdbiosciences.com)
On the other hand effects of IL-10 are pleiotropic as it stimulates B cell survival proliferation differentiation and antibody isotype switching [6]. (researchassistantresume.com)
Certolizumab pegol (CDP870, tradename Cimzia) is a recombinant, humanized, pegylated monoclonal antibody (mAb) formed with a humanized Fab fragment of 50 kDa, from an IgG 1 isotype, fused to a 40 kDa polyethylene glycol moiety replacing the Fc antibody region. (creativebiolabs.net)

The following product was used in this experiment: FAS Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-110937, RRID AB_2856348. (thermofisher.com)
Immunohistochemical staining of FAS in human rectum and skeletal muscle tissues using FAS Polyclonal Antibody (Product # PA5-110937). (thermofisher.com)

We used gradient-boosted machine learning and lasso regularised Cox regression in a random 75% training subsample to assess whether adding proteins to risk factors included in the Swedish National Diabetes Register risk model would improve the prediction of MACE in the separate 25% test subsample. (springer.com)
The TWEAK Receptor binds TWEAK (CD255) and can signal intracellularly by interactions with TRAF1, 2, and 3 cytoplasmic proteins leading to NF-κB activation. (bdbiosciences.com)
The CD134 antigen, through its interaction with adaptor proteins TRAF2, TRAF3 and TRAF5, is a costimulatory signal transducer of T-cell receptor-mediated activation that has been shown to activate NF-κB, a signaling factor involved in transcription of survival genes. (beckman.com)

The CD134 antigen is a 35 kDa, type-1 transmembrane glycoprotein member of the tumor necrosis factor superfamily (TNFRSF) and is allocated number 4. (beckman.com)

Interestingly, the most primordial defense responses employed by myeloid cells against pathogens, such as complement activation, antibody-dependent cell cytotoxicity and phagocytosis, actually seem to favor cancer progression. (frontiersin.org)
NK cells are specialized effectors of the innate immune system that destroy their targets by antibody-dependent cell-mediated cytotoxicity, have prominent antitumor effects, and are potent killers of virally infected cells. (medscape.com)
Certolizumab pegol differs from the other TNFα-inhibitors in its lack of an Fc region, which minimizes potential Fc-mediated effects such as complement-dependent cytotoxicity (CDC) or antibody dependent cell-mediated cytotoxicity (ADCC). (creativebiolabs.net)

It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule. (exbio.cz)
CD137 is a costimulatory molecule functioning to stimulate T cell proliferation, dendritic cell maturation, and promotion of B cell antibody secretion. (wikipedia.org)
For instance, increases in adhesion molecules, including vascular adhesion molecule-1 or intracellular adhesion molecule-1, on epithelial cells causes recruitment of immune cells like macrophages and neutrophils. (wikipedia.org)
Homo sapiens CD40 molecule (CD40), transcript variant 1, mRNA. (origene.com)

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD95 (LT95) PE-DyLight® 594 antibody (4 μl reagent / 100 μl of peripheral whole blood). (exbio.cz)
Separation of human CD95 positive T cells (red-filled) from CD95 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD95 (LT95) PE-DyLight® 594 antibody (4 μl reagent / 100 μl of peripheral whole blood). (exbio.cz)
This antibody was developed for use in flow cytometry. (bdbiosciences.com)
In parallel in vitro studies, flow cytometry was used to determine the influence of TNF-α on proliferation and integrin expression of human brain microvascular endothelial cells (HBMECs). (biomedcentral.com)

1. Makino T, Noguchi Y, Yoshikawa T, Doi C, Nomura K. Circulating interleukin 6 concentrations and insulin resistance in patients with cancer. (dadamo.com)

