Mammalian atp binding cassette transporters. Medical search. Frequent questions

6. Mammalian drug efflux transporters of the ATP binding cassette (ABC) family: an overview. (nih.gov)
18. Blood-brain barrier active efflux transporters: ATP-binding cassette gene family. (nih.gov)
P-glycoprotein (P-gp), an ATP-dependent efflux pump, is associated with the development of multidrug resistance in cancer cells. (nih.gov)
In this study, we report on the presence of efflux transporter activity before oocyte maturation in sea stars and its upregulation after maturation. (nih.gov)
This activity is similar to the multidrug resistance (MDR) activity mediated by ATP binding cassette (ABC) efflux transporters. (nih.gov)
Conventional cancer chemotherapy is seriously limited by tumor cells exhibiting multidrug resistance (MDR), which is caused by changes in the levels or activity of membrane transporters that mediate energy-dependent drug efflux and of proteins that affect drug metabolism and/or drug action. (koreascience.kr)
The results are compatible with the view that substrates for Pgp efflux are driven by the movement of ATP through electrostatic interaction and effective ATP-drug complex formation with net anionic character. (johnshopkins.edu)
MDR1 gene encoding P-glycoprotein is an ATP-dependent drug efflux transporter and related to drug resistance of yolk sac carcinoma. (biomedcentral.com)
The BBB includes a physical hurdle, the metabolic hurdle, and the transportation Rabbit Polyclonal to FPR1 hurdle, including ATP-binding cassette (ABC) efflux transporters (8). (technologybooksindustrialprojectreports.com)
2) The interaction between the main constituents and efflux pumps or intestinal transporters. (frontiersin.org)

ABC transporters often consist of multiple subunits, one or two of which are transmembrane proteins and one or two of which are membrane-associated AAA ATPases. (wikipedia.org)
ABC transporters are considered to be an ABC superfamily based on the similarities of the sequence and organization of their ATP-binding cassette (ABC) domains, even though the integral membrane proteins appear to have evolved independently several times, and thus comprise different protein families. (wikipedia.org)
The third subgroup of ABC proteins do not function as transporters, but are rather involved in translation and DNA repair processes. (wikipedia.org)
Pathogens use siderophores, such as Enterobactin, to scavenge iron that is in complex with high-affinity iron-binding proteins or erythrocytes. (wikipedia.org)
Members of the ABCA subfamily of ABC transporters and other ABC proteins such as ABCB4, ABCG1 and ABCG5/8 implicated in lipid transport play important roles in diverse biological processes such as cell signalling, membrane lipid asymmetry, removal of potentially toxic compounds and metabolites, and apoptosis. (nih.gov)
The importance of these ABC lipid transporters in cell physiology is evident from the finding that mutations in the genes encoding many of these proteins are responsible for severe inherited diseases. (nih.gov)
8] "PEX19 binds multiple peroxisomal membrane proteins, is predominantly cytoplasmic, and is required for peroxisome membrane synthesis. (tcdb.org)
Dietary sterols have recently been shown to passively enter intestinal cells, and, subsequently, the vast majority are pumped back into the gut lumen by ATP-binding cassette (ABC) transporter proteins. (medscape.com)
On the other hand full-length human being ABCA4 stated in mammalian cells was discovered structurally equal to the indigenous proteins from bovine photoreceptors. (careersfromscience.org)
Only 1-2% of the mammalian genome is translated into proteins. (frontiersin.org)
ABC transporters are a family of transporter proteins that mediate drug resistance and low drug bioavailability by pumping various drugs out of cells at the expense of ATP hydrolysis. (koreascience.kr)
We have devised methodologies for HTP cloning in 96-well format to increase the speed of generation of constructs for testing, and miniaturised and streamlined expression platforms for E. coli , insect and mammalian cell cultures for both soluble and membrane proteins. (ox.ac.uk)
As the download Windows 7 Annoyances of times is to public end, defects can bind involved an phosphorylated citron of the IL1R and IL18R leading structures, capping dermatan medicine influencing to endothelial end proteins known by reverse isoforms( TLRs). (evakoch.com)
P-glycoprotein (P-gp), an associate from the ABC transporter proteins family, is usually abundantly distributed around the BBB and acts as an integral transporter which positively effluxes an enormous variety of chemicals over the BBB (9, 10). (technologybooksindustrialprojectreports.com)

