• The following product was used in this experiment: Ki-67 Monoclonal Antibody (SolA15), Brilliant Ultra Violet™ 615, eBioscience™ from Thermo Fisher Scientific, catalog # 366-5698-82, RRID AB_2925428. (thermofisher.com)
  • Cells were then surface-stained with CD45R (B220) Monoclonal Antibody, FITC (Product # 11-0452-82) and stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with 1.0 µg of Ki-67 Monoclonal Antibody, Brilliant Ultra Violet 615. (thermofisher.com)
  • Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein. (thermofisher.com)
  • The SolA15 antibody also recognizes human, non-human primate and canine Ki-67. (thermofisher.com)
  • The following product was used in this experiment: Ki67 Monoclonal Antibody (OTI5D7), TrueMAB™ from Thermo Fisher Scientific, catalog # TA801577. (thermofisher.com)
  • 1. Gerdes J., Schwab U., Lemke H., and Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. (nordicmubio.com)
  • Characterisation of normal peripheral blood cells in cycle identified by monoclonal antibody Ki-67. (bmj.com)
  • METHODS: Single and double immunoenzymatic staining procedures, immunoperoxidase, and immunoalkaline phosphatase were used with Ki-67, a monoclonal antibody that recognises a nuclear antigen present in proliferating cells, with markers expressed in B and T lymphocytes and monocytes. (bmj.com)
  • Monocytes detected by the anti-lysozyme antibody were consistently Ki-67 negative. (bmj.com)
  • The sections were stained with an antibody directed against the Ki-67 antigen. (thejcdp.com)
  • Antigen KI-67 also known as Ki-67 or MKI67 is a protein that in humans is encoded by the MKI67 gene (antigen identified by monoclonal antibody Ki-67). (journalcra.com)
  • The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. (biolegend.com)
  • The three main serologic markers used to determine HBV infection status are hepatitis B surface antigen (HBsAg), antibody to hepatitis B surface antigen (anti-HBs), and antibody to hepatitis B core antigen (anti-HBc) ( Table 1 ). (cdc.gov)
  • and cell differentiation ( SOX2 and TGFB3 ) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. (hindawi.com)
  • 3. Gerdes J., Becker M.H.G., Key G., and Cattoretti G. (1992) Immunohistochemical detection of tumour growth fraction (Ki-67 antigen) in formalin-fixed and routinely processed tissues. (nordicmubio.com)
  • Strategies for improving the immunohistochemical staining of various intranuclear prognostic markers in formalin-paraffin sections: Androgen receptor, estrogen receptor, progesterone receptor, p53 protein, proliferating cell nuclear antigen, and Ki-67 antigen revealed by antigen retrieval techniques. (journal-jmsr.net)
  • A panel of immunohistochemical markers including estrogen receptor ERcarcinoembryonic antigen CEAanti-p16, MUC6 and ki 67 antibodies, and HPV testing throat cancer caused by hpv symptoms important elements in hpv and throat cancer statistics positive diagnosis, but there are cases where the determination of cell phenotype is not sufficient for a certain diagnosis. (bethlen-foundation.ro)
  • This study aimed to combine some markers to establish an immunohistochemical score (MSI-P53-Ki-67[MPK]) and stratify postoperative patients with gastric cancer according to the score. (biomedcentral.com)
  • Immunohistochemical (IHC) examination with antibodies against proliferating cell nuclear antigen (PCNA) and Ki-67 were detected in the basal layer of the epidermis and connective tissue. (ankara.edu.tr)
  • Kawahira K (1999): Immunohistochemical staining of proliferating cell nuclear antigen (PCNA) in malignant and nonmalignant skin diseases. (ankara.edu.tr)
  • We analyzed the immunohistochemical expression of PRAME and Ki-67 in 165 melanocytic lesions, including 92 primary melanomas, 19 metastatic melanomas, and 54 melanocytic nevi using tissue microarrays. (ingentium.com)
  • Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. (thermofisher.com)
  • Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation. (thermofisher.com)
  • It was shown that two proliferation markers, Ki-67 and proliferating cell nuclear antigen (PCNA), are regulated by these factors. (wikipedia.org)
