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Complex MixturesMolecular WeightChromatography, GelMolecular Sequence DataProtein BindingAmino Acid SequenceElectrophoresis, Polyacrylamide GelMass SpectrometrySpectrometry, Mass, Electrospray IonizationTandem Mass SpectrometryProteomicsPolycyclic CompoundsAir PollutantsHydrocarbonsChromatography, LiquidChromatography, High Pressure LiquidSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationFuel OilsEnvironmental MonitoringSmogPetroleumPolycyclic Hydrocarbons, AromaticPolychlorinated BiphenylsGas Chromatography-Mass SpectrometryEnvironmental PollutantsWater Pollutants, ChemicalPeptidesProteomeBody Mass IndexCoal TarOdorsBenzo(a)pyreneMolecular StructureOrganic ChemicalsProteinsUnited States Environmental Protection AgencyVolatilizationAir Pollution, IndoorMutagenicity TestsDioxinsChromatography, GasToxicity TestsChemical FractionationDNA AdductsHazardous WasteIonsParticulate MatterChemistry Techniques, AnalyticalElectrophoresis, MicrochipCoalBenzene DerivativesChromatography, Ion ExchangeMagnetic Resonance SpectroscopyCarcinogensCarbohydrate SequenceSmokeChromatography, Thin LayerGasesBiological AssayPlant ExtractsCattleXenobioticsBenzopyrenesPeptide MappingAerosolsBenzofuransEnvironmental ExposureSensitivity and SpecificityOligosaccharidesSolventsReproducibility of ResultsMetabolomicsParticle SizeCytochrome P-450 CYP1A1SmellMutagensVehicle EmissionsIsotope LabelingBase SequenceOccupational ExposureTrypsinGlycopeptidesEpidemiological MonitoringAmino AcidsCell ExtractsTime FactorsPeptide FragmentsBiotransformationDustAlgorithmsKineticsElectrophoresis, Gel, Two-DimensionalIsomerismEstersPolysaccharidesTobacco Smoke PollutionDose-Response Relationship, DrugModels, BiologicalNanotechnologyTetrachlorodibenzodioxin
Resolve complex mixturesProteinsSpectrometerCharacterizationLipidElectrosprayPolycyclicQuantitationSpectraSpectrometricTandemSpeciesChromatography and massDigestionConstituentsSpectrometry methods2019PeptideMoleculesHRMSAnalysis of complexInvolvesAnalytical chemistrySamplesSuitableFractionsFatty acidDetectionTissuesIonizationTrypticIdentifyAdvantagesDataPrimarilyCompoundsMolecular structureIdentificationFoundConcentrationAluminumLiquidRapidLaboratoryContent
Resolve complex mixtures2
  • Here, we show that nonaqueous reversed-phase chromatography can be coupled to mass-spectrometry detection to resolve complex mixtures in minute (∼100 ng) samples of gold MPCs, of molecular masses up to ∼40 kDa, and with single-species sensitivity easily demonstrated for components on the level of sub-10 ng or picomole (1 pmol). (nau.edu)
  • We use gas chromatography (GC) to resolve complex mixtures of gases, and volatile and semi-volatile compounds, coupled with mass spectrometry (GC-MS) and other detectors such as flame ionisation (FID) and electron capture (ECD). (ncl.ac.uk)
Proteins8
  • We used consecutive orthogonal separation platforms to ensure sensitive detection: (a) ion-exchange of intact proteins, (b) SDS-PAGE separation of ion-exchange fractions and (c) HPLC separation of tryptic digests coupled to electrospray tandem mass spectrometry. (hindawi.com)
  • While modern proteomics experiments permit the analysis of hundreds to thousands of proteins in complex samples, the most powerful use of this data would combine information on protein activity with the identities of the active proteins. (hindawi.com)
  • Mass spectrometry-based proteomics has become a dominating approach for identification and quantitation of proteins from complex biological samples 1 . (springer.com)
  • For example, a number of research groups in CPTAC (Clinical Proteomic Tumor Analysis Consortium) 4 , 5 use isobaric labeling experiments, such as iTRAQ (isobaric Tags for Relative and Absolute Quantitation) or TMT (Tandem Mass Tag), to quantify thousands of proteins for cancer tissue samples from hundreds of patients. (springer.com)
  • The latter includes such capabilities as isotopic fine structure analysis for macromolecules in a wide mass range (and thus more confident elemental analysis of macromolecules) and higher sequence coverage in top-down analysis of proteins and protein complexes (including monoclonal antibodies and antibody-drug conjugates). (epfl.ch)
  • We extended the method to lean tissues and more hydrophilic chemicals by implementing a mass-balance model for partitioning between lipids, proteins, and water in tissues and by accelerating uptake kinetics with a custom-built stirrer that effectively decreased time to equilibrium to less than 8 days even for a homogenized liver tissue with an only 4% lipid content. (ufz.de)
  • The technique's high-throughput nature allows researchers to analyze complex mixtures of proteins and other biomolecules present in patient samples. (aurorabiomed.com)
