AnatomyDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation ScienceHealth Care
MicrodissectionLaser Capture MicrodissectionLasersDissectionHistocytological Preparation TechniquesSperm RetrievalGene Expression ProfilingOligonucleotide Array Sequence AnalysisReverse Transcriptase Polymerase Chain ReactionMicromanipulationParaffin EmbeddingPolymerase Chain ReactionRNA, MessengerImmunohistochemistryTissue FixationCryoultramicrotomyTissue EmbeddingHematoxylinRNANucleic Acid Amplification TechniquesLoss of HeterozygosityLaser TherapyAzoospermiaDNA, NeoplasmChromosome PaintingIn Situ Hybridization, FluorescenceIn Situ HybridizationFixativesProteomicsFrozen SectionsPyronineRNA, NeoplasmGene Expression Regulation, NeoplasticFormaldehydeGene ExpressionBronchiolesMicrosatellite RepeatsEpithelial CellsMicroarray AnalysisStaining and LabelingBiopsyMicrosurgeryOrotate PhosphoribosyltransferaseGene AmplificationBase SequenceChromosome BandingMicrotomyReproducibility of ResultsStromal CellsGene Expression RegulationComputer SystemsMolecular Sequence DataCell SeparationTestis
LaserTranscript abundanceOcularGenomicsTissueMRNAVendorsChromosomesTechniquesTumorProteinAnalysesAnalysisCell typesEstablishDevelopmentMarketHumanChallengeTreatmentDataPresenceLaserIntratumoralNeuronsMolecularColorectal cancerCytokineFindingsMethodsGenesPregnantDetectionCellSystemContributionsStudyFoundRegionIncludeResponseResearchDisease
Laser21
  • Histology Tissue microarray Laser capture microdissection Powers, Thomas W. (wikipedia.org)
  • The laser capture microdissection market is witnessing growth in the academic and research centers segment due to the increased funds allocated by government agencies to promote research in fields such as neurology, oncology, and proteomics. (technavio.com)
  • The laser capture microdissection market will continue to grow in the Americas throughout the predicted period. (technavio.com)
  • Dr. Lubman's team used a combination of immunostaining with laser capture microdissection (immuno-LCM). (genengnews.com)
  • To run, harm the Print shop Laser Capture in Microscopy and Microdissection. (green-frontier.de)
  • depend the shop Laser Capture in Microscopy and Microdissection, disclaim the year within the contacted middle and frames67 polyoxyethylene. (green-frontier.de)
  • complete your shop Laser Capture in Microscopy and Microdissection so role ahead can search it below. (green-frontier.de)
  • The such Many concentrations after a shop Laser Capture in Microscopy and Microdissection 2002 can make normal, and run you with low animals and employees. (green-frontier.de)
  • What we don't know however - and what I am studying using techniques such as laser capture microdissection, RNA-ISH and In Situ PCR - are the molecular mechanisms underlying the regulation of this evolution. (bccrc.ca)
  • Peking) roots infected by incompatible (I) and compatible (C) populations of soybean cyst nematode [SCN] (Heterodera glycines) were collected using laser capture microdissection. (usda.gov)
  • Neurone, astrocyte, and endothelial cell-enriched mRNA, obtained by immuno-laser capture microdissection of temporal cortex (Brodmann area 21/22) from 6 cases with self-reported T2D in the Cognitive Function and Ageing Study neuropathology cohort, and an equal number of age and sex-matched controls, was assessed by microarray analysis. (biomedcentral.com)
  • We then developed a process to isolate single CTCs using laser capture microdissection for genome sequencing. (ubc.ca)
  • Spectra-Physics' new laser has been demonstrated to enable major advances in the fast, precision manufacturing of many types of microelectronic devices and components. (laserfocusworld.com)
  • Analysis of Genome-Wide Monoallelic Expression Patterns in Three Major Cell Types of Mouse Visual Cortex Using Laser Capture Microdissection. (welgene.com.tw)
  • White matter perivascular and intrameningeal immune infiltrates, including B cell follicle-like structures, were isolated from brain sections using laser capture microdissection. (biomedcentral.com)
  • Dr. Anisimova has been a pioneer in the development of NPDS and explained that by implementing use of the laser for dissecting the scleral flap, CLASS overcomes the major challenge of NPDS, which is the risk of inadvertent trabeculo-Descemet's membrane perforation. (ophthalmologytimes.com)
  • Dans ce projet, nous avons analysé l'expression de nombreux gènes (gènes structuraux et gènes de défense) par PCR quantitative en temps réel, au sein des différentes couches épidermiques isolées d'explant de peau humaine saine par microdissection laser (Laser Capture Microdissection, LCM). (hal.science)
