Mrna hybrid cells. Medical search. Frequent questions

With the success of COVID-19 vaccines, newly created mRNA vaccines against other infectious diseases are beginning to emerge. (mdpi.com)
The unprecedently speedy development of mRNA vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was enabled with previous innovations in nucleoside modifications during in vitro transcription and lipid nanoparticle delivery materials of mRNA. (mdpi.com)
Recent updates are briefly described in the status of mRNA vaccines against SARS-CoV-2, influenza virus, and other viral pathogens. (mdpi.com)
In closing, she highlights the robust T-cell response induced by mRNA vaccines, suggesting that cellular immunity against SARS-CoV-2 remains intact. (medscape.com)
We anticipate oral delivery of mRNA could facilitate rapid deployment of episodic interventions, such as vaccines, and support long-term therapies. (duke.edu)
This favorable safety profile makes mRNA especially attractive for vaccines and gene editing. (pipelinereview.com)
At least 16 mRNA vaccines and therapeutics are in clinical stages and a considerable number is in IND or pre-IND stage. (pipelinereview.com)
This report „ mRNA Vaccines & Therapeutics 2017: an industry analysis of technologies, pipelines, stakeholders and deals " as of June 2017 brings you up-to-date regarding key mRNA players, key mRNA technologies and applications, mRNA vaccines & therapeutics, business projects, business deals and funding opportunities. (pipelinereview.com)
The report highlights the value of mRNA vaccines and therapeutics in terms of partnering economic condition and equity financing rounds. (pipelinereview.com)
Through the establishment of potent humoral and cellular immunity, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) messenger ribonucleic acid (mRNA) vaccines offer protection against symptomatic infection. (sepoy.net)
According to recent research, booster vaccines strengthen CD4+ T-cell as well as memory B-cell responses against viral variants of concern (VOC), broaden the neutralizing antibody response, and improve neutralizing antibody potency. (sepoy.net)
The vaccines created by Moderna and Pfizer and BioNTech work by giving people's cells a strand of messenger RNA. (hhmi.org)
In the present episode I will give some background on mRNA vaccines, with some emphasis on a possible extension towards Influenza. (icpcovid.com)
The vaccines built around the novel mRNA technology are a highly visible example of this, alongside a multitude of new use cases for digital services. (mynewsdesk.com)
Revolutionary medical technologies such as mRNA vaccines or cell and gene therapy are opening up a whole new world of possibilities. (mynewsdesk.com)
To understand why the new mRNA vaccines are so different, a quick explanation of current vaccines is in order. (anthonycolpo.com)

It detected a total of 37,507 lncRNA probes and 32,825 mRNA probes and found 3593 differentially expressed lncRNAs and 3236 differentially expressed mRNAs. (biomedcentral.com)

The transcription of COX-1 yields a 2.7-kilobase (kb) messenger ribonucleic acid (mRNA) that encodes a 576-residue, 65-kd protein. (medscape.com)

Importantly, high magnitude T cell responses are generated after two vaccine doses, with the majority of the T cell response directed against epitopes that are conserved between the prototype isolate B and the VOC. (nature.com)
Here, we review the structural elements required for designing mRNA vaccine constructs for effective in vitro synthetic transcription reactions. (mdpi.com)
Unique features of mRNA vaccine platforms and future perspectives are discussed. (mdpi.com)
The researchers attempted to create an anti-melanoma vaccine by inserting different melanoma-associated antigens (MAAs) mRNA sequences into the MHC-II hybrid construct. (btxonline.com)
The report analyzes the mRNA vaccine and therapeutic pipelines and stakeholders in the field, especially technology companies and rare disease biopharma and major pharmaceutical companies. (pipelinereview.com)
A cohort of 66 individuals scheduled to be vaccinated with either a third dose of the BNT162b2 vaccine or the mRNA-1273 vaccine between October 2021 and March 2022 were recruited for blood donation at baseline (day 0), days 30 and 60 after vaccination. (sepoy.net)
The team employed spectral flow cytometry to evaluate B-cell responses to the booster vaccine with a 21-marker panel that included six tetramers for assessing responses against the spike protein subunit 1 (S1) of B.1, its receptor binding domain (RBD), and N-terminal domain (NTD), as well as identifying B cells that bound RBD and NTD of both B.1 and BA.1. (sepoy.net)
Mice that received a vaccine made from a hybrid spike protein resisted infection from several coronaviruses, researchers report. (hhmi.org)
By combining different parts of coronavirus spike proteins (one shown here), scientists created a new kind of mRNA vaccine. (hhmi.org)
While poly(I:C) is known to provoke a robust type I IFN, type III IFN, and Th1 cytokine response, its therapeutic use as a vaccine adjuvant is limited due to its vulnerability to nucleases and poor uptake by immune cells. (bvsalud.org)
This will prolong the expression of the mRNA, but it is quite possible that immune responses against the viral replicase will limit the response to a booster or to a 2nd, 3rd … vaccine encoding a different pathogen, but using the same replicase in a SAM format. (icpcovid.com)
A homologous 3rd mRNA vaccine dose in COVID-naïve individuals conferred greater cross-variant evenness of neutralization potency with stability that was equal to the hybrid immunity conferred by infection plus vaccination. (cdc.gov)