  • 5 , 6 In justification of the reasons above, many biologic markers have been proposed to provide information on the differentiation, proliferation, and prognosis of various lesions. (thejcdp.com)
  • With this view in mind, the present study is designed to elucidate the biological behavior of the cancer cells in OSCC respective to their location, using Ki-67 immunological probe for cellular proliferation. (thejcdp.com)
  • The antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. (journalcra.com)
  • Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. (biolegend.com)
  • The percentage of Ki-67-positive tumor nuclei was used to calculate the proliferation index. (ingentium.com)
  • Ki-67 labeling index (LI) is an important indicator of tumor cell proliferation in glioma, which can only be obtained by postoperative biopsy at present. (biomedcentral.com)
  • Ki-67 is a non-nuclear protein expressed in proliferating cells, which is related to cell proliferation, differentiation, metastasis, and apoptosis. (biomedcentral.com)
  • Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. (thermofisher.com)
  • Monoclonal antibodies directed toward nuclear antigens are increasingly used as tools to obtain valuable information concerning the proliferative changes of the tumors. (thejcdp.com)
  • Background/Aim: To demonstrate the value of Ki-67 in distinguishing between partial and complete hydatidiform moles. (iiarjournals.org)
  • The independent prognostic value of Ki-67 has been studied with variable results. (ingentium.com)
  • A single pathologist evaluated the protein expression of hormone receptors (estrogen (ER) and progesterone receptor (PR)), HER2, Ki67, and GRB7 by immunohistochemistry (IHC). (bvsalud.org)
  • For immunohistochemistry, all meningioma tumors at Elhassan Medical Laboratory for histopathology and cytology during the above mentioned period were processed for ki 67 antigen. (journalcra.com)
  • Several such molecular markers have been extensively studied through immunohistochemistry (IHC), among them the somatostatin receptors type 2 (SSTR-2) and type 5 (SSTR-5), which are known to correlate with response to somatostatin analogues treatment, the SSTR-2 negative tumors usually being resistant to first-generation analogues, while SSTR-5 potentially being a predictive marker for the novel agent, Pasireotide. (rjme.ro)
  • Methods: Using immunohistochemistry, expression levels of p63 and Ki-67 were determined in 16 myoepithelial carcinoma samples and correlated with clinicopathological characteristics and patient prognosis. (cyberleninka.org)
  • Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin). (thermofisher.com)
  • Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. (thermofisher.com)
  • The advanced pathological grade was associated with strong TGFB1, VEGFA, and Ki67 protein expression and absent Tp53 staining. (hindawi.com)
  • 1987). Ki-67 Protein is a nuclear protein, which is ubiquitous present during cell division, that means in all phases of mitosis (S1, G1, G2 and M phase). (nordicmubio.com)
  • Ki-67 nuclear protein. (nordicmubio.com)
  • The Ki-67, a large nonhistone protein with molecular weight of approximately 395 kDa, has been extensively used as a marker to read the proliferative activity during the G1, S, G2, and M phases of the cell cycle. (thejcdp.com)
  • Predictive Role of p53 Protein as a Single Marker or Associated to Ki67 Antigen in Oral Carcinogenesis. (bvsalud.org)
  • Other novel markers, such as the E-cadherin cell-adhesion protein, the aryl hydrocarbon receptor-interacting protein (AIP), the cytoskeleton molecule filamin A (FLNA) and the Ki-67 nuclear antigen have also been the highlight of IHC studies on growth hormone (GH)-producing tumors, with promising results regarding their predictive roles for the outcome of acromegalic patients. (rjme.ro)
  • Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. (biolegend.com)
  • E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa. (biolegend.com)
  • The correlation between low Ki-67 index and histologically low-grade tumors is strong. (thermofisher.com)
  • Hence, Ki-67 antigen-labeled cells can prove to be an effective tool to grade the tumors and finally contribute to the prognosis of the disease. (thejcdp.com)
  • Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors. (biolegend.com)
  • To interpret their results they performed careful anchor gene correlation analysis (AGCA), using the expression of proliferative markers (Ki-67 antigen or PCNA ), inflammatory genes ( MCP -1 or interleukin 6) or myelination genes (such as PMP22 or MAG ), as anchor genes. (the-scientist.com)
  • Hatipoglu F, Ozdemir O, Kıran MM (2009): Detection of argyrophil nucleolar organizer regions (AgNORs) and proliferating cell nuclear antigen (PCNA) in epithelial skin tumours from domestic animals. (ankara.edu.tr)
  • Lu S, Tiekso J, Hietanen S, Syrjaènen K, Havu VK, Syrjaènen S (1999): Expression of cell-cycle proteins p53, p21 (WAF-1), PCNA and Ki-67 in benign, premalignant and malignant skin lesions with implicated HPV involvement. (ankara.edu.tr)
  • CONCLUSIONS: The methodology described is useful for the simultaneous detection of nuclear and cytoplasmic antigens. (bmj.com)
  • Assessing the Prognostic Importance of ER, PR Expression in Meningiomas by Comparing with Proliferative Rate Using Ki67. (journal-jmsr.net)
  • Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. (thermofisher.com)
  • Conclusion: The Ki-67 expression was higher in complete than in partial hydatidiform moles. (iiarjournals.org)
  • In retrospective studies the intensity of staining and the frequency of Ki-67 expression in cell nuclei was interpreted as a prognostic factor for the malignancy of tumours (Gerdes et al. (nordicmubio.com)
  • 9. Iamaroon A, Khemaleelakul U, Pongsiriwet S, Pintong J. Co-expression of p53 and Ki67 and lack of EBV expression in oral squamous cell carcinoma. (bvsalud.org)
  • Expression of p53 in leukoplakia and squamous cell carcinoma of the oral mucosa: correlation with expression of Ki67. (bvsalud.org)
  • 6 ] conducted a comprehensive search of relevant research and used the TCGA dataset and found that positive expression of ki-67 correlated with OS and DFS in osteosarcoma. (biomedcentral.com)
  • In addition, p63 and Ki-67 expression levels were associated with myoepithelial carcinoma recurrence and metastasis. (cyberleninka.org)
  • however, there was no statistically significant difference between Ki-67 expression and survival (P = 0.24). (cyberleninka.org)
  • Conclusion: Expression of ERh is an independent marker for favorable prognosis after adjuvant tamoxifen treatment in ERa-negative breast cancer patients and involves a gene expression program distinct from ERa. (lu.se)
  • Ki-67 is used as a marker for determining the growth fraction of a given population of cells. (thermofisher.com)
  • RESULTS: In the five healthy donors 2.1% (range 1.6-3.7%) cells of the blood mononuclear fraction and 2.7% (range 2.3-3.9%) lymphocytes were Ki-67 positive. (bmj.com)
  • Ki-67 is an excellent marker to determine the growth fraction of a given cell population, the fraction of Ki-67-positive tumor cells (the Ki-67 labeling index) is often correlated with the clinical course of cancer. (journalcra.com)
  • Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). (thermofisher.com)
  • Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. (thermofisher.com)
  • Ki-67 was expressed significantly less in refractory ANLL than initial ANLL and DNA topisomerase IIα was expressed significantly more in the surviving patients group. (karger.com)
  • 2008. Prognostic implications of biologic markers in intracranial meningiomas: 120 Cases. (journal-jmsr.net)
  • This study aimed to explore the correlation between Ki-67 LI and apparent diffusion coefficient (ADC) parameters and to predict the level of Ki-67 LI noninvasively before surgery by multiple MRI characteristics. (biomedcentral.com)
  • There was a negative correlation between ADC parameters and Ki-67 LI, and the multivariate logistic regression model combined with peritumoral edema and ADC parameters could improve the prediction ability of Ki-67 LI. (biomedcentral.com)
  • Conclusions: Recurrence and metastasis in myoepithelial carcinomas are more frequent in p63-positive and Ki-67-positive EMCs, and poor prognosis is associated with overexpression of p63. (cyberleninka.org)
