Lines neoplastic stem cells. Medical search. Frequent questions

An increased expression of MSI1 protein is observed in endometriosis and endometrial carcinoma siRNA-mediated inhibition of MSI expression in endometrial carcinoma cells induces apoptosis and inhibits cell proliferation by affecting the Notch signaling pathway MSI1 is highly expressed in neural progenitor cells and is required for normal development of the brain. (wikipedia.org)
Here we show that TGF-beta regulates proliferation, migration, and tumorigenicity of mesenchymal GBM cancer stem cells (CSCs) in vivo and in vitro. (uni-regensburg.de)
A phenotypic assessment showed significantly decreased animal survival, increased cancer stemness and invasion but decreased proliferation of the Inv cells in comparison to Core cells. (neurologycongress.com)
decreased cell proliferation, cell cycle arrest, and induction of apoptosis. (123dok.net)
It was suggested that HOXD9 contributes to both cell proliferation and/or cell survival. (123dok.net)
HOXD9 siRNA treatment of SK-MG-1 SP cells resulted in reduced cell proliferation. (123dok.net)
Conclusions: Our results suggest that HOXD9 may be a novel marker of GCSCs and cell proliferation and/or survival factor in gliomas and glioma cancer stem-like cells, and a potential therapeutic target. (123dok.net)
Homeobox proteins are master regulators of develop-ment and control many cellular processes, including proliferation, apoptosis, cell shape, and cell migration. (123dok.net)
Restitution of miR122 in BCLC9 cells, decreases cell proliferation rate and reduces significantly tumor size in vivo . (oncotarget.com)
Treatment of miR122 positive cells with an inhibitor of TGFBR1 activation, abolished tumor dormancy program and recovered cell proliferation rate through a Smad-independent TGF-β response. (oncotarget.com)
Mesenchymal stem cells (MSCs) have been widely used in regenerative medicine and clinical therapy due to their capabilities of proliferation, differentiation, and immune regulation. (techscience.com)
The myelodysplastic syndromes (MDS) are a group of clonal neoplastic hematologic disorders characterized by varying degrees of bone marrow failure, abnormal hematopoiesis, and proliferation of myeloid blast cells. (acgtfoundation.org)
JCI20679 suppresses the proliferation of glioblastoma stem cells by activating AMPK and decreasing NFATc2 expression levels. (addgene.org)
Retroviral vectors expressing short hairpin RNAs (shRNAs) against CDC2 caused efficient deletion of CDC2, cellular G2/M arrest concluding in apoptosis and inhibition of proliferation in human glioma cells U251 and SHG-44 cell lines ex vivo. (biomedcentral.com)
Mechanistically, EBF2 uniformly activates Pparγ-expression in pancreatic cancer cells and limits cell proliferation, whereas oncogenic drivers are expressed differentially in the progenitor cell subsets. (amegroups.org)
The complex PDAC pathobiology is established predominantly through KRAS mutations and the associated cellular signaling that contributes to cell proliferation and dedifferentiation ( 5 , 6 ). (amegroups.org)
Cell proliferation includes a series of events that is tightly regulated by several checkpoints and layers of control mechanisms. (frontiersin.org)
In conclusion, we provide useful experimental approaches and bioinformatics to identify informative and predictive genes at the single-cell level, which opens up new means to describe and understand cell proliferation and subpopulation dynamics. (frontiersin.org)
However, most of our knowledge about cell proliferation comes from studies that average data from large and mixed cell populations. (frontiersin.org)
indicated that ATMs colocalized with T cells in lymphoid clusters within adipose tissue and may act as APCs, which express high levels of MHCII and also costimulatory molecules and process and present antigens to induce CD4+ T-cell proliferation and activation in adipose tissue of obese mice (29, 68, 105). (exposed-skin-care.net)
showed that adipose tissue from obese mice induced proliferation of splenic CD8+ T cells, indicating a CD8+ T cell-activating environment in obese adipose tissue (31). (exposed-skin-care.net)
Recently, it was reported that miR-200a plays a crucial role in the development of cancer through its regulation of epithelial to mesenchymal transition (EMT), cell migration, proliferation and metastasis [ 11 , 12 , 13 ]. (oncotarget.com)
FLT3 ITD triggers the proliferation of the quiescent hematopoietic stem cell (HSC) pool but fails to directly transform HSCs. (biomedcentral.com)
Multiple myeloma is a B-cell-dependent hematological malignancy caused by excessive clonal proliferation of terminally differentiated plasma cells in bone marrow. (protheragen.com)

1 line of systemic therapy in case of participants ineligible for high-dose chemotherapy and autologous Hematopoietic stem cell transplantation (HSCT). (who.int)
Hematopoietic stem cell transplantation remains the only curative therapeutic approach that reliably results in resolution of bone marrow fibrosis in patients with myelofibrosis. (haematologica.org)
3 Myelofibrosis (MF) refers to the Philadelphia chromosome ( BCR-ABL1 )-negative myeloproliferative neoplasm (MPN) originating at the level of the multipotent hematopoietic stem cell. (haematologica.org)
Primary myelofibrosis results from neoplastic transformation of a pluripotent hematopoietic stem cell. (msdmanuals.com)
The Hematopoietic stem cell transplantation (HSCT) is used in children as a definitive treatment for various oncological, immune deficiencies, hemoglobinopathy, and malignancies diseases that involve the hematological system, congenital metabolism disorders, among others. (bvsalud.org)
Hematopoietic Stem Cell Transplantation. (bvsalud.org)
Hematopoietic stem cell transplantation not responded to other therapeutic modalities, with (HSCT) is used in children as a definitive treatment an increase in survival after transplantation, for different oncological, immune deficiencies, contributing to its use (YEILIPEK, 2014). (bvsalud.org)

