Mammalian cho cells. Medical search. Frequent questions

These include bacteria, yeast, and mammalian cells such as CHO. (2bscientific.com)
Other systems are used in commercial processes but are less common, including insect cells, yeast, algae, and cell-free expression platforms ( Markets and Markets, 2017 ). (frontiersin.org)
New vector for transfer of yeast artificial chromosomes to mammalian cells. (ox.ac.uk)
A modification vector has been constructed to facilitate the transfer of yeast artificial chromosomes (YACs) to mammalian cells in culture by targeting a dominant selectable marker (G418 resistance) to the right arm of pYAC4 clones. (ox.ac.uk)

In a cytogenicity study in mammalian cells, conducted according to OECD Test Guideline 473 and in compliance with GLP, the analogous substance dichloro(3-chloropropyl)methylsilane (CAS 7787-93-1) was negative in Chinese hamster fibroblast cells (Hüls AG, 1997, Reliability Score 1). (europa.eu)

Optimisation de la croissance de cellules HEK293 exprimant la pyruvate carboxylase dans un bioréacteur en mode cuvée-alimentée [Master's thesis, École Polytechnique de Montréal]. (polymtl.ca)
Hek293, CHO, others? (cellculturedish.com)

An in vitro mammalian chromosome aberration test was performed with Castor oil (CAS No. 8001-79-4) in Chinese hamster Ovary (CHO) cells (Irwin, NTP report 1992). (europa.eu)
The requirements for the successful culture of organoids in vitro differ significantly from those of traditional monolayer cell cultures. (mdpi.com)
Desirable attributes of a CHO cell line include its ability to produce high titers of biotherapeutic proteins facilitate quick recoveries and selection processes maintain phenotypic and genetic stability throughout in-vitro aging of a culture. (bioprocessintl.com)
The bacteria-produced GM-CSF stimulated the maturation of bone marrow-derived dendritic cells (BMDCs), while IL-7 promoted the proliferation of spleen isolated T cells and inhibited cytotoxicity T cell apoptosis in vitro. (bvsalud.org)
The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION. (bvsalud.org)

Cytogenicity in mammalian cells: negative in V79 CHL cells (OECD TG 473) (Degussa-Hüls AG, 1999). (europa.eu)

Non-Mammalian Cells (Bacteria, etc. (metabolon.com)
Moreover, the tumor-toxic GZMB+ CD8+ T cell and IFN-Î³+ CD8+ T cell populations conspicuously increased with the treatment of engineered bacteria. (bvsalud.org)

Prior to assay set-up, CHO cells were grown to between 70-90% confluence in T75 tissue culture flasks in 10-20 mL sterile culture media, and spun and detached using trypsin. (genengnews.com)
Using the SURVEYOR nuclease assay 13 , we assessed the ability of each Cas9-sgRNA complex to generate indels in human embryonic kidney (HEK) 293FT cells through the induction of DNA doublestranded breaks (DSBs) and subsequent nonhomologous end joining (NHEJ) DNA damage repair (Online Methods). (cdc.gov)

To provide high-density cell cultures with sufficient iron, elevated concentrations of iron salts are required that utilize low-affinity nontransferrin receptor pathways. (genengnews.com)
Current understanding of mammalian cell cultures lies in the association of their metabolic behaviour to their antibody production output. (imperial.ac.uk)
Intensification of influenza virus production in fed-batch and perfusion cultures of HEK293SF cells [Paper]. (polymtl.ca)
Cell cultures derived from mammalian and bacterial cell lines are the conventional production systems in bioprocessing. (bioprocessintl.com)
Media for mammalian cultures in particular are notoriously expensive, and traditional cell cultures can be highly sensitive to growing conditions. (bioprocessintl.com)
During the late 1980s and into the 1990s, plants and plant-derived cell cultures were introduced as alternative cell-culture systems (1, 2). (bioprocessintl.com)
One of the main problems in mammalian cell culture systems, including Chinese Hamster Ovary (CHO) cultures, is the inability to maintain viability of these cultures over extended periods of time. (mit.edu)
This thesis was undertaken to better understand the processes of death in CHO cells and to find ways to extend the viability of CHO cultures. (mit.edu)
In contrast, expression of bcl- 2, a well-characterized anti-apoptotic gene, was able to significantly increase the life of CHO batch cultures in response to both glucose limitation and growth factor withdrawal. (mit.edu)
Bcl-2 expression was shown to improve both final product titers and integrated viable cell densities in CHO fed-batch cultures. (mit.edu)
Concentrations of insulin, a growth and survival factor used in CHO culture, were observed to drop rapidly in fed-batch cultures of CHO cells. (mit.edu)
Insulin degradation was sharply reduced by adding sodium glycocholate, an amino-peptidase inhibitor, suggesting that amino-peptidases play a major role in insulin degradation in CHO fed-batch cultures. (mit.edu)
Based on the above results, possible ways to further reduce death and improve productivity in CHO cultures are also suggested. (mit.edu)

