• In this study, the authors used DNA microarray to compare and identify genes induced by HER2 in mammary epithelial cell line with ectopic HER2 overexpression and breast cancer cell lines derived from patients with different level of HER2 expression. (biomedcentral.com)
  • PI3K activity was thought to be the mediator of the HER2 control on FASN expression because LY294002, a known PI3K inhibitor, abrogated HER2 induced FASN protein production in the HER2 overexpressing normal mammary epithelial and breast cancer cells. (biomedcentral.com)
  • Here we investigated whether polarized monolayers of intestinal epithelial cells might regulate inflammatory responses by secreting IL-8 in a vectorial fashion (i.e. apical versus basolateral) depending on the location of the TLR stimulus. (biomedcentral.com)
  • The CXCR1 receptor for IL-8 was expressed only on the apical membrane of Caco-2 BBE cells and differentiated epithelial cells in the human small intestine and colon. (biomedcentral.com)
  • These results show that IL-8 induces autocrine signalling via an apical CXCR1 in Caco-2 BBE intestinal epithelial cells and that this receptor is also expressed on the apical surface of differentiated human intestinal epithelial cells in vivo , suggesting an autocrine function for IL-8 secreted in the lumen. (biomedcentral.com)
  • Intestinal epithelial cells (IECs) play a key role in the inflammatory response to colonizing or invading microorganisms via the secretion of interleukin-8 (IL-8). (biomedcentral.com)
  • Interestingly, IL-8 has been measured in the colon milieu of healthy volunteers using a dialysis bag inserted in the rectum [ 9 ] suggesting that it might be apically secreted by colonic epithelial cells. (biomedcentral.com)
  • Scanning electron microscopy displayed severe loss of normal array of concentric microridges, swelling of microridges, damage in epithelial cells and appearance of vacuoles on the stratified epithelium, while loss of regular pattern of microridges were observed under field condition. (omicsonline.org)
  • Methods: Primary mouse enteric neurons (EN) were exposed to a conditioned medium from ACE2-expressing Caco-2 colonic epithelial cells infected with SARS-CoV-2 or treated with tunicamycin (ER stress inducer). (emory.edu)
  • This research includes comparative studies of the behavior of liposomes and nanostructured lipid-carriers in a series of well-structured in vitro tests that could assess their interactions with the hCMEC / D3 and SH - SY5Y cell lines as an in vitro model of the blood-brain barrier and neuronal function, respectively. (mdpi.com)
  • Cadmium a very toxic and ubiquitous metal and ethanol (95%) are known to induce lipid peroxidation (Traore et al . (scialert.net)
  • Adenine has been suggested to induce cell cycle arrest in cancer cells, leading to cell death [ 9 , 10 ]. (hindawi.com)
  • We used a previously established model of mouse primary enteric neuronal cells and an enteric neuronal cell line treated with palmitate (PA) and lipopolysaccharide (LPS) to induce mitochondrial dysfunction and enteric neuronal cell death. (emory.edu)
  • The results clearly highlight the influence of the different fatty acids of the Gb 3 sphingolipids on the phase behaviour and the binding properties of Shiga toxin B subunits, even though the membranes were only doped with 5 mol% of the receptor lipid. (springer.com)
  • Transcriptome analyses revealed that Caco-2 BBE cells respond to stimulation with IL-8 supporting the hypothesis that IL-8 induces G protein-coupled receptor signalling. (biomedcentral.com)
  • IL-8 can signal through two receptors, CXCR1 and CXCR2 both of which are members of the G-protein-coupled receptor (GPCR) family [ 2 ]. (biomedcentral.com)
  • Additionally, we determined the localization of the CXCR1 receptor in Caco-2 BBE cells and human intestinal tissue samples and investigated the role of IL-8 autocrine signalling using transcriptomics. (biomedcentral.com)
  • The farnesoid X receptor (FXR) regulates the homeostasis of bile acids, lipids and glucose. (nature.com)
  • IL-2 and IFN-γ), immune checkpoint blockade (ICB) therapy (e.g., anti-PD-1/PD-L1 antibodies), and adoptive T-cell transfer (e.g., chimeric antigen receptor (CAR) T-cell therapy) [ 3 - 5 ]. (thno.org)
  • Being typically a minor component of the cell membrane, they are of utmost importance for biological functions that rely on lipid-lipid and lipid-protein interactions (Schnaar and Kinoshita 2015 ). (springer.com)
