• microsatellite markers are available for the species (Bro wn et al. (researchgate.net)
  • We used next generation sequencing (Roche 454 pyrosequencing) to identify microsatellite markers from the genomic DNA of M. gilvus. (eurekamag.com)
  • This study also has effectively demonstrated the use of 454 pyrosequencing for the rapid development of informative microsatellite markers from a termite genome. (eurekamag.com)
  • Microsatellite DNA loci are genetic markers in wide use by Schistosoma researchers, but there are few primers available for S. haematobium . (biomedcentral.com)
  • About half of the primers in the database of Schistosoma haematobium microsatellite DNA loci should yield amplifiable and easily scored polymorphic markers, thus providing thousands of potential markers. (biomedcentral.com)
  • This paper describes the characterization of a set of nine microsatellite markers for the pipe-organ mud-dauber wasp, Trypoxylon albitarse F. (Hymenoptera: Crabronidae), a common solitary wasp species in Brazil. (unesp.br)
  • Significant differences in clinicopathologic characteristics and long-term prognoses were observed among patients with microsatellite-stable tumors, tumors having instability at 2 to 4 markers, and tumors having instability at all 5 markers (MSI/5). (univr.it)
  • The increasing availability of DNA amplification by PCR at the beginning of the 1990s triggered a large number of studies using the amplification of microsatellites as genetic markers for forensic medicine, for paternity testing, and for positional cloning to find the gene underlying a trait or disease. (wikipedia.org)
  • 19 individuals with ocular hypertension or glaucoma suspicion) were genotyped using 35 microsatellite markers on the candidate loci and analyzed for linkage. (nih.gov)
  • Analysis of markers on chromosome 19q gave a peak pairwise LOD score of 3.72 at D19S420, allowing assignment of a novel ADPHSP locus (which we have termed "SPG12") to this region. (nih.gov)
  • Haplotype construction and analysis of recombination events narrowed the SPG12 locus to a 16.1-cM region between markers D19S868 and D19S902. (nih.gov)
  • For the microsatellites, the ABI Prism Linkage Mapping Set version 2, with 402 microsatellite markers, was used, and, for the SNPs, the Early Access Affymetrix Mapping 10K array was used. (elsevierpure.com)
  • The greatest gain provided by the SNP markers was a large increase in the linkage information content, with an average information content of 61% for the SNPs, versus an average of 41% for the microsatellite markers. (elsevierpure.com)
  • The strengths and weaknesses of microsatellite versus SNP markers are illustrated by the results of our genome linkage scans. (elsevierpure.com)
  • Two microsatellite alleles at KIT-related markers were significantly associated (P (datadryad.org)
  • We employed, for the first time in this setting, real-time PCR with melting curve analysis in order to identify loss of heterozygosity (LOH) or microsatellite instability (MI) in two polymorphic markers (PYGM, D11S449) at the MEN1 locus and one additional marker (D11S906) of a putative oncosuppressive region distal to the MEN1 gene. (edgehill.ac.uk)
  • Population genetic diversity and species relationships in the genus Rhinanthus L. based on microsatellite markers, 495-502. (usd.edu)
  • In this study we have increased the density of microsatellite markers 2- to 5-fold in each of several major RI sets that share C57BL/6 as a parental strain (AXB, BXA, BXD, BXH, and CXB). (nervenet.org)
  • A set of 490 markers that were typed in all constituent RI sets were used as anchor loci to assemble high-resolution framework maps. (nervenet.org)
  • To follow up suggestive loci, an additional 13 families from other Nordic countries were genotyped for selected markers. (lu.se)
  • Methods: GWS was performed using 811 microsatellite markers providing about five centiMorgan (cM) resolution. (lu.se)
  • Eleven loci were found to be polymorphic with 2-4 alleles per locus. (eurekamag.com)
  • The number of alleles per locus ranged from two to 12, and observed heterozygosities from 0.00 to 0.865. (edu.au)
  • All loci were polymorphic with a number of alleles ranging from two to eight. (sharkyear.com)
  • Thirty-five alleles were detected in a sample of 45 individuals from 3 different populations, with an average of 3.9 alleles per locus. (geneticsmr.com)
  • Isolated loci contained allelic diversity of between 5 and 17 alleles. (australian.museum)
  • exhibit low levels of polymorphism in our samples (3.13 alleles per locus for eight successfully amplified loci) and 38% of loci tested present more than two alleles per primer set (data not shown). (bioone.org)
  • Zhang, De-Xing , Smith, Julie A. , Rico, Ciro and Hewitt, Godfrey M. (2001) Isolation and characterization of 10 microsatellite loci in poor cod Trisopterus minutus (L). Molecular Ecology Resources, 1 (1-2). (uea.ac.uk)
  • Isolation and characterization of 11 novel microsatellite loci in a West African leaf-nosed bat, Hipposideros aff. (edu.au)
  • Dive into the research topics of 'Isolation and characterization of 11 novel microsatellite loci in a West African leaf-nosed bat, Hipposideros aff. (edu.au)
  • Here we describe the isolation of eight polymorphic microsatellite loci using 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries. (sharkyear.com)
