• HeLa
  • The origins of some immortal cell lines, for example HeLa human cells, are from naturally occurring cancers. (wikipedia.org)
  • Major examples include human HeLa cells that were obtained from a cervical cancer, mouse Raw 264.7 cells that were subjected to mutagenesis and then selected for cells which are able to undergo division. (wikipedia.org)
  • Contaminated cell lines should never be used for research demanding the specific type of cell line they are assumed to be, and most of them should ideally be discarded or at least not used in research at all, except when the contaminant cells have acquired novel characteristics (e.g., by mutation or viral transfection, for example the HeLa derivate Det98) and thus constitute a novel lineage after all. (wikipedia.org)
  • It is worth noting that the widespread contamination with HeLa cells was initially recognized by Walter Nelson-Rees using simple Giemsa stain karyotyping under a light microscope. (wikipedia.org)
  • This technique works well in recognizing HeLa because these cells have distinctive chromosome aberrations. (wikipedia.org)
  • It is good practice to periodically check cell lines maintained under laboratory conditions (i.e., not placed in long-term storage) for contamination with HeLa or other common contaminants, to ensure that their quality and integrity is maintained. (wikipedia.org)
  • WI-38 was invaluable to early researchers, especially those studying virology and immunology, since it was a readily-available cell line of normal human tissue, unlike the HeLa line, which were cancerous cells. (wikipedia.org)
  • gene
  • 1. Does anybody have a set protocol that describes the procedure starting from trancfecting cells to performing gene expression studies using stably transfected cells? (bio.net)
  • citation needed] Introduction of a viral gene that partially deregulates the cell cycle (e.g., the adenovirus type 5 E1 gene was used to immortalize the HEK 293 cell line). (wikipedia.org)
  • densities
  • I always plate freshly thawed cells at very high densities (unless there is a good reason not to) to get the fastest recovery from the freeze. (bio.net)
  • ATCC
  • 32:857- ATCC designat CCL 16 comments derived from 8 motn old male adult How many different V79 cell lines are there? (bio.net)
  • The B103 and B104 lines have appeared in the literature recently, but I am unable to locate the lines at ATCC, JCTA, etc. (bio.net)
  • ATCC hTERT immortalized chondrocyte fibroblasts have an extended lifespan, and are karyotypically, morphologically, and phenotypically similar to the primary parent cells. (atcc.org)
  • ATCC hTERT immortalized dermal microvascular endothelial cells have an extended lifespan, express a panel of endothelial cell surface proteins, undergo tubule formation in culture and are karyotypically, morphologically, and phenotypically similar to the primary parent cells. (atcc.org)
  • Novel cell lines should be proliferated and distributed and/or deposited at a safekeeping institution such as the ATCC as soon as possible after establishment, to minimize the odds that the line becomes spoiled by contamination. (wikipedia.org)
  • undergo
  • This finding would later be known as the Hayflick limit, which indicated that normal human cells could only undergo a limited number of divisions, and later contributed to the discovery of the biological roles of telomeres. (wikipedia.org)
  • viable
  • Also, if your freeze killed a lot of the cells, the viable cell plating density after thawing is even lower. (bio.net)
  • However, during this period of research, the team also discovered that if cells were properly stored in a freezer, cells would remain viable and could provide enormous numbers of cells for research purposes. (wikipedia.org)
  • growth
  • Cells that are fairly density dependent for growth (NIH 3T3 cells, for ex. (bio.net)
  • The derived cell clones could be classified according to their growth properties in vivo into one (1) non-tumorigenic (growing as surface epithelia) and two tumorigenic groups, forming either (2) benign cysts or (3) well-differentiated squamous cell carcinomas. (springer.com)
  • Working in viruses of aquaculture we have a number of raggedy primary monocultures that we have been tiptoeing around in the hopes of getting a cell line for routine use in screening.Are there any methods involving growth enhancing chemicals or carcinogens that may help speed this process up? (bio.net)
  • Growth and Maintenance of Insect cell lines (PDF). (wikipedia.org)
  • Because of slow growth rates and easy detachment of the original NCI-H295 strains, efforts were made to select a population of cells with better monolayer attachment and more rapid growth. (wikipedia.org)
  • Phenotypic
  • Boukamp P, Tilgen W, Dzarlieva RT, Breitkreutz D, Haag D, Riehl RK, Bohnert A, Fusenig NE (1982) Phenotypic and genotypic characteristics of a cell line from a squamous cell carcinoma of human skin. (springer.com)
  • culture
  • After considering starting cell culture of astrocytes does anyone have any comments as to the usefulness of bought cell lines such as the c6 glioma line? (bio.net)
  • Together, we offer the most diverse selection of cell culture products and services available. (sigmaaldrich.com)
  • Experiments using cell culture are used for developing new diagnostic tests and new treatments for diseases. (wikipedia.org)
  • proteins
  • The cells are used for a wide variety of purposes, from testing toxicity of compounds or drugs to production of eukaryotic proteins. (wikipedia.org)
  • Artificial expression of key proteins required for immortality, for example telomerase which prevents degradation of chromosome ends during DNA replication in eukaryotes Hybridoma technology, specifically used for the generation of immortalized antibody-producing B cell lines, where an antibody-producing B cell is fused with a myeloma (B cell cancer) cell. (wikipedia.org)
  • biology
  • Immortal cell lines are a very important tool for research into the biochemistry and cell biology of multicellular organisms. (wikipedia.org)
  • This simplifies analysis of the biology of cells which may otherwise have a limited lifetime. (wikipedia.org)
  • This can alter the biology of the cell and must be taken into consideration in any analysis. (wikipedia.org)
  • The cell line, isolated by Leonard Hayflick and Paul Moorhead in the 1960s, has been used extensively in scientific research, with applications ranging from developing important theories in molecular biology to the production of many types of vaccines. (wikipedia.org)
  • research
  • I'm trying to locate a rat neuroblastomal cell line for some research, but have not had much luck. (bio.net)
  • Useful research tool for the study of antigen processing and antigen presentation requirements of class I-restricted T cells. (nationaljewish.org)
  • A project is currently underway to enumerate and rename contaminated cell lines to avoid errors in research caused by misattribution (Masters, 2002). (wikipedia.org)
  • 2002). Contaminated cell lines have been extensively used in research without knowledge of their true character. (wikipedia.org)
  • For example, most if not all research on the endothelium ECV-304 or the megakaryocyte DAMI cell lines has in reality been conducted on bladder carcinoma and erythroleukemia cells, respectively. (wikipedia.org)
  • Thus, all research on endothelium- or megakaryocyte-specific functions utilizing these cell lines has turned out to be worthless, except as a warning example. (wikipedia.org)
  • It requires lengthy research to determine the precise point where cell lines have become contaminated. (wikipedia.org)
  • Experimental Cell Research. (wikipedia.org)
  • exhibit
  • So any cell line will exhibit this effect if there's enough LPS knocking around? (bio.net)
  • These cells exhibit routine endothelial functionality, such as, CD31 expression, AcLDL uptake and angiogenesis. (atcc.org)
  • tissue
  • The alternative, performing an analysis on primary cells from multiple tissue donors, does not have this advantage. (wikipedia.org)
  • commonly
  • They can restore the response of purified T lymphocytes to Con A. They show increased CO2 production on phagocytosis and can be differentiated into macrophage-like cells using for example phorbol 12-myristate 13-acetate (commonly known as PMA or TPA). (wikipedia.org)
  • normal
  • In particular how 'normal' are these cells metabolically? (bio.net)
  • An immortalized cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division. (wikipedia.org)