Improvements of lipid nanoparticle formulations as a vehicle for in vivo systemic delivery of mRNA has greatly favored the development of in vivo transfection strategies. (pipelinereview.com)

lncRNAs and mRNAs in the NP group (DCs isolated from NP) and the test group (DCs isolated from patients with AR) were identified via chip technology and bioinformatic analyses. (frontiersin.org)
Moreover, bioinformatic analyses were employed to identify the related biological functions of monocyte-derived DCs and construct the functional networks of lncRNAs and mRNAs that are differentially expressed (DE) in imDCs and mDCs. (frontiersin.org)
Quantitative RT-qPCR was used to study the significant fold changes of lncRNAs and mRNAs. (frontiersin.org)
In order to explore the expression profiles of dysregulated lncRNAs in cSCC and to reveal the potential roles of lncRNAs in the pathogenesis of cSCC, we performed microarray analysis to identify dysregulated lncRNAs and mRNAs. (biomedcentral.com)
Also, we intended to construct a lncRNA-mRNA correlation network for cSCC, which helps to establish bridges between lncRNAs and mRNAs to reveal the potential functional involvement of lncRNAs in cSCC pathobiology. (biomedcentral.com)

Absence of signal in NCI-H226 cells is predicted by RNAseq data and confirms the specificity of the antibody for BMF. (cellsignal.com)
In addition, it is not easy to introduce the solution of a polymer with the molecular weight greater than 120,000 like an antibody with high concentration into the cell because of its high viscosity. (justia.com)
and TH2 (T Helper 2) which has humoral immune functions, e.g., activation of B cells to produce antibody molecules to neutralize foreign matters and (See FIG. 94). (justia.com)
In a recent study posted to the medRxiv * preprint server, researchers assessed the impact of coronavirus disease 2019 (COVID-19) infection on B-cell and antibody responses to COVID-19 booster vaccination. (sepoy.net)
Study: Recent SARS-CoV-2 infection abrogates antibody and B-cell responses to booster vaccination. (sepoy.net)
In the present study, researchers examined how SARS-CoV-2 infection affects the antibody and B-cell responses to the third dose of the BNT162b2 or mRNA-1273 COVID-19 vaccination. (sepoy.net)
We analyzed SARS-CoV-2 variant recognition, dynamics of memory B cells and secreted antibody over time after infection, vaccination, and boosting. (cdc.gov)

A proliferation-inducing ligand (APRIL), also known as tumor necrosis factor ligand superfamily member 13 (TNFSF13), is a protein of the TNF superfamily recognized by the cell surface receptor TACI. (wikipedia.org)
This protein and its receptor are both found to be important for B cell development. (wikipedia.org)
To further characterize the function of DJ-1, we cloned cDNA encoding a novel DJ-1-binding protein, DJBP, by a yeast two-hybrid system. (nih.gov)
Immunoprecipitation of BMF protein from NCI-H209 cell extracts. (cellsignal.com)
We validated the performance of formulations and devices in rodents and pigs, demonstrating protein translation in the delta, gastric, and parietal cells of the gastric mucosa, in addition to systemic uptake. (duke.edu)
mRNA lacks genomic integration and its use results in transient expression of the encoded protein. (pipelinereview.com)
In heterotrimeric G-protein signalling, cell surface receptors (GPCRs) are coupled to membrane-associated heterotrimers comprising a GTP-hydrolysing subunit G-alpha and a G-beta/G-gamma dimer. (embl-heidelberg.de)
Mammalian Purkinje-cell protein-2 (Pcp2). (embl-heidelberg.de)
It may function as a cell-type specific modulator for G protein-mediated cell signalling. (embl-heidelberg.de)
Interaction of heterotrimeric G protein Galphao with Purkinje cell protein-2. (embl-heidelberg.de)
Pcp2 (Purkinje cell protein-2) was identified as a partner for Galphao in this system. (embl-heidelberg.de)
This mRNA serves as a template that cells use to manufacture the SARS-CoV-2 spike protein. (hhmi.org)
This hybrid protein absorbs blue light (major peak at 480 nm) and emits green light (major peak at 505 nm). (invivogen.com)
Additionally, ORF6 inhibits cellular mRNA export, resulting in the remodeling of the host cell proteome, and regulates viral protein expression. (bvsalud.org)
Dysregulated extracellular matrix (ECM) protein like elastin and collagen and depletion of vascular smooth muscle cells (VSMCs) of the aortic wall are believed as the main histopathological findings [ 3 - 5 ]. (hindawi.com)
A "helper" phosphatidylcholine and cholesterol are needed to stabilize the LNP in the extra-cellular milieu, but also enhance the release of the mRNA from the endosomes into the cytoplasm (where translation to the actual immunogenic protein can proceed). (icpcovid.com)
Electron microscopy of hybrids formed between adenovirus mRNA and a frag-ment of the adenovirus genome coding for the coat hexon protein was performed to locate the transcriptional unit. (brainkart.com)
Conversely, the transcription of COX-2 yields a 4.5-kb mRNA that encodes a 70-kd protein with roughly 70-75% homology to the COX-1 protein. (medscape.com)
Laboratory and bioinformatic investigations identified and validated 9 genetically related SARS-CoV-2 viruses with a hybrid Delta-Omicron spike protein. (cdc.gov)
Objective: This study evaluated the osteogenic induction of human bone marrow cells by human recombinant bone morphogenetic protein-4 (rhBMP-4) and proteins released by Saos-2 (human osteosarcoma cell line). (bvsalud.org)

