Line cells. Medical search. Frequent questions

Precursor cells and their phenotypes during the differentiation of hematopoietic stem cells towards immature B cells. (uni-potsdam.de)

Robust F1 hybrid embryonic stem cell line from Mus musculus (lab mouse), generated by crossing C56BL/6J mice to Black-Swiss mice. (seattlechildrens.org)
Researchers make hiPSCs by harvesting somatic cells-often from skin-from a person and then reprogramming them to enter an embryonic-like state. (the-scientist.com)
We tested 273 hepatotoxic, 191 cardiotoxic, and 85 nephrotoxic compounds in HepG2 (hepatocellular carcinoma), H9c2 (embryonic myocardium), and NRK-52E (kidney proximal tubule) cells for their cytotoxicity. (nih.gov)
Researchers at Johns Hopkins have established a human cell-based system for studying sickle cell anemia by reprogramming somatic cells to an embryonic stem cell like state. (hopkinsmedicine.org)
The research team first sought to improve previously established methods for reprogramming of adult cells into so-called induced pluripotent stem (iPS) cells, which look and behave similarly to embryonic stem cells and can differentiate into many different cell types. (hopkinsmedicine.org)
Without large T, cells form embryonic stem cell-like clusters in three to four weeks. (hopkinsmedicine.org)
With large T, the cells started looking like embryonic stem cells in just 12 to 14 days. (hopkinsmedicine.org)
Not only did T speed up reprogramming, we also found that it increases the total number of reprogrammed cells, which is great because often in reprogramming, not all cells go all the way," says Cheng, who explains that rigorous follow-up tests are required to determine if the reprogrammed cells really behave like pluripotent embryonic stem cells. (hopkinsmedicine.org)
Embryonic stem cell-like clusters were visible 14 days after they initiated reprogramming and from these clusters the researchers established three different cell lines that both look and behave like human embryonic stem cells. (hopkinsmedicine.org)
A targeted neuroglial reporter line generated by homologous recombination in human embryonic stem cells. (ca.gov)
In this study, we targeted Olig2, a basic helix-loop-helix transcription factor that plays an important role in motoneuron and oligodendrocyte development, in human embryonic stem cell (hESC) line BG01 by homologous recombination. (ca.gov)
Clinical research had demonstrated the therapeutic benefits of interventions using adult stem cells rather than embryonic cells, a direction that guaranteed respect for human dignity, even at the embryonic stage. (who.int)

EBV infectability and permanent conversion into EBV positive sub-lines is possible by in vitro infection. (sigmaaldrich.com)
These new agreements will enable us to offer our clients expanded and prioritized cell line availability and supply," CrownBio CSO Henry Li said in a statement, adding that the deals ensure CrownBio's customers have access to "a range of in vitro and in vivo models for drug discovery. (genomeweb.com)
Researchers from South Korea and Switzerland have developed magnetically actuated microrobots and used them for precise stem cell delivery and transplantation in vitro , ex vivo and in vivo . (physicsworld.com)
In our current project we are working on the generation of immortal immune cell lines in order to improve our In vitro Immunization approach. (uni-potsdam.de)
Thereby the in vitro Immunization represents an alternative to the conventional hybridoma technique because cell lines would provide permanent and limitless numbers of the immune cells needed and could be generated from different host species. (uni-potsdam.de)
First steps in this direction are the characterization of relevant immune cells, optimization of culture conditions and in vitro differentiation from precursor to mature immune cells. (uni-potsdam.de)
Objective: Serum is usually added to growth media when mammalian cells are cultured in vitro to supply the cells with growth factors, hormones, nutrients and trace elements. (lu.se)
In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)

Wild-type cell lines are sold with knockout cell lines only - not available for individual purchase. (abcam.com)
EdiGene has the industry's largest off-the shelf diploid library of over 2,800 single clonal knockout (KO) cell lines, targeting over 2,600 genes in commonly used human cancer cell lines such as HeLa, HEK293-T, A549, HCT116, Hep G2 and MCF7. (pharmatimes.com)
To support your research on the role and mechanism of SUMO modifications, Horizon has developed a collection of human knockout HAP1 cell lines that lack components of the SUMOylation pathway. (horizondiscovery.com)
Choose from over 300 knock-in and knockout cell line models in many standard cancer cell lines such as DLD1, MCF10A, and HCT116. (horizondiscovery.com)