  • Interpreting proteomics data remains challenging due to the large number of proteins that are quantified by modern mass spectrometry methods. (stanford.edu)
Spectrometer10
  • This work expands upon the separate LC and MS results previously achieved, by interfacing the capillary liquid chromatograph directly to the electrospray source of the mass spectrometer, in order to provide an extremely sensitive, quantitative, and rapid means to characterize MPCs and their derivatives far beyond that of earlier reports. (nau.edu)
  • Each of the mass spectrometer interfaces used in this study has particular advantages: the moving belt permits the acquisition of true electron impact- or chem. (canada.ca)
  • In particular, a 12 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer is used to characterize petroleum samples, such as crude oils, as part of the field that has become known as 'petroleomics. (warwick.ac.uk)
  • This peptide mixture can be used to test a Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight (MALDI-TOF) or Electrospray Ionization (ESI) mass spectrometer (TOF, Q-TOF or Ion Trap). (neb.com)
  • Eluting peptides were analyzed online by a Q-Exactive mass spectrometer (Thermo Scientific) with an electrospray ion source. (neb.com)
  • A mass spectrometer and method of mass spectrometry are disclosed wherein two separate samples are mass analysed and then the relative intensity, concentration or expression level of one or more components, molecules or analytes in a first sample is quantitated relative to the intensity, concentration or expression level of one or more components, molecules or analytes in a second sample. (gc.ca)
  • The laboratory also has LC-MS systems and a thermogravimetric analyser interfaced to a mass spectrometer (TGA-MS). (ncl.ac.uk)
  • The Netzsch Jupiter STA 449C is a thermogravimetric analyser interfaced to an Aeolos QMS 403 quadrupole mass spectrometer. (ncl.ac.uk)
  • Finally, most of the identities were unequivocally confirmed by accurate mass measurements on the TOF spectrometer. (unboundmedicine.com)
  • Anions elute in this separation in order of valency on the anion exchange column but are detected as singly-charged ions in the mass spectrometer. (thermofisher.com)
Characterization5
  • Straight-forward mass characterization of the complex mixtures could be achieved. (degruyter.com)
  • My research is focussed upon the characterization of complex mixtures using ultrahigh resolution mass spectrometry. (warwick.ac.uk)
  • Through the molecular characterization of complex mixtures, containing tens of thousands of components, profiles can be established for individual samples, serving as fingerprints. (warwick.ac.uk)
  • We conclude that this analytical platform is effective for the characterization of complex bioactive samples. (hindawi.com)
  • I am mainly working with i) instrumental characterization/profiling of complex multicomponent systems (body fluids and tissue) and development of data-analytical methods and algorithms for resolution, exploration, pattern recognition, discrimination and classification of such systems, and, ii) applications of such methods to solve multifaceted problems of medical and pharmaceutical origin. (uib.no)
Lipid5
  • One of the challenges of lipid identification using mass spectrometry is the large number of possible lipid species that can be present in a sample. (premierbiosoft.com)
  • This is particularly important in the analysis of complex lipid mixtures, where the abundance of different lipid species can vary significantly. (premierbiosoft.com)
  • The study of lipid biology is undergoing a remarkable, technology-driven transformation that most notably involves mass spectrometry (MS) and its ancillary techniques, such as liquid chromatography (LC) and ionization sources. (chromatographyonline.com)
  • As we move into a new era of lipid analysis, the potential to accurately and rapidly measure hundreds of individual molecular species provides the opportunity to use more complex lipid profiles for drug discovery and disease diagnostics. (chromatographyonline.com)
  • Equilibrium passive sampling employing polydimethylsiloxane (PDMS) as a sampling phase can be used for the extraction of complex mixtures of organic chemicals from lipid-rich biota. (ufz.de)
Electrospray1
  • We use a combination of MS techniques, namely liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) on a triple quadrupole, for the identification of the polyphenolic constituents of the soluble fraction of GADF. (unboundmedicine.com)
Polycyclic1
Quantitation1
  • Mass spectrometry-based proteomics using isobaric labeling for multiplex quantitation has become a popular approach for proteomic studies. (springer.com)
Spectra2
Spectrometric2