The apoptosis was transduced by multiple signaling pathways mediated by FasL and TNFR2 and mainly activated the mitochondrial control of apoptosis via Bid and Bim, which was rescued by silencing Bid or/and Bim . (biomedcentral.com)
BF-rTK + GCV induced tumor apoptosis mediated by FasL and TNFR2 through the mitochondrial control of apoptosis via Bid and Bim without inducing necroptosis and autophagy. (biomedcentral.com)
however, the role of the two distinct TNF-α receptors, TNFR1 and TNFR2, in mediating angiogenic signaling after cerebral ischemic stroke is relatively unknown. (biomedcentral.com)
C57BL6 mice were subject to 90 min of ischemia by temporary occlusion of the middle cerebral artery (MCAO) and given daily intra-cerebroventricular injections of antibodies against TNFR1, TNFR2 or control IgG (doses of 10, 50, and 100 ng/day) for 4 days following 90 min MCAO. (biomedcentral.com)
The post-ischemic cerebral angiogenic response was inhibited by antibodies against TNFR1 but not TNFR2, and this correlated with reduced endothelial proliferation and decreased α5β1 and αVβ3 integrin expression after 4 and 7 days post-ischemia. (biomedcentral.com)
Consistent with these findings, in vitro studies showed that TNF-α induced endothelial proliferation and upregulation of α5β1 and αVβ3 integrins was abrogated by anti-TNFR1 but not anti-TNFR2 antibodies in cultured HBMECs. (biomedcentral.com)
TNF-α interacts with two types of cell surface receptors, TNF receptor 1 (TNFR1) and TNFR2 [ 12 ]. (biomedcentral.com)
TNFR1 is expressed on all cell types, and this is a major signaling receptor for TNF-α, whereas TNFR2 is expressed primarily by endothelial cells and hematopoietic and some neuronal populations and mediates limited biological responses [ 12 ]. (biomedcentral.com)

Inhibitors of tumor necrosis factor alpha (anti-TNFα) have been used to treat irEC. (bmj.com)
Targeting the tumor necrosis factor-alpha (TNFα), it has been approved by the U.S. Food and Drug Administration (FDA) to treat adults with Crohn's disease (CD), rheumatoid arthritis (RA), psoriatic arthritis (PsA), ankylosing spondylitis (AS), non-radiographic axial spondyloarthritis (nr-axSpA), and plaque psoriasis (PsO). (creativebiolabs.net)

TWEAK-induced TWEAK Receptor-mediated signaling can play roles in inflammation, the induction of apoptosis in certain cell types, the proliferation and migration of endothelial cells, and can promote angiogenesis within healthy and diseased tissues, eg, tumors. (bdbiosciences.com)
Binding of complement to a foreign substance, or antigen, amplifies and augments the body's innate immune system by means of its role as an opsonin (a factor that enhances phagocytosis of unwanted particles) and as a chemoattractant (a factor that recruits cells to areas of inflammation). (medscape.com)
These receptors trigger inflammation through the NFkB-dependent and interferon regulatory factor-dependent signaling pathway. (medscape.com)
Furthermore, BF-rTK + GCV showed to repress the inflammation of tumor through downregulating TNF-α expression. (biomedcentral.com)
Figure 1: Relationships among nutrition, inflammation and immunity, and cancer. (nature.com)
These data suggest that ML may contribute to TNF-mediated inflammation and focal demyelination by rendering SCs more sensitive to TNF within the nerves of patients with leprous neuropathy. (bvsalud.org)
IL-6 is one of the important pro-inflammatory factors, especially in early phage of inflammation. (dadamo.com)

This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. (origene.com)
Anti-Siglec-8 antibody administration in vivo to humanized and transgenic mice selectively expressing Siglec-8 on mouse eosinophils and mast cells confirmed the in vitro findings, and identified additional anti-inflammatory effects. (mdpi.com)
The analysis of inflammatory markers (TNF-α) in tumor indicated that BF-rTK + GCV significantly inhibited TNF-α expression. (biomedcentral.com)
We further found that BF-rTK + GCV inhibited the expression of the inflammatory maker of TNF-α. (biomedcentral.com)
The microRNA mir155 has been implicated in mediating downstream pro-inflammatory response to LPS, and it has has also been implicated in the pathogenesis of rheumatoid arthritis (RA). (biomedcentral.com)
Thus, our data suggested that quercetin might exert its anti-inflammatory effect through negatively modulating pro-inflammatory factors, such as IL-6. (dadamo.com)
The American College of Rheumatology (ACR) and the European League Against Rheumatism (EULAR) define RA as a chronic inflammatory disease characterized by joint swelling, joint tenderness, and destruction of synovial joints, leading to severe disability and premature mortality [1]. (jscimedcentral.com)
However, a significant contributing factor is the deceptively poor inflammatory response seen in the absence of antibodies. (medscape.com)
A few of these inflammatory responses, such as inflammatory cell recruitment, can be modulated by alcohol exposure [ 1 , 2 , 3 , 4 ]. (cdc.gov)
Alcohol also interferes with proper inflammatory responses by disturbing the enzymatic processing of TNFα by TNFα Converting Enzyme (TACE) and abrogating TNFα/TACE function [ 11 , 12 ]. (cdc.gov)