The agreement with the NCI grants CrownBio access to 100 cell lines available through the NCI Repository of Tumors and Tumor Cell Lines, and other sources that were developed in NCI labs, the company said. (genomeweb.com)
Evidence suggests that up to a third of established tumor cell lines being used for research are affected by inter- or intra-species cross-contamination or have been wrongly identified. (labmanager.com)
Large libraries of cancer cell lines - collections of cells that represent tumor types seen in cancer patients - can yield profound insights into tumors' unique genetic features and their sensitivities to current and potential treatments. (broadinstitute.org)

One is the DepMap project, an effort being undertaken at the Broad Institute and at the Sanger Institute to systematically identify genetic dependencies (vulnerabilities that might serve as targets for designing new therapies or repurposing existing ones) across hundreds of cancer cell lines using RNA interference, CRISPR, and drug screens. (broadinstitute.org)

The results, published in the Journal of Functional Foods, show that strawberry tree honey is capable of inhibiting the proliferation of tumour cells cultivated in laboratory plates - more specifically, those of a line of human colon adenocarcinoma (HCT-116) and another with metastatic characteristics (LoVo), widely used to investigate this type of tumour. (agenciasinc.es)
Strawberry tree honey is capable of inhibiting the proliferation of tumour cells on a line of human colon adenocarcinoma (HCT-116) and another with metastatic characteristics (LoVo). (agenciasinc.es)
The RET kinase inhibitor selpercatinib (Retevmo) more than doubled progression-free survival (PFS) compared with standard chemotherapy with or without pembrolizumab (Keytruda) in the first-line setting among patients with RET fusion advanced/metastatic non-small cell lung cancer (NSCLC). (medscape.com)
Enrolment of older adults with advanced or metastatic non-small cell lung cancer in first-line clinical trials in the multicentre ESME cohort. (bvsalud.org)
Inclusion criteria were patients with advanced or metastatic non-small cell lung cancer (AMNSCLC) aged 70 years or older, with at least one line of systemic treatment from 01 January 2015 to 31 December 2018. (bvsalud.org)

