• Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. (activemotif.com)
  • AbFlex ® MITF antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. (activemotif.com)
  • This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT ® High Sensitivity Kit or our magnetic bead-based ChIP-IT ® Express Kits . (activemotif.com)
  • You will receive recombinant protein and antibody products that meet your particular specifications and expectation. (lifetein.com)
  • You just need to provide the protein sequence, antibody sequence or hybridoma cells, we will provide the proteins or antibodies. (lifetein.com)
  • Sialic acid is usually attached to antibody proteins while they are being assembled in cells, but Anthony wondered if enzymes capable of adding chemical groups like glycans could attach sialic acid to antibodies outside the initial assembly process. (nih.gov)
  • Homeobox protein NOBOX, also known as newborn ovary homeobox protein, is a protein that in humans is encoded by the NOBOX gene. (wikipedia.org)
  • NOBOX is a homeobox gene that is preferentially expressed in oocytes. (wikipedia.org)
  • In addition, most NMD substrates were found to be upregulated upon NMD inactivation, but some were downregulated, pointing to the existence of higher-order NMD targets (or additional functions of the Upf proteins in alternative gene-regulation pathways). (biomedcentral.com)
  • This Human VSIG4 recombinant protein was produced in Mammalian cell, where the gene sequence encoding human VSIG4 (20-283aa) was expressed with the C-terminal 10xHis tag. (cusabio.com)
  • The soluble glutathione transferases (GSTs, EC 2.5.1.18) are encoded by a large and diverse gene family in plants, which can be divided on the basis of sequence identity into the phi, tau, theta, zeta and lambda classes. (biomedcentral.com)
  • The GST proteins have evolved by gene duplication to perform a range of functional roles using the tripeptide glutathione (GSH) as a cosubstrate or coenzyme. (biomedcentral.com)
  • gene to protein expression service in E.coli. (lifetein.com)
  • Just email us a gene sequence or plasmids in any form. (lifetein.com)
  • Cloning of any gene of interest into a bacterial expression vector with His-tag or other selected tag (such as GST, His, FLAG or Thioredoxin). (lifetein.com)
  • We are able to scale up the gene synthesis, plasmid preparation, peptide production or protein expression to hundreds of litres or gram levels. (lifetein.com)
  • 3. What if my gene does not express well? (lifetein.com)
  • My research interest is in the mechanisms of cell differentiation in early mammalian development, in terms of global gene regulatory networks and cascades. (nih.gov)
  • To examine the molecular evolution of NCX genes and understand the role of duplicated genes in the evolution of the vertebrate NCX gene family, we carried out phylogenetic analyses of NCX genes and compared NCX gene structures from sequenced genomes and individual clones. (biomedcentral.com)
  • The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. (lifetein.com)
  • Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV) (Lyttle et al. (cellsystems.eu)
  • S erial Analysis of gene Expression(SAGE), Cap Analysis of gene expression (CAGE), 그리고 Massively Parallel Signature sequencing(MPSS)은 특정 유전자의 발현 양 정보를 얻고자 하는 목표로 수행되는 방법들이다. (insilicogen.com)
  • This protein was also found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. (betalifesci.com)
  • In an effort to facilitate an effective exchange of developments and advancements between pilot centers, the National Institute of General Medical Sciences (NIGMS) organized several workshops on gene cloning, protein expression, and purification. (nih.gov)
  • If the bait and prey fusion proteins physically interact with each other, the activation domain will be brought in proximity to the transcription start site, resulting in transcription of reporter gene(s) and subsequent phenotypic changes of yeast cells. (medscape.com)
  • Tissue and gene enrichment assays showed that the genes that were over-expressed in the CNS were related to functions including neurodevelopment, histone methylation, neurogenesis and synaptic modification. (pharmaceuticalintelligence.com)
  • While lncRNA and protein-coding genes had been co-expressed in 53 lncRNA/protein-coding gene pairs, both had been portrayed just in 4 lncRNA/protein-coding gene pairs differentially, none which included protein-coding genes in overrepresented pathways. (irjs.info)
  • These outcomes recommend lncRNA/protein-coding gene connections play a function mediating hepatic appearance of lipid fat burning capacity/localization and circadian clock genes in response to chronic HFD nourishing. (irjs.info)
  • CREB1 is a member of the cAMP responsive element binding protein family of transcription factors that are critical mediators of gene expression in response to extracellular signals and are essential regulators of adaptive behavior and long-term memory formation (1). (signalchem.com)
  • CREB binds as homo- and heterodimers to promoters containing CRE and activator protein 1 (AP-1) sites to alter target-gene expression). (signalchem.com)
  • They found that many HARs appeared to have the ability to increase the activity of protein-coding genes, while a smaller-but very significant-subset of the HARs appeared to be enhancing gene expression specifically in neural progenitor cells, which are responsible for making various neural cell types. (nih.gov)
