Lipopolysaccharide macrophages. Medical search. Frequent questions

Treatment of human alveolar macrophages (AMs) with the synthetic GC dexamethasone (Dex) did not alter the expression of TLRs −1, −4, and −6. (frontiersin.org)
Alveolar macrophages (AMs) are the tissue-resident macrophages in the lung alveolar space. (frontiersin.org)
Lipopolysaccharide (LPS)-stimulated monocyte derived macrophages (MDMs) and alveolar macrophages (AMs) were cultured with dexamethasone and/or SB706504. (ox.ac.uk)
In children, the description of developmental changes in immune receptor expression on alveolar macrophages (AM) has been limited by the small number of cells recovered by bronchoalveolar lavage (BAL). (ersjournals.com)
It is concluded that laser scanning cytometry is suited to the study of immune receptor expression from small numbers of paediatric alveolar macrophages, when CD68 is used for cell identification. (ersjournals.com)
We show that metformin inhibited NLRP3 inflammasome activation and interleukin (IL)-1β production in cultured and alveolar macrophages along with inflammasome-independent IL-6 secretion, thus attenuating lipopolysaccharide (LPS)- and SARS-CoV-2-induced ARDS. (lu.se)

It has been generally accepted that M1 macrophages are polarised in an inflammatory environment to produce pro-inflammatory cytokines, whilst M2 macrophages are involved in anti-inflammation and aid tissue repair in wound healing. (nih.gov)
Macrophages were used because of their role in generation and secretion of multiple pro-inflammatory cytokines during inflammation, including the ones evaluated here. (emerging-researchers.org)
3 Activated macrophages contribute to inflammatory responses and can produce and release several cytokines into the general circulation to exert systemic effects that occur during sepsis. (asahq.org)
6 Cyclooxygenase 2 (COX-2) or inducible nitric oxide synthase (iNOS) is induced in macrophages by proinflammatory stimuli, including mitogens, cytokines, and bacterial lipopolysaccharide. (asahq.org)
For example, previous studies show that cannabinoids can work as anti-inflammatory drugs, they can suppress the signaling of T cell receptors, induce apoptosis, lower the levels of pro-inflammatory cytokines, and act on macrophages. (news-medical.net)
Macrophages play a significant role in many processes, such as the human immune function, parasite infection, and tissue remodeling by secreting cytokines and producing reactive oxygen and nitrogen intermediates. (frontiersin.org)
Prebiotic-treated mice exhibited a lower plasma lipopolysaccharide (LPS) and cytokines, and a decreased hepatic expression of inflammatory and oxidative stress markers. (bmj.com)
At infection sites, activated T cells secrete cytokines (eg, interferon-gamma [IFN-gamma]) that induce production of macrophage migration inhibitory factor, preventing macrophages from leaving. (msdmanuals.com)
M1, "classically activated," pro-inflammatory macrophages are stimulated by cytokines such as IFN-gamma and by various microbial components (eg, lipopolysaccharide). (msdmanuals.com)
M2 "alternatively activated" anti-inflammatory macrophages are stimulated predominantly by cytokines such as IL-4 and IL-13. (msdmanuals.com)

Expression levels of key TCA cycle enzymes and the autophagy-related protein light chain 3b (LC3b) were determined in raw 264.7 cells treated with lipopolysaccharide (LPS) and metformin (Met). (nature.com)
First, we evaluated the biocompatibility of xerogel-derived NO permeable membranes modified with fluorinated functional groups by growing RAW 264.7 macrophages on them. (intechopen.com)
And we performed the AFM measurements to examine the morphology of RAW 264.7 macrophages on xerogel membrane. (intechopen.com)
Finally, we successfully detected NO release in RAW 264.7 macrophages, using a planar-type xerogel-derived NO sensor. (intechopen.com)