Large cancer cell line collections broadly capture the genomic diversity of human cancers and provide valuable insight into anti-cancer drug response. (nature.com)
The first human, trophoblastic endocrine cell type to be maintained in continuous culture. (sigmaaldrich.com)
Human BeWo cell line has been used to study the effects of Δ-9-tetrahydrocannabinol (Δ9-THC) on the proliferation and gene transcription in the BeWo trophoblast cell line. (sigmaaldrich.com)
RAMOS Cell Line human has been used to carry out a MTT (colorimetric assay) cell viability assay to measure calcium ionophore-induced cell death in Ramos cells under different conditions and to determine the expression of ZFP36 ring finger protein like 1 (ZFP36L1) in Ramos B cells during different stages of B cell differentiation. (sigmaaldrich.com)
A new partnership opens the door for a source of highly purified human primary cells and defined cell culture media. (the-scientist.com)
Most of the human induced pluripotent stem cells stored at major cell line repositories and used in research harbor thousands of DNA errors, a study finds, highlighting the need for improved quality control measures. (the-scientist.com)
S tem cell research that uses pluripotent stem cells derived from human skin or blood cells has led to numerous discoveries, aided drug development , and proven useful in gene therapies. (the-scientist.com)
However, many of these human induced pluripotent stem cell (hiPSC) lines banked in repositories or developed in labs likely harbor thousands of undetected mutations, casting doubt on how generalizable the findings made with them can be, according to research published yesterday (August 11) in Nature Genetics . (the-scientist.com)
Further analysis revealed that mutations, especially the BCOR mutations in the blood-derived lines, can occur after reprogramming, meaning they didn't originate from the human donors but rather arose as the cells replicated in the lab, likely through selective pressures the cells experience while growing and dividing in a dish. (the-scientist.com)
Prolor Biotech obtained a nonexclusive license to The University of Queensland's (UQ) human growth hormone (HGH) receptor cell line. (genengnews.com)
SK-MG-09 is a human brain cancer cell line. (mskcc.org)
DOI=10.1073/pnas.81.2.568 Mattes M.J., Cordon-Cardo C., Lewis J.L. Jr., Old L.J., Lloyd K.O.Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.Proc. (mskcc.org)
DOI=10.1073/pnas.79.18.5641 Cairncross J.G., Mattes M.J., Beresford H.R., Albino A.P., Houghton A.N., Lloyd K.O., Old L.J. Cell surface antigens of human astrocytoma defined by mouse monoclonal antibodies: identification of astrocytoma subsets. (mskcc.org)
Next, Jeon and colleagues established the feasibility of targeted cell delivery using microrobots loaded with human colon cancer cells in a body-on-a-chip microfluidic cell culture platform that mimics parts of a liver tumour network. (physicsworld.com)
They cultured mesenchymal stem cells derived from the human nose on spherical microrobots for transplantation in the mouse, and showed that an externally applied magnetic field could manipulate the cell-loaded microrobots within the animal's hollow abdominal cavity. (physicsworld.com)
For decades, scientists have used human cells that come from elective abortions to make vaccines such as rubella and chicken pox. (abc.net.au)
There are strong ethical regulations surrounding the use of any human cell, particularly foetal human cells,' Dr Coatsworth said. (abc.net.au)
Using both fetal and adult human skin cells, the researchers introduced the four genes previously reported sufficient for cell reprogramming and compared the efficiency of reprogramming in the presence or absence of large T antigen. (hopkinsmedicine.org)
Having established a faster, more efficient method, the team then reprogrammed human cells that contain the mutation associated with sickle cell anemia. (hopkinsmedicine.org)
This strategy was used to synthesize a series of spiro-bicyclic compounds designed to be paclitaxel mimetics, which were evaluated in human breast-derived cell lines. (lu.se)
The HepG2 cell line was transiently transfected with CYP2B6- and CYP3A4-luciferase promoter reporter plasmids along with human PXR (hPXR) or hCAR expression vectors. (cdc.gov)
Dr CHAUHAN (India)1 welcomed the updated WHO Guiding Principles on Human Cell, Tissue and Organ Transplantation. (who.int)

I have a small query on NCI60 cell line expression with specific target gene. (google.com)
The cell line market is in the early stages of development as a research tool, where the provision of more choice, with the right gene targets in the right cell lines, will allow the market to rapidly expand. (pharmatimes.com)
In 2012, the CCLE collaborators took a deep dive into the genomic features and drug sensitivities of these cells, cataloging gene expression, chromosomal copy number, and targeted gene sequencing data from all 947 lines and a number of drug-response profiles. (broadinstitute.org)
The present work reports an hESC reporter line generated by homologous recombination targeting a neural lineage-specific gene, which can be differentiated and sorted to obtain pure neural progenitor populations. (ca.gov)
In CAR T cell therapy, doctors remove a patient's T cells, implant a gene in them and infuse them back into the patient. (lymphomainfo.net)
Materials and Methods: We have adapted the Burkitt's lymphoma line Ramos to a serum-free medium that supports long-term survival and studied gene expression changes that occurred during the adaptation process. (lu.se)

Only approximately 5% of compounds showed differential toxicity responses in the cell lines with no obvious correlation to the known in vivo organ toxicity. (nih.gov)
Finally, the team demonstrated in vivo stem cell transportation using the microrobots in a live nude mouse. (physicsworld.com)
The use of such cell lines allows the ex vivo induction of an immune response and makes it independent from experimental animals. (uni-potsdam.de)
None of these effects was observed when honey was applied to healthy cell lines," the authors point out, noting the anticarcinogenic potential of this natural product, but insisting on the need for new studies with colon cancer in vivo models to confirm its chemopreventive effects. (agenciasinc.es)

Not only can the cell line measure the hormone's potency, but it can also be used to detect neutralizing antibodies directed against HGH," Novik points out. (genengnews.com)
Cheri Walker, SVP of corporate development at Abcam, said that the company is "excited to be able to bring the same level of industry-leading quality and data to the cell lines market as we have to research antibodies. (pharmatimes.com)