We demonstrate that relatively short single-stranded oligodeoxynucleotides, 25-61 bases homologous to the target sequence except for a single mismatch to the targeted base, are capable of correcting a single point mutation (G to A) in the mutant beta-galactosidase gene, in nuclear extracts, episome, and chromosome of mammalian cells, with correction rates of approximately 0.05%, 1% and 0.1%, respectively. (nih.gov)
In conclusion, Castor oil did not induce chromosome aberrations in Chinese hamster Ovary cells, neither in the presence nor in the absence of a metabolic activation system, under these experimental conditions. (europa.eu)
In a chromosome aberration test in CHO-WBL cells, structural (with metabolic activation) and numerical (without metabolic activation) aberrations were reported after 3-hour treatment at concentrations up to cytotoxicity. (europa.eu)

Chinese hamster ovary (CHO) cells are an epithelial cell line derived from the ovary of the Chinese hamster, often used in biological and medical research and commercially in the production of recombinant therapeutic proteins. (wikipedia.org)
This genetic selection scheme remains one of the standard methods to establish transfected CHO cell lines for the production of recombinant therapeutic proteins. (wikipedia.org)
It is expected research in this area will lead to improved engineering strategies for developing cell lines for higher titers of desired recombinant proteins, including relevant therapeutic protein products. (aiche.org)
Recombinant therapeutic protein expression using mammalian platforms can be achieved using a range of workflows optimized for different objectives. (lonza.com)
Identifying these changes can help us understand how diseases such as epilepsy affect these cells and may represent viable therapeutic targets. (dcu.ie)
Mammalian cells are mostly used in the biopharmaceutical industry for the production of recombinant therapeutic proteins. (medgadget.com)
Increasing demand for cell-based therapies: The use of cells as a therapeutic agent is a promising area of research, with several cell-based therapies already approved for use in clinical settings. (medgadget.com)
Therapeutic monoclonal antibodies (MAbs) mostly are manufactured using bioengineered mammalian cells cultured in a bioreactor for two to three weeks. (bioprocessintl.com)
However, patients suffered with tumors that had inadequate infiltrated immune cells only receive limited therapeutic benefits from ICB therapy. (bvsalud.org)
Fibrous papule of the face, similar to tuberous sclerosis complex-associated angiofibroma, shows activation of the mammalian target of rapamycin pathway: evidence for a novel therapeutic strategy? (medscape.com)

do not let your cells overgrow and do not "over split" your cells by sub culturing the cells at low densities on multiple consecutive days. (cellculturedish.com)

Mutagenicity in mammalian cells: negative in CHO K-1 cells (OECD TG 476) (Hüls AG, 1998b). (europa.eu)

Since then, CHO cells have been a cell line of choice because of their rapid growth in suspension culture and high protein production. (wikipedia.org)
Due to this, CHO cells lacking DHFR are the most widely used CHO cells for industrial protein production. (wikipedia.org)
In order to measure ER stress in cell lines engineered for high-levels of recombinant protein production, this research aims to demonstrate the presence of ER stress associated with immunoglobulin G (IgG) antibody production, elucidate the progression of UPR in response to highly producing recombinant protein, and determine the effect of UPR on product quality. (aiche.org)
There are also several platforms based on plants and plant cells, but these have not been included in the latest market studies, indicating they have not yet commanded a significant share of commercial protein production capacity. (frontiersin.org)

Cells used for transient protein expression should be meticulously maintained from the time of thaw throughout their useful lifespan. (cellculturedish.com)

Moreover, mammalian cell lines are used as host cells for the production of biologics in the biopharmaceutical industry. (medgadget.com)
With 20 years of experience in the biopharmaceutical industry - at Genentech, Applied Biosystems, Cell Genesys, Cellerant Therapeutics, and Bayer - Yuval Shimoni has written frequently for BioProcess International on a number of production topics. (bioprocessintl.com)

