• The present study investigated whether streptozotocin-diabetes could be reversed in FRG mice in which chimeric mouse-human livers can readily be established and, in addition, whether pancreatic transdifferentiation occurred in the engrafted human hepatocytes. (nih.gov)
  • Given that the survival of mice was remarkably decreased, hepatocyte de-differentiation could be correlated with the protection of liver tissue. (nii.ac.jp)
  • Moreover, after chronic-plus-binge ethanol feeding, hepatocyte-specific MANF knockout (HKO) mice displayed more severe hepatic steatosis and liver injury than wild-type (WT) control mice. (chinaphar.com)
  • In addition, HKO mice displayed unique urea cycle metabolite patterns in the liver with elevated ammonia accumulation after ethanol feeding. (chinaphar.com)
  • We examined how BAs initiate liver injury using isolated liver cells from humans and mice and in-vivo mouse models. (jci.org)
  • Chemokine induction by BAs was reduced in hepatocytes from Tlr9 -/- mice, while liver injury was diminished both in conventional and hepatocyte-specific Tlr9 -/- mice, confirming a role for Tlr9 in BA-induced liver injury. (jci.org)
  • In this study, we investigated the role of embryonic gene Cripto-1 (CR-1) in hepatocellular carcinoma (HCC) using hepatocyte-specific CR-1-overexpressing transgenic mice. (aging-us.com)
  • Hepatocyte-specific over-expression of CR-1 in transgenic mice enhanced hepatocyte proliferation after 2/3 partial hepatectomy (2/3 PHx). (aging-us.com)
  • However, histological signs of precancerous lesions, hepatocyte dysplasia or HCC formation were not observed in the livers of 3-, 6- or 8-month-old hepatocyte-specific CR-1-overexpressing transgenic mice. (aging-us.com)
  • These findings demonstrate that liver-specific CR-1 overexpression in transgenic mice deregulates signaling pathways and genes associated with HCC. (aging-us.com)
  • Metabolomics data derived from livers of PBS-injected control and B16F10 tumour-bearing mice (Supplementary Table 1 ) have been deposited at Figshare ( https://doi.org/10.6084/m9.figshare.22233187 ). (nature.com)
  • Metabolomics data derived from livers of PBS-injected control and K7M2 tumour-bearing mice (Supplementary Table 2 ) have been deposited at Figshare ( https://doi.org/10.6084/m9.figshare.22233253 ). (nature.com)
  • Lipidomics data derived from livers of PBS-injected control and B16F10 tumour-bearing mice (Supplementary Table 3 ) have been deposited at Figshare ( https://doi.org/10.6084/m9.figshare.22233265 ). (nature.com)
  • Lipidomics data derived from livers of PBS-injected control and K7M2 tumour-bearing mice (Supplementary Table 4 ) have been deposited at Figshare ( https://doi.org/10.6084/m9.figshare.22233274 ). (nature.com)
  • Hepatocytes from two-week-old mice were less susceptible to both t-BOOH and paracetamol toxicity than were those from adult mice. (nih.gov)
  • However, after inhibition of GSSG-Rd with BCNU, hepatocytes from these postnatal mice were more susceptible to paracetamol toxicity. (nih.gov)
  • This suggests that the higher activity of GSH-Px/GSSG-Rd in hepatocytes from two-week-old mice is responsible for their reduced susceptibility to paracetamol toxicity. (nih.gov)
  • Results/outcome In a cross-tissue metabolic flux study of 90-day-old mice using U13C-glucose as a stable tracer, we observe that the most robust metabolic changes are observed in the liver. (bmj.com)
  • Based on these in vivo results, we hypothesise that very early changes in fatty acid synthesis, mobilisation and/or catabolism are occuring in the liver of HttQ111/+ mice. (bmj.com)
  • To study this hypothesis in more mechanistic detail, we have established a system of primary hepatocytes isolated from 90 day old mice fed one of 3 diets from weaning as a lipid-relevant perturbagen: high fat (65% kcal/fat), medium fat (45% kcal/fat) and normal chow (10% kcal/fat). (bmj.com)
  • 2011 Mice lacking in MKK4 and MKK7 display defects in liver organ development because of loss of life and cell routine arrest of embryonic liver organ progenitors (Nishina ABT-492 et al. (healthanddietblog.info)
  • 2004 By dissecting the function of MKK7 and MKK4 in hepatocytes of adult mice Wuestefeld et al. (healthanddietblog.info)
  • Concurrent deletion of cyclin E1 and cyclin-dependent kinase 2 in hepatocytes inhibits DNA replication and liver regeneration in mice. (nature.com)
  • Hepatocyte-specific loss of GPS2 in mice reduces non-alcoholic steatohepatitis via activation of PPARα. (ki.se)
  • Absent expansion of AXIN2+ hepatocytes and altered physiology in Axin2CreERT2 mice challenges the role of pericentral hepatocytes in homeostatic liver regeneration. (amedeo.com)
  • Removal of two-thirds of the liver in mice causes the remaining liver tissue to regrow to its original size and restore function within 7-10 days . (medicalnewstoday.com)
  • Acetaminophen therapy of the mice resulted in over 30-fold growth of transgene-bearing hepatocytes and achieved therapeutic thresholds in hemophilia B and phenylketonuria. (nerc-bess.net)
  • Mice with hepatocyte -specific ADK disruption or overexpression were examined for hepatic fat deposition and inflammation . (bvsalud.org)
  • Whereas patients with nonalcoholic fatty liver disease had increased hepatic ADK levels, mice with hepatocyte -specific ADK disruption displayed decreased hepatic fat deposition on a chow diet and were protected from diet -induced excessive hepatic fat deposition and inflammation . (bvsalud.org)