Stem cells derived from adult somatic cells "will carry the mutational history of their past, as well as of any new mutations that occur when you are reprogramming them or growing them in culture," Nik-Zainal says. (the-scientist.com)

In order to deposit a cell line someplace like HipSci, researchers only have to demonstrate that the stem cells don't have any missing or duplicated chromosomes or other largescale genetic errors-analyses that would miss the myriad single-nucleotide mutations identified in the new paper. (the-scientist.com)
Precise delivery of stem cells to the desired target is, however, crucial for the success of transplantation. (physicsworld.com)
Fabricated by 3D laser lithography, the microrobots' porous structure facilitates the attachment, proliferation and differentiation of various stem cells. (physicsworld.com)
Upon application of a rotating magnetic field, the spherical and helical microrobots respectively exhibited rolling and corkscrew motions that propelled them through 3D spaces - such as a blood vessel isolated from a rat brain and a ventricle within a slice of mouse brain - enabling precise targeting of transplanted stem cells. (physicsworld.com)
The researchers examined hippocampal neural stem cells (NSCs) grown on the microrobots. (physicsworld.com)
Fluorescent microscopy images of neural stem cells and their differentiation into astrocytes, oligodendrocytes and neurons on a lab dish, a helical microrobot and a spherical microrobot. (physicsworld.com)
Publishing online in Stem Cells on May 29, the team describes a faster and more efficient method of reprogramming cells that might speed the development of stem cell therapies. (hopkinsmedicine.org)
One challenge to studying blood diseases like sickle cell anemia is that blood stem cells can't be kept alive for very long in the lab, so researchers need to keep returning to patients for more cells to study," says Cheng. (hopkinsmedicine.org)
The proliferation and differentiation of pluripotent stem cells give rise to progeny that can populate the entire immunologic and hematopoietic systems through committed progenitors of both the lymphoid and myeloid lineages. (medscape.com)

Cellular differentiation of this stem cell type occurs when hormonal precursors are added to the medium. (sigmaaldrich.com)

A) Twenty-four hours after infection, PEDV nucleoprotein in infected cells was detected by immunofluorescence assay using fluorescein isothiocyanate- labeled nucleoprotein-specific monoclonal antibody. (cdc.gov)
Cytotoxicity was assessed using the MTT assay (cell viability) and Griess reagent (NO release). (bvsalud.org)

The lysate range is expected to be available through the Abcam website in early Q4 2019, with the expanded cell line offering following throughout 2020. (pharmatimes.com)

Here we show substantial agreement and biological consilience between drug sensitivity measurements and their associated genomic predictors from two publicly available large-scale pharmacogenomics resources: The Cancer Cell Line Encyclopedia and the Genomics of Drug Sensitivity in Cancer databases. (nature.com)
Systematic identification of genomic markers of drug sensitivity in cancer cells. (nature.com)
We assess whether drug sensitivity in cancer cell lines is able to discriminate tissue specificity. (nih.gov)

A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes. (nature.com)
Even when considering distinct cancer subtypes and targeted therapies, most drugs are evenly effective/ineffective throughout all cell lines. (nih.gov)

The Salk professor developed the institute's first neurobiology lab and used it to develop cell lines, describe amyloid β toxicity, and screen for compounds that protect against neurodegeneration. (the-scientist.com)
Our results suggest that from a general screening perspective, different cell lines have relatively equal value in assessing general cytotoxicity and that specific organ toxicity cannot be accurately predicted using such a simple approach. (nih.gov)
Select organ toxicity potentially results from compound accumulation in a particular tissue, cell types within organs, metabolism, and off-target effects. (nih.gov)
Synthesis and toxicity evaluation in breast cancer cell lines. (lu.se)

The U.S. Food and Drug Administration (FDA) approved two CAR T-cell therapies in 2017, Kymriah developed by Novartis for the treatment of children with acute lymphoblastic leukemia and Yescarta developed by Kite for adults with advanced lymphomas. (nature.com)
It also suppresses the receptor of the epidermal growth factor called EGFR/HER2 and its signalling pathways, which could be an attractive target in cancer therapies because of their important role in the processes of cell survival and proliferation, as well as in those of apoptosis and metastasis. (agenciasinc.es)