The Hh pathway is an evolutionarily conserved system for regulating patterning and cell fate from Drosophila to humans. (biomedcentral.com)
In obese humans, adipocyte-secreted CCL20 may contribute to the deposition of Compact disc4+ helper and Compact disc8+ cytotoxic T lymphocytes within adipose tissues, possibly via connections with CCR6 which was upregulated on T cells in obese adipose tissues (100). (exposed-skin-care.net)

Through RT-PCR, Western Blot, flow cytometry, immunofluorescence, sphere formation xenograft tumor assays and drug resistance, the role of miR-17-5p and MKL-1 on promoting stem cell-like properties of gastric cancer were verified in vivo and vitro . (nih.gov)
Here we investigated the characteristics of a population of pancreatic cancer tumorspheres derived from different human pancreatic cancer cell lines and a primary line from a genetically engineered KPC mouse model, using flow cytometry and western blotting to analyse surface and stemness markers. (lu.se)
Materials and Methods: Solid HNSCC tumors, as well as permanent HNSCC cell lines, were analyzed by flow cytometry concerning the expression of different putative stem cell marker proteins. (uni-luebeck.de)
Western Blotting was used to determine the level of expression of CDC2 protein as measure to quantify down regulation of CDC2 expression along with use of flow cytometry to investigate effect of shRNAs on cell cycles and detection of apoptosis. (biomedcentral.com)

This study investigates whether UM progression is driven by a subpopulation of stem-like cells, termed "cancer stem cells" (CSCs). (whiterose.ac.uk)
called either cancer stem cells (CSCs) or tumor-initiat-ing cells[3]. (123dok.net)
It is known that GBM involves cancer stem-like cells (CSCs) and tumor cells that aggressively invade normal brain tissues, and both cell types may cause resistance to radiotherapy (RT) and are thus responsible for therapeutic failure. (tmu.edu.tw)
Cancer stem cells (CSCs) and poorly differentiated progenitor cells in pancreatic ductal adenocarcinoma (PDAC) exacerbate hierarchical tissue organization contributing to cell hyperproliferation and therapy resistance. (amegroups.org)
We isolated CSCs from low passage PDAC cell lines derived from human tumors and commercially available cell lines. (amegroups.org)
Cancer stem cells (CSCs) are characterized by their capacity for indefinite self-renew and by their relative quiescence [ 2 , 3 ]. (oncotarget.com)

Studies have increasingly focused on the potential therapeutic effects of stem cell transplantation for neurological diseases [ 10 ]. (hindawi.com)
this limitation has been overcome via ectopic expression of human telomerase reverse transcriptase (hTERT), the catalytic component of telomerase, to produce large quantities of these cells as an attractive source for cellular transplantation [ 16 - 18 ]. (hindawi.com)
Here we show that the brown adipose differentiation gene, EBF2, activates differentiation-associated signals in the cancer progenitor cells in culture and CSC transplantation models. (amegroups.org)
Treatment selection takes into account patient age, fitness, and whether autologous stem cell transplantation (ASCT) is planned. (medscape.com)
In patients who are eligible for autologous stem cell transplantation (ASCT), drugs are administered as induction and maintenance therapy. (protheragen.com)
Treatment is often supportive, but Janus kinase 2 ( JAK2 ) inhibitors, such as ruxolitinib , fedratinib , pacritnib, or momelotinib may decrease symptoms, and stem cell transplantation may be curative. (msdmanuals.com)

2 prior lines of anti-neoplastic systemic therapy. (who.int)
Metformin, a common oral biguanide used to treat type 2 diabetes, has been demonstrated to have anti-neoplastic effects. (weeksmd.com)

On a functional level, proneural CSC lines caused a significantly stronger TGF-beta-dependent suppression of NKG2D expression on CD8(+) T and NK cells in vitro providing a mechanistic explanation for the reduced immune infiltration of proneural GBMs. (uni-regensburg.de)
Proponents of stem cell research claim that the blastocyst is not human yet, and the embryos used for stem cell harvest are typically leftover from in vitro fertilization procedures with minimal chance that a human could ever develop from them. (nhsjs.com)
The controversy over embryonic stem cell research is caused by the fact that the procurement of these stem cells involves the destruction of the embryo produced during in vitro fertilization. (nhsjs.com)
Transcription factors associated with epithelial‐to‐mesenchymal transition (EMT) have been linked to this invasion, and we found that hypoxia increased in vitro invasion up to fourfold in glioblastoma neurosphere lines and induced the expression of ZEB1. (cardiff.ac.uk)
ZEB1 protein was also present in human fetal periventricular stem and progenitor cells and ZEB1 inhibition impaired migration of in vitro propagated human neural stem cells. (cardiff.ac.uk)
Although other irritants in the smoke may have contributed to the incident, there is supporting evidence that stable strontium can stimulate the release of histamine from mast cells in vitro (ATSDR 2001e). (cdc.gov)
Células cultivadas in vitro procedentes de tejido neoplásico que se utilizan en el estudio de las capacidades metabólicas y proliferativas de las células tumorales, en el pronóstico de las respuestas clínicas a la quimioterapia, en la detección de nuevos agentes antitumorales y en las investigaciones biológicas básicas. (bvsalud.org)
Cells grown in vitro from neoplastic tissue. (bvsalud.org)

