Mammalian cells cell fractionation. Medical search. Frequent questions

Mast cells are broadly distributed throughout mammalian tissues and play an important role as regulators of allergic inflammation in various allergy-related disorders, such as asthma, atopic dermatitis, eczema and sinusitis. (spandidos-publications.com)
Also known as mucopolysaccharides because of their viscous properties, GAGs are a family of natural anionic polycarbohydrates found in mammalian tissues, where they play a crucial role in different biological processes. (dovepress.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)

However, a subcellular fractionation assay revealed increased mitochondrial NR4A3 in MOC31PE treated cells, suggesting a role for this protein in MOC31PE-induced apoptotic cell death. (biomedcentral.com)

In contrast to a differential centrifugation method from the literature, analysis of cell-compartment marker proteins shows that the Qproteome Mitochondria Isolation Kit provides mitochondrial fractions virtually free of cross-contamination (see figure "Mitochondrial fractions virtually free of cross-contamination" ). (qiagen.com)
We initially used maps statically to generate a database with localization and absolute copy number information for over 8700 proteins from HeLa cells, approaching comprehensive coverage. (elifesciences.org)
Proteins are the tools and machines of the cell, and each organelle has its own set of proteins that it requires to work correctly. (elifesciences.org)
Each cell contains ten or more organelles, and several thousand different types of proteins. (elifesciences.org)
The location of many proteins in a cell is unclear or simply not known. (elifesciences.org)
set out to develop a method that reveals the locations of all the proteins in a cell at any given time. (elifesciences.org)
The resulting technique maps the location of most of the proteins in a human cancer cell line and, in addition, determines how many copies of each protein there are. (elifesciences.org)
I am using fluorescent protein tagged virus proteins and a range of markers that label different cellular compartments to look at what happens during virus infection in live cells. (brookes.ac.uk)
Left to right: single cell volume histogram, western blots of actin, profilin1 (left: cell titration, right: standard curve of recombinant proteins), values are mean (N = 3) and SD, Materials and methods. (elifesciences.org)
Left to right: name of cell type and origin, histograms of the single cell volume from fluorescence eXclusion measurements, quantitative western blot analysis of cellular actin, profilin1 and profilin-2 amount (left: titration of cell number, right: standard curve of recombinant proteins), calculated mean protein concentration [μM] per cell with experimental error (SD, N = 3 independent experiments, see Materials and methods). (elifesciences.org)
In eukaryotic cells, proteins synthesised in the endoplasmic reticulum (ER) are appropriately folded and assembled before leaving the ER. (nature.com)
Progress in designing complex genetic switches and circuits, expanding the genetic code, modifying cellular organization, producing proteins using cell-free systems, and developing biodesign automation tools is also covered. (cshlpress.com)
As most SUMO target proteins are modified at very low steady-state levels in vivo , we ectopically expressed TDP1 and SUMO1 in mammalian cells and examined the possibility of covalent TDP1-SUMO1 conjugations. (nature.com)
The AP-1B clathrin adaptor complex plays a key role in the recognition and intracellular transport of many membrane proteins destined for the basolateral surface of epithelial cells. (rupress.org)
To a large extent, plasma membrane proteins are delivered to and maintained at the appropriate cell surface domain by distinct targeting signals that control polarized transport on the secretory and endocytic pathways ( Mellman, 1996 ). (rupress.org)
A. Suzuki, M. Tsuda and Y. Saga, "Functional Redundancy among Nanos Proteins and a Distinct Role of Nanos2 during Male Germ Cell Development," Development, Vol. 134, No. 1, 2007, pp. 77-83. (scirp.org)
Important modulators of NF-κB activation are the inhibitor proteins IκBα and IκBβ (referred to herein as IκB), which associate with (and thereby inactivate) NF-κB in the cytoplasm of nonstimulated cells. (justia.com)
Cell 75:495, 1993), and a series of unique E6-AP homologous proteins have been identified (Huibregtse et al. (justia.com)
Thousands of mammalian intracellular proteins are dynamically modified by O-linked β-N-acetylglucosamine ( O -GlcNAc). (mcw.edu)
Using a combination of tissue fractionation, immunoblotting, and galactosyltransferase labeling, as well as hearts from wild-type and O -GlcNAc transferase transgenic mice, we demonstrate that contractile proteins in the heart are differentially detected by two commercially available antibodies (CTD110.6 and RL2). (mcw.edu)
Rapidly growing tumor cells must synthesize proteins at a high rate and therefore depend on an efficient folding and quality control system for nascent secretory proteins in the endoplasmic reticulum (ER). (oncotarget.com)
In an urgent need for new drug targets the endoplasmic reticulum (ER) has become a promising candidate since rapidly growing tumor cells upregulate their protein synthesis and therefore depend on efficient folding of nascent secretory proteins in the ER. (oncotarget.com)
However, uropod-localized receptors and ezrin/radixin/moesin proteins still cluster in nocodazole-treated cells, indicating that MTs are required specifically for uropod stability. (exeter.ac.uk)
In attempts to identify cell-surface receptors for Wnt proteins, we have developed several bioassays for Wnt genes and learned to make cell-free Wnt protein in a complex with the surface antigen of hepatitis B virus. (nih.gov)
Retroviral Receptors Entry of retroviruses into cells depends upon host-encoded transmembrane proteins that serve as receptors for viral envelope glycoproteins. (nih.gov)
The mammalian genome encodes hundreds of tail anchored (TA) membrane proteins with essential roles in diverse processes such as vesicular trafficking, apoptosis, signal transduction, and lipid biosynthesis ( 1 ). (sciencemag.org)

