Mrna cyclin dependent kinase inhibitor p27. Medical search. Frequent questions

p27(Kip1) is a cyclin-dependent kinase inhibitor. (biomedcentral.com)
The cyclin-dependent kinase (CDK) inhibitor p27(Kip1) is a key cell-cycle regulator of G1-to-S phase transition [ 1 ]. (biomedcentral.com)
In addition, BMP-2 overexpression induced p21 WAF1/CIP1 and p27 KIP1 expression, and cellular apoptosis in RCC cells. (oncotarget.com)
Phospho-p27 Kip1 (Thr198) Antibody detects endogenous levels of p27 Kip1 only when phosphorylated at Threonine 198. (affbiotech.cn)

The p27kip1 protein is involved in cell cycle regulation and belongs to the Cip/Kip family of cyclin dependent kinase(CDK)inhibitors. (wikipedia.org)

Mechanistically, overexpression of BCRC-3 induced the expression of cyclin-dependent kinase inhibitor 1B (p27). (biomedcentral.com)
Ectopic kinase overexpression in any culture cell line leads to polyploidy and centrosome amplification. (lookformedical.com)

The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. (cancerindex.org)
Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. (thermofisher.com)

These proteins were rescued by the proteasome inhibitor MG132, indicating the autocatalytic degradation of F-box proteins upon loss of CSN2 or CSN5. (biomedcentral.com)
The activity of CDKs is controlled by their binding to coactivator subunits termed Cyclins, as well as by CDK inhibitory proteins termed CKIs. (intechopen.com)
The accumulation of both Cyclin and CKI proteins is tightly regulated at the level of transcription. (intechopen.com)
In addition, Cyclin and CKI proteins are controlled at the level of their destruction. (intechopen.com)
E3 ligases are also key players in cell cycle control, mediating the degradation of cyclins, as well as cyclin dependent kinase inhibitor proteins. (e3ligasesignal.com)
One concentrate inside the mechanism of neuronal death in AD would be the aberrant expression of cell cycle related proteins, this kind of as cdc2, cdk4, cyclin B1, and cyclin D, which med iate cell cycle progression, in vulnerable neurons from the AD brain, these molecules perform important roles in neuronal death related with several paradigms of neuronal death. (fgfrinhibitors.com)
On top of that to cell cycle progres sion molecules, quite a few cell cycle inhibitors, such as p16 and p27, and tumor suppressor proteins such as p53 and BRCA1 are also increased in levels during the AD brain. (fgfrinhibitors.com)

The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). (thermofisher.com)
SnCs exhibit irreversible growth arrest accompanied by increased expression of cyclin-dependent kinase inhibitors (CDKi) such as p16 INK4a , and p21 Cip1 , accumulation of DNA damages, and secretion of diverse bioactive molecules known as the senescence-associated secretory phenotype (SASP). (aging-us.com)
The CDKN2A/B locus contains genes encoding cell cycle inhibitors, including p16 Ink4a , which have not yet been implicated in the control of hepatic glucose homeostasis. (diabetesjournals.org)

HDAC inhibition resulted in a transcriptional and posttranscriptional regulation of the cyclin-dependent kinase inhibitors p21(Cip1) and p27(Kip). (cret-signal.com)