It is a stem cell marker that controls the balance between self-renewal and terminal differentiation. (wikipedia.org)
Microarray profiling of Core and Inv cells for differences of expression of mRNAs and microRNAs resulted in the identification of differentially expressed gene targets controlling metabolic pathways, cell-cell and cell-matrix adhesion and cell differentiation. (neurologycongress.com)
miR122 is the prevalent miRNA in adult healthy liver and it is responsible for liver stem cell differentiation towards hepatocyte lineage. (oncotarget.com)
HCC stem-like cells can be directed towards cell differentiation and tumor dormancy by restoring miR122 expression. (oncotarget.com)
Precisely how mutant TET2 contributes to AITL oncogenesis is unclear, but experimental data has shown that hematopoietic stem cells (HSCs) are particularly vulnerable to disruption of TET2 function resulting in increased self-renewal capacity and altered terminal differentiation [ 18 ]. (biomedcentral.com)
Here we show that cell differentiation suppresses tumor formation. (amegroups.org)
Cells were transfected with the early B-cell factor-2 (EBF2) to activate progenitor cell differentiation and gauged the tumorigenic potentials of the derived cell subsets. (amegroups.org)
This study reveals a previously unrecognized function of EBF2 in regulating PDAC progenitor cell differentiation and tumor suppression. (amegroups.org)
We identified frequent mutations involving a 40kb distal repressor region for the key stem cell differentiation gene SOX2. (ox.ac.uk)
In order to be effective and integrated to the receiving area, the bone graft is required to allow a strong cell adhesion, interacting with several molecules to induce migration, differentiation, and thus the mineralization of the new bone on the graft. (bvsalud.org)
Notwithstanding, to achieve the effectiveness and integration of the grafted tissue to the receptor site, it is necessary a strong cell adhesion, so that it demands many molecules interaction to induce cell differentiation and the bone matrix mineralization formed onto the graft. (bvsalud.org)

Angioimmunoblastic T-cell lymphoma (AITL) is one of the most common subtypes of peripheral T-cell lymphoma. (biomedcentral.com)
B cell lymphoma 6 (Bcl6) is considered to be the lineage defining transcription factor for T follicular helper cells (T FH ) [ 20 ]. (biomedcentral.com)
Mantle cell lymphoma (MCL) is a lymphoproliferative disorder derived from a subset of naive pregerminal center cells localized in primary follicles or in the mantle region of secondary follicles. (medscape.com)
Mantle cell lymphoma (MCL) is recognized in the Revised European-American Lymphoma and World Health Organization classifications as a distinct clinicopathologic entity. (medscape.com)
it was frequently categorized as diffuse small-cleaved cell lymphoma (by the International Working Formulation) or centrocytic lymphoma (by the Kiel classification). (medscape.com)

Results: Distinct populations of CD44 expressing potential stem cells could be identified in solid tumors of HNSCC patients with strong individual deviations. (uni-luebeck.de)
Report shows how late-stage tumors can promote the development of 'suppressor' cells that prevent other immune cells from effectively fighting against the tumor. (hum-molgen.org)
Abnormalities in cell cycle regulation are reported to be strongly associated with tumorigenesis and progression of tumors. (biomedcentral.com)
Our aim was to establish a live cell culture to enable the understanding of the cellular processes driving the non-malignant growth of these tumors. (frontiersin.org)
Tumors are not simply a compilation of molecularly and phenotypically identical neoplastic cells. (lu.se)
Carcinoid tumors and small-cell carcinomas of the gallbladder and extrahepatic bile ducts: a comparative study based on 221 cases from the Surveillance, Epidemiology, and End Results Program. (cancercentrum.se)
This theory holds that tumors arise from 1 of 2 undifferentiated stem cells: the excretory duct reserve cell or the intercalated duct reserve cell. (medscape.com)

We then analyzed these 19 genes for their methylation pattern in pancreatic cancers and found that all 7 of the genes (claudin4, lipocalin2, 14-3-3σ, trefoil factor2, S100A4, mesothelin, and prostate stem cell antigen) that were overexpressed in the neoplastic cells of pancreatic cancers and not expressed in normal pancreatic duct displayed a high prevalence of hypomethylation in pancreatic cancer cell lines and primary pancreatic carcinomas. (johnshopkins.edu)
In pancreatic cancer cell lines that retained methylation of 1 or more of the 7 aforementioned overexpressed and hypomethylated genes, treatment with 5-aza-2′-deoxycytidine or with trichostatin A, either alone or in combination, almost invariably reactivated the transcription of each of these 7 genes. (johnshopkins.edu)
Background: HOX genes encode a family of homeodomain-containing transcription factors involved in the determination of cell fate and identity during embryonic development. (123dok.net)
BCLC9-miR122 cells down-regulate expression of MYC, KLF4, FOXM1, AKT2 and AKT3 genes and up-regulate FOXO1 and FOXO3A gene expression. (oncotarget.com)
The cell cycle is a complex process with myriad genes involved and elaborate and complex signaling mechanisms to allow for this critical cellular process. (biomedcentral.com)
Here, we used quantitative real-time PCR, profiling the expression of 93 genes in single-cells from three different cell lines. (frontiersin.org)
We found that the total transcript level per cell and the expression of most individual genes correlated with progression through the cell cycle, but not with cell size. (frontiersin.org)
Detailed analysis of cell cycle predictive genes allowed us to define subpopulations with distinct gene expression profiles and to calculate a cell cycle index that illustrates the transition of cells between cell cycle phases. (frontiersin.org)
Here, we employed single-cell gene expression profiling to describe the dynamic transition between cell proliferative states in three different cell lines using a panel consisting of 93 marker genes. (frontiersin.org)
Understanding the genes driving the inherited neoplastic susceptibility will lead to better patient- and cancer-specific management including choice of therapeutic regimen to prevent, or at least delay, development of myeloid neoplasia after treatment of a prior malignancy. (sahmri.org.au)

