Mammalian cell line. Medical search. Frequent questions

The results presented in this thesis are the outcome of five different studies aiming to improve and balance the expression levels of recombinant proteins in mammalian cell lines. (kth.se)
The fourth study generated an easy-to-use toolbox for balancing the expression levels of recombinant proteins in mammalian cell lines. (kth.se)
Animal cell technology is an area of rapid expansion and one that produces a wide range of high-value products, including vaccines, recombinant proteins, drugs for cardiovascular, respiratory and immune diseases, and monoclonal antibodies. (imperial.ac.uk)
Focused on mammalian cell lines, our experience covers the development and manufacturing of recombinant proteins such as monoclonal antibodies, fusion proteins, cytokines and enzymes in compliance with international GMP standards (EMA/FDA). (pharmaceutical-business-review.com)
Cell line development is the process of creating stable cell lines for use in a variety of applications, including the creation of biologics (recombinant proteins and monoclonal antibodies), drug screening, and gene functional investigations. (alliedmarketresearch.com)
It is expected research in this area will lead to improved engineering strategies for developing cell lines for higher titers of desired recombinant proteins, including relevant therapeutic protein products. (aiche.org)

Our results lend support to the concept of post-transcriptional RNA operons, but we further present evidence that nuclear proximity of genes may provide substantial functional regulation in mammalian single cells. (nih.gov)
Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. (labettor.com)
P53 is an important transcriptional factor which modulates the expression of more than 130 genes controlled in cell stress-response. (umanitoba.ca)
We aimed to examine the molecular mechanisms underlying the effect of MRV infection on the expression of host genes and the possible role of p53 in the interaction of MRV and host cells. (umanitoba.ca)
The vector construction is based on site-directed integration of genes allowing the production of a reproducible and stable cell line. (pharmaceutical-business-review.com)
These ATG genes are highly conserved in mammalian cells, allowing to study their functions also in higher eukaryotes [ 1 - 4 ]. (hindawi.com)
Here, we used quantitative real-time PCR, profiling the expression of 93 genes in single-cells from three different cell lines. (frontiersin.org)
We found that the total transcript level per cell and the expression of most individual genes correlated with progression through the cell cycle, but not with cell size. (frontiersin.org)
Detailed analysis of cell cycle predictive genes allowed us to define subpopulations with distinct gene expression profiles and to calculate a cell cycle index that illustrates the transition of cells between cell cycle phases. (frontiersin.org)
In conclusion, we provide useful experimental approaches and bioinformatics to identify informative and predictive genes at the single-cell level, which opens up new means to describe and understand cell proliferation and subpopulation dynamics. (frontiersin.org)
Here, we employed single-cell gene expression profiling to describe the dynamic transition between cell proliferative states in three different cell lines using a panel consisting of 93 marker genes. (frontiersin.org)
With roughly 30,000 genes in mammalian genomes, fection with a vector encoding MyoD (Tapscott et al. (lu.se)
explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)
Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)
The fact that the DNA of a fully differentiated (adult) cell could be stimulated to revert to a condition comparable to that of a newly fertilized egg and to repeat the process of embryonic development demonstrates that all the genes in differentiated cells retain their functional capacity, although only a few are active. (who.int)

In contrast to bacteria and green algae, human cells possess a very limited number of 6mA sites which are sporadically distributed across the genome of different cell types. (nature.com)
Our results imply that rare 6mA in the mammalian genome is introduced by RNA m 6 A machinery via a non-targeted mechanism. (nature.com)
Given that the basic genome of a differentiated organism is constant between cells (and we here ignore epigenomics), what mainly discriminates one cell type from another is its expression profile. (biorxiv.org)
The Technology Development group at Life Edit discovers new genome editing systems and establishes new editing capabilities and optimizes them to support efforts developing curative gene editing therapies and gene edited cell therapies. (biospace.com)
We are looking for an enthusiastic, competent, and self-motivated Senior Associate Scientist with a strong background in mammalian cell culture and genome editing to help expand our mammalian cell screening efforts. (biospace.com)
In addition to developing its own pipeline of cell and gene therapies, Life Edit Therapeutics will continue to strengthen its platform of genome-editing enzymes, provide gene-editing expertise to strategic partners, and form other third-party partnerships to discover and develop new therapies. (biospace.com)
To accomplish genome editing in avian species, gene integration into chicken primordial germ cell (PGC) genome using a virus or transposition systems has been widely used, and recently developed programmable genome editing technologies including clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated (Cas9) systems enable to edit the genetic information precisely for maximizing the application potentials of avian species. (bvsalud.org)