Furthermore, HIF-1 actively limits the mitochondrial consumption of pyruvate at two levels: (i) through the enzyme PDK (PDH-kinase), which in turn inhibits PDH activity preventing the conversion of pyruvate into acetyl CoA, and thereby limiting mitochondrial metabolism of pyruvate [ 6 ] and (ii) through the direct activation of LDH [ 7 , 8 ]. (hindawi.com)
In nerve biopsies of 3 patients with neuritis, we identified tumor necrosis factor (TNF), TNF receptors, and TNF-converting enzyme in Schwann cells (SCs) using immunofluorescence. (bvsalud.org)
In contrast, cells harvested from mice with a conditional deletion of the gene encoding the TNF-α-converting enzyme (TACE/ADAM17), a sheddase for multiple EGF-family proligands, displayed a near-complete attenuation of ERK and Akt phosphorylation responses and compressive stress-induced gene regulation. (elsevierpure.com)

Patients and controls were genotyped for the A/C polymorphism at position −863 of the TNF-α gene promoter region. (molvis.org)
Cancer gene therapy approaches include the direct killing of tumor cells by injecting a therapeutic gene into the tumor cell or employing vaccine strategies to deliver an immunomodulatory gene that stimulates the immune system to recognize tumor antigens [ 1 ]. (biomedcentral.com)
Tumour necrosis factor (TNF) receptor- associated periodic syndrome (TRAPS) is a hereditary autoinflammatory condition resulting from a range of mutations in the TNFRSF1A gene. (biomedcentral.com)
This phenomenon often causes overexpression of MDR1 gene that encodes a 170-KD transmembrane glycoprotein named as P-glycoprotein (P-gp, ABCB1) [1]. (ibs-italy.info)
The resultant DNA is placed in a mammalian cell culture, which then expresses the resultant gene, producing the desired antibody. (msdmanuals.com)

The antibody LT95 reacts with an extracellular epitope on CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. (exbio.cz)

Immune cells abundantly infiltrate tumors, creating a complex environment mediated by repetitive cycles of antitumor responses and immune evasion ( 1 ). (frontiersin.org)
Utomilumab (PF-05082566) targets this receptor to stimulate a more intense immune system attack on cancers. (wikipedia.org)
Siglecs (sialic acid-binding immunoglobulin-like lectins) are single-pass cell surface receptors that have inhibitory activities on immune cells. (mdpi.com)
The generation of receptors specific for antigens is a unique and complex process that generates 10 12 specific receptors for each cell type of the adaptive immune system, including T and B cells. (medscape.com)
The microarchitecture of secondary lymphoid organs plays an important role in facilitating the activation and maturation of antigen-specific lymphocytes and ultimately the mounting of an adaptive immune response ( 1 ). (rupress.org)
They were divided into 2 groups: 12 with immune-mediated thrombocytopenia (Group 1) and 12 with non-immune mediated thrombocytopenia (Group 2). (who.int)

We will combine super-resolution microscopy ( d STORM/PALM) [1, 2] with light-sheet microscopy as an ideal tool to investigate cellular responses over time with high spatial resolution. (uni-wuerzburg.de)
Recent animal studies have shown that concurrent treatment with anti-TNFα and ICIs improves tumor responses and decreases colitis severity. (bmj.com)
Our results suggest that TNFR1-mediated signaling plays a critical role in triggering angiogenic integrins and subsequent angiogenic responses following cerebral ischemia. (biomedcentral.com)
However, cells derived from mice with targeted deletions of the EGFR ligands Tgfα and Hb-egf showed only modest decreases in responses, even when combined with neutralizing antibodies to the EGFR ligands epiregulin and amphiregulin, suggesting redundant or compensatory roles for individual EGF family members in mechanotransduction. (elsevierpure.com)
Here we report that when mice lacking the RelA subunit of NF-κB are brought to term by breeding onto a tumor necrosis factor receptor (TNFR)1-deficient background, the mice that are born lack lymph nodes, Peyer's patches, and an organized splenic microarchitecture, and have a profound defect in T cell-dependent antigen responses. (rupress.org)

CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. (exbio.cz)
The specific receptor for CD30L is CD30/TNFRSF8, a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. (rndsystems.com)
FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. (thermofisher.com)

In addition, blocking antibodies to α5β1 and αVβ3 integrins significantly inhibited TNF-α-induced HBMEC proliferation. (biomedcentral.com)

Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. (qedbio.com)
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling TACE with 1131 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green). (qedbio.com)

These peptide usually contains the epitope recognized by the antibody. (affbiotech.com)
In fact IL-10 has been reported to activate B cells to promote autoantibody production like rheumatoid factor or antibodies against cyclic citrullinated peptide [10-12]. (researchassistantresume.com)

To detect rTNF-alpha by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. (abcam.co.jp)

The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. (thermofisher.com)