  • mass spectrometric studies, of the kinds outlined here, can be of great benefit in subdividing complex environmental or petroleum-derived mixts. (canada.ca)
  • mass spectrometric detn. (canada.ca)
Tandem2
Species1
Chromatography and mass1
  • Analytical instrumentation such as NMR, chromatography and mass spectroscopy combined with chemometrics represents key technologies for pattern recognition and biomarker discovery for early diagnosis, risk analysis, and, monitoring of disease development and therapeutic effects. (uib.no)
Digestion2
  • The end product of protein digestion is a complex mixture of primarily dipeptides and tripeptides, along with individual amino acids [ 14 ]. (springer.com)
  • A complex mixture of peptides produced by Trypsin digestion of Bovine Serum Albumin (BSA) that was reduced and alkylated with Iodoacetamide (CAM modified). (neb.com)
Constituents2
  • Opportunities and limitations for untargeted mass spectrometry metabolomics to identify biologically active constituents in complex natural product mixtures. (uib.no)
  • First, we separated the mixture into four fractions which were tested for phenolic constituents using the TOF system in the full scan mode. (unboundmedicine.com)
Spectrometry methods1
20191
  • 2019. Grouping of complex substances using analytical chemistry data: A framework for quantitative evaluation and visualization. (nih.gov)
Peptide1
Molecules6
  • This technique involves ionizing the sample molecules and then separating and detecting the resulting ions based on their mass-to-charge ratios. (premierbiosoft.com)
  • Triacylglycerols (TAGs) are complex lipids that consist of three fatty acid molecules esterified to a glycerol molecule. (premierbiosoft.com)
  • Phospholipids are complex lipids that contain a phosphate group, a glycerol molecule, and two fatty acid molecules. (premierbiosoft.com)
  • Due to their application in diverse fields, for molecules of very broad mass ranges, and by operators of very different expertise and knowledge, Orbitrap instruments are greatly simplified by the manufacturer (Thermo Fisher Scientific). (epfl.ch)
  • 2. A method as claimed in claim 1, further comprising providing a plurality of further samples each comprising a mixture of components, molecules or analytes. (gc.ca)
  • The dispersion amplitude requires only three (apo) or four (holo) long-lived and ordered water molecules (residence time 0.01-4 ms at 300 K). Comparison of MRD pro®les from the different complexes indicates that the displaced cavity water molecules are short-lived. (lu.se)
HRMS2
Analysis of complex3
  • Analysis of complex mixtures of intact monolayer-protected clusters (MPCs) by liquid chromatography mass spectrometry (LC-MS) could provide quantitative identification of the various components present. (nau.edu)
  • Master and PhD projects are provided within the areas of new algortihms and methods for profiling and data analysis of complex systems, and, application of such methods to solve medical and pharmaceutical problems. (uib.no)
  • Mass spectrometry coupled to liquid chromatography is a powerful tool for the analysis of complex plant derivatives such as GADF. (unboundmedicine.com)
Involves2
Analytical chemistry1
Samples3
  • Complex biological samples have also been successfully separated in multiple dimensions at the protein level. (hindawi.com)
  • Mass spectrometry (MS) is a powerful analytical technique that can be used to identify and characterize lipids in complex biological samples. (premierbiosoft.com)
  • The environmental mass spectrometry laboratory has over 30 years of experience in the analysis of soil and sediments, and gas samples. (ncl.ac.uk)
Suitable2
  • Thus, pathogens must have specific mecha- clearly complex, and, in many cases, the study of these nisms for mediating colonization, avoiding the host's interactions is limited by the lack of a suitable animal immune system, and acquiring necessary nutrients. (cdc.gov)
  • We are proud to introduce two ready-to-use certified reference material mixtures suitable for use as calibrants for the above mentioned new IFRA method. (sigmaaldrich.com)
Fractions2
  • In the qNMR measurements, a value for the mass fractions (g/g) was obtained, and direct traceability to the SI unit was achieved through the use of acknowledged NIST primary reference materials. (sigmaaldrich.com)
  • The elucidation of such lignin fractions properties is crucial in the direction of understanding its complex molecular structure, boosting its homogeneity, and fostering technologies for advanced lignin-based materials. (lu.se)
Fatty acid1
  • the sucrose fatty acid esters are mixture of two types of nonionic surfactants: sucrose palmitates and sucrose stearates. (degruyter.com)
Detection1