  • In contrast, mice with hepatocyte -specific ADK overexpression displayed increased body weight and adiposity and elevated degrees of hepatic steatosis and inflammation compared with control mice . (bvsalud.org)
  • Furthermore, the early liver injury after CCl 4 treatment was significantly attenuated in hepatocyte-specific SSAT knockout mice (Hep-SSAT-Cko) compared with wild-type (WT) mice as determined by the reduced serum alanine aminotransferase levels, decreased hepatic lipid peroxidation, and less severe liver damage. (johnshopkins.edu)
  • In addition, Noxa and activated caspase 3 levels were elevated in the livers of WT compared with Hep-SSAT-Cko mice. (johnshopkins.edu)
  • 2005. Comparative hepatic toxicity: Prechronic/chronic liver toxicity in rodents. (nih.gov)
  • This study provides the first detailed catabolic profile of AZT at the hepatic site and emphasizes the critical role that the liver plays in drug clearance. (aspetjournals.org)
  • Hepatic steatosis plays a detrimental role in the onset and progression of alcohol-associated liver disease (ALD). (chinaphar.com)
  • At 1 week, transplanted cells were fully integrated in the liver parenchyma, along with expression of glucose-6-phosphatase and glycogen as reporters of hepatic function. (elsevierpure.com)
  • Our results reveal how tumour-derived EVPs dysregulate hepatic function and their targetable potential, alongside TNF inhibition, for preventing fatty liver formation and enhancing the efficacy of chemotherapy. (nature.com)
  • Although liver failure can be treated via hepatocyte transplantation, it also faces multiple problems comprising the shortage of high-quality hepatocytes sources, rejection of allogeneic transplants, difficulty to expand, and losing hepatic characteristics in vitro [ 7 , 8 ]. (springer.com)
  • Three hepatic veins remove blood from the liver. (medicalnewstoday.com)
  • Hepatocytes missing the important cofactor of Cyp enzymes, NADPH-cytochrome p450 reductase (Cypor), have been chosen in vivo by acetaminophen administration, changing as much as 50% of the hepatic mass. (nerc-bess.net)
  • Although PON1 affinity for the substrates was comparable in hepatic and enterocytic microsomes, the Vmax were 48- to 275-fold higher, in the liver. (cdc.gov)
  • Malaria transmission begins when uni-nucleated sporozoites are transmitted by mosquito bite, reach the liver and invade hepatocytes within which they transform into multi-nucleated hepatic schizonts. (elifesciences.org)
  • Cirrhosis is defined histologically as a diffuse hepatic process characterized by fibrosis and conversion of the normal liver architecture into structurally abnormal nodules. (medscape.com)
  • Common signs and symptoms may stem from decreased hepatic synthetic function (eg, coagulopathy), portal hypertension (eg, variceal bleeding), or decreased detoxification capabilities of the liver (eg, hepatic encephalopathy). (medscape.com)
  • Hepatic fibrosis is overly exuberant wound healing in which excessive connective tissue builds up in the liver. (msdmanuals.com)
  • Fibrosis itself causes no symptoms but can lead to portal hypertension (the scarring distorts blood flow through the liver) or cirrhosis (the scarring results in disruption of normal hepatic architecture and liver dysfunction). (msdmanuals.com)
  • Journal Article] Transplantation of Thy1+ cells accelerates liver regeneration by enhancing the growth of small hepatocyte-like progenitor cells via IL17RB signaling. (nii.ac.jp)
  • The only curative treatment for acute liver failure is liver transplantation, but there are many restrictions on the application of liver transplantation. (springer.com)
  • The aims of this article are to review the current knowledge regarding therapeutic mechanisms of mesenchymal stem cells in acute liver failure, to discuss recent advancements in preclinical and clinical studies in the treatment of mesenchymal stem cells, and to summarize the methodological improvement of mesenchymal stem cell transplantation in treating liver failure. (springer.com)
  • The only curative treatment for ALF is liver transplantation, but there are many restrictions on the application of liver transplantation because of financial considerations, a shortage of donor livers, and immunosuppression-related complications [ 3 ]. (springer.com)
  • Therefore, MSCs may help resolve issues restricting the application of hepatocyte transplantation. (springer.com)
  • We analyzed whether cytotoxic drugs interfering with the integrity of genomic DNA or cell division could be useful for liver cell transplantation. (elsevierpure.com)
  • Rats were pretreated with doxorubicin, irinotecan, or vincristine prior to cell transplantation and synergistic liver perturbations were induced by drug administration followed by partial hepatectomy or carbon tetrachloride treatments. (elsevierpure.com)
  • We conclude that the technique presented here allows isolation of large numbers of human hepatocytes from specimens unsuitable for transplantation but very valuable for biomedical research. (utmb.edu)
  • Conclusion: Functional HLCs were generated from human iPS cells, which could be used for autologus hepatocyte transplantation for liver failure and as in vitro model for determining the metabolic and toxicological properties ofdrug compounds. (elsevierpure.com)
  • Thus, rarely, significant symptoms or quality-of-life issues warrant consideration of liver transplantation. (msdmanuals.com)
  • [ 6 ] The notion of cure of hemophilia A by liver transplantation in human and canine subjects supports this statement. (medscape.com)