  • In the present work, we have studied the expression of a number of genes (antimicrobial peptide genes and pro-inflammatory cytokine genes) in different layers of healthy human epidermis using quantitative real-time PCR coupled to Laser Capture Microdissection (LCM). (hal.science)
  • This co-culture method simplified observation/monitoring of the two cell types and was particularly suited for laser microdissection-based retrieval of the desired cells for downstream gene expressions studies. (elsevierpure.com)
  • Furthermore, by merging live-cell laser beam microdissection with solitary cell transcriptomics, we set up that induction of interferon activated gene expression happens in micronucleated cells. (biopaqc.com)
  • His work with Arcturus Applied Genomics on the genomics of human breast cancer using laser capture microdissection led to the development of a tumor marker database and biorepository. (aacc.org)
Transcript abundance1
  • However, compared to typical transcriptomics studies, dual-species transcriptomics studies are technically challenging due to a difference in the proportion of reads from the major and minor organisms in the system, where major and minor refer to transcript abundance. (biomedcentral.com)
Ocular2
  • You will learn retinal anatomy and the techniques of ocular microdissection, cryosectioning, plastic sectioning and fluorescence microscopy. (upenn.edu)
  • Expression of DCT was absent or barely detected in retina tissues, various human ocular cells, and major organs. (molvis.org)
Genomics1
  • Z.N. Demou, 'Time-Lapse Analysis and Microdissection of Living 3D Melanoma Cell Cultures for Genomics and Proteomics,' Biotechnology and Bioengineering, vol. 101, no. 2, pp. 307-316, October 2008. (epfl.ch)
Tissue2
  • Tissue corresponding to the dentate gyrus (DG) and regions CA1, CA2, and CA3 of Ammon's horn was removed by microdissection from transverse hippocampal slices and was used to establish cultures of dissociated cells. (johnshopkins.edu)
  • Gut-associated lymphoid tissue is a major target and reservoir of human immunodeficiency virus (HIV)-infected T-cells. (elsevierpure.com)
MRNA1
  • Toll-like receptor (TLR) -4 mRNA expression is higher in the acute stage of major depressive disorder (MDD) compared with healthy controls. (jneuropsychiatry.org)
Vendors3
Chromosomes2
  • With the human DNA probe, signals were detected in the major sites of the ribosomal genes on chromosomes 5I (7H) and 6I (6H) only when the chromosome preparations were obtained using an optimized technique with obligatory pepsin treatment followed by hybridization. (centrocardiobogota.com)
  • Based on these microdissections we anchored all linkage groups to the five chromosomes of N. vitripennis. (elsevierpure.com)
Techniques1
  • The combination of single-cell RNA sequencing and microdissection techniques that preserves positional information has become a major tool for spatial transcriptome analyses. (authorea.com)
Tumor2
  • Not surprisingly, the major groups of differentially expressed proteins were involved in tumor migration, invasion, and progression. (genengnews.com)
  • A major contribution to laboratory medicine was made when Dr. Wittliff, working with NEN/DuPont, developed the first FDA-approved kits for assessing steroid receptor concentrations in tumor biopsies. (aacc.org)
Protein1
  • The application of these new proteomic technologies to study cellular activities, will lead to a faster sample throughput and increased sensitivity for the detection of individual proteins, thus providing major opportunities for the discovery of new biomarkers for the early detection of protein alterations associated with the progression of the disease state. (usuhs.edu)
Analyses1
Analysis3
  • Research analysis on the global microdissection market identifies that the growing adoption of next-generation sequencing (NGS) will be one of the major factors that will have a positive impact on the growth of the market. (technavio.com)
  • The rising demand for efficient forensic tools is motivating researchers to incorporate microdissection tools during analysis. (technavio.com)
  • What are the key outcomes of the five forces analysis of the global microdissection market? (technavio.com)
Cell types1
  • However, usefulness of microdissection is often limited by the difficulties in identifying desired cell types by morphology alone. (genengnews.com)
Establish1
  • We were the first major medical center to establish a fully integrated pathology department combining the various arms of testing-anatomical, clinical, molecular/genetic, and cytological. (pathologyinformatics.org)
Development2
  • This paper summarizes a major milestone in the continuous development of NanoVelcro assays pioneered by our research group," Tseng said. (sciencedaily.com)
  • Objective: Tobacco smoke exposure is a major risk factor for aortic aneurysm development. (elsevierpure.com)
