Chemicals and Drugs
MaleimidesEthylmaleimide
ReagentsThiolReactivityWater-solubleReactsConjugationCrosslinkerHyaluronic AcidGold NanoparticlesAntibodyMaleic anhydrideFunctional groupProteinSynthesisCovalent attachmentProteinsProductsAZDyeSulfhydryl groupsReactionAltersDerivativesDegradationBioconjugationMoleculesVialFluorescentChromatographyAcidSpectraGroupsSpecificChemicalMain
Reagents4
  • Bismaleimides are a class of compounds with two maleimide groups connected by the nitrogen atoms via a linker, and are used as crosslinking reagents in thermoset polymer chemistry. (wikipedia.org)
  • Compounds containing a maleimide group linked with another reactive group, such as an activated N-hydroxysuccinimide ester, are called maleimide heterobifunctional reagents (for example, see SMCC reagent). (wikipedia.org)
  • Due to exceptionally fast reaction rates and significantly high selectivity towards cysteine residues in proteins, a large variety of maleimide heterobifunctional reagents are used for the preparation of targeted therapeutics, assemblies for studying proteins in their biological context, protein-based microarrays, or proteins immobilisation. (wikipedia.org)
  • Several reducing and maleimide reagents were investigated to aid in quantification of total Hcy (tHcy). (cdc.gov)
Thiol13
  • Maleimide-functionalised polymers and liposomes exhibit enhanced ability to adhere to mucosal surfaces (mucoadhesion) due to the reactions with thiol-containing mucins. (wikipedia.org)
  • DABCYL C2 maleimide is an excellent thiol-reactive building block for developing DABCYL-based FRET probes. (anaspec.com)
  • Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. (vectorlabs.com)
  • The maleimide group reacts with free thiol groups on biomolecules and surfaces, such as the hinge region of antibodies after mild reduction of sulfhydryl groups with TCEP or DTT. (vectorlabs.com)
  • ChromaLINK Biotin Maleimide incorporates UV-traceable biotin onto thiol containing proteins, peptides and/or antibodies. (vectorlabs.com)
  • The thiol group to react with HA-Maleimide needs to be in the form of free thiol, and often a reducing reagent agent DTT and preferably TCEP (without the need to remove excessive TCEP) is used to enhance the thiol reactivity. (creativepegworks.com)
  • TCO-PEG6-amido maleimide enables simple and efficient tagging of TCO moiety onto thiol containing antibodies, cysteine-containing peptides and other thiol-containing biomolecules. (axispharm.com)
  • The iFluor® 750 maleimide is reasonably stable and shows good reactivity and selectivity with the thiol group. (aatbio.com)
  • Maleimide NHS Ester (SMCC) is a dual reactive crosslinker that reacts, first, with an amine-containing target and, subsequently, a free thiol at neutral pH. (glenresearch.com)
  • CytoGroup 100nm Maleimide-Activated Gold Nanoparticle Conjugation Kit have been optimized for high efficiency one-step conjugation of thiol-containing ligands to gold nanoparticles. (cytogroup.com)
  • The thiol-organosilica NPs retain fluorescent dye maleimide very well. (nih.gov)
  • In addition, rhodamine B-doped thiol-organosilica NPs show higher fluorescence than thiol-organosilica NPs prepared with rhodamine red maleimide. (nih.gov)
  • The surface of thiol-organosilica NPs contains exposed thiol residues, allowing the covalent attachment of fluorescent dye maleimide and protein maleimide. (nih.gov)
Reactivity1
  • A special feature of the reactivity of maleimides is their susceptibility to additions across the double bond either by Michael additions or via Diels-Alder reactions. (wikipedia.org)
Water-soluble3
  • AZDye™ 568 Maleimide is water-soluble and its fluorescence is pH independent over a wide pH range. (vectorlabs.com)
  • A water soluble, hydrophilic sulfo-Cyanine5 maleimide (Cy5® maleimide analog). (lumiprobe.com)