  • Furthermore, permeability was found to be highly temperature-dependent in Chinese hamster ovary (CHO) control cells and artificial membranes (parallel artificial membrane permeability assay). (aspetjournals.org)
  • Since only the enzyme isoform 2 seems to be responsive to membrane related signals ( e.g. myo-inositol), Sam2p was tagged with GFP to analyse its abundance and cellular localization under different stress conditions. (biomedcentral.com)
  • Western Blot: SR-BI Antibody [NB400-104] - Detection of SR-BI in rat H4IIE total cell lysates and plasma membrane proteins. (novusbio.com)
  • A-type lamin-dependent Caveolin-2 homo-oligomerization in the inner nuclear membrane microdomain is a precondition for pY19-Caveolin-2-mediated insulin-response epigenetic activation at the nuclear periphery. (cusabio.com)
  • Plays important role in modulating gene expression, cell membrane signaling, lipid transport and metabolism, and early brain development. (nutrimal.co)
  • 13]. In reality, phospholipids make up the outer cell membrane of all living things. (pdfplayer.org)
  • Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). (thermofisher.com)
  • Finally, C3R (100 μM) was able to suppress mRNA expression of NPC1L1 in Caco-2 cells after 24 h incubation. (biomedcentral.com)
  • CXCR1 is expressed in the human IEC line Caco-2 BBE [ 3 ], which is in agreement with our own microarray data using Caco-2 BBE cell mRNA (see below). (biomedcentral.com)
  • mRNA microarray analyses of A549, PC14, and PC14CDDP cells that were transiently transfected with either pre-miR-197 or LNA-miR-197 and their controls. (nih.gov)
  • The present study had evaluated the protective effect of hydroalcoholic (50-50: v/v) and aqueous extracts of L. kerstingii and M. oleifera against lipid peroxidation induced in vivo and in vitro by either cadmium or ethanol. (scialert.net)
  • Similar results were found in the kidney even though lipid peroxidation is slightly increased by ethanol in this organ. (scialert.net)
  • Free radicals are responsible for lipid peroxidation and have received much attention recently in connection with a variety of pathologies such as cancer (Zhu et al . (scialert.net)
  • Western diet (WD)-feeding increased hepatic lipid peroxidation in control mice and, in vitro palmitate induced oxidative stress and impaired the mitophagic clearance of damaged mitochondria in hepatocytes. (emory.edu)
  • All treatments reduced the adhesion ability of the bacteria and it is probably related to the metabolism cell reduction. (preprints.org)
  • Reduction of total cholesterols (TC) and LDL-C by dietary alterations and medications that affect lipid metabolism [ 14 ] is found to reduce the occurrence of atherosclerosis in animals and clinical cardiovascular events in human [ 15 ]. (hindawi.com)
  • Transmission electron microscopic study of gill showed severe damages which included degenerative changes in mitochondria, cellular vacuolation, damage in tubule vascular system, presence of lipid droplets, elongated nucleus, but in case of field experiment dilated mitochondria and cytoplasmic vacuolation were more prominent. (omicsonline.org)
  • Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. (thermofisher.com)
  • This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. (thermofisher.com)
  • A transcriptome analysis of HER2 in breast cancer cells has revealed a molecular connection between FASN and HER2 through the PI3K/Akt pathway [ 10 ]. (biomedcentral.com)
  • High-resolution single-cell transcriptome sequencing technology can visualize the heterogeneity of malignant and non-malignant cells in the tumor microenvironment in real time, especially combined with spatial transcriptome analysis, which can directly compare changes between different stages of metastatic samples. (bvsalud.org)
  • Breast cancer metastasis is a complex, multi-step process, with high cellular heterogeneity between primary and metastatic breast cancer, and more complex interactions between metastatic cancer cells and other cells in the tumor microenvironment. (bvsalud.org)
  • Therefore, this study takes single-cell analysis as the first perspective to deeply explore special or rare cell subpopulations related to breast cancer metastasis, systematically summarizes their functions, molecular features, and corresponding treatment strategies, which will contribute to accurately identify, understand, and target tumor metastasis-related driving events, provide a research basis for the mechanistic study of breast cancer metastasis, and provide new clues for its personalized precision treatment. (bvsalud.org)
