• Though many traditional sensing technologies for biomarker analysis and fundamental biological studies (i.e. enzyme-linked immunosorbent assays, real-time polymerase chain reaction, etc.) rely on single-parameter measurements, it has become increasingly clear that the inherent complexity of many human illnesses and pathways necessitates quantitative and multiparameter analysis of biological samples. (illinois.edu)
  • Enzyme-linked immunosorbent assays (ELISA) have been around as one of the primary methods of analyte detection for more than four decades. (ddw-online.com)
  • Over the years, cell sorting was combined with molecular biological techniques and proteomics for characterizing metastatic & primary cancer cell populations and single tumor cells. (grandviewresearch.com)
  • A molecular model of the FMDV 3A protein, derived from the nuclear magnetic resonance (NMR) structure of the poliovirus 3A protein, predicted a hydrophobic interface spanning residues 25 to 44 as the main determinant for 3A dimerization. (asm.org)
  • The defense of his dissertation on "Automated Isolation of Circulating Tumor Cells and their Molecular Biological Analysis" took place on January 30, 2023 at the Johannes Gutenberg University Mainz. (fraunhofer.de)
  • The aim of this thesis was to realize single cell isolation by means of automated immunomagnetic bead enrichment and antibody-based fluorescence detection in a chip-based microfluidic system and the molecular biological characterization of these cells to assay tumor features from liquid biopsy. (fraunhofer.de)
  • Under the original collaboration, which began in 1998, Idun developed primary molecular assays against targets in the core of the apoptosis pathway. (dragonroids.com)
  • GlioTex cell lines are characterized at the molecular level by high throughput molecular biology approaches (whole exome and whole transcriptome sequencing) allowing, for instance, to perform correlations between certain molecular alterations and response to compounds. (institutducerveau-icm.org)
  • I have 25 years of experience in the field of biological sciences, with specific knowledge of genetics, molecular biology and biotechnology. (roundtablegroup.com)
  • The screening process for affimers involves three iterations of phage display and ELISA assays. (news-medical.net)
  • An enzyme-linked immunosorbent assay (ELISA) kit was used to measure the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-10, vascular endothelial growth factor (VEGF) and transforming growth factor (TGF)-ß1. (bvsalud.org)
  • Over the years many changes to the basic format have resulted in assay improvements, but some of the most recent look set to take the traditional ELISA to new levels. (ddw-online.com)
  • These include many vendors now addressing the automation of ELISA assays. (ddw-online.com)
  • Other automated instrumentation is designed to support the creation/coating of ELISA assay plates or contains cartridge-based delivery of assay reagents to reduce set-up time. (ddw-online.com)
  • These are supported by a wide range of ELISA reagents, assay and kits providing relative profiling or absolute quantitation of proteins across a wide range of study areas. (ddw-online.com)
  • E nzyme-linked immunosorbent assays (ELISA) have been one of the primary methods of detecting the presence of a substance, usually an antigen, in an aqueous sample for more than 40 years. (ddw-online.com)
  • The immunoassay landscape has, however, dramatically changed in recent years with the development of reduced assay steps protocols, no wash ELISAs and new automated systems designed to run ELISA. (ddw-online.com)
  • This review article uses as its starting point some of the findings of HTStec's ELISA Assay Trends 2012 survey and report, published in June 2012 (1). (ddw-online.com)
  • This report discusses end-users' current practices and preferences in ELISA assays, and seeks to understand future user requirements. (ddw-online.com)
  • The main (primary) application area for survey respondents' ELISA assays was immunology (17% selecting). (ddw-online.com)
  • The most impressive performance gain detailed in the Nature Medicine paper is that the researchers have now demonstrated that the magnetic-nano sensor can successfully detect cancerous tumors in mice when levels of cancer-associated proteins are still well below concentrations detectable using the current standard methodology, known by the acronym ELISA. (phys.org)
  • The new method is also faster than standard ELISA assays, with results typically available in one to two hours. (phys.org)
  • 52 Measure focus using a high-sensitivity assay (e.g. (eprf.ca)
  • P erhaps most significantly are the big increases in assay sensitivity and the widening of the dynamic range accomplished by using alternatives to the traditional colorimetric detection, or by the use of new platform technologies. (ddw-online.com)
  • In the Nature Medicine paper, the researchers show that the new magnetic-nano sensor has a broad range of sensitivity, from part-per-billion levels to concentrations six orders of magnitude, or a million times, greater. (phys.org)
  • The idea that you could essentially, on a single assay platform, measure a broad diversity of biomolecules that are at such a wide range of concentrations with such sensitivity is really, truly remarkable," said Charles Drescher, a professor of obstetrics and gynecology at the University of Washington in Seattle, who was not involved with the research. (understandingnano.com)
  • All Luminex assays are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. (bio-techne.com)
  • Conclusions Htt AlphaLISA assays demonstrated robust performance, high sensitivity and broad dynamic range. (bmj.com)
  • Sensitivity less than 10.0 pg/mL for most assays! (antigenix.com)