IL1B regulates the expression of endometrial genes for prostaglandin synthesis and transport by regulating the endometrial expression of PG-endoperoxide synthase 1 ( PTGS1 ), PTGS2 , AKR1B1 , ATP-binding cassette sub-family C member 4 (ABCC4), ABCC9, solute carrier organic anion transporter family member 2A1 ( SLCO2A1 ), SLCO4C1 , and SLCO5A1 [ 5 , 6 ]. (animbiosci.org)
Pgp is also associated with movement of ATP, an anion, from the cytoplasm to the extracellular space. (johnshopkins.edu)

Most of the uptake systems also have an extracytoplasmic receptor, a solute binding protein. (wikipedia.org)
Transporters are extremely vital in cell survival such that they function as protein systems that counteract any undesirable change occurring in the cell. (wikipedia.org)
Achieving success in the x-ray crystallography of a mammalian membrane protein (MP) is still largely a "shot in the dark", but the probability of success can be improved using brute-force approaches such as conducting crystal trials of several orthologs as opposed to only pursuing the structure of a human MP specifically. (nih.gov)
2] "Nucleotide sequence of the human 70 kDa peroxisomal membrane protein: a member of ATP-binding cassette transporters. (tcdb.org)
7] "Human adrenoleukodystrophy protein and related peroxisomal ABC transporters interact with the peroxisomal assembly protein PEX19p. (tcdb.org)
Experiments using specific and non-specific dyes and inhibitors demonstrated that the increase in transporter activity involves an ABCB protein, P-glycoprotein (P-gp), and an ABCC protein similar to the MDR-associated protein (MRP)-like transporters. (nih.gov)
Western blots using an antibody directed against mammalian P-gp recognized a 45 kDa protein in sea star oocytes that increased in abundance during maturation. (nih.gov)
ABCA4 is an associate from the A subfamily of ATP-binding cassette transporters that includes large essential membrane protein implicated in inherited human being diseases. (careersfromscience.org)
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. (nih.gov)
therefore, this protein appears to contribute to drug resistance in mammalian cells. (nih.gov)
The human ATP-binding cassette superfamily G (White) member 2 ( ABCG2 ) gene and its murine homologue breast cancer resistance protein 1 ( Bcrp1 ) are recently described ATP-binding cassette transporters associated with drug resistance in tumor cell lines, including the MCF-7 cell line, selected for its resistance to mitoxantrone (MCF-7/MitoR). (aacrjournals.org)
HN - 2022 BX - Adolescent Motherhood BX - Teen Mothers BX - Teenage Mothers FX - Pregnancy in Adolescence MH - ADP-Ribosylation Factor 6 UI - D000089982 MN - D8.811.277.40.330.300.400.100.50 MN - D12.776.157.325.515.100.550 MN - D12.776.476.338.400.100.50 MS - A member of GTP-binding protein of the large ras superfamily involved in regulation of membrane trafficking by modulating ENDOCYTOSIS and ACTIN CYTOSKELETON remodeling. (nih.gov)
Therefore, as an alternative, CaMdr1p and Cdr1p, which is an ABC protein (ATP binding cassette) also involved in azole resistance in C. albicans , were independently expressed in a common hypersensitive host JG436 of Saccharomyces cerevisiae . (ias.ac.in)
Lakatos, L. , Klein, M. , Hoefgen, R. & Banfalvi, Z. Potato StubSNF1 interacts with StubGAL83: a plant protein kinase complex with yeast and mammalian counterparts. (mpg.de)
SNP linked with transfer of antioxidant vitamins, in cluding rs2118981 in the cellular retinol binding protein II gene, and that is crucial for vitamin A and retin oid transfer, and rs838892 SCARB1, had been also statistically important predictors while in the ultimate model. (agckinase.com)
Among many genes expressed in the endometrium at the time of implantation, we have shown that the expression of S100A calcium binding protein A7A ( S100A7A ) was differentially expressed in the endometrium on day 12 of pregnancy compared to that on day 12 of the estrous cycle in pigs [ 7 ]. (animbiosci.org)
S100A7A, also known as S100A15, is a member of the S100 protein family and is characterized by two calcium binding sites with a helix-loop-helix (EF-hand) motif [ 8 ]. (animbiosci.org)
A microtubule subunit protein found in large quantities in mammalian brain. (lookformedical.com)

He received his Ph.D. in Molecular Biophysics and Biochemistry at Yale University, and in postdoctoral work at the Scripps Research Institute, he solved the first x-ray crystal structure of any mammalian ATP-Binding Cassette (ABC) transporter called P-glycoprotein which is a cause of multidrug resistance in chemotherapy. (nih.gov)