This composite promoter comprises the Elongation Factor-1α (EF-1α) core promoter [3] and the R segment and part of the U5 sequence (R-U5') of the Human T-Cell Leukemia Virus (HTLV) Type 1 Long Terminal Repeat [4]. (invivogen.com)
The most plausible explanation for the existence of intervening se-quences is that they are the remnants of a parasitic sequence that spread through the genome of some early cell-type. (brainkart.com)
In order that the sequence not inactivate a coding region into which it inserted itself, the coding region arranged that it splice itself out of mRNA. (brainkart.com)
A) Target sequence within the zcdk9 mRNA 3′-UTR and MO hybridization positions. (echinobase.org)
A) Terminal sequence of the zcdk9 mRNA 3′-UTR and hybridization positions of antisense MOs . (echinobase.org)

Presently, several techniques for detecting mRNAs are available, which include in situ hybridization and polymerase chain reaction (PCR),' says David T. Leong , an Assistant Professor in the Department of Chemical & Biomolecular Engineering at National University of Singapore (NUS). (nanowerk.com)
Colonies containing plasmids with inserted sequences were initially screened by DNA dot hybridization, and those positive colonies were then screened by hybrid selected translation. (duke.edu)
PC8b cells treated with either dexamethasone or 8-bromo cyclic AMP yielded greater mRNATH hybridization on Northern blot analysis and accumulated higher molecular weight tyrosine hydroxylase RNA species. (duke.edu)
The virus provided a convenient source of DNA for hybridization reactions and RNA extracted from adeno-infected cultured cells was a rich source of viral RNA. (brainkart.com)
Figure 5.14 Hybridization of adenovirus hexon mRNA to adenovirus DNAfrom a region well upstream from the hexon gene. (brainkart.com)

Isolation of a cDNA clone for tyrosine hydroxylase mRNA. (duke.edu)
Poly(A+)-containing RNA from cells treated with both inducers was used to construct a cDNA library. (duke.edu)
1988). SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat. (invivogen.com)
Clones encoding the murine homologue were isolated from a murine T helper cell cDNA library. (ox.ac.uk)
By transfection of cell lines mc14 and mc20, the reactivating functions were shown to reside in the pAd2E1A-13 S cDNA clone of Ad2 DNA. (uni-bielefeld.de)

Subsequent electroporation of Cas9 mRNA results in transient CRISPR mediated DNA cleavage and highly efficient disruption of target loci. (ukri.org)

Nanowerk Spotlight ) Every cell is a busy messaging center, with thousands of genes churning out messenger RNA (mRNA) transcripts for translation into functional proteins. (nanowerk.com)
In new work, Leong and his team describe a new way of preparing functional DNA nanostructures that can provide accurate quantification and visualization of mRNA transcripts in living cells. (nanowerk.com)
Our research studied the lncRNA and mRNA expression profiles of monocyte-derived DCs and demonstrated the functional networks that are involved in monocyte-derived DCs-mediated regulation in AR. (frontiersin.org)
Utilizing electroporation to transfect mRNA constructs of modified antigen-specific TCRs, the researchers were able to express functional TCR on the surface of the quiescent T-cells and show activation by interaction with DCs loaded with the corresponding peptide antigen. (btxonline.com)
Type 1 diabetes results from the poorly understood process of islet autoimmunity, which ultimately leads to the loss of functional pancreatic beta cells. (springer.com)
We advance the hypothesis that antigen presentation by beta cells, their stress responses and functional heterogeneity are critical factors that will provide clues towards solving the enigma posed by islet autoimmunity in type 1 diabetes. (springer.com)
In vivo, human-murine anti-leukaemia modelling has confirmed functional integrity of CAR+TCR- cells, and found these were less prone to exhaustion compared to TCR+ cells. (ukri.org)
The MO removed the poly(A) tail and blocked zebrafish cdk9 (zcdk9) mRNA translation, showing functional mimicry between miRNA and MO. (echinobase.org)