PRR on myeloid cells can be triggered by pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs), but also indirectly by secondary mechanisms such as complement activation and circulating antibodies (Abs), resulting in cytolytic and phagocytic effector mechanisms. (frontiersin.org)
They recognize microorganisma through their Fc, complement and toll-like receptors (TLR), as well as non-TLR. (medscape.com)
Opsonins are often antibodies or components of the complement pathway that bind to the surface of target organisms to facilitate this phagocytosis. (medscape.com)
Donor-specific antibodies bind with high affinity to the vascular endothelium of graft and activate complement . (wikidoc.org)

The intracellular domain of the p75 neurotrophin receptor (p75ICD) lacks catalytic activity but contains a motif similar to death domains found in the cytoplasmic regions of members of the tumor necrosis factor receptor family and their downstream targets. (embl-heidelberg.de)
An antagonist decoy receptor (designated as TRID for TRAIL receptor without an intracellular domain) that may explain the resistant phenotype of normal tissues was identified. (embl-heidelberg.de)

Hence, many effects of nutrition on the development and progression of cancer lack 'specificity' in their association, and such alimentary cues also affect the risk of developing metabolic syndrome, a prelude to arteriosclerosis and neurodegeneration ( Fig. 1 ). (nature.com)
Specificity This antibody recognizes human, mouse, and rat TACE, and 80-130kDa bands are detected in immunoblots. (qedbio.com)

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)

The ITEM-4 antibody can induce the death of certain TWEAK-sensitive tumor target cell lines. (bdbiosciences.com)

macrophages] in response to pathogen associated molecular patterns (PAMPs) binding the Toll-like Receptor (TLR) present on an active macrophage. (dadamo.com)

An antagonist decoy receptor and a death domain-containing receptor for TRAIL. (embl-heidelberg.de)
Iatrogenic blockade of TNF MHY1485 may reveal proinflammatory effects of its endogeneous antagonist IL-10. (researchassistantresume.com)

CD30 ligation by CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis. (rndsystems.com)
Extending these observations, colon biopsies from E. faecalis -colonized IL-10 −/− mice exhibited crypt hyperplasia and increased staining for macrophages, TNF-α, netrin-1, NF-κB, Tnfrsf1b, and the proliferation marker proliferating cell nuclear antigen while also displaying a reduction in epithelial cell apoptosis. (aacrjournals.org)
In addition, pure TNF-α promoted BEC proliferation in a dose-dependent manner [ 10 ]. (biomedcentral.com)

Drosopoulos KG, Roberts ML, Cermak L, Sasazuki T, Shirasawa S, Andera L, Pintzas A.: Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway. (exbio.cz)

Excessive level of BAFF causes abnormally high antibody production, results in systemic lupus erythematosus, rheumatoid arthritis, and many other autoimmune diseases. (wikidoc.org)
Introduction The introduction of tumor necrosis factor (TNF) blocking brokers into the therapy of rheumatoid arthritis (RA) is a story of remarkable success [1]. (researchassistantresume.com)

In cell cocultures, we observed increased expression of the TNF-α receptor Tnfrsf1b and Netrin-1. (aacrjournals.org)
RNAi-mediated attenuation of Tnfrsf1b decreased TNF-α-induced netrin-1 production and augmented epithelial cell apoptosis in culture. (aacrjournals.org)

