Markers 1998 analysis tandem repeat sequences. Medical search. Frequent questions

Seidl C , Jäger O, M Kilp, Seifried E. Automated fluorescence PCR based analysis of the STR polymorphism at locus D8S639 and at the CYP19 gene. (lfa-frankfurt.de)
Seidl C , Jäger O, Seifried E . Analysis of the short tandem repeat polymorphism D21S11 in german caucasians. (lfa-frankfurt.de)
Seidl C , Jäger O, Seifried E . Population and family studies of the sequence polymorphism at short tandem repeat locus D8S639 reveals complex repeat patterns. (lfa-frankfurt.de)
Seidl C , Müller S, Kilp M, Seifried E . Fluorescence based multiplex analysis of the STR polymorphism FIBRA, VWFA31 and D18S51 in German caucasoid individuals. (lfa-frankfurt.de)
GENETICS AND GENEALOGY: Y Polymorphism and mtDNA Analyses", by Kevin F. Duerinck, Esq. (duerinck.com)
Using the y polymorphism DNA method, genetic markers are used on my y chromosome and on the y chromosome of my presumed cousin with the same surname in Belgium. (duerinck.com)
Actually, no, because the women whom you have traced using the mtDNA analysis were not the ones married to the males you traced in the y polymorphism analysis. (duerinck.com)
Polymorphism at the tetranucleotide repeat locus DYS389 in 10 populations reveals strong geographic clustering. (geneticsmr.com)
A 40-bp variable number of tandem repeats (VNTR) polymorphism exists in the 15th exon of DAT1 , the gene encoding the human dopamine transporter (DAT). (biomedcentral.com)
Flp recombinase, the enzymatic basis of the system, is a Saccharomyces cerevisiae derived enzyme that utilizes a substrate Flp recombination target (FRT) sequence upstream of a gene of interest (GOI) to site-specifically insert the GOI into a target site in a host cell line, thereby providing a well-controlled approach to establishing the in vitro functional effect of the DAT1 VNTR polymorphism on the DAT [ 37 ]. (biomedcentral.com)

The increasing accessibility of whole-genome sequencing across research and clinical settings has improved our ability to predict antibacterial susceptibility, to track epidemics at the level of individual outbreaks and wider historical trends, to query the efficacy of the bacille Calmette-Guérin (BCG) vaccine, and to uncover targets for novel antitubercular therapeutics. (jci.org)
Whole-genome sequencing (WGS) of M. tuberculosis and related mycobacteria is now routine, allowing comparisons across time and space. (jci.org)
To estimate the genome-wide pattern of mutations at STR loci, we analyze blood-derived whole-genome sequencing data for 544 individuals from 29 three-generation CEPH pedigrees. (biomedcentral.com)
Use whole genome sequencing to study how and why certain fungi spread and make people sick. (cdc.gov)

Since 2014 sixteen loci of the DNA-17 system are analysed, resulting in a string of 32 numbers, being two allele repeats from each of the sixteen loci. (wikipedia.org)
Scotland has used 21 STR loci, two Y-DNA markers and the gender identifier since 2014. (wikipedia.org)
Using a population-based ever, these analyses are limited to those sites that vary approach that analyzes multiple loci around the chromo- between the compared genomes and are potentially mis- some, we demonstrate that neutral genetic variation in leading (13-15). (cdc.gov)
Seifried E, Seidl C , Grigorean R, Brude R, Jäger O, Langenbeck U, Zang K.D. A rare paternity case with PP 99,99% and exclusion at three loci derived from the 33.15 VNTR sequence. (lfa-frankfurt.de)
Seidl C , Müller S, Jäger O, Seifried E . Sequence analysis and population data of short tandem repeat polymorphisms at loci D8S639 and D11S488. (lfa-frankfurt.de)
2) at qtl and marker loci. (liverpool.ac.uk)
Loci were isolated from a genomic library enriched for AC repeats. (researchgate.net)
Short tandem repeats (STRs) compose approximately 3% of the genome, and mutations at STR loci have been linked to dozens of human diseases including amyotrophic lateral sclerosis, Friedreich ataxia, Huntington disease, and fragile X syndrome. (biomedcentral.com)
Due to the repetitive structure of STRs and their high mutability, sequencing and genotyping these loci is difficult, especially using short-read sequencing data. (biomedcentral.com)
Some of these tools are designed to detect STR expansions at disease-related loci, while others detect expansions and contractions of STRs genome-wide but are constrained by sequencing read length and the STR motif size. (biomedcentral.com)
2004). Characterization and haplotype analysis of 10 novel Y-STR loci in Chinese Han population. (geneticsmr.com)
2009). Decreased rate of evolution in Y chromosome STR loci of increased size of the repeat unit. (geneticsmr.com)
Variation in short tandem repeat sequences - a survey of twelve microsatellite loci for use as forensic identification markers. (geneticsmr.com)
The first objective of this study was to generate a linkage map of D. magna based on 109 VNTR marker loci. (biomedcentral.com)

