Mass peptide mapping. Medical search. Frequent questions

This is achieved by using computer programs that translate the known genome of the organism into proteins, then theoretically cut the proteins into peptides, and calculate the absolute masses of the peptides from each protein. (wikipedia.org)
Due to the long, tedious process of analyzing proteins, peptide mass fingerprinting was developed. (wikipedia.org)
The portable size ZipChip Interface directly mounts onto select models of Thermo Scientific mass spectrometers, and creates a seamless CE/ESI-MS workflow that offers fast CE separation, nano-spray level sensitivity, and HRAM spectrometry for the characterization of intact proteins, antibody drug conjugates (ADCs), antibody subunits, peptides, and metabolites. (thermofisher.com)
Additionally, these reactions simultaneously react on several post-translationally modified groups in peptides or proteins. (nih.gov)
In support of biotherapeutics across the board, KBI's Mass Spectrometry Core Facility has championed the characterization and management of proteins, active pharmaceutical ingredients (APIs), and impurities through identification, characterization, assessment, and quantitation. (kbibiopharma.com)
Combining immunopurification and subsequent analytical mass spectrometry, hundreds of proteins, including synaptic vesicle proteins, components of the presynaptic fusion and retrieval machinery, proteins involved in intracellular and extracellular signaling and a large variety of adhesion molecules, were identified. (mdpi.com)
However, detecting PTMs on proteins and peptides by MS is challenging because of their low abundance and heterogeneity. (umass.edu)
This dissertation describes the use of supramolecular nanoassemblies, formed by amphiphilic polymers, as novel enrichment tools for the detection and analysis of peptides and proteins that are phosphorylated and glycosylated. (umass.edu)
The multi-attribute method (MAM) is a liquid chromatography-mass spectrometry (LC-MS) based peptide mapping approach used for the identification and quantitation of product quality attributes (PQAs) in therapeutic proteins. (fda.gov)
The identities of the peptides attributed to selected gluten proteins were confirmed using MS/MS with BioMultiView to match the predicted and measured partial amino acid sequences. (unboundmedicine.com)
TY - JOUR T1 - Characterization of wheat gluten proteins by HPLC and MALDI TOF mass spectrometry. (unboundmedicine.com)
In addition to the raw mass spectrometry-based data files, computational processing is performed to map spectra to peptide sequences and identify proteins. (cancer.gov)
Trypsin, MS approved is especially designed for digestion of proteins prior to mass spectrometry analysis. (serva.de)
This technique has frequently been applied to animal tissue slices for the purpose to map proteins, peptides , lipids, sugars or small metabolites to find disease specific bio-markers or to study drag metabolism. (go.jp)
A recent review by the FDA states that the use of mass spectrometry (MS) technologies detailing the characterization of biotherapeutic proteins in regulatory submissions has continued to grow and diversify over the last decade (1). (chromatographyonline.com)
Rapid Disulfide Mapping in Peptides and Proteins by meta -Chloroperoxybenzoic Acid ( m CPBA) Oxidation and Tandem Mass Spectrometry. (bvsalud.org)
Proteins highlighted in red are identified by peptide mass fingerprinting (PMF) and/or by fragment ion analysis. (lu.se)
Consequently, a lot of effort is put into identifying the constituent proteins using mass spectrometric methods. (lu.se)
Several proteins were identified by electrospray ionization (ESI) mass spectrometric analysis and four recombinant protein constructs were expressed and used in a multiplex bead assay (MBA) to detect B. duncani-specific antibodies. (cdc.gov)
Cell proteins were analysed with 2-dimensional electrophoresis and differentially expressed proteins were identified by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. (who.int)
Image analysis of the matched maps identified 7 proteins that were either over- or down-expressed: activated protein kinase c receptor (LACK), alpha tubulin (×2), prostaglandin f2-alpha synthase, protein disulfide isomerase, vesicular transport protein and a hypothetical protein. (who.int)
Collagen peptides are made by breaking down whole collagen proteins into smaller pieces. (medlineplus.gov)
Water 2H and 17O spin relaxation is used to study water dynamics in the hydration layers of two small peptides, two globular proteins and in living cells of two microorganisms. (lu.se)