It delivers exceptional transfection efficiency with improved cell viability for the widest range of hard-to-transfect and common cell types including HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines (Figure 1) . (thermofisher.com)
Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection. (thermofisher.com)
Each reagent was used to transfect HeLa cells in a 96-well format at the indicated doses with an emerald green fluorescent protein (GFP)-expressing vector. (thermofisher.com)

These processes include transient transfection, and the creation of stable pools and clonal cell lines. (lonza.com)
Transient and stable pool processes are generally used for discovery through early-stage development to enable rapid production and screening of large numbers of protein candidates, while stable clonal cell lines are used for GMP clinical and commercial manufacturing. (lonza.com)
These EVs can mediate signalling to cells throughout the body, affecting major cell processes. (dcu.ie)
Models of animal cell culture systems have a wide range of potential applications, such as analysis and prediction of experimental results, optimisation of culture conditions for prolonged viability, and perhaps most importantly, the investigation of fundamental metabolic processes and their subsequent elucidation. (imperial.ac.uk)
The integration of these three research tools represents a unique, novel, and interdisciplinary approach to addressing the complicated research and industrial problem of model-based control and optimisation of mammalian cell culture processes. (imperial.ac.uk)
Modern Sensor Tools and Techniques for Monitoring, Controlling, and Improving Cell Culture Processes. (polymtl.ca)

The process begins with the molecular cloning of the gene of interest and the DHFR gene into a single mammalian expression system. (wikipedia.org)
NewBiologix uses next-generation sequencing (NGS)-based genome characterisation to develop a proprietary bioinformatics platform designed to improve cell line capabilities for the expression and production of ATMPs. (ddw-online.com)
Commonly used cell lines include Chinese hamster ovary (CHO) mammalian cell expression systems, such as CHOK1. (iptonline.com)
This allows the biopharma industry to make real, substantive gains in cell selection control and achieve enhanced levels of expression. (iptonline.com)
AQP4 operates in complex with the potassium channel Kir4.1, and their cell surface expression is mediated through the dystrophin-associated protein complex (DAPC) and the extracellular matrix. (dcu.ie)
Until recently, T-cells were divided into well-defined subsets, identified by the expression of a key cytokine. (dcu.ie)
Mammalian cells are currently the preferred expression system for high-value bio-pharmaceutical products, in particular, monoclonal antibodies. (imperial.ac.uk)
Over the last three decades, the expression of recombinant proteins in plants and plant cells has been promoted as an alternative cost-effective production platform. (frontiersin.org)
However, the market is still dominated by prokaryotic and mammalian expression systems, the former offering high production capacity at a low cost, and the latter favored for the production of complex biopharmaceutical products. (frontiersin.org)
Comprehensive Characterization of an Inducible Mammalian Expression System Using 13C-Metabolic Flux Analysis [Ph.D. thesis, École Polytechnique de Montréal]. (polymtl.ca)
In addition, the experiments proved that bcl-2 expression is able to extend the replication competence of cells under normal culture conditions. (mit.edu)
The loss of insulin was concurrent with the accumulation of cells in the GO/G1 state and an increase in expression levels ofp53, a well-documented growth-inhibiting and apoptosis-inducing gene. (mit.edu)
A study from 2014 showed expression of antibodies against p-mTOR in dermal stromal cells and epidermal keratinocytes in fibrous papules, similar to tuberous-sclerosis complex-associated facial angiofibromas, suggesting topical rapamycin may be a treatment option. (medscape.com)
Clear cell fibrous papule with NKI/C3 expression: clinical and histologic features in six cases. (medscape.com)

Cell viability was determined by the trypan blue exclusion method. (genengnews.com)
At the time of transfection, CHO cells, like most other cell types, should have high viability (95% or greater) and should be in low to mid log phase growth. (cellculturedish.com)

