TransfectionMolecular Sequence DataMammalsCell LineBase SequenceAmino Acid SequencePlasmidsPromoter Regions, GeneticDNARNA, MessengerCloning, MolecularCells, CulturedTranscription, GeneticGene Expression RegulationHeLa CellsGenetic VectorsPolyethyleneimineCricetinaeTranscription FactorsDNA, ComplementaryTumor Cells, CulturedDNA-Binding ProteinsGene ExpressionGenes, ReporterMutationLuciferasesSignal TransductionRNA, Small InterferingSequence Homology, Amino AcidRecombinant ProteinsCOS CellsRecombinant Fusion ProteinsGene Transfer TechniquesProtein BindingCell Line, TumorEmbryo, MammalianGreen Fluorescent ProteinsElectroporationCHO CellsBinding SitesNuclear ProteinsBlotting, WesternDNA PrimersCercopithecus aethiopsCarrier ProteinsTOR Serine-Threonine KinasesGenetic TherapyCell NucleusLiposomesChloramphenicol O-AcetyltransferasePhosphorylationReverse Transcriptase Polymerase Chain ReactionCell SurvivalTranscriptional ActivationApoptosisRNA InterferenceMembrane ProteinsFibroblastsSequence AlignmentKineticsProtein Structure, Tertiary3T3 CellsCationsCricetulusProteinsCattleTrans-ActivatorsMicroscopy, FluorescenceLuminescent ProteinsSpecies SpecificityGene Expression Regulation, EnzymologicTime FactorsMutagenesis, Site-DirectedBlotting, NorthernSequence Homology, Nucleic AcidDown-RegulationCell ProliferationProtein-Serine-Threonine KinasesHEK293 CellsModels, BiologicalPolymerase Chain ReactionProtein BiosynthesisCell Line, Transformedbeta-GalactosidaseRabbitsPolylysineRestriction MappingDNA, ViralNIH 3T3 CellsGene Expression Regulation, DevelopmentalEnzyme ActivationProto-Oncogene ProteinsConserved SequencePhenotypeMice, Inbred C57BLRegulatory Sequences, Nucleic AcidRepressor ProteinsCell CycleQuaternary Ammonium CompoundsTransgenes