Real-time quantitative PCR (RT-qPCR) was used to test the levels of circ_0003928, miR-136-5p and progestin and adipoQ receptor family member 3 (PAQR3) mRNA. (bvsalud.org)
Equipped with a mouse model of stress (5-HT1A-R-/- mice) and a 5-HT1A-R-expressing hippocampal neuron-derived cell line, HN2-5, our earlier studies have reported that a 5-HT1A-R-mediated signaling pathway functions via extracellular receptor-activated kinase 1/2 (ERK1/2)-catalyzed activation of protein kinase C alpha (PKC) to promote synaptogenesis at P15 in the hippocampal CA1 region [12,16]. (thetechnoant.info)
b) 4-Hydroxytamoxifen (but not tamoxifen), genistein (but not genistin), daidzein, and probably other nutritional and chemopreventive anti-cancer agents could up-regulate expression of p27 via receptor protein tyrosine kinases (RPTKs), phosphoinositide 3-kinase (PI3K), phosphoinosite-dependent kinase (PDK), Akt/PKB and mTOR. (biomedcentral.com)
Growth factors that signal through tyrosine-kinase receptor families include the epidermal growth factor (EGF), platelet-derived growth factor (PDGF) and transforming-growth factor-α (TGF-α). (janechin.net)
Transforming growth factor-β1 (TGF-β) signals through a serine/threonine-kinase receptor pathway. (janechin.net)
The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters. (lookformedical.com)
Although neither kinase-dead human epidermal growth factor receptor (HER)3 nor orphan HER2 can be activated by HER-related ligands on their own, the formation of HER2/HER3 heterodimers creates the most mitogenic and transforming receptor complex within the HER (erbB) family of transmembrane receptor tyrosine kinases. (biomedcentral.com)
However, it appears that regardless of the mechanism that triggers the formation of oncogenic HER2/HER3 heterodimers (HER2 over-expression or overall low HER2 but high levels of the HER3 ligand heregulin), HER3 transphosphorylation is a common response of breast cancer cells upon treatment with current inhibitors of the HER receptor tyrosine kinase network. (biomedcentral.com)
The notion that breast cancer disease can be viewed as a biological process that is driven by overactive human epidermal growth factor receptor (HER)1/2 receptor tyrosine kinases (RTKs) has led to development of various anti-HER tyrosine kinase agents. (biomedcentral.com)

Western blot analysis showed that Skp2-transfected cells expressed lower levels of p27 protein than the control cells. (elsevierpure.com)

The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. (cancerindex.org)
A family of highly conserved serine-threonine kinases that are involved in the regulation of MITOSIS. (lookformedical.com)
An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. (lookformedical.com)
Mammalian aurora-A belongs to a multigenic family of mitotic serine/threonine kinases comprising two other members: aurora-B and aurora-C. In this review we will focus on aurora-A that starts to localize to centrosomes only in S phase as soon as centrioles have been duplicated, the protein is then degraded in early G1. (lookformedical.com)
A family of serine/threonine kinase Aurora constitutes a key regulator in the orchestration of mitotic events. (lookformedical.com)

Also, the growth-inhibitory effects of HER1/2 tyrosine kinase inhibitors (TKIs) were previously found to be attenuated in the presence of heregulin, which is a high-affinity combinatorial ligand for HER3. (biomedcentral.com)
Several of these have undergone clinical trials, including low-molecular-weight inhibitors with highly selective and reversible tyrosine kinase inhibiting properties [ 1 - 5 ]. (biomedcentral.com)

S-phase kinase-associated protein 2 (Skp2), a member of the F-box family of substrate-recognition subunits of Skp1-Cullin-F-box ubiquitin-protein ligase complexes, is necessary for p27 ubiquitination and degradation. (elsevierpure.com)
This apparent increase in p27 protein expression might have been due to either increased synthesis or decreased degradation, or a combination of both [ 1 ]. (biomedcentral.com)
Like the endogenous HPK1, both wild-type HPK1 and its kinase-dead mutant, HPK1-M46, overexpressed in Panc-1 cells, were also targeted by proteasome-mediated degradation. (aacrjournals.org)
Shortening of poly(A) tail to 30 to 60 nucleotides in mammalian cellular material is necessary for mRNA degradation (30). (careersfromscience.org)
After deadenylation, hydrolysis of 5 m7G cover takes place, enabling degradation of decapped mRNA by 5-3 exoribonuclease, Xrn1 (31C33). (careersfromscience.org)

Hematopoietic progenitor kinase 1 (HPK1) regulates stress responses, proliferation, and apoptosis in hematopoietic cells. (aacrjournals.org)
have reported that cultured postmitotic cortical neurons exposed to Ab undergo apoptosis that may be dependent on tumor suppressor component ataxia telangiec tasia mutated action, whereas therapy with caffeine, that is an ATM inhibitor, can exert a neu roprotective effect on cultured neurons exposed to Ab. (fgfrinhibitors.com)

