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ImmunophenotypingLymphocytesLymphocyte ActivationFlow CytometryLymphocyte SubsetsT-LymphocytesLymphocyte CountB-LymphocytesAntigens, CDLaser Scanning CytometryAntigens, CD7Antigens, CD19LeukemiaAntigens, CD3Antigens, CD5Antigens, CD45Antigens, CD20Lymphoma, B-CellCD4-Positive T-LymphocytesAntibodies, MonoclonalLymphoma, T-CellLeukemic InfiltrationCD4-CD8 RatioLymphomaSpleenAntigens, SurfaceCell SeparationLeukocyte CountLymph NodesLymphocyte Culture Test, MixedBone MarrowCells, CulturedLeukemia, Prolymphocytic, T-CellT-Lymphocyte SubsetsHematologyCD8-Positive T-LymphocytesLymphocytes, Tumor-InfiltratingIn Situ Hybridization, FluorescenceKiller Cells, NaturalImage CytometryPhytohemagglutininsLymphoma, Mantle-CellAntigens, NeoplasmLymphoma, Non-HodgkinClone CellsB-Lymphocyte SubsetsT-Lymphocytes, CytotoxicImmunohistochemistryLymphocyte TransfusionLymphoproliferative DisordersAntigens, Differentiation, T-LymphocytePseudolymphomaAntigens, CD4LeukocytesTumor Markers, BiologicalPhenotypeCD4 Lymphocyte CountNeprilysinPrecursor Cell Lymphoblastic Leukemia-LymphomaLeukemia, Lymphocytic, Chronic, B-CellConcanavalin AHematologic NeoplasmsAntigens, CD56Leukemia, Hairy CellCytotoxicity, ImmunologicMonocytesHLA-DR AntigensAcute DiseaseMycosis FungoidesLymphatic DiseasesBurkitt LymphomaNeoplasm, ResidualSplenic NeoplasmsPlasma CellsAntigens, Differentiation, B-LymphocyteBlood CellsBone Marrow CellsInterleukin-2Leukemia-Lymphoma, Adult T-CellGene Rearrangement, gamma-Chain T-Cell Antigen ReceptorBone Marrow ExaminationLeukocytes, MononuclearLymphoma, Large B-Cell, DiffuseLeukemia, Myeloid, AcuteAntigens, Thy-1Leukemia, MyeloidAntigens, DifferentiationBiopsy, NeedleImmunoenzyme TechniquesLymphocyte DepletionMitogensPrognosisImmunity, CellularHodgkin DiseaseAntigens, CD34Cell DifferentiationImmunoglobulin Light ChainsTranslocation, GeneticMice, Inbred C57BLReceptors, Lymphocyte Homing
SubsetsPeripheral blood lymphSubpopulationsCytotoxic T lymphocytAssaysBone marrowCharacterizeTumorLymphoidCellsMalignantLeukemiaFlowDiagnosisLymph nodesPercentageMonocytesCytoplasmEnumerationLymphomaLeukemiasClinicalImmuneGastrointestinalCountsIsolationCellBloodMonoclonalQuality assuranceAIDSPatientsFindingsPrimaryInfectionAbsoluteSpecific
Subsets10
Peripheral blood lymph3
  • To determine the immunologic characteristics of tumor infiltrating lymphocytes (TILs) in comparison with the corresponding peripheral blood lymphocytes (PBLs) of patients with ovarian cancer in order to detect specific antitumor-reactive-immunocompetent cells. (nih.gov)
  • Peripheral blood lymphocytes had a higher proliferative activity and cytotoxicity against natural killer-sensitive tumor cells than the corresponding TILs. (nih.gov)
  • Peripheral blood lymphocytes showed more potentially valuable behavioral characteristics than TILs. (nih.gov)
Subpopulations5
  • It utilizes an advanced proprietary flow cytometry method, which combines cutting-edge laser technology with monoclonal antibodies, to precisely measure the properties of living cells such as size, shape, density and granularity which results in a precise count of various lymphocyte subpopulations. (lymphocytemap.com)
  • Lymphocytes and their subpopulations are the key elements of the immune system. (lymphocytemap.com)
  • It is the numbers, ratios, percentages and activity level of these lymphocytes, and their subpopulations which are responsible for protection against various environmental factors, including viruses. (lymphocytemap.com)
  • Immunophenotyping identified circulating CD8 + T-cell subpopulations, including tumor-reactive (T TR ), effector memory, central memory (T CM ), effector, and naïve T cells from samples collected before and after mdSBRT. (elsevierpure.com)
  • Objective: Investigate the dysfunction of B lymphocyte subpopulations in people living with HIV/AIDS, hospitalized at the Tropical Medicine Foundation - Heitor Vieira Dourado, with or without a frequent history of hospitalization. (fiocruz.br)
