Lines cells cultured. Medical search. Frequent questions

Cell cultured meat, also known as cultivated, cultured, or lab-grown meat, is a product of cellular agriculture , that uses tissue samples from living animals to grow sustainable, humane meat in a bioreactor. (moleculardevices.com)
Agitation based: a cell suspension is placed in a rotating bioreactor. (integra-biosciences.com)
Bioreactor flasks are specialized vessels used to culture cells at a large scale, making them a valuable tool for the production of mAbs. (coleparmer.com)
While hybridoma cells can be cultured in standard culture vessels, bioreactor flasks provide several advantages when scaling up the production process. (coleparmer.com)
Bioreactor flasks have a large surface area and volume, allowing for increased cell density and greater antibody production. (coleparmer.com)
Large Scale Mammalian Cells and Cell clusters expansion plant: GE Wave bioreactor systems with single-use up to 25L capacity bags for suspension cells culture or microcarrier-based adherent cells culture. (weizmann.ac.il)
Sartorius Stedim Biotech combined the company's ambr 15 bioreactor system with the Nova BioProfile FLEX2 cell culture analyzer for laboratory experiments. (biopharminternational.com)
Sartorius Stedim Biotech's (SSB) ambr 15 automated micro-bioreactor system combined with a Nova Biomedical (Nova) BioProfile FLEX2 automated cell culture analyzer was shown for the first time at the Cell Line Development & Engineering Conference in San Diego, California, SSB announced in a June 5, 2017 press release. (biopharminternational.com)
There is a significant demand for fully automated cell culture analysis of small-volume culture systems like the Sartorius ambr micro bioreactor system," said Matthew McRae, biotechnology sales product line manager at Nova, in the press release. (biopharminternational.com)

The evaluation of SPRP as a supplement showed that there was no statistical difference in cell viability compared to cultures supplemented in the standard way, with fetal bovine serum (FBS), after 24 h of culture. (springer.com)
Discover how to thaw cells and maintain viability. (atcc.org)
Because of their requirements for viability, primary cell cultures did not become widespread until the 2000s. (wikipedia.org)
For example they can be used when examining substances effect on cell viability, which can further on be potential treatments for brain deficits. (wikipedia.org)
We'll provide you with tips on reproducibility, contamination, viability and automation to help you overcome the major cell culture challenges. (integra-biosciences.com)
The major downside of suspension cultures is that they require daily cell counts and viability determination to follow growth patterns, whereas adherent cultures can easily be inspected under a microscope. (integra-biosciences.com)
There is a demand for DMSO-free cryoprotectants that maintain cell viability and function after thaw. (nature.com)
Culturing suspension cells can be tricky because they need specialized culture vessels and techniques to maintain their viability and growth. (coleparmer.com)
An improved tumor necrosis factor (TNF) bioassay using an isolated subclone from the murine fibroblastoid NCTC-clone-929 cell line and a new fluorescence indicator system for detecting target cell viability was described. (cdc.gov)
Cytotoxicity was assessed using the MTT assay (cell viability) and Griess reagent (NO release). (bvsalud.org)

Effect of fiber diameter on spreading, proliferation, and differentiation of osteoblastic cells on electrospun poly (lactic acid) substrates. (springer.com)
The influence of fiber diameter of electrospun substrates on neural stem cell differentiation and proliferation. (springer.com)
ReNcell NSC Maintenance Medium (SCM005): ReNcell Neural Stem Cell (NSC) Maintenance Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of ReNcell immortalized human neural progenitor cells. (thomassci.com)
Withdrawal of the growth factors from ReNcell NSC Maintenance Medium will result in the spontaneous differentiation of ReNcell immortalized neural progenitor cells. (thomassci.com)
Additionally, RAD18 functions beyond DNA repair and may regulate many other key biological processes, such as chromatin strengthening, cell survival/death, stemness, and differentiation. (nature.com)
In addition, their osteogenic differentiation potential was increased, and genes involved in cell adhesion, FGF-2 signalling, cell cycle, stemness, cell differentiation, and cell proliferation were upregulated, compared to that of the MSCs cultured on uncoated plates. (hindawi.com)
The differentiation of mesenchymal stem cells (MSCs) into multiple cell lineages can be exploited as an attractive strategy for cell-based therapy and regenerative medicine [ 1 ]. (hindawi.com)
The ECM provides structural and biochemical support to the cells and has various other functions including cell adhesion, cell to cell communication, and differentiation [ 17 , 18 ]. (hindawi.com)
Under this agreement, FDA will oversee the collection, growth, and the differentiation of living cells into various cell types, such as proteins and fats. (usda.gov)
To induce differentiation and maturation of the immortalized cell lines, terminating the HPV-E6/E7 expression through a gene induction system has been believed to be essential. (lu.se)
Here, we report that erythroid cell lines established from human bone marrow using simple expression of HPV-E6/E7 are capable of normal erythroid differentiation, without turning gene expression off. (lu.se)
Upon differentiation, other ELLU clones shift from fetal to adult hemoglobin expression, giving rise to more mature cells. (lu.se)
Our findings propose that it is not necessary to employ gene induction systems to establish immortalized erythroid cell lines sustaining differentiation potential and describe novel cellular characteristics for desired functionally competent clones. (lu.se)