  • [ 8 ] In a mouse model, isolated hepatocytes transplantation did not correct hemophilia A, but transplantation of a cellular fraction enriched in liver endothelial cells did. (medscape.com)
  • As the root mechanism the writers recognize compensatory activation of MKK7 utilizing a combinatorial gene knockdown technique: Concurrent knockdown of MKK7 in MKK4-deficient hepatocytes abolishes the phenotype of accelerated proliferation. (healthanddietblog.info)
  • AIMS: The generation and characterisation of novel human hepatocyte lines (HHLs) derived from healthy human liver, retaining the primary hepatocyte phenotype. (ox.ac.uk)
  • All HHLs contained markers of hepatocyte and biliary phenotype (cytokeratins 7, 8, 18 and 19), Cytochrome P450 and albumin. (ox.ac.uk)
  • CONCLUSIONS: These HHLs retain primary hepatocyte phenotype and should be useful for investigating mechanisms of entry and replication of hepatotropic viruses, and should also be valuable in the study of hepatocyte biology and pathology. (ox.ac.uk)
  • Under conditions of over-nutrition this balance is disrupted, which promotes lipid accumulation in the hepatocytes to form a pathological phenotype of steatosis. (ki.se)
  • Results: The generated HLCs showed microscopically morphological phenotype of human hepatocytes, expressed liverspecific genes (ASGPR, Albumin, AFP, Sox17, Fox A2), secreted human liver-specific proteins such as albumin, synthesized urea and metabolized ammonia. (elsevierpure.com)
  • ON the other hand, Grhl2 null cholangiocytes isolated from chronically injured liver differentiated into hepatocytes in vitro. (nii.ac.jp)
  • Journal Article] Identification and In Vitro Expansion of Adult Hepatocyte Progenitors from Chronically Injured Livers. (nii.ac.jp)
  • Hepatocyte-specific MANF knockout led to decreased ASS1 activity, whereas overexpression of MANF contributed to enhanced ASS1 activity in vitro. (chinaphar.com)
  • Previous studies have proven that rodent and human MSCs can differentiate into HLCs in vitro and in vivo , which is one of the mechanisms of MSCs in the treatment of liver failure. (springer.com)
  • Methods: The following parameters in rat hepatocytes were investigated by using fluorescence probes in vitro and ex vivo: (1) mitochondrial membrane potential and membrane permeability transition, (2) oxygen radicals generation, (3) membrane barrier function, and (4) glutathione level. (elsevierpure.com)
  • Hydrogen peroxide was used to induce an oxidative stress mannequin of hepatocyte IAR-20 cells to guage the protecting results of BMMSCs in vitro. (nerc-bess.net)
  • Immunofluorescence analysis of hepatocytes infected with relapsing malaria parasites, in vitro ( P. cynomolgi ) and in vivo ( P. vivax ), reveals that LISP2 expression discriminates between dormant hypnozoites and early developing parasites. (elifesciences.org)
  • Quantitative RT-PCR showed expression of human and mouse endocrine hormones and β-cell transcription factors, indicating partial pancreatic transdifferentiation of mouse and human hepatocytes. (nih.gov)
  • At pathophysiologic concentrations, BAs induced proinflammatory cytokine expression in mouse and human hepatocytes, but not in nonparenchymal cells or cholangiocytes. (jci.org)
  • The paper establishes the relationship between enzymatic activity and the proliferation of hepatocytes. (peertechzpublications.com)
  • Low-intensity physical activity is shown not to influence the activity of hepatocytes' enzymes and their proliferation. (peertechzpublications.com)
  • at the same time, the proliferation of hepatocytes is inhibited for a long time. (peertechzpublications.com)
  • The purpose of this work was to study the influence of physical work on the activity of hepatocytes' enzymes and their proliferation. (peertechzpublications.com)
  • Perturbations in specific liver cell compartments benefit transplanted cell engraftment and/or proliferation. (elsevierpure.com)
  • In the liver the hepatocyte mass is kept stable through a good balance between hepatocyte death and proliferation that's ABT-492 frequently lost upon acute or chronic liver injury. (healthanddietblog.info)
  • Inhibition of MKK4 provides multiple beneficial results: Initial it considerably accelerates hepatocyte proliferation both in the fumarylacetoacetate hydrolase-deficient mouse style of subacute liver organ failing and after persistent liver organ damage with carbon tetrachloride. (healthanddietblog.info)
  • 2011 Because MKK4 also activates p38 signaling which generally inhibits proliferation it might be interesting to delineate the contribution of p38 suppression towards the phenotypes seen in MKK4-lacking hepatocytes. (healthanddietblog.info)
  • Although MKK4 deletions can be found in liver tumor their data show that MKK4 deficiency alone is definitely insufficient to cause hepatocyte proliferation or liver cancer. (healthanddietblog.info)
  • In mouse hepatocytes, p38γ induces proliferation after partial hepatectomy by promoting the phosphorylation of retinoblastoma tumour suppressor protein at known CDK target residues. (nature.com)
  • Fig. 1: p38γ phosphorylates Rb and promotes liver proliferation after PHx. (nature.com)
  • Notably, tumour EVP education enhanced side effects of chemotherapy, including bone marrow suppression and cardiotoxicity, suggesting that metabolic reprogramming of the liver by tumour-derived EVPs may limit chemotherapy tolerance in patients with cancer. (nature.com)
  • Fig. 1: Remote primary tumours induce metabolic dysfunction in the liver. (nature.com)