Transcriptional and translational control, sequestration in cyclin D1 complexes and localization all regulate p27 in G1 phase. (biomedcentral.com)
In the p53 pathway, the gene activates its transcriptional target p21 which is an inhibitor of cyclin / cdk2 as they work for the progression of replication and inactivates RB. (ijpsr.com)
I cause that the download Common knowledge for the subunit of other second mRNAs within Transcriptional interferons contains the 8B1 enzyme of chemical motif of carbonic precursors with their heat buildings. (erik-mill.de)

In general, the circRNA-miRNA-mRNA axis may function as an extensive regulatory network in progression of some diseases. (biomedcentral.com)
This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. (cancerindex.org)
Progression through the cell cycle is driven by the oscillating activity of Cyclin Dependent Kinases (CDKs). (intechopen.com)
The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. (affbiotech.cn)
The prevailing model during cell cycle progression is that cyclin-dependent kinase (CDK) activity increases during G1-S transition, which involves the formation of a cyclin-CDK complex. (biomedcentral.com)
In detail, CCNE (cyclin E, including CCNE1 and CCNE2) binds to CDK2, and subsequently phosphorylates Rb to promote G1-S progression. (biomedcentral.com)
Transphosphorylation of kinase-dead HER3 and breast cancer progression: a new standpoint or an old concept revisited? (biomedcentral.com)
Failure to reverse breast cancer progression despite apparent inhibition of HER1 and HER2 kinase functions in cell-based assays as well as in patient tissues and tumours is an enigma that is not yet resolved [ 6 ]. (biomedcentral.com)

Real-time qPCR was used to quantify mRNA transcripts of 26 genes associated with ruminal epithelial function. (biomedcentral.com)
Far more recently, a genome wide pathway meta analysis with PD tissues has specifically recognized a set of genes controlling cellu lar bioenergetics and mitochondria biogenesis that have been selleck inhibitor shared by each brain and blood. (fgfrinhibitors.com)

Amino acid deficiencies also up-regulate the expression of p27 using some components of this pathway. (biomedcentral.com)
Although it is kinase defective, the HER family member HER3 can be phosphorylated by HER1 or HER2, and HER3 can couple with the pro-survival phosphatidylinositol-3-OH kinase (PI[ 3 ]K)/Akt pathway directly whereas HER1 and HER2 cannot [ 5 ] (Figure 1 ). (biomedcentral.com)

Treatment of Panc-1 cells with a proteasome inhibitor, MG132, increased the HPK1 protein levels in a dose-dependent manner, suggesting that alteration in proteasome activity contributes to the loss of HPK1 protein expression in pancreatic cancer. (aacrjournals.org)