However, this secretion was not evident in M2 macrophages following stimulation by HKC. (nih.gov)
In LPS‑induced‑inflammatory response in RAW264.7 macrophage cells, PB strongly inhibited secretion of all pro‑inflammatory mediators test and increased the production of IL‑10 and blockade of NF‑κB. (phcogres.com)
We have studied the effects of the p38 MAPK inhibitor N-cyano-N'-(2-{[8-(2,6-difluorophenyl)-4-(4-fluoro-2-methylphenyl)-7-oxo-7,8-dihydropyrido[2,3-d]-pyrimidin-2-yl]amino}ethyl)guanidine (SB706504) and dexamethasone on COPD macrophage inflammatory gene expression and protein secretion. (ox.ac.uk)
We also performed cytokine profiling using a multiplex assay, and IL-1beta secretion was monitored using ELISA, in HMDMs primed or not with lipopolysaccharide (LPS). (cdc.gov)
Enhanced M1-polarized macrophages in obese synovium decreased growth arrest-specific 6 (GAS6) secretion, resulting in impaired macrophage efferocytosis in synovial ACs. (elifesciences.org)
GAS6 secretion is decreased during M1 macrophage polarization during obese-OA, leading to impaired macrophage efferocytosis in synovial apoptotic cells. (elifesciences.org)
To investigate matrix metalloproteinase (MMP) and their inhibitors tissue inhibitor matrix metalloproteinase (TIMP) gene expression and secretion during equine deep digital flexor tendon (DDFT) tenocyte and macrophage (undifferentiated, proinflammatory, and regulatory) co-culture. (avma.org)

Macrophages can be divided into classically activated inflammatory macrophages (M1) and alternatively activated anti-inflammatory macrophages (M2). (nih.gov)
In general, macrophages are a very dynamic and plastic cell type and can be divided into two main groups: classically activated macrophages (M1) and alternatively activated macrophages (M2). (wikipedia.org)
Surprisingly, Mregs resemble classically activated macrophages more than alternatively activated macrophages, due to higher biochemical similarity. (wikipedia.org)
Unlike classically activated macrophages, Mregs produce low levels of IL-12, which is important because IL-12 induces differentiation of naïve helper T cells to Th1 cells which produce high levels of IFNγ. (wikipedia.org)

Then, primary human monocyte-derived macrophages (HMDM) were exposed for 24 h of the various CBNs to assess cytotoxicity. (cdc.gov)

The first signal promotes the differentiation of monocytes to macrophages and the second signal promotes immunosuppressive functions. (wikipedia.org)
CD14 antigen is strongly expressed on monocytes, macrophages, and weakly on neutrophils. (beckman.com)
Monocytes in the circulation are precursors to tissue macrophages. (msdmanuals.com)
Monocytes migrate into tissues, where over approximately 8 hours, they develop into macrophages. (msdmanuals.com)

Coculture of macrophages with regulatory T cells (Tregs) caused differentiation of macrophages toward Mreg phenotype. (wikipedia.org)
Low AKR1B10 expression induced M2 macrophage polarization and promoted the malignant phenotype of GC. (portlandpress.com)

These results suggest that HMW adiponectin mediates potent anti-inflammatory activities in macrophages by inhibiting its Akt-C/EBPß signaling pathway, thereby suppressing IL-1 ß gene expression . (bvsalud.org)
These results indicated that the anti‑inflammatory effects of PB may be attributed to the downregulation of iNOS and COX‑2 through the suppression of NF‑κB signaling pathway in RAW264.7 macrophages. (phcogres.com)

Therefore, studying the correlation between inflammation and macrophage mitochondrial function could lead to a better understanding of the link between inflammation and diseases. (nature.com)

Using a macrophage cell line, RAW264.7 cells, the effect of midazolam on proinflammatory mediators and activation of mitogen-activated protein kinase was measured by Western blot. (asahq.org)
Suppression of Lipopolysaccharide-Induced IL-1ß Gene Expression by High-Molecular-Weight Adiponectin in RAW264.7 Macrophages. (bvsalud.org)

LPS) is a potent factor in infection, which induces M1 macrophages resulting in higher levels of iNOS, TNFα and IL-12p70 which dictate inflammatory T cell responses. (nih.gov)
Low AKR1B10 expression induces M2 macrophage polarization and promotes the malignant transformation of GC. (portlandpress.com)

