• Cancer stem cells (CSCs) play an important role during metastasis, but the dynamic behavior and induction mechanisms of CSCs are not well understood. (nature.com)
  • Here, we employ high-resolution intravital microscopy using a CSC biosensor to directly observe CSCs in live mice with mammary tumors. (nature.com)
  • CSCs display the slow-migratory, invadopod-rich phenotype that is the hallmark of disseminating tumor cells. (nature.com)
  • CSCs are enriched near macrophages, particularly near macrophage-containing intravasation sites called Tumor Microenvironment of Metastasis (TMEM) doorways. (nature.com)
  • At the apex of the hierarchy are the cancer stem cells (CSCs). (nature.com)
  • CSCs have an enhanced ability to self-renew, which makes them uniquely capable of initiating and sustaining primary and metastatic tumor growth 3 . (nature.com)
  • SOX9 depletion also suppressed the formation of cancer stem-like cells (CSCs), as determined by tumor sphere formation and aldehyde dehydrogenase (ALDH) activity (Aldefluor) assays. (cdc.gov)
  • Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. (wikipedia.org)
  • CSCs are therefore tumorigenic (tumor-forming), perhaps in contrast to other non-tumorigenic cancer cells. (wikipedia.org)
  • CSCs may generate tumors through the stem cell processes of self-renewal and differentiation into multiple cell types. (wikipedia.org)
  • As CSCs form a small proportion of the tumor, this may not necessarily select for drugs that act specifically on the stem cells. (wikipedia.org)
  • Hence, at least for certain neoplastic tissues, dedicated stem cell compartments maintain and enlarge the size of the transformed compartment The cancer stem cell model, also known as the Hierarchical Model proposes that tumors are hierarchically organized (CSCs lying at the apex (Fig. 3). (wikipedia.org)
  • Essentially this theory proposes that all cells have the ability to be tumorigenic making all tumor cells equipotent with the ability to self-renew or differentiate, leading to tumor heterogeneity while others can differentiate into non-CSCs The cell's potential can be influenced by unpredicted genetic or epigenetic factors, resulting in phenotypically diverse cells in both the tumorigenic and non-tumorigenic cells that compose the tumor. (wikipedia.org)
  • This observation has led to the 'cancer stem cell' (CSCs) hypothesis, stating that a subpopulation of cancer cells survive therapy and lead to tumor relapse. (mdpi.com)
  • Cancer stem cells (CSCs) are thought to the underlying reason for cancer initiation, metastasis, and relapse. (springer.com)
  • These studies suggest that there is a bi-directional crosstalk between macrophages and CSCs that promotes stemness renewal and tumor associated macrophage polarization. (springer.com)
  • Cancer stem cells (CSCs) represent a small subset of the tumor population and reside at the apex of the hierarchy [ 9 ]. (springer.com)
  • Traditionally, CSCs are identified through a combination of surface markers, however, there is a lack of consensus among the scientific community, especially as these combinations appear to be specific to the corresponding tissues and tumors. (springer.com)
  • Apart from CSCs, there are a myriad of factors and interactions between various cell types and the tumor microenvironment (TME) that ultimately affect CCA progression. (springer.com)
  • A subset of cancer cells undergoing EMT acquire characteristics of stem cells and are designated as cancer stem cells (CSCs) [ 8 ]. (techscience.com)
  • this condition may be due to the existence of the cancer stem cells (CSCs) that are resistant to the hormone therapy. (jcancer.org)
  • Cancer stem-like cells (CSCs) are associated with cancer progression, metastasis, and recurrence, and may also represent a subset of circulating tumor cells (CTCs). (biorxiv.org)
  • Results CSCs of A549 cell were successfully enriched by and the percentage of GD2 + cells was 0.57% in adherent cell, but the percentage of GD2 + cells was able to increase to 20.4% after spheroid culturing. (magtechjournal.com)
  • Nonetheless the forms of cells CSCs can grow to be restricted in a grim means. (apklas.com)
  • Cancer stem cells generally known as CSCs are a particular kind of stem cell present in some types of most cancers together with malignant mesothelioma. (apklas.com)
  • The derived MPeMSCs present important expression of MSLN a outstanding marker for mesothelioma indicating that CSCs originated from the parental mesothelioma cells. (apklas.com)
  • Like all stem cells CSCs have the potential to grow to be numerous different cell sorts. (apklas.com)
  • Putative CSCs type extremely metastatic tumors in NOD SCID mouse. (apklas.com)
  • CSCs can grow to be any kind of tumor-specific cell within the human physique so simply as wholesome stem cells from bone marrow can grow to be wholesome pores and skin or muscle tissue CSCs can grow to be any cell. (apklas.com)
  • CSCs are extra proof against chemotherapy than the differen-tiated cells of the tumor 12 by means of their quiescence capability to restore DNA and adaptation to hypoxic environments 13. (apklas.com)
  • Stable tumors include a uncommon inhabitants of most cancers stem cells CSCs which might be answerable for relapse and metastasis. (apklas.com)
  • The therapy (CLBS20, also known as NBS20) uses purified autologous CSCs, derived from a patient's own tumor and grown as a tumor cell line, as the antigen source of tumor-associated antigens to induce or enhance an anti-tumoral immune response. (bmj.com)
  • Efforts to link CD44 to CSCs and tumor progression should consider the expression of various CD44 isoforms. (lu.se)
  • This review summarizes the current understanding of the regulatory mechanisms of epithelial-mesenchymal transition (EMT) in cancer stem cells (CSC) initiation, with a special focus on the role of microRNAs in EMT control, and a discussion the implications of targeting CSCs for cancer therapeutics. (stemcellsciencenews.com)
  • Emerging evidence suggests that cancer stem cells (CSCs), a subpopulation of tumor cells, have the properties of self-renewal, heterogeneous progeny, drug-resistance, and carcinogenesis in vitro and in vivo ( 4 , 5 ). (spandidos-publications.com)
  • Therefore, a therapeutic strategy targeting CSCs may help cure malignant tumors, including SCLC ( 7 ). (spandidos-publications.com)
  • It is known that GBM involves cancer stem-like cells (CSCs) and tumor cells that aggressively invade normal brain tissues, and both cell types may cause resistance to radiotherapy (RT) and are thus responsible for therapeutic failure. (tmu.edu.tw)
  • CSCs are capable to self-renew, differentiate and initiate tumor formation. (molcells.org)
  • In this review, we will discuss the recent findings on hepatic CSCs, with special emphasis on their putative origins, relationship with hepatitis viruses, regulatory signaling networks, tumor microenvironment, and how these factors control the stemness of hepatic CSCs. (molcells.org)
  • This review summarizes recent discoveries relevant to liver CSCs, with emphasis on discussing their putative cellular origins, the roles of hepatitis viruses, tumor microenvironment and signaling network in controlling the stemness of CSCs. (molcells.org)
  • Glioblastoma multiforme (GBM) is the most common brain tumour, characterized by a central and partially necrotic (i.e., hypoxic) core enriched in cancer stem cells (CSCs). (omicsdi.org)
  • CSCs) GBM cells were resistant to Temozolomide (TMZ) in vitro, owing to a particularly high expression of O6-methylguanine-DNA-methyltransferase (MGMT), the most important factor associated to therapy resistance in GBM. (omicsdi.org)
  • Here, we demonstrate that scL crosses the BBB and efficiently targets GBM, as well as cancer stem cells (CSCs), which have been implicated in recurrence and treatment resistance in many human cancers. (omicsdi.org)
  • scL-p53 also sensitized both CSCs and bulk tumor cells to TMZ, increasing apoptosis. (omicsdi.org)
  • One of the possible reasons for the failure of the present therapeutic regimens is the existence of a relatively rare cell population known as cancer stem cells (CSCs) that are resistant to or not targeted by present therapeutic modalities. (docksci.com)
  • There are considerable data indicating that CSCs could be responsible for complex tumor biology, including initiation, growth, and regeneration. (docksci.com)
  • In 1997, Bonnet & Dick (1997) published their findings concerning the existence of CSCs, by isolating a subpopulation of leukemic cells. (docksci.com)
  • 2009). Nanomaterials, such as nanotubes, nanoparticles, and polymers, are promising materials that need to be explored with increased attention owing to their potential as components of an alternative treatment approach for tumors and CSCs (Orza et al. (docksci.com)