By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type. (labettor.com)

The test chemical did not induce chromosome aberrations in the mammalian cell line in the presence and absence of S9 metabolic activation system and hence it is not mutagenic in the chromosome aberration study performed. (europa.eu)

In the final project, the toolbox from the fourth project was employed to develop an in vitro cell-based cancer assay which is a crucial tool in cancer research and drug discovery. (kth.se)
Test chemical did not induce mutation in mammalian cell line in the presence and absence of metabolic activation and hence it is not likely to classify as a gene mutant in vitro. (europa.eu)
The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION. (bvsalud.org)

We analyse two comprehensive transcriptome datasets from human tissues and human-derived cell lines in terms of the expression profiles of the SLC and ABC families of membrane transporters. (biorxiv.org)
The Gini index (coefficient) characterises inequalities of distributions, and is used in a novel way to describe the distribution of the expression of each transporter among the different tissues and cell lines. (biorxiv.org)
Similar trends hold true for the expression profiles of transporters in different cell lines, suggesting that cell lines exhibit largely similar transport behaviour to that of tissues. (biorxiv.org)
By contrast, the Gini coefficients for ABC transporters tend to be larger in cell lines than in tissues, implying that some kind of a selection process has taken place. (biorxiv.org)
The lowest four in tissues are FAM32A, ABCB7, MRPL21 and PCBP1, while the lowest three in cell lines are SF3B2, NXF1 and RBM45. (biorxiv.org)
PCBP1 is both reasonably highly expressed and has a low Gini coefficient in both tissues and cell lines, and is an excellent novel housekeeping gene. (biorxiv.org)
COX-1 is expressed constitutively and is isolated throughout most cell lines in almost all mammalian tissues. (medscape.com)

Mammalian cell lines are often the preferred cell factories for manufacturing biologics since they generate proteins with human-like post-translational modifications, which are often essential features to obtain functional, safe, and effective therapeutics. (kth.se)
In summary, this thesis provides strategies to improve the production process of biologics in mammalian cell lines and thereby contributes to the goal of offering safe, effective, and affordable medicine to patients in every part of this world. (kth.se)
These developments have led to more productive and efficient manufacturing of biologics using mammalian cells. (grandviewresearch.com)

First, we will outline the steps for establishing a tightly regulated gene expression system in human HEK293S cells. (essex.ac.uk)
It has been shown that MRV can manipulate host gene expression and further induce apoptosis and cell cycle arrest in various cell lines. (umanitoba.ca)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)

2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)

In a preliminary study it has been shown that phenolic antioxidants such as methyl 4- O -galloylchlorogenate and 4- O -galloylchlorogenic acid derived from Sapota fruits can induce cytotoxicity in colon cancer cells 23 . (nature.com)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)

We apply a tailored experimental design that eliminates these confounders, and report thousands of intrinsically covarying gene pairs in mouse embryonic stem cells. (nih.gov)
Importantly, it has also been shown that plasmid transfections, specifically in CRISPR experiments, can cause cytotoxicity in embryonic stem cells [ 3 ], which could be of concern to many researchers using this cell type. (idtdna.com)