Furthermore, midazolam suppressed phosphorylation of p38 mitogen-activated protein kinase and also inhibited lipopolysaccharide-induced superoxide production in macrophages. (asahq.org)
Inhibition of lipopolysaccharide-stimulated chronic obstructive pulmonary disease macrophage inflammatory gene expression by dexamethasone and the p38 mitogen-activated protein kinase inhibitor N-cyano-N'-(2-{[8-(2,6-difluorophenyl)-4-(4-fluoro-2-methylphenyl)-7-oxo-7,8-dihydropyrido[2,3-d] pyrimidin-2-yl]amino}ethyl)guanidine (SB706504). (ox.ac.uk)
p38 mitogen-activated protein kinase (MAPK) signaling is known to be increased in chronic obstructive pulmonary disease (COPD) macrophages. (ox.ac.uk)

To activate microglia/macrophages, a potent inflammation inducer lipopolysaccharide (LPS, 5 μg/5 μl) was microinjected into rat corpus callosum. (korea.ac.kr)
However, the various lactoferrin forms did effectively inhibit the pro-inflammatory response in macrophages that were activated with lipopolysaccharide with the most potent effect observed for apolactoferrin. (springer.com)

CD38, an ADP ribosyl cyclase, is a 45 kDa type II transmembrane protein having a short N-terminal cytoplasmic domain and a long C-terminal extracellular domain, expressed on the surface of various cells including macrophages, lymphocytes, and pancreatic β cells. (molcells.org)
The initial tissue reaction to infection is a focal, intensely suppurative necrosis consisting largely of accumulations of polymorphonuclear leukocytes, followed by invasion of macrophages, epithelioid cells, and lymphocytes. (cdc.gov)

The role of midazolam in activation of macrophages during sepsis is not known. (asahq.org)
1,2 Lipopolysaccharide, a gram-negative bacterial outer membrane component, has been implicated as a critical factor contributing to the pathogenesis of sepsis. (asahq.org)
Treatment with DSF-LF NPs showed remarkable therapeutic effects on lipopolysaccharide (LPS)-induced sepsis. (chinaphar.com)
The underlying mode of action of these DSF-LF-NPs may contribute to efficiently suppressing macrophage-mediated inflammatory responses and ameliorating the complications caused by sepsis and UC. (chinaphar.com)

Intra-articular injection of GAS6 restored the phagocytic capacity of macrophages, reduced the accumulation of local ACs, and decreased the levels of TUNEL and Caspase-3 positive cells, preserving cartilage thickness and preventing the progression of obesity-associated OA. (elifesciences.org)
Intra-articular injection of GAS6 restored the phagocytic capacity of macrophages, decreased synovial cell apoptosis, and prevented OA progression in obese-OA mice. (elifesciences.org)
Macrophages are phagocytic cells present in tissues throughout the body. (msdmanuals.com)

Macrophages are heterogeneous cell populations that are present in all tissues. (nih.gov)
In this study, the authors demonstrated that patients with obese-OA and mice with ApoE deficiency showed phenotypes of synovitis and enhanced macrophage infiltration in synovial tissues. (elifesciences.org)
Macrophages are phagocytes found in tissues and maintain tissue homeostasis, regulate inflammation, and play a significant role in host protection. (frontiersin.org)

To address this hypothesis, we used an in vitro model of mouse macrophage cells to assess whether the flavonoids chrysin, apigenin and/or baicalein suppress expression of the inflammatory genes IL-6, IL-1β and/or TNFα. (emerging-researchers.org)
This study aims to explore the energy metabolism of macrophages under either inflammatory or anti-inflammatory conditions by adding LPS and Met, respectively, using an in vitro and an in vivo model. (nature.com)

Toll-like receptors (TLRs) enable innate immune cells, such as macrophages, to recognize a wide variety of microbial ligands, thereby promoting inflammation. (frontiersin.org)
This can be explained by the constitutive expression of IL-35 receptors (gp130 and IL-12Rβ2) in M2 macrophages for cytokine consumption. (nih.gov)
We have investigated the effects of LPS and PMA on the expression of functional mannose receptors in rat bone marrow-derived macrophages. (aai.org)
These results suggest that LPS/PMA treatment of macrophages results in an inactivation of mannose receptors with no effect on receptor turnover or biosynthesis. (aai.org)
Mregs were first described after FcγR ligation by IgG complexes in the occurrence of pathogen-associated molecular patterns (e. g. lipopolysaccharide or lipoteichoic acid) acting through Toll-like receptors. (wikipedia.org)
They function as receptors for the complex of lipopolysaccharide (LPS) and LPS-binding protein. (bvsalud.org)