These findings suggest that UVA activated the genotoxicity and cytotoxicity of DPE in mammalian cells and that 1O 2 played an important role in these processes. (nih.gov)
We found that CD8+ T-cell tumor infiltration corresponded to response to treatment, and that anti-PD-L1 gene signature response indicated an increase in antigen processing and presentation, cytokine-cytokine receptor interaction, and natural killer cell-mediated cytotoxicity. (bvsalud.org)
This result is in agreement with the report that showed that macelignan had weak cytotoxicity against the HepG2 and MDA cells (21) . (researchgate.net)
Furthermore, macelignan showed a narrow-ranged spectrum against various fungal strains without any mammalian cell cytotoxicity. (researchgate.net)

A complete RNA polymerase II transcription system has been derived by the fractionation of yeast and mammalian cell extracts. (nih.gov)
Several major species of E2 enzymes have been characterized in mammalian cells, plants, and yeast. (justia.com)
the determinants of frameshifting 261 HAROLD E. VARMUS efficiency can now be studied with genetic, structural, and biochemical methods in yeast and mammalian systems. (nih.gov)

The cAMP and intracellular calcium levels were measured to clarify the mechanisms by which AEDK inhibits the release of histamine from mast cells. (spandidos-publications.com)
AEDK inhibited the release of histamine and β-hexosaminidase from mast cells by modulating cAMP and intracellular calcium levels. (spandidos-publications.com)
Our attempts to understand how retroviruses multiply within infected cells and how retroviruses cause cancers have led to work on a variety of topics, including cell surface receptors, DNA recombination, translational control, inter- and intracellular signaling, hematopoiesis, embryonic development, and mammary carcinogenesis. (nih.gov)

The event of chromatolysis is also characterized by a prominent migration of the nucleus towards the periphery of the cell and an increase in the size of the nucleolus, nucleus, and cell body. (wikipedia.org)
Peripheral chromatolysis is essentially the reverse of central chromatolysis, in which the disintegration of Nissl bodies is initiated at the periphery of the neuron and extends inwards towards the nucleus of the cell. (wikipedia.org)
Peripheral chromatolysis has been observed to occur in lithium-induced chromatolysis and it could be useful in investigating and countering the hypothesis that waves of enzymatic activity always progress from the perinuclear area, or the area situated around the nucleus, to the peripheral of the cell. (wikipedia.org)
p53R2 is induced by p53 after DNA damage and was proposed to supply deoxynucleotides for DNA repair after translocating from the cytosol to the cell nucleus. (nih.gov)
In this talk I will discuss our work on the physicochemical assembly and properties of the nucleolus, the largest structure within the cell nucleus. (nih.gov)
I am interested in how baculovirus travels within cells and in particular, the role of the cytoskeleton in transport of virus from the nucleus to cell surface. (brookes.ac.uk)