Some examples include delivering DNA-free gene editing reagents such as ribonucleoproteins or mRNA, relying on reagent expression from non-integrated DNA, using novel delivery mechanisms such as viruses or nanoparticles, using unconventional selection methods to avoid integration of transgenes, and/or avoiding tissue culture altogether. (frontiersin.org)
The data reported in the paper demonstrate that siRNA complexed with SiSaf's silicon stabilized hybrid lipid nanoparticles (sshLNP) was able to significantly downregulate expression of an ADO2-specific mutant gene. (wane.com)
1. Novel hybrid silicon-lipid nanoparticles deliver a siRNA to cure autosomal dominant osteopetrosis in mice. (wane.com)
SiSaf's proprietary Bio-Courier™ delivery platform uses silicon stabilized hybrid lipid nanoparticles (sshLNP) to improve the stability, safety, and transfection efficiency of RNA. (wane.com)
Extracellular vesicles (EVs) are cell-derived membranous nanoparticles present in most biologic fluids. (cdc.gov)

the technology will support the following areas: medical diagnosis at the single cell level, introducing drugs or genes into cells, genetic editing, carrying drugs to their destination inside the body, cleaning the environment from polluting particles, drug development, and creating a 'laboratory on a particle' - a microscopic laboratory designed to carry out diagnostics in places accessible only to micro-particles. (innovationtoronto.com)
The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis found 16 significant regulated pathways in imDCs and 10 significant regulated pathways in mDCs, including the phagosome, cell adhesion signaling pathway, and inflammatory mediator regulation of TRP channels pathway. (frontiersin.org)
Furthermore, elevated levels of R-loops and of mRNA for paraspeckle genes in patient cells are linearly correlated with Multiple Myeloma progression. (uni-koeln.de)
Remarkably, hybrid molecules fail to form in tumor cells treated with EFV under the same conditions that repress proliferation and induce the reprogramming of expression profiles of coding genes, microRNAs (miRNAs) and ultraconserved regions (UCRs). (unife.it)
With the development of the gene expression profile, comparisons of differentially expressed genes that participate in the regulation of pathophysiological conditions in pathological tissue and normal tissue were available and convenient, and mRNAs gene expression profiles have been carried out in many diseases including TAD [ 6 ]. (hindawi.com)
Most of the trials so far have relied in collecting a patients own white cells and then using a disabled virus to fit new genes into a group of cells called T cells. (ukri.org)

Our method enables fast and sensitive high-resolution data acquisition, as demonstrated by recovering over 142 million transcripts from 453,843 cells from four mouse brain coronal slices in less than two days. (selectbiosciences.com)
Using this method, the research team successfully amplified full-length transcripts of 669 single HeLa S3 cells and 40 of them were randomly selected to perform single-cell RNA sequencing. (rna-seqblog.com)
mRNA transcripts for osteopontin were determined using RT- -PCR with specific primers. (bvsalud.org)
mRNA transcripts were detected in all culture conditions since the first day of culture. (bvsalud.org)

Furthermore, oral delivery enables local transfection of cells in the gastrointestinal tract not easily targeted via parenteral administration. (duke.edu)

Accordingly, here we analyse antibodies and T cells of a recently vaccinated, UK cohort, alongside those recovering from natural infection in early 2020. (nature.com)
In the case that the translation products of a gene are cell surface molecules, a method for selectively separating cells which have expressed the specific gene, while being viable, is to make fluorescence labeled antibodies bind to the surface molecules for labeling the cells fluorescently, to identify fluorescing cells by flow cytometry, and to separate the identified cells with a cell sorter (Fluorescence Activated Cell Sorter, FACS). (justia.com)
In addition, the panning method is also known wherein only the objective cells are absorbed on the bottom surface of a dish over which is covered with antibodies specifically binding to the cell surface molecules. (justia.com)
In this case, it is theoretically possible to fluorescently label the gene-expressing cells by introduction of fluorescence-labeled antibodies that are specific to the molecules localized in the cells into the cells, through microinjection and to separate the objective gene-expressing cells with the cell sorter described above based on the difference in fluorescence intensity of the cells with irradiation of laser beam or the like. (justia.com)
The number of the cells to which the labeled antibodies can be introduced for one experiment is at most ten or less. (justia.com)
This is because, during the process where polypeptide chains generated based on the genetic information are folded and secreted, their structure changes gradually and from time to time to prevent any known antibodies from binding to the polypeptide chains within or on the surfaces of live cells efficiently. (justia.com)
The first two doses of the BNT162b2 or mRNA-1273 vaccination produce very potent antibodies that can neutralize the ancestor virus. (sepoy.net)
Transfected cells can be analyzed for GFP expression by flow cytometry and by Western-blotting using GFP antibodies. (invivogen.com)
Experiments with monoclonal antibodies against CD2 suggest that CD2 is the cell surface receptor for a natural ligand involved in T cell proliferation. (ox.ac.uk)