Induction of inflammation was achieved by treatment with lipopolysaccharide, a bacterial component used experimentally to study inflammation. (emerging-researchers.org)

M2a, mainly known as alternatively activated macrophages, are macrophages associated with tissue healing due to the production of components of extracellular matrix. (wikipedia.org)
Out of all macrophages, Mregs show the highest expression of MHC II molecules and co-stimulatory molecules (CD80/CD86), which differentiates them from the alternatively activated macrophages, which show a very low expression of these molecules. (wikipedia.org)
Mregs also differ from alternatively activated macrophages by producing high levels of nitric oxide and low arginase activity. (wikipedia.org)
Lastly, they differ in the expression of FIIZ1 (Resistin-like molecule alpha1) and YM1 which are differentiation markers present on alternatively activated macrophages. (wikipedia.org)

Pretreatment with human native HMW adiponectin inhibited lipopolysaccharide (LPS)-induced interleukin-1 ß ( IL-1 ß) gene expression , but not tumor necrosis factor (TNF)-α expression. (bvsalud.org)

In cerebral ischemic insults, activated inflammatory cells such as microglia and macrophages may be implicated in the pattern and degree of ischemic injury by producing various bioactive mediators. (korea.ac.kr)

Therefore, the main objective of this study was to elucidate how iron-depleted, iron-saturated, and manganese-saturated forms of lactoferrin regulate intestinal barrier function via interactions with epithelial cells and macrophages. (springer.com)
Lactoferrin that was not bound to its cognate receptor was able to bind and neutralize lipopolysaccharide. (springer.com)

In summary, our data show that sTLR2 and full-length TLR2 are released by macrophages under anti-inflammatory conditions, which may contribute to GC-induced immunosuppression. (frontiersin.org)

Protective immunity is conferred by antibody to lipopolysaccharide and T-cell-mediated macrophage activation triggered by protein antigens. (cdc.gov)

The lamina propria of the small intestine is the main site of the intestinal immune system, which contains a large number of macrophages, CD4 T cells, and dendritic cells. (frontiersin.org)

Furthermore, long-term lipopolysaccharide (LPS) exposure mimicking immunosuppression in the resolution phase of inflammation synergistically increased Dex-mediated TLR2 upregulation. (frontiersin.org)
In this study, we found that lipopolysaccharide (LPS) induced CD38 upregulation and its extracellular release in J774 macrophage cells. (molcells.org)

Results: Macrophage polarization was the main immune infiltration pattern in GC, and the state of macrophage polarization was closely related to the pathological grading of GC and the clinical stage of patients. (portlandpress.com)
Conclusion: M2 macrophage polarization is the main immune infiltration pattern in GC. (portlandpress.com)
The present study revealed that obese OA patients and Apoe −/− mice showed a more pronounced synovitis and enhanced macrophage infiltration in synovial tissue, accompanied by dominant M1 macrophage polarization. (elifesciences.org)
The number and diameter of lung vessels and macrophage infiltration were assessed using immunofluorescence staining. (spandidos-publications.com)

Lipopolysaccharide (LPS), the major outer membrane component in Gram-negative bacteria, is one of the most characterized PAMPs ( 10 ). (spandidos-publications.com)

Candida albicans induced a high level of EBI3 expression in M2 macrophages, which served as a mechanism for IL-12p70 suppression by competitive binding of the common protein subunit (p35) of IL-35 and IL-12p70. (nih.gov)
We conclude that SB706504 targets a subset of inflammatory macrophage genes and when used with dexamethasone causes effective suppression of these genes. (ox.ac.uk)

RT-PCR showed that LPS injection induced iNOS mRNA expression mostly in microglia/macrophages, peaking in intensity at 15 h after LPS injection. (korea.ac.kr)

by contrast, interleukin-4 (IL-4)-activated macrophages mainly use oxidative phosphorylation and fatty acid oxidation to generate energy. (nature.com)
Elevated levels of interleukin 6 (IL-6), granulocyte-macrophage colony-stimulating factor (GM-CSF), and granulocyte colony-stimulating factor (G-CSF) have been found in the serum of some patients. (medscape.com)