  • First of all, let's not confuse the assay sensitivity with its clinical sensitivity. (medscape.com)
  • The assay sensitivity is what we do in the lab with limiting dilution of reagents, and we say, what's the least amount of molecules we can detect? (medscape.com)
  • Though this technology can be used to detect a variety of molecules, this dissertation establishes the utility of microring resonator chips for multiparameter analysis of several challenging protein targets in cell cultures, human blood sera, and other clinical samples such as cerebrospinal fluid. (illinois.edu)
  • Recent work studies the effect of electrical stimulation on neural stem cells seeded on the conductive scaffold and the pathways by which it enhances stroke recovery Further understanding the combined effect of electrical stimulation and stem cells in augmenting neural repair for clinical translational is a major focus of this research going forward. (stanford.edu)
  • The ability to create diagnostic assays and techniques enables us to understand biological systems more completely and improve clinical management. (stanford.edu)
  • Saitoh and co-workers demonstrated in a randomized, multicenter, double-blind, crossover, sham-controlled clinical study that daily repeated 5-Hz transcranial magnetic stimulation of primary cortex provided short-term pain relief in neuropathic pain patients ( 15 , 16 ). (frontiersin.org)
  • Recently, several clinical trials showed an increased left ventricular ejection fraction (LVEF) and an improved regional perfusion following intramyocardial application of cluster of differentiation (CD) 133 + stem cells [ 5 - 9 ]. (biomedcentral.com)
  • Yet, CD133 + stem cells for clinical use are purified using manual or semi-automatic devices based on the conventional magnetic cell sorting (MACS®) technique. (biomedcentral.com)
  • Although the basics of the magnetic detection technology used in the new biosensor were described last year in a paper in the Proceedings of the National Academy of Sciences , the new sensor is not only more sensitive than the previous one by several orders of magnitude, it also outperforms its predecessor, as well as detection methods now in clinical use, in several other ways. (phys.org)
  • The best existing analysis methods, or assays, in clinical use can detect proteins over a range of concentrations of at most two orders of magnitude. (phys.org)
  • The best existing analysis methods, or assays, in clinical use are able to detect proteins over a range of concentrations of at most two orders of magnitude. (understandingnano.com)
  • By combining the results from two-stage quantification, 4 or 5 orders of magnitude in dynamic range were achieved for the detection of the serotypes of BoNT/A, /B, /E, or /F. The effect of multiplexing the assay by mixing substrates for different BoNT serotypes into a single reaction was also investigated in order to reduce the numbers of the cleavage reactions and to save valuable clinical samples. (cdc.gov)
  • It possesses a universal sample-processing cartridge for conducting multiplexed assays, either nucleic acid or immunoassay-based, and is field deployable. (genengnews.com)
  • Magnetic Luminex Assays are the most flexible bead-based multiplex assays that we offer. (bio-techne.com)
  • The Luminex Discovery Assays allow for the maximum number of analytes in a multiplex and is supplied as a premixed kit. (bio-techne.com)
  • The principle of this approach is based on the combination of MB immunocapture-based enzyme activity assay and competitive immunoassay of the total amount of enzyme for simultaneous detection of enzyme inhibition and phosphorylation in biological fluids. (cdc.gov)
  • The ligand-binding assay methodology quantified the concentration of the hormone in the test material by comparing the effects of the test sample to the results of varying amounts of known protein (ligand). (wikipedia.org)
  • Ligand binding assays provide a measure of the interactions that occur between two molecules, such as protein-bindings, as well as the degree of affinity (weak, strong, or no connection) for which the reactants bind together. (wikipedia.org)
  • Some of the applications that have been tested using affimers include cell imaging, super resolution microscopy, protein function modulation, magnetic nanoparticle formation, and MRI reagent development. (news-medical.net)
  • This dissertation describes the fabrication, characterization, development, and application of silicon photonic microring resonator technology to multiplexed protein measurements in a variety of biological systems. (illinois.edu)
  • This can be attributed to the growing cellular analysis in the field of cancer, stem cells, drug discovery, protein engineering , and cell engineering. (grandviewresearch.com)
  • HDAC1 and vimentin were significantly increased in HCC cells, and HDAC1 overexpression enhanced vimentin mRNA and protein expression levels in an HDAC1 dose‑dependent manner. (spandidos-publications.com)
  • Vimentin is an intermediate filament protein in the cytoplasm of mesenchymal cells, and has frequently been used as a marker to demonstrate epithelial-mesenchymal transition, which is associated with the progression of cancer ( 19 ). (spandidos-publications.com)
  • The p53 protein is engaged in the repair of DNA mutations and elimination of heavily damaged cells, providing anticancer protection. (rcsb.org)
  • The Recombnant protein was conjugated to magnetic beads. (creativebiomart.net)
  • In this work, FMDV 3A homodimerization was evidenced by an in situ protein fluorescent ligation assay. (asm.org)
  • Replacements L38E and L41E, involving charge acquisition at residues predicted to contribute to the hydrophobic interface, reduced the dimerization signal in the protein ligation assay and prevented the detection of dimer/multimer species in both transiently expressed 3A proteins and in synthetic peptides reproducing the N terminus of 3A. (asm.org)