  • The amino acid sequences of the homeodomains encoded by genes that originated as copies of the same ancestral gene, which are located in different clusters of genes, are more closely related to one another than the homeodomains en- coded by other genes within the same cluster. (nih.gov)
  • Both the amino acid sequence of the homeodomain encoded by each gene and the order of the genes within the four mammalian Antp-Ubx clusters of genes have been highly conserved during evolution. (nih.gov)
  • 11. Gene expression profiling of human HBV- and/or HCV-associated hepatocellular carcinoma cells using expressed sequence tags. (nih.gov)
  • searched for novel genes involved in early mammalian folliculogenesis in 2002. (wikipedia.org)
  • It is one of the several genes that appeared in the search in expressed sequence tag (EST) databases of mouse. (wikipedia.org)
  • Regulation of these oocyte-specific genes is thru direct binding of NOBOX to its promoter regions via the specific consensus sequences, the NOBOX DNA binding elements (NBEs). (wikipedia.org)
  • The newly assembled Bos taurus genome sequence enables the linkage of bovine milk and lactation data with other mammalian genomes.Using publicly available milk proteome data and mammary expressed sequence tags, 197 milk protein genes and over 6,000 mammary genes were identified in the bovine genome. (nih.gov)
  • Genome location analysis revealed a tendency for milk protein genes to be clustered with other mammary genes. (nih.gov)
  • Approximately 25,000 protein-coding genes are interspersed throughout the genome as discontinuous sets of exons. (ddw-online.com)
  • Expression of genes occurs through transcription - the assembly of continuous, single stranded, messenger ribonucleic acid (mRNA) copies of exons, which are translated into proteins. (ddw-online.com)
  • Single or simultaneous inactivation of the UPF genes stabilizes nonsense-containing mRNAs, indicating that their protein products interact functionally in the same pathway. (biomedcentral.com)
  • 8 ] used high-density oligonucleotide arrays to analyze genome-wide expression profiles of yeast strains containing single deletions of the UPF1 , UPF2 or UPF3 genes, as well as of the DCP1 and XRN1 genes which encode proteins with activities thought to be involved in the NMD pathway - an essential component of the mRNA decapping enzyme and the 5'-3' exonuclease, respectively. (biomedcentral.com)
  • Two-dimensional clustering analysis of the expressed genes for the Δupf1 , Δupf2 and Δupf3 strains yielded several interesting results. (biomedcentral.com)
  • This approach also identified a considerable number of NMD-regulated transcripts (765 out of the 7,839 genes represented on the microarrays) and showed that NMD substrates are generally expressed at below-average levels. (biomedcentral.com)
  • Since that time, a large number of GSTs and GST-like sequences have been cloned from a variety of plants, and in order to make sense of this plethora of genes, a classification system was set up, at first with just three classes, theta, tau and zeta [ 10 ]. (biomedcentral.com)
  • It is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes tyrosinase and tyrosinase related protein 1. (activemotif.com)
  • Your choice of getting synthetic genes, plasmids, peptides or proteins. (lifetein.com)
  • Need more genes, plasmids, peptides or proteins? (lifetein.com)
  • Free codon optimization: Optimized genes have higher protein expression in their correspondent hosts. (lifetein.com)
  • The presence of seven genes encoding NCX homologs may provide teleosts with the functional specialization analogous to the alternate splicing strategy seen with the three NCX mammalian homologs. (biomedcentral.com)
  • 1994). Different isolates of Orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appears to be derived from captured host genes. (cellsystems.eu)
  • The IL-18 receptor belongs to the interleukin-1/Toll-like receptor superfamily, and is encoded by two genes- IL18R1 and IL18RAP -which encode a ligand-binding subunit (commonly known as IL-18R α ) and accessory protein (IL-18R β ) subunit, respectively ( Dinarello, 2009 ). (peerj.com)
  • Beta-hydroxyacid dehydrogenase (β-HAD) genes have been identified in all sequenced genomes of eukaryotes and prokaryotes. (researchgate.net)
  • Furthermore, 5-methylcytosine DNA immunoprecipitation sequencing and targeted bisulfite sequencing uncovered no differential DNA methylation of genes in overrepresented pathways. (irjs.info)
  • In search of clues, researchers have spent years studying the protein-coding genes activated during neurodevelopment. (nih.gov)
  • But some answers may also be hiding in non-coding regions of the human genome, where sequences called regulatory elements increase or decrease the activity of genes. (nih.gov)
  • In the new work, researchers found that about half of the HARs in the human genome influence the activity, or expression, of protein-coding genes in neural cells and tissues during the brain's development [3]. (nih.gov)
  • The data suggest that as the human HAR sequences mutated and diverged from other mammals, they increased their ability to enhance or sometimes suppress the activity of certain genes in neural cells. (nih.gov)
  • Homeobox genes code for proteins that bind to specific nucleotide sequences in DNA and either activate or inhibit the expression of the corresponding genes (for reviews, see refs. (nih.gov)