  • BACKGROUND: Cancer stem cells (CSCs) with a self-renewal ability in tumor cells population, execute a pivotal function in tumorigenesis, retrogression, and metastasis of malignant cancers such as anaplastic thyroid carcinoma (ATC).MATERIALS AND METHODS: In this study, we isolated CSCs subpopulation with CD133 surface marker from three ATC cell lines by magnetic cell sorting assay. (moleculardepot.com)
  • Pancreatic cancer is enriched with cancer stem cells (CSCs), which contribute to its high metastatic tendency and resistance to treatments. (ku.edu)
  • The formation of CSCs is highly associated with cancer cell epithelial-mesenchymal transition (EMT), which contributes to the plasticity of cancer cells and comprises an initial step for metastasis. (ku.edu)
  • Various efforts have been made to develop anti-tumor agents through modulation of important signaling pathways involved in EMT and CSCs. (ku.edu)
  • The data demonstrated that inhibition of HuR destabilized SNAI1 mRNA, and subsequently reduced Snail protein levels, resulting in the EMT and CSCs inhibition in pancreatic cancer cells. (ku.edu)
  • Cancer stem cells (CSCs) are the subpopulation of cells within a tumor proposed to be responsible for tumor initiation, relapses, and resistance to chemotherapeutic drugs. (hilarispublisher.com)
  • Here we optimized sphere culture conditions to isolate and enrich CSCs from colorectal cancer cell line IMCE-Ras. (hilarispublisher.com)
  • Xenograft transplantation assays further demonstrated that IMCE-Ras spheroid cells are highly enriched in CSCs. (hilarispublisher.com)
  • To develop CSC-targeted therapy, we found that the relative percentage of CSC in IMCE-Ras cells was significantly decreased after a short duration exposure to DNA Methylation inhibitor 5-aza-2'-deoxycytidine (5-Aza-dC), indicating that DNA Methylation may be critical for self-renewal and maintenance of CSCs. (hilarispublisher.com)
  • OS cancer stem cells (CSCs) also are considered a key factor of tumorigenesis, drug resistance and tumor recurrence. (biomedcentral.com)
  • CD271+ OS CSCs and CD271- OS cells were isolated by magnetic activated cell sorting. (biomedcentral.com)
  • Our data indicated that CD271+ OS CSCs had a similar basic autophagy level with CD271- OS cells. (biomedcentral.com)
  • The results above demonstrated that autophagy contributes to the stem-like features of CD271+ OS CSCs. (biomedcentral.com)
  • Cancer stem cells (CSCs) are seen as a subpopulation of self-renewing tumor cells, which can differentiate into the daughter tumor cells, have the lower sensitivity to chemotherapy and radiotherapy, and exhibit tumor re-initiating property. (biomedcentral.com)
  • CD271, an MSC antigen, was likewise identified as an effective OS-CSCs marker. (biomedcentral.com)
  • We found that SOX9 is upregulated in SWCNT-exposed cells, which is consistent with their abilities to induce tumor formation and metastasis in vivo. (cdc.gov)
  • Furthermore, SOX9 knockdown suppressed tumor metastasis and the expression of the stem cell marker ALDH1A1. (cdc.gov)
  • Such cells are hypothesized to persist in tumors as a distinct population and cause relapse and metastasis by giving rise to new tumors. (wikipedia.org)
  • Epithelial-mesenchymal transition (EMT) is a dynamic process involved in cancer metastasis wherein cancer cells with epithelial characteristics acquire mesenchymal characteristics such as increased motility, invasion, and survival [ 6 ]. (techscience.com)
  • Combined, these data indicate that CD117 expression potentially promotes tumor initiation and metastasis. (biorxiv.org)
  • Overexpression of this gene is associated with tumor cell proliferation and metastasis. (cancerindex.org)
  • DRAM2 was preferentially upregulated in NSCLC tissues and higher expression of DRAM2 in NSCLC correlated with tumor node metastases stage and lymph node metastasis. (cancerindex.org)
  • Additionally, DRAM2 overexpression promoted cell metastasis and proliferation in vitro, while knockdown of DRAM2 expression yielded opposite result. (cancerindex.org)
  • These results indicate that CTCDOs recapitulate several features of colorectal CTCs and may be used to investigate the features of metastatic CRC cells, to identify new prognostic biomarkers and to devise new potential strategies for metastasis prevention. (biomedcentral.com)
  • Circulating tumor cells (CTCs) contain the physical elements responsible for tumor dissemination and metastasis. (biomedcentral.com)
  • Caladrius Biosciences is developing an innovative approach to cancer treatment targeting the cells responsible for tumor growth and metastasis, known as cancer stem cells (CSC) or tumor-initiating cells (TIC). (bmj.com)
  • CD24 is a hepatic cancer stem cell biomarker that is closely associated with apoptosis, metastasis and recurrence of hepatocellular carcinoma. (stemcellsciencenews.com)
  • Among others are the breast cancer stem cells (BCSCs), which are believed to have a contribution to tumorigenesis, metastasis, and resistance against chemotherapy. (hindawi.com)
  • In this study, we sought to investigate the mechanism underlying the inhibitory effect of PPI on the invasion and metastasis of SR-HCC cells. (bvsalud.org)
  • Interference with and overproduction of GRP78 in vitro impacted the proliferation, migration, invasion, and metastasis of HCC cells. (bvsalud.org)
  • The results indicated that PPI administration decreased the levels of proteins associated with metastasis and markers of drug resistance in tumor tissues, impeding tumor growth and spread. (bvsalud.org)
  • In an orthotopic pancreatic cancer mouse model, KH-3 significantly inhibited tumor growth and metastasis. (ku.edu)
  • Due to the insidious identity of HCC, the majority of the patients are diagnosed at advanced stages, where tumor spreading, or distant metastasis unfortunately have already occurred. (stanford.edu)
  • Additionally, characterization of the tumor microenvironment such as interactions with immune cells remain largely unknown. (springer.com)
  • Specifically, the concepts of tumour heterogeneity, oncogene addiction, non-oncogene addiction, tumour initiating cells, tumour microenvironment, non-coding sequences and DNA damage response will be reviewed. (bmj.com)
  • Adipocytes represent a major cell type in the mammary tumor microenvironment and are important for tumor growth. (jci.org)
  • Adipocyte-derived factors involved in tumor progression include proteins such as adiponectin, leptin, TNF-α, monocyte chemotactic protein-1 (MCP-1), IL-6, and ECM components that control tumor cell behavior within the tumor microenvironment. (jci.org)
  • The aim of this thesis was to evaluate how microenvironmental cues affect the interactions between the tumor microenvironment and glioma stem-like cells in glioblastoma. (lu.se)
  • It is the outcome of many factors, including the cross-talk between tumor cells within the tumor microenvironment and the acquisition and accumulation of genetic and epigenetic alterations in tumor cells. (molcells.org)
  • HCC heterogeneity is believed to be the outcome of combinatorial effects, including the acquisition of genetic and epigenetic alterations in tumor cells, the cross-talk of cells within the tumor microenvironment, as well as the existence of a cancer stem cell (CSC) subpopulation that has the ability to self-renew and differentiate. (molcells.org)
  • The previous work has shown that Tanshinone IIA could ameliorate inflammation microenvironment in colorectal cancer via repressing miRNA-155 expression ( 8 ) and exert an anti-angiogenic effect in vascular endothelial cells via inhibiting the VEGF/VEGFR2 pathway [ 9 ]. (biomedcentral.com)
  • Our team has developed a new biopsy technology that can profile multiple tumor microenvironment biomarkers simultaneously, revealing cellular spatial organization and interactions that will help advance personalized disease diagnosis and treatment. (aacu.org)
  • The recently described role of the Wnt/β-catenin pathway in the regulation of immune cell infiltration of microenvironment tumor resumes interest because of its potential impact to rection of immune therapy. (chemdiv.com)
  • Macrophages are emerging as major cellular factors in the tumor ecosystem that can influence the stem phenotype and cancer progression. (nature.com)
  • This model suggests that only certain subpopulations of cancer stem cells have the ability to drive the progression of cancer, meaning that there are specific (intrinsic) characteristics that can be identified and then targeted to destroy a tumor long-term without the need to battle the whole tumor. (wikipedia.org)
  • EIF5B (Eukaryotic Translation Initiation Factor 5B) is a conserved eukaryotic translation factor that mediates association of the 40S and 60S ribosomal subunits during eukaryotic translation initiation, and modulates the cell cycle progression by regulating the translation of upstream open reading frames (uORF)-containing mRNAs such as p27 and p21 [ 3 ]. (techscience.com)