Outside of the cell, Nano-SNEL can also be used to detect viral RNA that may be found in blood serum such as the Ebola virus. (nanowerk.com)
2015) Full-length single-cell RNA-seq applied to a viral human cancer: applications to HPV expression and splicing analysis in HeLa S3 cells . (rna-seqblog.com)

It not only provides a transcriptome characterization of HeLa S3 cells at the single cell level, but is a demonstration of the power of single cell RNA-seq analysis of virally infected cells and cancers. (rna-seqblog.com)

Because the targets - DNA, mRNA, miRNA - are ubiquitous in all biological systems, nano-SNEL is highly versatile as a diagnostic tool. (nanowerk.com)

There are 12 samples (6 TAD and 6 NT) that were used for microarray analysis by Arraystar Human mRNA microarray which contained 14596 mRNAs probes. (hindawi.com)

Additionally, Dr Lacombe reviews data on hybrid immunity - the immunity derived from a combination of infection and vaccination - and its ability to confer enhanced and long-lasting protection compared with either infection or vaccination alone. (medscape.com)
Hybrid immunity, or boosted immunity elicited by infection and vaccination, is also quite effective in defending against VOCs. (sepoy.net)

Part V deals with lymphocyte hybrids. (elsevier.com)
Furthermore, we have developed an automated process with selection techniques to generate CAR-T cells from umbilical cord blood T cells as well as conventional peripheral blood lymphocyte harvests from healthy donors. (ukri.org)
The murine homologue of the T lymphocyte CD2 antigen: molecular cloning, chromosome assignment and cell surface expression. (ox.ac.uk)
The human T lymphocyte antigen CD2 (T11, sheep erythrocyte receptor) is expressed on all peripheral T cells and all but the most immature thymocytes. (ox.ac.uk)

To search for novel proteins that interact with Galphao, a mouse brain library was screened using the yeast two-hybrid interaction system. (embl-heidelberg.de)
Interacts with TGA2, TGA3, TGA5 and TGA6 in yeast two hybrid assays. (phytoab.com)

Different MAAs were inserted into hybrid MHC-I and MHC-II constructs, and mRNAs were transfected into murine bone marrow derived dendritic cells (BMDCs) by electroporation using the BTX ECM 830 generator. (btxonline.com)

Folic acid and cyclic arginylglycylaspartic acid peptides were introduced to the surface of negatively charged lipid-coated hybrid polydopamine-cysteine cores for the delivery of epirubicin (EPI) (E/PCF-NPs). (dovepress.com)
An important key is the choice of the best "ionizable" lipid , that can bind the negatively charged mRNA. (icpcovid.com)

It was demonstrated in transient expression experiments that the 5' CCGG 3'-methylated late E2A promoter was at least partly reactivated in cell lines constitutively expressing the E1 region of Ad2 or of adenovirus type 5 (Ad5) DNA. (uni-bielefeld.de)

Conclusions: This study showed that an osteoblastic cell lineage may be obtained from human bone marrow derived from adherent cells, and that the presence of the rhBMP-4 seems to have an effect during the first stages of differentiation only. (bvsalud.org)

The modification of the three 5' CCGG 3' sequences at nucleotides +24, +6 and -215, relative to the cap site in this promoter, sufficed to silence the gene in transient expression either in Xenopus laevis oocytes or in mammalian cells, and after the fixation of the E2A promoter-chloramphenicol-acetyltransferase (CAT) gene construct in the genome of hamster cells. (uni-bielefeld.de)
L-type Ca 2+ channels (LTCCs) drive the bulk of voltage-gated Ca 2+ entry in vertebrate inner ear hair cells (HCs) and are essential for mammalian auditory processing. (jneurosci.org)
COX-1 is expressed constitutively and is isolated throughout most cell lines in almost all mammalian tissues. (medscape.com)
They can be released by various mammalian cells and pathogens, and their use as predictive biomarkers for disease progression and treatment outcomes has been reported for different pathologic conditions, including parasitic diseases ( 3 , 4 ). (cdc.gov)