The ATP-binding cassette transporters (ABC transporters) are a transport system superfamily that is one of the largest and possibly one of the oldest gene families. (wikipedia.org)
ABCA4 is a member of the superfamily of ATP-binding cassette (ABC) transporters implicated in the clearance of retinoids from photoreceptor outer segments and associated with Stargardt macular degeneration. (ubc.ca)

The ATPase subunits utilize the energy of adenosine triphosphate (ATP) binding and hydrolysis to provide the energy needed for the translocation of substrates across membranes, either for uptake or for export of the substrate. (wikipedia.org)
ABC transporters utilize the energy of ATP binding and hydrolysis to transport various substrates across cellular membranes. (wikipedia.org)
The membrane-spanning region of the ABC transporter protects hydrophilic substrates from the lipids of the membrane bilayer thus providing a pathway across the cell membrane. (wikipedia.org)
15. Computer-Aided Recognition of ABC Transporters Substrates and Its Application to the Development of New Drugs for Refractory Epilepsy. (nih.gov)
In view of this result, we asked whether ATP interacts directly with Pgp substrates. (johnshopkins.edu)
We show by measuring the movement of Pgp substrates in electric fields that ATP and drug movement are coupled. (johnshopkins.edu)

Genetic mutations in several ABCA subfamily transporters cause severe-inherited lipid disorders. (ubc.ca)

Likewise, an investigation of common disease variants localized to the Walker-A motif of the nucleotide-binding-domains (NBDs) could help understand how ABCA4 couples binding and hydrolysis of ATP with N-ret-PE transport. (ubc.ca)

2014). Multidrug resistance ATP-binding cassette membrane transporters as targets for improving oropharyngeal candidiasis treatment. (microbeonline.com)

Furthermore, to help us delineate the different molecular mechanisms that underpin STGD1, and to help us understand the transport mechanism of ABC transporters in general, this thesis will characterize disease variants found in the transmembrane and the nucleotide binding domains of ABCA4. (ubc.ca)
An in-depth functional analysis of the transmembrane domains (TMDs) will help us identify residues essential for the binding and translocation of N-ret-PE. (ubc.ca)
Deficiency of ATP8B1 in the hepatocyte may result in loss of asymmetric distribution of the phospholipids in the canalicular membrane, thus reducing membrane stability and function of transmembrane transporters like ABCB11, impairing bile salt transport and decreasing hepatocyte resistance to bile salts, ultimately leading to reduced transport of bile salts in liver and gut resulting in cholestasis and watery diarrhea. (gastrores.org)

5] "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (tcdb.org)
ABCA4 is an ABC transporter encoded by the ABCA4 gene. (ubc.ca)
MDR1 gene, also known as ABCB1 (ATP-binding cassette, sub-family B, member 1) gene, encodes an ATP-dependent drug transporter named permeability glycoprotein (P-glycoprotein). (biomedcentral.com)

1. A curated binary pattern multitarget dataset of focused ATP-binding cassette transporter inhibitors. (nih.gov)
5. C@PA: Computer-Aided Pattern Analysis to Predict Multitarget ABC Transporter Inhibitors. (nih.gov)
7. Identification of novel specific and general inhibitors of the three major human ATP-binding cassette transporters P-gp, BCRP and MRP2 among registered drugs. (nih.gov)
10. Investigational ABC transporter inhibitors. (nih.gov)
Experiments using inhibitors suggest that translation and microtubule function are both required for post-maturation increases in transporter activity. (nih.gov)
These MDR transporters serve protective roles in oocytes and eggs, as demonstrated by sensitization of the oocytes to the maturation inhibitor, vinblastine, by MRP and PGP-specific transporter inhibitors. (nih.gov)
HN - 2016 MH - Adenylyl Cyclase Inhibitors UI - D000067956 MN - D27.505.519.389.108 MS - Compounds that bind to and inhibit the action of ADENYLYL CYCLASES. (nih.gov)
Comparison with other serotonin transporter inhibitors. (eurekaselect.com)

For instance, a potential lethal increase in osmotic strength is counterbalanced by activation of osmosensing ABC transporters that mediate uptake of solutes. (wikipedia.org)

NBD-lipid Uptake Assay for Mammalian Cell Lines. (riken.jp)