cyclin D1 mRNA expression and cyclin D1 promoter activity. (cret-signal.com)
Finally, we provide evidence that these observations are applicable in vivo by demonstrating that HDAC inhibition decreased neointima formation and expression of cyclin D1 in a murine model of vascular injury. (cret-signal.com)
Reduced expression level of p27, a cyclin-dependent kinase inhibitor, is associated with high aggressiveness and poor prognosis of various malignant tumors, including gastric carcinoma. (elsevierpure.com)
In the present study, we examined the clinical and biological significance of Skp2 expression in human gastric carcinoma and the relationship between the expression of Skp2 and p27. (elsevierpure.com)
These findings indicate that Skp2 expression can modulate the malignant phenotype of gastric carcinoma, possibly via p27 proteolysis. (elsevierpure.com)
The expression levels of circRNAs, mRNAs and miRNAs in human tissues and cells were detected by quantitative real-time PCR (qRT-PCR). (biomedcentral.com)
Furthermore, MJ significantly increased the expression of BCRC-3, resulting in an obvious up-regulation of p27. (biomedcentral.com)
Some circRNAs contain miRNA response elements (MREs) and function as competing endogenous RNAs (ceRNAs) to interact with miRNAs and regulate the expression of target mRNAs. (biomedcentral.com)
CiRS-7 has more than 70 miR-7 binding sites and thus acts as effective miR-7 suppressor to regulate the expression of miR-7 target mRNAs. (biomedcentral.com)
This report addresses the question of whether various nutritional and chemopreventive anti-cancer agents up-regulate the expression of p27 in preneoplastic and neoplastic cells. (biomedcentral.com)
Experimental evidence presented in the first half of this report shows that these agents fairly faithfully up-regulate expression of p27 in mouse epidermal (JB6) and human breast cancer (MCF7, MDA-MB-321, and AU565) cells. (biomedcentral.com)
Up-regulation appears to be specific to p27 because expression of cyclin D1, E, and A, and p21Cip1/Waf1 was not modulated by these agents. (biomedcentral.com)
Up-regulation of the expression of p27 is likely due to the activation of translation rather than transcription of p27 because (a) up-regulation is mediated by the 5'-untranslated region (-575) of the p27 gene and (b) the antibiotic actinomycin D, an inhibitor of transcription, did not attenuate the up-regulation of p27. (biomedcentral.com)
c) Expression of p27 could also be up-regulated via RPTKs followed by MAPKs - MEK, ERK and p38MAPK - and probably MNK. (biomedcentral.com)
Finally, (d) global hypomethylation of 5'-m 7 G cap of mRNAs could also up-regulate expression of p27. (biomedcentral.com)
Based on these findings, we conclude that various nutritional and chemopreventive anti-cancer agents up-regulate expression of p27 in (pre)neoplastic cells. (biomedcentral.com)
Preliminary studies using either N -methyl- N -nitrosourea (MNU)-induced rat breast cancer model or human breast cancer cell lines in vitro had suggested, but not proved, that nutritional and chemopreventive anti-cancer agents increase p27 protein expression. (biomedcentral.com)
This study provided evidence that the up-regulation of p27 protein expression is at least in part due to increased synthesis and that this increase fairly faithfully recapitulates the cancer preventive activity of nutritional and chemopreventive anti-cancer agents. (biomedcentral.com)
Preliminary studies using in vivo model of MNU-induced rat mammary cancer and in vitro model of cultured cells had suggested - but not proved - that various nutritional and chemopreventive anti-cancer agents, including moderate dietary restriction, up-regulated the expression of p27 (Fig. 1a ). (biomedcentral.com)
A significant association of BMP-2 mRNA expression was also found with advanced tumor stage and lymph node involvement, while lower BMP-2 mRNA expression was significantly associated with poor overall survival after radical nephrectomy. (oncotarget.com)
BMP-2 mRNA expression was significantly enhanced in RCC cells by 5-aza-2'-deoxycitidine treatment. (oncotarget.com)
The prevalence of BMP-2 promoter methylation was significantly greater and BMP-2 mRNA expression was significantly lower in RCC samples as compared to normal kidney samples. (oncotarget.com)
After MG132 withdrawal, wild-type HPK1 protein expression was markedly decreased within 24 hours, but kinase-dead HPK1 mutant protein expression was sustained for up to 96 hours. (aacrjournals.org)
Furthermore, restoring wild-type HPK1 protein in PDA cells led to the increase in p21 and p27 protein expression and cell cycle arrest. (aacrjournals.org)
Several hallmarks of cellular senescence, such as cell cycle arrest, expression of cyclin-dependent kinase inhibitors, DNA damages, and senescence-associated secretory profile were evaluated. (aging-us.com)
Polyvinyl pyrrolidone-coated silver nanoparticles in a human lung cancer cells: time- and dose-dependent influence over p53 and caspase-3 protein expression and epigenetic effects. (sciendo.com)
By 5 µM TRO for 24 hours, the mRNA, protein expression and activity of catalase were increased in all three cell lines. (e-roj.org)
The change of radiation sensitivity by combined with TRO is not dependent on the PPARγ expression level. (e-roj.org)
This growth inhibition by TRO is linked to the G1 phase cell cycle arrest through the up-regulation of the cyclin-dependent kinase inhibitors p21 and p27 [ 14 - 16 ] and/or repression of cyclin D1 expression [ 17 , 18 ]. (e-roj.org)
Pioneering studies showed that high expression of kinase-dead HER3 can predict early escape from the anti-HER2 monoclonal antibody trastuzumab. (biomedcentral.com)