  • Mrs. Berta Kamprad Foundation, John and practice as a marker associated with prognosis and response to Augusta Persson Foundation for Medical Science, and University Hospital of Lund endocrine therapies such as tamoxifen (4). (lu.se)
  • Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. (journalcra.com)
  • The demonstration that a proportion of normal blood lymphocytes are in cell cycle raises the issue of whether immunophenotypic analysis of Ki-67 positive cells in haemopoietic malignancies with peripheral blood disease should be carried out to define more precisely the proportion of normal and neoplastic cells in cycle. (bmj.com)
  • In view of its high sensitivity and specificity in neoplastic tissues, Ki-67 has been widely used as a reliable marker in various studies on oral neoplasms. (thejcdp.com)
  • 9 ] used the preoperative inflammatory, nutritional, and tumor marker index to develop a model that can predict the overall survival of GC patients and obtain good discrimination. (biomedcentral.com)
  • Therefore, it is essential to identify new additional predictive markers, and there is an urgent need for scoring systems that can discriminate postoperative patients by which high-risk patients can be identified, to improve prognostic prediction and provide appropriate treatment. (biomedcentral.com)
  • However, Ki-67 LI can only be obtained by postoperative biopsy pathology, and there is still a lack of effective preoperative predictors. (biomedcentral.com)
  • AIMS: To investigate the numbers, morphology, and lineage assignment of Ki-67 positive cells in peripheral blood from normal subjects. (bmj.com)
  • Additionally, Ki-67 LI is not affected by factors such as age and gender. (biomedcentral.com)
  • In studies of tumor cells, the "Ki-67 labeling index" refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. (thermofisher.com)
  • Forty one per cent of the Ki-67 positive cells were CD3 positive by double immunoenzymatic staining and corresponded to T lymphocytes, and 11.4% were mature B cells expressing kappa or lambda light chains. (bmj.com)
  • Half of the Ki-67 positive lymphocytes could not be accounted for by B or T cells with the markers used. (bmj.com)
  • However the quantitative analysis of p53 positive cells and their localization in oral epithelium is of importance as a marker for oral squamous cell carcinoma. (bvsalud.org)
  • Myelinating OLs originate from parenchymal precursors expressing the neuron-glia antigen 2 (NG2) chondroitin sulphate proteoglycan and the platelet derived growth factor receptor alpha (PDGFRα), commonly referred to as oligodendrocyte progenitor cells (OPCs) 4 . (nature.com)
  • Qualitatively p53 is not a specific marker for malignancy of oral epithelium. (bvsalud.org)
  • Positive immunoreactivity indicated the presence of brown precipitate in the nucleus at the site of the target antigen. (thejcdp.com)
  • The median progression-free survival (PFS) was 97.8 months, and the 1-year and 5-year PFS were 92% and 67%, respectively. (biomedcentral.com)
  • Despite cell losses during the acute phase, upregulation of Ki-67 correlated with recovery of cell populations over time. (cdc.gov)
  • Serologic markers change over typical courses of resolved acute infection and progression to chronic infection ( Figure 1 ) ( 15 ). (cdc.gov)
  • Is Ki-67 of Diagnostic Value in Distinguishing Between Partial and Complete Hydatidiform Moles? (iiarjournals.org)
  • Results: Positive immmuno-staining for Ki67 was identified in 88% of the patients. (journalcra.com)
  • The receiver operating characteristic (ROC) curve was used to estimate the accuracy of each ADC parameter in predicting the Ki-67 level, and finally a multivariate logistic regression model was constructed based on the results of ROC analysis. (biomedcentral.com)
  • Mathematical constraints on FST: multiallelic markers in arbitrarily many populations. (who.int)
  • The cancer diagnostics market is on the verge of explosion, as the researchers approach major technological breakthroughs in tumor diagnosis and therapy, discover new specific antigens, and unlock the mystery of the genetic basis of the disease. (aarkstore.com)