  • Whereas the Drosophila genome contains only one copy of the Antennapedia (Antp) and Ultrabithorax (Ubx) clusters of homeobox genes, mammalian genomes contain four copies of the combined Antp-Ubx cluster of homeobox genes, which presumably originated by succes- sive duplications of an ancestral cluster of genes (7). (nih.gov)
  • Thus, different combinations of homeobox genes are expressed in different regions along the anterior-posterior axis of the embryo (10, 11). (nih.gov)
  • In this report, the nucleotide sequences of the homeobox regions of Hox-/.1] and Hox-4.9 genes are described. (nih.gov)
  • Multiple species of oligodeoxynucleotides that correspond to highly conserved sequences in the ho- meoboxes of many mouse homeobox genes were synthesized with the aid of an Applied Biosystems DNA synthesizer model 380B and purified by OPC (Applied Biosystems) column chromatography. (nih.gov)
  • For example, nutrient deprivation inhibits the lysosome localized mammalian target of rapamycin complex (mTORC1) and activates the transcription factor TFEB, upregulating multiple lysosomal and autophagy-related genes. (nih.gov)
  • Regarding new therapy approaches, some advances have been achieved by targeting either the vascular endothelial growth factor receptor or the mammalian target of rapamycin ( 5 , 6 ). (spandidos-publications.com)
  • One of the first applications of Generation II sequencing has been maturation of reference genome sequences and variation catalogues, with compilation variant frequencies by sequencing of many human genomes (resequencing) (116-119). (ddw-online.com)
  • Extensive examination of mammalian and avian genomes and synteny analysis argue that NCX4 may be lost in these lineages. (biomedcentral.com)
  • Duplicates for NCX1 , NCX2 , and NCX4 were found in all sequenced teleost genomes. (biomedcentral.com)
  • The most divergent proteins in milk were associated with nutritional and immunological components of milk, whereas highly conserved proteins were associated with secretory processes.Although both copy number and sequence variation contribute to the diversity of milk protein composition across species, our results suggest that this diversity is primarily due to other mechanisms. (nih.gov)
  • Although sequence diversification has produced GSTs with multiple functions, the structure of these proteins has been highly conserved. (biomedcentral.com)
  • Cyclin E1 is a member of the highly conserved cyclin family and belongs to the E-type cyclin that functions as a regulator of S phase entry and progression in mammalian cells. (betalifesci.com)
  • 이번 연재에서는 Next Generation Sequencing의 두 번째 Application인 Expression study 중에 유전자와 엑손의 발현 및 발현된 유전자의 각종 변이 등을 한 번에 연구할 수 있는 RNA-Seq 분석 에 대해 알아보겠습니다. (insilicogen.com)
  • The bovine lactation genome: insights into the evolution of mammalian milk. (nih.gov)
  • Genome sequencing of 2000 canids by the Dog10K consortium advances the understanding of demography, genome function and architecture. (uu.se)
  • Introduction A lot more than 70% from the mammalian genome is normally transcribed as non-coding RNA (ncRNA) while just 1C2% from the mammalian genome is normally transcribed as protein-coding RNA1C3. (irjs.info)
  • All the peptides are derived from the sequence of CaMKII-α, except Peptide H, a peptide that inhibits the kinase domain of CaMKII. (elifesciences.org)
  • The mechanism of apoptosis induced by HDAC inhibitors has not been clearly defined, although it has been recently reported that activation of death receptor pathway ( 6, 7 ), up-regulation of proapoptotic proteins Bmf and Bad ( 8, 9 ), down-regulation of antiapoptotic protein Bcl-2 ( 10 ), and activation of Ku70-inhibited Bax ( 11 ) might be involved in certain types of cancers. (aacrjournals.org)
  • Of the multiple proteins that transiently interact with AMPARs and that determine their trafficking, synaptic targeting and recycling in neurons, some special attention must be given to transmembrane proteins that form integral part of the functional receptor. (frontiersin.org)
  • Previous studies proven safety immunogenicity and efficacy of DNA/altered vaccinia virus Ankara (MVA) perfect/boost vaccines expressing tryparedoxin peroxidase (TRYP) and homologue of the mammalian receptor for activated C kinase (LACK) TH287 against challenge in mice which was consistent with results from TRYP protein/adjuvant combinations in non-human primates. (tech-strategy.org)
  • In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. (lifetein.com)
  • 1999). Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E can not bind to VEGF receptor-1 (Flt-1). (cellsystems.eu)
  • While the mouse and rat transcripts are predicted to encode a truncated receptor with a novel 5 amino acid C-terminal domain, the human sequence is predicted to encode a truncated protein with a novel 22 amino acid sequence bearing resemblance to the 'Box 1' motif of the Toll/interleukin-1 receptor (TIR) domain, in a similar fashion to the inhibitory interleukin-1 receptor 2. (peerj.com)
  • either through associations with IL-18 itself or with its receptor or binding protein. (peerj.com)
  • The translocator protein 18 kDa (TSPO) was previously known as the peripheral benzodiazepine receptor (PBR) in eukaryotes, where it is mainly localized to the mitochondrial outer membrane. (researchgate.net)
  • Translocator protein 18kDa is a mitochondrial outer membrane protein that was first recognized in mammalian systems as the peripheral benzodiazepine receptor (PBR) and in Rhodobacter sphaeroides as the tryptophan-rich sensory protein (TspO). (researchgate.net)
