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OligosaccharidesMannosealpha-MannosidaseMannosidasesCarbohydrate SequenceHexosaminidasesMannose-Binding LectinsGlycopeptidesAsparagineGlycosylationGlycoproteinsCarbohydrate ConformationMolecular Sequence DataProtein Processing, Post-TranslationalKineticsCell LineGuanosine Diphosphate MannoseGlycoside HydrolasesMannosephosphatesPolyisoprenyl Phosphate OligosaccharidesReceptor, IGF Type 2PolysaccharidesDolichol Monophosphate MannoseCarbohydratesMannosyl-Glycoprotein Endo-beta-N-AcetylglucosaminidaseOligosaccharides, Branched-ChainFucoseSwainsonineGlucosamineLectinsPolyisoprenyl Phosphate SugarsAcetylglucosamineMannosyltransferasesGalactose1-DeoxynojirimycinMannansMonosaccharidesPeptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine AmidaseDisaccharidesChromatography, High Pressure LiquidSialic AcidsIndolizinesMagnetic Resonance SpectroscopyAcetylglucosaminidaseAcetylgalactosamineTunicamycinChromatography, GelN-Acetylneuraminic AcidDolicholAmino SugarsTrisaccharidesMannosidesDolichol PhosphatesGlycolipidsGlycoconjugatesLectins, C-TypeChromatography, AffinityChromatography, PaperPlant LectinsChromatography, Ion ExchangeElectrophoresis, PaperMannose-6-Phosphate IsomerasePolyisoprenyl Phosphate MonosaccharidesN-AcetylglucosaminyltransferasesHexosephosphatesSugar AlcoholsReceptors, Cell SurfaceGlucosidasesGlycosidesAmino Acid SequenceBorohydridesalpha-GlucosidasesMilk, HumanMolecular WeightMass SpectrometryLysosomesChromatography, Thin LayerSubstrate SpecificityElectrophoresis, Polyacrylamide GelCarbohydrate MetabolismSpectrometry, Mass, Fast Atom BombardmentMucinsalpha-MannosidosisLewis Blood-Group SystemSulfatesPrebioticsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationPeriodic AcidGolgi ApparatusHexosesSulfuric AcidsNeuraminidaseMethylationGlucansbeta-MannosidaseCricetinaeCattleGalactosyltransferasesMethylmannosidesalpha-L-Fucosidase
ResiduesGlucoseGlycoproteinLectinGalactoseFucoseLipid-linked oligosaccharidesGlycoproteins and glycopeptidesGlycansSugarsMembrane glycoproteinsProteinsResiduePrecursorTerminalMoleculesGlcNAcBiantennaryEndo F2GlycopeptidesAsparagineHighStructuresMonosaccharidesProteinReceptorPathwayLysosomalPrimarilyBacteriaCharacteristicTypeYieldSugarEnzymaticOptimalRichHelpsAnalysisIntactUnitsAlpha
Residues8
  • This enzyme catalyses the following chemical reaction Transfers an N-acetyl-D-glucosamine residue from UDP-N-acetyl-D-glucosamine to the 4-position of a mannose linked alpha-(1->6) to the core mannose of high-mannose oligosaccharides produced by Dictyostelium discoideum The activity of the intersecting mannose residue as acceptor is dependent on two other mannose residues attached by alpha-1,3 and alpha-1,6 links. (wikipedia.org)
  • It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. (ludger.com)
  • Peptide- N -Glycosidase F, also known as PNGase F, is an amidase that cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N -linked glycoproteins (1). (neb.com)
  • they contain complex-type oligosaccharides whose nonreducing ends terminate in galactose and sialic acid residues. (rupress.org)
  • However, the major class contains biantennary and more highly branched complex-type oligosaccharides that terminate in N-acetylglucosamine rather than galactose or sialic acid residues. (rupress.org)
  • Glycosylation can be further categorized into N-linked (where the oligosaccharide is conjugated to Asparagine residues) and O-linked glycosylation (where the oligosaccharide is conjugated to Serine, Threonine and possibly Tyrosine residues). (reactome.org)
  • 2)-linked alpha-D-mannose residues in an oligo-mannose oligosaccharide. (ntu.edu.sg)
  • In the case of lysosomal target proteins, for example, mannose residues are phosphorylated. (microbiologiaitalia.it)
Glucose12
  • A number of C-type lectins recognise oligosaccharides rich in mannose and fucose - sugars with similar structures to glucose. (nih.gov)
  • This raises the possibility that high glucose conditions in diabetes affect protein-oligosaccharide interactions via competitive inhibition. (nih.gov)
  • Mannose-binding lectin, soluble DC-SIGN and DC-SIGNR, and surfactant protein D, were tested for carbohydrate binding in the presence of glucose concentrations typical of diabetes, via surface plasmon resonance and affinity chromatography. (nih.gov)
  • High glucose inhibited C-type lectin binding to high-mannose glycoprotein and binding of DC-SIGN to fucosylated ligand (blood group B) was abrogated in high glucose. (nih.gov)
  • The reaction conditions to hydrolyze konjac powder (KP) were investigated by using a combination of microwave radiation heating and HCl as a catalyst to explore the possibility of glucomannan-oligosaccharides, mannose, and glucose extractions from the polysaccharides of KP. (tci-thaijo.org)