  • The viral particle is composed of a protein capsid that contains a positive-sense RNA molecule of about 8,500 nucleotides that is infectious and encodes a single polyprotein, which is processed in infected cells by cis - and trans -acting viral proteases ( 55 ) to yield different polypeptide precursors and the mature viral proteins ( 9 , 62 ). (asm.org)
  • When expressed as a recombinant protein in transfected cells, PV 3A cofractionates with endoplasmic reticulum markers ( 66 ), and its single transient expression can disrupt the secretory apparatus ( 23 ) and decrease major histocompatibility complex (MHC) class I expression ( 22 ). (asm.org)
  • The sensor that Wang and his colleagues created, which uses magnetic detection nanotechnology they had developed previously, can detect a given cancer-associated protein biomarker at a concentration as low as one part out of a hundred billion (or 30 molecules in a cubic millimeter of blood). (phys.org)
  • To create a multiplexed assay, Wang and his colleagues attached the magnetic-nano sensors to a microchip in an array of 64 sensors, each of which can be set up to detect a different protein. (phys.org)
  • This includes gene, protein and metabolic networks, cellular architecture and intracellular dynamics, cell communication and motility, cell division and differentiation, tissue formation and organogenesis, tissue and organ functions, changes in population characteristics as a consequence of interaction of organisms with their physical environment, with individuals of their own species, and with organisms of other species. (nih.gov)
  • Antibody pairs are selected and tested to confirm the parallel detection of natural and recombinant standard protein and to ensure the accurate determination of target analytes within biological samples. (bio-techne.com)
  • Chromatrap®, a business unit of Porvair Sciences, has published a new application note that describes how Chromatrap® ChIP-seq assays now enable unbiased, genome-wide understanding of protein-DNA regulatory networks. (labbulletin.com)
  • Compatible with direct, deep sequencing of enriched fragments, Chromatrap® ChIP-seq assays is shown to enable unbiased, genome-wide understanding of protein-DNA regulatory networks. (labbulletin.com)
  • 1 A new paper 1 reports on how the loss of autophagy along with beta cell expression of IAPP (amyloid islet polypeptide, also called amylin), a 37 amino acid protein coexpressed and released by pancreatic beta cells along with insulin, results in the death (apoptosis) of beta cells. (life-enhancement.com)
  • These toxins inhibit the release of neurotransmitters at the neuromuscular junction by cleaving soluble NSF attachment protein receptor (SNARE) complex proteins in nerve cells, which prevents the fusion of neurotransmitter containing vesicles to cell membranes 2 . (cdc.gov)
  • This ready-to-use, pre-coupled magnetic beads are in uniform particle size and narrow size distribution with large surface area, which is conducive to convenient and fast capture target molecules with high specificity and achieve magnetic separation. (creativebiomart.net)
  • In a prototype enrichment device (IsoMAG), we established an isolation protocol with EpCAM-targeting 1 µm magnetic beads for automated enrichment tested on different epithelial cancer cell lines. (fraunhofer.de)
  • The spin-column technology allows for centrifugation at each wash step, improving efficiency and minimising handling errors often associated with magnetic beads. (labbulletin.com)
  • For selective quantification of the WT htt, brain samples from BACHD mice were incubated with antibody-conjugated magnetic beads to deplete mutant htt. (bmj.com)
  • Assay uses antibody coated beads, Biotin Detection antibodies, 96 well filter plate, and R-PE signal for analysis. (antigenix.com)
  • Chemical and Biological Sensing Based on Gold Nanoparticles. (artechhouse.com)
  • Both enantiomers of the esterified form of the compound, as well as its corresponding carboxylic acids, were found active in fluorescence polarization (FP) assay, nuclear magnetic resonance (NMR) and microscale thermophoresis (MST) assay, with K i and K D values around 1 μm. (rcsb.org)
  • Abbott uses these assays for high-throughput screening of compound libraries and for compound design using Abbott's proprietary Structure Activity Relationships by Nuclear Magnetic Resonance (SAR by NMR) technology. (dragonroids.com)
  • In these assays, GlioTex monitors survival and tumour growth by magnetic resonance or bioluminescence imaging. (institutducerveau-icm.org)
  • Analysis was carried out by LC-MS/MS and Nuclear Magnetic Resonance NMR. (kingston.ac.uk)
  • Enzymatic cleavage assays represent an attractive approach where the functional active toxins are detected by monitoring the cleavage of their target substrates, or peptide mimics in most cases, by means of different techniques, such as immunological detection 18 , 19 , capillary electrophoresis 20 , fluorescence analysis 21 , Forster resonance energy transfer (ALISSA) 22 , and high performance liquid chromatography 23 . (cdc.gov)
  • 2. Subjects who have at least one measurable lesion per RECIST v1.1 criteria on imaging modalities (computed tomography scan or magnetic resonance imaging scan) by the local site Investigator assessment. (who.int)
  • The technique helps view cell pictures in 2D by stacking the 1D spatial profiles after demodulating the detected transmission and fluorescence signals. (grandviewresearch.com)
  • On the basis of technology, Fluorescence-based Droplet Cell Sorting (FACS) segment generated the highest revenue in 2018, as it is a highly accurate and precise sorting method. (grandviewresearch.com)