  • These data suggest that EIF5B functions as an oncogene that promotes cancer cell growth, survival, and progression. (techscience.com)
  • A workshop was convened by the AACR to discuss the rapidly emerging cancer stem cell model for tumor development and progression. (aacrjournals.org)
  • Further, in cell lines, CD117 activation by SCF promotes faster proliferation and invasiveness, while blocking CD117 activation with tyrosine kinase inhibitors (TKIs) decreased progression in a context-dependent manner. (biorxiv.org)
  • In the presence of a genetic lesion, downregulation of SETD2 contributed to both initiation and progression during leukemia development by promoting the self-renewal potential of leukemia stem cells. (stemcellsciencenews.com)
  • In cancer progression, FOXO3a inhibition stimulates cell transformation and angiogenesis ( 9 ). (spandidos-publications.com)
  • We investigated the presence of aluminium (Al) in human colon cancer samples and its potential association with biological processes involved in cancer progression, such as epithelial to mesenchymal transition (EMT) and cell death. (bvsalud.org)
  • Despite these advances, we currently lack simple, highly sensitive, yet inexpensive methods to follow the progression of disease with associated genomic markers. (selectbiosciences.com)
  • These compounds contribute to the suppression of tumor progression and aggressiveness in various degrees. (ku.edu)
  • Combined with miRNAs-based transcriptome sequencing assay, it was found that Tanshinone IIA downregulated miR-125b level and upregulated its target gene STARD13 (StAR-related lipid transfer protein 13) level, thus inactivating the miR-125b/STARD13 axis, which had been previously confirmed to promote breast cancer progression. (biomedcentral.com)
  • SOX9 regulates cancer stem-like properties and metastatic potential exposed cells. (cdc.gov)
  • Moreover, ETP expression was associated with aggressive mammary tumor growth and high metastatic growth. (jci.org)
  • Circulating tumor cells (CTCs) are responsible for the metastatic dissemination of colorectal cancer (CRC) to the liver, lungs and lymph nodes. (biomedcentral.com)
  • CTCs analysis through liquid biopsies provides not only a full repertoire of tumor biological material (including proteins, lipids, sugars and nucleic acids) but also a sample of living tumor cells endowed with metastatic ability [ 3 ]. (biomedcentral.com)
  • The CD44 cell adhesion molecule is aberrantly expressed in many breast tumors and has been implicated in the metastatic process as well as in the putative cancer stem cell (CSC) compartment. (lu.se)
  • Scientists demonstrated that murine tumor propagating cells with the markers Sca1 and CD24 are enriched for metastatic potential in orthotopic transplantation assays. (stemcellsciencenews.com)
  • The regulation of the stem cell-like properties by several important signaling pathways have been found to endow the tumor cells with an increased level of tumorigenicity, chemoresistance, and metastatic ability. (molcells.org)
  • We have developed a systemic nanodelivery platform (scL) for tumor-specific targeting (primary and metastatic), which is currently in multiple clinical trials. (omicsdi.org)
  • Cancer stem cells are responsible for tumor chemoresistance and recurrence in adjuvant and metastatic settings. (isciii.es)
  • Dr. Lim has more than 18 years of experience in cell sorting, cell cycle analysis, apoptosis and immunophenotyping. (sc.edu)
  • Casticin, a natural polymethoxyflavone isolated from A. annua, V. trifolia, and V. agnus‑castus induces apoptosis in cancer cells by activating FoxO3a. (spandidos-publications.com)
  • Conversely, FOXO3a overexpression suppresses cancer cell growth, induces apoptosis, and reduces tumor size by regulating downstream effectors ( 10 ). (spandidos-publications.com)
  • Rapamycin induced the apoptosis of MG63 cells and this apoptosis was enhanced by Spautin-1. (exposed-skin-care.net)
  • It was considered that Spautin-1 suppressed the protective mechanism induced by rapamycin in tumor cells and induced apoptosis. (exposed-skin-care.net)
  • Result: MSI1 enhances tumor invasion via VCAM1 and modulates GBM radioresistance via the hyperactivation of the DDR through increasing homologous recombination repair and evading apoptosis. (tmu.edu.tw)
  • Here we show that BMP2 induced strong differentiation of GBM stem-like cells and subsequent addition of TMZ caused dramatic increase of apoptosis. (omicsdi.org)
  • Lots of the differentially governed genes were involved with inflammation and immune system response, STF-62247 cell adhesion, aswell as apoptosis (Fig. S1A). (pkc-inhibitor.com)
  • Abnormal proliferation, clonal expansion, aberrant differentiation, and diminished apoptosis (programmed cell death) lead to replacement of normal blood elements with malignant cells. (msdmanuals.com)
  • Both EMT and CSC phenotypic changes are associated with tumor recurrence. (techscience.com)
  • Therefore, EMT-targeted therapy is necessary to effectively overcome tumor drug resistance and recurrence. (techscience.com)
  • Scientists showed that a deep surgical margin was resected very close to the tumor site in many neoadjuvant chemotherapy (NAC)-treated oral squamous cell carcinoma patients, suggesting that NAC may lead to local recurrence and poor outcomes. (stemcellsciencenews.com)
  • This tumor subtype tended to show a worse clinical course, with earlier and more frequent recurrence and worse 5-year survival, compared with non-TNBC [ 4 ]. (hindawi.com)
  • Recurrence and chemoresistance in pancreatic cancer are largely related to cancer stem cells. (isciii.es)
  • Latest studies reveal cells heterogeneity in mind tumors, and there is certainly accumulating proof that tumor initiation, recurrence, and the reduced rate of success are likely based upon a part of glioblastoma stem cells (GSCs) that are chemo- and radio-resistant [6]C[11]. (irjs.info)
  • The results in vivo indicated that tumors formed by GD2 + A549 cells grew faster ( P <0.001) and were more malignant ( P <0.01) as compared to GD2 - A549 cells. (magtechjournal.com)
  • BACKGROUND: Glycolytic metabolic reprogramming is a phenomenon in which cells undergo altered metabolic patterns during malignant transformation, mainly involving various aspects of glycolysis, electron transport chain, oxidative phosphorylation, and pentose phosphate pathway. (bvsalud.org)
  • AB - Malignant mesothelioma MM is an aggressive and therapy-resistant neoplasm arising from the pleural mesothelial cells and normally related to long-term asbestos publicity. (apklas.com)
  • Inter- CD26 estingly whereas Meso-1 cells expressed each CD24 and CD26 the presence of every of those two markers Cancer stem cells Malignant mesothelioma was correlated with completely different CSC property. (apklas.com)
  • Human glioblastoma (GBM) is the most common primary malignant brain tumor. (nature.com)
  • Investigators found that spontaneously-formed tumorigenic hybrids between bone marrow-derived mesenchymal stem cells and three different non-small cell lung cancer cell lines contributed to highly malignant subpopulations with both epithelial-to-mesenchymal transition (EMT) and stem cell-like properties. (stemcellsciencenews.com)
  • Hepatocellular carcinoma (HCC), a highly malignant disease and the third leading cause of all cancer mortalities worldwide, often responses poorly to current treatments and results in dismal outcomes due to frequent chemoresistance and tumor relapse. (molcells.org)
  • Malignant major human brain tumor morbidity in america is just about 22,000 situations each year, and 80% are GBMs [2]C[4]. (irjs.info)
  • Osteosarcoma (OS) is one of the most common primary malignant bone tumors and mainly affects children, adolescents, and young adults (between the ages of 10 to 25) [ 1 ]. (biomedcentral.com)
  • Malignant plasma cells in meningeal MM out of 2000 patients with bone marrow produce an immunoglobulin, MM, was reported was by Schluterman et al. (who.int)
  • Also, the term "primary cutaneous CD4 + small/medium T-cell lymphoma" was changed to "primary cutaneous CD4 + small/medium T-cell lymphoproliferative disorder" because of its indolent clinical behavior and uncertain malignant potential. (medscape.com)
  • In acute myeloid leukemia (AML), malignant transformation and uncontrolled proliferation of an abnormally differentiated, long-lived myeloid progenitor cell results in high circulating numbers of immature blood cells and replacement of normal marrow by malignant cells. (msdmanuals.com)
  • Загальні відомості про лейкоз Leukemia is a malignant condition involving the excess production of immature or abnormal leukocytes, which eventually suppresses the production of normal blood cells and results in symptoms. (msdmanuals.com)