For 75 cm 2 flasks, use approximately 10 5 cells/flask and subculture every 3-4 days. (neb.com)

A cell line can harbor thousands of mutations and still be usable for research as long as those mutations are concentrated in irrelevant noncoding areas or don't hit important genes, Nik-Zainal says. (the-scientist.com)
Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
Treatment with honey stops the tumour cells cycle by regulating some genes (cyclin D1, CDK2, p27Kip. (agenciasinc.es)
it inhibits cell migration, reduces the ability to form colonies and induces apoptosis or programmed cell death through the modulation of other key genes (p53, caspase-3, c-PARP) and various apoptotic factors," says Maurizio Battino, coordinator of the research. (agenciasinc.es)
Most changes were subtle, but frequently the genes with altered expression were involved in basal cellular functions such as cell division, cell cycle regulation, apoptosis and cell signalling. (lu.se)
Some alterations were restored when the cells were transferred back to serum-containing medium, indicating that expression of these genes was controlled by components in serum. (lu.se)
The precise pathophysiology of inherited single cell and multilineage cytopenias has not been elucidated despite the identification of many of the genes mutated in these disorders. (medscape.com)

Accelerated hematopoietic cell apoptosis has been demonstrated in virtually all IBMFS. (medscape.com)

There is a demand for DMSO-free cryoprotectants that maintain cell viability and function after thaw. (nature.com)

Abcam has entered the cell editing market through acquisition of the entire live cell line and lysates portfolio of EdiGene Inc, a company focused on developing genome editing technologies into novel therapeutics for a broad range of diseases and into creative solutions to advance drug discovery. (pharmatimes.com)

Chinese Hamster Ovary (CHO) cell lines are preferred host cells for therapeutic protein production. (nih.gov)
A multi-center research team has now greatly augmented this cancer research resource by incorporating new cell lines and adding new data spanning the molecular spectrum from sequence to expression to protein. (broadinstitute.org)
Flp-In™ 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In™ expression vector. (thermofisher.com)

Researchers need to start with a reliable process for commercial biopharmaceutical production in living cells. (the-scientist.com)
Despite the varied causes of irreproducibility from factors that are difficult to control, cell line authentication (CLA) is a relatively simple operation that assures that the cells researchers believe they are working with are actually those cells, and that they are contaminant-free. (labmanager.com)
Researchers take a broad, multi-faceted look at a large collection of cancer cell lines, creating a new trove of data to explore for drug development opportunities. (broadinstitute.org)
Spanish and Italian researchers have proven that when honey from strawberry trees, a product typical of Mediterranean areas, is added to colon cancer cells grown in the laboratory, cell proliferation is stopped. (agenciasinc.es)

Cell Factory engineers contribute to the transition towards a sustainable society by supplying genetically engineered cell factories for fermentation-based production of proteins or biochemicals. (dtu.dk)
The genetically engineered T cells are then awakened to go after the cancer. (lymphomainfo.net)

immortalized duck intestinal epithelial cell (MK-DIEC) line, which was generated from the intestinal tissues of Mahesh Khatri a 19- day-old white Pekin duck embryo. (cdc.gov)
We find that cancer-specific drugs do not show higher efficacies in cell lines representing the respective tissues. (nih.gov)
Stem cell therapy is an emerging method for restoring damaged tissues or treating a variety of diseases, from cancer to degenerative neural disorders like Alzheimer's disease. (physicsworld.com)
Tissues and Cells. (lu.se)

The second is PRISM , a system that uses genetically-barcoded versions of the CCLE cell lines to identify biomarkers that could be used to predict tumors' responses to different drug compounds. (broadinstitute.org)