In this work, we utilized a liquid chromatography-mass spectrometry (LC/MS)-based approach to identify the Asp isomerization in the complementarity-determining regions of a therapeutic monoclonal antibody. (pda.org)
The improvements in both capabilities and robustness of liquid chromatography-mass spectrometry (LC-MS) technology for biopharmaceutical analysis has led to its expanding role beyond product characterization in early development, to the monitoring of product and process attributes in late development, manufacturing, and quality control (QC) (2). (chromatographyonline.com)
SF samples from end-stage knee osteoarthritis patients undergoing total knee replacement surgery and controls, that is, deceased donors without known knee disease were previously run using liquid chromatography mass spectrometry (LC-MS). This data was used to perform new database searches generating results for non-tryptic and semi-tryptic peptides for studies of degradomics in OA. (lu.se)

The methods applied for PTM analysis are most often based on mass spectrometry (MS). In the past few years, considerable progress has been made in developing MS-based proteomics technologies for global PTM analysis. (umass.edu)
Due to its highly specific cleavage of peptides Glu-C is used in proteomics for peptide mapping and protein sequence work. (serva.de)
A next-generation, high-resolution mass spectrometer is designed to give proteomics, metabolomics, biopharmaceutical characterization, and small-molecule scientists the analytical performance required for research and high-throughput analyses. (selectscience.net)
In proteomics, high throughput approaches using mass spectrometry have be- come widely used. (lu.se)
The need to keep track of protein modifications is readily recognized by the proteomics community, and few repositories of known peptide modifications have been created. (lu.se)

Structural elucidation of isocyanate-peptide adducts using tandem mass spectrometry. (cdc.gov)

In this webinar, the benefits of integrating cutting-edge microfluidic technology with capillary electrophoresis (CE) and electrospray ionization (ESI) for enhanced mass spectrometry (MS) analysis is presented. (thermofisher.com)

An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP). (sigmaaldrich.com)
Peptide maps of tryptic digests of HPLC fractions were obtained from a tandem quadrupole time-of-flight mass spectrometer (MALDI QqTOF MS) and were submitted to the ProFound search engine. (unboundmedicine.com)
Here, we provide an optimized method for tryptic digestion and peptide mapping using adalimumab as an example. (sigmaaldrich.com)

Three chemical specific cleavage reactions, one for the carboxyl side of aspartyl peptide bonds, one for the carboxyl side of asparaginyl peptide bonds and another for the amino side of seryl/threonyl peptide bonds have been recently established. (nih.gov)
The presence of proline residues on the carboxy side of the peptide bond inhibits the cleavage. (serva.de)
The presence of a particular BoNT serotype is demonstrated by mass spectrometric detection of the peptide cleavage products corresponding to their specific toxin-dependent location. (cdc.gov)

Integrate capillary electrophoresis (CE) and electrospray ionization (ESI) into a single microfluidic device to rapidly prepare, separate, and electrospray biological samples directly into your Thermo Scientific mass spectrometer . (thermofisher.com)
The ability to couple mass spectrometry to other separation techniques such as capillary electrophoresis, GC, and HPLC makes it a versatile analytical tool for the simultaneous separation and identification of analytes. (sigmaaldrich.com)
Molecular measurements using capillary electrophoresis time-of-flight or orbitrap mass spectrometer platforms revealed proteomic and metabolomic differences between the X. laevis cells that correlated with phenotype. (njacs.org)
Capillary electrophoresis mass spectrometry expands the molecular toolbox of cell biology and neuroscience. (njacs.org)
Here, capillary electrophoresis (CE) coupled to electrospray ionization mass spectrometry (ESI-MS) is implemented to analyze complex mixtures of InsPs and PP-InsPs with high sensitivity. (biomed.news)