  • AZDye™ 488 Maleimide is a green-fluorescent, water-soluble dye with pH insensitive emission from pH 4 to pH 10. (clickchemistrytools.com)
Reacts3
  • The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, -SH) at pH 6.5 to 7.5 to form stable thioether bond. (vectorlabs.com)
  • The maleimide functional group reacts selectively with free thiols below pH 7.5. (quantabiodesign.com)
  • However, at pH 7.5 and above, the maleimide double bond reacts competitively with free amines (for example, lysine side chains) and free thiols. (quantabiodesign.com)
Conjugation2
  • This mini kit contains 10 individual reaction vials with lyophilized Maleimide-activated gold nanoparticles for performing ten independent conjugation reactions. (cytogroup.com)
  • This MIDI kit contains 1 vial with lyophilized maleimide-activated gold nanoparticles for performing one independent scale-up conjugation reaction. (cytodiagnostics.com)
Crosslinker4
  • This review discusses Quanta BioDesign's maleimide crosslinker products - what they do and when you might want to use them. (quantabiodesign.com)
  • Our discrete maleimide PEG crosslinker products are consistently our best-selling products. (quantabiodesign.com)
  • If you are already familiar with maleimide chemistry, please read on and discover why we call our maleimide crosslinker products marvelous. (quantabiodesign.com)
  • General structure of Quanta BioDesign's MAL-dPEG ® n -TFP ester maleimide crosslinker products. (quantabiodesign.com)
Hyaluronic Acid1
  • 3. A Rapid Crosslinkable Maleimide-Modified Hyaluronic Acid and Gelatin Hydrogel Delivery System for Regenerative Applications, Gels, 2021, 7(1), 13, Text . (creativepegworks.com)
Gold Nanoparticles1
  • Simply mix your protein with the pre-activated maleimide gold nanoparticles supplied in the kit. (cytogroup.com)
Antibody1
  • For instance, emerging promising targeted drug therapies, antibody-drug conjugates, are constituted of three main components: a monoclonal antibody, a cytotoxic drug, and a linker molecule often containing a maleimide group, which binds the drug and the antibody. (wikipedia.org)
Maleic anhydride3
  • Maleimide and its derivatives are prepared from maleic anhydride by treatment with amines followed by dehydration. (wikipedia.org)
  • Styrene-Maleimide/Maleic Anhydride Alternating Copolymers: Recent Advances and Future Perspectives. (bvsalud.org)
  • Alternating sequencing of styrene -maleimide/ maleic anhydride (S-MI/MA) in the copolymer chain is known for a long time . (bvsalud.org)
Functional group1
  • The extended PEG3 linker preserves biotin/streptavidin affinity and maintains protein solubility after modification while the maleimide functional group (d), efficiently modifies thiols in aqueous buffers. (vectorlabs.com)
Protein2
  • We recommend this product for protein labeling, including labeling of antibodies as a perfect replacement for Cy5® maleimide. (lumiprobe.com)
  • If DTT is used you must remove free DTT by dialysis or gel filtration before conjugating a dye maleimide to your protein. (aatbio.com)
Synthesis1
  • HA-MAL derivatives with lower or higher maleimide substitution ratio (e.g. 1% and 10%) may be offered through custom synthesis service. (creativepegworks.com)
Covalent attachment1
  • Pierce Maleimide Activated Plates provide covalent attachment (plate capture) of sulfhydryl-containing molecules, such as cysteine-terminated peptides. (fishersci.com)
Proteins2
  • Maleimides linked to polyethylene glycol chains are often used as flexible linking molecules to attach proteins to surfaces. (wikipedia.org)
  • IRDye 680LT Maleimide is used to label molecules with free sulfhydryl groups, such as cysteine residues in proteins, at physiological pH. (licor.com)
Products1
  • Maleimides (transformation products of chlorophylls and bacteriochlorophylls) were studied in recent sediments from the Swiss lake Rotsee and the Romanian Black Sea Shelf to investigate chlorophyll degradation, the role of oxygen inmaleimide formation, and to identify their sources. (edu.au)