  • Selective reaction conditions to produce glucomannan-oligosaccharides, mannose, and glucose were determined. (tci-thaijo.org)
  • The highest yield for both mannose and glucose production, at 35.8% and 30.2%, respectively, was achieved under the reaction conditions of 110°C, 15 min, 1.2 M HCl and 10:1 (mL/g) ratio of reaction volume to KP mass. (tci-thaijo.org)
  • In this study, we analyze the size distribution and structure of N-linked oligosaccharides attached to the recombinant glycoprotein glucose oxidase (GOD) and the cell wall mannoproteins obtained from H. polymorpha. (unboundmedicine.com)
  • When examined from the viewpoint of the bound sugar, the structural information accumulated so far on lectins and other proteins that are specific to galactose and glucose (or mannose), provides suggestive evidence for distinct ligand-dependent distribution of hydrogen-bond partners in the combining site. (embl.de)
  • These are typically short chains of fructo-oligosaccharides (FOS), which are a mix of fructose and glucose, mannose oligo-saccharides (MOS) or galacto-oligosaccharides (GOS). (omegafields.com)
  • Both the polysaccharides in RRP-S and RRP-W were composed of mannose, galacturonic acid, glucose, galactose and arabinose, but with different molar ratios (2.57:0.05:1.00:0.87:0.10 vs 2.07:0.07:1.00:1.05:0.17). (edu.hk)
  • Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. (biotrend.com)
Glycoprotein2
  • The structures of the asparagine-linked oligosaccharides of several variant forms of the vesicular stomatitis virus glycoprotein transiently expressed from cloned cDNAs have been determined. (rupress.org)
  • Another mutant, delta 1554, codes for a glycoprotein that contains oligosaccharides of primarily two classes. (rupress.org)
Lectin2
Galactose2
  • Inspired by the multivalency, heterogeneity and nature of carbohydrate ligand-mediated interactions, we constructed a combinatorial library of mannose and galactose homo-and hetero-glycodendrons to study CPIs. (iucc.ac.il)
  • In the absence of calcium, rToVIP36-LTLD proteins bound to the bacteria by binding to lipopolysaccharides, peptidoglycans, d-mannose, and d-galactose and inhibited the growth of S. aureus and V. harveyi. (bvsalud.org)
Fucose4
Lipid-linked oligosaccharides1
Glycoproteins and glycopeptides2
  • Endo F2 is a highly specific recombinant endoglycosidase which cleaves within the chitobiose core of asparagine-linked complex biantennary and high mannose oligosaccharides from glycoproteins and glycopeptides. (neb.com)
  • PNGase F is suitable for release of all types (high-mannose, hybrid and complex) N-linked glycans from glycoproteins and glycopeptides. (ludger.com)
Glycans7
  • Endo F2 cleaves biantennary glycans at a rate approximately 20 times greater than high mannose glycans. (neb.com)
  • Griffithsin's antiviral activity stems from its ability to bind terminal mannoses present in high-mannose oligosaccharides and crosslink these glycans on the surface of the viral envelope glycoproteins. (mdpi.com)
  • Endo F1 cleaves high mannose and some hybrid type N-glycans from peptides and proteins. (ludger.com)
  • It also will cleave high mannose glycans but at a 40x reduced rate. (ludger.com)
  • Analyses using anti-alpha 1,3-mannose antibody and exoglycosidases specific for alpha 1,2- or alpha 1,6-mannose linkages revealed that the mannose outer chains of N-glycans on the rGOD have very short alpha 1,6 extensions and are mainly elongated in alpha 1,2-linkages without a terminal alpha 1,3-linked mannose addition. (unboundmedicine.com)
  • The N-glycans released from the H. polymorpha mannoproteins were shown to contain mostly mannose in their outer chains, which displayed almost identical size distribution and structure to those of H. polymorpha-derived rGOD. (unboundmedicine.com)
  • These results strongly indicate that the outer chain processing of N-glycans by H. polymorpha significantly differs from that by Saccharomyces cerevisiae, thus generating much shorter mannose outer chains devoid of terminal alpha 1,3-linked mannoses. (unboundmedicine.com)
Sugars1
Membrane glycoproteins1
Proteins4
Residue3
  • Sequential mannose trimming of N -glycan, from M9 to M8B and then to oligosaccharides exposing the α1,6-linked mannosyl residue (M7A, M6, and M5), facilitates endoplasmic reticulum-associated degradation of misfolded glycoproteins (gpERAD). (elifesciences.org)
  • PNGase F digestion deaminates the asparagine residue to aspartic acid, and leaves the oligosaccharide intact for further analysis. (neb.com)
  • The addition of a precursor 13-residue oligosaccharide moiety GlcNAc2Man8Gluc3 to the nascent polypeptide chain occurs in the endoplasmic reticulum, following which the assembly folds and acquires its secondary, tertiary, and quaternary structure. (chromatographyonline.com)