  • ALK-4 (Activin Receptor-Like Kinase 4) or ACVR1B (Activin A Receptor, type 1B), belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family, and TGFB receptor subfamily. (assaygenie.com)
  • The known type II activin receptors include ActRII and ActRIIB, while the main type I activin receptor in mammalian cells is ALK-4 (ActRIB). (assaygenie.com)
  • LncRNAs can boost or decrease appearance of protein-coding transcripts testencodes a higher affinity receptor for the mobile uptake of cholesterylester from high thickness lipoprotein (HDL). (irjs.info)
  • encodes a proteins that mediates degradation from the low-density lipoprotein receptor (LDLR). (irjs.info)
  • Enhanced expression vectors are used to increase the protein expression scale and bioactivity. (lifetein.com)
  • However, the related mouse lipoxygenase mRNA was highly expressed in epidermis of newborn mice and to a lesser extent in adult brain cortex. (nih.gov)
  • Nonsense-mediated mRNA decay (NMD) downmodulates mRNAs that have in-frame premature termination codons and prevents translation of potentially harmful truncated proteins from aberrant mRNAs. (biomedcentral.com)
  • A role for NMD in the regulation of mRNA metabolism beyond the mere vacuum cleaner function for faulty mRNAs has been suspected, and was foreshadowed by work on the splicing factor SC35 and some ribosomal proteins [ 6 , 7 ]. (biomedcentral.com)
  • Cas9 mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9). (trilinkbiotech.com)
  • Cas9 mRNA encodes the Cas9 protein with an N and C terminal nuclear localization signal (NLS). (trilinkbiotech.com)
  • to include a cDNA sequence corresponding to a splice variant of IL18r1 , formed by inclusion of part of an unspliced intron in the mRNA sequence. (peerj.com)
  • FLAG ® and 3xFLAG ® reagents have proven utility in numerous applications such as Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, the study of protein-protein interactions, cell ultrastructure and protein localization. (sigmaaldrich.com)
  • Here, using co-immunoprecipitation and mass spectrometry, we identified hundreds of potential candidate MPS-interacting proteins that span diverse functional categories. (nature.com)
  • We have conducted crosslinking, immunoprecipitation, and sequencing (CLIP-seq) to characterize N interactions with host and viral RNAs during infection. (mdpi.com)
  • Among the techniques available, co-immunoprecipitation (co-IP) is a widely used conventional method for identifying and verifying protein-protein interactions. (medscape.com)
  • Tandem affinity purification is one of the most robust immunoprecipitation techniques in which the protein of interest is fused with a tandem affinity purification tag that allows for highly efficient detection and purification. (medscape.com)
  • The FLAG ® and 3xFLAG ® vectors are available for bacterial and mammalian expression, using T7, Tac or CMV promoters. (sigmaaldrich.com)
  • Vectors have an enterokinase cleavage site for removal of the tag from purified protein. (sigmaaldrich.com)
  • Evidence is presented that scylla and charybdis , like some of their mammalian homologs, are induced by hypoxia and that Scylla and Charybdis act as partially redundant negative regulators of growth by controlling S6K but not PKB activity (Reiling, 2004). (sdbonline.org)
  • epithelial cells of the small intestine and kidney also express these enzymes to a lesser extent, but their contribution to urea production is not significant. (medscape.com)
  • Here, we report that the expression status of adenomatous polyposis coli (APC) protein determines the relative sensitivity of colon cancer cells to HDAC inhibitor-induced apoptosis. (aacrjournals.org)
  • HCA-7 cells (expressing wild-type β-catenin and APC proteins) are more sensitive to apoptosis induced by HDAC inhibitors valproic acid (VPA) and suberoylanilide hydroxamic acid than SW620 or HT-29 cells (both expressing mutant APC). (aacrjournals.org)
  • When wild-type APC protein was expressed using an inducible expression system, HT-29 cells became sensitive to apoptosis in response to VPA. (aacrjournals.org)
  • Conversely, knocking down of endogenous APC protein by small interfering RNA (siRNA) blocked VPA-induced apoptosis in HCA-7 cells. (aacrjournals.org)
  • The level of survivin protein decreased in HCA-7 and HT-29/APC cells, but not in SW620 and HT-29/β-Gal cells after VPA treatment. (aacrjournals.org)
  • VPA also induced proteasome-mediated degradation of survivin protein in HCA-7 cells. (aacrjournals.org)
  • This transporter is predominantly expressed in the disc membranes of photoreceptor cells in the retina where it plays a crucial role. (ubc.ca)
  • Single-molecule fluorescence intensity analysis of CaMKII-α expressed in mammalian cells shows that activation of CaMKII-α results in the destabilization of the holoenzyme. (elifesciences.org)
  • This work identifies Drosophila S2 cells as a clinically-relevant platform suited for the production of 'difficult-to-make' proteins from Plasmodium parasites, and identifies a PfRH5 sequence variant that can be used for clinical production of a non-glycosylated, soluble full-length protein vaccine immunogen. (nature.com)
  • To try and get around this problem recombinant spider silk proteins have been produced in hosts such as bacteria, yeast, baculovirus/insect systems, mammalian cells, and transgenic plants and animals. (genengnews.com)