A novel spontaneous hepatocellular carcinoma mouse model for studying T-cell exhaustion in the tumor microenvironment. (addgene.org)
WSX1 act as a tumor suppressor in hepatocellular carcinoma by downregulating neoplastic PD-L1 expression. (addgene.org)
Novel Paired Cell Lines for the Study of Lipid Metabolism and Cancer Stemness of Hepatocellular Carcinoma. (addgene.org)

Although bone marrow fibrosis is seen in a variety of malignant and non-malignant disease states, the deposition of reticulin and collagen fibrosis in the bone marrow of patients with myelofibrosis is believed to be mediated by the myelofibrosis hematopoietic stem/progenitor cell, contributing to an impaired microenvironment favoring malignant over normal hematopoiesis. (haematologica.org)

Last, we show that established UM cell lines and UM STCs are plastic in nature and switch their phenotype in response to environmental stimuli. (whiterose.ac.uk)
In several types of cancer, side populations (SPs) have been shown to be enriched for cells with CSC-like activity and a CSC phenotype [ 4 ]. (oncotarget.com)

On one hand, it is clear that epigenetic alterations accumulate with age, leading to disrupted cell function and tissue pathology. (irbbarcelona.org)

Thought to be disorders of hematopoietic stem cells, clonal cytogenetic abnormalities are frequently identified. (acgtfoundation.org)
We discuss the rationale of various anti-fibrogenic treatment strategies targeting the clonal hematopoietic stem/progenitor cell, aberrant signaling pathways, fibrogenic cytokines, and the tumor microenvironment. (haematologica.org)
Recent Findings: Emerging evidence suggests that development of t-MN is the result of complex interactions including generation of somatic variants in hematopoietic stem cells and/or clonal selection pressure exerted by the DNA-damaging agents, and immune evasion on top of any inherited genetic susceptibility. (sahmri.org.au)
Overview of Myeloproliferative Neoplasms Myeloproliferative neoplasms are clonal proliferations of bone marrow hematopoietic stem cells, which can manifest as an increased number of functionally normal platelets, red blood cells (RBCs). (msdmanuals.com)

[ 2 ] Options for second-line therapy in patients with relapsed/refractory disease include chemotherapy-free regimens with biologic targeted agents such as covalent Bruton tyrosine kinase (BTK) inhibitors, lenalidomide,venetoclax, and chimeric antigen receptor (CAR) T-cell therapy. (medscape.com)
Activation of Typical T Cells in Adipose Tissues Compact disc4+ Purpureaside C T Cell Activation TCRs recognize the current presence of a particular antigen by binding to brief peptide sequences in the antigen that's shown on APCs. (exposed-skin-care.net)
These brief peptide sequences in the antigen are often presented over the cell surface area of APCs by using MHCII substances, which are necessary for activation of Compact disc4+ T cells (102). (exposed-skin-care.net)
Indeed, the principal adipocytes isolated from obese mice could induce antigen-specific Compact disc4+ T cell activation (58). (exposed-skin-care.net)
The proteasome plays an important role in the ubiquitin-protein pathway (UPP), which is a key pathway for intracellular protein degradation, regulation of antigen presentation, cell cycle, NF-kB metabolism, etc. (protheragen.com)
The multifunctional nuclear protein large T-antigen was detectable by immunohistochemical analyses in a subset of neoplastic cells. (cdc.gov)

Metformin, the first-line drug for treating diabetes, inhibits cellular transformation and selectively kills cancer stem cells in breast cancer cell lines. (weeksmd.com)
While the inflammatory transcription factor nuclear factor of activated T-cells 2 ( NFAT2 , NFATC1 ) is overexpressed in AML, it is unknown whether it plays a role in FLT3 ITD -induced HSC transformation. (biomedcentral.com)
We find that NFATC1 governs FLT3 ITD -driven precursor cell expansion and transformation, causing a fully penetrant lethal AML. (biomedcentral.com)
The primary myelofibrosis progeny cells stimulate bone marrow fibroblasts (which are not part of the neoplastic transformation) to produce excessive collagen. (msdmanuals.com)
PyV-induced tumorigenesis in laboratory animals, by simian virus 40 (SV40) or by multiple human PyVs, such as JC virus (JCV), is used extensively as a cell transformation model. (cdc.gov)
Thus, although PyV-induced oncogenesis in laboratory animals has been a prolific model for the study of the cell cycle and cell transformation, natural infections rarely result in tumor formation, so the steps in cell transformation after natural infection are being revealed more slowly. (cdc.gov)

abstract = "A cell line, designated RMG-I, has been established from ascites of a patient with ovarian clear cell carcinoma. (elsevierpure.com)