Autologous preparations rich in growth factors promote proliferation and induce VEGF and HGF production by human tendon cells in culture. (springer.com)
When used in conjunction with FGF and EGF, the maintenance medium will allow for the proliferation of ReNcell immortalized VM and CX neural stem cells. (thomassci.com)
High expression of RAD18 facilitated a highly stem-cell phenotype through the Hippo/YAP pathway, which supports the proliferation of TNBC. (nature.com)
Because proliferation of PAW cells is affected by culture conditions, such as a room temperature, aeration conditions of the culture and so on, an amount of cells transferred to fresh medium and the subculture intervals may vary from one lab to another. (riken.jp)
Proliferation of Leukemic Blood Cells in Short-Term Liquid Cultures. (karger.com)
Cell proliferation includes a series of events that is tightly regulated by several checkpoints and layers of control mechanisms. (frontiersin.org)
In conclusion, we provide useful experimental approaches and bioinformatics to identify informative and predictive genes at the single-cell level, which opens up new means to describe and understand cell proliferation and subpopulation dynamics. (frontiersin.org)
However, most of our knowledge about cell proliferation comes from studies that average data from large and mixed cell populations. (frontiersin.org)
1994. Mesothelial cell proliferation: A nonspecific response to lung injury associated with fibrosis. (cdc.gov)

For example, RAD18 plays a vital role in regulating the DNA stability of embryonic stem cells and cellular homeostasis in highly prolific cells [ 6 ]. (nature.com)
The use of human embryonic stem cells (hESCs) in research is increasing and hESCs hold the promise for many biological, clinical and toxicological studies. (nih.gov)
Unlike embryonic stem cells, adult stem cells (MSCs) have a limited lifespan and stop proliferating during in vitro culture due to replicative senescence [ 8 ]. (hindawi.com)

Traditionally, research on the replication of shrimp viruses has been based on the use of cultured fish cellular lines ( 6 ). (cdc.gov)
The cytopathic effect in RD cells began with a partial destruction of the cellular layer. (cdc.gov)
Next, small cellular groups and some isolated round cells were observed. (cdc.gov)
In Hep-2C cells, the cellular monolayer was partially destroyed. (cdc.gov)
The cytopathic effect in the BGM cell line began as a partial destruction of the cellular layer, which evolved to a syncytial-like formation of rounded, refringent cells. (cdc.gov)
Autologous human serum for cell culture avoids the implantation of cardioverter-defibrillators in cellular ardiomyoplasty. (springer.com)
These cultures present several advantages over cell lines, including a better representation of the cellular heterogeneity of tissues, a more faithful transcriptomic and proteomic profile (especially when cultured in 3D) and more realistic functional responses, including drug responses. (wikipedia.org)
Here, we highlight various applications and research methods using our CloneSelect Imager and Single-Cell Printer, ImageXpress Cellular Imaging System, and SpectraMax® microplate reader to help scale your cell line development workflow. (moleculardevices.com)
Seahorse Metabolic Analyzers service for rapid and quantitative measurement of cellular bioenergetics: Seahorse XF Analyzers measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate to interrogate key cellular functions such as mitochondrial respiration and glycolysis. (weizmann.ac.il)
Cellular senescence, which is morphologically characterized by an enlarged and flattened cell shape, was first described by Hayflick [ 9 ]. (hindawi.com)
Cellular senescence refers to active cells that eventually enter a state of irreversible growth arrest. (hindawi.com)
Currently, company executives use a range of terms to describe the field, including but not limited to cultured meat, cultivated meat, cell-based meat, clean meat, cellular agriculture, and lab-grown meat, as well as slaughter-free meat and ethically grown meat, to a lesser extent. (globenewswire.com)
To make food, a small number of cells are taken from the cell bank and placed in a tightly controlled and monitored environment (e.g., a very large, sealed vessel) that supports growth and cellular multiplication by supplying appropriate nutrients and other factors. (usda.gov)
Once the cells have differentiated into the desired type, the cellular material can be harvested from the controlled environment and prepared using conventional food processing and packaging methods. (usda.gov)
In passive cellular immunotherapy, specific effector cells are directly infused and are not induced within the patient. (msdmanuals.com)
Passive cellular immunotherapy references A number of immunologic interventions, both passive and active, can be directed against tumor cells. (msdmanuals.com)
In passive cellular immunotherapy, specific effector cells. (msdmanuals.com)

On March 7, 2019, FDA and FSIS agreed to establish a joint regulatory framework for human foods made from cultured cells of livestock and poultry to help ensure that any such products brought to market are safe, unadulterated, and truthfully labeled. (usda.gov)

HeLa cells) or by genetic modification (e.g. (wikipedia.org)
Back in the 1950's, cells from Henrietta Lacks (now called HeLa cells) were isolated and cultivated in horse serum," recalls Adam Elhofy, Ph.D., CSO, Essential Pharma. (genengnews.com)
Confocal immunofluorescent analysis of HeLa cells, insulin-treated (left) and LY294002-treated (#9901, right), using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP ® Rabbit mAb (Alexa Fluor 488 ® Conjugate) (green). (cellsignal.com)

A total of six siRNAs were designed and screened through transient transfection assay and knockdown in expression of CP-PVY mRNA was calculated in CHO-k cells. (virosin.org)
We developed a calcium signaling-based assay, using cultured human embryonic kidney cells (HEK), that evaluates simultaneously, the activation/desensitization or blockade of the proteinase-activated receptors, PAR 1 and PAR 2 . (aspetjournals.org)
The abilities of the PAR 1 -targeted agents to desensitize or block PAR 1 in the HEK cell assay were compared with their activities in a human platelet aggregation assay. (aspetjournals.org)
Our data illustrate the usefulness of the HEK cell assay for evaluating the PAR 1 /PAR 2 selectivity of PAR-activating agonists. (aspetjournals.org)
To this end, we developed a calcium-signaling assay, employing cultured human embryonic kidney cells (HEK293) in which the action of PAR 1 and PAR 2 agonists and antagonists could be evaluated simultaneously. (aspetjournals.org)