Market4
Human1
  • The major findings are: 1) We have developed an optimized LCM protocol for the isolation of human epidermal layers. (hal.science)
Challenge1
  • Such computerized extraction of quantitative information out of the rapidly expanding amount of acquired data remains a major challenge. (epfl.ch)
Treatment2
  • This intratumoral heterogeneity, and resulting diversity in clinically relevant traits, presents a major barrier to treatment and a mechanism for progression across cancer types. (bccrc.ca)
  • Being able to predict, and then timely track the response to treatment is a major issue that affects survival and costs! (pharmaceuticalintelligence.com)
Data1
  • The sequencing data confirmed the presence of major driver mutations, including PTEN and TP53, as well as heterogeneous characteristics of individual CTCs. (ubc.ca)
Presence1
  • In part, the of major areas is addressed in survey by certain presence, percent and strength. (yvonne-unden.de)
Laser17
  • The aim of this study was to identify intratumoral heterogeneity in CRC by using laser microdissection (LMD) in comparison to the routinely used method. (nih.gov)
  • Histology Tissue microarray Laser capture microdissection Powers, Thomas W. (wikipedia.org)
  • Tissues from these lesions are microdissected using laser capture microdissection and analyzed for LOH and other molecular abnormalities. (nih.gov)
  • Here we isolated neuromelanin-positive dopamine neurons by laser capture microdissection from post-mortem human substantia nigra samples recovered at both early and advanced stages of PD. (frontiersin.org)
  • Analysis of Ostýý and Ostýý mRNA levels in mouse Purkinje and hippocampal cells isolated via laser capture microdissection supported these findings. (nih.gov)
  • The aim of this study was to define the major transcriptional programmes involved in fibrogenesis in IPF by profiling unmanipulated myofibroblasts within fibrotic foci in situ by laser capture microdissection. (bmj.com)
  • 13. High frequency of human herpesvirus 6 DNA in multiple sclerosis plaques isolated by laser microdissection. (nih.gov)
  • Laser capture microdissection revealed that adenocarcinoma and squamous carcinoma components of ASCP harbor similar genomic variations, indicating that the origin of tumor components is the same or similar. (nih.gov)
  • Strong upregulation of PDE1C in pulmonary arterial vessels in the idiopathic PAH lungs compared with healthy donor lungs was noted on the mRNA level by laser-assisted vessel microdissection and on the protein level by immunohistochemistry. (nih.gov)
  • We had the inventors and developers of pharmacokinetics, and laser-capture microdissection. (nih.gov)
  • In this study, we used laser capture microdissection to isolate hippocampal CA1 neurons and determined their pre-clinical transcriptional response during infection. (prolekarniky.cz)
  • To identify the cellular sources of the expressed genes, cells from various layers of the retina were obtained using laser capture microdissection (LCM), and their mRNAs were isolated. (elsevierpure.com)
  • The approaches showcased would include fluorescence, confocal and super resolution fluorescence imaging, laser capture microdissection microscopy and atomic force microscopy. (indiabioscience.org)
  • Through laser capture microdissection, I will be able to isolate sinus nodes and sequence the RNA of pacemaker cells from these mice. (berkeley.edu)
  • Immunohistochemistry and laser microdissection of patient renal biopsies will be used to assess in situ protein and RNA expression of innate interferons and their gene signatures. (rochester.edu)
  • The Histopathology Core has extensive experience and standardized methodologies, and also incorporates laser capture microdissection, Real-Time PCR analysis, and tissue arrays. (nih.gov)
  • Dr. Belmonte's group uses cutting edge next generation molecular and plant laser microdissection techniques to improve crop production and protection of some of Canada's most important agricultural crops. (umanitoba.ca)
Intratumoral2
  • Assessment of intratumoral heterogeneity requires cell-by-cell or microdissection methods that do not assume a tumor is a uniform mass of identical cells. (medscape.com)
  • [ 24 ] Although the latter clearly show that even within major breast cancer subtypes like luminal there are clinically important subsets that modify patient outcome and necessitate unique therapeutic approaches, they do not address intratumoral cell heterogeneity. (medscape.com)
Neurons2
  • Major symptoms in Parkinson's disease (PD) are caused by the degeneration of midbrain dopamine (mDA) neurons within the substantia nigra. (frontiersin.org)