All CHO cell lines are deficient in proline synthesis. (wikipedia.org)
Cell lines with mutated DHFR are useful for genetic manipulation as cells transfected with a gene of interest along with a functional copy of the DHFR gene can easily be screened for in thymidine-lacking media. (wikipedia.org)
Most, if not all industrially used CHO cell lines are now cultivated in animal component free media or in chemically defined media, and are used in large scale bioreactors under suspension culture. (wikipedia.org)
however , secretion levels needed for industrial cell lines likely leads to an imbalance in ER homeostasis, resulting in increased cellular stress. (aiche.org)
It is a recombinant analog of human transferrin that has shown equivalence to serum-derived human transferrin (hTf) and superiority to bovine transferrin (bTf) in stimulating cell growth and productivity for a number of cell lines. (genengnews.com)
A variety of cell lines are used for biotherapeutic production. (2bscientific.com)
Lipofectamine 3000 Transfection Reagent delivers a high efficiency for hard-to-transfect cell lines. (thermofisher.com)
When performing transfections in hard-to-transfect cell lines, Lipofectamine 3000 reagent can be an ideal choice. (thermofisher.com)
Lipofectamine 3000 reagent provided higher GFP transfection efficiency than Lipofectamine 2000 and FuGENE HD reagents for all five cell lines. (thermofisher.com)
The Lipofectamine 3000 transfection protocol was developed to be easy to use, while still ensuring optimum performance and reliability in a wide panel of cell lines. (thermofisher.com)
Chinese hamster ovary (CHO) is one of the most widely used non-human mammalian cell lines. (thermofisher.com)
The easy protocol, exceptional plasmid DNA delivery, and the ability to effectively generate stable cell lines makes Lipofectamine 3000 reagent, the perfect reagent for CHO cell transfection experiments. (thermofisher.com)
New South African company NewBiologix has launched with the aim of developing a proprietary and breakthrough platform for the advanced engineering of cell lines used to manufacture gene and cell therapies. (ddw-online.com)
Specifically, the company's technologies will be applied to human (HEK-293) and mammalian (CHO) cell lines. (ddw-online.com)
As a result, biopharma CDMO leaders have gravitated to a platform that efficiently produces robust and stable cell lines. (iptonline.com)
The modifications within the cell line allow for more accurate clone selection when isolating producing cell lines. (iptonline.com)
With stringent regulatory controls in place, what tools can manufacturers utilise to ensure GMP compliance in their cell lines? (iptonline.com)
Cell lines that are fully compliant with GMP standards make them a useful tool for accelerating advanced CLD programmes. (iptonline.com)
Most recombinant proteins are currently produced in prokaryotic cells (mainly the bacterium Escherichia coli ) and a small number of well-characterized mammalian cell lines, such as Chinese hamster ovary (CHO) cells. (frontiersin.org)
Cell banks allow the storage and generation of detailed characterizations of cell lines with specific genomes for research use. (medgadget.com)
Growing importance of cell lines in research: Cell lines are widely used in basic and applied research, and their importance is expected to continue to grow in the coming years. (medgadget.com)
Cell lines are used in a variety of research applications, including drug discovery and development, gene therapy, and tissue engineering. (medgadget.com)
Rising demand for cell lines for producing vaccines to combat the COVID-19 outbreak is boding well for the growth of the mammalian cell banking market. (medgadget.com)
Rational genetic engineering to enhance biotherapeutic proteins has become a reality catalyzed by publication of the genome sequences of multiple Chinese hamster ovary (CHO) cell lines. (bioprocessintl.com)
Analysis of 15 independent G418-resistant CHO lines obtained following fusion suggests the majority contain a complete YAC with moderate amplification in some lines. (ox.ac.uk)
Cytokine defects have also been reported in mammalian cell lines lacking OCRL1 attributed to dysregulation of actin assembly. (medscape.com)
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). (bvsalud.org)

CHO cells have played a significant role in the production of recombinant protein therapeutics since, with CHO cells producing 70% of biologics and monoclonal antibodies in 2016. (wikipedia.org)
Animal cell technology is an area of rapid expansion and one that produces a wide range of high-value products, including vaccines, recombinant proteins, drugs for cardiovascular, respiratory and immune diseases, and monoclonal antibodies. (imperial.ac.uk)