Although the LAT family plays important roles in the development and function of normal tissues, they are frequently increased in cancer cells ( 3 ). (spandidos-publications.com)
In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). (wikipedia.org)
When whole or partial tissues are isolated and maintained ex vivo, the procedure is termed primary tissue culture. (wikipedia.org)
The entire process of going from animal tissues to cultured meat products described above is time-consuming, labor-intensive, and prone to error if done in a manual fashion. (moleculardevices.com)
However, MSCs need to be expanded in vitro in order to obtain sufficient cells for clinical trials since they are extremely rare in various tissues. (hindawi.com)

There are two reasons for culturing naturally adherent cells in suspension. (integra-biosciences.com)
In 2D applications, adherent cells are grown in a monolayer system on a flat surface, e.g. in a T-flask. (integra-biosciences.com)
Scaffold-based 3D methods usually involve growing adherent cells in hydrogel scaffolds. (integra-biosciences.com)
Adherent cells such as fibroblasts, epithelial cells, and endothelial cells need a proper surface to attach to, and improper attachment can lead to cell death. (coleparmer.com)
Adherent cells can quickly reach confluency, meaning the entire surface of the culture vessel is covered with cells, which can lead to nutrient depletion and cell death. (coleparmer.com)
Lastly, detaching adherent cells from the surface for passaging or analysis can be tough and can lead to cell damage. (coleparmer.com)
For adherent cells, use tissue culture flasks or tissue culture dishes with the right coating to promote cell attachment and growth. (coleparmer.com)

Animal cells are usually engineered to produce different biologics, including growth factors, hormones, and antibodies that are commonly used as therapies today. (moleculardevices.com)
In an early study, they fused mouse myeloma and spleen cells from an immunized donor to produce cell lines that secreted anti-sheep red blood cell antibodies. (labmanager.com)
They] can work by recruiting immune elements, delivering toxins, producing CAR-T cells, or as bispecific antibodies that recruit T or NK cells to kill the cancer cells. (labmanager.com)
Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. (cellsignal.com)
While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.com)
Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.com)
T-cell engagers are bispecific antibodies that recruit cytotoxic T cells to kill tumor cells. (msdmanuals.com)

Toll-like receptors (TLRs) enable innate immune cells, such as macrophages, to recognize a wide variety of microbial ligands, thereby promoting inflammation. (frontiersin.org)
Six parasites ont montré une résistance in vitro au stibogluconate de sodium en utilisant le test de détection des amastigotes dans les macrophages J774 murins. (who.int)
SIV antigen has been demonstrated by immunohistochemical methods in lymph node sinus histiocytes, macrophages, and giant cells (14) as well as in macrophage-derived cells in brain tissue from diseased monkeys (8). (cdc.gov)
1998. Activation of alveolar macrophages and peripheral red blood cells in rats exposed to fibers/particles. (cdc.gov)

You may already know that to produce mAbs, large quantities of hybridoma cells are needed. (coleparmer.com)
The hybridoma technique is still used to develop cell lines for mAb production. (labmanager.com)

Cell line development workflows consist of a multistep process involving single-cell cloning, formation and isolation of these discrete colonies, and the phenotypic characterization of these colonies. (moleculardevices.com)
Isolation is possible in a limited number of cell lines and requires trypsin supplementation. (medscape.com)

Molecular Cell Biology. (wikipedia.org)
It is an in vitro tool that aids the understanding of cell biology and the mechanisms of diseases. (integra-biosciences.com)
There is a long history of scientific advances in biology, biochemistry, and engineering that have led to the innovations enabling the growth of animal cells outside of the animal itself, in a controlled environment, for food. (usda.gov)
They are usually indexed NIM with no subheading because the CELL LINE and CULTURED CELLS are just the source of cells to study some aspect of cell biology. (nih.gov)

Some cells remained isolated but with altered morphology ( Figure 1F ). (cdc.gov)
The morphological analysis, carried out on the third and fifth days, did not verify changes in the typical morphology of Vero cells cultured with SPRP. (springer.com)
Primary cells exhibited the morphological characteristics of epithelial and fibroblast cells, but their immortalization and passaging resulted in a predominantly epithelial cell morphology. (bvsalud.org)
cell authentication (STR), pluripotency and undifferentiated state confirmation, molecular karyotype, g-banding and iPS growth pattern and morphology analysis. (lu.se)

Cultured meat (CM) is an emerging technology, an alternative to the traditional farming industry, and crucial to supply the increasing meat demand while being more environmentally and animal friendly. (moleculardevices.com)
Because cell-cultured meat is made with animal cells, many processes for developing and scaling a cell-cultured meat product will mirror those in biologics production, specifically cell line development . (moleculardevices.com)
Is cell-cultured meat approved? (moleculardevices.com)
In July 2019, several states "declared war" on advertising plant- or cell-based products as "meat. (provisioneronline.com)
It describes the current state of cultured meat research, as well as emerging technologies, patents, funding events, scientific papers, strategic partnerships, and the market's presumed trajectory toward global adoption. (globenewswire.com)
The number of startups focused on developing cultured meat (and the required cell culture media, supplements, and methods to produce them) has been rising year after year. (globenewswire.com)
There are now an impressive 99 companies worldwide developing cultured meat components, services, and end-products, compared to only four in 2016. (globenewswire.com)
Additionally, nearly 40 life science firms have declared and formally launched products to supply market competitors with the essential inputs they need to support cultured meat and seafood production. (globenewswire.com)
Because cultured meat is an early-stage industry, the language to describe it is evolving in real-time. (globenewswire.com)
The potential of cultured meat has captured the imagination of investors, researchers, and consumers alike. (globenewswire.com)
Although the cultured meat market is a nascent industry, 2020 was a landmark year because a cultured chicken product developed by the company Eat Just made its debut on a restaurant menu in Singapore, after the country's food agency approved its sale to the public. (globenewswire.com)
Flow of capital into the cultured meat industry has also grown substantially in recent years, reaching approximately $1.0 billion per year. (globenewswire.com)
Major investments within the cultured meat market have included Cargill's investment into Memphis Meats and Aleph Farms, as well as Tyson Foods Venture Fund's investments into Memphis Meats and Future Meat Technologies. (globenewswire.com)
Wealthy investors are also piling into the cultured meat market. (globenewswire.com)
Another notable name in cultured meat investing is Dr. Rick Klausner, who is a lead investor in the Dutch company Meatable. (globenewswire.com)
Additionally, the cultured meat industry has witnessed public-sector R&D funding awards within the U.S. and the European Union, and China signed a $300 million deal to buy lab-grown meat produced in Israel in a deal that openly signals its intent to supply cultured meat to its massive population of 1.4 billion citizens. (globenewswire.com)
By 2040, a projected 60% of the meat will be created from cells grown within bioreactors and sold across grocery stores and restaurants worldwide. (globenewswire.com)
The rise of the cultured meat market will be supported by the sustainability of the process, as well as the industry's ability to provide "tailor-made nutrition" through its meat and seafood products. (globenewswire.com)
Recent surveys indicate that nearly 50% of consumers do not have reservations about consuming cultured meat. (globenewswire.com)
Over the next 10 to 20 years, the cultured meat market is projected to act as a major disruptor to the conventional meat market. (globenewswire.com)