The variable number of tandem repeats (VNTR) typing method was employed, as previously described, to further investigate these strains ( 11 ). (cdc.gov)
Variable numbers of tandem repeats (VNTR) or microsatellite markers, on the other hand, are codominant markers and therefore provide more information. (biomedcentral.com)
Though the VNTR resides in a region encoding the 3' untranslated region (UTR) and does not alter the protein's amino acid sequence, the prevalent 10-repeat variant has shown both linkage and association to Attention Deficit Hyperactivity Disorder (ADHD). (biomedcentral.com)
Though the VNTR resides in the 3'UTR and therefore does not affect the protein's amino acid sequence, regulatory factors such as mRNA stability and nuclear transport, and protein synthesis are potentially regulated by such variations [ 11 - 14 ]. (biomedcentral.com)
Despite its high frequency in the general population [ 7 ] and an absence of studies addressing possible effects of the VNTR on measures of DAT physiology and pharmacology, the 10-repeat DAT1 allele has become generally recognized as a "high risk" allele for ADHD. (biomedcentral.com)

To accomplish this, we developed a marker panel densely covering the 1.4 Mb DYX2 locus and assessed association with reading, language, and IQ measures in subjects from the Avon Longitudinal Study of Parents and Children. (springer.com)
The complete sequences of the galago and rabbit beta-globin locus control regions: extended sequence and functional conservation outside the cores of DNase hypersensitive sites. (shengsci.com)
The locus control region (LCR) of mammalian beta-globin genes covers at least 17 kb at the 5' end of the gene cluster and has been implicated in chromatin domain opening, enhancement, and insulation from neighboring sequences. (shengsci.com)
Repeat structure of DYS389 locus. (geneticsmr.com)

The second method is called mitochondrial DNA, or mtDNA analysis. (duerinck.com)
Now what if you had also done a mtDNA analysis? (duerinck.com)
1999). Sex-specific migration patterns in Central Asian populations, revealed by analysis of Y-chromosome short tandem repeats and mtDNA. (geneticsmr.com)
The divergence between D. pulex and D. magna had been estimated from mtDNA sequences to be about 200 MYA [ 10 ]. (biomedcentral.com)

To better understand the karyotype organization in Melipona and the relationship among the subgenera, we mapped repetitive sequences and analyzed previously reported cytogenetic data with the aim to identify cytogenetic markers to be used for investigating the phylogenetic relationships and chromosome evolution in the genus. (karger.com)
Approximately 55% of the BES reads (97,846) had more than 100 base pairs of contiguous non-repetitive sequences. (biomedcentral.com)
A local high frequency of microhomologies, macrohomologies, tandem repeats, and repeat/repetitive sequences contribute to a high rate of nonallelic homologous recombination involving NEMO , resulting in the development of de novo deletion mutations. (medscape.com)