Furthermore, Chinese hamster ovary cells are able to produce proteins with complex glycosylations, post-translational modifications (PTMs) similar to those produced in humans. (wikipedia.org)
More recently, other selection systems have become popular and with vector systems that can more efficiently target active chromatin in CHO cells, antibiotic selection (puromycin) can be used as well to generate recombinant cells expressing proteins at high level. (wikipedia.org)
This sort of system requires no special mutation, so that non-DHFR-deficient host cell culture have been found to produce excellent levels of proteins. (wikipedia.org)
As a result, accumulation of improperly folded proteins is a particularly challenging bottleneck in cell line engineering. (aiche.org)
Host cell proteins (HCPs) are process-related impurities which occur during biopharmaceutical manufacturing. (2bscientific.com)
Protein synthesis inhibition in CHO cells led to rapid death, indicating that CHO cells were pre-disposed to death and that survival proteins needed to be continuously synthesized to protect cells from death. (mit.edu)
Caspases, a class of proteins found to be universally important in inducing apoptosis, were found to be activated in apoptotic CHO cells. (mit.edu)
The absence of any large macromolecules allows for isolation and purification of secreted proteins from the cells. (sigmaaldrich.com)
Membrane trafficking defects caused by mutation in OCRL may explain renal tubular defects observed in Lowe syndrome, including the inability of proximal tubular cells (PTC) to reabsorb low-molecular weight (LMW) proteins and other solutes such as phosphorus and bicarbonate from the glomerular filtrate. (medscape.com)

As the number of gene and cell therapies in the clinic and market continues to grow, improved manufacturing solutions for viral vectors are needed," said co-founder and CEO, Igor Fisch. (ddw-online.com)
Current gene and cell therapies primarily focus on rare diseases. (ddw-online.com)
Herein, we engineered attenuated Salmonella typhimurium VNP20009 with gene circuits to synthetize granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 7 (IL-7) within tumors, which recruited dendritic cells (DCs) and enhanced T cell priming to elicit anti-tumor response. (bvsalud.org)

A majority of CHO cells in culture were found to die by apoptosis, a genetically controlled form of cellular suicide. (mit.edu)
Using these results, a pathway for apoptosis in CHO cells, focusing on the caspases and bcl-2, was suggested. (mit.edu)

CHO cells are the most commonly used mammalian hosts for industrial production of recombinant protein therapeutics. (wikipedia.org)

Many molecular components involved in plant cell wall polymer synthesis have been identified, but it remains largely unknown how these molecular players function together to define the length and decoration pattern of a polysaccharide. (mdpi.com)

IPT: With increasingly sophisticated biologics coming to market, and an emphasis on speed, how is biopharma meeting the cell manufacturing demands of these products? (iptonline.com)

Production du virus influenza dans des cellules HEK-293 cultivées en suspension [Master's thesis, École Polytechnique de Montréal]. (polymtl.ca)
VM), showed that the recovery of motor functions induced implanted either (1) as a solid piece in the lateral ven- by the grafted fetal dopamine neurons was well cor- tricle6 or a cortical cavity8 adjacent to the denervated related with the extent of graft-derived reinnervation caudate-putamen, or (2) as a crude cell suspension of the host caudate-putamen. (lu.se)

Current work is focused on measuring the UPR, product titer, and product quality in a CHO cell line engineered for high productivity of IgG. (aiche.org)
To examine the effect of rTransferrin on CHO cell growth and productivity, two concentrations (1 and 5 µg/mL) of the supplement were tested. (genengnews.com)
5 µg/mL) displayed similar growth and productivity effects in CHO and Sp2/0 cells to iron supplements in common use within the industry, rTransferrin was compared with serum-derived hTf or bTf (each at 5 µg/mL) and three different inorganic iron salts. (genengnews.com)
Our research programme sets out to integrate modelling, experiment design and validation, and control and optimisation into a single framework that would lead to increased productivity, regulated product quality, and reduced costs for mammalian cell culture systems. (imperial.ac.uk)

Some platforms can generate a manufacturing-ready cell line with a comprehensive parental cell line history document. (iptonline.com)

This superior transfection performance allows you to reduce the reagent dose, thus helping lower toxicity risk when transfecting your cell line. (thermofisher.com)
Lipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. (thermofisher.com)
We were very happy and surprised to see Lipofectamine® 3000 reagent provide a more than 10-fold difference in transfection efficiency in our difficult-to-transfect cell line. (thermofisher.com)
Spectacular increase in transfection efficiency and dramatic decrease in cell toxicity. (thermofisher.com)
What cell density do you recommend before transfection in CHO cells? (cellculturedish.com)
Deleterious cell culture conditions can negatively impact not just your next transfection, but all transfections from that point forward. (cellculturedish.com)
Thus, for a CHO cell line with mid log phase at 4×10^6 viable cells/mL, transfecting cells when they reach a density of approximately 1-4×10^6 would be reasonable, with 2-3×10^6 likely being an optimal target density at the time of transfection. (cellculturedish.com)