For example, defective expression of mRNA linked to certain heterogeneous diseases such as cancer might be detectable at the single cell resolution using nano-SNEL. (nanowerk.com)
Further studies on different cancer types are needed to test if this mechanism is ubiquitously conserved and fundamental for cell homeostasis. (uni-koeln.de)
Nanodiamonds, with nitrogen-vacancy centers, and quantum dots are applied to develop quantum sensors for quantum switching intra vital imaging for iPS cell (induced pluripotent stem cells) based regenerative medicine, and quantum photo immuno-therapeutic devices for cancer. (selectbiosciences.com)
LINE-1 are poorly expressed in normal cells and abundantly in cancer cells. (unife.it)
Decreasing RT activity in cancer cells, by either LINE-1-specific RNA interference, or by RT inhibitory drugs, was previously found to reduce proliferation and promote differentiation and to antagonize tumor growth in animal models. (unife.it)
Using CsCl density gradients, we selectively identify Alu and LINE-1 containing DNA:RNA hybrid molecules in cancer but not in normal cells. (unife.it)
In cancer cells the abundant RT reverse-transcribes retroelement-derived mRNAs forming RNA:DNA hybrids. (unife.it)
HeLa is a well characterized HPV+ cervical cancer cell line. (rna-seqblog.com)
Cutaneous squamous cell carcinoma (cSCC) is the second most common type of skin cancer, the prognosis for patients with metastatic cSCC remains relatively poor. (biomedcentral.com)
Cutaneous squamous cell carcinoma (cSCC) is the second most common type of skin cancer, accounting for 20% of nonmelanoma skin cancers It is reported that approximately 1.8 million incidences of cSCC in a global context in 2017. (biomedcentral.com)
New treatments for leukaemia using gene therapy to re-program white bloods cells against cancer cells are showing a great deal of promise. (ukri.org)
We focus on the role of HPV in the increased incidence of oropharyngeal squamous cell carcinoma (OSCC), the head and neck cancer in which HPV is most commonly found ( 4 ). (cdc.gov)
Head and neck cancer most commonly is of the squamous cell carcinoma type (HNSCC) and includes cancers of the oral cavity, oropharynx, hypopharynx, larynx, sinonasal tract, and nasopharynx. (cdc.gov)
arations verified the presence of cancer cells in all samples. (lu.se)

Cell fate, function, and phenotype are significantly dictated through the spatiotemporal control of mRNA expression. (nanowerk.com)
Therefore, determining the mRNA expression of an individual cell can reveal critical insights into that particular cell's health and physiological state. (nanowerk.com)
However, these single-point and end-point techniques require the killing of the cells and are thus unable to capture the expression of mRNA in real time and locality with high precision. (nanowerk.com)
The objective of this study is to explore the long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) expression profiles of monocyte-derived dendritic cells (DCs) obtained from peripheral blood mononuclear cells (PBMCs). (frontiersin.org)
11. Flamigni F., Campana G., Carboni L., Guarnieri C., Spampinato S., Zinc is required for the expression of ornithine decarboxylase in a difluoromethylornithine-resistant cell line. (unibo.it)
Ca 2+ channel β subunits regulate cell-surface expression and gating of voltage-dependent Ca 2+ channel α1 subunits. (jneurosci.org)
Based on these data, they obtained a comprehensive understanding of the heterogeneity of HeLa S3 cells in gene expression, alternative splicing and fusions. (rna-seqblog.com)
Furthermore, the researchers identified a high diversity of HPV-18 expression and splicing at the single-cell level. (rna-seqblog.com)
We will combine our mRNAs expression profile (6 TAD tissues versus 6 non-TAD tissues) and GSE52093 downloaded from the Gene Expression Omnibus (GEO) database. (hindawi.com)
The two mRNAs expression profiles contained 13 TAD aortic tissues and 11 non-TAD tissues. (hindawi.com)
In the present study, we use mRNA microarray to acquire differential expression profiles in human TAD tissues and non-TAD tissues. (hindawi.com)
Genome editing technology is evolving rapidly and we have developed a self-inactivating lentiviral vector that tightly couples CAR expression with CRISPR guides through a hybrid 3' long terminal repeat region (LTR). (ukri.org)
Although splicing is a possible point for cells to regulate expression, there seemed no particular need for utilizing this possibility. (brainkart.com)
BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)

Table 1 provides a comparison between DNA and mRNA (encoding a particular antigen) as a vaccination strategy. (icpcovid.com)

Pilkinton in Acta Biomaterialia (June 2021) provides a nice didactic insight in the mechanism of action and recent advances in the technology that made mRNA so successful. (icpcovid.com)

Cell Death and Differentiation. (wikipedia.org)
Previous studies suggest that lncRNAs play critical roles in numerous biological processes, including epigenetic regulation, cell apoptosis cell cycle and cell differentiation regulation [ 6 ]. (biomedcentral.com)

Cells derived from bone marrow can undergo osteoinduction in vitro in the absence of osteoinductive factors such as bone morphogenetic proteins. (bvsalud.org)