Genetic Engineering: Utilize molecular biology techniques, such as gene editing (CRISPR/Cas9) and transfection/transduction, to modify cell lines for improved protein expression and desired characteristics. (northreach.uk)
Technical Skills: Proficiency in mammalian cell culture techniques, including CHO/HEK cell line establishment, transfection/transduction, and clone selection. (northreach.uk)
Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. (labettor.com)
A total of six siRNAs were designed and screened through transient transfection assay and knockdown in expression of CP-PVY mRNA was calculated in CHO-k cells. (virosin.org)
Transfection of HEK-Na v 1.3 cells with contactin increases the amplitude of the current threefold without changing the biophysical properties of the channel. (jneurosci.org)
Transfection, selection, and single-cell cloning are all part of the cell line generation process. (alliedmarketresearch.com)
These processes include transient transfection, and the creation of stable pools and clonal cell lines. (lonza.com)
Some cell types cannot tolerate plasmid transfection, so one important consideration is direct cytotoxicity due to plasmid transfection itself. (idtdna.com)
Indeed, research shows that transfecting some cell types with any plasmid may cause cell death [ 1 ], while some transfection reagents (e.g., lipids) used for plasmid transfection are themselves toxic to cells [ 2 ]. (idtdna.com)

This, in turn, is anticipated to contribute to the non-mammalian biopharmaceutical manufacturing segment. (grandviewresearch.com)

Cell Line Development: Design and execute experiments to establish stable and high-producing CHO cell lines suitable for the production of therapeutic proteins or Virus production. (northreach.uk)

This individual will work closely with cross-functional teams, utilizing their expertise in cell biology, molecular biology, and biotechnology to establish robust and high-yielding cell lines. (northreach.uk)
Molecular Biology: use a wide range of techniques for designing and constructing vectors and transfecting mammalian cells. (northreach.uk)
Experience: Minimum of 3 years of industry or academic experience in CHO/HEK cell line development, cell culture, and molecular biology techniques. (northreach.uk)
In-depth models can be utilised as tools in predictive cell biology. (imperial.ac.uk)
It is clear that the biological behaviour of cells is of such complexity that any model, to be robustly predictive, must be sufficiently structured and detailed in terms of the captured biology. (imperial.ac.uk)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)

To provide high-density cell cultures with sufficient iron, elevated concentrations of iron salts are required that utilize low-affinity nontransferrin receptor pathways. (genengnews.com)
At present, it is thought that the efficacy of cell replacement in the CNS would be greatly enhanced if a means to fully reestablish degenerated or disrupted pathways (e.g., nigrostriatal) could be developed. (hindawi.com)
In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)

Single-cell RNA sequencing studies on gene co-expression patterns could yield important regulatory and functional insights, but have so far been limited by the confounding effects of differentiation and cell cycle. (nih.gov)
Assay Development: Design and validate assays to evaluate cell line quality, protein expression levels, and product quality attributes. (northreach.uk)
Experience working with mammalian cell lines is essential, and experience with protein expression systems is highly desirable. (northreach.uk)
A system for stable expression of short interfering RNAs in mammalian cells. (virosin.org)
Mammalian cells are currently the preferred expression system for high-value bio-pharmaceutical products, in particular, monoclonal antibodies. (imperial.ac.uk)
Next, we describe the assembly of this expression system into HEK293S cells and a derivative cell line devoid of complex N-glycosylation. (essex.ac.uk)
However, the detailed molecular mechanisms by which MRV regulates the expression of host cells are largely unknown. (umanitoba.ca)
We found that the expression of Bcl-xL protein in H1299 cell line infected by MRV was majorly controlled by the S1 gene segment which encodes the σ1 cell attachment protein and the σ1s non structural protein, while minorly controlled by L3 gene segment of MRV. (umanitoba.ca)
Contactin/F3, a cell adhesion molecule, has been shown to interact with and enhance surface expression of sodium channels Na v 1.2 and Na v 1.9. (jneurosci.org)
The expression of contactin and Na v 1.3 in the HEK-Na v 1.3 stable cell line significantly increases the current density. (jneurosci.org)
Rentschler's proprietary TurboCell™ mammalian expression platform enables the simultaneous production of up to 20 early-stage drug candidate variants. (pharmaceutical-business-review.com)
Furthermore, the segment is greatly benefited from the introduction of novel and enhanced expression systems, improved process monitoring solutions, cell line engineering tools, automated screening methods, and disposable devices. (grandviewresearch.com)
Recombinant therapeutic protein expression using mammalian platforms can be achieved using a range of workflows optimized for different objectives. (lonza.com)
In this study, we have characterized a novel approach for identification and selection of protein-protein interactions, denoted SPIRE (selection of protein interactions by receptor engagement), which is based on a mammalian expression system. (lu.se)