  • Unique sequences encoded within the vector construct contain promoter elements that are recognized by the transfected cells own protein synthesis machinery to drive the expression of the incorporated protein sequence. (sigmaaldrich.com)
  • Both the 125I‑labelled EVD ( 125 I‑EVD) and 99mTc‑labelled EVD ( 99m Tc‑EVD) bound specifically to EpCAM‑expressing SK‑RC‑52 renal carcinoma cells. (spandidos-publications.com)
  • [ 29 ] The major advantage of yeast two-hybrid is that it is an in vivo method in which both the protein expression and the selection for protein-protein interaction occur in eukaryotic yeast cells. (medscape.com)
  • Truncated versions of IRP2 were expressed in H1299 cells and analyzed for their response to iron. (biomedcentral.com)
  • However, the fusion of C-terminal IRP2 fragments to luciferase failed to sensitize the indicator protein for degradation in iron-loaded cells. (biomedcentral.com)
  • Recombinant rat CREB1 (1-280) was expressed in E. coli cells using a N-terminal GST tag. (signalchem.com)
  • Activation of CREB1 by protein kinase A-mediated phosphorylation has been implicated in the survival of mammalian cells. (signalchem.com)
  • 1. Quantitative proteomic signature of liver cancer cells: tissue transglutaminase 2 could be a novel protein candidate of human hepatocellular carcinoma. (nih.gov)
  • 16 , 17 In particular, transgenic zebrafish lines that express nfsB in β cells, cardiomyocytes, or other cell types have been established, and their exposure to the metronidazole leads to the targeted loss of these cells. (cdc.gov)
  • databases agreed to adopt the NCBI taxonomy as the standard classification for the nucleotide sequence databases. (nih.gov)
  • In addition, nucleotide sequence analysis of the homeobox regions of cloned Hox-4.3 and Hox-4.2 genomic DNA revealed some differences in nucleotide sequences and in the deduced homeodomain amino acid sequences compared with the sequences that have been reported. (nih.gov)
  • These small hydrophilic tags facilitate superior detection and purification of recombinant fusion proteins when using our highly specific and sensitive ANTI-FLAG ® antibodies. (sigmaaldrich.com)
  • Following the purification and cloning of GSTs active in herbicide detoxification in maize in the 1980s [ 9 ], it quickly became apparent that the plant enzymes differed significantly in sequence from their mammalian counterparts [ 10 ]. (biomedcentral.com)
  • Additionally, a C terminal HA epitope tag aids detection, isolation, and purification of the Cas9 protein. (trilinkbiotech.com)
  • Discussions focused on progress in high-throughput methods for cloning, production, purification, and crystallization of proteins for X-ray crystallography and NMR studies. (nih.gov)
  • B efore discussing specific areas of drug discovery and development that we anticipate will be profoundly impacted by Generation II (or NextGen) sequencing, we first review current knowledge of nucleic acid variation, its functional correlates, and the current technical capabilities of Generation II sequencers. (ddw-online.com)
  • We sought to investigate the structure-function relationship of SNAP-25 in functional mammalian CNS synapses by combining the specificity of clostridial neurotoxin cleavage with the ability to transfect organotypic hippocampal slices with mutated forms of SNAP-25. (jneurosci.org)
  • Genetic perturbations of the MPS and its interacting proteins further suggested functional roles of the MPS in axon-axon and axon-dendrite interactions and in axon diameter regulation, and implicated the involvement of MPS interactions with cell adhesion molecules and non-muscle myosin in these roles. (nature.com)
  • Mammalian expression systems can be used when a target protein with posttranslational modifications is needed for functional and structural studies. (lifetein.com)
  • In the adult mammalian heart, NCX expression levels increase during heart failure and arrhythmias as an intrinsic mechanism of cardiac functional compensation, though with potentially negative side-effects [ 5 ]. (biomedcentral.com)
  • You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. (betalifesci.com)
  • Voltage-gated Na + channel β1 ( Scn1b ) subunits are multi-functional proteins that play roles in current modulation, channel cell surface expression, cell adhesion, cell migration, and neurite outgrowth. (biomedcentral.com)
  • Voltage gated Na + channel β1 ( Scn1b ) subunits are multi-functional proteins that participate in inter- and intra-cellular communication on multiple time scales via modulation of electrical signal transduction and cell adhesion [ 1 , 2 ]. (biomedcentral.com)
  • As with other mitochondrial carrier family proteins, the ornithine carrier is composed of 300 amino acids that constitute 3 repeated motifs of approximately 100 amino acids each. (medscape.com)
  • The transporter was identified by probing a mammalian-expressed sequence tag database with 2 fungal mitochondrial ornithine carrier protein sequences. (medscape.com)
  • In addition to the dimeric soluble GSTs, other proteins have been identified as having a restricted ability to conjugate xenobiotics (foreign organic compounds) with GSH, notably the distantly related mitochondrial kappa GSTs and the trimeric microsomal GSTs of animals [ 4 , 5 ]. (biomedcentral.com)
  • Ca 2+ /calmodulin-dependent protein kinase II (CaMKII) is an oligomeric enzyme with crucial roles in neuronal signaling and cardiac function. (elifesciences.org)