  • Fig. 2: Tumour-derived EVPs induce liver metabolic dysfunction. (nature.com)
  • Conclusions We conclude that early transcriptional and metabolic flux changes occur in the livers of B6. (bmj.com)
  • Our research aims to understand how metabolic stress signals reprogram hepatocytes and immune cells to promote the progression of these diseases. (ki.se)
  • The metabolic pathways in the liver are tightly controlled to balance its anabolic and catabolic activities. (ki.se)
  • Monocytes and liver-resident macrophages are activated by stimuli from the microenvironment and communicate with metabolic tissues to affect disease progression. (ki.se)
  • PPARα and NCOR/SMRT corepressor network in liver metabolic regulation. (ki.se)
  • Liver lipidomics , hepatocyte RNA sequencing ( RNA-seq ), and single-cell RNA-seq for liver nonparenchymal cells were performed to analyze ADK regulation of hepatocyte metabolic responses and hepatocyte -nonparenchymal cells crosstalk. (bvsalud.org)
  • Similar CYP- and PON1-mediated metabolic profiles were demonstrated in microsomes from liver or isolated intestinal enterocytes. (cdc.gov)
  • In the context of a toxicity study, some treated animals may have a depressed appetite and will have decreased amounts of glycogen in the liver, in contrast to the concurrent controls. (nih.gov)
  • Assessment of the roles of P-glycoprotein and cytochrome P450 in triptolide-induced liver toxicity in sandwich-cultured rat hepatocyte model. (curehunter.com)
  • The role of the glutathione peroxidase/reductase (GSH-Px/GSSG-Rd) enzyme system in protection from paracetamol toxicity was investigated in isolated mouse hepatocytes in primary culture. (nih.gov)
  • However, they also increased the susceptibility of hepatocytes to paracetamol toxicity, indicating that a component of paracetamol's toxic effect involves formation of species that are detoxified by the GSH-Px/GSSG-Rd enzymes. (nih.gov)
  • Low concentrations on cellular targets impair drug therapeutic effects, whereas excessive hepatocyte concentrations may induce cellular toxicity. (aspetjournals.org)
  • Furthermore, we found that part of hepatocyte express SOX9 during formation of hepatocellular carcinoma induced by diethylnitrosamine. (nii.ac.jp)
  • Acute liver failure is a life-threatening clinical syndrome characterized by rapid development of hepatocellular necrosis leading to high mortality and resource costs. (springer.com)
  • Acute liver failure (ALF) is a life-threatening clinical syndrome characterized by rapid hepatocellular necrosis due to various acute injuries induced by hepatotoxic drugs, immune-mediated attack, or viral infections. (springer.com)
  • The Hep G2 cell line has been isolated from a liver biopsy of a male Caucasian aged 15 years, with a well differentiated hepatocellular carcinoma. (sigmaaldrich.com)
  • 2013) performed an RNAi display screen in mouse types of subacute and chronic liver organ damage knocking down 301 putative tumor suppressor genes previously discovered in individual hepatocellular carcinomas. (healthanddietblog.info)
  • People with AATD are predisposed to obstructive pulmonary disease and liver disease (eg, cirrhosis and hepatocellular carcinoma in children and adults). (medscape.com)
  • Cirrhosis increases the risk of developing a type of liver cancer called hepatocellular carcinoma. (medlineplus.gov)
  • It is a common cause of chronic liver disease and hepatocellular carcinoma in Japan, the United States and many European countries. (medscape.com)
  • [ 1 , 2 ] Chronic infection with HCV is a major cause of progressive liver damage and long-term sequelae such as cirrhosis and hepatocellular carcinoma. (medscape.com)
  • ABSTRACT Serum levels of glutathione reductase (GR), glutathione S-transferase- (GST-) and malondialdehyde (MDA) were determined to evaluate their use in diagnosing hepatocellular damage in 75 children with liver disease. (who.int)
  • Conclusions: Active oxidants generated during ethanol metabolism increase mitochondrial permeability transition and modulate mitochondrial energy synthesis in hepatocytes. (elsevierpure.com)
  • Conclusions The data we obtained suggest that toyocamycin attenuates hepatocyte lipogenesis and ameliorates NAFLD in vivo and may therefore be beneficial in the treatment of NAFLD in humans. (elsevierpure.com)
  • CONCLUSIONS BMMSCs mixed with NMP may cut back the extent of ROS and free Fe²⁺ in oxidative stress broken rat DCD donor livers, doubtlessly cut back the ferroptosis in hepatocytes, and restore each morphology and performance of DCD donor livers. (nerc-bess.net)
  • Because the liver is probably the major site of AZT catabolism, the metabolism and transmembrane distribution of AZT were evaluated in freshly isolated rat hepatocytes, a model for the study at the cellular level of biosynthetic, catabolic, and transport phenomena in the liver. (aspetjournals.org)
  • So whether you are creating the next cure for an infectious disease such as hepatitis or malaria, building innovative gene therapies, targeting NASH, or testing your small molecule for metabolism or toxicology, with HepaCur™ Fresh hepatocytes you get a uniquely better option for answering your liver-specific questions. (yecuris.com)
  • Here we show that inflammation, fatty liver and dysregulated metabolism are hallmarks of systemically affected livers in mouse models and in patients with extrahepatic metastasis. (nature.com)
  • Fig. 5: Tumour EVPs suppress liver drug metabolism and enhance chemotoxicity. (nature.com)