  • Isolation of microsatellite loci from endangered members of Lotus (Fabaceae) subgenus Syrmatium. (usd.edu)
  • Isolation of microsatellite loci from Rhamnus pirifolia, 471-474. (usd.edu)
  • Isolation of microsatellite loci from the endangered plant Galium catalinense subspecies acrispum (Rubiaceae). (usd.edu)
  • Isolation of microsatellite loci from the endangered plant Sibara filifolia (Brassicaceae). (usd.edu)
  • Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists and in genetic genealogy, or as simple sequence repeats (SSRs) by plant geneticists. (wikipedia.org)
  • Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. (wikipedia.org)
  • citation needed] Unlike point mutations, which affect only a single nucleotide, microsatellite mutations lead to the gain or loss of an entire repeat unit, and sometimes two or more repeats simultaneously. (wikipedia.org)
  • We assembled and aligned the S. haematobium sequences to the genomes of S. mansoni and S. japonicum , identifying microsatellite DNA loci across all three species and designing primers to amplify the loci in S. haematobium . (biomedcentral.com)
  • 13,790 primer pairs to amplify unique microsatellite loci in S. haematobium , (available at http://www.cebio.org/projetos/schistosoma-haematobium-genome ). (biomedcentral.com)
  • Primer sequences used to amplify 60 microsatellites used to adjust for population structure during association analyses. (datadryad.org)
  • Eight of the nine loci described were found to be polymorphic. (unesp.br)
  • Since population genetics and species-specific molecular tools remain unknown for all members of the Curimatidae family, this study developed a set of microsatellite loci and studied the population genetics of C. mivartii in the lower section of the Colombian Magdalena-Cauca basin. (peerj.com)
  • We have developed primers for nine polymorphic microsatellite loci to allow examination of intraspecific population subdivision associated with two aspects of the biology of their hosts: host species (oak gallwasps) and the tree on which the gallwasp develops (different oak species). (ed.ac.uk)
  • All nine loci amplified well across individuals collected from a range of gallwasp species and across two oak taxa. (ed.ac.uk)
  • The three Schistosoma genomes contained similar overall frequencies of microsatellites, but the frequency and length distributions of specific motifs differed among species. (biomedcentral.com)
  • The loci were robust, amplifying in other Eucalyptus species, Corymbia and Angophora. (edu.au)
  • Most eukaryotes have microsatellites, with the notable exception of some yeast species. (wikipedia.org)
  • We used the partial control region (CR) of mitochondrial DNA (mtDNA) and seven microsatellite loci to investigate the intra-species structure, differentiation, and demographic history of hog deer populations from three landscapes, the Terai Arc, Northeast, and Indo-Burma (Keibul Lamjao National Park (KLNP), Manipur, India) landscapes. (nature.com)
  • All nine primers were tested for cross-amplification in species from the Fabaceae-Mimosoidea family, yielding a transferability success rate of 7 loci in Stryphnodendron adstringens to 0 transferred loci in Pithecellobium incuriale and Inga marginata . (geneticsmr.com)
  • These microsatellites will be valuable to study population genetics of this and other species where primer transferability was detected. (geneticsmr.com)
  • To provide tools for conservation, ecological and behavioural studies, we isolated variable microsatellite repeat sequences and designed primers for PCR amplification in this species. (australian.museum)
  • Twelve new microsatellite sequences for the turkey were obtained from a small insert M13 library. (umn.edu)
  • We selected 20 microsatellite sequences identified from high throughput sequence reads and PCR amplified these for 38 individuals, yielding 11 consistently amplifying and scorable loci. (edu.au)
  • Results from nuclear and mtDNA sequences show that all Atlantic and Mediterranean populations belong to the same phylogroup, though microsatellite data indicate significant genetic divergence between the Azorean sample and all other locations, as well as among Macaronesian, western Iberian and Mediterranean regions. (nature.com)
  • SNP genotypes were obtained from HapMap and microsatellite genotypes from CEPH. (lancs.ac.uk)
  • We used genotypes at 11 microsatellite loci to reveal the genetic structure of, and directional gene flow among, populations to predict the likely future spread of the pathogen in the northwest and to estimate effective population size (Ne). (datadryad.org)
  • Microsatellites are also used in population genetics to measure levels of relatedness between subspecies, groups and individuals. (wikipedia.org)
  • However, identification of genetic susceptibility loci for prostate cancer has been extremely difficult. (elsevierpure.com)
  • A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from one to six or more base pairs) are repeated, typically 5-50 times. (wikipedia.org)
  • 1992). A microsatellite is a tract of tandemly repeated (i.e. adjacent) DNA motifs that range in length from one to six or up to ten nucleotides (the exact definition and delineation to the longer minisatellites varies from author to author), and are typically repeated 5-50 times. (wikipedia.org)