Mammalian Cell Culture Optimization: Develop and implement cell culture strategies to maximize productivity, viability, and consistency of cell lines. (northreach.uk)
In the first study, we investigated the productivity differences between mammalian cell lines from different origins. (kth.se)
Our research programme sets out to integrate modelling, experiment design and validation, and control and optimisation into a single framework that would lead to increased productivity, regulated product quality, and reduced costs for mammalian cell culture systems. (imperial.ac.uk)
It is a recombinant analog of human transferrin that has shown equivalence to serum-derived human transferrin (hTf) and superiority to bovine transferrin (bTf) in stimulating cell growth and productivity for a number of cell lines. (genengnews.com)
To examine the effect of rTransferrin on CHO cell growth and productivity, two concentrations (1 and 5 µg/mL) of the supplement were tested. (genengnews.com)
5 µg/mL) displayed similar growth and productivity effects in CHO and Sp2/0 cells to iron supplements in common use within the industry, rTransferrin was compared with serum-derived hTf or bTf (each at 5 µg/mL) and three different inorganic iron salts. (genengnews.com)
Current work is focused on measuring the UPR, product titer, and product quality in a CHO cell line engineered for high productivity of IgG. (aiche.org)

The Cell Line Development Scientist will play a key role in the development and optimization of CHO cell lines for the production of therapeutic proteins or virus. (northreach.uk)
Transplantation of immature cells has been considered a potential therapeutic strategy for the damaged adult brain and spinal cord, and there is currently sustained interest in the generation of stem cell lines that could be used to treat certain CNS injuries or disorders. (hindawi.com)

New analytical options include live high-throughput/high-content screening in mammalian cells and computational analysis of live cell data. (cshlpress.com)
To design a high-throughput system with a highly parallel capacity, we utilized the B cell line WEHI-231, as carrier of the decoy receptor. (lu.se)

Specifically, this approach enables the fast supply of material within just six weeks (stable cell pools) or twelve weeks (clonal cell lines) to be used in early drug testing and pre-clinical studies. (pharmaceutical-business-review.com)
Cell lines are usually clonal, meaning that the entire population originated from a single common ancestor cell. (alliedmarketresearch.com)
Transient and stable pool processes are generally used for discovery through early-stage development to enable rapid production and screening of large numbers of protein candidates, while stable clonal cell lines are used for GMP clinical and commercial manufacturing. (lonza.com)
Although many species produce clonal offspring in this fashion, Dolly, the lamb born in 1996 at a research institute in Scotland, was the first asexually produced mammalian clone. (who.int)

Complications arising in these systems include the requirement to grow cultures in complex media, the lack of on-line measurements for many of the key substrates, metabolites, and products, the limited and noisy nature of much of the available experimental data and the extremely complex underlying reaction system. (imperial.ac.uk)
Current understanding of mammalian cell cultures lies in the association of their metabolic behaviour to their antibody production output. (imperial.ac.uk)
To keep the cells in exponential growth, maintain cultures between 3-8x100,000 cells/ml. (sigmaaldrich.com)
Coal samples from mines in Pennsylvania and Utah were placed in contact with cultures of continuous line mouse cells (L-929) and continuous line normal and primary human cells. (cdc.gov)