Carbon-based nanomaterial s trigger inflammasome activation in human macrophages: role of toll-like receptor activation and intracellular sensing via NLRP3. (cdc.gov)
Pathogenicity is related to production of lipopolysaccharides containing a poly N-formyl perosamine O chain, Cu-Zn superoxide dismutase, erythrulose phosphate dehydrogenase, stress-induced proteins related to intracellular survival, and adenine and guanine monophosphate inhibitors of phagocyte functions. (cdc.gov)
It is a facultative intracellular bacterium that multiplies within macrophages. (cdc.gov)

Unsensitized animals challenged with TMA exhibited also an increased number of macrophages in BAL, but gene expression in the above-mentioned gene pathways was unchanged or down-regulated. (cdc.gov)

Dermal sensitization (50% and 25% w/v) with TMA and an inhalation challenge of 15 mg/m3 TMA-induced apneas, laryngeal inflammation, increased numbers of eosinophils, neutrophils and macrophages in bronchoalveolar lavage (BAL), and increased immunoglobulin E levels in serum and lung tissue. (cdc.gov)

My contribution to the research included culturing mouse macrophage cells to be used for the experiment. (emerging-researchers.org)
I then stimulated the cells with lipopolysaccharide (LPS) to mimic inflammation, and then removed and replaced with flavonoids or vehicle control. (emerging-researchers.org)
Similar effect provoked interaction of macrophages and B1 B cells. (wikipedia.org)
with small or large lateral dimensions) are sensed by primary human macrophages which are key cells of the innate immune system. (cdc.gov)
The cells were then pretreated with CBD and DMH-CBD and then stimulated with lipopolysaccharide. (news-medical.net)
None of the tested forms induced a pro-inflammatory response in Caco-2 cells or in macrophages either. (springer.com)
Macrophages, which are functional plasticity cells, have the ability to phagocytize and digest foreign substances and acquire pro-(M1-like) or anti-inflammatory (M2-like) phenotypes according to their microenvironment. (frontiersin.org)
The large number of macrophages in the intestinal tract, play a significant role in maintaining the homeostasis of microorganisms on the surface of the intestinal mucosa and in the continuous renewal of intestinal epithelial cells. (frontiersin.org)
In addition, the effects of macrophages in the occurrence and development of inflammatory bowel disease (IBD), and their role in inducing fibrosis, activating T cells, reducing colitis, and treating intestinal inflammation were also reviewed in this paper. (frontiersin.org)
On the other hand, phagosomes are formed when the pathogen is engulfed by macrophages and fuse with lysosomes to release enzymes and toxic substances, resulting in killing or having cytotoxic effects on bacteria and tumor cells. (frontiersin.org)
This development occurs under the influence of macrophage colony-stimulating factor (M-CSF), which is secreted by various cell types (eg, endothelial cells, fibroblasts). (msdmanuals.com)

or 4) a fluorescent-labelled monoclonal antibody to CD68, a pan-macrophage antigen. (ersjournals.com)

Activation of the hypothalamic-pituitary-adrenal axis leads to production of glucocorticoids that cause decreased production of IL-12 by macrophages. (wikipedia.org)

Une différence statistiquement très importante a été observée entre les taux du facteur d'inhibition de la migration des macrophages des cas de septicémie et des témoins tandis qu'une différence statistiquement significative a été notée entre le taux MIF des différents génotypes des groupes étudiés. (who.int)
Le taux du facteur d'inhibition de la migration des macrophages était nettement associé à la mortalité dans les cas de septicémie. (who.int)

DSF-LF NPs could effectively block pyroptosis and inflammatory cytokine release from macrophages. (chinaphar.com)

6 - 9 The link between high-fat diet-induced inflammation, oxidative stress, metabolic disorders, and gut microbiota, could be lipopolysaccharide (LPS)-dependent. (bmj.com)
Oxidative stress and reactive oxygen species (ROS), which are majorly sourced from macrophages [ 5 ], may be the major contributory factors leading to testicular cell apoptosis and acute inflammation of testes [ 6 ]. (hindawi.com)

HCS) as well as small and large GOs, and CNTs, are capable of activating inflammasome, but only when the macrophages are LPS primed. (cdc.gov)

The interaction of GCs with macrophages in the immunosuppressive resolution phase upon prolonged TLR activation is widely unknown. (frontiersin.org)