Cytotoxic T lymphocyt1
  • Adoptive transfer of polyclonal cytotoxic T lymphocytes (CTLs) specific for CMV or EBV seems promising, but it is unclear whether this strategy can be extended to adenovirus, which comprises many serotypes. (nih.gov)
Assays2
  • This document has been developed by CDC to give guidance to laboratories performing lymphocyte immunophenotyping assays in human immunodeficiency virus-infected persons. (cdc.gov)
  • As director of a clinical flow cytometry laboratory, she oversees the design, validation, and implementation of various immunophenotyping assays in addition to ensuring quality assurance and regulatory compliance for CLIA certification. (stanford.edu)
Bone marrow3
Characterize1
Tumor2
  • Tumor infiltrating lymphocytes and PBLs were phenotyped by their surface markers, cytokine pattern, proliferation rate, and cytotoxic ability. (nih.gov)
  • Her research and clinical interests include clinical assay development for tumor immunophenotyping, lymphocyte subset monitoring, and immunotherapy target antigen assessment in a variety of hematologic and immunologic disorders. (stanford.edu)
Lymphoid2
  • Differentiating between low-grade lymphoma and reactive lymphocytes is often difficult by morphology alone as reactive lymphoid cells may acquire activation morphology from being exposed to different cytokines within the body fluid. (cytojournal.com)
  • In one report, NIAID Director Anthony Fauci explained: "Although most studies necessarily focus on HIV infection of peripheral-blood mononuclear cells, the lymphocytes that are in the peripheral blood at any given time represent only about 2 percent of the total lymphocyte pool, most of which is in the lymphoid organs. (sidasante.com)
Cells18
  • HIV infection is characterized by a decrease and, eventually, a depletion of CD4+ T-lymphocytes (helper T cells). (cdc.gov)
  • Using immunophenotyping, HIV-positive blood samples and age-matched controls were tested for the proportion of lymphocytes that are T cells, B cells, natural killer (NK) cells, CD4+ T cells (helper T cells), and CD8+ T cells (suppressor/inducer T cells). (cdc.gov)
  • and the percentage of lymphocytes that are CD4+ T-cells. (cdc.gov)
  • Immunophenotyping was performed on BAL cells and lung-associated lymph node cells. (cdc.gov)
  • Immunophenotyping and expression profiling suggested that p73 loss allowed increased maturation of malignant B cells and deregulated genes involved in lymphocyte homing and dissemination of human lymphomas. (jci.org)
  • The lymphocytes at these effector websites are principally T cells which current necessary morphological, phenotypical and purposeful variations. (ccc-flow.org)
  • Lymphomas are cancers of a specific type of white blood cells known as lymphocytes. (merckmanuals.com)
  • Anti-CD20 can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, and B cell localization in tissues. (biogenex.com)
  • Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin's disease, particularly of lymphocyte predominant type. (biogenex.com)
  • By immunostains, the lymphocytes are predominantly T cells, and plasma cells are polyclonal, expressing both kappa and lambda light chains. (cap.org)
  • However, these neoplasms have a distinct nodular appearance without abundant lymphocytes and plasma cells typically seen in Hashimoto thyroiditis. (cap.org)
  • or=26% T cells were found in the biopsy material whereas scid tumours showed a paucity of T lymphocytes. (lshtm.ac.uk)
  • The percentage of CD16/CD56 + T cells was higher in the large granular lymphocyte or atypical T-cell fractions in sHV, but no TCR Vα24 invariant chain characteristic of NKT cells was detected. (bvsalud.org)
  • Although the exact mechanism of the altered proportions of CCR4+/CD3+ and central memory CD4+ cells in the gastric mucosa of patients with cancer is unknown, focusing on lymphocytes in the gastric mucosa might help improve our understanding of gastric cancer development after H. pylori eradication. (bvsalud.org)