  • Analysis of differential gene expression and gene co-expression relationships demonstrates that brain-wide variation strongly reflects the distributions of major cell classes such as neurons, oligodendrocytes, astrocytes and microglia. (nature.com)
Molecular1
  • One of the major advantages of FNA is that it is less invasive and better tolerated than a larger tissue biopsy and in many clinical scenarios provides sufficient material not only for morphological evaluation but also for ancillary studies including molecular testing. (cap.org)
Colorectal cancer1
  • Objective Distant metastasis is the major cause of cancer-related death in patients with colorectal cancer (CRC). (bmj.com)
Cytokine1
  • Conclusions: Retinal glial cells, including astrocytes and Müller cells, are a major source of cytokine induction after RD. The increased expression and release of MCP-1 may be an important cause of photoreceptor degeneration associated with RD. This study helps to understand the mechanisms of RD-induced photoreceptor degeneration. (elsevierpure.com)
Findings1
  • Subsequent to these findings, a major effort was made to disseminate this information to eye care specialists and the general public. (nih.gov)
Methods1
  • However, there are different methods for obtaining sperm from the male, including microdissection testicular sperm extraction (mTESE) and multiple needle-pass percutaneous testicular sperm aspiration (TESA). (centrial.org)
Genes2
  • We found that a major cluster of genes is altered during pre-clinical disease after which expression either returns to basal levels, or alternatively undergoes a direct reversal during clinical disease. (prolekarniky.cz)
  • His laboratory participated in disentangling the natural history of the 16p11.2 rearrangements, i.e. their evolution, associated phenotypes and identification of major driver genes. (agbt.org)
Pregnant1
  • His book, "How to Get Pregnant", published by Little, Brown and Company in 2007, is a completely revised and updated edition of his classic series of "How to Get Pregnant" books, which have been major bestsellers. (infertile.com)
Detection1
  • The major disadvantage is its detection threshold requiring at least a 10 % mutant allele fraction, or at least a 20 % malignant cell fraction. (biomedcentral.com)
Cell2
  • The calcium/calmodulin-dependent phosphodiesterase 1 (PDE1) may play a major role in vascular smooth muscle cell proliferation. (nih.gov)
  • Isolation of Fetal Cell from Maternal Blood by Glycophorin A[GPA] Staining and Microdissection. (ogscience.org)
System1
Contributions3
  • MALDI-MSI is providing major contributions to the understanding of diseases, improving diagnostics, and drug delivery. (wikipedia.org)
  • NAE membership is bestowed on those who have made crucial contributions to new and developing fields of technology and major advancements in traditional fields of engineering. (nih.gov)
  • Current NIBIB Director Bruce Tromberg said, "Peter's discoveries have had enormous impact on the development of MRI and his generous contributions over the years have played a major role in the growth of NIBIB and the biomedical imaging and engineering community around the country. (nih.gov)
Study1
  • The major objective of our study was to compare the scab nonhost resistance in apple and in European pear, at the phenotypic and transcriptomic levels. (peercommunityjournal.org)
Found1
  • From these results, three major criteria were found to determine CdpNPT's affinity for an alkyl donor: carbocation stability, steric hindrance, and hydrophobic interaction within the active site. (curiosity2creativity.net)
Region1
  • Forty of 72 (55%) PIOL patients expressed the bcl-2 t(14;18) translocation at the major breakpoint region. (arvojournals.org)
Include1
  • In particular during early stages of disease, motor functions are affected and major symptoms include bradykinesia, rigidity, tremor and postural instability. (frontiersin.org)
Response1
  • To screen for expressional changes in response to RD, major candidates for cytokines, chemokines, and growth factors were broadly examined by quantitative real time polymerase chain reaction (QPCR). (elsevierpure.com)
Research2
  • As such, CTRC is a major resource for those wishing to diversify and explore new avenues of research by developing cutting-edge technologies and project-specific applications. (nih.gov)
  • The workshop is designed for PhD students, postdoctoral fellows and researchers at similar stages of their career who intend to use imaging as a major tool in their imminent research. (indiabioscience.org)
Disease1
  • This has been a major advance in the field of immunologic disease with broad implications in rheumatoid arthritis, lupus, diabetes, malignancy, and immune deficiency. (rochester.edu)