RD, Hep-2C, and BGM cells injected with an extract similarly processed but from healthy shrimp, showed no cytopathic effects, even after 7 days of culture ( Figures 1A , 1C , and 1 E ). As a positive control, RD cells were injected with Sabin viral extract and showed the characteristic cytopathic effect produced by an enterovirus infection. (cdc.gov)
They are readily detected when antibiotics are not present, but when antibiotics are used routinely in cell culture protocols, it can cause a slow-growing infection that is difficult to detect. (corning.com)
Here, we have used cocultures of neuroglioma (H4) and glioblastoma (U118-MG) cells as a minimal in vitro model to investigate whether Aβ is produced by neuroglioma cells and whether this could result in protective anti-viral activity against HSV-1 infection. (iospress.com)
Production of pro-inflammatory cytokines TNFα and IL-1β by H4 and U118-MG cells occurred under basal conditions but infection of the cells with HSV-1 did not significantly upregulate production. (iospress.com)
Our data established that H4 neuroglioma cells produced Aβ42 in response to HSV-1 infection thus inhibiting secondary replication. (iospress.com)
Because of the risk of infection with SARS-CoV-2 among laboratory workers, CDC recommends that laboratories discontinue the use of the A549/MV1LU mix when they perform respiratory viral culture panels. (cdc.gov)
Immunosuppressed persons, such as stem cell transplant or organ transplant recipients, may require CT scanning to clarify upper respiratory versus lower respiratory infection. (medscape.com)
T whipplei infection is recognized to be a major cause of culture-negative endocarditis. (medscape.com)

Organelle Transport in Cultured Drosophila Cells: S2 Cell Line and Primary Neurons. (jove.com)
ReNcell CX Immortalized Cells (SCC007): ReNcell CX is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. (thomassci.com)

Discover best practices how to subculture adherent and suspension cultures. (atcc.org)
There are two basic systems for growing cells: adherent and suspension cultures. (integra-biosciences.com)
While only a few cell types naturally grow in suspension (e.g. lymphocytes), many adherent cell types can be adapted to suspension cultures. (integra-biosciences.com)
The first advantage of suspension cultures is that it's easier to passage the cells, as you don't need to detach them from a culture vessel by enzymatic or mechanical dissociation. (integra-biosciences.com)
Secondly, suspension cultures are easier to scale up, as the cell growth is only limited by their concentration in the medium, not by the available surface area. (integra-biosciences.com)
Sakuta M, Takagi T, Komamine A (1986) Growth related accumulation of betacyanin in suspension cultures of Phytolacca americana L. Journal of Plant Physiology 125: 337-343. (riken.jp)
Characterization of carrot cell lines resistant to 5MT obtained by irradiating suspension cultures with UV-light. (unipv.it)

FSIS and FDA have held public meetings to better understand the science of animal cell culture technology, potential hazards, labeling considerations, and to listen to consumer concerns. (usda.gov)
Regulatory jurisdiction transitions from FDA to FSIS during the harvesting stage of the cell-culturing process. (usda.gov)

In addition, the presence of EVs reduced inflammatory responses in Pam 3 CSK 4 -treated endothelial cells and HEK Dual reporter cells, demonstrating that TLR2-EVs can act as decoy receptors. (frontiersin.org)
The neurovascular unit (NVU) contains endothelial cells joined by tight junctions that shield the brain from the blood via the blood brain barrier (BBB). (lu.se)
They are required to support the endothelial cells and to maintain the BBB together with endothelial cells and astrocytes. (lu.se)
Human brain vascular pericytes treated with either scrambled or RGS5 targeting siRNA, will be co-cultured in a spheroid model of the BBB together with human brain endothelial cells and human astrocytes. (lu.se)

This essential laboratory process covers the thaw, growth, propagation, and cryopreservation of cells that have been isolated from a donor organism. (atcc.org)
Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines. (wikipedia.org)
Neuronal primary cell cultures are cells collected from the brain of an organism. (wikipedia.org)
The organism has been cultured from affected cerebrospinal fluid (CSF) and vitreous humor of patients with Whipple disease. (medscape.com)