This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. (cancerindex.org)
CDK4 is a member of the Ser/Thr protein kinase family. (thermofisher.com)
Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. (affbiotech.cn)

The p27 cis-regulatory element is a structured G/C rich RNA element which is involved in controlling cell cycle regulated translation of the p27kip protein in human cells. (wikipedia.org)
Both the open reading frame and the stem loop elements contribute to cell cycle-regulated translation of the p27 mRNA. (wikipedia.org)
BCRC-3 functions as a tumor inhibitor to suppress BC cell proliferation through miR-182-5p/p27 axis, which would be a novel target for BC therapy. (biomedcentral.com)
When up-regulated, p27 inhibits G1-to-S phase transition of the cell cycle. (biomedcentral.com)
There was no difference in HPK1 mRNA levels in PDA cell lines or primary PDA compared with those in HPDE cells or ductal epithelium in chronic pancreatitis and normal pancreas, respectively. (aacrjournals.org)

Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA. (affbiotech.cn)
Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. (affbiotech.cn)

Aurora kinase C is a chromosomal passenger protein that interacts with aurora kinase B in the regulation of MITOSIS. (lookformedical.com)
An aurora kinase that localizes to the CENTROSOME during MITOSIS and is involved in centrosome regulation and formation of the MITOTIC SPINDLE. (lookformedical.com)
An aurora kinase that is a component of the chromosomal passenger protein complex and is involved in the regulation of MITOSIS. (lookformedical.com)

We explored 36 mRNA-seq libraries in order to study transcriptome dynamics during proliferation and differentiation processes at 6 time points. (biomedcentral.com)

This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). (thermofisher.com)

Further studies were conducted to gain some insight into the molecular basis of this increase in the synthesis of p27. (biomedcentral.com)
Rather all observations of receptors and factors are the reliable tyrosines but at best the kinase can initiate the loop and synthesis of the ricin, be the gene or significance on Text-to-Speech to bind physiological site. (erik-mill.de)

Treatment of vector control K562 cells with the autophagy inhibitors 3-methyladenine and bafilomycin A1 recapitulated the growth kinetics, vacuolar morphology and LC3-II accumulation of CSN2 knockdown cells indicating that the cellular phenotype of CSN2 cells arises from autophagy inhibition. (biomedcentral.com)
The legislation of mRNA balance enables cellular material to rapidly adapt to environmental adjustments (27, 28). (careersfromscience.org)

These inhibitors possess an N-terminal CDK-inhibitory domain which binds to the ATP binding pocket of the kinase and modulates its function. (wikipedia.org)
The zinc finger domain of Tzfp binds to the tbs motif located at the upstream flanking region of the Aie1 (aurora-C) kinase gene. (lookformedical.com)

Importantly, BCRC-3 could directly interact with miR-182-5p, and subsequently act as a miRNA sponge to promote the miR-182-5p-targeted 3'UTR activity of p27. (biomedcentral.com)
To address this question, the effects of various nutritional and chemopreventive anti-cancer agents on the activity of the proximal 5'-upstream region of p27 gene were investigated by transient transfection assay. (biomedcentral.com)
in particular, HER3 signalling buffered against incomplete inhibition of HER2 kinase activity has been suggested to be the mechanism that allows HER2 over-expressing breast cancer cells to escape HER TKIs. (biomedcentral.com)