The TSV extract was transferred into cell culture flasks according to a method previously reported ( 9 ). (cdc.gov)
No CPE was observed in either the Hep-2 or Vero cell line that had been injected with TSV after 7 days of culture at any of the three temperatures tested, and CPE was not found after the fourth passage. (cdc.gov)
Such a weak amplification may have been the result of residual extracellular viruses that remained in the cell culture flask after washing. (cdc.gov)
Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm 2 . (sigmaaldrich.com)
as a result, the current industrial control of hybridoma cell culture is still fundamentally manual. (imperial.ac.uk)
Models of animal cell culture systems have a wide range of potential applications, such as analysis and prediction of experimental results, optimisation of culture conditions for prolonged viability, and perhaps most importantly, the investigation of fundamental metabolic processes and their subsequent elucidation. (imperial.ac.uk)
The integration of these three research tools represents a unique, novel, and interdisciplinary approach to addressing the complicated research and industrial problem of model-based control and optimisation of mammalian cell culture processes. (imperial.ac.uk)
Finally, we describe methods for the growth of these cells lines in scalable suspension culture for the preparation of milligram amounts of recombinant protein. (essex.ac.uk)
Add thawed cells to a conical based centrifuge tube, e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. (sigmaaldrich.com)
Prior to assay set-up, CHO cells were grown to between 70-90% confluence in T75 tissue culture flasks in 10-20 mL sterile culture media, and spun and detached using trypsin. (genengnews.com)
Rentschler employs cutting-edge technologies to ensure safe and stable cell culture and protein purification processes. (pharmaceutical-business-review.com)
Cell line is a general term that applies to a defined population of cells that can be maintained in culture for an extended period of time, retaining stability of certain phenotypes and functions. (alliedmarketresearch.com)
A cell line is a permanently established cell culture that will multiply indefinitely given appropriate fresh medium and space and is frequently used to investigate biological processes in place of primary cells. (alliedmarketresearch.com)
The authors conclude that cell culture techniques are very useful for studying environmental toxicants. (cdc.gov)

a stable cell line. (bio.net)

J Cell Biol (2005) 170 (6): 983-992. (rupress.org)
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). (bvsalud.org)

Examples of essential proteins are antibodies defending our body against foreign invaders and hemoglobulin responsible for importing oxygen to our cells and exporting carbon dioxide out from our cells. (kth.se)
Purified GST fusion proteins of the N and C termini of Na v 1.3 pull down contactin from lysates of transfected HEK 293 cells. (jneurosci.org)
As a result, accumulation of improperly folded proteins is a particularly challenging bottleneck in cell line engineering. (aiche.org)
Membrane trafficking defects caused by mutation in OCRL may explain renal tubular defects observed in Lowe syndrome, including the inability of proximal tubular cells (PTC) to reabsorb low-molecular weight (LMW) proteins and other solutes such as phosphorus and bicarbonate from the glomerular filtrate. (medscape.com)

Cell transformation is induced in mammalian cells. (inchem.org)

The molecular processes behind cell cycle progression have been dissected by numerous morphological studies on live or fixed single cells using a plethora of techniques to visualize components and processes during cell division. (frontiersin.org)

One way to achieve this goal is to engineer mammalian cell factories to improve the quantity and quality of biopharmaceuticals while reducing the production cost. (kth.se)
Non-mammalian cell lines, such as microbial cell lines, are recognized as potent factories. (grandviewresearch.com)

The technique, written into an algorithm, is developed through initial application to a structured model for mammalian cells. (imperial.ac.uk)
By applying the random forests algorithm, a supervised machine learning approach, we show how a multi-gene signature that classifies individual cells into their correct cell cycle phase and cell size can be generated. (frontiersin.org)

Rentschler delivers upstream process (USP) development for cell cultivation and downstream process (DSP) development for protein purification as well as formulation development. (pharmaceutical-business-review.com)

The nucleus of an adult somatic cell (such as a skin cell) is removed and transferred to an enucleated egg, which is then stimulated with electric current or chemicals to activate cell division. (who.int)