  • Moreover, this in-depth volume covers assay design and detection, including discussions on the design and implementation of in vitro assay studies using both fluorescence emission and magnetic contast. (artechhouse.com)
  • Automated platform properly suited for small molecules screening with immunomodulatory activity that relies on phenotypic assays using a range of cancer cells, reporter cells, humanized cells, primary nervous cells and immune system cells, animal models with invertebrates (zebrafish) and mammals, as well as on an instrument park for microscopy, fluorescence, mass imaging and mass cytometry. (cnr.it)
  • Specifically, despite the human body's endogenous receptors, hormones, and other neurotransmitters, pharmacologists utilize assays in order to create drugs that are selective, or mimic, the endogenously found cellular components. (wikipedia.org)
  • Fields such as medical diagnostics and cellular biology would benefit greatly from a technology that enables rapid, quantitative and reproducible assays for many targets within a single sample. (illinois.edu)
  • Those fluids include urine, saliva, blood plasma (blood with the blood cells removed), serum (blood plasma with the factors that promote clotting removed) and cell lysates (the name applied to the cellular stew produced by dissolution of cells). (phys.org)
  • Magnetic Nanoparticles assisted Cellular MR Imaging and their Biomedical Applications. (artechhouse.com)
  • The biconcave disk shaped human cells are devoid of a nucleus and of most cellular organelles. (biomedcentral.com)
  • Hence, these assays can be used as a powerful tool to quantify soluble htt variants in experimental studies using both cellular and tissue samples. (bmj.com)
  • Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity. (bio-techne.com)
  • In each assay, a pair of antibody was used to capture either total or mutant htt. (bmj.com)
  • Luminex Discovery Assays provide the most flexible assays on the market. (bio-techne.com)
  • Build your own Luminex Assay with our Luminex Assay Customization Tool. (bio-techne.com)
  • Luminex Assays are validated for use with cell culture supernatants, plasma and serum. (bio-techne.com)
  • The mouse discovery Luminex Assay is also validated for use with tissue lysates. (bio-techne.com)
  • A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay. (bio-techne.com)
  • Which Luminex Assay is Right For Me? (bio-techne.com)
  • Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays . (bio-techne.com)
  • The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. (bio-techne.com)
  • Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. (bio-techne.com)
  • The company's assay is a PCR-based method, in which cervical biopsy material is obtained from patients, amplified with several primer sets covering 16 HPV types, and then measured using a Luminex-based procedure. (genengnews.com)
  • Under this agreement, Abbott and Idun will continue their collaboration to discover drugs that regulate the activity of specific proteins involved in the cell pathways leading to programmed cell death, or apoptosis. (dragonroids.com)
  • This strategic initiative combines proprietary technologies from both Abbott and Idun in a major discovery effort to identify small molecule drugs that kill tumour cells by specifically targeting the apoptosis pathway. (dragonroids.com)
  • Secondary screening is carried out by Idun using its battery of proprietary biological apoptosis assays. (dragonroids.com)
  • Idun Pharmaceuticals, Inc. creates innovative human therapeutics with a primary focus on controlling apoptosis, or programmed cell death. (dragonroids.com)
  • We report a new approach for electrochemical quantification of enzymatic inhibition and phosphorylation for biomonitoring of exposure to organophosphorus (OP) pesticides and nerve agents based on a magnetic bead (MB) immunosensing platform. (cdc.gov)
  • Short-term patient-derived tumor explant culture (PDEC) systems offer potential benefits over reductionist cell cultures 10 , including tumor-specific genetic and phenotypic heterogeneity and opportunities to explore tumor cell behavior within the context of authentic tumor microenvironmental components (reviewed in 10 ). (nature.com)
  • This instrument integrates microfluidic, optical, computational, electrical, and mechanical technologies for sorting cells on the basis of their global phenotypic profiles and morphological & spatial properties, using an image-driven approach. (grandviewresearch.com)
  • Some newer types are called "mix-and-measure" assays because they do not require separation of bound from unbound ligand. (wikipedia.org)
  • According to researchers, a novel product based on microfluidic technique facilitates separation of circulating tumor cells from the peripheral blood of patients suffering from cancer. (grandviewresearch.com)
  • Historically, ligand binding assay techniques were used extensively to quantify hormone or hormone receptor concentrations in plasma or in tissue. (wikipedia.org)
  • It was assumed that non-thermal EMF may directly affect ion binding and/or transport, and possibly alter the cascade of biological processes related to tissue growth and repair ( 4 , 6 , 14 ). (frontiersin.org)
  • According to the European Medicines Agency (EMA) BM-derived CD133 + stem cells intended for the regeneration of human ischemic heart tissue are classified as an advanced therapy medicinal product (ATMP) [ 17 ]. (biomedcentral.com)
  • You gain a thorough understanding of membrane labeling, means of delivery QD and MNP Cargos inside live cells, the use of infrared emitting QDs for tissue imaging, deep tissue imaging with MNPs based on magnetic contrast, and toxicity issues associated with the use of inorganic probes to live cells and tissue. (artechhouse.com)
  • Exploring and Understanding the Toxicity of QDs and Nanoparticles to Cells and Tissue. (artechhouse.com)