  • Tumors/tumor Bearing Rats (TBR) were 3.8, 2 and 1.56 for C, RB 5% and RB 10%, respectively. (scialert.net)
  • Most of them generally follow the same pathway to make immune-activated cytotoxic T lymphocytes (CTLs) abundantly infiltrate into tumor tissues and destroy tumor cells [ 6 ]. (thno.org)
  • Of note, the TME is rich in immunosuppressive cells (e.g., tumor-associated macrophages (TAMs), regulatory T cells (Tregs), immature dendritic cells (iDCs) and myeloid-derived suppressor cells (MDSCs)), that can suppress antitumor immunity as a result of secretion of various cytokines and chemokines, which enables tumor escape from immune surveillance [ 8 ]. (thno.org)
  • Among most immunosuppressive cells, TAMs, as crucial drivers of immunosuppressive TME, account for the largest proportion of immune cells in the TME (approximately 50% of tumor mass) [ 9 , 10 ], which possess highly heterogeneity and play a complex regulatory role in tumor immunity and immunotherapy due to helping tumor evade immune surveillance [ 11 ]. (thno.org)
  • The level of cell polarization in cells grown on transwell permeable supports was characterized at multiple timepoints with four different methods: transepithelial electrical resistance (TEER) measurement, lucifer yellow permeability assay, alkaline phosphatase expression and ZO-1 expression. (helsinki.fi)
  • Due to the abundance of waste crustacean exoskeletons, extraction from crustaceans is the most common procedure, and two routes can be generally followed: biological, or chemical methods (see Figure 2 ). (mappingignorance.org)
  • Based on previous studies, PREP-inhibition seems to increase the amount of Beclin 1 and to affect the phosphorylation of Bcl-2 and Beclin 1, leading to dissociation of the complex. (helsinki.fi)
  • Synthesized peptide derived from Human Caveolin-2 around the phosphorylation site of Y27. (cusabio.com)
  • Lipid nano-systems stand out as one of the most attractive vehicles that can fuse with endothelial cells and transport active compounds. (mdpi.com)
  • However it remains unknown which endocytic pathways are active in brain endothelial cells. (helsinki.fi)
  • In present study we characterized human brain endothelial cells, hCMEC/D3, which are widely utilized as BBB in vitro model. (helsinki.fi)
  • As brain endothelial cells are polarized in vivo, the aim of the study was to demonstrate the cell polarization of hCMEC/D3 cells and to study the activity and functionality of different endocytic pathways as a function of cell polarization. (helsinki.fi)
  • The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. (cusabio.com)
  • In contrast to wild-type-Cav-2, retroviral re-expression of Y19/27F-Cav-2 in Cav-2 knockout endothelial cells did not affect anti-proliferative effect of TGF-beta compared to empty vector. (cusabio.com)
  • The migration was investigated by the transwell, and the apoptosis and cell cycle were assayed by the flow cytometry. (biomedcentral.com)
  • AP-1 transcription factor has been shown to have a hand in a wide range of cellular processes, including cell growth, differentiation, and apoptosis. (wikipedia.org)
  • Adenine induced significant apoptosis in HT29 cells, whereas Caco-2 cells exhibited less apoptotic responses. (hindawi.com)
  • Inhibition of cell viability was assessed with focus on apoptosis transcriptionally and translationally. (hindawi.com)
  • ATZ also induces morphological changes similar to apoptosis and initiates mitochondria-dependent cell death in several experimental models. (frontiersin.org)
  • In order to explore the underlying mechanisms, we performed tests to assess cell migration and motility, apoptosis, expression of glucose transporter 1 and proapoptotic proteins. (oncotarget.com)
  • When in vitro experimental models are exposed to ATZ, they are faced with increased levels of reactive oxygen species (ROS), cytotoxicity and decreased growth rate at dosages that may vary with cell types. (frontiersin.org)
  • However, it is not known if CXCR1 is expressed on the apical or basolateral poles in polarized Caco-2 BBE cells or human intestinal tissue or whether the direction of TLR signalling influences the direction of IL-8 secretion. (biomedcentral.com)
  • Caco-2 cells with a high expression of both HER2 and FASN were selected for functional characterization. (biomedcentral.com)