U.K. clinical isolates, silent single nucleotide polymor- variation in insertion sequences (4) and repetitive genom- phisms (sSNPs) resolve an unambiguous phylogeny and ic elements (5,6), have elucidated clusters of identical and provide a unifying framework for epidemiologic, popula- closely related strain families (7-9). (cdc.gov)
7- 9 All reported mutations are single nucleotide alterations leading to splice site, missense, nonsense, or frameshift mutations, or intra-exonic deletions and insertions of up to four nucleotides, which have been detected through exonic PCR amplifications and sequencing. (bmj.com)

Microsatellites were observed only in euchromatin regions, whereas the (TTAGG) 6 repeats were found at telomeric sites in both groups. (karger.com)
In genetic diversity studies, the most frequently used markers are microsatellites. (intechopen.com)
Short tandem repeats (STRs), or microsatellites, are 1-6 base pair (bp) motifs of repeating units of DNA. (biomedcentral.com)

We find that this type of non-canonical fork convergence in fission yeast is prone to trigger deletions between repetitive DNA sequences via a mechanism we call Inter-Fork Strand Annealing (IFSA) that depends on the recombination proteins Rad52, Exo1 and Mus81, and is countered by the FANCM-related DNA helicase Fml1. (elifesciences.org)

Whole exome sequencing revealed a de novo heterozygous missense variant in all three patients in the p.Glu209Lys variant of the PACS2 gene. (bvsalud.org)

The phyloge- high-throughput gene sequencing of structural genes (19) netic structure is concordant with all the previously and host immune system protein targets (20) in M. tuber- described genotypic and phenotypic groupings of M. tuber- culosis strains and provides a unifying framework for both culosis isolates indicated low levels of sequence diversity. (cdc.gov)
genetic analysis of cinnamyl alcohol dehydrogenase in loblolly pine: single gene inheritance, molecular characterization and evolution. (liverpool.ac.uk)
Polymerase chain reaction (PCR) followed by DNA sequencing was performed targeting the rpoT gene of M. leprae . (ilsl.br)
Isolates were classified into two groups based on the number of tandem repeats composed of 6 base pairs in the rpoT gene. (ilsl.br)
El an lisis se hizo por la reacci n en cadena de la polimerasa (PCR) y por secuenciaci n del DNA de la region correspondiente al gene rpo T de M. leprae . (ilsl.br)
Los aislados se clasificaron en dos grupos con base en el n mero de 'secuencias tandem' compuestas por 6 pares de bases en el gene rpo T. Los aislados de Jap n (excepto Okinawa) y Korea correspondieron a un genotipo, mientras que los aislados de los pa ses del sudeste asi tico, Brazil, Haiti, y Okinawa en Japon, correspondieron a un segundo genotipo. (ilsl.br)
The mouse biliary glycoprotein gene (Bgp): partial nucleotide sequence, expression, and chromosomal assignment. (shengsci.com)
Its sequence, when compared to those of members of the human carcinoembryonic antigen gene family, was most homologous to the sequence of the human biliary glycoprotein (BGP) gene. (shengsci.com)
The identification and sequence of KiSS-1 (HGMW-approved symbol, KISS1), a human malignant melanoma metastasis-suppressor gene, was recently published. (shengsci.com)
Wdr12, a mouse gene encoding a novel WD-Repeat Protein with a notchless-like amino-terminal domain. (shengsci.com)
Chromosomes are the carriers of genetic material and chromosome mutations are crucial sources of genetic variation [ 5 ], we can use these mutations as markers to determine the specific location of a gene on the chromosome, and its position relative to other genes. (biomedcentral.com)
TPD1-like Gene as a Suitable Marker for Early Sex Determination in Date Palm (Phoenix dactylifera L. (mdpi.com)

3, 10 However, analyses of SDHC in four series of patients with paraganglioma or pheochromocytoma 6, 8, 11, 12 yielded no definitive SDHC mutations. (bmj.com)

We report information about the chromosome number and morphology, C-banding patterns, and localization of ribosomal DNA clusters and telomeric ( TTAGG ) n repeats. (pensoft.net)