  • Malignant transformation usually occurs at the pluripotent stem cell level, although it sometimes involves a committed stem cell with more limited capacity for self-renewal. (msdmanuals.com)
  • Upon its transition to the precancerous Barrett's esophagus epithelium, however, a small dedicated stem cell compartment appears that supports proliferation of the epithelium while concomitantly evidence for a stochastically dividing compartment contributing to the maintenance of the tissue disappears. (wikipedia.org)
  • Down-regulation of EIF5B significantly decreased proliferation and invasiveness of the HCC cells. (techscience.com)
  • We demonstrate that CD117+ cells display increased proliferation and migration. (biorxiv.org)
  • In comparison with GD2 - A549 cells, GD2 + A549 cells showed significantly enhanced cell proliferation ( P <0.05),invasion ( P <0.05) and migration ability ( P <0.01) in vitro . (magtechjournal.com)
  • Transwell migration, cell cycle analysis, MTT and colony formation assays were performed to determine the effect of DRAM2 overexpression and knockdown on NSCLC-cell migration and proliferation. (cancerindex.org)
  • We found that rapamycin inhibited cell proliferation and decreased the phosphorylation of mTOR pathway components in MG63 cells. (exposed-skin-care.net)
  • Statistical evaluation Statistical analyses for BMS-536924 the cell proliferation assay had been performed using GraphPad Prism 5 software program (GraphPad NORTH PARK CA USA) with one- or two-way ANOVA accompanied by post hoc evaluation. (exposed-skin-care.net)
  • The cell proliferation level was detected with BrdU staining assay. (biomedcentral.com)
  • Autophagy deficiency had no observable effects on the levels of cell proliferation and death both in CD271+ and CD271- OS cells under normal condition. (biomedcentral.com)
  • In keeping with the accelerated tumor development a elevated proliferation STF-62247 price could possibly be discovered in neglected considerably, non-tumorous Dkk2? (pkc-inhibitor.com)
  • The gene product of CYLD down-regulates the NF-κB signaling pathway, and when this protein is nonfunctional, increased activity of the NF-κB pathway leads to uncontrolled cell proliferation, resulting in the development of adnexal tumors. (medscape.com)
  • In the present study, we investigated the expression profile and prognostic potential of EIF5B in HCC tissues and cell lines. (techscience.com)
  • In vivo experiments were verified by establishing a subcutaneous transplantation tumor model in nude mice, observing the size and volume of tumors in vivo in nude mice, and obtaining fresh tissues for subsequent experiments. (bvsalud.org)
  • Among these, Raman spectroscopy has attracted increasing attention as a highly sensitive molecular imaging technique for studying complex biological systems, including cells, tissues, and various biological materials [ 4 ]. (medsci.org)
  • This technique allows non-destructive, label-free spectral imaging and analysis of cells, tissues, and nanoparticles [ 5 - 7 ]. (medsci.org)
  • Lately, the breakthrough of miRNAs as motorists of tumor development and success has resulted in the evaluation from the miRNA appearance pattern in various types of glioblastomas when compared with normal brain tissues [25]C[28]. (irjs.info)
  • Termed the Multi Omic Single-scan Assay with Integrated Combinatorial Analysis (MOSAICA), the fluorescence imaging-based technology can spatially profile a large number of mRNA and protein markers in cells and tissues, including clinical tumor tissues. (aacu.org)
  • In vitro and in vivo testing of purified BTIC subsets were performed to verify multipotency, self-renewal, and capacity to generate glioblastoma tumors in murine cranial xenotransplantation models. (cns.org)
  • Both BTIC subtypes were validated in vitro and in vivo as demonstrating multi-potency, self-renewal, and capacity to generate glioblastoma tumors in murine xenotransplants. (cns.org)
  • The meeting participants were charged with evaluating data suggesting that cancers develop from a small subset of cells with self-renewal properties analogous to organ stem cells. (aacrjournals.org)
  • Indeed, one critical question contemplated at the Workshop was whether tumors derive from organ stem cells that retain self-renewal properties but acquire epigenetic and genetic changes required for tumorigenicity or whether tumor stem cells are proliferative progenitors that acquire self-renewal capacity. (aacrjournals.org)
  • Cancer stem cells are a population of slow-cycling cells within the tumour bulk, with self-renewal capacity that attracts interest as a therapeutic target. (lu.se)
  • BCSC is a subset of tumor cells that has stem cell-like characteristics, such as a high capacity for self-renewal and tumor initiation, which implies that BCSC may cause aggressiveness of TNBC. (hindawi.com)
  • Breast cancer stem cell is a subset of tumor cells that has a characteristic of stem cells, having a high capacity of self-renewal and tumor initiations, which imply that BCSC may cause aggressiveness of TNBC [ 5 ]. (hindawi.com)
  • A second trainee examined the role of Sae2 in DNA replication, a critical step in growth and self-renewal of pluripotent stem cells. (ca.gov)
  • In different tumor subtypes, cells within the tumor population exhibit functional heterogeneity and tumors are formed from cells with various proliferative and differentiation capacities. (wikipedia.org)
  • This functional heterogeneity among cancer cells has led to the creation of multiple propagation models to account for heterogeneity and differences in tumor-regenerative capacity: the cancer stem cell (CSC) and stochastic model. (wikipedia.org)
  • In this study, single-cell microfluidic genetic profiling of primary human glioblastoma was performed to characterize intra-tumoral BTIC heterogeneity, identify unique surface markers of BTIC subsets, and associate BTIC subsets with clinically-relevant bulk tissue molecular subtypes. (cns.org)
  • Microfluidic genetic profiling of BTICs at the single-cell level was performed to characterize intra-tumoral BTIC heterogeneity. (cns.org)
  • This is the first demonstration of cancer stem cell heterogeneity in glioblastoma at the single-cell level, and particularly as it relates to prospective isolation and bulk tumor subtypes. (cns.org)
  • As a new type of probe, the heavy isotope of water has been widely used in the study of cell development, metabolism, tissue homeostasis, aging, and tumor heterogeneity. (medsci.org)
  • Brain tumor-initiating cells (BTICs), self-renewing multi-potent cells critical for tumor maintenance and growth, are attractive targets of glioblastoma therapy. (cns.org)
  • Fresh human glioblastoma tumors obtained directly from neurosurgical resections were immediately dissociated and passaged in neural stem cell media to enrich the subpopulation of tumor cells with stem-like properties. (cns.org)
  • Two distinct subsets of BTICs were identified co-existing in human glioblastoma, both in de novo tumors and in recurrent tumors after initial complete surgical resection and chemoradiation. (cns.org)
  • Identification of cell surface markers of distinct BTIC subpopulations will support future study of glioblastoma cancer stem cells, and the potential development of BTIC subpopulation-specific therapeutic strategies. (cns.org)
  • Glioblastoma is the most common form of primary brain tumour. (bmj.com)
  • Glioblastoma (GBM) is the most common and aggressive type of primary brain tumor 1 . (nature.com)
  • Glioblastoma is the most aggressive primary brain tumor in adults. (lu.se)
  • We addressed how treatments and hypoxia affect tumor-associated astrocytes in ways that consequently alter glioma cell properties, and how hypoxia and pseudo-hypoxia are involved in stemness maintenance in glioblastoma. (lu.se)
  • For this work, we used genetically engineered mouse models of glioma, primary stromal and glioma cell lines, classical glioblastoma cell lines, and organotypic slice cultures. (lu.se)
  • BMP2 sensitizes glioblastoma stem-like cells to Temozolomide by affecting HIF-1? (omicsdi.org)
  • A nanoparticle carrying the p53 gene targets tumors including cancer stem cells, sensitizes glioblastoma to chemotherapy and improves survival. (omicsdi.org)
  • Glioblastoma multiforme (GBM) is one of the most hypoxic tumors of the central nervous system. (omicsdi.org)
  • Response of primary glioblastoma cells to therapy is patient specific and independent of cancer stem cell phenotype. (omicsdi.org)
  • Glioblastoma multiforme (GBM) contains a population of cells that exhibit stem cell phenotypes. (omicsdi.org)
  • Glioblastoma may be the most common and lethal major human brain tumor. (irjs.info)