One of the first published papers to report the use of iodixanol gradients for the analysis of endoplasmic reticulum (ER), Golgi and plasma membrane (PM) from COS-7 cells was by Yang et al [1] in 1997. (axis-shield-density-gradient-media.com)
2. Visualization of Endoplasmic Reticulum-Associated mRNA in Mammalian Cells. (nih.gov)
6. Analysis of mRNA partitioning between the cytosol and endoplasmic reticulum compartments of mammalian cells. (nih.gov)
14. Hierarchical regulation of mRNA partitioning between the cytoplasm and the endoplasmic reticulum of mammalian cells. (nih.gov)
18. Analyzing mRNA localization to the endoplasmic reticulum via cell fractionation. (nih.gov)
19. Divergent regulation of protein synthesis in the cytosol and endoplasmic reticulum compartments of mammalian cells. (nih.gov)
Dynamic release from calmodulin allowed sampling of the endoplasmic reticulum (ER), where the conserved E R m embrane protein c omplex (EMC) was shown to be essential for efficient insertion in vitro and in cells. (sciencemag.org)

We aimed to investigate the synergistic effects of DPE and UVA at low-dose exposures in human-hamster hybrid (A L ) cells and their underlying mechanisms. (nih.gov)
Cell-cell interactions, stromal cells, drug resistance, in vitro evaluation of anticancer agents, mechanisms of invasion and metastasis. (iiar-anticancer.org)
This study aimed to investigate the protective effects of the aqueous extract of Diospyros kaki (AEDK) on mast cell-mediated allergic inflammation and to determine its possible mechanisms of action by using in vitro and in vivo mast cell-based models. (spandidos-publications.com)
Furthermore, the biochemical pathways and mechanisms that determine isotopic fractionation during biosilicification remain poorly understood. (copernicus.org)
It involves the breakage of the cell membrane by different mechanisms. (uberant.com)
We are interested in signal transduction mechanisms that operate in mammalian cells. (uth.edu)
Research themes in the laboratory are diverse, and include: investigation of the cell biology of Ras protein trafficking between the ER and plasma membrane, analysis of the molecular mechanisms that govern Ras interactions with the plasma membrane, proteomic and functional characterization of plasma membrane nanodomains, caveolae and lipid rafts, and mathematical modeling of Ras signaling networks. (uth.edu)
My recent research greatly focuses on the study of molecular mechanisms underlining cell movement. (exeter.ac.uk)
My research focuses on understanding the molecular mechanisms underlining cell migration, which are fundamental to a variety of physiological and pathological processes, including embryonic development, tissue repair, immune responses, and cancer cell metastasis. (exeter.ac.uk)

Based on functional enrichment analyses, up-regulated polyploidy genes appeared to implicate several functions, which primarily include cell/nuclear division, ATP binding, metabolic process, and mitochondrial activity, whereas that of down-regulated genes primarily included apoptosis and immune processes. (plos.org)
Consistently, studies revealed a marked induction of mitochondrial mass and ATP production in polyploid cells. (plos.org)
The inhibition of mitochondrial activity by various pharmacological inhibitors, as well as by gene-specific targeting using siRNA-mediated technology showed a dramatic attenuation of polyploidy and bi-nucleation development during in vitro stromal cell decidualization, suggesting mitochondria play a major role in positive regulation of decidual cell polyploidization. (plos.org)
The end result is increased sensitivity of T cells and thymocytes to spontaneous mitochondrial damage, leading to T-cell depletion due to apoptosis. (medscape.com)

A tutorial in my laboratory includes an introduction to a variety of advanced imaging techniques such as confocal microscopy, FRET and FLIM imaging, TIRF microscopy and electron microscopy, as well as cellular and biochemical assays of signaling pathways, coupled with a firm grounding in basic cell and molecular biological methodologies that include cell culture and transfection, cell fractionation, immunoblotting, immunoprecipitation and protein purification. (uth.edu)
Transfection of CHO and PC12 cells with a cDNA encoding the rat major vault protein containing a vesicular stomatitis virus glycoprotein epitope tag demonstrates that the recombinant protein is sorted into vault particles and targeted like endogenous MVPs. (silverchair.com)