Despite the potential clinical application of this finding, the epitopes of the three human P-gp-specific monoclonal antibodies MRK-16, UIC2 and 4E3, which bind to the extracellular loops (ECLs) have not yet been mapped. (nih.gov)
MiRNAs are abundant in many mammalian cell types and as extracellular circulating miRNAs. (micrornalibrary.com)

These ABC transporters preferentially pump plant sterols out of intestinal cells into the gut lumen and out of liver cells into the bile ducts, thereby decreasing sterol absorption. (medscape.com)
It really is presently unclear whether P-gp can be mixed up in BBB transport of AMB, except that two previous studies suggested associations between your rat intestinal P-gp transporter and AMB (13, 14). (technologybooksindustrialprojectreports.com)
We search literatures in scientific databases with key words of "each main SHXXT constituent," in combination with "each main inflammatory pathway target molecule" or each main intestinal transporter, respectively. (frontiersin.org)
We report the effect of five main constituents on target molecules which lies in three main inflammatory signaling pathways, we as well investigate the interaction between constituents and intestinal transporter. (frontiersin.org)

HN - 2016 MH - Adalimumab UI - D000068879 MN - D12.776.124.486.485.114.224.60.250 MN - D12.776.124.790.651.114.224.60.250 MN - D12.776.377.715.548.114.224.200.250 MS - A humanized monoclonal antibody that binds specifically to TNF-ALPHA and blocks its interaction with endogenous TNF RECEPTORS to modulate INFLAMMATION. (nih.gov)

Fluconazole has been shown to be more selective for fungal cytochrome P-450 enzymes than for various mammalian cytochrome P-450 enzyme systems. (mims.com)
One of the important NTSR mechanisms is the enhanced rates of herbicide metabolism (here in after referred to as metabolic resistance) often involving cytochrome P450 monooxygenase (thereafter referred to as P450), ABC transporter, glutathione S-transferase (GST), glycosyltransferase (GT) and peroxidase (POD) [ 9 - 12 ]. (biomedcentral.com)

This integrative network includes receptors and transcription factors, biotransformation enzymes, transporters, antioxidants, and metal- and heat-responsive genes, and is collectively known as the chemical defensome . (springer.com)
Two genes, CYP96A13 and ABCC1 transporter, could play an important role in metabolic resistance to tribenuron-methyl in the resistant flixweed population and justify further functional studies. (biomedcentral.com)
Types 2 and 3 are caused by defect in genes that code for bile acid transporter and a phospholipid translocase, respectively. (gastrores.org)

The term "chemical defensome" refers to a wide range of transcription factors, enzymes, transporters, and antioxidant enzymes that together function to detoxify and eliminate harmful compounds, including xenobiotic and endobiotic chemicals. (springer.com)
Following the purification and cloning of GSTs active in herbicide detoxification in maize in the 1980s [ 9 ], it quickly became apparent that the plant enzymes differed significantly in sequence from their mammalian counterparts [ 10 ]. (biomedcentral.com)

13. Reversal of different drug-resistant phenotypes by an autocatalytic multitarget multiribozyme directed against the transcripts of the ABC transporters MDR1/P-gp, MRP2, and BCRP. (nih.gov)

His research is focused on the various mechanisms by which non-coding RNA transcription controls genome architecture in pluripotent and differentiated mammalian cells. (nih.gov)

In peripheral tissues, the ABC1 transporter (defective in Tangier disease) delivers cholesterol to high-density lipoprotein (HDL) for return to the liver. (medscape.com)
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES . (lookformedical.com)

9] "Characterization and functional analysis of the nucleotide binding fold in human peroxisomal ATP binding cassette transporters. (tcdb.org)

ABC transporters are also involved in multiple drug resistance, and this is how some of them were first identified. (wikipedia.org)
17. The role of ABC transporters in drug resistance, metabolism and toxicity. (nih.gov)
19. Intriguing possibilities and beneficial aspects of transporter-conscious drug design. (nih.gov)
20. In vitro methods in drug transporter interaction assessment. (nih.gov)
Due to severe toxicity of these drugs to insect cells, further characterization of CaMdr1p as a drug transporter could not be done with this system. (ias.ac.in)
Sequencing studies were used to extend this system, and the mammalian GSTs active in drug metabolism are now classified into the alpha, mu and pi classes (Figure 1 ). (biomedcentral.com)
Much focus has been on the reversal of the MDR phenotype by inhibition of ATP-binding cassette (ABC) drug transporters. (koreascience.kr)
The central question of this study is whether drug and ATP transport associated with the expression of Pgp are in any way coupled. (johnshopkins.edu)
We have measured the stoichiometry of transport coupling between drug and ATP release. (johnshopkins.edu)
The drug and ATP transport that is inhibitable by the sulfonylurea compound, glyburide (P. E. Golstein, A. Boom, J. van Geffel, P. Jacobs, B. Masereel, and R. Beauwens, Pfluger's Arch. (johnshopkins.edu)
Mammalian RBCs have no nucleus or DNA drug/toxin targets. (johnshopkins.edu)
From the perspective of drug/ATP complex formation, the RBC serves as an important electrochemical sink for toxins. (johnshopkins.edu)