  • The characterization of BBB cells would help light on the exact mechanism of nanoparticle delivery into the brain, which would enable the design of targeted nanoparticles to deliver drugs to the CNS. (helsinki.fi)
  • In spite of the important source of proteins, polysaccharides, minerals and lipids that can be isolated from food waste, a little attention has been focused on its valorization in the form of functional materials. (mappingignorance.org)
  • Glycosphingolipids (GSLs) are a subclass of glycolipids that are found in cell membranes of various organisms ranging from bacteria to humans. (springer.com)
  • Supports healthy cell membranes. (nutrimal.co)
  • These chemicals' main job is to keep cell membranes intact [26]. (pdfplayer.org)
  • And the influence of FASN on proliferation and migration of colorectal cancer cells was also explored. (biomedcentral.com)
  • The AP-1 transcription factor has been shown to play numerous roles in cell growth and proliferation. (wikipedia.org)
  • C-jun has been shown to be essential for fibroblast proliferation, and levels of both AP-1 subunits have been shown to be expressed above basal levels during cell division. (wikipedia.org)
  • However, the mechanism by which adenine inhibits the proliferation of cancer cells is sketchy. (hindawi.com)
  • In this study, we investigated the effects of adenine against the proliferation of colon cancer cells. (hindawi.com)
  • Other phenolic constituents such as phytic acid was shown to exhibit anticancer properties through the regulation of vital cellular functions such as signal transduction, cell proliferation and differentiation ( Vucenik and Shamsuddin, 2006 ). (scialert.net)
  • Histopathological observations revealed hypertrophy and proliferation in gill epithelium, curling and fusion of secondary gill lamellae (SGL), distortion in chloride and pillar cells under laboratory experiment. (omicsonline.org)
  • Caveolin-2 expression is necessary for the control of E2-dependent cellular proliferation of MCF-7 cells. (cusabio.com)
  • Phospholipids are metabolic intermediates that have a role in the functioning and proliferation of plant cells [9]. (pdfplayer.org)
  • Interestingly, a meta-analysis revealed that supplementation of anthocyanins reduced serum lipid profiles in dyslipidemia patients [ 14 ]. (biomedcentral.com)
  • In 2013, the American Heart Association reported that proportions of American adults aged 20 or above had abnormal blood lipid serum profile (details are shown in Table 1 ) [ 4 ]. (hindawi.com)
  • A downregulation of FASN effectively inhibits the activity of "HER2-PI3K/Akt axis" and alters the malignant phenotype in colorectal cancer cells. (biomedcentral.com)
  • Hence, in this study, we analyzed the potential link between FASN and the activity of "HER2-PI3K/Akt axis" in colorectal cancer cells. (biomedcentral.com)
  • It is of interest to explore the inhibitory effect of adenine on the growth of colorectal cancer cells and to determine the underlying mechanism. (hindawi.com)
  • We explored the possible mechanisms underlying the anticancer activity of adenine in colorectal cancer cells. (hindawi.com)
  • The NBD cholesterol uptake in Caco-2 cells was determined using fluorometric assay. (biomedcentral.com)
  • Cell viability was measured using the MTT assay. (hindawi.com)
  • We used a cell-based human FXR β-lactamase ( Bla ) reporter gene assay to profile the Tox21 10K compound collection of environmental chemicals and drugs. (nature.com)
  • This results in differing cytotoxic responses that are influenced by the nature of target cells, assay types and concentrations of ATZ. (frontiersin.org)
  • In the Caco-2 BBE model of polarized villus-like epithelium, apical stimulation with TLR2 and TLR5 ligands resulted in the apical secretion of IL-8. (biomedcentral.com)
  • Here we investigated IL-8 secretion in response to TLR signalling using intestinal Caco-2 BBE cells grown in the Transwell system. (biomedcentral.com)
  • Stimulation of polarized Caco-2 BBE cells with Pam2CSK4 and Pam3CSK4, agonists for TLR2/6 and TLR2/1 from the apical or basolateral sides induced secretion of IL-8 predominantly into the apical compartment regardless of the location of the stimulus, although basolateral stimulation induced smaller amounts of IL-8 compared to apical stimulation. (biomedcentral.com)
  • Clearance of small intestinal crypts involves goblet cell mucus secretion by intracellular granule rupture and enterocyte ion transport. (gu.se)
  • Vasoactive intestinal peptides (VIP) expression and secretion by EN were assessed by RT-PCR and ELISA, respectively. (emory.edu)