Exosomes contain cargo material from endosomes, cytosol, plasma membrane and microRNA molecules, they are released by a number of non-cancer and cancer cells into both the extracellular microenvironment and body fluids such as blood plasma. (brookes.ac.uk)
Extracellular vesicles (EVs) are cell-derived membranous nanoparticles present in most biologic fluids. (cdc.gov)

From observing the regression of ovarian follicles in mammals, it was argued that a necessary cellular process existed to counterbalance the proliferation of cells by mitosis. (wikipedia.org)
Upon antigen stimulation they rapidly upregulate glycolysis and oxygen to produce high levels of ATP and nucleotides required for cell proliferation and effector functions. (nih.gov)
Investigation of the effects of MOC31PE treatment on protein synthesis, cell viability, proliferation and gene expression of the ovarian cancer cell lines B76 and HOC7. (biomedcentral.com)
Nevertheless, we observed a strong reduction of proliferation in response to ERp57 knockdown in both cell lines regardless of the p53 status. (oncotarget.com)

Its objective is to stimulate research and technology development to promote the early-stage preclinical validation of therapeutic leads (that need not be finalized therapeutics, henceforth called "therapeutic leads") such as small molecules or non-viral biologics (e.g. antibodies, cell-based therapies, engineered tissue constructs, probiotic or commensal microbes) that are not currently a focus within the biotechnology and pharmaceutical industries. (nih.gov)
T-cell receptor- and CD28-induced Vav1 activity is required for the accumulation of primed T cells into antigenic tissue. (exeter.ac.uk)
Localization of primed T cells to antigenic tissue is essential for the development of effective immunity. (exeter.ac.uk)

The Global Cell Fractionation market is broadly categorized on the basis of Product into Consumables, Reagents, Beads, Disposables and Instruments. (uberant.com)

Our results highlight that there is a taxonomic component to silicon isotope fractionation during biosilicification, possibly via a shared or related biochemical transport pathway. (copernicus.org)
Cell fractionation assists the scientists to study the various parts of cells in separation to identify their biochemical function and structure. (uberant.com)

Instead of a direct interaction with p53, depletion of ERp57 induced cell death via a selective activation of the PERK branch of the Unfolded Protein Response (UPR). (oncotarget.com)
In a further description of the mechanism of T-cell depletion in purine nucleoside phosphorylase deficiency, Arpaia et al reported increased in vivo apoptosis of T cells and increased in vitro sensitivity to gamma irradiation in a murine model. (medscape.com)

The hallmark of eukaryotic cells is their compartmentalization into distinct membrane-bound organelles. (elifesciences.org)

B ) Purification of mammalian cytoplasmic actin from two sources. (elifesciences.org)
C ) Barbed end polymerization rate of cytoplasmic native mammalian (β,γ)-actin as a function of the actin monomer concentration. (elifesciences.org)

The consistent features of chromatolysis included the condensation of the cytoplasm and chromatin, cell shrinkage, formation of "chromatin balls," intact normal organelles, and fragmentation of cells observed by the budding of fragments enclosed in the cell membrane. (wikipedia.org)
Like other members of the Rel family of transcriptional activators, NF-κB is sequestered in an inactive form in the cytoplasm of most cell types. (justia.com)

However, studies have also shown that it is non-essential for virus replication, at least in cell culture. (brookes.ac.uk)

A collection of offprints of historic experimental papers in 20th century biological sciences: biochemistry, cell biology, genetics, molecular biology. (goldwasserbooks.com)
Dynamic Organellar Maps enable the proteome-wide analysis of physiological protein movements, without requiring any reagents specific to the investigated process, and will thus be widely applicable in cell biology. (elifesciences.org)
I gained my PhD in plant cell biology from Oxford Brookes University in 2004, which was provided great training in different imaging techniques which I continue to use today. (brookes.ac.uk)
Address correspondence to Ira Mellman, Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar St., PO Box 208002, New Haven, CT 06520-8002. (rupress.org)
These research areas are among the most exciting and controversial in contemporary cell biology. (uth.edu)
genomic and chemical discovery programs therefore operate in parallel with all of our basic cell biology projects. (uth.edu)
My scientific expertise includes biochemistry, molecular biology, pharmacology, immunology and mouse genetics in combination with cell biology techniques. (exeter.ac.uk)