Using this advanced technology, we uncover the cellular and subcellular molecular architecture of Alzheimer's disease, providing unprecedented insights into abnormal cellular behaviors and their subcellular mRNA distribution. (selectbiosciences.com)
The cell viability, reactive oxygen species (ROS) levels, ratios of oxidized nicotinamide adenine dinucleotide to its reduced form (NAD + /NADH), apoptosis assays, and cellular uptake of E/PCF-NPs were determined on 4T1 cells. (dovepress.com)

Scholars@Duke publication: Regulation of tyrosine hydroxylase mRNA by glucocorticoid and cyclic AMP in a rat pheochromocytoma cell line. (duke.edu)

The therapy will aim to induce molecular remission & thereby secure eligibility for allogeneic stem cell transplantation. (ukri.org)
Around 10-15% go on to develop relapsed refractory cases and may fail to respond to aggressive treatment, including allogeneic stem cell transplantation. (ukri.org)

In vivo experiments suggest an important role for APRIL in the long-term survival of plasma cells in the bone marrow. (wikipedia.org)

Prof. Gilad Yossifon explains that micro-robots (sometimes called micro-motors or active particles) are tiny synthetic particles the size of a biological cell, which can move from place to place and perform various actions (for example: collection of synthetic or biological cargo) autonomously or through external control by an operator. (innovationtoronto.com)

Compared to chemotherapy or photothermal treatment alone, the combined treatment in vitro significantly inhibited the survival rate of 4T1 cells to 17.7%, induced ROS generation, and reduced NAD + /NADH significantly. (dovepress.com)
In vitro infection of human primary macrophages highlights that SARS-CoV-2 entry is increased in cholesterol-loaded macrophages (foam cells) and is dependent, in part, on neuropilin-1 (NRP-1). (bvsalud.org)

Antisense morpholino targeting just upstream from a poly(A) tail junction of maternal mRNA removes the tail and inhibits translation. (echinobase.org)
In the present study, an antisense MO method is introduced that uses a 25-mer MO against a region at least 40-nt upstream from a poly(A) tail junction in the 3''-untranslated region (UTR) of maternal mRNA. (echinobase.org)
A PCR-based assay revealed MO-mediated specific poly(A) tail removal of zebrafish mRNAs, including those for cyclin B1, cyclin B2 and tbp . (echinobase.org)
The MO removed the elongated poly(A) tail from maternal matured mRNA. (echinobase.org)

The MO activity targeting cyclins A and B mRNAs was validated in unfertilized starfish oocytes and eggs. (echinobase.org)
This antisense method introduces a new application for the targeted downregulation of maternal mRNAs in animal oocytes, eggs and early embryos. (echinobase.org)

The murine CD2 gene was assigned to chromosome 3 by Southern blot analysis of mouse-hamster somatic cell hybrids. (ox.ac.uk)
Funk and co-investigators localized COX-1 to 9q32-q33.3 via somatic hybrid deoxyribonucleic acid (DNA) analysis. (medscape.com)

Fig 1 provides a nice overview of how mRNA stimulates the adaptive immune system. (icpcovid.com)

Injection of cdk9 , but not cdk9m, MOs into embryos inhibits polyadenylation and translation of zcdk9 mRNA during early development. (echinobase.org)

To overcome these challenges, we introduce SPRINTseq (Spatially Resolved and signal-diluted Next-generation Targeted sequencing), an innovative in situ sequencing strategy that combines hybrid block coding and molecular dilution strategies. (selectbiosciences.com)

The SV40 late polyadenylation signal enables efficient cleavage and polyadenylation reactions resulting in high levels of steady-state mRNA. (invivogen.com)

Universal T cells aim to circumvent HLA barriers and provide an 'off-the-shelf' alternative to autologous cell therapies. (ukri.org)

Dendritic cells (DCs), which are the most important antigen-presenting cells (APCs) that send signals to the T cells, mainly participate in the pathogenesis of many diseases with immunoregulatory mechanisms, such as AR. (frontiersin.org)
The skin is richer in antigen-presenting cells (dendritic cells or DC) than the muscle and therefore immune responses start already at the site of injection. (icpcovid.com)

13. Flamigni F., Campana G., Carboni L., Rossoni C., Spampinato S., Post-trascriptional inhibition of ornithine decarboxylase induction by zinc in a difluoromethylornithine resistant cell line. (unibo.it)
C) Specific translation inhibition of zcdk9 mRNA by cdk9 MO, but not cdk9m MO. (echinobase.org)

Primary carcinoma tissues and adjacent tissues were obtained from six patients with cutaneous squamous cell carcinoma who underwent surgical treatment at Institute of Dermatology, Chinese Academy of Medical Sciences. (biomedcentral.com)
A growing body of research shows that human papillomavirus (HPV) is a common and increasing cause of oropharyngeal squamous cell carcinoma (OSCC). (cdc.gov)

Tissues and Cells. (lu.se)