The complete cause drug resistance are distributed throughout the tree, genome sequences (15-18) provide access to all regions of whereas nonsynonymous base substitutions unrelated to the chromosome and facilitate such studies. (cdc.gov)
Southern blot analysis of EcoRI-digested mouse cellular DNA with this probe detected four restriction fragments, all of which appear to be located on mouse chromosome 7, Northern blot analysis using the mouse probe demonstrated that related mRNA species were expressed in some normal adult mouse tissues. (shengsci.com)
2010). US forensic Y-chromosome short tandem repeats database. (geneticsmr.com)
2001). DNA commission of the International Society of Forensic Genetics: recommendations on forensic analysis using Y-chromosome STRs. (geneticsmr.com)

Nucleotide sequences provide robust, genes associated with antimicrobial drug resistance has sufficient variation to construct a robust phylogenetic tree portable, and comparable data for studying population for M. tuberculosis . (cdc.gov)
Our in-depth analyses show that environmental variation has a substantial influence on Tp which demonstrates the necessity of careful experimental design and cautious interpretations of Tp measurements together with a thorough description of the methods and equipment used to conduct behavioral studies. (solamonrenewableenergy.com)

Abstract Ten polymorphic microsatellite markers are described for the Mediterranean bath sponge Spongia officinalis. (researchgate.net)

The mutational processes that generate this vari- with a minimum of four distinct M. tuberculosis lineages, ation are understood, and sequence data have been suc- closely related to M. bovis . (cdc.gov)
the complete genome sequence of Mycobacterium tuberculosis continues to provide an invaluable resource to understand tuberculosis (TB), the leading cause of global infectious disease mortality. (jci.org)
The use of spoligotyping was based on the presence or absence of 43 variable spacers in the direct repeat (DR) region of M. tuberculosis . (cdc.gov)

Although extensive genomic sequencing was performed in populations. (cdc.gov)
In a species whose populations in the eastern Mediterranean basin have been substantially declined due to recurring mass mortality incidents and intensive harvesting, these markers are expected to assist studies of genetic structure and differentiation between populations. (researchgate.net)
2005). The species appears to be semelparous (Gjøsæter and Loeng, 1987), although females from fjord populations may survive to spawn a second time (Christiansen and Siikavuopio, 1998). (scielo.br)

Only patterns of short tandem repeats are stored in the NDNAD - not a person's full genomic sequence. (wikipedia.org)
In this report, we present a corrected genomic sequence, genomic structure, and refined chromosomal location for KiSS-1. (shengsci.com)

The linkage map itself is based on 109 microsatellite markers, which produced ten major linkage groups ranging in size from 31.1 cM to 288.5 cM. (biomedcentral.com)
Here we report on a D. magna linkage study using microsatellite markers. (biomedcentral.com)

The first rainbow trout repeat elements database (INRA RT rep1.0) containing 735 putative repeat elements was developed, and identified almost 59.5% of the BES database in base-pairs as repetitive sequence. (biomedcentral.com)
Identification of putative transmembrane receptor sequences homologous to the calcium-sensing G-protein-coupled receptor. (shengsci.com)

Since the disease is prone to recurrence after surgery, HGF patients must undergo repeated surgical procedures of gingival resection, which brings a heavy psychological and economic burden to HGF patients and their families. (nature.com)

Specific molecular and biochemical markers associated with Fusarium wilt resistance were identified. (ppjonline.org)
The dendrogram based on combined data of molecular and biochemical markers grouped the 16 faba bean genotypes into three clusters. (ppjonline.org)
With the rapid development of modern biotechnology, biochemical markers, such as proteins and isozymes, were utilized. (biomedcentral.com)
They overcome the limitations of morphological, cytological, and biochemical markers, namely the small numbers of such markers and the fact they can be environmentally influenced. (biomedcentral.com)

Again, please understand that the molecular biology techniques used in mitochondrial DNA analysis should not be confused with DNA fingerprinting which is carried out on nuclear DNA. (duerinck.com)