Fibroblasts against cancer cells in vitro. (iiar-anticancer.org)
Proliferating fibroblasts and HeLa cells co-cultured in vitro reciprocally influence growth patterns, protein expression, chromatin features and cell survival. (iiar-anticancer.org)
Interactions between human fibroblastsand HeLa cells in vitro. (iiar-anticancer.org)
Interactions between human lymphoblastoid cells and human fibroblast feeder layers in vitro. (iiar-anticancer.org)

The interior of every cell is highly organised, and contains many compartments, called organelles, that are dedicated to specific roles. (elifesciences.org)

16. Translation dynamics of single mRNAs in live cells and neurons. (nih.gov)

Quantification of profilin-actin levels and purification of mammalian profilin-actin. (elifesciences.org)
His experience includes CMC development (cell culture, purification process, formulation, and analytical methods) and interfacing with regulatory and clinical development for multiple marketing and clinical stage biologics. (chi-peptalk.com)

In the present study, to better define the aspects of decidual cell polyploidy, we isolated pure polyploid and non-polyploid decidual cell populations from the in vivo decidual bed. (plos.org)
The zebrafish embryo is an ideal model system for studying PGC migration due to 1) the transparency of the embryo, allowing in vivo analyses of cell migration and development, 2) the availability of various mutant stocks and the morpholino antisense oligo nucleotide gene knockdown system, and 3) the availability of PGC marker genes, for which localisation and functions are well characterised. (exeter.ac.uk)

Subcellular localization critically influences protein function, and cells control protein localization to regulate biological processes. (elifesciences.org)
S. Yokota and Y. Onohara, "Expression and Localization of NANOS1 in Spermatogenic Cells during Spermatogenesis in Rat," CellBio , Vol. 2 No. 1, 2013, pp. 1-10. (scirp.org)

In the receptive uterus on day 4 of pregnancy (day 1 = vaginal plug), the onset of embryo implantation elicits the prerequisite transformation of stromal cells into decidual cells (decidualization), which is a gateway to pregnancy establishment. (plos.org)

Therefore, the inhibition of these inflammatory cytokines produced by mast cells is one of the most important targets for the reduction of allergic inflammatory symptoms. (spandidos-publications.com)
These activities include prevention or restoration of the integrity and permeability of EC, counteraction versus chemical, toxic or metabolic EC injury, regulation of EC-blood cell interactions, inhibition of microvascular inflammatory and proliferative changes, and other similar effects, thus allowing oral SDX to be considered as an endothelial-protecting agent. (dovepress.com)
By combining MOC31PE and the immune suppressor cyclosporin A (CsA) the MOC31PE effect on protein synthesis inhibition and cell viability increased tenfold. (biomedcentral.com)
Nocodazole stimulates RhoA activity, and inhibition of the RhoA target ROCK allows nocodazole-treated cells to re-establish lamellipodia and uropods and persistent migratory polarity. (exeter.ac.uk)

Cytogenetical changes during early stages of developmentof methotrexate resistance in HeLa cells. (iiar-anticancer.org)
Combining spatial and abundance information yielded an unprecedented quantitative view of HeLa cell anatomy and organellar composition, at the protein level. (elifesciences.org)

Y. Wang, R. M. Zayas T. Guo and P. A. Newmark, "Nanos Function Is Essential for Development and Regeneration of Planarian Germ Cells," Proceedings of National Academy of Sciences of the United States of America, Vol. 104, No. 14, 2007, pp. 5901-5906. (scirp.org)
Y. Hayashi, M. Hayashi and S. Kobayashi, "Nanos Suppresses Somatic Cell Fate in Drosophila Germ Line," Proceedings of National Academy of Sciences of the United States of America, Vol. 101, No. 28, 2004, pp. 10338-10342. (scirp.org)

Biol Cell 59(1):69-77, 1987. (iiar-anticancer.org)
Biol Cell 50(1):9-16, 1984. (iiar-anticancer.org)