  • Launch Glioblastoma multiforme (GBM) can be a devastating human brain tumor with the average success time from medical diagnosis of 12C14 a few months [1]. (irjs.info)
  • Our first trainee focused on the impact of pseudouridine in coding and noncoding RNAs as an effector of glioblastoma stem cells (GSC) and the development of glioblastoma multiforme brain tumors. (ca.gov)
  • Following verification of a stem-like signature (upregulated expression of stemness markers, resistance to chemotherapy, increased spheroid formation, and tumorigenesis capabilities despite inoculation of a small number of cells), we analyzed the interaction of these cells with macrophages via direct and indirect coculture assays. (springer.com)
  • Here we investigated the characteristics of a population of pancreatic cancer tumorspheres derived from different human pancreatic cancer cell lines and a primary line from a genetically engineered KPC mouse model, using flow cytometry and western blotting to analyse surface and stemness markers. (lu.se)
  • Pancreatic cancer tumorspheres display a heterogeneous pattern of surface and stemness markers, nevertheless they are characterised by an increased tumorigenic potential and higher chemoresistance. (lu.se)
  • The objective of this article was to evaluate how these chemotherapeutic regimens affect the proportion of cancer stem cells and the expression of stemness markers. (isciii.es)
  • Tanshinone IIA attenuated the stemness of breast cancer cells, evident by downregulating the expression of stemness markers, hindering the capacity of spheroid formation, decreasing the CD24 − /CD44 + sub-population in a concentration-dependent manner and reducing the tumor-initiating ability of breast cancer cells. (biomedcentral.com)
  • Tumorigenicity ability was evaluated by colony and sphere formation assay, the protein expression of stemness markers and tumor formation in nude mice. (biomedcentral.com)
  • More importantly, deficient-autophagy decreased the protein expression of stemness markers and caused the disappearance of the superiority in tumorigenicity in vitro and vivo in CD271+ OS cells. (biomedcentral.com)
  • There are various types of tumors in breast cancers, which also possess different characteristics, clinical outcomes, and therapeutic responses. (hindawi.com)
  • Using xenograft limiting dilution assays and serial tumor initiation assays, we show that CD117+ cells represent a CSC population. (biorxiv.org)
  • Flow cytometry was utilized to determine the stem cell marker CD44 expression status in GCSCs. (bvsalud.org)
  • CD44 isoforms are heterogeneously expressed in breast cancer and correlate with tumor subtypes and cancer stem cell markers. (lu.se)
  • We used q-RT-PCR and exon-exon spanning assays to analyze the expression of four alternatively spliced CD44 isoforms as well as the total expression of CD44 in 187 breast tumors and 13 cell lines. (lu.se)
  • Breast cancer cell lines showed a heterogeneous expression pattern of the CD44 isoforms, which shifted considerably when cells were grown as mammospheres. (lu.se)
  • Tumors characterized as positive for the CD44+/CD24- phenotype by immunohistochemistry were associated to all isoforms except the CD44 standard (CD44S) isoform, which lacks all variant exons. (lu.se)
  • Unsupervised hierarchical cluster analysis of CD44 isoform expression data divided tumors into four main clusters, which showed significant correlations to molecular subtypes and differences in 10-year overall survival. (lu.se)
  • We demonstrate that individual CD44 isoforms can be associated to different breast cancer subtypes and clinical markers such as HER2, ER and PgR, which suggests involvement of CD44 splice variants in specific oncogenic signaling pathways. (lu.se)
  • Metformin was preferentially cytotoxic to CD44 high /CD24 low cells of MCF-7 cells and, CD44 high /CD24 high cells of MIA PaCa-2 cells, which are known to be cancer stem cells of MCF-7 cells and MIA PaCa-2 cells, respectively. (stemcellsciencenews.com)
  • Researchers report that loss of Wnt-1-induced signaling protein-2 (WISP2) in MCF7 breast cancer cells can also promote the emergence of a cancer stem-like cell phenotype characterized by high expression of CD44, increased aldehyde dehydrogenase activity and mammosphere formation. (stemcellsciencenews.com)
  • We showed that the generation of the cleaved form of the cell surface glycoprotein CD44 leads to the stabilization of the HIFs in the perivascular and the perinecrotic glioma niche, leading to increased hypoxic signaling and glioma cell stemness. (lu.se)
  • After analyzing different tumor stem cell markers (SOX2, OCT4, CD133, CD44 and CD24) in pancreatic cancer cells treated with different chemotherapeutic protocols by means of RT-qPCR, Oxaliplatin and Gemcitabine in monotherapy were the chemotherapies that selected the most cancer stem cells while the FOLFIRI protocol decreased them. (isciii.es)
  • Tras analizar mediante RT-qPCR diferentes marcadores de células madre tumorales (SOX2, OCT4, CD133, CD44 y CD24) en células de cáncer de páncreas tratadas con diferentes protocolos quimioterapéuticos, el Oxaliplatino y la Gemcitabina en monoterapia fueron los quimioterápicos que seleccionaron en mayor medida las células madre cancerosas mientras que el protocolo FOLFIRI las disminuyó. (isciii.es)
  • In vitro mammary spheroid formation, flow cytometry assay on CD24 − /CD44 + sub-population, ALDH activity detection, cell viability assay and western blot analysis, and in vivo tumor-initiating analysis were performed to examine the effects of Tanshinone IIA on the stemness of breast cancer cells. (biomedcentral.com)
  • Le pourcentage de CD44 dans les lymphocytes T périphériques était significativement plus élevé chez les patients que chez les témoins, comme détecté par la cytométrie en flux. (who.int)
  • Disialoganglioside GD2 expression in solid tumors and role as a target for cancer therapy[J]. Front Oncol, 2020, 10. (magtechjournal.com)
  • Further, several other groups have identified them in various solid tumors: brain (Singh et al. (docksci.com)
  • Her research interest focuses on the molecular oncology and immunotherapies of solid tumors and she published more than 40 peer reviewed papers. (stanford.edu)
  • In the past decade, many clinical trials have been investigating anti-CTLA-4 as well as anti-programmed cell death protein 1 (PD-1) therapies in various solid tumors, including HCC. (stanford.edu)
  • Objective: This systemic review and meta-analysis focuses on the prognostic value of p53 Abs expressed in the serum of patients with solid tumors. (stanford.edu)
  • Main Outcome(s) and Measure(s): The primary study outcome was to determine the prognostic value of p53 Abs from a large population of patients with solid tumors, as determined before data collection. (stanford.edu)
  • CTCs-derived organoids (CTCDOs) were characterized through proteome profiling, immunohistochemistry, immunofluorescence, flow cytometry, tumor-forming capacity and drug screening assays. (biomedcentral.com)
  • Mechanistically, stemness is induced in non-stem cancer cells upon their direct contact with macrophages via Notch-Jagged signaling. (nature.com)
  • In breast cancers from patients, the density of TMEM doorways correlates with the proportion of cancer cells expressing stem cell markers, indicating that in human breast cancer TMEM doorways are not only cancer cell intravasation portals but also CSC programming sites. (nature.com)
  • According to the "stochastic model" (or "clonal evolution model") every cancer cell in a tumor could gain the ability to self-renew and differentiate to the numerous and heterogeneous lineages of cancer cells that compromise a tumor These mutations could progressively accumulate and enhance the resistance and fitness of cells that allow them to outcompete other tumor cells, better known as the somatic evolution model. (wikipedia.org)
  • Here, we employ a fluorescent reporter system to track and isolate stem-like cancer cells of cholangiocarcinoma cell lines. (springer.com)
  • Furthermore, cancer cells undergoing EMT phenotypic changes also acquire drug resistance [ 7 ]. (techscience.com)
  • In the cancer stem cell model of tumors, there is a small subset of cancer cells, the cancer stem cells, which constitute a reservoir of self-sustaining cells with the exclusive ability to self-renew and maintain the tumor. (aacrjournals.org)
  • These cancer stem cells have the capacity to both divide and expand the cancer stem cell pool and to differentiate into the heterogeneous nontumorigenic cancer cell types that in most cases appear to constitute the bulk of the cancer cells within the tumor. (aacrjournals.org)
  • To determine how CD117 expression and activation by its ligand stem cell factor (SCF, kit ligand, steel factor) alter prostate cancer aggressiveness, we used LNCaP-C4-2 and PC3-mm human prostate cancer cells, which contain a CD117+ subpopulation. (biorxiv.org)
