AnatomyDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureHealth Care
Immunoradiometric AssayRadioimmunoassayImmunoassayThyrotropinAntigens, Tumor-Associated, CarbohydrateHyperthyroidismReagent Kits, DiagnosticAntibodies, MonoclonalGlycoprotein Hormones, alpha SubunitEthacridineRadioimmunosorbent TestHyperparathyroidismThyroid Function TestsThyrotropin-Releasing HormoneReference ValuesIodine RadioisotopesThyroid DiseasesParathyroid HormoneParathyroid Hormone-Related ProteinHypercalcemiaMicrochemistryHypothyroidismThyroglobulinQuality ControlFluoroimmunoassayProinsulinFerritinsAutoanalysisAdrenocorticotropic HormoneCross ReactionsBiotinCathepsin DChorionic Gonadotropin, beta Subunit, HumanSensitivity and SpecificityEpitopesThyroxineAntigen-Antibody ComplexChorionic GonadotropinAntigens, NeoplasmAntibody SpecificityEvaluation Studies as TopicPeptide FragmentsImmunoglobulin GTriiodothyronineTumor Markers, BiologicalCarcinoembryonic AntigenPregnancyAntibodiesInsulin-Like Growth Factor Binding Protein 3Reproducibility of ResultsElectric ImpedanceSphygmomanometersMuscle, SkeletalBody CompositionBlood Pressure DeterminationMetabolic Syndrome XBody Water
IRMASerumEnzyme-linked immunosorbent assaysRadioimmunoassayParathyroid hormoneImmunologicalConcentrations were measuredSensitivityAntibodiesRadiometricCoefficientImmunometricUrinaryAntibodyIsotopicSandwichImmunofluorescenceQuantitativeHormoneDiffersSpecificCytologyResultsRadioactivityProteinTubesVitaminFunctionalEvaluateCalciumTechniqueLevelsELISAPatientsHumanOBJECTIVEClinicalBloodTotalMetabolicEarlyHeight
IRMA8
  • 12 ] in the early 1970s with a two-site immunoradiometric assays (IRMA) for ferritin. (lww.com)
  • It was later described when hormones were being oversecreted and measured using IRMA. (lww.com)
  • Ferritin, folic acid and TSH were measured in serum by immunoradiometric assay (IRMA), charcoal boil and double antibody assay kit (DPC, Los Angeles, California), respectively. (who.int)
  • Immunoradiometric assay (IRMA) is an assay that uses radiolabeled antibodies. (wisdomanswer.com)
  • However, as the ELISA is a solid-phase assay the detection limit is not as good as the fluid-phase IRMA (presumably due to steric hindrance). (wisdomanswer.com)
  • Usually, hGH is quantified by an immunoradiometric assay (IRMA), resulting in a linear range of approximately 0.1 to 50ng hGH/ml. (thomassci.com)
  • Methods: We used our home made radioiodine (125I)-based IRMA kit for the detection of 25(OH)D. It is based upon, non-competitive displacement agents which enable effective separation of vitamin D metabolites from binding proteins to enable the amount of vitamin D metabolites to be measured, without competing with the proteins or requiring its solvent extraction from the serum [2,3]. (longdom.org)
  • Microparticle enzyme immunoassay (MEIA) for thyrotropin (TSH) was evaluated and results were compared with those of immunoradiometric assay (IRMA) procedure. (e-emj.org)
Serum12
  • The diagnostic program should be performed within hours, in parallel with measures to lower serum calcium. (wikipedia.org)
  • 25-OHD (25-hydroxyvitamin D), the major circulating metabolite of vitamin D, was measured in serum by competitive protein-binding (CPB) assay and radioimmunoassay kit (Nichols, San Juan Capistrano, California). (who.int)
  • Serum and intratesticular hormones were measured by immunoradiometric assays. (bvsalud.org)
  • In LASA, serum osteocalcin (OC) and deoxipyridinolin (DPD/Cr) were measured as bone markers. (lasa-vu.nl)
  • Serum levels of intact OC were measured using a immunoradiometric assay (Biosource Diagnostics, Fleurus, Belgium). (lasa-vu.nl)
  • Fasting serum FGF21 and adiponectin levels as well as fasting insulin, glucose, transaminases, and lipid profiles were measured by enzyme-linked immunosorbent assays in 45 lean and 70 obese children aged 7-14 years. (jomes.org)
  • However, previous studies used various cutoff values of serum SCC-Ag, ranging from 1.5 to 3.0 ng/mL, which was determined to correspond to the 95th, 98th, and 99th percentiles, or 2 times the 95th percentile of serum concentrations measured in a population of healthy women [ 6 8 10 11 12 13 14 15 16 17 18 ]. (koreamed.org)