The cells showed variable stages of pyknotic chromatin. (wikipedia.org)
Other stages included cell fractionation into smaller bodies. (wikipedia.org)
The research on brite cells is still in early stages, but we now know enough about how they are formed and the metabolic advantage they are capable of providing that we can target them as a viable and important angle in weight management and metabolic optimization. (anabolicminds.com)

In addition to the studying orginary cell migration, I also plan to investigate the effects of environmental toxins on PGC migration, which have been shown to alter germ cells and the gonads development, leading to the reproductive defects. (exeter.ac.uk)

Nonrandom association of acrocentric chromosomes in human epithelial cells. (iiar-anticancer.org)
The MOC31PE immunotoxin binds to the cell surface antigen EpCAM, which is expressed by the majority of epithelial cancers including ovarian carcinomas, and we studied the cytotoxic effects of MOC31PE in ovarian cancer cells. (biomedcentral.com)
Recently, it has been suggested that EpCAM is a cancer stem cell marker and may be expressed by cells undergoing epithelial to mesenchymal transition (EMT), lacking other epithelial markers[ 4 ]. (biomedcentral.com)

The process he described now fits with the relatively new term, apoptosis, to describe cell death. (wikipedia.org)
These budding fragments were termed "apoptotic bodies," thus coining the name "apoptosis" to describe this form of cell death. (wikipedia.org)
We exposed exponentially growing A L cells to DPE and/or UVA radiation with or without reactive oxygen species (ROS) quenchers and then assayed the cells for survival, mutation induction, apoptosis, and micronucleus generation. (nih.gov)
In contrast, apoptosis was reduced in MDA-MB-231 breast cancer cells harboring mutant p53. (oncotarget.com)

the compounds exerted no significant effect against the tumor cells. (researchgate.net)
One of the major challenges of current cancer treatment is the resistance of tumor cells to ionizing radiation (IR) and chemotherapeutical substances. (oncotarget.com)

Over the course of infection in cultured cells, the cage remodels to form a large polarised P10 mass and we suggest that these changes are critical for nuclear lysis to release occlusion bodies. (brookes.ac.uk)
Mast cell activation is initiated by the phosphorylation of tyrosine kinase which leads to the activation of protein kinase C, nuclear factor (NF)-κB and the expression of pro-inflammatory cytokines ( 4 , 8 ). (spandidos-publications.com)
The S. cerevisiae SPB is embedded in the nuclear envelope during the entire cell cycle. (sdbonline.org)

K. Subramaniam and G. Seydoux, "Nos-1 and Nos-2, Two Genes Related to Drosophila Nanos, Regulate Primordial Germ Cell Development and Survival in Caenorhabditis elegans," Development, Vol. 126, No. 21, 1999, pp. 4861-4871. (scirp.org)
G. Deshpande, G. Calhoun, J. L. Yanowitzand and P. D. Schedl, "Novel Functions of nanos in Down Regulating Mitosis and Transcription during the Development of the Drosophila Germline," Cell, Vol. 99, No. 3, 1999, pp. 271-181. (scirp.org)
J Cell Biol (1999) 144 (6): 1163-1172. (silverchair.com)

perhaps the most important example is the lymphocyte cell-surface antigen CD4, a simple transmembrane glycoprotein that is required for attachment of HIV to target cells. (nih.gov)

APC) capture, process and present antigen to effector T and B cells (adaptive immunity). (nih.gov)
Immediate-type hypersensitivity is mediated by histamine in response to the antigen cross-linking of immunoglobulin E (IgE) bound to FcɛRI on mast cells ( 3 ). (spandidos-publications.com)
ITs consist of an antibody, that with high affinity binds to the target antigen on the cancer cell surface, and a covalently bound toxin. (biomedcentral.com)

For early treatment assessment, we analyzed gene expression profiles, T-cell infiltration, and T-cell receptor (TCR) signatures in regressing tumors compared with tumors exhibiting no response to anti-PD-L1 treatment. (bvsalud.org)
Mammalian cells have 2 additional ER stress sensors: the membrane-bound transcription factor ATF6 and the receptor-type protein kinase PERK. (nature.com)