These studies demonstrated that a combination of three different osmolytes including sugar, sugar alcohol and amino acids/proteins could stabilize Jurkat cells and mesenchymal stromal cells (MSCs) during freezing. (nature.com)
Monoclonals can be used directly to bind to surface proteins on cancer cells to kill or inhibit their growth," explains Dr. Moran. (labmanager.com)
Poly-L-lysine (PLL) of extracellular matrix proteins is a small natural homopolymer of the essential amino acid L-lysine that is used to coat culture substrates. (hindawi.com)
Few balancing acts are as delicate as the optimization of a cell expression system, especially if the expression system is to yield recombinant proteins destined for therapeutic use. (genengnews.com)
Further, there were no significant changes in metabolites or increases in biomass such as cell debris and host proteins. (genengnews.com)
This is unsatisfying since strong spectra are often produced by high abundant proteins, which typically represent the bulk of the cell activity. (lu.se)
Chimeric antigen receptors (CAR) recognize specific proteins on the surface of tumor cells. (msdmanuals.com)
In contrast to TCR T cells, CAR T cells recognize only relatively large proteins on the surface of tumor cells. (msdmanuals.com)

Since Sabin strain LSc 2ab (Sabin 1), the poliovirus used for human vaccination, is usually replicated in monolayer culture cells of human rhabdomyosarcoma (RD), human larynx carcinoma (Hep-2C) (), or Buffalo green monkey kidney (BGM) ( 7 ), we injected these cell lines with a 0.22-µm membrane-filtered whole extract of the hepatopancreas of shrimp ( Penaeus stylirostris ) affected with TSV. (cdc.gov)
Immortalized by retroviral transduction with the c-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. (thomassci.com)
Cell contamination is a major concern in 3D cell culture labs as well as with more traditional 2D monolayer cultures. (corning.com)

Strains of SIV have been successfully cultured in human lymphocyte cell lines (e.g. (cdc.gov)
SIV appears to be primarily tropic for CD4 (T4)-positive leukocytes and has not been successfully propagated in B-lymphocyte cell lines (e.g. (cdc.gov)
Although antilymphocyte serum was used in the treatment of chronic lymphocytic leukemia and in T-cell and B-cell lymphomas, resulting in temporary decreases in lymphocyte counts or lymph node size, newer humoral immunotherapeutic modalities have been developed. (msdmanuals.com)

abstract = "Glial cell line-derived neurotrophic factor (GDNF) has been shown to counteract seizures when overexpressed or delivered into the brain in various animal models of epileptogenesis or chronic epilepsy. (lu.se)

A culture medium provides the cells with most of the nutrients such as amino acids, carbohydrates, vitamins, and minerals they need for metabolism. (atcc.org)
The company is currently evaluating the serum-free supplement on other aspects of bioprocessing, including single-use bioreactors, protein quality, and cell metabolism. (genengnews.com)

These comprise basic cell culture technology, e.g. cell cultivation and expanding cell cultures, but also authentication and contamination tests. (dsmz.de)
Because of the invaluable nature and numerous applications of cell lines in life science research, it is critical that they are handled carefully to not only avoid contamination but to protect human safety. (atcc.org)
Say goodbye to cell culture contamination nightmares and hello to happy cells. (coleparmer.com)
How do you devise cell culture protocols that prevent contamination ? (corning.com)
What Are the Causes of Cell Contamination? (corning.com)
The major causes of contamination in the cell culture system are typically either chemical or biological. (corning.com)
In 3D cell culture, contamination can occur in various components, such as the bio-ink or the extracellular matrix . (corning.com)
Chemical contamination is the presence of a nonliving substance that may cause unwanted effects on the cell culture. (corning.com)
When you've discovered contamination, autoclaving is recommended to cure the contaminated culture and prevent it from spreading to other cultures. (corning.com)
Understanding cell culture contamination best practices ensures that your research quality does not have to suffer due to an unexpected occurrence, whether big or small. (corning.com)

Advancements in cell culture technology enable scientists to use animal cells obtained from livestock, poultry, or seafood to produce food products. (usda.gov)
Regulatory oversight of human foods comprised of or containing cultured animal cells depends on the animal species used as the original source of cultured cells, and it is based on the agencies' existing jurisdiction over products. (usda.gov)

Be a part of the movement to raise credibility in science and order your cell line authentication and mycoplasma testing services today. (atcc.org)
In many cases, cell lines have been misidentified, contaminated with other cells or infected with Mycoplasma, small intracellular bacteria that went undetected for decades. (wikipedia.org)
This is usually only tried with mycoplasma infections, as fungus and yeast contaminants are unlikely to be eradicated, bacterial contaminants are often already antibiotic-resistant, and many cultures lose critical characteristics after cleanup. (corning.com)
Deliver 2 clonally expanded mycoplasma free iPSC lines (15 x 300k cells). (lu.se)

FBS derived from clotted blood is the most widely used undefined supplement in eukaryotic, especially mammalian, cell culture. (sigmaaldrich.com)
Mammalian cell lines are most commonly used for recombinant antibody production, although cell lines of bacterial, yeast, or insect origin are also suitable. (cellsignal.com)

Tositumomab is used in the treatment of some B-cell non-Hodgkin lymphomas and is an example of a monoclonal antibody used to deliver toxins, as some of the antibody delivers a linked radioactive substance to the B-cells. (labmanager.com)

Best practices for reviving cells from cryopreservation are critical to ensuring unambiguous experimental results in basic research and industrial processes. (atcc.org)
These best practices form the basis of several cell culture guides , webinars , and video tutorials that detail the initiation, expansion, and cryopreservation of continuous cell lines, primary cells, hTERT-immortalized primary cells, and organoids. (atcc.org)
Dimethyl sulfoxide (DMSO) has been the standard cryopreservation agent for freezing cells since the 1960 s 2 . (nature.com)