Ceftobiprole medocaril Mass Spectroscopy Era and Evaluation of Pin1 pS138 Antibody. (bioskinrevive.com)
Phosphorylated Pin1 S138 peptides had been used to create Pin1 pS138 antibody in rabbit ( SI Components and Strategies ). (bioskinrevive.com)
To study the level of glycation on antibodies, the authors used both intact mass of the reduced antibody and peptide mapping to find the +162 Da mass shift indicating an addition of a hexose sugar. (genovis.com)
The Fc-glycan of an antibody contain 0, 1 or 2 galactose sugars that also gives a mass shift of 162 Da. (genovis.com)
SARS Antibody Test for Serosurveillance a standardized and rapid peptide-based SARS ELISA is characterized for sensitivity and specificity. (cdc.gov)

Phosphorylated Pin1 protein had been digested with trypsin and peptides had been examined by 2D electrophoresis as referred to (30) ( SI Components and Strategies ). (bioskinrevive.com)
A highly specific protease, trypsin, is used to cleave the protein into suitably sized peptides, although a combination of multiple proteases may also be used to generate more complete PTM site information. (sigmaaldrich.com)
The filter membrane retains the mAb of interest until the digestion procedure is complete, following which peptides can pass through the filter while excluding trypsin. (sigmaaldrich.com)

Try our easy to use calculator which will determine the mass of an amino acid sequence. (peptidesynthetics.co.uk)

The peptide masses are compared to either a database containing known protein sequences or even the genome. (wikipedia.org)
Reverse transcription-polymerase chain reac- peptides from the spike, membrane, and nucleocapsid pro- tein sequences of SARS-associated coronavirus. (cdc.gov)
The method's focus is to globally detect mass differences, not to assign peptide sequences or modifications to individual spectra. (lu.se)
The goal is to assign acquired spectra to known peptide sequences and potential co- and post-translational modifica- tions. (lu.se)

A complete workflow for the intact and middle-up mass analysis of reduced and non-reduced monoclonal antibodies based on SEC-MS with sample preparation by protein-A affinity clean-up. (sigmaaldrich.com)
The mass spectrometer was recognized for its ability to offer high resolution accurate mass (HRAM) data for intact analysis of monoclonal antibodies, oligonucleotide mass determination and peptide mapping in drug discovery applications. (biospace.com)
A new study from Janssen by Mo et al, demonstrates the use of intact mass spectrometry to determine the levels of glycation on therapeutic antibodies. (genovis.com)
Using this enzymatic pretreatment, the authors could determine glycation levels using intact mass spectrometry. (genovis.com)

The peptides are then dissolved in a small amount of distilled water or further concentrated and purified and are ready for mass spectrometric analysis. (wikipedia.org)
Today, mass spectrometry is a well-established detection method that offers a multitude of benefits, such as selectivity, sensitivity, and multi-sample analysis. (sigmaaldrich.com)
In addition motif redundancy can affect quantitative and statistical analysis and prevent a realistic comparison of peptide numbers between datasets. (biorxiv.org)
However, motif redundancy among detected peptides by mass spectrometry (MS) leads to errors and ambiguities in the analysis. (biorxiv.org)
Novel mass spectrometric peptide sequencing and analysis technologies allow for modification site mapping at molecular level. (umass.edu)
Every lot is approved for use in in-gel digestion and mass spectrometry analysis. (serva.de)
Endoproteinase Glu-C, recombinant (V8) is a serine endoproteinase used with in-gel digestion and mass spectrometric analysis. (serva.de)
Lysyl Endopeptidase ® is especially designed for use with in-gel digestion and mass spectrometric analysis. (serva.de)
cleaves specifically the peptide bonds at the carboxy-terminal side of Lysine residues and S-aminoethylcysteine residues with a high degree of specificity, making it a valuable tool for protein sequence analysis and for proteome research. (serva.de)
The use of a high-resolution mass spectrometry-based approach for the characterization is commonly referred to as a multi-attribute method (MAM) 1 , as the peptide mapping analysis yields multiple pieces of information about the protein in a single analysis. (sigmaaldrich.com)
To determine the suitability of an instrument for RPLC-MS analysis, a mixture of 14 isotopically labeled peptides is analyzed as a system suitability test prior to each run. (sigmaaldrich.com)
The main advantage of using such internal standards is the tracking and accounting of every step of the sample preparation and analysis procedure by the heavy mass label. (sigmaaldrich.com)
Both maintenance- and set up-free, EASY-IC technique ensures complete confidence with sub-ppm mass accuracy day after day, allowing smaller extraction windows or tolerances to be used for your quantitative and qualitative data analysis, streamlining data decisions - especially when combined with the exceptional resolving power of the Orbitrap Exploris 240 MS. (thermofisher.com)
Furthermore, in this chapter, we provide a protocol for mitochondrial enrichments from Drosophila larvae and flies together with a procedure to generate high-quality peptides for further analysis by mass spectrometry. (biomed.news)
Mass [10] together with information on modifications induced by sample prepa- ration procedures for mass spectrometric analysis, but mass changes are only given as integer values. (lu.se)
Abt Associates-a global company that conducts research, consulting, and technical services in health, environmental and social policy, technology, and international development-commits to pursuing opportunities within new and existing global projects to conduct antibiotic resistance mapping, landscaping, and trend analysis. (cdc.gov)