  • Although chIL-36RN exhibits low homology with its mammalian orthologues, it encodes a predicted beta-trefoil structure whose beta-strands are conserved Citarinostat molecular weight with those of the mouse sequence. (peptidesolubility.com)
  • NCX is expressed in virtually all tissues across a phylogenetically diverse group of species. (biomedcentral.com)
  • 2005) Comparative analyses of six solanaceous transcriptomes reveal a high degree of sequence conservation and species-specific transcripts. (insilicogen.com)
  • These motifs contain 2 hydrophobic alpha-helical segments connected by an extensive hydrophilic sequence, resulting in 6 transmembrane portions of the protein. (medscape.com)
  • Both scn1ba splice forms modulate Na + currents expressed by zebrafish scn8aa , resulting in shifts in channel gating mode, increased current amplitude, negative shifts in the voltage dependence of current activation and inactivation, and increases in the rate of recovery from inactivation, similar to the function of mammalian β1 subunits. (biomedcentral.com)
  • In contrast to mammalian β1, however, neither zebrafish subunit produces a complete shift to the fast gating mode and neither subunit produces complete channel inactivation or recovery from inactivation. (biomedcentral.com)
  • We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)
  • Mammalian vector with a CMV promoter for expressing N-terminally Myc-tagged proteins. (snapgene.com)
  • Following initial ex vivo tests to confirm that the chimeric fusion protein was expressed in posterior silk glands, the piggyBac vector was mixed with a plasmid encoding the piggyBac transposase and injected into eggs isolated from a strain of the B. mori silk moth that has a melaninization deficiency to facilitate detection of the EGFP-tagged chimeric silkworm/spider silk protein in transformed larvae. (genengnews.com)
  • Because of its hydrophilic nature, the FLAG ® peptide is likely to be located on the surface of the fusion protein. (sigmaaldrich.com)
  • In addition, because of the small size of the FLAG ® peptide tag, it is not likely to obscure other epitopes, domains, or alter function, secretion, or transport of the fusion protein. (sigmaaldrich.com)
  • Rat liver CDO was cloned and expressed in E. coli as a 26.8 kDa fusion protein bearing a poly-histidine tag. (umass.edu)
  • GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. (cellsystems.eu)
  • The coding sequence of SNAP-tag was cloned into a pTXB1 derived E. coli T7 expression vector. (neb.com)
  • Order your E.coli Protein Service at a competitive price! (lifetein.com)
  • [ 31-33 ] In phage display, the proteins in the library are expressed in host bacteria, thus transformation is mandatory. (medscape.com)
  • Rommie Amaro, a 2010 New Innovator, discovered a protein that may lead to a vaccine for the bacteria group A Streptococcus, a leading cause of worldwide morbidity and mortality and responsible for infections and diseases such as pharyngitis, rheumatic heart disease, and necrotizing fasciitis ("flesh-eating" disease). (nih.gov)
  • The FLAG ® expression system utilizes a short, hydrophilic 8-amino acid peptide that is fused to the recombinant protein of interest. (sigmaaldrich.com)
  • While peptide synthesis is usually for difficult proteins that are not feasible within any protein expression system. (lifetein.com)
  • Another well-established technology for the selection of peptide or protein sequences with desired properties is phage display. (medscape.com)
  • This method uses bacteriophage to noncovalently couple a protein with its coding cDNA by displaying the peptide of interest as a fusion with a coat protein on the surface of filamentous phage. (medscape.com)
  • A, aligned amino acid sequences of several GLP-1R peptide ligands. (phoenixpeptide.com)
  • These findings are consistent with studies using TRYP protein/adjuvant mixtures in mice and non-human primates [15]. (tech-strategy.org)
  • Thus, we analyzed and compared differentially expressed mice genital tract miRNAs after single and repeat chlamydia infections using a C. muridarum mouse model. (biomedcentral.com)
  • EC 2.5.1.18) are soluble proteins with typical molecular masses of around 50 kDa, and each is composed of two polypeptide subunits. (biomedcentral.com)
  • Under normoxic conditions, HIF α subunits undergo post-translational modification by the prolyl-hydroxylases PHD1-3, which tag them for ubiquitination by the E3 ubiquitin ligase VHL and degradation by the proteasome [ 21 ]. (biomedcentral.com)
  • Iron regulatory protein 2 (IRP2), a post-transcriptional regulator of cellular iron metabolism, undergoes iron-dependent degradation via the ubiquitin-proteasome pathway. (biomedcentral.com)
  • Moreover, the replacement of domain 4 of IRP1 with the corresponding region of IRP2 sensitized the chimerical IRP1 1-3 /IRP2 4 protein to iron-dependent degradation, while the reverse manipulation gave rise to a stable chimerical IRP2 1-3 /IRP1 4 protein. (biomedcentral.com)
  • Also, the number of miRNAs expressed each week after chlamydia infection and reinfection varied, with weeks eight and one having the highest number of differentially expressed miRNAs for chlamydia infection and reinfection respectively. (biomedcentral.com)
  • The antibodies induced by one protein variant were shown to be qualitatively similar to responses induced by other vaccine platforms. (nature.com)
  • All proteins were detected using the appropriate tag antibodies, anti-mouse HRP and ECL. (sigmaaldrich.com)