In this study, we demonstrate that heat-killed C. albicans (HKC) strongly suppressed LPS-induced IL-12p70 production in M2 macrophages. (nih.gov)

In the present study, we provide the evidence that activated microglia/macrophages accelerate cerebral ischemic injury by overexpression of inducible nitric oxide synthase (iNOS). (korea.ac.kr)

A clear understanding of the function of macrophages, as well as their role in pathogens and inflammatory response, will delineate the next steps in the treatment of intestinal inflammatory diseases. (frontiersin.org)
In this review, we discuss the origin and development of macrophages and their role in the intestinal inflammatory response or infection. (frontiersin.org)

It functions as a high affinity receptor for the complex of LipoPolySaccharide (LPS) and the LPS-Binding Protein (LBP). (beckman.com)

While macrophage-induced inflammation is crucial for the response to pathogen infection, excessive or sustained inflammation can cause tissue damage and may lead to different conditions such as diabetes, cardiovascular diseases, and tumors 2 . (nature.com)

The present study found that synovial macrophages infiltrated and polarized in the obesity microenvironment and identified the essential role of M1 macrophages in impaired macrophage efferocytosis using pathology analysis of obesity-associated OA. (elifesciences.org)

In this study, we used lipopolysaccharide (LPS) and adenosine triphosphate (ATP) to simulate an inflammatory environment as the testing model. (spandidos-publications.com)

Down-regulation of mannose receptor activity in macrophages after treatment with lipopolysaccharide and phorbol esters. (aai.org)
Binding of 125I-mannose-BSA to intact macrophages showed a 70% decrease in surface receptor activity after treatment with LPS/PMA. (aai.org)
The measurement of developmental changes in alveolar macrophage (AM) immune receptor expression using flow cytometry (FC) in children is limited by several factors. (ersjournals.com)

The second occurs by influx of growth factors secreted by macrophages, platelets and fibroblasts, by fibroblast proliferation and subsequent synthesis and remodelling of collagenous dermal matrix. (worldwidewounds.com)

One of the key pioneering studies on macrophage metabolism. (nature.com)
An important early study on metabolism in different types of macrophages. (nature.com)

Adiponectin 's anti-inflammatory effects on macrophages remain controversial. (bvsalud.org)
Here, we investigated whether native HMW adiponectin from human plasma has anti-inflammatory effects on macrophages . (bvsalud.org)
They found that while the effect of CBD to reduce NF-kB activity was related to its effects on macrophage viability, the effects of DMH-CBD on Nf-kB were independent of its role in cell death. (news-medical.net)
The present study is aimed at investigating the anti-inflammatory, antioxidative, and antiapoptotic effects of methane on lipopolysaccharide- (LPS-) induced acute orchitis and its potential mechanisms. (hindawi.com)

The aim of this study was to evaluate the antiinflammatory actions of midazolam in cultured macrophages. (asahq.org)

The hypothesis was further verified in a lipopolysaccharide (LPS)‑induced lung injury mouse model. (spandidos-publications.com)

Anatomy32

Organisms29

Diseases9

Chemicals and Drugs116

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes49

Disciplines and Occupations1

Information Science4

Alveolar macrophages6

Cytokines10

264.74

Secretion7

Classically activated4

Monocyte derived macrophages1

Monocytes4

Phenotype2

Signaling pathway2

Inflammation and macrophage1

RAW264.72

Induces2

Mitogen-activa3

Potent2

Lymphocytes2

Sepsis4

Phagocytic3

Tissues3

Vitro2

Receptors6

Bacterial1

Alternatively activated4

Pretreatment1

Mediators1

Lactoferrin2

Anti-inflammatory conditions1

Antigens1

Dendritic1

Upregulation2

Infiltration4

Outer membrane component1

Suppression2

MRNA expression1

Interleukin2

Intracellular3

Gene1

Neutrophils1

Cells11

Antigen1

Glucocorticoids1

Migration2

Cytokine1

Oxidative2

Inflammasome1

Immunosuppressive1

Production1

INOS1

Intestinal2

Protein1

Pathogen1

Microenvironment1

Inflammatory environment1

Receptor3

Influx1

Metabolism2

Effects4

Study1

Lung1