Mainly catalytic domain. Medical search. Frequent questions

Each subunit has a region called a motor domain (also known as its 'head') that can bind to the microtubule and to a molecule called ATP, which provides the energy required for the motor to step forward. (elifesciences.org)
The PKA Catalytic Subunit can itself be inactivated by phosphorylation. (exploreinnerspace.org)
The subunit of the homo-octameric enzyme folds into a two-domain structure characteristic of a metal dependent amidohydrolase, with a twisted TIM (triosephosphateisomerase)-barrel and a smaller β-sandwich domain. (scilifelab.se)
Protein phosphatase 2A (PP2A) is an enzymatic complex containing three subunits: a catalytic subunit, a scaffolding subunit, and one of several regulatory subunits responsible for dictating substrate specificity of the enzyme (42). (grandlacs-med-journal.com)
Orthologous to human PIK3C2A (phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha). (nih.gov)

Dissociated PKA catalytic subunits are free to phosphorylate and activate/deactivate other proteins. (exploreinnerspace.org)
PF cells were found to express regulatory (p85) and catalytic (p110α and p110β) subunits of phosphatidylinositol 3′-kinase (PI3′-kinase). (jneurosci.org)

Here we present a novel activity of recombinant ADAM12-S and its domain deletion mutants on S-carboxymethylated transferrin (Cm-Tf). (ox.ac.uk)
Negative stain TEM and image averaging showed the triangularity of a recombinant CESA cytosolic domain, consistent with previous modeling of its trimeric nature from small angle scattering (SAXS) data. (osti.gov)

Here, we addressed the allosteric regulation of FAK by performing all-atom molecular-dynamics simulations of a FAK fragment containing the catalytic and FERM domains, and comparing the dynamics in the absence or presence of ATP and PIP₂. (rcsb.org)
It has been found that human PTGS2 (COX-2) functions as a conformational heterodimer having a catalytic monomer (E-cat) and an allosteric monomer (E-allo). (wikipedia.org)

We identified a network of mainly charged residue-residue interactions spanning from the PIP₂ binding site to the distant interface between the kinase and FERM domains, comprising candidate residues for mutagenesis to validate the predicted mechanism of FAK activation. (rcsb.org)
The enzyme consists of the wild type catalytic domain of human MMP-12 (residues 106-263, UniProtKB accession P39900). (giottobiotech.com)
The catalytic domain contains two zinc ions and at least one calcium ion coordinated to various residues. (wikipedia.org)
the three histidine residues that coordinate with the catalytic zinc are conserved among all the MMPs. (wikipedia.org)
The catalytic core domain contains three highly conserved residues Asp64, Asp116 and Glu152 (the DDE motif) that are essential for the catalytic activity of IN. (biomedcentral.com)
The enzyme consists of residues Phe 107 -Pro 449 (NM_004994), which comprises the catalytic/fibronectin domain of human MMP-9, with a C-terminal purification tag. (enzolifesciences.com)
Inhibition of the kinase domain is halted through binding of the Ras-binding domain, or residues 155-227 of the protein, to the Ras-GTP effector domain. (news-medical.net)

The heavy chain is responsible for both receptor some species of the genus Clostridium, in particular, Clostridium binding via its C-terminal (CT) binding domain [4,5] (HC) and botulinum, C. butyricum, C. baratii, and C. argentinense. (cdc.gov)

ADAM12 exists as two splice variants: a classical type 1 membrane-anchored form (ADAM12-L) and a secreted splice variant (ADAM12-S) consisting of pro, catalytic, disintegrin, cysteine-rich, and EGF domains. (ox.ac.uk)

The epidermal growth factor domain has a calcium ion - binding site that to some degree mediates interaction with the tissue factor exposed at the site of vessel injury. (medscape.com)

One is in the activation segment of the kinase domain. (news-medical.net)
The other hotspot is the glycine -rich loop of the kinase domain. (news-medical.net)
Conserved region 3 (CR3) is a catalytic protein kinase domain. (news-medical.net)
CR1 inhibits the activity of CR3, the kinase domain, regulating the overall signaling activity of the molecule. (news-medical.net)
In order to become active, the CR3 kinase domain must be converted to its active form by phosphorylation of the activation loop. (news-medical.net)
Eleven novel JAK3 mutations in patients with severe combined immunodeficiency-including the first patients with mutations in the kinase domain. (lu.se)

The catalytic domain is homologous to mammalian peroxidases such as myeloperoxidase. (wikipedia.org)