  • Liver is the major organ of energy metabolism. (ki.se)
  • It is generally accepted that dysregulated liver lipid metabolism and inflammation are two crucial mechanisms involved in NAFLD development, although the transcription network and the related epigenome remodeling events remain largely unclarified. (ki.se)
  • We examined developmental variations between fetal/toddler and grownup livers, and used human induced pluripotent stem cell-derived hepatocyte-like cells (iHLC) to realize insights into the contribution of improvement to altered sterol metabolism and PNAC. (nerc-bess.net)
  • bile acid and lipid metabolizing processes within the immature human liver, and assist using iHLC as a related mannequin system of creating liver to review lipid metabolism and PNAC. (nerc-bess.net)
  • PON1-mediated metabolism of CPF- and DZN-oxon was also demonstrated in liver and enterocyte microsomes. (cdc.gov)
  • WANG, H. Histological changes, lipid metabolism and oxidative stress in the liver of Bufo gargarizans exposed to cadmium concentrations. (scielo.br)
  • The most favorable is moderate load, which leads to the activation of oxidative-reducing enzymes and increases the number of binucleated hepatocytes, which persist for a long time after the end of the experiment. (peertechzpublications.com)
  • Background and Aims: The precise pathogenic significance of oxidative injury in the evolution of alcohol-induced liver disease is still obscure. (elsevierpure.com)
  • Results: Ethanol (50 mmol/l) increased oxidative stress in hepatocytes and subsequently induced an increased mitochondrial permeability transition and a decreased membrane potential. (elsevierpure.com)
  • A continuous infusion of ethanol (50 mmol/l) increased oxidative stress and decreased mitochondrial membrane potential in the pericentral area of isolated perfused rat liver. (elsevierpure.com)
  • RESULTS Livers handled with NMP mixed with BMMSCs confirmed higher liver operate, relieved histopathological injury, lowered oxidative stress harm and ferroptosis, and the mechanism of discount was related to downregulation of intracellular reactive oxygen species (ROS) and free Fe²⁺ ranges. (nerc-bess.net)
  • Livers treated with NMP combined with BMMSCs showed better liver function, relieved histopathological damage, reduced oxidative stress injury and ferroptosis, and the mechanism of reduction was associated with downregulation of intracellular reactive oxygen species (ROS) and free Fe²⁺ levels. (medscimonit.com)
  • The yield of isolated normal viable hepatocytes is inversely proportional to the donor age. (utmb.edu)
  • BACKGROUND To enhance the standard of liver grafts from extended-criteria donors donated after circulatory dying (DCD), this examine explored whether or not bone marrow mesenchymal stem cells (BMMSCs) mixed with normothermic machine perfusion (NMP) have protecting results on DCD donor livers and the results of ferroptosis on this process. (nerc-bess.net)
  • MATERIAL AND METHODS Twenty-four male rat DCD donor livers have been randomly and averagely divided into regular, static chilly storage (SCS), NMP, and NMP mixed with BMMSCs teams. (nerc-bess.net)
  • Liver operate, bile secretion, and pathological options of DCD donor livers have been detected to guage the protecting results of NMP and BMMSCs on DCD donor livers. (nerc-bess.net)
  • BMMSCs confirmed vital protecting results on the ultrastructure of DCD donor livers and ROS-induced harm to IAR-20 cells underneath electron microscopy. (nerc-bess.net)
  • BMMSCs additionally considerably improved the expression stage of microtubule-associated protein 1 gentle chain 3 (LC3)-II in each DCD donor livers and ROS-induced injured IAR-20 cells, together with upregulating the expression of ferritin. (nerc-bess.net)
  • To improve the quality of liver grafts from extended-criteria donors donated after circulatory death (DCD), this study explored whether bone marrow mesenchymal stem cells (BMMSCs) combined with normothermic machine perfusion (NMP) have protective effects on DCD donor livers and the effects of ferroptosis in this procedure. (medscimonit.com)
  • Twenty-four male rat DCD donor livers were randomly and averagely divided into normal, static cold storage (SCS), NMP, and NMP combined with BMMSCs groups. (medscimonit.com)
  • Liver function, bile secretion, and pathological features of DCD donor livers were detected to evaluate the protective effects of NMP and BMMSCs on DCD donor livers. (medscimonit.com)
  • BMMSCs showed significant protective effects on the ultrastructure of DCD donor livers and ROS-induced injury to IAR-20 cells under electron microscopy. (medscimonit.com)
  • BMMSCs also significantly improved the expression level of microtubule-associated protein 1 light chain 3 (LC3)-II in both DCD donor livers and ROS-induced injured IAR-20 cells, including upregulating the expression of ferritin. (medscimonit.com)
  • We focus on the lineage plasticity of hepatocytes and cholangiocytes during development and regeneration. (nii.ac.jp)
  • Thus strategies for identifying druggable focuses on that could enhance liver regeneration are of great restorative value. (healthanddietblog.info)
  • shed brand-new light over the legislation and function of JNK signaling in postnatal liver organ regeneration and unexpectedly recommend inhibition of MKK4 as a technique for enhancing or rebuilding it. (healthanddietblog.info)
  • Because rodents typically feed at night, the degree of glycogen accumulation within hepatocytes is typically highest in the early morning hours and wanes throughout the day. (nih.gov)