Furthermore, in order to clarify whether these 3 kinds of cells originate from one stem cell or not, monoclonal cell population were transplanted into nude mice, and its histological investigation revealed that 3 types of cells existed in the transplanted tumor, proving that they originate from one stem cell. (elsevierpure.com)
Another statement showed the preadipocyte- and endothelial cell-derived stromal-derived element-1 (CXCL12), mediated early infiltration of CD4+ T lymphocytes in obesity, which preceded the increase of macrophages in adipose cells of mice on HFD (101). (exposed-skin-care.net)

EMT is a process by which epithelial cells lose their cell-cell adhesion and gain invasive properties, which leads to the acquisition of mesenchymal stem cell characteristics [ 17 , 18 ]. (oncotarget.com)

However, obtaining primary neuronal cells from adult tissue is difficult and faces major ethical issues in clinical practice. (hindawi.com)
It is evident that some stem cell clones acquire somatic mutations that endow them with a self-renewal advantage, leading to their expansion and tissue-takeover in old age. (irbbarcelona.org)
Cells cultured as 3D were embedded as tissue blocks. (frontiersin.org)
The tumor is heterogeneous with meningeal tumor cells, mainly a mixture of fibroblast like cells, and neuroepithelial cells in a background of connective tissue. (frontiersin.org)
Hh proteins are secreted morphogens that play essential roles in regulation of embryogenesis, development, tissue homeostasis, regeneration and stem cell maintenance in a concentration-dependent manner [ 6 ]. (biomedcentral.com)
However, its role in aging-related adipose tissue CD4+ T cell activation remains to be investigated. (exposed-skin-care.net)
CD8+ T Cell Activation Compared to CD4+ T cells, CD8+ T cells show a greater increase in adipose tissue in obesity and in aging (31, 43, 106). (exposed-skin-care.net)
Similar to CD4+ T cells, CD8+ T cells exhibit effector memory or effector phenotypes expressing elevated levels of IFN- in obese adipose tissue (31, 44). (exposed-skin-care.net)
The mechanism for CD8+ T cell activation in adipose tissue is not fully understood. (exposed-skin-care.net)
Indeed, CD8+ T cells from mouse adipose tissue respond to cytokines and become activated and proliferate under stimulation of IL-12 and IL-18, which are mainly produced by APCs and are elevated in obese adipose tissue (44). (exposed-skin-care.net)
These tumorous epithelial cells are derived from luminal lactiferous ductal epithelium of the breast tissue (see the image below). (medscape.com)
Malignant Paget cells are derived from luminal lactiferous ductal epithelium (A) of breast tissue with retrograde extension of cancerous Paget cells into epidermis of overlying nipple (B). Enlarged circle shows details that reveal thickening both of lining epithelium of breast duct and of nipple skin. (medscape.com)
This paper is a systematic review of the literature on the mechanisms of cell adhesion to bone grafts associated to nanotechnology, describing the importance and the role of those molecules in the adhesion and thus in tissue regeneration. (bvsalud.org)
Accurate identification of these mechanisms of cell adhesion is essential for further advancement in tissue engineering, such as the production of biological bone substitutes that achieve a better clinical outcome. (bvsalud.org)
Tissue engineering is a strategy very used to obtain functional repairing through the development of biological substitutes that can restore, keep or substitute damaged tissues or organs 31 , through the combination of scaffolds biocompatible with live cells and/or bioactive molecules 19 . (bvsalud.org)
These properties will act mediating the action of cell adhesion proteins, regulating cell behavior and causing tissue regeneration. (bvsalud.org)

Effectively targeting cancer stem cells to treat cancer has great therapeutic prospects. (nih.gov)
Cancer stem cells are a population of slow-cycling cells within the tumour bulk, with self-renewal capacity that attracts interest as a therapeutic target. (lu.se)
Although next generation sequencing led to genomic landscaping of glioblastoma, there is little insight into the mechanism of neoplastic cell invasion, the detection of the invasive cells or their therapeutic targeting. (neurologycongress.com)

This conference will provide an interdisciplinary forum for researchers interested in aging, somatic mosaicism, epigenetics, plasticity, stem cells and immunity. (irbbarcelona.org)
Both 2D monolayer and 3D neurospheres were successfully cultured and characterized for proliferative potential, intrinsic plasticity, presence of cancer stem cells and the expression of stem cell markers. (frontiersin.org)

Background: Despite significant advances in the use of diagnosis and therapy to treat head and neck squamous cell carcinoma (HNSCC), the prognosis has improved only marginally in the last decades. (uni-luebeck.de)
Individual analysis of 'tumor stem cell' markers will be an important tool for innovative therapies and for determining the prognosis of patients with HNSCC. (uni-luebeck.de)
Advances in understanding the mutational landscape of AITL have not resulted in improved prognosis nor consensus regarding optimal first-line and second-line treatment. (biomedcentral.com)
Advances in understanding the mutational landscape of AITL have not resulted in improved prognosis over the last two decades (5-year overall survival rate 25-41%), nor consensus regarding optimal first-line and second-line treatment [ 3 ]. (biomedcentral.com)

ES are pluripotent, as they can transform into almost any human cell type. (nhsjs.com)
There are other, less controversial alternatives to embryonic stem cells, such as adult, fetal, cord blood, and induced pluripotent stem cells, but they have other biological restrictions that make them less promising for use in regenerative medicine at this time. (nhsjs.com)
Bone marrow stem cells, including the pluripotent hematopoietic stem cells (HSCs) and bone mesenchymal stem cells (BMSCs), are being considered as potential targets for cell and gene therapy-based approaches against a variety of different diseases. (hindawi.com)