These experiments proved that early apoptotic cells, which have lost membrane integrity but not chromatin integrity, can be considered dead since they lose the ability to replicate. (mit.edu)

Deletion of ATF4 in parental cancer cells significantly reduces colony formation and metastasis of NDCs, whereas overexpression of ATF4 activates the nonclassical NF-κB signaling pathway to promote chemotherapy-induced metastasis of NDCs. (bvsalud.org)

We isolated the following BA.2 variants in VeroE6/TMPRSS2 cells: hCoV-19/Japan/UT-NCD1288-2N/2022 (NCD1288), hCoV-19/Japan/UT-HP353-1N/2022 (HP353), hCoV-19/Japan/UT-HP354-1N/2022 (HP354), and hCoV-19/Japan/TY40-385/2022 (TY40-385). (nature.com)

In 1957, Theodore T. Puck obtained a female Chinese hamster from Dr. George Yerganian's laboratory at the Boston Cancer Research Foundation and used it to derive the original Chinese hamster ovary (CHO) cell line. (wikipedia.org)
Development of a safe and high-quality Chinese hamster ovary (CHO) cell line is of paramount importance for the chemistry, manufacturing, and controls (CMC) portion of studies that support investigational new drug (IND) applications (1, 2). (bioprocessintl.com)
EX-CELL ® CD CHO FUSION is a chemically defined, animal-component free medium developed for the long-term growth of Chinese Hamster Ovary (CHO) cells. (sigmaaldrich.com)

Subsequently in 1983, CHO cells were mutagenized with gamma radiation to yield a cell line in which both alleles of the DHFR locus were completely eliminated, termed CHO-DG44. (wikipedia.org)

The metabolic dye MTS was added to the remaining media for analysis of viable cell numbers and absorbance was measured from each of the six replicate wells to determine growth. (genengnews.com)

Since the original CHO cell line was described in 1956, many variants of the cell line have been developed for various purposes. (wikipedia.org)

CHO-K1 was mutagenized in the 1970s with ethyl methanesulfonate to generate a cell line lacking dihydrofolate reductase (DHFR) activity, referred to as CHO-DXB11 (also referred to as CHO-DUKX). (wikipedia.org)
Mouse myeloma cell line, Sp2/0 expressing anti TGFb-3, was cultured in DMEM/F12 (SAFC Biosciences), and 2 mM Glutamax™, ITS, 0.5% Probumin. (genengnews.com)
Each cell line produces a different HCP profile, which can vary according to the biopharmaceutical manufacturing process and the nature of the expressed drug. (2bscientific.com)
Commercially-available reagents for HCP monitoring include antibodies raised against individual contaminants, as well as antibodies which have been developed to detect protein fractions derived from a specific cell line. (2bscientific.com)
Increasingly, cell-line development (CLD) is being outsourced to external CDMO partners to help accelerate products to patients. (iptonline.com)
Media and feed have been optimised to support both the initial cell-line engineering process and stable cell-line selection through to large-scale growth and production. (iptonline.com)
The cell-line data are completely validated, and are considered as gold standard by regulators. (iptonline.com)
The main objective of the project is to study the effects of hyperosmotic pressure on the antibody production process in the GS-NS0 cell line, with particular attention to the antibody production mechanism. (imperial.ac.uk)
It is a serine protease ( EC 3.4.21.68 ) found on endothelial cells , the cells that line the blood vessels . (wikidoc.org)
For example, in June 2019 , Selexis SA announced a partnership with Avacta Group Plc for cell line development. (medgadget.com)

It is clear that the biological behaviour of cells is of such complexity that any model, to be robustly predictive, must be sufficiently structured and detailed in terms of the captured biology. (imperial.ac.uk)
Two fundamentals aspects of cell behaviour any model must include are metabolism and the cell cycle. (imperial.ac.uk)

Much of the genetic manipulation done in CHO cells is done in cells lacking DHFR enzyme. (wikipedia.org)