  • For the analysis of GSLs, they are extracted from tissues and cells using organic solvents. (springer.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Also, a decreasing trend of cav-2 (transcripts I and II) were observed in tumoral tissues especially in stages I and II and seem to be associated with the incidence and promotion of breast cancer, especially in the initial stages of breast cancer. (cusabio.com)
  • It is found in all body tissues and plays a role in making red blood cells and maintaining nerve cells and the immune system. (supplemented.net)
  • Two experiments were performed on regular HEK-cells: inhibitor experiment with KYP-2047 (1 or 10 µM) and overexpression experiment (transfection with either active or inactive hPREP plasmid). (helsinki.fi)
  • In neutrophils, IL-8 binding to CXCR1 triggers G protein-coupled signalling and formation of second messengers that mediate cellular migration, exocytosis of effectors and a respiratory burst to facilitate oxygen-dependent killing of phagocytosed microorganisms [ 2 ]. (biomedcentral.com)
  • Atrazine (ATZ) is an environmental pollutant that interferes with several aspects of mammalian cellular processes including germ cell development, immunological, reproductive and neurological functions. (frontiersin.org)
  • It is essential for cell homeostasis and survival and has been related to various diseases and pathophysiology. (helsinki.fi)
  • The aim of the study was to investigate the lipid-lowering mechanisms of C3R through inhibition of lipid digestion and absorption in vitro. (biomedcentral.com)
  • The present findings suggest that C3R acts as a lipid-lowering agent through inhibition of lipid digestion and absorption. (biomedcentral.com)
  • Hypothesis was to see differences in colocalization of Bcl-2 and Beclin 1 in cells treated with different PREP-modifications and for PREP-inhibition to decrease the colocalization. (helsinki.fi)
  • Infected cells secreted the DAMP protein, heat shock protein 70 (HSP70), into the culture media, as revealed by proteomic and Western analyses. (emory.edu)
  • In contrast, globosides are neutral lipid molecules. (springer.com)
  • Lactic acid reacts with the calcium carbonate (CaCO 3 ) present in the exoskeleton of crustaceans, leading to the formation of calcium lactate, which precipitates and could be then removed. (mappingignorance.org)
  • Yeasts can be considered as alternative cell factories to lactic acid bacteria for lactic acid production, despite not being natural producers, since they can better tolerate acidic environments. (biomedcentral.com)
  • The most relevant bottleneck in production by LAB is in all likelihood related to the inhibitory effects of the low pH of the medium on cell growth, cell viability and in turn on lactic acid accumulation. (biomedcentral.com)
  • Identification of PARD3 signature on PARD3 deficient H157 cell line, reconstituting the expression of PARD3 gene, with a wt and a mutant form. (nih.gov)
  • This review focuses on the differential responses of cell types to ATZ toxicity, testicular effects of ATZ in both in vitro and in vivo models and chemopreventive strategies, so as to highlight the current state of the art on the toxicological outcomes of ATZ exposure in several experimental model systems. (frontiersin.org)
  • Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on HeLa cells with SR-BI antibody NB400-104AF488 (blue) and a matched isotype control NBP2-24893AF488 (orange). (novusbio.com)
  • Flow Cytometry: SR-BI Antibody [NB400-104] - Analysis of Huh7 and HepG2 cells using SR-B1 antibody NB400-104. (novusbio.com)
  • The data showed that adenine activated AMP-activated protein kinase (AMPK) signaling contributing to autophagic cell death through mTOR in both colon cancer cell lines. (hindawi.com)
  • Adenine is involved in a variety of cell biological processes and has been explored for pharmacological uses. (hindawi.com)
  • Thioglycosides have received considerable attention because they are widely employed as biological inhibitors [ 2 - 6 ], inducers [ 7 - 9 ]. (oncotarget.com)
  • Results: SARS-CoV-2 infection led to increased expression of BiP/GRP78, a marker and key regulator for ER stress in Caco-2 cells. (emory.edu)
  • Adenine improves the survival of rat Purkinje cells and enhances the storage of erythrocyte in whole blood [ 6 - 8 ]. (hindawi.com)
  • Two novel compounds labelled as thioglycoside A and B were designed and evaluated on breast and colon cancer cell lines. (oncotarget.com)