Dwayne Schrunk, laboratory manager, analytical chemistry services, Iowa State University, said, "The Orbitrap Exploris 120 mass spectrometer allows us to react to emergency testing situations in a variety of matrices without having to do a lot of method development. (technologynetworks.com)
Peptide mass fingerprinting (PMF) (also known as protein fingerprinting) is an analytical technique for protein identification in which the unknown protein of interest is first cleaved into smaller peptides, whose absolute masses can be accurately measured with a mass spectrometer such as MALDI-TOF or ESI-TOF. (wikipedia.org)
Mass spectrometry (MS) is an analytical technique that uses mass-to-charge ( m/z ) ratio to identify compounds in a sample. (sigmaaldrich.com)
Mass spectrometry is among the most powerful analytical techniques available for protein characterization. (kbibiopharma.com)
High-resolution mass spectrometry is a powerful tool used at KBI Biopharma for analytical strategies concerning complex samples. (kbibiopharma.com)
We recently sat down with Michael J. Nold, Ph.D., Senior Director of KBI's Mass Spectrometry Core Facility in Durham, North Carolina, to discuss how advanced Mass Spectrometry plays a significant analytical role in the characterization and quantitation of APIs and impurities in complex biotherapeutics. (kbibiopharma.com)
Advanced analytical capabilities, including mass spectrometry, are critical for the characterization of active pharmaceutical ingredients (APIs) and managing a diverse population of impurities. (kbibiopharma.com)
She has 10 years of experience assessing product quality of biological products including biosimilars and combination products characterized by various analytical methods including mass spectrometry. (fda.gov)
The Thermo Scientific Orbitrap Exploris MX Mass Detector was also a recipient of an R&D 100 Award in the Analytical/Test category . (biospace.com)
Two analytical workflows, subunit multi-attribute monitoring (MAM) and peptide MAM, have emerged to dominate this discussion, and this article is intended to reflect on the active debates over the needs, challenges, and practical limitations for adopting MS-based attribute monitoring for late-development and QC. (chromatographyonline.com)
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers. (lookformedical.com)

Following digestion, peptides and glycopeptides are separated and analyzed by RP-LC-MS.M. (sigmaaldrich.com)

The new Thermo Scientific Orbitrap Exploris 120 mass spectrometer delivers demonstrated qualitative and quantitative capabilities synonymous with Orbitrap high-resolution accurate-mass spectrometry (HRAM), that supports consistent data quality and decision making. (technologynetworks.com)
Deliver on your objectives with the Thermo Scientific Orbitrap Exploris 240 mass spectrometer by combining application flexibility with the performance to drive discovery and identification, plus receive the quantitative precision and accuracy you need to confidently scale up and achieve impact. (thermofisher.com)
Robust ion trapping for MS scans and higher energy collisional dissociation (HCD) MS/MS within the Ion-routing multipole (IRM) combines with reliable Automatic Gain Control (AGC) measurements for controlled injection of the number of ions into the fast-scanning, high-field Orbitrap mass analyzer for premium workhorse quantitative and qualitative performance. (thermofisher.com)
A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds. (lookformedical.com)

Polymorphisms that slightly vary native peptides or inflammatory processes set the stage for abnormal protein folding and amyloid fibril deposition. (medscape.com)
The structure of the oligomer with the S/T-rich region is a large step forward in the understanding of the function of DNAJB6 and how it can bind aggregation-prone peptides and prevent amyloid diseases. (lu.se)