  • Design and Use of Magnetic Nanoparticles as Constrast Agents for Tissue and Cell Imaging. (artechhouse.com)
  • Fine needle aspirates or biopsy, brushing, cell blocks, or tumour tissue from bone metastases that is subject to decalcification are not acceptable. (who.int)
  • Management and prognosis of disease requires the accurate determination of specific biomarkers indicative of normal or disease-related biological processes or responses to therapy. (mdpi.com)
  • In doing so, CTCelect enabled automated dispensing of single circulating tumor cells from HNSCC patient samples, qPCR-based confirmation of tumor-related biomarkers with a simplified one-step protocol and immunostaining using a panel of tumor markers. (fraunhofer.de)
  • For the pharmaceutical sector, the activities dedicated to the analysis, synthesis and biological evaluation of small bioactive organic molecules are able to support studies of lead compounds and preclinical development of lipids, natural products, peptides and biomarkers for oncology, immunology and neuroscience. (cnr.it)
  • The development of assays to validate candidate biomarkers is costly and time-consuming. (lu.se)
  • We used a previously validated multiplexed 10-min, targeted proteomic assay to assess 54 candidate cerebrospinal fluid (CSF) biomarkers in two independent cohorts comprising individuals with neurodegenerative dementias and healthy controls. (lu.se)
  • This targeted proteomic multiple reaction monitoring (MRM)-based assay can simultaneously and rapidly measure multiple candidate CSF biomarkers. (lu.se)
  • Subsequently, truncation and mutation of a vimentin promoter demonstrated that HDAC1‑induced vimentin expression was dependent on a nuclear factor κ‑light‑chain‑enhancer of activated B cells (NF‑κB) binding site in the vimentin promoter sequence. (spandidos-publications.com)
  • In the absence of adequate autophagy, the toxic effects of IAPP deposits induces endoplasmic reticulum stress that kills beta cells and can, in that way, lead to type II diabetes. (life-enhancement.com)
  • In the paper (Rivera, 2014) the researchers report on their experiments with rodent beta cell islets, showing that when autophagy was stimulated by the autophagy-inducing drug rapamycin that (at the dose used) beta cell IAPP content was reduced by 54% ±5.5% versus untreated (no rapamycin) cells. (life-enhancement.com)
  • Expertise and capability in functional genomics and DNA based assay design can be applied to numerous disciplines, research interests and diagnostic test development in the food or animal testing areas. (dcu.ie)
  • The biological activity of automatically isolated CD133 + cells was evaluated and compared to manually isolated CD133 + cells via functional assays as well as immunofluorescence microscopy. (biomedcentral.com)
  • In the biotechnology field, the Institute conducts research in the development and implementation of processes based on extremophilic and whole-cell enzymes for the biological mitigation of CO 2 and for the production of hydrogen, food ingredients and functional and/or bioactive compounds from the conversion of natural matrices and residues agro-food. (cnr.it)
  • Nonetheless the small number of CTCs compared to billions of blood cells in a patient sample requires sensitive methods to obtain reproducible tumor cell recovery rates. (fraunhofer.de)
  • Furthermore, we demonstrated that these automatically isolated cells bear proliferation and differentiation capacities comparable to manually isolated cells in vitro. (biomedcentral.com)
  • RIP140 is required for ERα-complex formation, ERα-mediated gene expression, and ERα-dependent breast cancer cell proliferation. (aacrjournals.org)
  • The efficacy of innovating compounds is evaluated in these models by assessing cell viability and proliferation. (institutducerveau-icm.org)
  • We use rodent models of stroke combined with biomaterial techniques, stem cell transplants, and microfabrication to achieve these aims and evaluate our methods with behavior testing and various imaging techniques. (stanford.edu)
  • or use data science methods and tools to deduce information about biological systems. (nih.gov)
  • Of interest are development of computational and mathematical algorithms and tools, modeling techniques and approaches for understanding the complexity of biological systems, and utilization of big datasets and data science methods for model construction. (nih.gov)
  • A summary of commonly used methods of measuring benzene in biological samples is presented in Table 7-1. (cdc.gov)
  • NS proteins are involved in crucial aspects of the viral cycle and pathogenesis, such as rearrangements of intracellular membranes required for endomembrane recruitment and the lysis of host cells ( 1 , 12 , 14 , 18 , 73 ). (asm.org)
  • There is enormous demand for lipids due to parasite growth inside the host cell [developing organelles (e.g. nucleus, mitochondria, food vacuoles and apicoplast) surrounded by membranes and accumulating lipids in lipid bodies] and subsequent replication. (biomedcentral.com)
  • Additionally, new lipid structures also appear inside the host cell: the parasite is surrounded by a parasitophorous vacuole membrane [ 24 ] and new parasite induced membranous structures (Maurer's clefts and transport vesicles) emerge, which play an important role in trafficking of parasite virulence factors to the surface of infected red blood cell (iRBC) membranes. (biomedcentral.com)
  • Cytokines are small signaling proteins that are present in serum and cell secretomes at concentrations in the pg/mL or ng/mL range. (illinois.edu)
  • They have also been used in biosensors and in cell cultures to manipulate cell signaling. (news-medical.net)