AZDye1
  • AZDye™ 568 Maleimide is a bright, and highly photostable, orange-fluorescent probe optimally excited by the 568 nm laser line on the Ar-Kr mixed-gas laser. (vectorlabs.com)
Sulfhydryl groups1
  • Use IRDye 750 Maleimide to label free sulfhydryl groups for flow cytometry, microscopy, or other applications. (licor.com)
Reaction2
  • If you are not familiar with maleimide chemistry, we encourage you to visit our page on maleimide reaction chemistry to familiarize yourself with how maleimides work. (quantabiodesign.com)
  • The dynamics of the ring-closure reaction of three different bis(thiophen-3-yl)maleimides are investigated using ultrafast spectroscopy in the visible range. (cipsm.de)
Alters1
  • Leaving the acid end unmodified after conjugating the maleimide moiety alters the conjugate's surface charge. (quantabiodesign.com)
Derivatives2
  • Maleimides also describes a class of derivatives of the parent maleimide where the NH group is replaced with alkyl or aryl groups such as a methyl or phenyl, respectively. (wikipedia.org)
  • Maleimides (1H-Pyrrole-2,5-diones) are monopyrrolic pigment derivatives with specific alkyl side chains that can be directly linked to their tetrapyrrole precursors, most notably chlorophylls and bacteriochlorophylls. (edu.au)
Degradation1
  • Novel maleimide degradation indices are proposed to estimate the degree ofOMdegradation (OM freshness/degradability). (edu.au)
Bioconjugation1
  • Maleimide-mediated methodologies are among the most used in bioconjugation. (wikipedia.org)
Molecules3
  • Mono- and bismaleimide-based polymers are used for high temperature applications up to 250 °C (480 °F). Maleimides linked to rubber chains are often used as flexible linking molecules to reinforce rubber in tires. (wikipedia.org)
  • The structures of the molecules differ with respect to substitution of the thiophene ring and the maleimide. (cipsm.de)
  • Thermo Scientific™ Pierce Maleimide Activated Plates are ideal for binding sulfhydryl-containing molecules that are difficult to coat onto polystyrene plates, such as peptides that contain a terminal cysteine. (fishersci.com)
Vial1
  • Add anhydrous DMSO into the vial of iFluor™ 750 maleimide to make a 10 mM stock solution. (aatbio.com)
Fluorescent1
  • Maleimide Activated Plates are available in clear for colorimetric assays, white for chemiluminescent assays and black for fluorescent assays. (fishersci.com)
Chromatography1
  • A new method for the rapid analysis of 1H-Pyrrole-2,5-diones (maleimides) in environmental samples by two-dimensional gas chromatography time-of-flight mass spectrometry. (edu.au)
Acid1
  • Naturally occurring maleimides (i.e. "free" maleimides) and maleimides obtained after chromic acid oxidationof sediment extracts (i.e. "bound" maleimides) were analysed. (edu.au)
Spectra1
  • Superimposed spectra of BSA biotinylated using ChromaLINK Biotin Maleimide. (vectorlabs.com)
Groups3
  • Hyaluronate Maleimide (HA-MAL) is a HA derivative with reactive maleimide groups. (creativepegworks.com)
  • The maleimide groups are conjugated to HA through an amide bond. (creativepegworks.com)
  • An analog without sulfo groups, Cyanine5 maleimide , is also available. (lumiprobe.com)
Specific1
  • Some other minor maleimides with specific alkylation pattern also originate from bacteriochlorophylls, while the source of others could not be identified. (edu.au)
Chemical1
  • Maleimide is a chemical compound with the formula H2C2(CO)2NH (see diagram). (wikipedia.org)
Main1
  • Tetrapyrrolic pigments related to chlorophylls were the main source of maleimides, although variable contributions of other sources such as cytochromes and/or phycobilins cannot be completely ruled out.The predominantMe,Et maleimide and Me,vinylmaleimide most likely originate mainly from chlorophyll a related pigments. (edu.au)