Due to their simplicity, 2D techniques can't mimic the cells' in vivo environment, where they usually grow in three dimensional structures with complex cell-to-cell interactions. (integra-biosciences.com)
Ex vivo manufactured red blood cells (RBC) generated from immortalized erythroid cell lines which can continuously grow are expected to become a significant alternative in future transfusion therapies. (lu.se)

Multiple methods of isolating cells for culture exist. (atcc.org)
Here, we prompted to compare the chromosomal stability of three hESC lines maintained in the same laboratory using identical culture conditions and passaging methods. (nih.gov)
In feeders, the HS181, SHEF-1 and SHEF-3 hESC lines were chromosomally stable up to 185 passages using either mechanical or enzymatic dissection methods. (nih.gov)
These results support the concept that feeder-free conditions may partially contribute to hESC chromosomal changes but also confirm the hypothesis that regardless of the culture conditions, culture duration or splitting methods, some hESC lines are inherently more prone than others to karyotypic instability. (nih.gov)

FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems. (sigmaaldrich.com)
Feeder cells can provide essential growth factors, nutrients, and signaling molecules to support the growth of cells that are difficult to culture on their own. (coleparmer.com)
Every expression system-every scheme for optimizing nutrients and cell culture conditions-presents advantages, disadvantages, and tradeoffs with respect to costs, yields, quality metrics, contaminants, availability of system components, time constraints, and so on. (genengnews.com)
After the cells have multiplied many times over into billions or trillions of cells, additional factors (e.g., protein growth factors, new surfaces for cell attachment, additional nutrients) are added to the controlled environment to enable the cells to differentiate into various cell types and assume characteristics of muscle, fat, or connective tissue cells. (usda.gov)

CellCelector Robotic System service: The ALS CellCelector is a freely configurable tool for the automated picking of single cells, cell colonies and cell clusters utilizing morphological and fluorescence features of a target. (weizmann.ac.il)
The molecular processes behind cell cycle progression have been dissected by numerous morphological studies on live or fixed single cells using a plethora of techniques to visualize components and processes during cell division. (frontiersin.org)

At this point, the creator of the cell line must perform some form of authentication and characterization that identifies it as a unique cell line. (atcc.org)
basic characterization, including cell authentication (STR), undifferentiated state (FACS) and molecular karyotyping. (lu.se)

Nomad Foods and BlueNalu have agreed to explore the introduction of cell-cultured seafood in Europe, where demand for sustainably produced, healthy seafood products continues to grow. (thefishsite.com)
The new agreement includes a collaboration to conduct market research and identify consumer insights, assess regulatory requirements, and explore new business and product opportunities that would be unlocked by the introduction of cell-cultured seafood throughout Europe. (thefishsite.com)
The agreement represents the first of its kind in Europe between a consumer packaged goods company and a cell-cultured seafood company, and underpins Nomad Foods' commitment to sustainable growth through the development and scaling of emerging food technologies. (thefishsite.com)
The agreement also signifies a mutual interest in the commercialisation of cell-cultured seafood in Europe to help meet rising demand and support wider efforts to safeguard the long-term availability of quality, affordable seafood that is healthy for people and sustainable for the planet. (thefishsite.com)
Their breakthrough technology in cell-cultured seafood perfectly aligns with our purpose and will enable us to continue to introduce innovative and great tasting seafood products that are good for people, good for the planet and accessible to all. (thefishsite.com)

While the authentication of human cell lines has been addressed with STR profiling, up until now the validation of mouse cell lines has been limited at the species level. (atcc.org)
The Plant Cell Culture Library (PCCL) at UMass Amherst is a globally unique collection of callus cell cultures representing more than 1,000 diverse plant species. (umass.edu)

However, T whipplei bacteria have been grown successfully in HEL (a human fibroblast line) cells. (medscape.com)

Brunner D, Frank J, Appl H, Schöffl H, Pfaller W, Gstraunthaler G. Serum-free cell culture: the serum-free media interactive online database. (springer.com)
The transport of serum amino acids into cells is an active process that is facilitated by plasma membrane-localized amino acid transporters. (spandidos-publications.com)
ReNcell NSC Freezing Medium (SCM007): ReNcell NSC Freezing Medium is qualified for use with ReNcell immortalized human neural progenitor cell lines, CX (CHEMICON Catalog No. SCC007) and VM (CHEMICON Catalog No. SCC008) cultured in serum-free conditions with ReNcell NSC Maintenance Medium (CHEMICON Catalog No. SCM005). (thomassci.com)
Qualified for use in cell culture applications, especially serum-free cell culture. (sigmaaldrich.com)
Additional growth factors and hormones are added to promote growth or attachment to the culture vessel, often via undefined adjuncts such as serum. (atcc.org)
Once there is sufficient antibody titer in the serum, the spleen is removed to create hybridomas by fusing spleen cells with a cell line. (labmanager.com)
For example, with animal cell cultures, the trend is away from serum and toward defined media, that is, media uncomplicated by the presence of animal-origin components. (genengnews.com)
Use of serum to culture cells dates back nearly 70 years and continues to this day, albeit with less devotion. (genengnews.com)
We developed Cell-Ess ® , a novel lipid supplement produced under GMP conditions that serves as a serum-replacement, Dr. Elhofy explains. (genengnews.com)
To facilitate cell culture optimization, Essential Pharma has developed Cell-Ess, a serum replacement that is free of animal-origin components. (genengnews.com)
Incubation of the cells in different sera and varying serum concentrations did not indicate any significant differences between sera, but revealed a significant reduction in sensitivity if less than 15% serum was used. (cdc.gov)