12 Department of Neurosurgery and Center for Stem Cell & Regenerative Medicine, The Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston, Texas. (aacrjournals.org)

Methods: Expression of postulated stem cell markers aldehyde dehydrogenase (ALDH), CD44, and CD133 was analyzed in UM cell lines and primary UM short-term cultures (STCs) established from tumor samples. (whiterose.ac.uk)
Results: We demonstrate that expression of ALDH, CD44, and CD133 does not select for a subpopulation of stem-like cells in either UM cell lines or UM STCs. (whiterose.ac.uk)
Results: In this study, we found high expression of the HOXD9 gene transcript in glioma cell lines and human glioma tissues by quantitative real-time PCR. (123dok.net)
Finally, we cultured human glioma cancer stem cells (GCSCs) from patient specimens found with high expression of HOXD9 in GCSCs compared with normal astrocyte cells and neural stem/progenitor cells (NSPCs). (123dok.net)
In glioma CSC (GCSC), the expression of some neural stem markers, such as SOX2 and Musa-shi-1, has been reported[6,7]. (123dok.net)
We studied the effects of restoring miR122 expression in a distinctive cell line derived from human HCC-BCLC9 cells-with a solid stem-like cell profile, high tumor initiating ability and undetectable miR122 expression. (oncotarget.com)
We identified MSI1 expression to predict radioresistance through silencing an MSI1-high-expressing R2M2 cell line or inducing overexpression in a Par cell line with low/no MSI1 expression and assessing the subsequent DDR. (tmu.edu.tw)
Retroviral vectors expressing short hairpin RNAs (shRNAs) targeted against CDC2 were designed and transducted into human glioma cell line ex vivo in order to downregulate the expression of CDC2. (biomedcentral.com)
We evaluated cell stemness by using multiple functional assays in combination with stem cell marker expression analysis. (lu.se)
The functional and clinical significance of the identified mutations was examined using a combination of population-based whole genome sequencing, targeted deep sequencing, multi-center analysis of protein expression, loss of function experiments in an in-vivo reporter assay and mammalian models, and gain of function experiments in primary cultured fallopian tube epithelial (FTE) cells. (ox.ac.uk)
The purpose of this study was to examine the expression and function of the ATP-binding cassette protein ABCG2 and to characterize the cells for their expression of markers of Side Population (SP) progenitor cells and Stellate Cells (SC). (endocrine-abstracts.org)
further defined that mostly huge adipocytes from obese adipose tissues exhibited an increased expression degree of MHCII substances and acted as APCs to activate Compact disc4+ T cells to secrete IFN- (103). (exposed-skin-care.net)
Overexpression of miR-200a in SP cells decreased metastasis-related markers and expression of ZEB2. (oncotarget.com)
[ 6 ] reported on the immunohistochemical expression of apomucin MUC1, MUC2, and MUC5AC in PD and concluded that both epidermal Paget cells and underlying ductal carcinoma exhibit the same phenotypic apomucins that are also expressed by the Toker cells. (medscape.com)

Lastly, metformin-based combinatorial therapy is effective in xenografts involving inflammatory prostate and melanoma cell lines, whereas it is ineffective in noninflammatory cell lines from these lineages. (weeksmd.com)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
However, the main element substances that mediate T cell infiltration into adipose tissues in maturing remain to become discovered. (exposed-skin-care.net)
Abnormal myeloma cells produce a mutated form of immunoglobulin protein called M-protein, which accumulates in tissues and blood vessels. (protheragen.com)

Up to 12 adult participants with r/r aggressive B-cell NHL may be enrolled into a Run In dose level. (who.int)

The aim of this thesis was to evaluate how microenvironmental cues affect the interactions between the tumor microenvironment and glioma stem-like cells in glioblastoma. (lu.se)

These findings reveal new regulatory signaling pathways for gastric cancer stem cells, thus it give new insights on potential early diagnosis and/or molecular therapy for gastric cancer. (nih.gov)
Activation of the PI3 kinase-Akt pathways characterized the Inv cells, whereas activation of the ERK/MAP kinase pathway was specific to Core cells. (neurologycongress.com)
Two categories of targets potentially relevant for diagnostic and therapy were further addressed: enzymes involved in metabolic pathways and receptor molecules expressed at the plasma membrane of neoplastic cells. (neurologycongress.com)
FHND91 is 100% converted in aqueous solution to active molecules and results in tumor cell death through endoplasmic reticulum stress-dependent and mitochondria-mediated pathways. (protheragen.com)

The pathogenesis of mammary PD and the origin of Paget cells were once controversial. (medscape.com)
[ 5 ] Like Paget cells of both mammary and extramammary sites, Toker cells contain prominent clear (vacuolated) cytoplasm, and they are considered benign counterparts of Paget cells. (medscape.com)
Mammary Paget cells are malignant epithelial cells derived from underlying ductal adenocarcinoma of the breast that invade into the skin of nipple and areolar areas. (medscape.com)
A concept of a "collision" between the neoplastic lesion of mammary PD and the underlying carcinoma is therefore presented. (medscape.com)

To reveal the roles of CDC2/Cyclin B1 in human malignant gliomas, CDC2 in glioma cell line, it was down-regulated by retrovirus vectors expressing short hairpin RNAs (shRNAs) ex vivo and in vivo. (biomedcentral.com)