Chromatolysis is still used as a term to distinguish the particular apoptotic process in the neuronal cells, where Nissl substance disintegrates. (wikipedia.org)
The knockdown of ERp57 significantly enhanced the apoptotic response to anticancer treatment in HCT116 colon cancer cells via a p53-dependent mechanism. (oncotarget.com)

In cellular neuroscience, chromatolysis is the dissolution of the Nissl bodies in the cell body of a neuron. (wikipedia.org)
Cell fractionation, also called as Cell Lysis, is the process of separating cellular parts and retaining the independent functions of each part. (uberant.com)

Exosomes extracted from conditioned media of irradiated and bystander progeny were added to unirradiated cells. (brookes.ac.uk)

Teaming Up for Trouble: Cancer Cells, Transforming Growth Factor-β1 Signaling and the Epigenetic Corruption of Stromal Naïve Fibroblasts. (iiar-anticancer.org)

Establishment and characterization of a B-cell line from a patient with acute lymphoblastic leukemia. (iiar-anticancer.org)

One survival mechanism of cells under stress conditions is the activation of the Unfolded Protein Response (UPR) through one of its three ER-stress sensors IRE1, PERK and ATF6. (oncotarget.com)

MOC31PE treatment for 24 h caused a dose-dependent reduction of protein synthesis with ID 50 values of less than 10 ng/ml, followed by reduced cell viability. (biomedcentral.com)

The process of Nissl dissolution is less apparent toward periphery of the cell body of the neuron, where normal-looking Nissl bodies may be present. (wikipedia.org)

The treatment of intact cell nuclei may also result in the formation of DNA-DNA cross-links. (nih.gov)

Microtubules regulate migratory polarity through Rho/ROCK signaling in T cells. (exeter.ac.uk)
Microtubules (MTs) are required for persistent migration and chemotaxis, but how they affect cell polarity is not known. (exeter.ac.uk)

The ability to monitor changes in organellar composition would provide a powerful tool to investigate cell biological processes at the systems level. (elifesciences.org)

C ) Profilin-actin measurements in HT1080 cells. (elifesciences.org)
Here, we present the first measurements of the silicon isotopic fractionation during biosilicification by loricate choanoflagellates, a group of protists closely related to animals. (copernicus.org)

Around the same time of Flemming's research, chromatolysis was also studied in the lactating mammary glands and in breast cancer cells. (wikipedia.org)
Notably, MOC31PE also induces cell death in chemotherapy-resistant cancer cells[ 7 ] and may hence be used in patients with recurrent disease lacking other therapeutic options. (biomedcentral.com)

Double minutes in fibroblast-like cells isolated from human tumors. (iiar-anticancer.org)
We generated an orthotopic brain tumor model derived from a GEM model with Rb, Kras, and p53 aberrations (TRP), which develops GBM tumors displaying linear foci of necrosis by neoplastic cells, and dense vascularization analogous to human GBM. (bvsalud.org)
Cells derived from GEM GBM tumors are injected intracranially into wild-type, strain-matched recipient mice and reproduce grade IV tumors, therefore bypassing the long tumor latency period in GEM mice and allowing for the creation of large and reproducible cohorts for preclinical studies. (bvsalud.org)
Molecular subtypes of small cell lung cancer (SCLC) defined by the expression of key transcription regulators have recently been proposed in cell lines and limited number of primary tumors. (bvsalud.org)
Our data demonstrates that upon infiltrating the hypoxic, hypoglycemic and acidic microenvironment present in tumors or in a site of chronic inflammation, myeloid cells undergo a dramatic metabolic reprogramming from the use of glucose to fatty acid oxidation. (nih.gov)

Demonstration of physical-chemical changes and analyses of rapid swelling in nerve fibers, cells and synapses associated with excitation processes. (nih.gov)
Collectively, analyses of unique polyploidy markers and molecular signaling networks may be useful to further characterize functional aspects of decidual cell polyploidy at the site of implantation. (plos.org)

Two distinct pathways exist towards increasing brite cells in adult humans, (1) triggering precursor cells into becoming brite cells, and (2) turning mature WAT cells into brite cells. (anabolicminds.com)
We want to target these pathways directly so that we can increase the ratio of brite cells to WAT cells, allowing your body to constantly burn more calories. (anabolicminds.com)
In a gene expression array monitoring the expression of 84 key genes in cancer pathways, 13 of the genes were differentially expressed by MOC31PE treatment in comparison to untreated cells. (biomedcentral.com)