In This Edition Modern & Practical Applications - Accelerating ADC Development with Mass Spectrometry - Implementing High-Resolution Ion Mobility into Peptide Mapping Workflows Chromatogr. (chromatographytoday.com)

Coupling ESI with capillary LC can separate peptides from protein digests, while obtaining their molecular masses at the same time. (wikipedia.org)

The Orbitrap Exploris MX Mass Detector is a part of a multi-attribute method (MAM) workflow that is designed for efficient, scalable production, to enable drug safety and to accelerate drug approval so that new drugs become available to patients faster. (biospace.com)

Toluene diisocyanate (TDI)-specific monoclonal antibodies: production and epitope mapping. (cdc.gov)

Signal/cutoff (S/C) dis- tribution of Center for Disease Control (Taiwan) SARS (severe peptide antigens as the solid-phase immunosorbent. (cdc.gov)

She received a B.A. in Chemistry from Grinnell College and a Ph.D. in Chemistry from the Johns Hopkins University, where she used small molecules and synthetic peptide models to understand the active sites and reaction mechanisms of different metalloenzymes. (fda.gov)
This approach first identifies and locates a product-related impurity (a critical quality attribute) caused by isomerization, deamidation, oxidation, or other post-translational modifications, and then utilizes synthetic peptides and MS to assist the development of a LC-UV-based chromatographic method that separates and quantifies the product-related impurities by UV peaks. (pda.org)
The are not ideal tools for use in retrospective mass assay was developed by epitope mapping, using synthetic screening. (cdc.gov)

The digested protein can be analyzed with different types of mass spectrometers such as ESI-TOF or MALDI-TOF. (wikipedia.org)
With source architecture shared across all next-generation mass spectrometers, accessing the improvements from the Thermo Scientific Orbitrap Tribrid platform enables increased sensitivity, robustness, minimal optimization and maintenance, and the ability to rapidly switch between sources. (thermofisher.com)

Thermo Fisher Scientific's newest Orbitrap high-resolution mass spectrometer is designed to deliver proven measurement capabilities in a system developed for increased productivity. (technologynetworks.com)

The new system extends the Thermo Scientific Orbitrap Exploris portfolio of high-resolution accurate mass systems, which is now comprised of the Thermo Scientific Orbitrap Exploris 480 mass spectrometer, launched in 2019, and the newly introduced Thermo Scientific Orbitrap Exploris 240 and Thermo Scientific Orbitrap Exploris 120 mass spectrometer, allowing users to easily transfer knowledge and methods from research and discovery to routine testing. (technologynetworks.com)
In combination with the Thermo Scientific Vanquish HPLC and UHPLC liquid chromatography systems, and Thermo Scientific ion chromatography systems, the Orbitrap Exploris 120 mass spectrometer offers market-leading technology in a system that provides performance and speed. (technologynetworks.com)
With Thermo Scientific Compound Discoverer 3.2 software, small-molecule researchers are supported through extensive characterization capabilities, access to multiple mass spectral fragmentation library and structural database sources. (technologynetworks.com)
Access to the large Thermo Scientific mzCloud mass spectral fragmentation library enhances connectivity and characterization for small molecule applications to help identify compounds. (technologynetworks.com)
The Thermo Scientific Orbitrap Exploris 240 mass spectrometer expands the proven Orbitrap Exploris platform and delivers mass accuracy, sensitivity and resolving power across a wide dynamic range. (selectscience.net)
Meet the Orbitrap Exploris 240 mass spectrometer: leading performance, application versatility and intelligent data acquisition enable exceptional data quality that is synonymous with Thermo Scientific Orbitrap technology. (thermofisher.com)
Delivering actionable insights faster than ever before by combining proven benefits of Thermo Scientific Orbitrap technology, such as sub 1-ppm mass accuracy and high resolution, with intelligent data acquisition and processing. (thermofisher.com)
We used feedback from customers and service engineers on the Thermo Scientific Orbitrap Exploris 480 mass spectrometer to design a new system from the ground up to deliver the improved reliability and the exceptional performance you expect from Orbitrap mass analyzer technology. (thermofisher.com)
Set-it and forget-it, with at least five days of maximum mass accuracy through the built-in Thermo Scientific EASY-IC™ (internal calibration) ion source. (thermofisher.com)

this was followed by MALDI mass spectrometry (MS), which showed the presence of six or fewer resolved protein components above 20 kDa in each RP-HPLC fraction, giving a total of 93 MS resolved peaks. (unboundmedicine.com)