Conserved region 1 (CR1) is a Ras-GTP-binding self-regulatory domain. (news-medical.net)

The catalytic domain of MMP-3 can be inhibited by tissue inhibitors of metalloproteinases (TIMPs). (wikipedia.org)
The hemopexin-like domain has been shown to play a functional role in substrate binding and/or in interactions with the tissue inhibitors of metalloproteinases (TIMPs), a family of specific MMP protein inhibitors. (wikipedia.org)
Among the tissue inhibitors of metalloproteinases (TIMP) examined, the N-terminal domain of TIMP-3 (N-TIMP-3) inhibits ADAM12-S and ADAM12-PC with low nanomolar Ki(app) values while TIMP-2 inhibits them with a slightly lower affinity (9-44 nM). (ox.ac.uk)

The enzyme consists of the catalytic domain of human MMP-1 (Phe 100 -Gln 268 ) with a C-terminal purification tag. (enzolifesciences.com)

Unexpected effects of FERM domain mutations on catalytic activity of Jak3: structural implication for Janus kinases. (lu.se)

The crystal structures of human cdHDAC7 and its complexes with two hydroxamate inhibitors are the first structures of the catalytic domain of class IIa HDACs and demonstrate significant differences with previously reported class I and class IIb-like HDAC structures. (rcsb.org)

2022. Probing the mechanisms of two exonuclease domain mutators of DNA polymerase ε. (nih.gov)

Kinetic analysis revealed that the noncatalytic C-terminal domains are important regulators of Cm-Tf activity and that ADAM12-PC consisting of the pro domain and catalytic domain is the most active on this substrate. (ox.ac.uk)

The propeptide consists of approximately 80-90 amino acids containing a cysteine residue, which interacts with the catalytic zinc atom via its side chain thiol group. (wikipedia.org)
One of the two zinc ions is present in the active site and is involved in the catalytic processes of the MMPs. (wikipedia.org)
The second zinc ion (also known as structural zinc) and the calcium ion are present in the catalytic domain approximately 12 Å away from the catalytic zinc. (wikipedia.org)
Little is known about the roles of the second zinc ion and the calcium ion within the catalytic domain, but the MMPs are shown to possess high affinities for structural zinc and calcium ions. (wikipedia.org)
The Cys1 residue of the TIMP chelates to the catalytic zinc and forms hydrogen bonds with one of the carboxylate oxygens of the catalytic glutamate residue (Glu202, see mechanism below). (wikipedia.org)
IN is a 32 kDa protein with three distinct structural domains, the N-terminal zinc-binding domain, the central catalytic core domain and the C-terminal domain. (biomedcentral.com)

In the course of a fragment-based lead discovery effort aimed at finding ligands of the catalytic domain of UDP-glucose glycoprotein glucosyltransferase (UGGT), we encountered a mixture of trigonal crystals and pseudotrigonal triclinic crystals-with the two lattices closely related. (ox.ac.uk)

Here, we characterize the structure and enzymatic activity of the catalytic domain of human HDAC7 (cdHDAC7). (rcsb.org)

Genetic analysis of TMPRSS6 catalytic domain variants in Mexican patients with iron treatment refractoriness. (cdc.gov)

the membrane-binding domain consists of a series of amphipathic α helices with several hydrophobic amino acids exposed to a membrane monolayer. (wikipedia.org)

The hemopexin-like domain of MMPs is highly conserved and shows sequence similarity to the plasma protein, hemopexin. (wikipedia.org)
A member of this protein family, RipD (Rv1566c) from M. tuberculosis described in the present study, displays sequence alterations in the NlpC/p60 catalytic triad and carries a pentapeptide repeat at its C-terminus. (scilifelab.se)
In contrast with previously known members of the NlpC/p60 family, RipD does not show peptidoglycan hydrolase activity, which is consistent with the sequence alterations at the catalytic site. (scilifelab.se)

Among the mAbs, there were significant differences in extraction efficiency, ability to extract BoNT/A subtypes, and inhibitory effect on BoNT catalytic activity. (cdc.gov)

The crystal structure of the human RIG-I helicase domain, a key player protein in the antiviral innate immune response, is reported. (iucr.org)
Active Matrix Metalloproteinase-1 (MMP-1, interstitial collagenase, fibroblast collagenase) catalytic domain from human cDNA. (enzolifesciences.com)