TWEAK Receptors are relatively highly expressed on HUVEC cells and certain tumor cell lines. (bdbiosciences.com)
CD30 was originally identified as a cell surface antigen of Hodgkin's and Reed-Sternberg cells using the monoclonal antibody Ki-1. (rndsystems.com)
However, cancer cells overcome these controls, in particular by acquiring genetic mutations leading to the activation of oncogenes (pten, myc) or loss of tumor suppressors (p53) [ 1 ]. (hindawi.com)
In normal cells, the fate of pyruvate depends on many factors, one of which is oxygen availability. (hindawi.com)
In contrast, cancer cells shift their metabolism toward lactate production even in the presence of oxygen [ 4 ], partly through genetic modifications that stabilize the transcription factor Hypoxia Inducible Factor (HIF) involved in the adaptation of the cells to hypoxia, under nonhypoxic conditions as well as generating an adaptive response to the hypoxic microenvironment (Figure 1 ). (hindawi.com)
Pluristem Therapeutics, based in Haifa, Israel, is currently recruiting patients in the United States and Israel for its Phase 1 trial of PLX-R18 cells in this unmet need. (drugdiscoverynews.com)
This antigen was originally named OX40 from the first antibody that led to the discovery of this antigen on activated rat CD4+ T cells. (beckman.com)
Cells were labeled with antibodies against ß-tubulin (green) and Alexa 647 phalloidin (magenta). (uni-wuerzburg.de)
C) Jurkat cells were labeled with an anti-ceramide C16 antibody and a secondary Alexa 647 labeled antibody. (uni-wuerzburg.de)
Although the structure of the granulomas and multinucleated giant cells in granulomatosis with polyangiitis (GPA) is known, little is established about what drove granuloma formation and why it is infrequently found in patients with myeloperoxidase-antineutrophil cytoplasm antibodies. (bmj.com)
BAFF steady-state concentrations depend on B cells and also on the expression of BAFF-binding receptors. (wikidoc.org)
These receptors are expressed mainly on mature B lymphocytes and their expression varies in dependence of B cell maturation (TACI is also found on a subset of T-cells and BCMA on plasma cells ). (wikidoc.org)
Mediates the proteolytic cleavage and shedding of FCGR3A upon NK cell stimulation, a mechanism that allows for increased NK cell motility and detachment from opsonized target cells. (qedbio.com)
In both cases, the process usually begins as above with production of mouse hybridoma cells that make antibody to the desired antigen. (msdmanuals.com)
In the absence of mature B cells, patients lack lymphoid tissue and fail to develop plasma cells, the cells that manufacture antibodies. (medscape.com)
Activation of Btk results in a cascade of signaling events resulting in calcium mobilization and fluxes, cytoskeletal rearrangements, and transcriptional regulation involving nuclear factor-kappaB (NF-kappaB) and nuclear factor of activated T cells (NFAT). (medscape.com)
1) Antibody deficiency disorders are defects in immunoglobulin-producing B cells . (lu.se)
3) T cell deficiencies result usually in combined immunodeficiencies (CIDs), where both T cells and antibody production are defective. (lu.se)

The ITEM-4 antibody reportedly crossreacts strongly with mouse CD266 as demonstrated by flow cytometric analysis and functional assays. (bdbiosciences.com)

Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT) (PubMed:24226769). (qedbio.com)

GLP-1 and incretin mimetics, such as exenatide, have been shown to attenuate hepatocyte steatosis in vivo and in vitro, but the specific underlying mechanism is unclear. (diabetesjournals.org)
Since its discovery in 2000, initial in vitro studies have found that the engagement of Siglec-8 with a monoclonal antibody or with selective polyvalent sialoglycan ligands induced the cell death of eosinophils and inhibited mast cell degranulation. (mdpi.com)

Our data demonstrate that SIRT1 mediates the effect of exenatide on ameliorating hepatic steatosis, suggesting the GLP-1 receptor agonist could serve as a potential drug for nonalcoholic fatty liver disease (NAFLD), especially in type 2 diabetes combined with NAFLD, and SIRT1 could be a therapeutic target of NAFLD. (diabetesjournals.org)
Outcome predictors of biologic therapeutic medications like TNF inhibitors are of interest since side effects like serious infections or malignancy cannot be completely ruled out. (researchassistantresume.com)
One important drug in this field is the anti IL- 6 receptor antibody (MRA), used as one of new therapeutic approaches in rheumatic arthritis. (dadamo.com)

Antibodies: TACE (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. (qedbio.com)
Antibodies: TACE 1131 (0.25 ug/mL) and beta-actin 3779 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. (qedbio.com)
Antibodies: TACE 1131 (0.5 ug/mL), TACE 22-001 (2 ug/mL), and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST. (qedbio.com)

Anatomy25

Organisms11

Diseases7

Chemicals and Drugs130

Analytical, Diagnostic and Therapeutic Techniques and Equipment35

Phenomena and Processes50

Disciplines and Occupations2

Information Science3

Health Care4

Monoclonal antibodies11

Cytokine8

Ligand10

Soluble6

Superfamily member1

Murine6

Protein20

Cytokines9

Isotype3

Polyclonal2

Proteins3

Member of the tumor1

Cytotoxicity3

Molecule4

Flow Cytometry4

Interleukin1

TNFR28

Tumor necro2

Inflammation7

Inflammatory10

Enzyme3

Gene5

Tumour1

Immune6

Responses5

Glycoprotein3

Significantly inhibited1

Rabbit2

Peptide2

ELISA1

Apoptosis mediated1

Complement4

Intracellular2

Specificity2

Affinity1

Induce1

Pathogen1

Antagonist2

Proliferation3

Death receptor1

Rheumatoid arthritis2

Tnfrsf1b2

Cells18

Assays1

Activator1

Vitro2

Therapeutic3

TACE3