  • Total absolute cell count and percent lymphocyte values are usually obtained using the number of white blood cells and lymphocytes counted on an automated hematology instrument multiplied by the percentage of positivelystained cells measured on a flow cytometer. (beckman.com)
  • Natural Killer (NK) cells are lymphocytes of the innate immune system, originally described by their capacity to control tumour cells and eliminate virus-infected cells. (ox.ac.uk)
  • Mononuclear cells were separated from peripheral blood using a Ficoll gradient for ex vivo and in vitro immunophenotyping. (fiocruz.br)
  • A classification of lymphocytes based on structurally or functionally different populations of cells. (bvsalud.org)
Malignant1
Leukemia1
Flow10
  • The process of measuring the percentage of CD4+ T-lymphocytes in the whole blood sample is referred to as ``immunophenotyping by flow cytometry'' (9-14). (cdc.gov)
  • Flow cytometric immunophenotyping is a highly complex and relatively new technology. (cdc.gov)
  • This document provides CDC recommendations for immunophenotyping by flow cytometry. (cdc.gov)
  • Flow cytometry, also called immunophenotyping, is the most important test to confirm a diagnosis of CLL. (cancer.net)
  • The feasibility of lymphocyte isolation and flow cytometry using a single endoscopic biopsy specimen from the gastrointestinal tract of patients who have undergone hematopoietic stem cell transplantation has not been investigated. (bvsalud.org)
  • We divided the flow cytometry samples into two groups: group A, successful lymphocyte isolation (n=24), and group B, incomplete isolation (n=27). (bvsalud.org)
  • This Standard (Indirect) Method, however, is associated with wide interlaboratory variation.1-4 Flow-Count Fluorospheres is an efficient alternative to the Standard (Indirect) Method for absolute counts and provides this measure directly using the single platform of flow cytometric immunophenotyping analysis. (beckman.com)
  • To monitor immune exhaustion, we performed immunophenotyping on blood specimens by using multicolor flow cytometry the day before and 5 weeks after treatment initiation. (cdc.gov)
  • B lymphocytes were phenotyped by CD19, CD27, CD70 markers and intracellular IgM production by flow cytometry. (fiocruz.br)
  • Diagnosis is by flow cytometry and immunophenotyping of peripheral blood. (msdmanuals.com)
Diagnosis1
  • Asymptomatic, elderly patients with early stage, uncomplicated CLL do not require extensive investigation beyond confirmation of the diagnosis by CBC, film, and immunophenotyping. (streamliners.co.nz)
Lymph nodes1
  • Zymosan-treated rats also had a smaller PMN infiltration in the lung compared to control, however, a greater number of lymphocytes was present in lymph nodes of the rats. (cdc.gov)
Percentage3
  • Single-platform technology (SPT) is designed to enable de- system and managing the health care of persons infected with terminations of both absolute and percentage lymphocyte sub- human immunodeficiency virus (HIV) ( 1-4 ). (cdc.gov)
  • The systems also provide the CD4/CD8 ratio when using CD45-FITC/ CD4-RD1/CD8-ECD/CD3-PC5, and total lymphocyte percentage when using CD45-FITC/CD56-RD1/ CD19-ECD/CD3-PC5. (fda.gov)
  • It would be vitally important to measure decrease or increase in the percentage and number of these important lymphocytes. (lymphocytemap.com)
Monocytes1
Cytoplasm1
  • [ 4 . 5 ] Adding to the challenge, classic lymphoglandular bodies, representing remnants of lymphocyte cytoplasm, are typically inconspicuous or absent in effusion fluids, in contrast to FNA material from solid specimens. (cytojournal.com)
Enumeration2
  • Enumeration of CD4+ lymphocytes in HIV-positive participants and age-matched controls was performed on cryopreserved whole blood using the method reported by Fiebig et. (cdc.gov)