  • The FLAG ® and 3xFLAG ® sequences include the binding sites for several highly specific ANTI-FLAG ® monoclonal (M1, M2, M5) and polyclonal antibodies and conjugates, each with different recognition and binding characteristics. (sigmaaldrich.com)
  • ANTI-FLAG ® antibodies exhibit little or no cross-reactivity in most mammalian and bacterial cell lysates. (sigmaaldrich.com)
  • AbFlex ® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. (activemotif.com)
  • AbFlex ® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. (activemotif.com)
  • However it has proved challenging to identify a heterologous expression platform that can produce a soluble protein-based vaccine in a manner compliant with current Good Manufacturing Practice (cGMP). (nature.com)
  • Synaptic vesicle fusion is driven by the formation of a four-helical bundle composed of soluble N- ethylmaleimide sensitive factor (NSF) attachment protein receptors (SNAREs). (jneurosci.org)
  • Recombinant Protein Expression Services: Bacterial and mammalian cell protein service. (lifetein.com)
  • LifeTein's custom bacterial protein expression platform is optimized for low production costs, high scalability, and attractive low pricing for the production of recombinant protein. (lifetein.com)
  • Selected phages containing fusion proteins with desired properties are used to infect a suitable bacterial host, from which the phagemids can be collected and the relevant DNA sequences amplified for the next round of selection or sequenced and analyzed. (medscape.com)
  • The 6H-rNT protein was expressed in a bacterial system and lacks glycosylation but contains a His6 tag (HHHHHH) close to the N terminus. (phoenixpeptide.com)
  • Cysteine dioxygenase (CDO) catalyzes the oxidation of cysteine to cysteine sulfinic acid, which is the first major step in cysteine catabolism in mammalian tissues. (umass.edu)
  • The 18-kilodalton translocator protein (TSPO), proposed to be a key player in cholesterol transport into mitochondria, is highly expressed in steroidogenic tissues, metastatic cancer, and inflammatory and neurological diseases such as Alzheimer's and Parkinson's. (researchgate.net)
  • The miRNAs expression profile was different in the reproductive tissues after a reinfection, with a greater number of miRNAs expressed after reinfection. (biomedcentral.com)
  • Inheritance, homology by common descent, and the conservation of sequence and structure in the determination of function are all central ideas in biology that are directly related to the evolutionary history of any group of organisms. (nih.gov)
  • Understanding the mutations in NOBOX homeodomain is important to researchers and clinicians to develop diagnostic and therapeutic approaches for POF such as genetic control of mammalian reproductive life-span, regulation of fertility, and generation of mature eggs in the lab. (wikipedia.org)
  • Leveraging base-pair mammalian constraint to understand genetic variation and human disease. (uu.se)
  • Viral origins contain a specific core DNA sequence that is required for initiation to proceed efficiently, whereas the requirement for specific DNA sequences is greatly diminished in single cell eukaryotes and virtually absent in multicellular eukaryotes. (nih.gov)
  • Thus, origins in multicellular eukaryotes are more similar to those in S. pombe than to those in S. cerevisiae in terms of their size, sequence complexity, and their lack of a required consensus sequence. (nih.gov)
  • Why sequence all eukaryotes? (uu.se)
  • Iron regulatory proteins, IRP1 and IRP2, post-transcriptionally control the expression of several mRNAs bearing iron responsive elements (IREs). (biomedcentral.com)
  • We envisioned that enzyme-activatable cMOs could overcome these limitations, as the triggering enzymes could be expressed in individual cell populations using cis regulatory elements. (cdc.gov)
  • See Fig. 2 for the nucleotide sequences of the primers. (nih.gov)
  • 2003) Comparative analyses of potato expressed sequence tag libraries. (insilicogen.com)
  • [ 28 , 29 ] The libraries of the proteins used in yeast two-hybrid are typically from natural sources, such as those based on cDNAs of humans or model organisms. (medscape.com)
  • We have different expression strains and leader sequences for optimizing expression. (lifetein.com)
  • Streptokinases secreted by nonhuman isolates of group C streptococci (strains which activated either equine or porcine plasminogen were cloned, sequenced, and expressed in isolates but also these proteins participate in a category of plasminogen activators even more diverse than previously idea. (buyresearchchemicalss.net)
  • The human NOBOX is a 14 kb protein and encoded by 8 exons. (wikipedia.org)
  • Expressed sequence tag information was used to clone the full-length sequence for a new human lipoxygenase from the B cell line CCL-156. (nih.gov)
  • A related mouse sequence with 83% nucleotide identity to the human sequence was also cloned. (nih.gov)
  • The human lipoxygenase, when expressed via the baculovirus/insect cell system produced an approximately 80-kDa protein capable of metabolizing arachidonic acid to a product identified as 12-hydroxyeicosatetraenoic acid by mass spectrometry. (nih.gov)
  • The Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRH5) has recently emerged as a leading candidate antigen against the blood-stage human malaria parasite. (nature.com)