  • Specifically, I aim to elucidate the roles of oxidative stress and neuroinflammation in maintaining or disrupting blood brain barrier (BBB) integrity in ischemic stroke using in vitro cell cultures and in vivo models of transient or permanent middle cerebral artery occlusion (MCAO) in rats and mice. (ufl.edu)
  • A ligand binding assay (LBA) is an assay, or an analytic procedure, which relies on the binding of ligand molecules to receptors, antibodies or other macromolecules. (wikipedia.org)
  • Antagonizing MDM2 with small molecules restores the activity of p53 in p53 wild-type (p53 wt ) cells and thus provides positive outcomes in the treatment of p53 wt cancers. (rcsb.org)
  • Development of new or improved laboratory/experimental techniques, instruments, or supporting software that measure the location and dynamics of molecules in situ, and organelles, cells, or tissues on the nanometer and micrometer length scales. (nih.gov)
  • However, there are limited tools to quantify different forms of soluble full-length htt in biological samples in order to monitor the efficacy of the therapeutic molecules. (bmj.com)
  • The Institute has a proven experience in analyzing, preparing and evaluating the biological properties of small organic molecules. (cnr.it)
  • The efficiency sential y uniform after whole-body izing (i.e. removing electrons from) in causing damage and subsequent exposure to being highly heteroge- atoms or molecules of the medium biological effects is related not only neous in the case of non-uniform to the amount of energy transferred being traversed. (who.int)
  • The market is segmented into cell sorters, services, and reagents & consumables. (grandviewresearch.com)
  • With increasing applications of sorting technologies, cell labeling reagents have become widely available in the market. (grandviewresearch.com)
  • For example, affimers have been used in affinity assays, among others. (news-medical.net)
  • the technology will support the following areas: medical diagnosis at the single cell level, introducing drugs or genes into cells, genetic editing, carrying drugs to their destination inside the body, cleaning the environment from polluting particles, drug development, and creating a 'laboratory on a particle' - a microscopic laboratory designed to carry out diagnostics in places accessible only to micro-particles. (innovationtoronto.com)
  • cytokine quantitation is thus important in fundamental biological studies and diagnostics, and complex and overlapping cytokine roles make multiplexed measurements especially vital. (illinois.edu)
  • The foundations for which ligand binding assay have been built are a result of Karl Landsteiner, in 1945, and his work on immunization of animals through the production of antibodies for certain proteins. (wikipedia.org)
  • Affimers can be combined with traditional biological tests to increase our understanding of proteins. (news-medical.net)
  • For example, SH2 proteins expressed in mammalian cells are challenging to extract. (news-medical.net)
  • The researchers also demonstrated that the sensor is equally effective in every likely biological fluid, or matrix, that a doctor would want to analyze for cancer-associated proteins. (phys.org)
  • To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
  • Stiefel's thesis deals with detecting tumor features based on the automated isolation of circulating tumor cells from liquid biopsies and thus contributes significantly to establishing a platform technology of Fraunhofer IMM in applied research of liquid biopsy. (fraunhofer.de)
  • In vivo, stable ERα expression has been reported in patient-derived xenograft (PDX) models, especially in tumor cells introduced via intraductal transplantations 8 , 9 and these findings have suggested a strong microenvironment-dependent dynamic component in the regulation of ERα expression. (nature.com)
  • Cyclophosphamide did not show any inhibition of cell growth when it was added directly to target cells without prior vivo activation, whereas Epirubicin retained its cytotoxic potency for 3 days after the drugs storage in both the elastomeric infusion pumps and IV bags. (kingston.ac.uk)
  • Magnetic nanoparticles. (iyte.edu.tr)
  • Written and edited by leading experts in the field, this unique book places particular emphasis nanoprobes made of luminescent semiconductor nanocrystals (quantum dots or QDs) and magnetic nanoparticles (MNPs). (artechhouse.com)
  • Further, this cutting-edge book looks at the use of QDs and magnetic nanoparticles for live cell imaging and intracellular sensing. (artechhouse.com)
  • Colloidal Chemical Synthesis of Organic-Dispersible Uniform Magnetic Nanoparticles. (artechhouse.com)
  • Section III: Use of Magnetic Nanoparticles for Contrast Imaging and Sensing. (artechhouse.com)
  • His activity at NRL focuses on the development of inorganic nanoparticles and their interfacing with biological systems. (artechhouse.com)
  • Her research interests are the chemical analysis and the biological assessment of edible and pharmaceutical plants and beekeeping products, as well as the isolation and identification of their bioactive compounds. (imgnpp.org)
  • White blood cell contamination, usually hampering further downstream analysis of enriched tumor cells, was measured by FACS and minimized to about 1200 cells starting from 7.5 mL blood. (fraunhofer.de)
  • Once the desired cell is identified, the micro-robot captures it and moves it to an external instrument for further analysis. (inceptivemind.com)
  • Biostatistics areas of interest include development of advanced statistical techniques and methodologies for design of biological experiments, collection and analysis of the data from those experiments and interpretation of, and inference from, the results. (nih.gov)
  • Cytotoxic efficacy of each drug regimen was tested immediately after the drugs reconstitution and at intervals during the period of analysis, by exposing the human cancer cells to the final drugs dilution. (kingston.ac.uk)
