• This Sterile Highly Purified Water product is DEPC treated and manufactured to eliminate RNase enzyme activity. (severnbiotech.com)
  • These applications include next-generation sequencing, transfection, real-time PCR, measuring yields of RNA preparations for microarray experiments, reverse transcription PCR (RT-PCR), differential display PCR, northern blot analysis, S1 nuclease assays, RNase protection assays, and cDNA library preparations. (thermofisher.com)
  • The RiboGreen reagent is ideal for fast and accurate measurement of RNA before generating cDNA, northern blot analysis, S1 nuclease assays, RNase protection assays, reverse transcription PCR, and differential display PCR. (thermofisher.com)
  • An RNase protection assay for mapping the 5' end of mRNAs. (thelyonslab.com)
  • RNA synthesized from this kit is suitable for many applications including RNA structure and function studies, ribozyme biochemistry, probes for RNase protection or gel shift assays, hybridization-based blots, anti-sense RNA or RNAi experiments, microarray analysis, microinjection, sgRNA synthesis and in vitro translation studies. (neb.com)
  • The purified total RNA is suitable for use in a variety of downstream applications, including: RT-PCR, RT-qPCR, Northern blotting, nuclease protection assays, cDNA library preparation after poly (A)+ selection, and so on. (shoujiuji.com)
  • Reference RNA is ideal for microarray analysis, Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. (zyagen.com)
  • Other techniques include dot blotting, ribonuclease protection assays (RPAs), serial analysis of gene expression (SAGE), and differential or subtractive hybridization. (idtdna.com)
  • Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions Manon Gaudreault, Marie-Eve Gingras, Maryse Lessard, Steeve Leclerc, and Sylvain L. Guerin 3. (nhbs.com)
  • When using the Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific), we have found this underestimation to be a result of the standard used in the assay. (neb.com)
  • Nuclease S1 (EC 3.1.30.1) is an endonuclease enzyme that splits single-stranded DNA (ssDNA) and RNA into oligo- or mononucleotides. (wikipedia.org)
  • Here, we report the clinical validation of the specific high-sensitivity enzymatic reporter unlocking (SHERLOCK) assay using the enzyme Cas13a from Leptotrichia wadei for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-the virus that causes coronavirus disease 2019 (COVID-19)-in 154 nasopharyngeal and throat swab samples collected at Siriraj Hospital, Thailand. (nature.com)
  • Expression of the pDNA (R70pRomiLuc)-derived gene was investigated in cod tissues and cultures of cod aEEC by means of real-time RT-PCR and luciferase activity assay. (silverchair.com)
  • Aspergillus nuclease S1 is used in the laboratory as a reagent in nuclease protection assays. (wikipedia.org)
  • PicoGreen reagent assays provide a rapid and simple fluorescence-based method for the accurate quantitation of dsDNA. (thermofisher.com)
  • PicoGreen reagent assays are routinely used for genotyping by allele-specific PCR, quantitating dsDNA samples before and after PCR amplification, and determining PCR amplification yields before sequencing. (thermofisher.com)
  • OliGreen reagent assays are routinely used for antisense oligonucleotides, aptamers, genomic DNA isolated under denaturing conditions, PCR primers, sequencing primers, and single-stranded phage DNA. (thermofisher.com)
  • DNA footprinting utilizes a DNA damaging agent (either a chemical reagent or a nuclease) which cleaves DNA at every base pair. (lookformedical.com)
  • An extraction-less validation protocol was carried out to determine performance characteristics of the assay in both synthetic SARS-CoV-2 RNA as well as clinical specimens. (medrxiv.org)
  • Feasibility of the assay and analytical sensitivity was first determined by testing a dilution series of synthetic SARS-CoV-2 RNA in two different solvents (water and AMIES VTM), revealing a high degree of linearity and robustness in fluorescence readouts. (medrxiv.org)
  • Shortly after its identification, 2019_nCoV was renamed to the current consensus 2 term SARS-CoV-2 and the first complete SARS-CoV-2 RNA sequence was made public by the Chinese Centers for Disease Control and Prevention. (medrxiv.org)
  • Well-known versions include S1 found in Aspergillus oryzae (yellow koji mold) and Nuclease P1 found in Penicillium citrinum. (wikipedia.org)
  • Aspergillus nuclease S1 is a monomeric protein of a molecular weight of 38 kilodalton. (wikipedia.org)
  • Aspergillus nuclease S1 is known to be inhibited somewhat by 50 μM ATP and nearly completely by 1 mM ATP. (wikipedia.org)
  • Nuclease S1 (Aspergillus oryzae sp. (rockland.com)
  • Mung bean nuclease, similar activity but probably not homologous DNA/RNA non-specific endonuclease, non-homologous family with similar DNA/RNA activity but accepts double-stranded substrate better Balabanova LA, Gafurov YM, Pivkin MV, Terentyeva NA, Likhatskaya GN, Rasskazov VA (February 2012). (wikipedia.org)
  • S1 nuclease is an endonuclease that is active against single-stranded DNA and RNA molecules. (rockland.com)
  • The hybridization LC-MS/MS platform provided unique advantages, including minimal matrix effects and high specificity, compared with traditional ligand binding assays or liquid scintillation approaches, which enabled efficient and reliable in vitro protein binding assay. (aspetjournals.org)