  • Serum IGF-I and IGFBP-3 were measured via an immunoradiometric assay using a DSL kit (Diagnostic Systems Laboratories). (e-enm.org)
  • Measuring serum IGF-I and IGFBP-3 is essential for diagnosis and monitoring of treatment responses in GH deficiency such as hypopituitarism, growth hormone-releasing hormone (GHRH) receptor mutation, or GH insensitivity [ 8 ] and GH excess such as acromegaly or gigantism [ 9 ]. (e-enm.org)
  • Serum placental GH concentrations were measured by a highly specific immunoradiometric assay, and fetal size was measured by ultrasound. (regsj.dk)
  • The development of a solid-phase immunoradiometric assay to satisfy the pressing need for a simple yet effective method for measuring 25-hydroxyvitamin D[25(OH)D] in a serum and thus more suited to routine use in clinical biochemical laboratories[1]. (longdom.org)
  • Serum T4, T3 and TSH were measured using RIA kits. (irispublishers.com)
Enzyme-linked immunosorbent assays1
  • 25 ] In India, the laboratory methods commonly followed are immunometric and enzyme-linked immunosorbent assays where the prevalence of hook effect is more. (lww.com)
Radioimmunoassay3
Parathyroid hormone7
Immunological2
  • High-dose effect is a common phenomenon documented in various immunological and serological assays, which results in false-negative results. (lww.com)
  • Immunological factor VII assays can be used to measure factor VII:Ag (antigen levels). (medscape.com)
Concentrations were measured1
Sensitivity5
  • In conclusion, CA 549 assay has an acceptable sensitivity with high specificity. (nih.gov)
  • Using the standard isotopic protocol, secretion of hGH protein into the culture-medium supernatant can be monitored approximately 24hours post-transfection, depending on the cell type and the sensitivity of the assay used. (thomassci.com)
  • The sensitivity of the assay (mean±2 SD of the zero standard) was 0,0158 µIU/ml. (e-emj.org)
  • achieved a 72 % sensitivity rate for the BTA TRAK assay in individuals with recurrent bladder cancer. (icliniq.com)
  • Studies have shown a specificity and a sensitivity rate of 75 % and 66 %, respectively, for the NMP22 assay. (icliniq.com)
Antibodies6
  • But, in all sandwich assays, the signal begins to plateau with high concentrations of analyte due to limiting amounts of reagent antibodies and rare samples with extremely high concentrations of analyte can even lead to the hook effect in assays with a wash step. (lww.com)
  • This is an enzyme-linked immunosorbent assay using specific antibodies or soluble mutant tissue factor that is insensitive to native factor VII but that serves as a cofactor for factor VIIa catalyzed activation of factor X. (medscape.com)
  • Immunometric assays, also known as sandwich ELISAs, use two antibodies specific to the antigen to capture or "sandwich" antigens in the well for detection. (wisdomanswer.com)
  • The sandwich ELISA is a type of Enzyme-linked immunosorbent Assay that uses two antibodies: a capture antibody and a detection antibody. (wisdomanswer.com)
  • Thus, a specific hCG assay is only possible by means of antibodies directed against the β-subunit. (diakey.com)
  • To measure levels of T3, T4 and TSH known amount of radioactively labeled antigen mixed with fixed amount of specific antibody and anti-immunoglobulin antibodies. (irispublishers.com)
Radiometric1
  • 15 g/L) are derived from expert opinion based on radiometric assays in use decades ago. (cdc.gov)
Coefficient2
  • To estimate the coefficient of variation (CV), the measurements were measured twice in a subsample. (lasa-vu.nl)
  • Intra- and inter-assay coefficient of variation of OC. (lasa-vu.nl)
Immunometric4
  • Theoretically, this issue is only applicable to sandwich immunometric assays without a wash step between reagent additions. (lww.com)
  • High-dose hook effect still remains a problem in many assay techniques like one-step immunometric assays, [ 2 3 4 5 6 7 8 9 ] immunoturbidimetric assays, [ 10 ] and immunonephelometric assays. (lww.com)