The particular mRNA amounts of E-cadherin, Occludin along with JAM1 from the Windows xp stepped in organizations as well as Computer group significantly ascended (R smaller as compared to 2.05) that can be a involving N-cadherin revealed a climbing down from inclination (G bigger compared to 0.05). (e3ligasesignal.com)

Signaling specificity is conferred by receptors and mediated through associated-kinases. (janechin.net)

This p27 cis-regulatory element is 114 nucleotides in length and is located at the very 5' end of the 5'UTR of the p27 mRNA. (wikipedia.org)
Certainly, mRNAs of some regulatory substances, such as for example c-myc, cyclins, p27, cyclooxygenase-2 (Cox-2), and interleukin 2 (IL-2), are short-lived normally, and their balance is certainly subject to alter upon external arousal (29). (careersfromscience.org)
Regulatory ZEB1-SCRIB Never-ending loop in order to recuperate epithelial phenotype and also apical junctional complicated could possibly be one of the elements in which Exp creates the anti-metastasis impact.Riboswitches are usually normal ligand-sensing RNAs typically that are based in the 5′ UTRs associated with mRNA. (e3ligasesignal.com)

Vitamin D is also known to exert rapid effects that are not dependent on gene transcription [ 5 ]. (biomedcentral.com)

Metformin (MTF) has been reported to target NLK (Nemo-like kinase) to inhibit non-small lung cancer cells. (cancerindex.org)
Because kinase-inactive HER3 is not presently an amenable target for forthcoming HER TKIs, molecular approaches that can efficiently block heregulin-triggered HER3 transactivation or nucleocytoplasmic trafficking of heregulin might offer novel strategies with which to manage HER-driven breast cancer disease. (biomedcentral.com)

Works in various organisms have revealed that the kinase is involved in centrosome separation, duplication and maturation as well as in bipolar spindle assembly and stability. (lookformedical.com)

Agonist (100 nM 8-OH-DPAT) (D) treatment of freely dividing HN2-5 cells caused stimulation of PKC (Physique 1a) [25,26,27,28,29], which was blocked by the 5-HT1A-R antagonist WAY100635 (WAY) (10 M) but not by an inhibitor of the ERK1/2 kinase MEK (U0126) (U) (10 M) (Physique 1a). (thetechnoant.info)
Those transformed cells form tumours when implanted in immunodeficient mice, indicating that the kinase is an oncogene. (lookformedical.com)
Increasing evidence indicates that senescent cells could be a promising new target for therapeutic intervention known as senotherapy, which includes depleting senescent cells, modulating SASP and restoration of senescence inhibitors. (frontiersin.org)

Numerous instructional classes involving riboswitches have been discovered, enabling mRNA being governed through diverse and from a physical standpoint crucial mobile metabolites and also small molecules. (e3ligasesignal.com)

Somatostatin , peptide with 14 amino acids, 3 mg per day, injected slowly in the evening after supper, subcutaneously or intravenously with a 12-hour timed syringe (evening administration is indispensable since this coincides with the nocturnal peak in GH and GH-dependent growth factors). (beatingcancercenter.com)

Aurora kinases are found in all organisms in which their function has been conserved throughout evolution, namely the control of chromosome segregation. (lookformedical.com)

Anatomy8

Organisms3

Chemicals and Drugs74

Analytical, Diagnostic and Therapeutic Techniques and Equipment5

Phenomena and Processes29

Disciplines and Occupations1

Information Science3

Kip14

P27Kip11

Overexpression2

Including D-type cyclins2

Proteins7

INK4a3

Cip11

Receptor9

Levels of p271

Serine5

Tyrosine-kinase2

Degradation5

Apoptosis2

Transcriptional3

Progression8

Genes2

Pathway2

Proteasome inhibitor1

Expression30

CDK43

Cell5

Cdk22

Regulation3

Differentiation1

Phosphorylation1

Synthesis2

Cellular2

Binds2

Activity3

0.051

Receptors1

Regulatory3

Exert1

Cancer2

Stability1

Cells3

Standpoint1

Growth1

Found1