However, the lack of a reference genome sequence hampers research progress for both academic and applied purposes. (biomedcentral.com)
The discovery of specific repeat elements will facilitate analyses of sequence content (e.g. for SNPs discovery and for transcriptome characterization) and future genome sequence assemblies. (biomedcentral.com)
Furthermore, comparative genomics through BES can be used for identifying positional candidate genes from QTL mapping studies, aid in future assembly of a reference genome sequence and elucidating sequence content and complexity in the rainbow trout genome. (biomedcentral.com)
While the genome sequence was already transformative at the time, the past 25 years of progress have substantially increased its impact on TB taxonomy, drug discovery, resistance mechanisms, epidemiology, vaccine development, and pathogenesis. (jci.org)

Les isolats furent class s en deux groupes bas s sur le nombre de motifs r p t s 2 par 2 (tandem repeats) compos s de 6 paires de bases au sein du g ne rpoT. (ilsl.br)

A comparative analysis of the available cytogenetic data for the family Osmeridae reveals diploid numbers higher than 48 and high fundamental numbers. (scielo.br)

Different variants of FISH can be used to retrieve information on genomes from (almost) base pair to whole genomic level, as besides only second and third generation sequencing approaches can do. (frontiersin.org)
those containing only the DAT1 coding region or the coding region flanked by a truncated 3' UTR resulted in greater DAT density than either of the naturalistic 9- and 10-repeat variants. (biomedcentral.com)

After Alu I digestion, the DNA was transferred to a nylon membrane for polymorphic guanine cytosine-rich repetitive sequence (PGRS) Southern blotting. (cdc.gov)

Its principle is based on the use of DNA polymerase which is an in vitro replication of specific DNA sequences. (intechopen.com)

The data held on the NDNAD is owned by the police authority which submitted the sample for analysis. (wikipedia.org)
both studies, comparable sequence data were obtained on a limited number of highly selected isolates. (cdc.gov)
The study of biological complexity is a new frontier that requires high-throughput molecular technology, high speed computer memory, new approaches to data analysis, and the integration of interdisciplinary skills. (intechopen.com)
Build data systems that link various data sources and allow for automated, more efficient data analysis and visualization. (cdc.gov)

At present, DNA molecular marker techniques are widely applied in the fields of germplasm identification, phylogenetics, and genetic structural analysis. (biomedcentral.com)

In this investigation, we aimed to detect some biochemical and/or molecular markers related to resistant genes for FW disease in some genotypes of faba bean. (ppjonline.org)
Dominant markers like AFLPs, RFLPs and RAPDs are fast and provide evidence of the overall genetic architecture, but do not effectively disentangle the exact genes responsible for trait differences [ 14 ]. (biomedcentral.com)

This review will help us better understand the characteristics of different genetic markers and the genetic diversity of animal genetic resources. (biomedcentral.com)
However, sufficient genetic markers for evaluating the population structure and other aspects of available animal genetic resources are necessary to assess genetic diversity. (biomedcentral.com)
All of these DNA-based markers contain specific advantages and have played significant roles in the evaluation of genetic diversity in farm animals. (biomedcentral.com)
such information is much more precise for the assessment of genetic diversity than previous markers. (biomedcentral.com)

In order to enrich the genomic tools already available in this species and provide further insight on the complexity of its genome, we sequenced a large number of rainbow trout BAC-end sequences (BES) and characterized their contents. (biomedcentral.com)
The capelin, Mallotus villosus (Müller, 1776), is a smeltlike fish species widely distributed in sub arctic waters (Gjøsæter, 1998). (scielo.br)

Therefore, these markers can be used in the early selection of resistant genotypes to provide the effort and cost resulting from the use of traditional breeding methods. (ppjonline.org)

These isolates, negative for IS 6110 by RFLP analysis, were from 1,180 Maryland cases. (cdc.gov)