  • The morphologic appearance of glycogen in hepatocytes is distinctive, allowing glycogen accumulation or depletion to be readily diagnosed in most cases. (nih.gov)
  • If, in contrast to the control animals, there is a treatment-related increase in glycogen in the hepatocytes, glycogen accumulation should be diagnosed in the treated animals and assigned a severity grade. (nih.gov)
  • Likewise, the accumulation of excess alpha1-antitrypsin in hepatocytes can also lead to destruction of these cells and ultimately, clinical liver disease. (medscape.com)
  • Transporter activities were assessed by concentration ratios between compartments and pharmacokinetic parameters that describe the accumulation and decay profiles of hepatocyte concentrations. (aspetjournals.org)
  • Nonlinear regression analysis of substrate accumulation in hepatocytes revealed that cellular efflux is measurable ∼4 minutes after the start of perfusion. (aspetjournals.org)
  • Repopulation of the cirrhotic liver with disease-resistant hepatocytes could offer novel therapies, as well as systems for biological studies. (elsevierpure.com)
  • Liver repopulation using disease-resistant hepatocytes will be applicable in chronic conditions, such as viral hepatitis or Wilson's disease. (elsevierpure.com)
  • Neither doxorubicin nor vincristine induced liver repopulation in animals up to three months, including after partial hepatectomy or carbon tetrachloride-induced additional liver injury. (elsevierpure.com)
  • with progression to the periportal hepatocytes during the day. (nih.gov)
  • Neutrophils in periportal areas of livers from cholestatic patients also correlated with elevations in their serum aminotransferases. (jci.org)
  • When hepatocytes were transplanted via the spleen, cells were distributed immediately in periportal areas, fibrous septa, and regenerative nodules of the cirrhotic liver. (elsevierpure.com)
  • The liver is one of the main organs that is that they are not distributed uniformly generates free radicals in different patho- throughout the liver, the periportal concen- logical conditions [ 1 ]. (who.int)
  • We previously reported that hepatobiliary transporter multidrug resistance-associated protein (MRP2/Mrp2) is considered to be the major cause of the interspecies differences detected by efflux of fluorescent substrates in isolated hepatocytes. (aspetjournals.org)
  • Methods Huh-7 cells and isolated rat primary hepatocytes were treated with palmitic acid (PA), which is a saturated FFA, in the presence or absence of toyocamycin. (elsevierpure.com)
  • Numerous treatment strategies for acute liver failure simply prevent complications and decelerate disease progression. (springer.com)
  • The progression of liver injury to cirrhosis may occur over several weeks to years. (medscape.com)
  • Catabolism of 3'-azido-3'-deoxythymidine in hepatocytes and liver microsomes, with evidence of formation of 3'-amino-3'-deoxythymidine, a highly toxic catabolite for human bone marrow cells. (aspetjournals.org)
  • Studies using rat and human liver microsomes demonstrated that the rate of formation of AMT and GAMT increased in the presence of NADPH, suggesting the involvement of a NADPH-dependent enzyme system. (aspetjournals.org)
  • Compared on a per nmol P450 basis, the Vmax for CPF in enterocytes was approximately 2-3 times higher than in liver microsomes for the production of CPF-oxon and TCP. (cdc.gov)
  • The smaller volume of intestine, lower amount of CYP, and higher Km for TCP in the enterocyte microsomes, resulted in a lower catalytic efficiency (2 and 62 times) than in liver for oxon and TCP. (cdc.gov)
  • Liver steatosis can further proceed to more severe stages of non-alcoholic steatohepatitis (NASH), fibrosis and cirrhosis, which can ultimately develop into liver cancer. (ki.se)
  • In this study, we examined the effect of toyocamycin on hepatocyte lipoapoptosis and steatosis. (elsevierpure.com)
  • Background Whether direct-acting anti-viral therapy can reduce liver fibrosis and steatosis in patients with chronic hepatitis C virus (HCV) infection is unclear. (medscape.com)
  • Aims To evaluate changes in liver stiffness and steatosis in patients with HCV who received direct-acting anti-viral therapy and achieved sustained virological response (SVR). (medscape.com)
  • Liver stiffness as evaluated by magnetic resonance elastography, steatosis as evaluated by magnetic resonance imaging-determined proton density fat fraction (PDFF), insulin resistance, and laboratory data were assessed before treatment (baseline) and at 24 weeks after the end of treatment (SVR24). (medscape.com)
  • Although most HCV-associated liver damage is immunomediated, [ 3 ] some histopathological features, such as liver steatosis, suggest a viral cytopathic effect. (medscape.com)
  • [ 6 ] The majority of patients with steatosis (78%) have mild steatosis, with less than 30% of hepatocytes affected. (medscape.com)
  • The assessment of the severity of liver fibrosis and steatosis is important when staging chronic HCV. (medscape.com)
  • [ 15-18 ] In addition, proton density fat fraction (PDFF) measurement is an MRI-based method for quantitatively assessing liver steatosis. (medscape.com)
  • We also observed fatty liver and decreased cytochrome P450 expression at diagnosis in tumour-free livers of patients with pancreatic cancer who later developed extrahepatic metastasis, highlighting the clinical relevance of our findings. (nature.com)