The cells are generally grown in plastic or glass vessels. (atcc.org)
Adherent cultures are grown on an artificial substrate, whereas cells grown in suspension are free-floating in the culture medium. (integra-biosciences.com)
This is why some experiments are conducted using 3D cultures, which can be grown using scaffold-based or scaffold-free techniques. (integra-biosciences.com)
The PAW cells are grown on a Murashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), and solidified with 1.2% (w/v) agar, pH 5.8. (riken.jp)
Some cells from the sample are selected, screened, and grown to make a "bank" of cells to store for later use. (usda.gov)
Lymphokine-activated killer (LAK) cells are produced from the patient's T cells which are extracted from the tumor and grown in a cell culture system with the lymphokine interleukin-2 (IL-2). (msdmanuals.com)
These cells are grown in culture in a manner similar to LAK cells. (msdmanuals.com)

Since epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors, including gefitinib (GEF) have been reported to induce the apoptosis of several cancer cell lines, in the present study, we examined whether the cytotoxic effects of GEF are further enhanced under amino acid starvation (AAS) culture conditions. (spandidos-publications.com)
mAbs are used to target and bind tumor cells expressing cancer-specific antigens and induce cell death. (labmanager.com)

More specific terms include organotypic culture, tissue slices and explants. (wikipedia.org)

The right culture vessel is crucial here, and different cell types may require different surfaces, such as plastic, glass, or coated surfaces. (coleparmer.com)
Others use dextran, but it adheres to culture vessel walls and subsequently removes cholesterol from the media creating a sink. (genengnews.com)

Once founded, the cell line can be propagated into several flasks and frozen. (atcc.org)
And for feeder cells, use flasks or dishes with a proper surface and growth media to support their growth and function. (coleparmer.com)

We demonstrated that Cell-Ess increased titers 20-40% in bioreactors. (genengnews.com)
SSB and Nova have collaborated to integrate the software interface that controls the ambr 15 workstation for automated parallel processing of 24 or 48 single-use bioreactors (10-15-mL working volume) with the cell culture analysis capabilities of the BioProfile FLEX2. (biopharminternational.com)

We hold NCI60 human tumor cell library. (weizmann.ac.il)
There are many specific cell lines and tumor cell lines in MeSH. (nih.gov)

In human bronchial epithelial cell lines (16HBE), we used CFTR siRNA knockdown and functional inhibition (CFTR INH -172) to connect loss of CFTR to ENG synthesis. (nih.gov)
For investigation of RGS5, we hypothesize that silencing RGS5 expression using siRNA technology (5) leads to secretome changes that effect cells at the BBB. (lu.se)

The U.S. Food and Drug Administration (FDA) approved two CAR T-cell therapies in 2017, Kymriah developed by Novartis for the treatment of children with acute lymphoblastic leukemia and Yescarta developed by Kite for adults with advanced lymphomas. (nature.com)
Clinical trials of LAK cells in humans are ongoing but this approach has not gained widespread use and is generally considered less effective than other cell therapies. (msdmanuals.com)

Most freshly isolated, primary cell cultures undergo senescence, the process by which cells stop dividing after undergoing a certain number of cell divisions. (atcc.org)

Cells were placed in direct contact with freshly prepared endodontic sealers in polypropylene tubes. (bvsalud.org)

ReNcell CX Human Neural Progenitor Cell Line & Culture Media Kit ReNcell CX Kit contains human neural progenitor cells, optimized maintenance media & freezing media for culture of human neural stem cells. (thomassci.com)
ReNcell CX was developed by the ReNeuron Group plc, a biotech company that specializes in using human somatic stem cells for therapeutics. (thomassci.com)
Mesenchymal stem cells (MSCs) possess great therapeutic potential. (hindawi.com)
Elena has a long-standing interest in the genetic and physiological regulation of mammalian pigmentation, with a special focus on determination of neural crest stem cells. (selectbiosciences.com)
We have established a novel type of pluripotent neural crest-like stem cells (NCLSCs) from neonatal mouse epidermis (Sviderskaya et al. (selectbiosciences.com)

However, primary cells require adequate substrate and nutrient conditions to thrive and after a certain number of divisions they acquire a senescent phenotype, leading to irreversible cell cycle arrest. (wikipedia.org)
The extracellular matrix (ECM) provides structural and biochemical support to the surrounding cells, and it has been used as a coating substrate for cell culture. (hindawi.com)

Also, SIV is distinct from simian T-cell lymphotropic virus type I (STLY-I) which shares extensive genomic sequences with human T-lymphotropic virus type I and is associated with T-cell lymphomas in nonhuman primates (12). (cdc.gov)

We sought to extend the receptor-desensitization paradigm for use with a cultured cell system, in which an intracellular calcium signal rather than a contractile response might be used as an index of receptor activation. (aspetjournals.org)
The four lines were tested for the intracellular content of some free amino acids. (unipv.it)