How has cell production developed in the last decade, and what trends are you seeing evolve? (iptonline.com)
Amélioration de la production et de la qualité des protéines thérapeutiques recombinantes produites en culture en mode cuvée-alimentée de cellules de mammifère [Ph.D. thesis, École Polytechnique de Montréal]. (polymtl.ca)
High-Yield Production Process of Influenza Virus-Like Particles in Human Cells Toward Large-Scale Vaccine Manufacturing [Ph.D. thesis, École Polytechnique de Montréal]. (polymtl.ca)
Development of a Production Process for a Virus Like Particle Based Vaccine in Cell Culture [Master's thesis, École Polytechnique de Montréal]. (polymtl.ca)

The complex genetics of CHO cells and the issues concerning clonal derivation of cell population was extensively discussed. (wikipedia.org)

An organoid is a 3D organization of cells that can recapitulate some of the structure and function of native tissue. (mdpi.com)

In 1957, CHO-K1 was generated from a single clone of CHO cells. (wikipedia.org)

According to the latest report published by PMR, the global mammalian cell banking market is projected to account for US$ 1.1 Bn , in terms of value, by 2030 end. (medgadget.com)
The report further projects that the mammalian cell banking market will progress at an average CAGR of 14% through 2030 . (medgadget.com)

To facilitate mammalian genome engineering applications, we provide a web-based software tool to guide the selection and validation of target sequences as well as off-target analyses. (cdc.gov)

Also, CHO cells do not express the epidermal growth factor receptor (EGFR), which makes them ideal in the investigation of various EGFR mutations. (wikipedia.org)

The idea to use transplants of dopa- ment of protocols that allow generation of fully functional mine-producing cells to substitute for the lost midbrain and safe midbrain dopamine neurons from stem cells. (lu.se)

The success of these therapies is driving demand for cells that can be used in research and development of new treatments. (medgadget.com)

Since CHO cells have a very high propensity of genetic instability (like all immortalised cells) one should not assume that the names applied indicate their usefulness for manufacturing purposes. (wikipedia.org)
High temperatures and an altered redox environment may compromise the quality of MAbs produced (e.g., fragmentation, truncation), as can the presence of proteases, reductases, and other chemicals released from dead cells. (bioprocessintl.com)

The spike (S) protein of SARS-CoV-2 mediates viral receptor binding and membrane fusion, both of which are essential for viral infection of host cells. (nature.com)
This vector has been tested by modification of a 550-kb YAC containing part of the human MHC class II region and transfer to CHO cells by protoplast fusion. (ox.ac.uk)

Virtually, engineered VNP20009 prefer to colonize in tumors, and inhibited tumor growth by enhancing DCs and T cell infiltration. (bvsalud.org)
Fibrous papule: a tumor of fibrohistiocytic cells that contain factor XIIIa. (medscape.com)

Plant cells are surrounded by an extracellular matrix that consists mainly of polysaccharides. (mdpi.com)
Cells within the body release extracellular vesicles (EVs) that can contain genetic material and signalling molecules. (dcu.ie)

Cells were then washed in 10 mL Dulbecco's PBS, spun and resuspended in serum-free, growth factor-free medium. (genengnews.com)
With increasing use of technology, rapid industrialization, and more research programs, growing economies such East Asia will experience increasing growth in the mammalian cell banking market. (medgadget.com)
The global mammalian cell banking market is expected to witness robust growth during the forecast period. (medgadget.com)
Various players in the mammalian cell banking market are focusing on growth strategies such as acquisitions and partnerships. (medgadget.com)

Anatomy25

Organisms10

Chemicals and Drugs47

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes55

Information Science5

Yeast4

Study in mammalian cells1

HEK2932

Vitro5

Cytogenicity1

Bacteria2

Assay2

Cultures13

Chromosome3

Therapeutic10

Densities1

Mutagenicity1

Protein production4

Transient Protein Expression1

Biopharmaceutical industry2

HELA3

Processes6

Expression14

Viability2

Lines27

Monoclonal antibodies2

Proteins9

Gene3

Apoptosis2

Therapeutics1

Synthesis1

Biologics1

Suspension2

Productivity4

Platforms1

Transfection7

Chromatin1

Pathway1

20221

Chinese3

Subsequently1

Viable cell1

Variants1

Line10

Behaviour2

DHFR1

Production4

Genetics1

Native1

Clone1

20302

Facilitate1

Epidermal1

Neurons1

Therapies1

High2

FUSION2

Tumor2

Extracellular2

Growth4