  • Similarly, insects have shorter repeat motifs in their telomeres that could arguably be considered microsatellites. (wikipedia.org)
  • We developed twelve E. globulus microsatellite loci for fingerprinting and future studies in genome mapping, gene flow and genetic diversity. (edu.au)
  • Microsatellites occur at thousands of locations within an organism's genome. (wikipedia.org)
  • Microsatellites are distributed throughout the genome. (wikipedia.org)
  • The human genome for example contains 50,000-100,000 dinucleotide microsatellites, and lesser numbers of tri-, tetra- and pentanucleotide microsatellites. (wikipedia.org)
  • In this study, we compare genome linkage scans using microsatellites with those using single-nucleotide polymorphisms (SNPs), performed in 467 men with prostate cancer from 167 families. (elsevierpure.com)
  • Recent genome-wide searches (GWS) have not revealed any single major locus reminiscent of BRCA1 and BRCA2, indicating that still unidentified genes may explain relatively few families each or interact in a way obscure to linkage analyses. (lu.se)
  • We wished to investigate the ability of different SNP chipsets to detect association with a disease and to investigate the linkage disequilibrium (LD) relationships between microsatellites and nearby SNPs in order to assess their potential usefulness to detect association. (lancs.ac.uk)
  • Additionally, LD relationships between 10 microsatellites and SNPs within 2Mb of each other were studied. (lancs.ac.uk)
  • Microsatellites had detectable LD with only a small number of nearby SNPs and the pattern of LD was very variable. (lancs.ac.uk)
  • Thirty-seven loci were selected for primer development and tested for polymorphism across 22 colonies of M. gilvus. (eurekamag.com)
  • Polymorphism at these new loci was examined in both domestic and wild birds. (umn.edu)
  • Other microsatellites are located in regulatory flanking or intronic regions of genes, or directly in codons of genes - microsatellite mutations in such cases can lead to phenotypic changes and diseases, notably in triplet expansion diseases such as fragile X syndrome and Huntington's disease. (wikipedia.org)
  • For example, the sequence TATATATATA is a dinucleotide microsatellite, and GTCGTCGTCGTCGTC is a trinucleotide microsatellite (with A being Adenine, G Guanine, C Cytosine, and T Thymine). (wikipedia.org)
  • The mutation rate at microsatellite loci depends on the repeat motif sequence, the number of repeated motif units and the purity of the canonical repeated sequence. (wikipedia.org)
  • This result indicated that the disruption of the mismatch repair system of Msh2 does not mainly lead to allelic loss of the FHIT/FRA3B locus as well as MSI in esophageal cancer. (elsevierpure.com)
  • Although the first microsatellite was characterised in 1984 at the University of Leicester by Weller, Jeffreys and colleagues as a polymorphic GGAT repeat in the human myoglobin gene, the term "microsatellite" was introduced later, in 1989, by Litt and Luty. (wikipedia.org)
  • Recombinant inbred (RI) strains have been used to map a wide range of Mendelian loci and quantitative traits (Taylor, 1989). (nervenet.org)
  • Primer pairs were developed and levels of diversity were assessed for eight microsatellite loci, including one locus linked to the gene encoding Microtubule-Associated Protein 2, a protein important for behavioural imprinting in birds, and one sex-linked locus. (australian.museum)
  • Microsatellite loci continue to be important tools in genetic analysis of populations and experimental families. (umn.edu)
  • The genetic structure was accessed by analyzing six microsatellite loci in 74 individuals of A. aestiva , including samples from the two subspecies ( A. a. aestiva and A. a. xanthopteryx ), from five populations: four in Brazil and one in Argentina. (geneticsmr.com)
  • Microsatellite loci evaluation indicated genetic similarity among isolates from the five patients as well as with previous malaria cases from Hispaniola. (cdc.gov)
  • Development and testing of microsatellite loci for the invasive herb Lupinus polyphyllus through 454 pyrosequencing. (utu.fi)
  • Our data indicate that real-time PCR with melting curve analysis is a reliable technique for LOH and MI detection and indicate that genetic errors at the MEN1 locus but also distal to it may be involved in the development of sporadic pulmonary carcinoid tumors. (edgehill.ac.uk)
  • Some of the eight loci developed here successfully cross-amplified in the bull shark Carcharhinus leucas (one locus), the spinner shark Carcharhinus brevipinna (four loci), the sandbar shark Carcharhinus plumbeus (five loci) and the scalloped hammerhead shark Sphyrna lewini (two loci). (sharkyear.com)
  • These microsatellite loci are potentially of value in the study of closely related economic pests such as seed predators of: almonds (E. amygdali) and pistachios (E. plotnikovi). (ed.ac.uk)
  • In this study, we examined genetic alterations at the MEN1 locus in archival material from 15 pulmonary carcinoids. (edgehill.ac.uk)
  • Haplotypes in nine affected family members mapped the loci to 2p23.2 to p21, 6q14.2 to q23.2 and 14q21.3 to q24.3. (lu.se)
  • Is the Subject Area "Genetic loci" applicable to this article? (plos.org)