Precursor1
  • The GPIIb precursor is then processed with conversion of high-mannose to complex type carbohydrates yielding the mature subunits GPIIb alpha (Mr 116,000) and GPIIb beta (Mr 25,000). (rupress.org)
Terminal1
  • α(1-6) Core Mannosidase cleaves unbranched non-reducing terminal mannose, α(1-6) linked to the β-linked core mannose of the conserved mannosylchitobiose core of N-linked oligosaccharides. (ludger.com)
Molecules2
GlcNAc1
  • High-mannose-oligosaccharide beta-1,4-N-acetylglucosaminyltransferase (EC 2.4.1.197), uridine diphosphoacetylglucosamine-oligosaccharide acetylglucosaminyltransferase, acetylglucosamine-oligosaccharide acetylglucosaminyltransferase, UDP-GlcNAc:oligosaccharide beta-N-acetylglucosaminyltransferase, UDP-N-acetyl-D-glucosamine:high-mannose-oligosaccharide beta-1,4-N-acetylglucosaminyltransferase) is an enzyme with systematic name UDP-N-acetyl-D-glucosamine:high-mannose-oligosaccharide 4-beta-N-acetylglucosaminyltransferase. (wikipedia.org)
Biantennary1
Endo F21
Glycopeptides1
  • We have identified a novel class of peptides bound to MHC class II: mannose 6- phosphate (Man-6-P) containing glycopeptides from lysosomal enzymes. (ox.ac.uk)
Asparagine3
  • Endo-beta-N-acetylglucosaminidase H hydrolyzes the beta-(1-4)-glycosidic link of the N,N'-diacetylchitobiose core of high-mannose and hybrid asparagine-linked oligosaccharides. (rcsb.org)
  • Endo H cleaves Asparagine-linked hybrid or high mannose oligosaccharides, but not complex oligosaccharides. (ludger.com)
  • Processing of the asparagine-linked oligosaccharides of secreted and intracellular forms of the vesicular stomatitis virus G protein: in vivo evidence of Golgi apparatus compartmentalization. (rupress.org)
High4
Structures1
  • Microarray analysis of these glycodendrons with a wide range of biologically important plant and animal lectins revealed that oligosaccharide structures and heterogeneity interact with each other to alter binding preferences. (iucc.ac.il)
Monosaccharides1
  • The combination of microwave radiation (MCR) heating and HCl effectively hydrolyzed konjac powder selectively into glucomannan-oligosaccharides and/or monosaccharides in a relatively short reaction time. (tci-thaijo.org)
Protein2
  • Higher selectivity of binding is achieved by extending binding sites through additional direct and water-mediated contacts between oligosaccharides and the protein surface. (embl.de)
  • In contrast, forms of the G protein that remain intracellular possess oligosaccharides at intermediate stages in the processing pathway. (rupress.org)
Receptor4
  • The mannose 6-phosphate/insulin-like growth factor II receptor (M6P/IGF2R) is a multifunctional tumour suppressor which binds a variety of factors that impinge on the proliferation, migration and invasiveness of cancer cells. (boku.ac.at)
  • These species were identified in the process of characterizing mannose 6-phosphate/insulin-like growth factor II (M-6-P/IGF-II) receptor binding to the surface of B lymphoblasts. (ox.ac.uk)
  • Surface iodination and Man-6-P/IGF-II receptor affinity chromatography implicated MHC class II as a carrier of Man-6-P-modified oligosaccharides. (ox.ac.uk)
  • The mouse Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 175 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). (rndsystems.com)
Pathway1
  • This study demonstrates the details of mannose trimming in the ER by EDEM1 and EDEM3, filling an important gap in how mannose trimming creates an ERAD signal and shunts glycoproteins to the ERAD pathway. (elifesciences.org)
Lysosomal1
  • A mannose 6-phosphate-containing N-linked glycopeptide derived from lysosomal acid lipase is bound to MHC class II in B lymphoblastoid cell lines. (ox.ac.uk)
Primarily1
Bacteria1
Characteristic1
Type1
Yield1
  • The highest yield for glucomannan-oligosaccharides production, at 19.2%, was achieved under the reaction conditions of 110°C, 15 min, 2 M HCl and 10:1 (mL/g) ratio of reaction volume to KP mass. (tci-thaijo.org)
Sugar1
Enzymatic1
Optimal1
  • Oligosaccharides and Hydrolyzed Beta Glucan respond to dehydration signals, helping skin to maintain optimal hydration levels. (dermalogica.com)
Rich1
  • Hydrolyzed Wheat Flower , rich in Arabinoxylo- Oligosaccharides, activates as needed to brighten and help reduce the appearance of dark spots. (dermalogica.com)
Helps1
  • Mannose Oligosaccharide market analysis helps to understand key industry segments, and their global, regional, and country-level insights. (cognitivemarketresearch.com)
Analysis2
Intact1
  • In contrast, PNGase F removes the oligosaccharide intact. (ludger.com)
Units1
  • in the second case instead, three units of mannose are eliminated, but at the same time other trisaccharide units are added. (microbiologiaitalia.it)
Alpha1