  • Cancer cells use vitamins quicker than regular cells which may result in an extreme quantity of byproducts within the blood. (apklas.com)
  • We have established an experimental model of CTCs-derived organoids (CTCDOs) obtained from circulating cancer cells spontaneously generated in an orthotopic CRC xenograft model derived from one CRC patient. (biomedcentral.com)
  • A minor subpopulation of cancer cells, known as glioma stem-like cells (GSCs), are thought to play a major role in tumor relapse due to their stem cell-like properties, their high resistance to conventional treatments and their high invasion capacity. (nature.com)
  • Specific areas of interest include understanding the mechanisms, which allow cancer cells to develop resistance to chemotherapy at the cellular and molecular levels. (sc.edu)
  • Transmission electron microscopy and energy-dispersive X-ray microanalysis confirmed the presence of Al specifically in intracytoplasmic electrondense nanodeposits adjacent to mitochondria of colon cancer cells. (bvsalud.org)
  • Al-positive samples showed a significant increase in both the number of vimentin-positive and nuclear ß-catenin-positive cancer cells compared to Al-negative samples. (bvsalud.org)
  • Moreover, the cancer cells themselves are highly plastic, with some of the cells exhibiting stem-like phenotypes. (lu.se)
  • Our data showed that knockdown of HuR in pancreatic cancer cells significantly inhibited cell migration, invasion, and stem-like features. (ku.edu)
  • Restoration of HuR in HuR deleted (KO) pancreatic cancer cells enhanced migration and enriched CSC population. (ku.edu)
  • Additionally, Tanshinone IIA enhanced adriamycin sensitivity and attenuated adriamycin resistance of breast cancer cells. (biomedcentral.com)
  • Notably, miR-125b overexpression enhanced the stemness of breast cancer cells, and miR-125b overexpression or STARD13 knockdown impaired the inhibitory effects of Tanshinone IIA on the stemness of breast cancer cells. (biomedcentral.com)
  • As an important stress-response mechanism, autophagy plays crucial role in the tumor formation and drug resistance of cancer cells including osteosarcoma (OS). (biomedcentral.com)
  • In particular, in mice, whose life spans do not exceed two years, tumor relapse is difficult to study. (wikipedia.org)
  • Chemoresistance and frequent tumor relapse have been the major challenges hampering treatment efficacy. (molcells.org)
  • Treatment includes induction chemotherapy to achieve remission and postremission chemotherapy (with or without stem cell transplantation) to avoid relapse. (msdmanuals.com)
  • Indeed, SOX9 knockdown inhibited anchorage-independent cell growth in vitro and lung colonization in vivo in a mouse xenograft model. (cdc.gov)
  • The oncogenicity of different subpopulations in cell line A549 was compared with tumor xenograft model. (magtechjournal.com)
  • Histological examination of tumor tissue substantiated that administering PPI via gavage to HepG2/S xenograft nude mice inhibited tumor growth and significantly reduced tumor size, as evidenced by xenograft experiments involving nude mice. (bvsalud.org)
  • In the HCC cells, activation of the NF-kappa B signaling pathway and IkB phosphorylation was significantly reduced by EIF5B silencing. (techscience.com)
  • Moreover, we showed that p75 NTR signaling is involved in the activation of the hypoxic signaling pathway and is also regulating glioma cell stemness and migration in hypoxia. (lu.se)
  • ALDH1 has a role in early stem cell differentiation through its function in the oxidation of retinol to retinoic acid, proposed to be a strong candidate for breast cancer stem cells. (hindawi.com)
  • Bone morphogenetic proteins (BMPs), and in particular BMP2, are known to promote differentiation and growth inhibition in GBM cells. (omicsdi.org)
  • GBMs are believed to arise from change of neural stem cells (NSCs) or de-differentiation of adult glioma cells with hereditary lesions [12], [13]. (irjs.info)
  • Using ATOs developed from different cell lines the trainee assessed the ability to support CD34+ differentiation. (ca.gov)
  • Within the B-cell and T-cell categories, two subdivisions are recognized: precursor neoplasms, which correspond to the earliest stages of differentiation, and more mature differentiated neoplasms. (medscape.com)
  • Myeloid-related precursor neoplasms derive from precursor cells that have at least one form of myeloid differentiation. (medscape.com)
  • The presence of the characteristic chromosomal translocation always involving the fibroblast growth factor receptor 1 ( FGFR1 ) gene at chromosome 8p11 in both the myeloid and lymphoid malignancies suggests bilineage differentiation from an affected pluripotent stem cell. (medscape.com)
  • First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)
  • These findings suggest a tumor suppressor role for FOXO3a, which could constitute a potential target for cancer treatment. (spandidos-publications.com)
  • Importance: The protein p53 is an unequivocal tumor suppressor that is altered in half of all cancers. (stanford.edu)
  • Tumor suppressor miRNAs are thought as those that lower development or invasion and so are generally down-regulated in GBM. (irjs.info)
  • A defective tumor suppressor gene is believed to result in the development of spiradenomas. (medscape.com)
  • Spiradenomas seem to be caused by a defective tumor suppressor gene. (medscape.com)
  • A second trainee focused on developing a fluorescent marker to allow for the characterization of a CAR-T cell population as it interacts with the targeted brain tumor. (ca.gov)
  • Many cancers re-emerge after treatment, despite the sensitivity of the bulk of tumor cells to treatments. (mdpi.com)
  • Most human prostate cancers are adenocarcinomas, which originate from the epithelial cells that line the glands and ducts of prostate [ 2 ]. (jcancer.org)
  • In our earlier examine we now have demonstrated that a number of most cancers stem cell CSC markers correlated with CSC properties in MM cells. (apklas.com)
  • Identification of cytoplasmic biologic markers in breast cancers, which are proteins that run in the PUKCA/AKT/mTOR pathways, such as PIK3CA, PTEN, pAKT/pS6, metabolites, and aldehyde dehydrogenase 1 (ALDH1), is being widely developed [ 3 ]. (hindawi.com)
  • Small cell lung cancer (SCLC), a poorly differentiated and highly aggressive tumor, constitutes approximately 15% of all lung cancers ( 1 ). (spandidos-publications.com)
  • Multi-omic analyses revealed a higher tumor mutational burden (TMB) in Al-positive colon cancers (n = 2) compared to a control cohort (n = 100). (bvsalud.org)
  • Some miRNAs which have been examined in GBM and various other cancers attended to become characterized as oncogenic, or in a position to get tumor development through mechanisms such as for example treatment resistance, get away from the disease fighting capability or dys-regulation of angiogenesis. (irjs.info)
  • reliant networks into individual cancer biology To be able to translate the noticed findings to individual cancers biology we integrated our personal of differentially controlled genes in dependence of Dkk2 knock out with genuine FAS human tumors. (pkc-inhibitor.com)
  • Our in vitro studies included scratch wound-healing migration assays and transwell assays to examine PPI's effect on HCC cell migration and invasion. (bvsalud.org)
  • Improved understanding of the underlying cancer biology of this subpopulation of cancer stem cells, thought to drive tumor formation and therapeutic resistance, could potentially lead to improved targeted therapies that prolong patient survival. (cns.org)
  • 5]Nassar D, Blanpain C. Cancer stem cells: basic concepts and therapeutic implications[J]. Annu Rev Pathol, 2016, 11: 47-76. (magtechjournal.com)
  • Taken collectively our information counsel that SP CD9 CD24 and CD26 are CSC markers of MM and might be used as novel therapeutic targets. (apklas.com)
  • Polyphyllin I (PPI), a promising pharmaceutical candidate, has shown potential therapeutic advantages in the treatment of sorafenib-resistant hepatocellular carcinoma (SR-HCC cells). (bvsalud.org)
  • The current require of cancer stem cell (CSC) markers that are quickly evaluated simply by blood samples prevents the establishment of fresh therapeutic strategies in hepatocellular carcinoma (HCC). (monossabios.com)
  • Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)
  • We tested the hypothesis that the stromal cells from PZ and transitional zone (TZ) have differential effects on the ability of tumorigenesis. (jcancer.org)
  • The C-Kit inhibitor, imatinib mesylate, was administrated to confirm the effect of stromal cells on the tumorigenesis. (jcancer.org)