Let's dive into some of the known methods of brite cell creation and activation. (anabolicminds.com)

We propose that regulation of MT stability and RhoA/ROCK activity is a mechanism to alter T-cell migratory behavior from lamellipodium-based persistent migration to bleb-based migration with frequent turning. (exeter.ac.uk)

Cell 86:453, 1996). (justia.com)

Resting lymphoid (B and T cells) and myeloid (macrophages, dendritic cells and granulocytes) cells use low levels of glucose oxidative phosphorylation to support "housekeeping" functions. (nih.gov)
In both metabolic disorders, the enzyme deficiencies cause the accumulation of metabolites that are toxic to lymphoid lineage cells. (medscape.com)

In turn T and B cells develop effector functions (cytokine production, cytotoxic function or specific antibody production) associated with a protective immune response that eliminates the offending (or invasive) agent. (nih.gov)
Recent findings have demonstrated that the type and function of immune cells have characteristic metabolic signatures associated with their function. (nih.gov)
In an effort to understand the transformation of a protective immune response into a chronic inflammatory response, we studied the metabolic characteristic of immune cells in cancer and allergic diseases such as asthma. (nih.gov)
NF-κB is a transcription factor that plays a pivotal role in the highly specific pattern of gene expression observed for immune, inflammatory and acute phase response genes, including interleukin 1, interleukin 8, tumor necrosis factor and certain cell adhesion molecules. (justia.com)

Polyploidy has been widely reported to occur in a large variety of plant and animal cells. (plos.org)

In 1952, research further supported the role of chromatolysis in changing the physiology of cells during cell death processes in embryo development. (wikipedia.org)
In particular, I am currently working on primordial germ cell (PGC) migration using the zebrafish embryo as a model organism. (exeter.ac.uk)

In reconstructed cell-free systems, mRNA may be the RNA described in the previous sen- tences (natural mRNA) or any other polyribonucleotide capable of directing, with specificity, the incorporation of amino acids. (nih.gov)
In our view, the experiments carried out with cell-free systems have pro- vided the strongest evidence in support of the concept of mRNA. (nih.gov)
This volume is an essential resource for molecular, cell, and systems biologists who seek to engineer living systems for human benefit. (cshlpress.com)
Large-scale model of mammalian thalamocortical systems. (neurotree.org)

For these experiments, we transfected HEK293 cells with Myc-TDP1 and/or green fluorescent protein (GFP)-SUMO1, followed by immunoprecipitation using anti-Myc antibodies. (nature.com)

Although defects in TDP1 lead to accumulation of SSBs, cell-free studies suggest a requirement of high local concentrations of TDP1 to repair these structures 16 . (nature.com)

Anatomy49

Organisms23

Chemicals and Drugs70

Analytical, Diagnostic and Therapeutic Techniques and Equipment42

Phenomena and Processes71

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science4

Health Care3

Tissues3

Subcellular Fractionation1

Proteins26

Cytotoxicity4

Yeast3

Intracellular3

Nucleus6

Endoplasmic reticulum7

Mechanisms9

Mitochondrial4

Transfection2

Extracellular2

Proliferation4

Tissue3

Broadly1

Biochemical2

Depletion2

Eukaryotic cells1

Cytoplasmic2

Cytoplasm2

Replication1

Biology7

Vitro4

Compartments1

Neurons1

Purification2

Vivo2

Localization2

Stromal cells1

Inhibition4

HeLa2

Proceedings2

Biol Cell2

Stages3

Germ Cells1

Epithelial3

Apoptosis4

Tumor cells2

Nuclear3

19993

Surface antigen1

Antigen3

Receptor2

Apoptotic2

Cellular2

Exosomes1

Fibroblasts1

Acute lymphobl1

Survival1

Protein synthesis1

Process1

Nuclei1

Polarity2

Biological processes1

Measurements2

Cancer Cells2

Tumors5

Analyses2

Pathways3

Methods1

Mechanism1

19961

Lymphoid2

Immune4

Widely1

Embryo2

Systems4

Experiments1

Accumulation1