In this study we show that contactin coimmunoprecipitates with Na v 1.3 from postnatal day 0 rat brain where this channel is abundant, and from human embryonic kidney (HEK) 293 cells stably transfected with Na v 1.3 (HEK-Na v 1.3). (jneurosci.org)
Traditionally, transferrin has been supplied in the form of serum-derived human transferrin (hTf), bovine transferrin (bTf), or, more recently, with the move toward serum-free medium (SFM), inorganic iron salts have been used to supply iron to mammalian cells. (genengnews.com)
Supplied as a human holo-transferrin analog, rTransferrin binds specifically to the transferrin receptor thereby facilitating the bioavailability of iron to the cell. (genengnews.com)
MiR-483-3p regulates oxaliplatin resistance by targeting FAM171B in human colorectal cancer cells. (nih.gov)
However, it has rarely been considered that human milk may also contain substances bioactive toward host cells. (lu.se)
While investigating the effect of human milk on bacterial adherence to a human lung cancer cell line, we were surprised to discover that the milk killed the cells. (lu.se)
Here, we present a new study on induction of autophagy in human lung epithelial cells by the APD HO53. (researchgate.net)
We used primary acute myeloid leukemia (AML) samples and human AML cell lines to investigate the regulatory mechanisms of autophagy and its role in AML differentiation. (hindawi.com)
Rapidly growing L- cells were the most sensitive and stationary phase primary human cells were the least sensitive to the toxic chemicals in coal. (cdc.gov)
Mammalian cell lines were subjected to extensive safety testing to establish a cell line that is human pathogens free, while maintaining sufficient vaccine yield. (cdc.gov)

Finally, analysis of the mechanism by which multimeric alpha-lactalbumin induces apoptosis in transformed epithelial cells could lead to the design of antitumor agents. (lu.se)

THP-1 cells can also restore the response of purified T lymphocytes to Concanavlin A, show increased CO 2 production on phagocytosis and can be differentiated into macrophage-like cells using for example DMSO. (sigmaaldrich.com)

The global Cell Line and Membrane Market size was valued at $4.5 billion in 2021, and is projected to reach $10.6 billion by 2031, growing at a CAGR of 8.8% from 2022 to 2031. (alliedmarketresearch.com)
The mammalian segment held the highest market share of more than 56.2% in 2022 and CAGR 8.0% owing to the lack of internal expertise in the industry. (grandviewresearch.com)

We further found that the level of Bcl-xL protein, which has been shown to be able to inhibit apoptosis, was increased in H1299 cell lines (both p53-null and p53 positive) infected by T3D, but decreased in the same cell lines infected by T1L. (umanitoba.ca)
Cell cycle analysis suggested activation of apoptosis, without arresting cell cycle progression. (nature.com)
Annexin V-propidium iodide double-staining demonstrated that Sapota fruit extracts potentiate apoptosis rather than necrosis in cancer cells. (nature.com)
When this decoy receptor is engaged by a ligand to the displayed protein/peptide, the receptor expressing cell is rescued from apoptosis. (lu.se)

However, such heterotopic transplants, although capable of alleviating symptoms that benefit from neurotransmitter supplementation, do not re-establish the natural homeostatic regulation of neural activity in the brain and dramatically limit the cell/circuitry replacement strategy to practically only PD. (hindawi.com)
To demonstrate the effectiveness of the CRISPR-mediated transcriptional regulation in chickens, three CRISPRa (VP64, VPR, and p300) and three CRISPRi (dCas9, dCas9-KRAB, and dCas9-KRAB-MeCP2) systems were tested in chicken DF-1 cells. (bvsalud.org)
RNA sequencing analysis of IRF7 CRISPRa and CRISPRi- DF-1 cells revealed the specificity of CRISPRa and CRISPRi-based targeted transcriptional regulation with minimal off-target effects. (bvsalud.org)

Past studies have shown that some fetal nigral cells transplanted in this way can grow a limited number of axonal projections towards the striatum, but most are generally incapable of growing over the distance required to establish functional connections in the striatum in the adult brain [ 8 - 10 ]. (hindawi.com)

Mice possess polyploid trophoblast large cells. (ecologicalsgardens.com)
Lines have been developed using several different strains of mice. (bvsalud.org)

A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
One specific peptide-displaying cell could be identified and amplified, based on a specific receptor engagement, in a background of 12 500 wild-type cells after four selections. (lu.se)

Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)