The matrix molecules are required for the desorption of the peptide molecules. (wikipedia.org)
Matrix and peptide molecules co-crystallize on the MALDI target and are ready to be analyzed. (wikipedia.org)
The target is inserted into the vacuum chamber of the mass spectrometer and the desorption and ionisation of the polypeptide fragments is initiated by a pulsed laser beam which transfers high amounts of energy into the matrix molecules. (wikipedia.org)
The energy transfer is sufficient to promote the ionisation and transition of matrix molecules and peptides from the solid phase into the gas phase. (wikipedia.org)
A mass spectrometer works by converting individual molecules into ions and then analyzing the relative abundance of the generated ions. (sigmaaldrich.com)
MALDI imaging mass spectrometry (imaging MS) has been a powerful tool to map the spatial distribution of molecules on the surface of biological materials. (go.jp)

The Orbitrap Exploris 120 mass spectrometer was designed specifically for laboratories to safeguard their current assays and enhance their provision of high-throughput and differentiated services, while simultaneously minimizing required training and method development times. (technologynetworks.com)
Performance of the Orbitrap Exploris 120 mass spectrometer is further enhanced with compatibility to Thermo Fisher's latest software updates. (technologynetworks.com)
As part of our expanded portfolio of new-generation instruments, the Orbitrap Exploris 240 mass spectrometer delivers the high performance and versatility needed to drive productivity across multiple applications. (selectscience.net)
Dr. Timothy Garrett, associate professor, department of pathology, immunology and laboratory medicine, co-director of the Southeast Center for Integrated Metabolomics, University of Florida, said, "Using the Orbitrap Exploris 240 mass spectrometer has been extremely user friendly, with one-click calibration and method templates for fast instrument setup. (selectscience.net)
Built on the guiding principles of operational simplicity and reliability, the Orbitrap Exploris 240 MS combines more than 20 years of know-how with high-resolution capability and exceptional and consistent mass accuracy, empowering all users to fast-track their science. (thermofisher.com)

Known proteolytic events (from the MEROPS peptidase database) were mapped to the dataset, allowing the identification of potential proteases and which substrates they cleave. (lu.se)

Peptide mapping, a bottom-up approach to protein characterization, enables the profiling of a protein's primary structure as well as its post-translational modifications (PTMs) such as oxidation, deamidation, and glycosylation. (sigmaaldrich.com)

Her expertise is in mass spectrometry of protein, peptides, and complex products, and she is the lead for FDA's multi-attribute method (MAM) research. (fda.gov)

Recombinant Pin1 Protein in Vitro Peptide and Phosphorylation Mapping. (bioskinrevive.com)
Thus, the principle of protein or peptide haptenation could be used in in vitro assays to predict the sensitization potential of a new chemical entity. (cdc.gov)

Taking collagen peptides by mouth seems to improve skin hydration and skin elasticity in older people. (medlineplus.gov)
This distribution comprises a small fraction of protein-specific hydration sites, where water rotation is strongly retarded, and a dominant fraction of generic hydration sites, where water rotation is as fast as in the hydration shells of small peptides. (lu.se)

KBI's state-of-the-art mass spectrometry core facility delivers unparalleled structural characterization services to our clients. (kbibiopharma.com)
Progress in several laboratories over the past few years including substantial partial peptide and nucleotide sequence determination has greatly enhanced our knowledge of the structural properties of this large molecule (heterodimer = 465,000 daltons). (embl.de)

Functional amyloids play a beneficial role in a variety of physiologic processes (eg, long-term memory formation, gradual release of stored peptide hormones). (medscape.com)