These results reveal the heterogeneity of a virus-infected cell line. (rna-seqblog.com)

Additionally, there were 601 DE lncRNAs and 168 DE mRNAs in the mDCs in the NP and test groups. (frontiersin.org)

Transfected BMDCs were injected into mice bearing melanoma tumors to assess the anti-tumor activity and T-cell activation stimulated by these modified DCs. (btxonline.com)
The long-circulating and higher tumor accumulation of E/PCF-NPs resulted in complete ablation of breast tumor tissue through the enhanced photothermal effect by NIR laser irradiation-mediated cell apoptosis. (dovepress.com)
Thus, the retrotransposon-encoded RT drives a previously unrecognized mechanism crucial to the transformed state in tumor cells. (unife.it)
Recent single-cell RNA-seq studies highlight the tumor heterogeneity present in most cancers, but virally induced tumors have not been studied. (rna-seqblog.com)

For example, the ability to manipulate and analyse minute volume of fluids and samples has led to a wealth of new biological assays which can provide unique approaches for better manipulation and capture of micro- and even nanoscale circulating biomarkers such as cells, extracellular vesicles and exosomes. (selectbiosciences.com)

have created a 3-D system that reconstitutes a tissue-like environment that allows for the integration of cytokines, DC and quiescent T-cells. (btxonline.com)
Spatial transcriptomics technology has revolutionized our understanding of cell types and tissue organization, opening new possibilities for researchers to explore transcript distributions at subcellular levels. (selectbiosciences.com)
It is described as a housekeeping enzyme, being responsible for cell-to-cell signaling, tissue homeostasis, and cytoprotection. (medscape.com)

They reported their findings in the April 23, 2015 online edition of ACS Nano ( 'Nature-Inspired DNA Nanosensor for Real-Time in Situ Detection of mRNA in Living Cells' ). (nanowerk.com)
When the cells were treated with nano-SNEL, we were able to attain robust in situ hybridized signals and the associated intracellular spatial distribution could be mapped since the cell itself is not destroyed. (nanowerk.com)
Recently, research has increased in the area of transplanting embryonic cells across species and growing kidneys and endocrine pancreas cells in situ. (medscape.com)

The chemi- appear to interact with the structural elements of the cell, cal analysis was assessed at DATA CHEM Laboratories using plasma- with apparent binding to the cytoskeleton [Porter et al. (cdc.gov)

In the case that the translation products of a gene are not cell surface molecules but localize in the cells (in the cytoplasm or in organella), the method described above cannot be adopted. (justia.com)
In the case that the translation products of the gene are not cell surface molecules, but molecules that are liberated into the extracellular fluid and that do not remain in the cell or near the cell membrane, it is very difficult to selectively trap the molecules to separate the cells expressing the specific gene, from other molecules with the approaches described above. (justia.com)
Also, even in the case that the translation products are present on the surfaces of cells, it is difficult to selectively separate the cells unless the molecules are specifically present on the surfaces of particular cells. (justia.com)

The associated immunodeficiency often involves impaired natural killer cell activity, absent delayed-type hypersensitivity, and a poor cell proliferation response to antigenic challenge. (medscape.com)

Treatment with E/PCF-NPs under irradiation induced 4T1 cell apoptosis in approximately 93.6% cells. (dovepress.com)

Western blot analysis of extracts from various cell lines using BMF (E5U2J) Rabbit mAb (upper) or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). (cellsignal.com)

Anatomy23

Organisms5

Diseases9

Chemicals and Drugs21

Analytical, Diagnostic and Therapeutic Techniques and Equipment20

Phenomena and Processes34

Information Science3

Vaccines16

Differentially expressed mRNAs1

Messenger ribonucleic acid1

Vaccine12

Delivery of mRNA1

LncRNAs and mRNAs5

Antibody7

Protein20

Sequence5

Hybridization5

CDNA5

Results in transient1

Functional8

Nanoparticles5

Genes6

Transcripts4

Transfection1

Antibodies9

Viral2

Characterization1

MiRNA1

Microarray analysis1

Immunity2

Lymphocyte4

Yeast two-hy2

Murine bone marrow1

Negatively charged2

Adenovirus1

Bone marrow derived1

Mammalian4

Cancer14

Expression15

Vaccination1

20211

Differentiation2

Proteins1

Cellular2

Scholars@Duke1

Allogeneic2

Vivo1

Synthetic1

Vitro2

Poly4

Oocytes2

Somatic2

Stimulates1

Inhibits1

Molecular1

Polyadenylation1

Autologous1

Antigen-prese2

INHIBITION2

Squamous cell carc2

Tissues1

Reveal1

Additionally1

Tumor4

Biomarkers1

Tissue3

Situ3

Structural1

Molecules3

PROLIFERATION1

Apoptosis1

Blot analysis1