Musashi-1 (MSI1) is a neural stem-cell marker and key oncogenic factor of GBM. (tmu.edu.tw)
Normally, Hh ligand secreted by the notochord induces the ventral cell fate specification in the entire neural tube. (biomedcentral.com)

Furthermore, there is an absence of a cellular hierarchy in cell lines and all cells in culture are able to drive tumor progression. (whiterose.ac.uk)
The identity of these cells and the underlying cellular and molecular mechanisms are mostly unknown in HNSCC to date. (uni-luebeck.de)
Cellular dormancy and heterogeneity in cell cycle length provide important explanations for treatment failure after adjuvant therapy with S-phase cytotoxics in colorectal cancer (CRC), yet the molecular control of the dormant versus cycling state remains unknown. (ox.ac.uk)

There are many types of stem cells, but the one with the most potential is the embryonic stem (ES) cell. (nhsjs.com)
However, the removal of embryonic stem cells destroys the early embryo. (nhsjs.com)
Considering the great potential of embryonic stem cell research, it is argued here that their research be allowed to be legal, federally funded, and its development a national priority. (nhsjs.com)
There are many types of stem cells, but most of the controversy surrounds embryonic stem cells, as they are derived from human embryos. (nhsjs.com)
Embryonic stem cells are totally undifferentiated and have the ability to divide and create multiple specialized cell types. (nhsjs.com)
In the United States currently embryonic stem cell research is allowed but there has been a lot of public controversy and legal setbacks. (nhsjs.com)
This newer bill calls for prioritizing federally assisted advancement of embryonic stem cell research ( 1). (nhsjs.com)
Additionally, there have been two executive orders focusing on embryonic stem cells, one released by President George W. Bush prohibiting embryonic stem cell research and related federal funding, the other by President Barack Obama reversing the previous order but still with restrictions in place ( 2). (nhsjs.com)
Indeed, the potent pathotropic migratory properties of BMSCs and ability to circumvent both the complications associated with immune rejection of allogenic cells and many of the moral reasons associated with embryonic stem cell use suggest that BMSCs are most promising stem cells as a potential target for the clinical use of genetically engineered stem cells [ 14 , 15 ]. (hindawi.com)

The use of human telomerase reverse transcriptase-immortalized bone marrow mesenchymal stromal cells (hTERT-BMSCs) as vehicles to deliver antinociceptive galanin (GAL) molecules into pain-processing centers represents a novel cell therapy strategy for pain management. (hindawi.com)
The characteristic features of myeloma include increased plasma cells in the bone marrow and extramedullary sites, elevated monoclonal M-protein in serum and urine, osteolytic bone lesions, renal insufficiency, anemia, and immunodeficiency. (protheragen.com)
After the use of search strategies, 18 articles that describe processes of cell adhesion to bone grafts were selected. (bvsalud.org)
Several molecules are involved in the process of cell adhesion to bone grafts, highlighting the role of integrins, the focal adhesion mechanism, the influence of the collagen matrix, and the activity of alkaline phosphatase in bone matrix formation. (bvsalud.org)
Primary myelofibrosis (PMF) is a chronic myeloproliferative neoplasm characterized by bone marrow fibrosis, splenomegaly, and anemia with nucleated and teardrop-shaped red blood cells. (msdmanuals.com)

Diffuse spread through brain parenchyma and the presence of hypoxic foci rimmed by neoplastic cells are two cardinal features of glioblastoma, and low oxygen is thought to drive movement of malignant gliomas in the core of the lesions. (cardiff.ac.uk)
In malignant cells, UPP is often overexpressed due to excessive degradation needs caused by too many misfolded proteins. (protheragen.com)
By thorough histologic examination, Muir documented intraepidermal extension of malignant ductal epithelial cells through the lactiferous ducts and ductules into the epidermis (epidermotropism). (medscape.com)
Malignant epithelial (Paget) cells infiltrate and proliferate in the epidermis, causing thickening of the nipple and the areolar skin. (medscape.com)
Overview of Leukemia Leukemia is a malignant condition involving the excess production of immature or abnormal leukocytes, which eventually suppresses the production of normal blood cells and results in symptoms. (msdmanuals.com)

Itraconazole targets cell cycle heterogeneity in colorectal cancer. (ox.ac.uk)
Most studies have been performed on large cell populations, but detailed understanding of cell dynamics and heterogeneity requires single-cell analysis. (frontiersin.org)

Using a cancer stem-cell like human glioblastoma cell line in an orthotopic model, we isolated pairs of neoplastic cells growing either in the core of the tumor (Core cells) or away for the core, in the opposite hemisphere (Inv cells). (neurologycongress.com)
For this work, we used genetically engineered mouse models of glioma, primary stromal and glioma cell lines, classical glioblastoma cell lines, and organotypic slice cultures. (lu.se)

In highly heterogeneous tumours, like pancreatic ductal adenocarcinoma (PDAC) however, the characterisation of cancer stem cells has led to controversial results due to the lack of consensus on specific markers. (lu.se)

Up to 70% of hepatocellular carcinomas (HCC) show miR122 down-regulation [ 6 ] and the same applies for HCC-derived cell lines [ 7 ]. (oncotarget.com)
Recent advances have been made by an accumulation of studies on Merkel cell polyomavirus (MCPyV), which is highly associated and integrated in most Merkel cell carcinomas ( 5 ). (cdc.gov)
Excretory stem cells give rise to squamous cell and mucoepidermoid carcinomas, while intercalated stem cells give rise to pleomorphic adenomas, oncocytomas, adenoid cystic carcinomas, adenocarcinomas, and acinic cell carcinomas. (medscape.com)
Squamous cell carcinomas arise from excretory duct cells, pleomorphic adenomas arise from the intercalated duct cells, oncocytomas arise from the striated duct cells, and acinic cell carcinomas arise from acinar cells. (medscape.com)