  • These are natural compounds made from triglycerides in which one of the fatty acids is replaced by a phosphoric group or a nitrogenated base: the fat-related part is the remaining lipid group, while the hydrophilic part is the phosphoric group [14] [15]. (pdfplayer.org)
  • Adenine significantly inhibited the viability of colon cancer cells, HT29 and Caco-2 cells, in a dose-dependent manner. (hindawi.com)
  • Patients without information on CD4 cell counts were significantly less often heterosexual (P = 0.007) and more often had an unknown transmission risk (P (atm-signaling.com)
  • This review summarizes the recent experimental and clinical results of nine representative single Chinese herbs and seven classic TCM formulae that could improve lipid profiles so as to help understand and compare their underlying mechanisms. (hindawi.com)
  • In this study we investigated the mechanisms involved in the induction of oxidative stress and impairment of mitochondrial quality control and mitophagy in hepatocytes by the saturated fatty acid palmitate and Western diet feeding in mice and if their harmful effects could be reversed by the neurotrophic factor glial cell derived neurotrophic factor (GDNF). (emory.edu)
  • These lipid disorders may occur in primary (inherited) [ 5 - 7 ] or secondary form [ 8 ]. (hindawi.com)
  • Extraction procedures are optimized to precipitate and remove proteins and nucleic acids while maximizing solubilization of GSLs along with other lipids. (springer.com)
  • Since rate of autophagy in cells was not detected, it is impossible to determine, if there were changes in autophagy that were not reflected as changes in colocalization of these two proteins. (helsinki.fi)
  • To characterize hCMEC/D3 cells for the presence of specific endocytic pathways, proteins involved into each pathway were selected. (helsinki.fi)
  • Supports energy production by transferring energy from carbs fats and proteins into ATP, the cell's primary energy currency. (nutrimal.co)
  • Hyperlipidemia comprises a heterogeneous group of disorders, characterized by high levels in one or more lipids and/or lipoproteins [atherogenic free fatty acids (FA), triglycerides (TG) (hypertriglyceridemia), small dense low-density lipoprotein cholesterol (LDL-C) (hypercholesterolemia), and apolipoprotein (apo) B], and/or low level in antiatherogenic high density lipoprotein cholesterol (HDL-C), in the circulation [ 1 - 3 ]. (hindawi.com)
  • Autophagy is a pathway for cells to degrade intracellular components that are no longer needed or are detrimental for the cells. (helsinki.fi)
  • Human embryonic kidney cells 293 (HEK-293) and hPREP knockout cell line created from them by using CRISPR/Cas9-silencing were used in the experiments. (helsinki.fi)
  • However, there were no significant differences in colocalization of Beclin 1 and Bcl-2 in any of the experiments but it was nearly 100 percent in all treatments. (helsinki.fi)
  • 2019) Anisotropic circular dichroism of light-harvesting complex II in oriented lipid bilayers: Theory meets experiment. (brc.hu)
  • Indeed, large amounts of CaCO 3 must be added during fermentation, to maintain a constant pH of the culture broth (at around 5) and sustain production. (biomedcentral.com)
  • Both Bcl-2 and Beclin 1 also have functions not related to autophagy, which could be one reason behind the results gained in this study. (helsinki.fi)
  • Induction of autophagy in the latter pathway is dependent on the interaction of Bcl-2 and Beclin 1. (helsinki.fi)
  • The aim of this study was to find out how PREP affects the interaction between Bcl-2 and Beclin 1 and how this affects autophagy. (helsinki.fi)
  • Whole cell extracts (30 µg lysate) of HeLa (Lane 1), SH-SY5Y (Lane 2), A549 (Lane 3), A-431 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). (thermofisher.com)
  • Western Blot: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human adrenal cell lysate. (novusbio.com)
  • Today, it is estimated that about 80 % of people in developing countries rely on traditional medicines for their primary health care. (pharmatutor.org)
  • After immunofluorescence staining, cells were analysed with confocal microscope and colocation analysis of Bcl-2 and Beclin 1 was performed. (helsinki.fi)
  • Immunocytochemistry/ Immunofluorescence: SR-BI Antibody [NB400-104] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. (novusbio.com)
  • The role of DNA methylation on sexual determination and development in zebrafish germ cells. (exeter.ac.uk)