A small fraction of the peptide (usually 1 microliter or less) is pipetted onto a MALDI target and a chemical called a matrix is added to the peptide mix. (wikipedia.org)
In this work we identified these protein subunits with the modern proteomic tool of one-dimensional SDS-PAGE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The three separated pMMO subunits of 45, 27, and 23 kDa were observed on the SDS-PAGE and identified as the expression gene products of the pmoB, pmoC, and pmoA, respectively. (ncl.edu.tw)

The chemical reactions on these modifications change the peptide mapping pattern, and information from these reactions may contribute characterization and location of post-translational modified groups in the protein. (nih.gov)
Our expert team brings decades of experience in protein and peptide characterization to provide the right data with the right insight at all stages of development. (kbibiopharma.com)

A peptide-based enzyme-linked immunosorbent assay exists and signals the need for continued surveillance with (ELISA) can be used for retrospective serosurveillance of laboratory testing. (cdc.gov)
The new tion (RT-PCR) for detecting viral RNA is the most sensi- peptide ELISA consistently detected seroconversion by tive method for early identification of SARS. (cdc.gov)
The results from a retrospective serologic survey by the peptide ELISA of healthcare workers from facilities affected by nosocomial outbreaks are presented as a working example. (cdc.gov)

The advantage of this method is that only the masses of the peptides have to be known. (wikipedia.org)
In this work, we utilized a mass spectrometry-based peptide mapping approach to detect and quantitate the Asp isomerization in the CDRs of mAb-A. To routinely monitor the CDR isomerization of mAb-A, a focused peptide mapping method utilizing reversed phase chromatographic separation and UV detection has been developed and qualified. (pda.org)
In previous work, our laboratory reported on Endopep-MS, a mass spectrometry-based activity method for detecting and differentiating BoNT/A, /B, /E, and /F in clinical samples. (cdc.gov)
This method can detect all seven known BoNT serotypes and involves incubating BoNT with a peptide substrate that mimics each toxin's natural in vivo neuronal protein target. (cdc.gov)

Lysine-specific crosslinking mass spectrometry provided distance constraints to select a homology model of the DNAJB6 monomer, which was subsequently used in crosslink-assisted docking to generate a dimer model, revealing that a peptide-binding cleft lined with S/T-residues is formed at the monomer-monomer interface. (lu.se)

In this presentation, we will discuss the development of in situ/vivo approaches by cellular and subcellular mass spectrometry that enabled our lab to determine the proteomic and metabolomic profile of identified cells in live Xenopus laevis frog embryos developing to tadpoles and neurons in mouse brain tissues. (njacs.org)

One approach to achieving a non-redundant quantitation of phospho-motifs is to quantify each peptide but only assign a reference peptide, according to some quality criteria, as the quantification of the motif. (biorxiv.org)

Collagen peptides are very small pieces of protein from animal collagen. (medlineplus.gov)

The instrument's detector records the ions in proportion to their relative abundance and generates a mass spectrum of the molecule. (sigmaaldrich.com)
We found that the increased abundance of cleaved APOA1 peptides is likely a consequence of enzymatic degradation by metalloproteinases and chymase. (lu.se)

Anatomy17

Organisms13

Chemicals and Drugs114

Analytical, Diagnostic and Therapeutic Techniques and Equipment53

Phenomena and Processes57

Disciplines and Occupations3

Information Science6

Health Care4

Proteins23

Liquid chromatograp3

Proteomics5

Tandem Mass Spectro1

Enhanced mass spectrometry1

Tryptic3

Cleavage3

Capillary electrophoresis5

Antibody5

Trypsin3

Amino1

Sequences4

Intact4

Analysis16

Analytical11

Digestion1

Quantitative4

Amyloid2

Workflows1

Digests1

Workflow1

Monoclonal1

Antigens1

Synthetic3

Spectrometers2

High-resolution mass spectrometer1

Thermo Scientific9

HPLC1

Molecules6

Orbitrap5

Cleave1

PTMs1

Multi-attribute method1

Vitro2

Hydration2

Structural2

Hormones1

MALDI2

Characterization2

ELISA3

Method4

Generate1

Proteomic1

Quantitation1

Small1

Abundance2