  • The TPP1/POT1 heterodimer also promotes telomerase processivity, as demonstrated by an in vitro direct telomerase activity assay [ 4 , 20 ]. (prolekarniky.cz)
  • This zinc-dependent nuclease protein domain produces 5' nucleotides and cleaves phosphate groups from 3' nucleotides. (wikipedia.org)
  • The assay is many times more sensitive than conventional UV absorbance, and free nucleotides or short oligonucleotides of up to 6 bases do not interfere with the OliGreen ssDNA quantitation assay. (thermofisher.com)
  • For therapeutic applications, it is necessary to use modified nucleotides to incorporate nuclease resistance. (neoaptamers.com)
  • For therapeutic applications you need to use modified nucleotides at all positions in the selection process and this will result in lower synthesis costs on your final commercial aptamer than with a mixture of nucleotides and will provide the level of nuclease protection necessary. (neoaptamers.com)
  • (B) Western blot of total cellular lysates from DLD-1 BRCA2 −/− cells stably transfected with full-length BRCA2 cDNA constructs: BRCA2 Wild Type (WT) and BRCA2 R3052W (1 and 2 correspond to two independent clones). (frontiersin.org)
  • (F) Western blot of total cellular lysates from DLD-1 parental cells (these cells express a wild-type allele of BRCA2) stably transfected with R3052W (3 and 5 correspond to two independent clones) full-length 2XMBP-BRCA2 cDNA constructs. (frontiersin.org)
  • Familiarize yourself with critical parameters of qPCR assay design, specificity analysis, data interpretation, and troubleshooting. (idtdna.com)
  • The method provides substantial improvements, including minimal matrix effects and high specificity when compared with previously used oligonucleotide ƒ u detection methods such as ligand binding assays or liquid scintillation. (aspetjournals.org)
  • Using molecular modeling, biochemical and functional assays, as well as single-molecule time-lapse imaging, we show that RBPJL and RBPJ, despite limited sequence homology, possess a high degree of structural similarity. (uni-ulm.de)
  • Most nucleases with EC 3.1.30.1 activity are homologous to each other in a protein domain family called Nuclease S1/P1. (wikipedia.org)
  • Gene transfection into cells can, in fact, make those cells produce any kind of protein, with the right instructions, including monoclonal antibodies, designer receptors, anything imaginable. (expose-news.com)
  • Cell-free protein synthesis (CFPS) systems are increasingly being used as a fast, simple, and efficient method for biosensor engineering, decentralized manufacturing, and prototyping of genetic circuits 1 . (jove.com)
  • Identification of Protein/DNA Contacts with Dimethyl Sulfate: Methylation Protection and Methylation Interference Peter E. Shaw and A. Francis Stewart 9. (nhbs.com)
  • Specific protein-binding measures are often used as assays in diagnostic assessments. (lookformedical.com)
  • Cas9 nuclease can be converted into an RNA-guided DNA binding protein (dCas9) via inactivation of its two catalytic domains 12 , 13 and then fused to transcription activation domains. (cdc.gov)
  • Order ready-to-use PrimeTime™ qPCR Assays or you can design your own custom assay. (idtdna.com)
  • This assay is based on the release of acid soluble products from ssDNA through hydrolysis. (rockland.com)
  • Next GEM technology is built on a novel chip architecture that enables high-throughput single cell analysis with our Chromium instruments. (mosaiquedessavoirs.eu)
  • The surging COVID19 pandemic has underlined the need for quick, sensitive, and high-throughput SARS-CoV-2 detection assays. (medrxiv.org)
  • Chimpanzees share almost all their gene sequences with humans, and this closeness has made them ideal animal models for many human diseases. (the-scientist.com)
  • Overlapping of cloned or sequenced DNA to construct a continuous region of a gene, chromosome or genome. (umassmed.edu)
  • The standard in single cell gene expression The Chromium X, powered by Next GEM technology, can run the full breadth of single cell assays. (mosaiquedessavoirs.eu)
  • The assay, which we show is amenable to multiplexed detection in a single lateral-flow strip incorporating an internal control for ribonuclease contamination, should facilitate SARS-CoV-2 detection in settings with limited resources. (nature.com)
  • (C) Clonogenic survival analyses of BRCA2 WT versus the two independent R3052W clones after treatment with Olaparib, cisplatin, and mitomycin C. (D) Schematic of I-SceI nuclease-induced DSB HDR luciferase assay. (frontiersin.org)
  • In molecular biology, it is used in removing single stranded tails from DNA molecules to create blunt ended molecules and opening hairpin loops generated during synthesis of double stranded cDNA. (wikipedia.org)
  • Cationic lipids, like the lipid nanoparticles used in mRNA vaccines, are capable of transfecting basically any type of cell with instructions to make proteins. (expose-news.com)
  • Unlike a virus - which only binds to specific host factors expressed by specific cell lines and is endocytosed in those specific cells - cationic lipids, like the lipid nanoparticles ("LNPs") used in mRNA vaccines, are capable of transfecting basically any type of cell with instructions to make proteins. (expose-news.com)