2006). DNA Commission of the International Society of Forensic Genetics (ISFG): an update of the recommendations on the use of Y-STRs in forensic analysis. (geneticsmr.com)

Increased expression of tumor necrosis factor receptor-1 and elevated oxidative stress markers have been reported in a neonate with IP and encephalopathy. (medscape.com)
Renal function indexes, serum inflammatory factors, and tumor markers were evaluated. (biomedcentral.com)
Nursing based on oxygen administration decreased the value of renal function indexes, serum inflammatory factors, and tumor markers in renal cell carcinoma patients. (biomedcentral.com)

The following is a brief summary on the principles and advancements of primary genetic markers involved in assessments of Animal Genetic Resources (AnGR). (biomedcentral.com)

In earlier studies, morphological markers and eco-geographical factors were used to represent diversity, and after that, chromosomal karyotyping was developed. (biomedcentral.com)

Seidl C , Müller S, Seifried E . Sequence analysis of the short tandem repeat polymorphisms D11S488 and D19S246 in the German caucasians. (lfa-frankfurt.de)

Scientific Working Group on DNA Analysis Methods (SWGDAM) Y-STR Subcommittee. (ojp.gov)
2004. Report on the current activities of the Scientific Working Group on DNA Analysis Methods Y-STR Subcommittee. (ojp.gov)

BES analyses identified 6,848 simple sequence repeats (SSRs), of which 3,854 had high quality flanking sequences for PCR primers design. (biomedcentral.com)
Metabolites were quantified using reversed-phase ultra-high performance liquid chromatography and electrospray ionization tandem mass spectrometry. (cdc.gov)

Indeed, if the sequence of interest is present in the DNA extract, it is possible to selectively replicate it (we speak of amplification) in very large numbers. (intechopen.com)
The WD-repeat protein family consists of a large group of structurally related yet functionally diverse proteins found predominantly in eukaryotic cells. (shengsci.com)

Strain-specific markers could provide the necessary tools for understanding this aspect of the epidemiology of leprosy. (ilsl.br)

DNA isolation, genotyping of simple tandem repeat polymorphisms, PCR amplification and sequencing were performed using standard techniques 2 and all simple tandem repeat polymorphisms were amplified in the presence of 10% glycerol and 5% DMSO after labelling one oligonucleotide primer with 32 P and analysed on a 6% polyacrylamide gel. (bmj.com)

A number of markers are now available to detect nuclear DNA polymorphisms. (intechopen.com)
The D. magna linkage map presented here provides extensive coverage of the genome and a given density of markers that enable us to detect QTLs of moderate to strong effects. (biomedcentral.com)

Forensic DNA typing: Biology, technology, and genetics of STR markers. (ojp.gov)
2009). The effects of Asian population substructure on Y STR forensic analyses. (geneticsmr.com)
2005). Separate analysis of DYS385a and b versus conventional DYS385 typing: is there forensic relevance? (geneticsmr.com)

Next-generation sequencing was performed to investigate genomic alterations caused by surgical resection of specimens. (bvsalud.org)

Chemicals and Drugs3

Analytical, Diagnostic and Therapeutic Techniques and Equipment3

Phenomena and Processes13

Information Science3

Polymorphism10

Whole-genome4

Loci14

VNTR5

Locus4

MtDNA4

Repetitive sequences3

Single nucleotide2

Microsatellites3

Deletions1

Exome1

Gene12

Mutations1

TTAGG1

Chromosome4

Variation2

Abstract1

Tuberculosis3

Populations3

Genomic sequence2

Microsatellite markers2

Putative2

Recurrence1

Biochemical markers4

Mitochondrial1

Genome sequence4

Motifs1

Cytogenetic1

Variants2

Polymorphic1

Vitro1

Data4

Identification1

Genes2

Genetic diversity4

Species2

Genotypes1

Isolates1

STRs1

Tumor3

Assessments1

Chromosomal1

Short tandem1

Methods2

High2

Large2

Specific1

Simple1

Detect2

Forensic3

Surgical1