  • What is immunometric assay? (wisdomanswer.com)
  • Immunometric assays exhibit a direct correlation between antigen concentration and substrate response. (wisdomanswer.com)
Urinary1
  • Urinary excretions of DPD were measured by competitive immunoassay (ACS 180, Chiron Diagnostics, Emeryville, USA) Values were corrected for creatinine concentration in the same urine sample. (lasa-vu.nl)
Antibody1
  • The aim of this study was not to compare our home made with commercially available method but tested for general assay performance, including homemade polyclonal antibody specificity. (longdom.org)
Isotopic1
  • Performance characteristics of the radio isotopic method for the hormone assay were identified. (irispublishers.com)
Sandwich1
  • Circulating concentrations of growth-differentiation factor 15 in apparently healthy elderly individuals and patients with chronic heart failure as assessed by a new immunoradiometric sandwich assay. (biovendor.com)
Immunofluorescence1
  • It is performed along with voided urine cytology and is an immunocytology immunofluorescence assay. (icliniq.com)
Quantitative1
  • BTA TRAK is a quantitative assay, and BTA stat is a quantitative test. (icliniq.com)
Hormone1
  • Plasma PTH profiles were subjected to deconvolution analysis, which resolves measured hormone levels into secretion and clearance components. (elsevierpure.com)
Differs1
  • The hGH assay system differs from most of the other commonly used reporter proteins (CAT, β-Gal, or luciferase) in one important respect: hGH is a secreted protein, and is measured using samples of culture-medium supernatants thus (i) avoiding the necessity to lyse cells, (ii) allowing continuous monitoring of transient expression kinetics, and (iii) allowing use of the cells for other purposes (e.g. (thomassci.com)
Specific1
  • A specific assay for factor VII, using known factor VII-deficient plasma, is required to confirm the diagnosis. (medscape.com)
Cytology1
  • The addition of any one tumor marker assay consistently improved the diagnostic value of cytology. (nih.gov)
Results4
  • More than 60 years ago, the phenomenon of high-dose hook effect had been documented in serological assays, which resulted in false-negative results. (lww.com)
  • Past estimates of exposure to methyl mercury has been obtained from results of food consumption surveys and measures of methyl mercury in fish. (cdc.gov)
  • Results can vary dramatically depending on the source of tissue factor used in the in vitro assay. (medscape.com)
  • IGFBP-1 was the most abundant IGFBP in CM, and densitometric analysis revealed a 2.5-fold increase in IGFBP-1 under hypoxic, compared with normoxic, conditions, supporting the immunoradiometric assay results. (ox.ac.uk)
Radioactivity2
  • The radioactivity in the tubes is measured in a gamma counter. (diakey.com)
  • The radioactivity was measured from the precipitation. (irispublishers.com)
Protein3
  • IGFBP-1 protein in conditioned medium (CM) was measured by immunoradiometric assay and increased 3- to 4-fold under hypoxic (2% O2), compared with normoxic, conditions. (ox.ac.uk)
  • It is used to measure the human complement factor of H-related protein (hcfhrp) in urine. (icliniq.com)
  • It is used to measure nuclear matrix protein 22, which is released into urine by apoptotic tumor cells. (icliniq.com)
Tubes2
  • If large quantity of assay would be performed at one time, there might be substantial time variation between 60 tubes at one time to minimize time variation. (diakey.com)
  • But due to very large number of assay tubes sometimes end users reduce the counting time to 30 seconds. (irispublishers.com)
Vitamin1
  • Conclusion: Simplification of the methods for extraction and separation has been a key feature in the improvement of assays for vitamin D in Democratic Republic of Congo. (longdom.org)
Functional1
  • Finally, to address the functional role of this cytokine, we measured the effect of human recombinant (hr)IL-1β on seizure activity as one marker of the response to kainate. (jneurosci.org)