  • Early embryos undergoing rapid cell cleavage (e.g. frogs, flies, sea urchin, and fish) initiate DNA replication with no obvious requirement for any specific DNA sequences, and Drosophila ORC or human ORC can replace Xenopus ORC in frog egg extracts with the same result. (nih.gov)
  • Our study provides new insights into the structure and function of RS3 in mammalian spermatozoa and the molecular pathogenicity of LRRC23 underlying reduced sperm motility in infertile human males. (elifesciences.org)
  • Recombinant protein of the extracellular domain (Gln18-Asp615) of Homo sapiens angiotensin converting enzyme 2 (ACE2), transcript variant 2 (NM_021804), with H374N, H378N point mutation, and a Human IgG1 Fc tag. (lifetein.com)
  • The first somatic mammalian hyaluronidase isolated to homogeneity from a mammalian source was Hyal-1, which was obtained from human plasma. (gr.jp)
  • A DNA sequence encoding the rat ACVR1B (Met1-Glu126) was expressed with the Fc region of human IgG1 at the C-terminus. (assaygenie.com)
  • All eight cysteine residues of the central cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins (Dehio et al. (cellsystems.eu)
  • Therefore, the risk of altering protein function, blocking other epitopes or decreasing solubility is minimized. (sigmaaldrich.com)
  • Optimized refolding methodology is available to solve the protein solubility issues. (lifetein.com)
  • The designed ankyrin repeat protein, Ec1, is a small (molecular weight, 18 kDa) targeting protein with a subnanomolar affinity to EpCAM. (spandidos-publications.com)
  • Using a modified Ec1, a tracer was developed for the radionuclide‑based visualization of EpCAM in vivo , i.e., an EpCAM‑visualizing designed ankyrin repeat protein (EVD). (spandidos-publications.com)
  • The C-terminus of Scn1ba_tv1 contains a tyrosine residue similar to that found to be critical for ankyrin association and Na + channel modulation in mammalian β1. (biomedcentral.com)
  • Klebsiella produces two ARD enzymes that share a common polypeptide sequence and differ only in the metal ion present. (umass.edu)
  • In yeast, NMD depends on the expression of the Upf1, Upf2 (Nmd2) and Upf3 proteins. (biomedcentral.com)
  • Origins in the fission yeast Schizosaccharomyces pombe lack a genetically required consensus sequence, although they do exhibit "autonomously replicating sequence" (ARS) activity. (nih.gov)
  • The yeast two-hybrid system is widely used to screen for protein-protein interactions with numerous successes. (medscape.com)
  • In yeast two-hybrid, the DNA-binding domain is fused with a target protein of interest as the bait, while the activation domain is fused with a library of known or unknown proteins as the prey. (medscape.com)
  • A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 (Dehio et al. (cellsystems.eu)
  • named this variant type II IL18r1 , with the reference sequence inheriting the nomenclature type I IL18r1 , and presented evidence for its expression in the mouse brain. (peerj.com)
  • We showed that RapA forms a stable complex with RNAP and that binding to RNAP stimulates its ATPase activity, thus demonstrating that the two proteins interact physically as well as functionally. (nih.gov)
  • Thus, NMD serves as a 'cellular vacuum cleaner' that protects the cell from the potentially harmful effects of truncated proteins by eliminating mRNAs with PTCs in a sequence of events that is not yet fully understood. (biomedcentral.com)
  • Here we report the production of full-length PfRH5 protein using a cGMP-compliant platform called ExpreS 2 , based on a Drosophila melanogaster Schneider 2 (S2) stable cell line system. (nature.com)
  • highly specific proteases that cleave individual SNARE proteins, have been used extensively as a powerful tool to examine the function of toxin-cleaved SNARE proteins during neuromuscular or CNS synaptic transmission, as well as for chromaffin cell secretion. (jneurosci.org)
  • We examined representative proteins in several of these categories using super-resolution imaging, including previously unknown MPS structural components, as well as motor proteins, cell adhesion molecules, ion channels, and signaling proteins, and observed periodic distributions characteristic of the MPS along the neurites for ~20 proteins. (nature.com)
  • It also protected protein thiols and increased cell survival. (peptidesolubility.com)
  • These are predominantly situated in the cell nucleus and expressed at lower levels than protein-coding RNAs usually. (irjs.info)
  • Using CaptureMPRA technology in tandem with cell culture studies, researchers rolled up their sleeves and conducted comprehensive, full-sequence analyses of more than 3,000 HARs. (nih.gov)
  • Like the original FLAG ® tag, 3xFLAG ® is hydrophilic, contains an Ek cleavage site, and is relatively small. (sigmaaldrich.com)
  • The cleavage site for Ex-4 contains the consensus sequences for cleavage by furin, PC1, and/or PC2. (phoenixpeptide.com)
  • The consensus target DNA recognition sequence is the same as the native enzyme and shows minimal insertional sequence bias. (nih.gov)
  • When new sequences are submitted to GenBank, the submission is checked for new organism names, which are then classified and added to the Taxonomy database. (nih.gov)
  • The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. (cusabio.com)
  • It improves protein stability, extends storage time, and reduces shipping costs. (betalifesci.com)
  • Indeed, charybdis but not scylla is expressed in neuromuscular junctions. (sdbonline.org)