  • This sample expert has over 25 years of experience and specialization in optics, with specific knowledge in flow cytometry, cell sorting, and hematology analysis. (roundtablegroup.com)
  • Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
  • B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)
  • Developing the micro-robot's ability to move autonomously was inspired by biological micro-swimmers, such as bacteria and sperm cells. (innovationtoronto.com)
  • Inspired by biological micro-swimmers, researchers developed the micro-robot, also called a micro-motor, that can move from place to place and perform various actions autonomously or through external control by an operator. (inceptivemind.com)
  • Against this background, the Fraunhofer Institute for Microengineering and Microsystems has recognized the unmet need of standardized CTC isolation and developed automated microfluidic platforms to enrich, detect and isolate single tumor cells from 7.5 mL whole blood. (fraunhofer.de)
  • Through the development of RIA technology, researchers have been able to move beyond the use of radioactivity, and instead, use liquid- and solid-phase, competitive, and immunoradiometric assays. (wikipedia.org)
  • Our new development significantly advances the technology in two main aspects: hybrid propulsion and navigation by two different mechanisms - electric and magnetic," explains Prof. Yossifon. (innovationtoronto.com)
  • In the journal Cell, in August 2018, researchers announced the development of Image-Activated Cell Sorting (IACS). (grandviewresearch.com)
  • Flow cytometry-based sorting plays a significant role in the development of therapeutic products due to its ability to analyze heterogeneous cell populations. (grandviewresearch.com)
  • The development of differentiated sexual stages (gametocytes) within human red blood cells is essential for the propagation of the malaria parasite, since only mature gametocytes will survive in the mosquito's midgut. (biomedcentral.com)
  • Remember that although the concepts are fundamentally the identical to those described in the last section for fungus cells, the task itself differs in relation to nuclei isolation and chromatin sample preparation considerably. (eprf.ca)
  • gauge needle with 1mL syringe - Magnetic rack for microcentrifuge pipes Nuclei isolation 1 Grow Drosophila S2 cells in T75 flasks with 15mL Comprehensive Schneiders moderate. (eprf.ca)
  • Flow cytometry-based sorting has been widely used by researchers in biology laboratories for cell isolation. (grandviewresearch.com)
  • 40 to 56.7 percent of cells were recovered after complete isolation from 7.5 mL untreated whole blood. (fraunhofer.de)
  • We highlighted the bottlenecks of microfluidic cell isolation in dependency of bead-binding on the cell surface and implemented a gating function for the cytometer subunit to selectively dispense cells instead of autofluorescent objects. (fraunhofer.de)
  • Micromotor actuation in biological samples by application of a magnetic field has become attractive. (inceptivemind.com)
  • They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. (bio-techne.com)
  • Dr. de Koning's research group evaluated panels of samples and observed that the accuracy was quite high, 98%, when the samples were tested in a comparison with a reference assay. (genengnews.com)
  • Aim We aimed to establish bioassays that can detect and selectively quantify the wild-type (WT) and mutant forms of htt in biological samples. (bmj.com)
  • Assay performance was tested in HEK293 cells, WT and BACHD brain homogenates. (bmj.com)
  • Results The AlphaLISA® platform was efficient at detecting endogenous and overexpressed forms of soluble htt in HEK293 cells and in brain homogenates. (bmj.com)
  • A detailed map of epigenetic marks and transcription factor binding is necessary for deducing the regulatory networks that underpin gene expression in a variety of biological systems. (labbulletin.com)
  • Biological tissues (brain, plasma, tumour) can be harvested for pharmacokinetic or pharmacodynamic studies. (institutducerveau-icm.org)
  • Sample Types: Use Serum/Plasma, Cell Culture Fluid, Cell Lysate, or other biological fluids. (antigenix.com)
  • ABSTRACT Multiple myeloma (MM) is a systemic malignancy of pathologic plasma cells that is treat able with chemotherapeutic agents and irradiation, but rarely curable. (who.int)
  • Multiple myeloma (MM) is a cancer of the The largest series, 23 patients with lepto- plasma cells. (who.int)
  • Malignant plasma cells in meningeal MM out of 2000 patients with bone marrow produce an immunoglobulin, MM, was reported was by Schluterman et al. (who.int)
  • The prevalence production, lytic lesions and increased of leptomeningeal infiltration in breast can- plasma cells in the bone marrow [ 1 ]. (who.int)
  • cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)
  • The use of cell lines does, however, introduce a risk the immunoglobulin (Ig)-secreting plasma cell in the spleen, of obtaining cell line-specific features as a result of the trans- gut, or BM [1, 2]. (lu.se)
  • HE staining revealed a gradual decrease in the number of inflammatory cells and healing of the wounds with increasing time after Nanocnide lobata extract administration. (bvsalud.org)
  • ChIP sample processing time is reduced to just 5 hours using up to 500,000 cells per IP. (labbulletin.com)
  • We invest time in the interpretation of complex spectral data to identify our compounds from natural sources, developing biological models to demonstrate their efficacy on health and understanding their biological role, implementing synthetic and biosynthetic pathways for their sustainable production. (cnr.it)
  • Assay Time approx. (antigenix.com)