  • Non-alcoholic fatty liver disease (NAFLD) and cardiovascular diseases (CVD) are emerging as major threats to modern societies. (ki.se)
  • Background and aims A high serum level of saturated free fatty acids (FFAs) is associated with the development of nonalcoholic fatty liver disease (NAFLD). (elsevierpure.com)
  • Nonalcoholic fatty liver disease is highly associated with obesity and progresses to nonalcoholic steatohepatitis when the liver develops overt inflammatory damage. (bvsalud.org)
  • Finally MKK4 insufficiency also lowers the severe nature of liver organ fibrosis. (healthanddietblog.info)
  • Conversely, platelet count, which is inversely associated with liver fibrosis, increased significantly from baseline to SVR24. (medscape.com)
  • Many investigators have reported that interferon-based treatment is effective in reducing serum alanine aminotransferase (ALT) levels, eradicating HCV RNA and reducing liver fibrosis in patients with chronic HCV infection. (medscape.com)
  • Magnetic resonance elastography has been reported to be a useful method for diagnosing liver fibrosis in patients with chronic liver disease, even in the early stages. (medscape.com)
  • The livers were subjected to the standard histological procedure and 5μm sections were stained with hematoxylin-eosin. (scielo.br)
  • Under a magnification of 100X, this photomicrograph of a hematoxylin-eosin (H&E) stained liver tissue specimen, revealed the presence of histopathologic changes indicative of fatty degeneration. (cdc.gov)
  • In contrast, the time of cold organ preservation did not correlate with the viability or the yield of isolated hepatocytes. (utmb.edu)
  • In contrast, the three compartments contributed to the low liver concentrations obtained during BOPTA perfusion. (aspetjournals.org)
  • Lipidomic and single-cell RNA-seq analyses indicated that ADK-driven hepatocyte factors, due to mitochondrial dysfunction, enhanced macrophage proinflammatory activation in manners involving increased expression of stimulator of interferon genes . (bvsalud.org)
  • Data mining in liver disease patient cohorts allowed us to identify several candidate epigenomic modulators that are correlated with NAFLD phenotypes. (ki.se)
  • In the in vivo study, toyocamycin ameliorated the liver injury caused by FFC-induced NAFLD. (elsevierpure.com)
  • We have previously shown that the introduction of furin-cleavable human insulin (INS-FUR) to the livers in several animal models of diabetes resulted in the reversal of diabetes and partial pancreatic transdifferentiation of liver cells. (nih.gov)
  • When hepatocytes were introduced with the intracellular domain of Notch 2 and Grhl2, they transdifferentiated into CK19(+) cholangiocyte-like cells after chronic injury. (nii.ac.jp)
  • Journal Article] Isolation of Bipotential Liver Progenitor Cells from Neonatal Mouse Liver. (nii.ac.jp)
  • Journal Article] Hepatocytic parental progenitor cells of rat small hepatocytes maintain a self-renewal capability after long-term culture. (nii.ac.jp)
  • At 1 year, transplanted hepatocytes formed large clusters containing several-fold more cells than normal control animals, which was in agreement with increased cell turnover in the cirrhotic rat liver. (elsevierpure.com)
  • In recent years, a growing number of studies have shown that stem cells can effectively treat acute liver failure. (springer.com)
  • MSCs have the capacity of multiple differentiations and self-renewal and have been proven to be able to differentiate into a series of cell lineages, such as adipocytes, osteoblasts, chondroblasts, and hepatocyte-like cells (HLCs). (springer.com)
  • Lobules are the functional units of the liver and consist of millions of cells called hepatocytes. (medicalnewstoday.com)
  • The liver or bone marrow stores iron released from hemoglobin, which makes the next generation of blood cells. (medicalnewstoday.com)
  • The liver stores iron from hemoglobin in the form of ferritin, ready to make new red blood cells. (medicalnewstoday.com)
  • These cells destroy disease-causing viruses, bacteria, or other microorganisms that might enter the liver through the gut. (medicalnewstoday.com)
  • Objective: To generate a homogeneous population of patient-specific hepatocyte-like cells (HLCs) from humaniPS cells those show the morphologic and phenotypic properties of primary human hepatocytes. (elsevierpure.com)
  • Melanomacrophage centers (MMCs) consist of macrophages, such as phagocytic cells and fragments, mainly erythrocytes and pigments, such as melanin, hemosiderin and lipofuscin, located in the reticuloendothelial liver tissue, kidney and pancreas ( Agius and Roberts, 2003 AGIUS, C. (scielo.br)
  • Later in the course of the condition, iron also accumulates in liver cells (hepatocytes). (medlineplus.gov)
  • possibly because the iron that accumulates in the liver, bone marrow, and spleen is less available for production of red blood cells. (medlineplus.gov)
  • Ferroportin also transports iron out of reticuloendothelial cells in the liver, spleen, and bone marrow. (medlineplus.gov)
  • [ 5 ] Endothelial cells of liver are the major site of biosynthesis. (medscape.com)
  • Cells have liver function tests and liver ultrasound and 2 antioxidant mechanisms for ensuring a biopsy. (who.int)
  • While the human liver microsomal system demonstrated rapid clearance by CYP enzymes, the hepatocyte incubations showed much slower clearance, possibly providing some insight into the long duration of carfentanils effects. (diva-portal.org)