  • Allows simultaneous identification and enumeration of total CD3+, total CD4+, total CD8+, dual CD3+/CD4+, dual CD3+/CD8+ and/or total CD3+, CD19+ and CD3+/CD56+ lymphocyte percentages and absolute counts in whole blood by flow cytometry. (fda.gov)
Lymphoma1
Leukemias1
  • Necessity for Automation ⚫Cell counts ⚫Dx of Hemoglobinopathies ⚫Immunophenotyping ⚫Dx of Leukemias & Lymphomas ⚫Coagulation Abnormalities. (slideshare.net)
Clinical1
  • Progressive depletion of CD4+ T-lymphocytes is associated with an increased likelihood of clinical complications. (cdc.gov)
Immune5
Gastrointestinal1
  • We compared the backgrounds of the samples between the groups to reveal crucial elements in the successful isolation of lymphocytes residing in the gastrointestinal tract. (bvsalud.org)
Counts1
  • Sometimes CLL is discovered accidentally when blood counts ordered for some other reason show an increased number of lymphocytes. (merckmanuals.com)
Isolation1
  • Comparison between the groups revealed lymphocyte isolation success rates differed between biopsy sites. (bvsalud.org)
Cell3
  • Here we describe genetic modification of antigen-presenting cell lines to facilitate the production of CD4(+) and CD8(+) T lymphocytes specific for CMV, EBV and several serotypes of adenovirus from a single cell culture. (nih.gov)
  • The Cambridge Biotech HIV-1 Western Blot Kit is manufactured by Calypte Corporation from HIV-I propagated in an H9/HTLV-IIIb T-lymphocyte cell line. (cdc.gov)
  • Correction for erythroid cell contamination in microassay for immunophenotyping of neonatal lymphocytes. (bdbiosciences.com)
Blood1
  • There is a significant difference between lymphocyte immunophenotyping and the complete blood count (CBC), which is used for checking the overall health of a patient. (lymphocytemap.com)
Monoclonal1
  • Immunophenotyping relies on detecting specific antigenic determinants on the surface of WBCs by antigen-specific monoclonal antibodies that have been labeled with a fluorescent dye or fluorochrome, such as phycoerythrin (PE) or fluorescein isothiocyanate (FITC). (cdc.gov)
Quality assurance1
  • To assure the accuracy and reliability of CD4+ T-lymphocyte test results obtained within individual laboratories and to assure the comparability of results between laboratories, standard methods for performing the test, as well as guidelines for quality control and quality assurance, are desirable. (cdc.gov)
AIDS2
  • The pathogenesis of acquired immunodeficiency syndrome (AIDS) is largely attributable to the decrease in T-lymphocytes bearing the CD4 receptor (CD4+) (1-5). (cdc.gov)
  • Moreover, CD4+ T-lymphocyte levels are being considered for inclusion as a criterion for classifying HIV-infected persons by the newly revised CDC HIV classification system and the proposed AIDS surveillance case definition for adults and adolescents. (cdc.gov)
Patients3
  • CD4+ T-lymphocyte levels are also used as prognostic indicators in patients with human immunodeficiency virus (HIV) disease (8). (cdc.gov)
  • The composition of lymphocytes in the gastric mucosa following the eradication of Helicobacter pylori (H. pylori) in patients with and without gastric cancer has not been compared. (bvsalud.org)
  • This study performed a single spot analysis of gastric mucosal lymphocytes after H. pylori eradication in patients with (n = 13) and without (n = 20) gastric cancer. (bvsalud.org)
Findings1
  • Together, these findings suggest dysregulated cytotoxic lymphocyte response to SARS-Cov-2 infection in MIS-C. (mit.edu)
Primary1
Infection1
Absolute1
Specific2
  • When administered to immunocompromised individuals, the single T lymphocyte line expands into multiple discrete virus-specific populations that supply clinically measurable antiviral activity. (nih.gov)
  • However, in most cases there are specific features that may aid in differentiating small reactive from non-reactive lymphocytes including the round shape of the nucleus, the absence of visible nucleoli and the presence of fine clumped chromatin. (cytojournal.com)