  • The entire manufacturing process, the examination of cell number and viability of the final CP as well as the depletion of non-target cells were part of the validation process, which was performed in compliance with EU guidelines for GMP. (biomedcentral.com)
  • As a direct result of these monumental findings, researchers have continued the advancement of ligand binding assays in many facets in the fields of biology, chemistry, and the like. (wikipedia.org)
  • Sandy's comment: Do you ever wonder why some scientists, having discovered an important biological fact that connects other things in biology that had hitherto not been connected to it, begin to try to explain everything in terms of that newly discovered connection. (life-enhancement.com)
  • In cell culture systems, the luminal ERα + tumor cells are either outcompeted by other types of cells or the cells rapidly downmodulate ERα expression 5 . (nature.com)
  • Studies on the transcriptomic profiles of the clonal luminal breast cancer cell lines suggest that these cell lines do not recapitulate well the established luminal tumor subtype 7 . (nature.com)
  • Cancer metastases descend from proliferative circulating tumor cells (CTCs) which disseminate from the primary tumor via blood and lymph vessels. (fraunhofer.de)
  • That is a critical finding for us because it says that in a realistic biological application - that of tumor growth in mice - we can actually see tumors before anything else could have detected them," said Sanjiv Gambhir, M.D., Ph.D., principal investigator of the Stanford CCNE. (phys.org)
  • With the objective of accelerating the preclinical evaluation of new therapeutic approaches towards the clinic, GlioTex group has established more than 50 cell line models and murine models of human glioblastoma derived from the tumour of patients operated at La Pitié-Salpêtrière. (institutducerveau-icm.org)
  • Here the lipid profiles of red blood cells infected with the five different sexual stages of Plasmodium falciparum were analysed by mass spectrometry and compared to those from uninfected and asexual trophozoite infected erythrocytes. (biomedcentral.com)
  • A suite of 15 cell culture clean-rooms each equipped with Laminar Flow Cabinets, carbon dioxide incubators, inverted microscopes, etc. (dcu.ie)
  • Flow cytometry-based assay help quantifies and characterizes infected cells in HIV patients. (grandviewresearch.com)
  • Therefore, this work compares manual and automated immunomagnetic enrichment in terms of cell recovery in dependency of cell line and input number, bead characteristics, biofunctionaliza-tion protocol and blood cell contamination focusing on head and neck squamous cell carcinoma (HNSCC) and metastatic breast cancer. (fraunhofer.de)
  • Maurits de Koning, Ph.D., a scientist at DDL Diagnostic Laboratory discussed his company's efforts at validating a human papilloma virus genotyping assay. (genengnews.com)
  • Neurosyphilis, quite directly, is defined as a CSF WBC count of 20 cells/µL or greater or a reactive CSF Venereal Disease Research Laboratory (VDRL) test result. (medscape.com)
  • If no prior PDL1 result is available at Screening, the subject must be assessed for PD-L1 result prior to the date of Randomisation using the stated method (PDL1 IHC 22C3 pharmDx assay) at a qualified local laboratory. (who.int)
  • CD133 + stem cells were purified from patient-derived sternal bone marrow using the recently developed automatic CliniMACS Prodigy® BM-133 System (Prodigy). (biomedcentral.com)
  • Cell sorting using microfluidic technology is growing significantly due to its convenient and fast application as well as lower costs as compared to other techniques such as FACS and MACS. (grandviewresearch.com)
  • Due to the non-magnetic cell sorting technology, instruments with microfluidic technology speed up the process, sorting billions of cells per second. (grandviewresearch.com)
  • In the microfluidic benchtop platform CTCelect, we characterized each process step with two different carcinoma cell lines (MCF-7, SCL-1) demon-strating up to 87 Percent enrichment, 73 percent optical detection and dispensing efficiency. (fraunhofer.de)
  • Study which tests 2 medications (SB27, Keytruda) to compare how effective, safe and well tolerated in a large number of metastatic non-squamous non-small cell lung cancer patients. (who.int)
  • 4. Subjects who have NOT received prior systemic therapy (including cytotoxic chemotherapy, targeted therapy, or antineoplastic biological therapy) for their metastatic NSCLC. (who.int)
  • Another important innovation is the ability of the micro-robot to identify target cells that are not labeled - the micro-robot identifies the type of cell and its condition (such as degree of health) using a built-in sensing mechanism based on the cell's unique electrical properties. (innovationtoronto.com)
  • Total and mutant htt assays revealed differential htt expression in various brain regions (cortex, hippocampus, thalamus and cerebellum) of WT and BACHD mouse models. (bmj.com)
  • Another property, that of robustness, or the stability of the assay in the face of minor variations in conditions, was found to be excellent at 95%, with both cervical swabs and formalin-fixed tissues. (genengnews.com)
  • Membrane lipids of RBCs are believed to be the foundation of these cells' exquisite stability and deformability [ 21 ]. (biomedcentral.com)
  • Although the biological stability of anticancer drugs is a very useful assay, this study shows it may not correlate completely with the physicochemical stability of the drugs. (kingston.ac.uk)
  • The establishment of ERα + luminal breast cancer cell lines has turned out to be a challenging task for reasons not entirely clear. (nature.com)
  • We focus on developing conductive polymers for stem cell applications. (stanford.edu)