Overall, this study demonstrates that three mutations located in the C-terminal region of the catalytic core domain of HIV-1 IN inhibit the IN-induced lethal phenotype in yeast by inhibiting the binding of IN to the host chromatin. (biomedcentral.com)
[ 4 ] Mutations have been identified throughout the gene, affecting all domains of the transcribed protein, most frequently the catalytic domain. (medscape.com)
Complex effects of naturally occurring mutations in the JAK3 pseudokinase domain: evidence for interactions between the kinase and pseudokinase domains. (lu.se)

The α chain of haemoglobin (α) has been crystallized as a homodimer bound to a domain from the S. aureus haemoglobin receptor iron-regulated surface determinant H (IsdH). (iucr.org)
Autosomal SCID caused by a point mutation in the N-terminus of Jak3: mapping of the Jak3-receptor interaction domain. (lu.se)

The crystal structure of the catalytic domain of the site-specific recombination enzyme gamma delta resolvase at 2.7 A resolution. (uchicago.edu)

By analyzing the single-molecule properties of engineered motors, we demonstrate that the non-catalytic domain has a key role in the motility mechanism by acting as a 'foothold' that allows Kar3 to bias translocation towards the minus end. (elifesciences.org)
These results demonstrate that the C-terminal region of the catalytic core domain of HIV-1 IN is important for binding to host chromatin and is crucial for both viral replication and the promotion of the IN-induced lethal phenotype in yeast. (biomedcentral.com)

and a hemopexin-like domain at the carboxy-terminal. (wikipedia.org)
This represents a naturally-occurring active form of MMP-9 which lacks the C-terminal hemopexin domain. (enzolifesciences.com)

We have previously demonstrated that MUC17 is anchored to the apical membrane domain via an interaction with the scaffolding protein PDZK1. (scilifelab.se)
A strong interaction of the catalytically inactive core domain with peptidoglycan is however retained, presenting the first example of the NlpC/p60 domains that evolved to a non-catalytic peptidoglycan-binding function. (scilifelab.se)

Furthermore, a different active site topology results in modified catalytic properties and in an enlarged active site pocket. (rcsb.org)
Both the cyclooxygenase and the peroxidase active sites are located in the catalytic domain, which accounts for approximately 80% of the protein. (wikipedia.org)

Using domain shuffling experiments, we present evidence showing that a central domain upstream of the conserved catalytic cysteine residue determines HopZ1 recognition specificity. (uea.ac.uk)

Enzymes carrying NlpC/p60 domains, for instance RipA and RipB from Mycobacterium tuberculosis, are bacterial peptidoglycan hydrolases that cleave the peptide stems and contribute to cell wall remodelling during cell division. (scilifelab.se)

The encoded protein contains an N-terminal motor domain which includes nucleotide-binding and microtubule-interacting regions, a stalk domain containing a predicted coiled coil motif and a C-terminal tail domain. (nih.gov)
In this study, we performed mutagenic analyses of the C-terminal region of the catalytic core domain of HIV-1 IN in order to delineate the critical amino acid(s) and/or motif(s) required for the induction of the lethal phenotype in the yeast strain HP16, and to further elucidate the molecular mechanism which causes this phenotype. (biomedcentral.com)

Catalytic properties of ADAM12 and its domain deletion mutants. (ox.ac.uk)

Our study identified three HIV-1 IN mutants, V165A, A179P and KR186,7AA, located in the C-terminal region of the catalytic core domain of IN that do not induce the lethal phenotype in yeast. (biomedcentral.com)

This structural conservation highlights the importance of the versatile domain composition of the RPF family. (iucr.org)

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. (cdc.gov)

Anatomy13

Organisms15

Chemicals and Drugs112

Analytical, Diagnostic and Therapeutic Techniques and Equipment38

Phenomena and Processes78

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science4

Health Care1

Subunit5

Subunits2

Recombinant2

Allosteric2

Residues7

Clostridium1

Cysteine-rich1

Epidermal1

Kinase domain6

Homologous1

Regulatory1

Tissue inhibitors of metalloproteinases3

Enzyme consists1

FERM1

Inhibitors1

Mechanisms1

Kinetic1

Zinc6

Glycoprotein1

Enzymatic1

Variants1

Consists1

Sequence3

Activity1

Human2

Mutations3

Receptor2

Gamma1

Demonstrate2

Hemopexin2

Interaction2

Active2

Recognition1

Bacterial1

Motif2

Properties1

Core domain1

Family1

Terms1