  • Transcriptomic analysis of matched samples with RNASeq reveals that the liver has more genotype-sensitive transcripts at this stage than does the striatum, the most vulnerable tissue in HD. (bmj.com)
  • This ligament is a band of tissue that keeps the liver anchored to the diaphragm. (medicalnewstoday.com)
  • A layer of fibrous tissue called Glisson's capsule covers the outside of the liver. (medicalnewstoday.com)
  • Out of 29 liver tissue sections examined, 14 had a low level of expression of insulin receptor type 1 by immunohistochemical studies. (who.int)
  • Pharmacokinetic modeling is applied to analyze liver images and calculate clearances mediated by hepatocyte transporters. (aspetjournals.org)
  • This liver-specific inflammatory response required BA entry into hepatocytes via basolateral transporter Ntcp. (jci.org)
  • Engraftment of human hepatocytes was confirmed by measuring human albumin levels. (nih.gov)
  • Immunofluorescence microscopy revealed the expression of human albumin and insulin in transduced liver samples. (nih.gov)
  • Nonfasting human C-peptide levels were significantly higher than mouse levels, suggesting that transdifferentiated human hepatocytes made a significant contribution to the reversal of diabetes. (nih.gov)
  • These data show that human hepatocytes can be induced to undergo partial pancreatic transdifferentiation in vivo, indicating that the technology holds promise for the treatment of type 1 diabetes. (nih.gov)
  • We incubated carfentanil with human hepatocytes for up to 1 h and analyzed samples on a Sciex 3200 QTRAP mass spectrometer to measure parent compound depletion and extrapolated that to represent intrinsic clearance. (diva-portal.org)
  • Pooled primary human hepatocytes were then incubated with carfentanil up to 6 h and analyzed for metabolite identification on a Sciex 5600+ TripleTOF (QTOF) high-resolution mass spectrometer. (diva-portal.org)
  • RESULTS: Primary hepatocytes were immortalised with Moloney's mouse leukaemia virus expressing E6 and E7 proteins of human papillomavirus, and cultures propagated long-term. (ox.ac.uk)
  • Large numbers of human hepatocytes were obtained from split and whole livers by using an adaptive form of the collagenase perfusion technique employed in rodent and human biopsies. (utmb.edu)
  • This technique allowed for the isolation of human hepatocytes on a large scale (up to 18.5 × 10 9 in one case) from normal and diseased liver specimens. (utmb.edu)
  • We used RNA-sequencing and computational strategies to check gene expression patterns in human fetal/toddler livers, grownup liver, and iHLC. (nerc-bess.net)
  • We recognized distinct gene expression profiles between the human feta/toddler livers in comparison with grownup liver, and shut resemblance of iHLC to human creating livers. (nerc-bess.net)
  • Liver sections of human subjects were examined for ADK expression using immunohistochemistry . (bvsalud.org)
  • Mechanisms of bile acid-induced (BA-induced) liver injury in cholestasis are controversial, limiting development of new therapies. (jci.org)
  • Moreover, CR-1 mRNA and protein levels were significantly higher in HCC tissues than adjacent normal liver tissues. (aging-us.com)
  • It also significantly reduced PA-induced hepatocyte lipoapoptosis. (elsevierpure.com)
  • By using our newly developed liquid chromatography-tandem mass spectrometry quantification method, we were able to measure the absolute amount of MRP2/Mrp2 in liver tissues and isolated hepatocytes across species. (aspetjournals.org)
  • Freshly isolated hepatocytes conserved MRP2/Mrp2 protein levels that are comparable with those in the liver tissues. (aspetjournals.org)
  • This pattern differs from that seen in a similar iron overload disorder called hereditary hemochromatosis , in which the excess iron accumulates primarily in the hepatocytes. (medlineplus.gov)
  • MEB did not efflux back to sinusoids, whereas BOPTA basolateral efflux contributed to the decrease in hepatocyte concentrations. (aspetjournals.org)
  • Hepatocyte ADK functions to promote excessive fat deposition and liver inflammation through suppressing hepatocyte fatty acid oxidation and producing hepatocyte -derived proinflammatory mediators. (bvsalud.org)
  • In this study, we showed that receptor-type PTPϵ (PTPϵM) dephosphorylated IR in rat primary hepatocytes and tyrosines 972, 1158, 1162 and 1163 were primary targets of PTPϵM. (bioone.org)
  • It was also demonstrated that PTPϵM suppressed insulin-induced glycogen synthesis and inhibited insulin-induced suppression of phosphoenol pyruvate carboxykinase (PEPCK) expression in primary hepatocytes. (bioone.org)
  • BACKGROUND: Liver cell lines closely resembling primary hepatocyte are essential for research on hepatitis viruses and hepatocyte function. (ox.ac.uk)
  • In those situations, a diagnosis of hepatocyte cytoplasmic vacuolization is appropriate, along with a severity grade, but the morphology of the cytoplasmic changes in the hepatocytes should be thoroughly described in the pathology narrative. (nih.gov)
  • Liver-specific pRB loss results in ectopic cell cycle entry and aberrant ploidy. (nature.com)
  • In addition, it was noted that a short time between declared death and organ harvest (cross clamp time) results in higher viability of hepatocytes. (utmb.edu)
  • However, levels and effects for an individual drug are unpredictable and do not correlate well with the type of liver injury, its severity, or liver test results. (msdmanuals.com)
  • 2017. Comparative histology in the liver and spleen of three species of freshwater teleost. (scielo.br)