It is becoming increasingly obvious that stem cells play an important role in tumor development and progression. (uni-luebeck.de)
Conclusion: CD44+ 'tumor stem cells' may play a key role in the establishment of permanent HNSCC cell lines, selecting especially robust cell entities that might drive the progression and metastasis of HNSCC. (uni-luebeck.de)
The molecular processes behind cell cycle progression have been dissected by numerous morphological studies on live or fixed single cells using a plethora of techniques to visualize components and processes during cell division. (frontiersin.org)

We have developed a genetically modified tumor cell vaccine for the treatment of myeloid malignancies. (acgtfoundation.org)
They suggested that the epidermal Paget cells are genetically different from those of breast carcinoma. (medscape.com)

Preclinical validation shows itraconazole to be effective in multiple assays through Wnt inhibition, causing both cycling and dormant cells to switch to global senescence. (ox.ac.uk)
Previous studies have indicated that cancer cells rely heavily on proteasomes to meet the demand for turnover and are therefore more sensitive to proteasomal inhibition than normal cells. (protheragen.com)

Although studies have identified unique transcription factors in the selection of normal pancreatic lineages, the cells of origin and molecular mechanisms arbitrating PDAC growth are poorly defined. (amegroups.org)

Although human HSCs as vehicles to treat metachromatic leukodystrophy (MLD) has been used to treat patients with early onset MLD in a phase I/II trial, the HSCs give rise to all different blood cell lineages, such as the myeloid and lymphoid cell lineages [ 11 ]. (hindawi.com)
K562 cells express many of the antigens shown to be overexpressed in myeloid leukemias and MDS. (acgtfoundation.org)
Studies are needed to better define an individual's inherited neoplastic susceptibility which will help predict the risk of myeloid neoplasia in the future. (sahmri.org.au)

Many previous reports have indicated that microRNA and EMT regulated cancer stem cell characteristics, and miR-17 and MKL-1 were involved as a critical gene in migration and invasion in the EMT pathway. (nih.gov)
One potential target is the cancer stem cell (CSC). (weeksmd.com)

Researchers reveal a fresh three-dimensional look at the spike proteins on HIV's coating that allow it to bind and fuse with human cells. (hum-molgen.org)

Such cells are derived from human embryos, and are undifferentiated, unlike other specialized cells in the human body. (nhsjs.com)
Adversaries of stem cell research argue that embryos are human and destroying one is equal to murdering a child. (nhsjs.com)
When the stem cells divide, the new cell may remain a stem cell to divide again or may differentiate to become any of the 200 types of specialized cells in the human body. (nhsjs.com)
Establishment of a human ovarian clear cell carcinoma cell line (RMG-I) and its single cell cloning--with special reference to the stem cell of the tumor. (elsevierpure.com)
Functional analysis of HOXD9 in human gliomas and glioma cancer stem cells. (123dok.net)
The human erythroleukemia cell line K562 has been stably transfected to secrete GM-CSF. (acgtfoundation.org)
Downregulation of CDC2 could potentialy inhibit human gliomas cells growth ex vivo and in vivo. (biomedcentral.com)
Human CRC cell dormancy and tumor growth can also be perturbed by itraconazole, which is found to inhibit Wnt signaling through noncanonical hedgehog signaling. (ox.ac.uk)
Today's research was performed to explore the immunomodulatory actions of two such cytokines, changing growth element- (TGF-) and interleukin-10 (IL-10), both which are aberrantly made by human being pancreatic carcinoma cells (this research). (academiaperuanadepsicologia.org)
Cell Lines and MK-6096 (Filorexant) CM Human being pancreatic carcinoma cell lines Capan2 (American Type Tradition Collection (ATCC), Rockville, MD), PT45, and BxPC3 supplied by Dr (kindly. (academiaperuanadepsicologia.org)
Recent studies have suggested that SP cells in human pancreatic cancer are characteristically chemoresistant [ 5 ]. (oncotarget.com)

Anatomy11

Chemicals and Drugs1

Analytical, Diagnostic and Therapeutic Techniques and Equipment3

Phenomena and Processes2

Proliferation24

Hematopoietic stem7

Humans2

Flow cytometry4

CSCs6

Transplantation6

Anti-neoplastic2

Vitro8

Differentiation11

Lymphoma5

Tumors7

Genes10

Hepatocellular carcinoma3

Hematopoiesis1

Phenotype2

Pathology1

Clonal4

Antigen6

Transformation6

Abstract1

Mice2

Mesenchymal stem1

Tissue16

Therapeutic3

Plasticity2

Prognosis4

Pluripotent3

Regenerative medicine1

Expression14

Melanoma1

Tissues3

Adult1

Tumor microenvironment1

Pathways4

Mammary4

Glioma cell line1

Neural2

Cellular3

Embryonic9

Bone5

Malignant5

Heterogeneity2

Glioblastoma cell2

Markers1

Carcinomas4

Progression3

Genetically2

Inhibition2

Transcription factors1

Myeloid3

Cancer stem2

Proteins1

Human11