Subsequently, two ubiquitin-like conjugation systems, ATG5/ATG12 and ATG8, the mammalian homologues of which include LC3, GABARAP, and GATE-16, concert the formation of the double-membraned autophagosome [ 9 ]. (hindawi.com)
1995) and subsequently in primary cells (Heyworth et al. (lu.se)

B ) When pre-formed RNP is introduced by nucleofection into cells, the enzyme rapidly starts cutting targeted genomic DNA. (idtdna.com)

Recent research has shown that pieces of fetal nigral tissue placed in the striatum of 6-OHDA lesioned rats offer greater cell survival and predictability of graft function (in comparison to dissociated nigral cells) in the animal model of Parkinson's disease [ 20 ]. (hindawi.com)
Autophagy is an intracellular degradation system that ensures a dynamic recycling of a variety of building blocks required for self-renewal, homeostasis, and cell survival under stress. (hindawi.com)
Basal macroautophagy (thereafter referred to as autophagy), a catabolic recycling system in cells, is key to maintaining cellular homeostasis and survival. (hindawi.com)
Furthermore, activation of autophagy allows to extend cell survival when exposed to different types of stressors such as starvation or cytotoxic drugs. (hindawi.com)
While the importance of autophagy for cell homeostasis and survival has long been appreciated, its role in tumorigenesis and cancer progression is still developing [ 10 , 11 ]. (hindawi.com)

The main objective of the project is to study the effects of hyperosmotic pressure on the antibody production process in the GS-NS0 cell line, with particular attention to the antibody production mechanism. (imperial.ac.uk)
The Cell Line Development group at Amgen is responsible for manufacturing and optimizing the cell lines utilized in production of Amgen's biologic drug portfolio. (mit.edu)
Moreover, the market is expected to experience growth during the forecast period owing to the rise in development and demand for monoclonal antibodies around the globe, the rise in vaccine production, and technological innovations in cell lines and the membrane. (alliedmarketresearch.com)
In order to measure ER stress in cell lines engineered for high-levels of recombinant protein production, this research aims to demonstrate the presence of ER stress associated with immunoglobulin G (IgG) antibody production, elucidate the progression of UPR in response to highly producing recombinant protein, and determine the effect of UPR on product quality. (aiche.org)

We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)

Somatic-cell nuclear transfer, the technique by which Dolly was created, was first used 40 years ago in research with tadpoles and frogs. (who.int)
if it implants and the pregnancy goes to term, the resulting individual will carry the same nuclear genetic material as the donor of the adult somatic cell. (who.int)

We've screened more than 1,275 cancer cell lines as part of the Cancer Dependency Map (DepMap). (broadinstitute.org)
Here we show that methanolic extracts of Sapota fruit (MESF) induces cytotoxicity in a dose-dependent manner in cancer cell lines. (nature.com)

The lack of continuous marine crustacean cell lines has become an obstacle to conducting research on viral disease in shrimp ( 2 , 3 ). (cdc.gov)

Amitrole induced mutations at two loci in one mammalian cell line. (inchem.org)

Two fundamentals aspects of cell behaviour any model must include are metabolism and the cell cycle. (imperial.ac.uk)
The population balance framework is used to capture cell cycle effects while a single-cell model takes account of some features of intracellular metabolism. (imperial.ac.uk)

During the last 20 years, many researchers searched for substitute cell lines on which to study shrimp viruses ( 4 , 5 ). (cdc.gov)

The biological properties and clinical potential of stem cells elicit that are generated must not be unduly sensitive to small fluctu- continued scientific, commercial, and public interest. (lu.se)

For instance, in April 2020, CEVEC Pharmaceuticals GmbH announced the launch of the ELEVECTA platform, which will produce stable cell lines for AAV (Adeno-associated viral) vector for gene therapy. (alliedmarketresearch.com)

Anatomy41

Organisms23

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Chemicals and Drugs58

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Phenomena and Processes84

Disciplines and Occupations2

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Health Care3

Recombinant proteins6

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Inducible expression1

Chromosome aberration1

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Transfection9

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Human10

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20222

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20201