Bacterial ABC transporters are essential in cell viability, virulence, and pathogenicity. (wikipedia.org)
Instead of ergosterol, the mammalian cell membrane possesses cholesterol. (microbeonline.com)
The sphingomyelin that accumulates in the lysosomes of Niemann-Pick disease-affected cells is thought to arise from the degradation of the cells and their organelles because it is a major component of all mammalian cell membranes. (medscape.com)

This transporter is predominantly expressed in the disc membranes of photoreceptor cells in the retina where it plays a crucial role. (ubc.ca)

May function as a cellular cisplatin transporter. (nih.gov)

Nitric oxide (NO), synthesized from L-arginine by three isoforms of NO synthase (NOS), is a gaseous signaling molecule with an astonishingly wide range of biological and pathophysiological activities, including vasorelaxation, angiogenesis, anti-inflammation, and anti-apoptosis in mammalian cells. (koreascience.kr)

Mammalian cells cannot use plant sterols. (medscape.com)
Genetics of somatic mammalian cells, VII. (riken.jp)
All bind of combined thrombin as they are Abnormal to resolve cells of neurons by which Hippo decapping is recruited or also tagged to antiapoptotic elongating chromosomes. (evakoch.com)

Progressive familial intrahepatic cholestasis or PFIC is a general term used to describe a group of genetic disorders involving the hepatocanalicular transporters. (gastrores.org)

It binds to a specific high affinity receptor ( RECEPTOR, MACROPHAGE COLONY-STIMULATING FACTOR ). (lookformedical.com)

We then identified key amino acids in these regions by replacing mouse residues with homologous human P-gp residues to recover binding of antibodies to the mouse P-gp. (nih.gov)
Chen F, Larsen MB, Sánchez C, Wiborg O. The S-enantiomer of R,S-citalopram, increases inhibitor binding to the human serotonin transporter by an allosteric mechanism. (eurekaselect.com)
Second-generation SSRIs: Human monoamine transporter binding profile of escitalopram and R-fluoxetine. (eurekaselect.com)

14. Rapid detection of ABC transporter interaction: potential utility in pharmacology. (nih.gov)

12. PET and SPECT radiotracers to assess function and expression of ABC transporters in vivo. (nih.gov)
This is the first mammalian ABC transporter shown to function as an importer. (ubc.ca)

Taken together, this information will be useful to develop a full understanding of the mechanism of transport of ABCA4 and similar transporters. (ubc.ca)

GSTs also have non-catalytic roles, binding flavonoid natural products in the cytosol prior to their deposition in the vacuole. (biomedcentral.com)

neutrophil O-glycan cytosol not with CREBP1 and EP300 binds disease of GZMB( granzyme B), which requires endoplasmic for the Many variety of CD8+ particles( Maekawa et al. (evakoch.com)

Dimerization is necessary to form an active transporter. (tcdb.org)

Anatomy7

Organisms7

Diseases3

Chemicals and Drugs70

Analytical, Diagnostic and Therapeutic Techniques and Equipment11

Phenomena and Processes34

Information Science3

Efflux10

Proteins14

Anion2

Protein18

Called P-glycoprotein1

Superfamily2

Substrates6

Subfamily1

Hydrolysis1

Multidrug resistance1

Transmembrane3

Gene3

Inhibitors8

Mediate1

Lipid1

Extracellular2

Intestinal4

Antibody1

Cytochrome2

Genes3

Enzymes2

MDR11

Genome1

Tissues2

Nucleotide1

Drug12

Cell3

Membranes1

Cellular1

Apoptosis1

Cells3

Genetic1

High-affinity1

Human3

Interaction1

Function2

Transport1

Roles1

Cytosol1

Active1