  • Left panel: probit analysis performed with the final assay protocol. (cdc.gov)
  • G-quartet structures make excellent aptamers with binding affinities like RNA aptamers. (neoaptamers.com)
  • body fluids such as saliva, tears and mucus, can contain RNase's It is therefore very important to create laboratory regimes to control against contamination. (severnbiotech.com)
  • Whether you are performing your first cloning experiment or constructing multi-fragment DNA assemblies, NEB ® has the solution for you. (neb.com)
  • Ethanol is added to the flow-through, creating conditions that promote selective binding of RNA to the silica membrane. (shoujiuji.com)
  • The assays are many times more sensitive than conventional UV absorbance and can be optimized for different concentration ranges. (thermofisher.com)
  • Harnessing the adjuvant activity of cholera toxin (CT) could make this immunization method feasible, predicts Gregory M. Glenn, scientific director of IOMAI Inc., a biotech company in Washington, D.C. Glenn and colleagues are developing a technique that mixes the toxin with antigens to boost immune responses. (the-scientist.com)
  • Although many different methods to detect SARS-CoV-2 particles in clinical material have been developed, none of these assays are successful in combining all three of the above characteristics into a single, easy-to-use method that is suitable for large-scale use. (medrxiv.org)
  • In summary, we describe a rapid direct RT-PCR method to detect SARS-CoV-2 material in clinical specimens which can be completed in significantly less time as compared to conventional RT-PCR methods, making it an attractive option for large-scale SARS-CoV-2 screening applications. (medrxiv.org)
  • In this study, we have identified TIN2-R282H, the most common DC-causing mutation in shelterin subunit TIN2, as a separation-of-function mutant which impairs telomerase recruitment to telomeres, but not chromosome end protection. (prolekarniky.cz)
  • Notably, mutations in the TPP1 OB-fold domain compromise telomerase-dependent telomere extension but not telomere end protection [ 18 , 21 ], indicating that TPP1 performs a role in telomerase regulation which is distinct from its contribution to chromosome end protection. (prolekarniky.cz)
  • Neither co-transfection of protease and VLP constructs nor bicistronic vectors allowing expression of both protease and VLP separated by a cell cleavable linker improved VLP yield, and indeed many of the constructs showed significantly reduced VLP production. (bvsalud.org)
  • Unfortunately, the antiviral drugs are expensive and provide protection for only a short period of time. (justia.com)
  • Integrated DNA Technologies acquires Archer™ next generation sequencing research assays to advance actionable scientific discoveries. (idtdna.com)
  • 250 kb is easy to handle and can be accurately measured using standard spectrophotometric methods, UHMW DNA measurement can be made more accurate by reducing the overall DNA fragment size of a small aliquot of the sample. (neb.com)
  • The purpose is to deliver chemotherapy, immunotherapy, and/or radiation to eliminate malignancy, prevent rejection of new stem cells, and create space for the new cells. (medscape.com)
  • Additionally, the invention provides methods of making the compositions, as well as methods of introducing the nucleic acids into cells using the compositions and treating disease conditions. (justia.com)
  • Transcutaneous immunization methods in development aim to make their skin function like sponges. (the-scientist.com)
  • The Figure 1 shows oligonucleotide binding sites from representative strains taken from the full CCHFV alignment used for assay design. (cdc.gov)
  • The genetic complexity and low fertility of sugarcane under natural growing conditions make traditional breeding improvement extremely laborious, costly and time-consuming. (frontiersin.org)
  • And they are all looking for technology that is easy, rapid, and cost-effective for development and manufacture. (expose-news.com)
  • All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. (rockland.com)
  • We welcome your feedback and ideas to make the Registry a useful resource for the scientific community to fight against the pandemic. (asm.org)
  • We investigated whether expression of the Zika virus (ZIKV) and dengue virus (DENV) protease induced alterations in the cellular lipid profile, and subsequently whether co-expression of these proteases with VLP constructs was able to improve VLP yield. (bvsalud.org)
  • The ELECTRICAL manufacturing of this code is that of profiling, in a second making, the tidal faults was in the syrup on this expression, with underwater Instability to the deficient realm file for current techniques dc to much plants. (ligaya-technologies.com)
  • This, together with its vegetative propagation, which allows for stable transfer and multiplication of transgenes, make sugarcane a good candidate for crop improvement through genetic engineering. (frontiersin.org)
  • Once the autophagosome forms, it fuses with the lysosome and creates a structure called the autolysosome. (hindawi.com)