  • This new analytical method has been developed by the IFRA Analytical Working Group to identify and to quantify the 57 fragrance substances and their relevant isomers at a concentration higher than 0.002% (20 mg/kg) in ready to inject fragrance materials and oils using Gas Chromatography with FID (GC-FID) or with Mass Spectrometry (GC-MS) detection. (sigmaaldrich.com)
Tissues1
Ionization3
  • Among the various diagnostic techniques available, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) has revolutionized clinical diagnostics. (aurorabiomed.com)
  • This technique enables rapid and accurate identification of microorganisms by creating a unique "fingerprint" for microbial cells or components through ionization and mass analysis. (aurorabiomed.com)
  • Large-gel two-dimensional electrophoresis-matrix assisted laser desorption/ionization-time of flight-mass spectrometry: an analytical challenge for studying complex protein mixtures. (mpg.de)
Tryptic1
  • This standard is a complex mixture of peptides produced by the tryptic digest of reduced and alkylated Bovine Serum Albumin (BSA). (neb.com)
Identify2
  • One of the main advantages of mass spectrometry is that it can identify lipids without the need for separation or purification steps. (premierbiosoft.com)
  • Histone H3 lysine 27 acetylation (H3K27ac), histone H3 lysine 4 monomethylation (H3K4me1), bromodomain containing 4 (BRD4) and mediator complex subunit 1 (MED1) were utilized by the ROSE algorithm to identify SEs. (nature.com)
Advantages1
  • This two-part column explains how lipids analysis has evolved and the variety of techniques and approaches currently used in lipidomics, including their advantages and disadvantages, such as sample preparation, separations and, of course, mass spectrometry. (chromatographyonline.com)
Data2
Primarily1
  • D Street West Broadway South real estate is primarily made up of small (studio to two bedroom) to medium sized (three or four bedroom) small apartment buildings and apartment complexes/high-rise apartments. (neighborhoodscout.com)
Compounds1
  • A range of column types are available for gas, polar and non-polar compounds to provide optimised separation of complex matrices backed up by the knowledge and experience of the technical staff. (ncl.ac.uk)
Molecular structure1
  • Mass spectrometry can provide information on the molecular structure of lipids, including the number and position of double bonds, the length of the acyl chains, and the presence of functional groups such as phosphates and sugars. (premierbiosoft.com)
Identification1
  • This advanced technology can rapidly analyze biomolecules based on their mass-to-charge ratios, leading to diverse applications that span microbial identification, disease diagnosis, and personalized medicine. (aurorabiomed.com)
Found4
  • It was found that the composition of the collagen hydrolysates prior to and after ingestion was highly complex and dynamic, which made it challenging to predefine a strategy for a targeted analysis. (springer.com)
  • Table 1 contains a collection of the largest m/z ions found and the associated mass accuracy information. (thermofisher.com)
  • It is important to correlate mass accuracy of a found mass to the proposed chemical formula in order determine if the proposed chemical formula is reasonable. (thermofisher.com)
  • Ce 3+ was found to form highly charged complexes with a mean coordination number between 7 and 8, with the shell containing mostly chloride, followed by water. (lu.se)
Concentration1
  • The mean mass concentration of particles ranged from 190 to 847 microg/m3. (cdc.gov)
Aluminum1
  • Modern premium cover glass is usually based on a mixture containing lithium and aluminum. (schott.com)
Liquid3
  • The analysis of protein hydrolysates using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) can be complex. (springer.com)
  • This honey-like mass floats in a liquid tin bath, is drawn to the desired thickness, and then cooled in a controlled manner. (schott.com)
  • The environmental mass spectrometry laboratory can perform both routine and bespoke GC and GC-MS analysis with integrated liquid and pyrolysis autosamplers, and gas sampling valves. (ncl.ac.uk)
Rapid1
  • We are currently accepting coomassie and silver stained gel bands and spots for mass spectrometry sequencing, and coomassie stained gel bands and spots for rapid gel band ID or protein modification analysis. (mskcc.org)
Laboratory1
  • After all, glass that looks good on paper and in the laboratory also has to be viable for mass production. (schott.com)
Content2
  • Doing so reduces the solid content of the plastic to about 40 percent of its original mass, and that material can be landfilled. (resource-recycling.com)
  • Murine studies have cyanates and other chemicals, with highly variable vola- demonstrated that dose, route and timing of isocyanate tility, mass, solubility and isocyanate content [5] . (cdc.gov)