For the cell culture into the scaffold, it is necessary to establish certain conditions, such as the type of supplementation of the culture medium. (springer.com)
Under AAS culture conditions, the cell killing effect of GEF was synergistically pronounced in the EGFR-expressing cell lines, namely, CAL 27, Detroit 562, A549 and PANC-1 cells compared with those treated with either GEF or AAS alone. (spandidos-publications.com)
The presence of necrostatin-1, an inhibitor of receptor-interacting serine/threonine-protein kinase 1 (RIPK‑1), but not that of Z-VAD-fmk, attenuated the cytotoxic effects of GEF under AAS culture conditions. (spandidos-publications.com)
Electron microscopy demonstrated that the CAL 27 cells treated with GEF under AAS culture conditions exhibited swelling of the cytosol and organelles with an increased number of autophagosomes and autolysosomes, but without chromatin condensation and nuclear fragmentation. (spandidos-publications.com)
These results strongly suggest the induction of necroptosis in response to GEF under AAS culture conditions. (spandidos-publications.com)
Therefore, the enhanced cytotoxic effect of GEF under AAS culture conditions is thought to be mediated by atypical necroptosis. (spandidos-publications.com)
Cells isolated via these techniques are then incubated in carefully maintained conditions, usually at atmosphere plus 5% CO 2 , 37 o C, and 85-95% humidity. (atcc.org)
During the scale-up process, it is important to maintain productivity and genetic stability, and robustness of the cells, which requires optimization of culture conditions. (moleculardevices.com)
Cell culture refers to the process of growing cells under controlled conditions outside their natural environment. (integra-biosciences.com)
They also can control environmental conditions, such as temperature, pH, and oxygen levels, to optimize cell growth and antibody production. (coleparmer.com)
Primary cells have a limited lifespan and demand careful handling and specialized conditions to maintain their original properties. (coleparmer.com)
They are usually much more sensitive to culture conditions. (coleparmer.com)
Molecular cytogenetic analyses performed in three different hESC lines maintained in parallel in identical culture conditions revealed significant differences among them in regard to their chromosomal integrity. (nih.gov)
Despite the three hESC lines were maintained under identical conditions, each hESC line behaved differently upon being transferred to a feeder-free culture system. (nih.gov)
The two younger hESC lines, HS181 (71 passages) and SHEF-3 (51 passages) became chromosomally unstable shortly after being cultured in feeder-free conditions. (nih.gov)
Maintain aseptic conditions of the cell culture, and work in a laminar flow cabinet. (riken.jp)
The combined system provides a tool to simultaneously run, sample, and analyze a massive number of cell culture conditions during cell line and media development by design of experiments for quality-by-design studies. (biopharminternational.com)
Through simply changing cell culture conditions, a newly established cell line, Erythroid Line from Lund University (ELLU), is able to differentiate toward mature cells, including enucleated reticulocytes. (lu.se)
The cell tropism of SIV in culture depends partially on the strain of virus propagated and conditions of cell culture. (cdc.gov)

However, the progenitor cells consist of T cells isolated from resected tumor tissue. (msdmanuals.com)

In this study, we have aimed to improve the functionality and stemness of MSCs during culture using poly-L-lysine (PLL). (hindawi.com)

Target cells were released from attachment, centrifuged, counted, and diluted to a density of 2x10(4) cells per well before a 24 hour incubation with TNF-alpha standards or unknowns. (cdc.gov)

They represent the first line of defense against pathogens in the lower airspace and recognize microbial ligands via pattern recognition receptors ( 4 , 5 ). (frontiersin.org)
Furthermore, we reasoned that, provided receptor cross-desensitization did not occur, it would be advantageous to assess PAR-targeted ligands in a cell that expressed both receptors. (aspetjournals.org)
With both receptors present in the same cell, the selectivity or nonselectivity of a variety of compounds that would affect PAR 1 and/or PAR 2 could be efficiently evaluated in a single experiment. (aspetjournals.org)

Effects of heregulin-beta1 on neural crest cultures and melanoblast cell lines. (fiu.edu)

Recently, several studies have analysed the genomic stability of several hESC lines maintained after prolonged in vitro culture but controversial data has been reported. (nih.gov)

Monoclonality verification is crucial from a quality and regulatory perspective to ensure that the desired characteristics from the colony of cells originate from a single cell. (moleculardevices.com)
Most chemical contaminants are found in the cell culture media and originate from either reagents, the water used to make the reagents, or additives to supplement reagents. (corning.com)

Microscopic image of the subcuticular tissue of the pleopod from a shrimp infected with the supernatant of the third passage of a human larynx carcinoma (Hep-2C) cell culture inoculated with an. (cdc.gov)
To confirm the presence of TSV in the cell culture media, a bioassay was performed by using media from the third passage. (cdc.gov)
Karyotype analyses indicate that the ReNcell CX retains a normal diploid karyotype in culture even after prolonged passage (>45 passages). (thomassci.com)
This method ensures the protection of original stock and the consistent availability of low-passage vials of cells via the creation of a master cell bank and working cell banks. (atcc.org)
This facility allows for walk-in usage by users and will soon provide services including cell passage, storage, transfection, etc. (umass.edu)
The HS181 line gained a chromosome 12 by passage 17 and a marker by passage 21, characterized as a gain of chromosome 20 by SKY. (nih.gov)
Similarly, the SHEF-3 line also acquired a trisomy of chromosome 14 as early as passage 10. (nih.gov)

Anatomy7

Organisms1

Chemicals and Drugs2

Analytical, Diagnostic and Therapeutic Techniques and Equipment4

Phenomena and Processes14

Information Science3

Bioreactor9

Viability10

Differentiation13

Proliferation9

Embryonic stem3

Cellular17

Livestock and poultry1

HeLa3

Assay5

Tissues5

Adherent cells7

Antibodies7

Macrophages4

Hybridoma2

Isolation2

Biology4

Morphology4

Meat20

Infection8

Neurons2

Suspension cultures7

FSIS2

Endothelial cells4

Organism4

Proteins8

Monolayer3

Lymphocyte3

Abstract1

Metabolism2

Contamination10

Animal cells2

Mycoplasma4

Mammalian cell2

Monoclonal1

Cryopreservation3

Vivo2

Methods4

Nutrients4

Morphological2

Characterization2

Seafood5

Species2

Human fibroblast1

Serum11

Grown7

Induce2

Organotypic1

Vessel2

Flasks2

Bioreactors2

Tumor cell2

SiRNA2

Therapies2

Primary cell cultures1

Freshly1

Stem cells5

Substrate2

Lymphomas1

Intracellular2

Conditions19

Progenitor cells1

Stemness1

Attachment1

Receptors3

Neural crest1

Vitro culture1

Originate2

Passage7