  • Ganglioside GD2 identifies breast cancer stem cells and promotes tumorigenesis[J]. J Clin Invest, 2012, 122: 2066-2078. (magtechjournal.com)
  • This reprogramming phenomenon can be used as one of the markers of tumorigenesis and development. (bvsalud.org)
  • Stromal cells isolated from the PZ and TZ of normal human prostates mixed with DU145 cells subcutaneously injected into athymic nude mice. (jcancer.org)
  • Recent developments in cancer biology have identified the existence of a sub-poplulation of cells - cancer stem cells (CSC) that are resistant to most traditional therapies (e.g. chemotherapy and radiotherapy) and have the ability to repair their damaged DNA. (docksci.com)
  • The aim of this study was to determinate the effect of different chemotherapy protocols used in the clinic on tumor stem cell markers in vitro . (isciii.es)
  • Although surgical resection can be applied when the tumor is well defined (20% of cases) 1 , most patients with pancreatic cancer are treated with chemotherapy. (isciii.es)
  • he current therapy strategy for OS consists of the addition of chemotherapy after surgical removal of tumor, and neoadjuvant chemotherapy followed by surgery. (biomedcentral.com)
  • In this study, we linked SOX9, a stem cell associated transcription factor, to the neoplastic-like properties of human lung epithelial cells chronically exposed to a low-dose of single-walled carbon nanotube s (SWCNTs). (cdc.gov)
  • Furthermore, EIF5B knockdown suppressed epithelial-mesenchymal transition (EMT) and the cancer stem cell (CSC) phenotype. (techscience.com)
  • These effects were partially mediated through enhanced TGF-β signaling, which contributes to tissue fibrosis and epithelial-mesenchymal transition (EMT) of tumor cells. (jci.org)
  • CTCDOs exhibited a hybrid epithelial-mesenchymal transition (EMT) state and an increased expression of stemness-associated markers including the two homeobox transcription factors Goosecoid and Pancreatic Duodenal Homeobox Gene-1 (PDX1), which were also detected in CTCs from CRC patients. (biomedcentral.com)
  • One trainee focused on developing in vitro growth conditions to optimize expansion of luminal epithelial cells and c-KIT+ progenitor cells to explore the early stages of breast cancer. (ca.gov)
  • ABSTRACT Multiple myeloma (MM) is a systemic malignancy of pathologic plasma cells that is treat able with chemotherapeutic agents and irradiation, but rarely curable. (who.int)
  • Cancer stem cells-origins and biomarkers: perspectives for targeted personalized therapies[J]. Front Immunol, 2020, 11: 1280. (magtechjournal.com)
  • A sphere formation assay was performed to evaluate the sphere-forming capabilities of GCSCs. (bvsalud.org)
  • The participants critically discussed the need for a precise definition of cancer stem cells, the requirement for new markers and more rapid and tractable in vitro and in vivo assays, and the need to develop drug screening strategies to selectively target cancer stem cells to generate therapeutics for this subpopulation of cells that could be resistant to classic treatments while possessing potent tumor-forming capacity. (aacrjournals.org)
  • Here, we demonstrate that sublethal doses ionizing radiation specifically promotes the migration and invasiveness of human GSC lines using in vitro and in vivo assays. (nature.com)
  • Disialogangliosides GD2 and GD3 are involved in the attachment of human melanoma and neuroblastoma cells to extracellular matrix proteins[J]. J Cell Biol, 1986, 102: 688-696. (magtechjournal.com)
  • In order to determine whether isolated CSC populations contained antigenic cancer-related mutations, exomic sequencing was performed on tumor cell lines from 5 distinct melanoma tumors using Illumina's sequencing technology. (bmj.com)
  • The expression patterns of stem cell-specific genes of these DU145 cells were examined. (jcancer.org)
  • The autophagy level was evaluated by the mRNA expression of autophagy genes, the protein level of LC3II and p62, and the mean number of GFP-LC3 dot per cell. (biomedcentral.com)
  • tumor cells showed a significant deregulation of stemness genes associated with enhanced colony forming properties. (pkc-inhibitor.com)
  • We therefore investigated the expression of the genes in correlation to individual tumor prognosis and advancement. (pkc-inhibitor.com)
  • Additional screens for MMTV provirus entry sites in tumors revealed several other upregulated genes that are associated with other gene development pathways such as INT2, INT3, and INT4 [4,5,6]. (chemdiv.com)
  • Widespread Mesothelioma Markers The Worldwide Mesothelioma Curiosity Group recognized calretinin cytokeratin 56 WT-1 protein podoplanin and mesothelin as a few of the most helpful mesothelioma markers. (apklas.com)
  • Further examination revealed that PPI hindered the expression of GRP78 protein, resulting in a suppressive effect on SR-HCC cell migration and invasion. (bvsalud.org)
  • The "molecule" could for instance be a cancer cell or a viral protein. (igem.org)
  • And a recent study demonstrates that Tanshinone IIA restrains migratory and invasive ability of cervix carcinoma stem cells by suppressing the transcriptional activity of YAP (Yes-associated protein) [ 11 ]. (biomedcentral.com)
  • The study has previously revealed that miR-125b faciliates cell migratory ability breast cancer via targeting STARD13 (StAR-related lipid transfer protein 13) [ 20 ]. (biomedcentral.com)
  • Within the field of Hematopoietic Stem Cells one trainee explored the impact of SGF29, a protein that recognizes methylated histones and modulates transcription, in leukemic cancer stem cells. (ca.gov)
  • The majority of the biologic markers that are proven to have an association with TNBC resistance against therapy are found in the cytoplasm of TNBC cells. (hindawi.com)
  • Spheroid cells that developed in culture expressed high levels of putative stem cell markers, and showed stronger anchorage-independent growth abilities and resistance to conventional chemotherapeutic drugs compared with the initial monolayer adherent cells. (hilarispublisher.com)
  • Existing cancer treatments have mostly been developed based on animal models, where therapies able to promote tumor shrinkage were deemed effective. (wikipedia.org)
  • If the cancer stem cell model is correct and if such cells retain the hallmarks of some tissue stem cells in being rare and entering the cell cycle infrequently, they could constitute a population that is intrinsically resistant to current therapies designed to kill cycling cells. (aacrjournals.org)
  • If cancer stem cells are relatively refractory to therapies that have been developed to eradicate the rapidly dividing cells within the tumor that constitute the majority of the nonstem cell component of tumors, then they are unlikely to be curative and relapses would be expected. (aacrjournals.org)
  • Despite treatment, tumors invariably recur, and the recurring tumor is resistant to therapies. (lu.se)
  • The data presented in this thesis may serve as valuable resources to help optimize future cell replacement therapies for patients suffering from PD. (lu.se)
  • In both 2D and 3D cultures, stemness marker gene expression is higher in CD117+ cells. (biorxiv.org)
  • The stem cell markers examined in each parental cells and MPeMSCs present similarities of their expression aside from the MYC oncogene and this consistency in gene expression signifies that parental cells even have. (apklas.com)
  • Additionally we examined the expression of the autophagy-related gene complex p62/SQSTM1 and LC-3 in MG63 cells exposed to various concentrations of rapamycin (ranging from 0.4 to 50 μM) for 24 h (Fig. 3A). (exposed-skin-care.net)
  • This analysis led to the discovery of a population of preadipocyte cells whose gene expression is remodeled in an age-specific fashion. (ca.gov)
  • Specifically, the trainee looked at the ability of an endothelial cell AGO 1 gene knockout to rescue streptozotocin-induced damage in female mice. (ca.gov)
  • Understanding the activity of this gene will allow for an improved understanding of how cells can survive DNA damage leading to genomic rearrangements that support the initial stages of tumorgenesis and subsequent cancer development. (ca.gov)
  • Our final student worked to introduce a "suicide gene" construct into engineered in iPSCs for the generation of the insulin-producing -cell to treat type 1 diabetes patients. (ca.gov)
  • Our data have provided evidence for a comparable prediction of clinical outcome in CMF-treated breast cancer patients using conventional clinical variables and gene expression based markers. (lu.se)
  • In highly heterogeneous tumours, like pancreatic ductal adenocarcinoma (PDAC) however, the characterisation of cancer stem cells has led to controversial results due to the lack of consensus on specific markers. (lu.se)