Evaluate2
  • A factor VIIa:c assay is used to evaluate therapeutic factor VIIa levels. (medscape.com)
  • Standardization of hepatitis E virus (HEV) nucleic acid amplification technique-based assays: an initial study to evaluate a panel of HEV strains and investigate laboratory performance. (jctlm.org)
Calcium1
  • Chez les femmes préménopausées et postménopausées, la concentration sérique en 25-OHD, en phosphore et en calcium était stable dans la fourchette d'âge. (who.int)
Technique1
  • An improved air sample processing technique and an ultrasensitive, two site immunoradiometric assay were developed for measuring papain (9001734) in personal breathing zone samples by using conventional industrial hygiene air sampling equipment. (cdc.gov)
Levels5
  • The levels of the tumor markers were measured by immunoradiometric assays (RIA). (nih.gov)
  • To determine the severity, factor VII activity levels (factor VII:c) are measured using a single-stage, PT-based assay. (medscape.com)
  • We measured the levels of various molecular forms of AM, i.e. an active form of mature AM (AM-m), an intermediate inactive form of glycine-extended AM (AM-Gly) and total AM (AM-T = AM-m+AM-Gly), in plasma and pericardial fluid using our newly developed immunoradiometric assay in consecutive 67 patients undergoing coronary artery bypass graft surgery. (silverchair.com)
  • Pericardial fluid and plasma cAMP, atrial natriuretic peptide and brain natriuretic peptide levels were also measured. (silverchair.com)
  • Bound T3, Bound T4, TSH and Free T4 were assayed using different counting time and two different pulse height window levels. (irispublishers.com)
ELISA2
  • Why is the ELISA used instead of an immunoradiometric assay? (wisdomanswer.com)
  • The hGH ELISA is used to quantitatively measure the expression level of hGH released into cell culture supernatant of eukaryotic cells transfected with a plasmid containing a reporter gene that encodes for hGH. (thomassci.com)
Patients1
  • Immunoradiometric assay some research patients with rare percent less than vials. (queobathrooms.co.uk)
Human1
  • Commutability of the certified reference materials for the standardization of beta-amyloid 1-42 assay in human cerebrospinal fluid: lessons for tau and beta-amyloid 1-40 measurements. (jctlm.org)
OBJECTIVE1
  • OBJECTIVE: We examined relationships of SF measured using an immunoradiometric assay from the era of expert opinion with 2 independently measured indicators of ID, hemoglobin (Hb) and erythrocyte zinc protoporphyrin (eZnPP), using data from the Third National Health and Nutrition Examination Survey (NHANES III, 1988-1994). (cdc.gov)
Clinical1
  • MAIN OUTCOME MEASURES: Biannual clinical pubertal staging including orchidometry. (bvsalud.org)
Blood2
  • Blood measures of total and inorganic mercury will be important for evaluation of exposure from exposure to mercury in interior latex paints. (cdc.gov)
  • The rate of bone turnover can be assessed by measuring biochemical markers of bone formation and bone resorption in the blood or urine (see for review 5,6). (lasa-vu.nl)
Total1
  • The factor VII:c assay uses the ability of tissue factor to promote the conversion of factor VII to factor VIIa and measures the total clotting activity of factor VII and factor VIIa. (medscape.com)
Metabolic2
  • 1 , 2 This has attracted interest to the use of biomarkers to identify persons who are at risk for the development of metabolic abnormalities and for whom preventive measures could be tailored. (jomes.org)
  • This could yield highly favorable changes in circulating anthropometric variables adiponectin and, as a result, to the metabolic and cardio- Height of the barefoot subjects was measured to the nearest 0.1 cm. vascular risk. (hmamedicalclinic.com)
Early1
  • In contrast, gonadarche and pubarche are early and clinically well-defined measures of puberty onset. (bvsalud.org)
Height1
  • And an aortic aneurysm measuring height for a child and meals and any of its excipients. (queobathrooms.co.uk)