  • Cutaneous T-cell lymphoma (CTCL) (see the image below) is a heterogeneous group of lymphoproliferative disorders characterized by localization of neoplastic T lymphocytes to the skin, with no evidence of extracutaneous disease at the time of diagnosis. (medscape.com)
  • In the glioma cells, EIF5B promotes cell survival by enhancing the translation of several IRES-containing mRNAs including those encoding anti-apoptotic proteins such as XIAP and Bcl-xL [ 4 ]. (techscience.com)
  • Our results highlight the crucial role of ETP as an obesity-associated factor that promotes tumor growth in the context of adipocyte interactions with tumor and stromal cells. (jci.org)
  • MSI1 inhibition may radiosensitize tumors, prevent CSC-positive selection induced by RT, and reduce tumor invasion. (tmu.edu.tw)
  • Consistently, knockdown of HuR led to the inhibition of EMT by upregulating epithelium marker Claudin-1 and downregulating mesenchymal marker Vimentin and Snail. (ku.edu)
  • However, whether casticin inhibits in vitro carcinogenesis and cancer stem cell (CSC) characteristics, and whether casticin activates FoxO3a in small cell lung cancer (SCLC) cells remain unclear. (spandidos-publications.com)
  • The radioresistance of GBM cells relies on the efficient activation of the DNA damage response (DDR), but the mechanisms linking this response with stem-cell status and tumor invasion remain unclear. (tmu.edu.tw)
  • Tanshinone II A is an effective component extracted from Salvia miltiorrhiza and the roles of Tanshinone IIA in regulating the stemness of tumor cells remain unclear. (biomedcentral.com)
  • Collagen VI (COL6) is highly expressed in adipose tissue, upregulated in the obese state, and enriched in breast cancer lesions and is a stimulator of mammary tumor growth. (jci.org)
  • Hence, the adipocyte, as a major constituent of the mammary tumor stroma ( 3 ), is a likely contributor to tumor growth. (jci.org)
  • Scientists showed that MMTV-Her2/Neu mammary tumor cells cultured as non-adherent spheres or as adherent monolayer cells select for stabilizing mutations in p53 that "immortalize" the cultures, and that after serial passages, sphere conditions maintain tumor-initiating cells whereas monolayer cells gradually lose these tumorigenic cells. (stemcellsciencenews.com)
  • In addition, RT-qPCR and Western blot experiments were employed to investigate the tumor stem cell markers OCT4 and SOX2 expression levels in GCSCs. (bvsalud.org)
  • The volume and weight of tumors were significantly higher in mice transplanted with DU145 and stromal cells from PZ. (jcancer.org)
  • Aldehyde dehydrogenase ALDH exercise was validated as a CSC marker in mesothelioma by evaluation of tumor-initiating functionality in mice. (apklas.com)
  • Since then they were systematically cultured as tumorospheres in defined stem cell culture conditions, allowing them to keep their GSC properties including their capacity to generate intracerebral tumors in immunodeficient mice (Supplementary Fig. S1 A). (nature.com)
  • Here, we show that DRAM2 may act as an oncogenic regulator in non-small cell lung cancer (NSCLC). (cancerindex.org)
  • Musashi-1 (MSI1) is a neural stem-cell marker and key oncogenic factor of GBM. (tmu.edu.tw)
  • miRNAs which have become realized as oncogenic or tumor-driving, using their appearance usually elevated in GBM, consist of but aren't limited by miR-21, the miR cluster 17C92, miR-26a, and miRs-221 and -222 [29]C[33]. (irjs.info)
  • Nussе and Varmus identified components of the Wnt/β-catenin pathway in 1982 in a study of oncogenic breast tumor viral diseases (MMTV) [1]. (chemdiv.com)
  • The theory suggests that conventional chemotherapies kill differentiated or differentiating cells, which form the bulk of the tumor but do not generate new cells. (wikipedia.org)
  • Each BTIC subtype was characterized by distinct surface markers, and single-cell molecular profiles relating to distinct bulk tissue molecular subtypes. (cns.org)
  • However, post-mortem analysis of transplanted tissue revealed accumulation of pathological Lewy bodies in a small subset of transplanted cells over time, revealing a host-to-graft disease propagation. (lu.se)
  • A total of 54 of 55 patients treated at RSCM were tested for the expression of ALDH1 through an immunohistochemistry assay of breast cancer tissue using ALDH1 staining. (hindawi.com)
  • Hematoxylin and eosin (HE) staining of tumor tissue specimens, along with immunohistochemistry (IHC), were conducted to evaluate the expression levels of Ki67, GRP78, N-cadherin, Vimentin, and ABCG2. (bvsalud.org)
  • Astrocytes became reactive in response to these cues and produced extracellular matrix that altered glioma cell properties, including stemness. (lu.se)
  • There is certainly accumulating evidence that this glioma cell-of-origin is usually a standard stem or precursor cell from your sub-ventricular zone. (irjs.info)
  • Compact disc133 may possibly not be a common marker for GSCs, as well as the validity of Compact disc133 like a cell-surface molecule indicating glioma cells with stem-like properties must be established for every individual tumor. (irjs.info)
  • In contrast, the data was significantly lower with DU145 and stromal cells from TZ than DU145 alone. (jcancer.org)
  • The purified DU145 cells isolated from the tumors with DU145 and stromal cells in PZ had increased ability to migrate and proliferate, and had increased expression of C-Kit. (jcancer.org)
  • These effects of the stromal cells in the PZ on DU145 cells could be blocked using imatinib mesylate. (jcancer.org)
  • in contrast, the stromal cells in the TZ inhibit it through down-regulating the expression of C-Kit. (jcancer.org)
  • Within the cancer population of the tumors there are cancer stem cells (CSC) that are tumorigenic cells and are biologically distinct from other subpopulations They have two defining features: their long-term ability to self-renew and their capacity to differentiate into progeny that is non-tumorigenic but still contributes to the growth of the tumor. (wikipedia.org)
  • MPeMSCs are multipotent differentiate into neuronal vascular and adipose progeny upon outlined induction and the differentiating cells specific lineage-specific markers equivalent to TUBB3 an early neuronal marker. (apklas.com)
  • In mammary gland development, Notch-dependent heterotypic signaling between resident tissue macrophages and mammary stem cells supports survival and function of the normal mammary stem cell 20 . (nature.com)
  • City of Hope's Train, Educate and Accelerate Mastery of Stem cell research (TEAMS) program was built around four key areas of regenerative medicine research: Cancer Stem Cells, Hematopoietic Stem Cells, Tissue-Specific Stem Cells and Human Pluripotent Stem Cells. (ca.gov)
  • Four trainees worked within the field of Tissue-Specific Stem Cells. (ca.gov)
  • Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)
  • Tumor specimens from 259 NSCLC patients were collected and analyzed. (cancerindex.org)
  • of the initial samples, aswell as individual GBM stem cell and set up cell lines and several individual specimens. (irjs.info)
  • Wnt/β-catenin signaling is a branch of a functional network that is involved in a broad range of biological systems including stem cells, embryonic development and adult organs. (chemdiv.com)
  • These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)
  • With the field of Human Pluripotent Stem Cells we supported four trainees. (ca.gov)
  • Similarly, destruction of neonatally abundant pluripotent stem cells would likely have a more pervasive outcome than destruction of The value of incorporating immunologic appeared more severe and/or persistent when single lineages or differentiated cells that pre- data for the toxicologic assessment of drugs, the exposure occurred perinatally when com- dominate in adults. (cdc.gov)
  • Surface markers of distinct BTIC subpopulations were validated in fluorescence-activated cell sorting (FACS) and colony forming assays. (cns.org)
  • As well as CD9 and CD24 cells have greater potential to generate spheroid colony than adverse cells within the stem cell medium. (apklas.com)
  • We here demonstrated that casticin decreased sphere‑ and colony‑formation capabilities, and downregulated uPAR and CD133 in second‑generation spheres, which were considered as lung cancer stem‑like cells (LCSLCs), from SCLC H446 cells, in a concentration‑dependent manner. (spandidos-publications.com)
  • Colony forming assay Colony forming assays of cells isolated from Dkk2? (pkc-inhibitor.com)
  • cells have a very superior colony development on both regular (43 colonies from Dkk2? (pkc-inhibitor.com)
  • cells demonstrated a significant upsurge in colony developing device on both collagen covered -CFU COL1- (37 Dkk2? (pkc-inhibitor.com)