Chimeric antigen receptor (CAR) T-cell therapy is a rapidly growing therapy for the treatment of cancer 1 . (nature.com)
BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity. (nature.com)
NEW YORK - Crown Bioscience said on Thursday that it has signed separate five-year portfolio license agreements with the National Cancer Institute and the nonprofit biorepository organization ATCC that will grant the company access to the institutions' cell lines. (genomeweb.com)
Cancer cell lines have a prominent role in the initial stages of drug discovery, facilitating high-throughput screening of potential drugs. (nih.gov)
Such is the case with the Cancer Cell Line Encyclopedia (CCLE) , a collection of more than 900 cell lines assembled starting in 2008 by the Broad Cancer Program in collaboration with the Novartis Institutes for BioMedical Research. (broadinstitute.org)
and WRN in cancer cells lacking a key DNA proofreading mechanism . (broadinstitute.org)
These data, along with statistical models, allow us to see otherwise-hidden connections between genetic and epigenetic errors in cancer cells and changes in those cells' metabolic profiles," Li said. (broadinstitute.org)
The cytotoxicity and anti-tumour effects of strawberry tree honey in relation to colon cancer lines increased with the amount and time of treatment. (agenciasinc.es)
MD Anderson Cancer Center is in line to receive $100 million in publicly traded stock as part of a licensing agreement that involves one of the most exciting emergent treatment avenues in oncology. (lymphomainfo.net)
it involves customizing T cells to find and kill cancer cells. (lymphomainfo.net)
Genetically engineering our patients' immune-system T cells to efficiently attack and destroy cancer cells represents one of the most exciting approaches with curative potential in oncology today," said MD Anderson President Dr. Ron DePinho. (lymphomainfo.net)
We believe coupling MD Anderson's unique CAR T cell approach with the powerful technologies of ZIOPHARM and Intrexon will allow us to build T cells that hit cancer harder, with greater precision, under tighter control and with potentially fewer side effects for patients. (lymphomainfo.net)
Merkel Cell Carcinoma (MCC) is a very rare and aggressive skin cancer that usually develops when a person is in his or her 70s. (lymphomainfo.net)
Surgery is recommended for patients with stage I or II non-small cell lung cancer NSCLC) and may provide the best possibility for a cure. (medscape.com)
Some people worry that cell (wireless) phones cause cancer or other health problems. (medlineplus.gov)
arations verified the presence of cancer cells in all samples. (lu.se)

Add 2 ml of the Jurkat cell suspension to a new sterile T75 flask containing 20 ml of complete growth medium and a final concentration of 1 mg/ml G418. (neb.com)
This study examined the post-thaw recovery of Jurkat cells cryopreserved in single osmolyte solutions containing sucrose, glycerol or isoleucine, as well as in a combination of the three osmolytes. (nature.com)
Post-thaw recovery of Jurkat cells in single osmolyte was low. (nature.com)
These studies demonstrated that a combination of three different osmolytes including sugar, sugar alcohol and amino acids/proteins could stabilize Jurkat cells and mesenchymal stromal cells (MSCs) during freezing. (nature.com)
The objective of this investigation is to understand in more detail the relationships amongst the osmolytes present in these solutions and Jurkat cell recovery. (nature.com)

In addition, further engineered cell lines can be offered, as reporter cell lines, or overexpression stable cell lines. (biocat.com)

Selpercatinib should be considered a first-line standard of care in RET fusion-positive advanced NSCLC," the authors write. (medscape.com)
Cite this: Selpercatinib Extends PFS in First-line RET-Positive NSCLC - Medscape - Nov 06, 2023. (medscape.com)

Results and Conclusions: The adaptation process was characterized by initial cell population growth arrest, and after that extensive cell death, followed by proliferation and long-term survival of clonal cultures. (lu.se)

Add 10 ml of fresh complete growth medium and gently resuspend cell pellet by pipetting up and down 2-3 times. (neb.com)
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. (abcam.com)

It has also been used to study placental transfer of highly purified non-dioxin-like polychlorinated biphenyls in BeWo cells. (sigmaaldrich.com)

These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus. (thermofisher.com)

Dimethyl sulfoxide (DMSO) has been the standard cryopreservation agent for freezing cells since the 1960 s 2 . (nature.com)

The single largest bank of isogenic cell lines with over 7,500 cell lines to choose from and trusted by academia, biotech, and pharma research labs. (horizondiscovery.com)
At some point, the Seattle biotech company Cell Therapeutics Inc (CTI) should earn an entry in the Guinness Book of World Records for utter and. (lymphomainfo.net)

Services Laboratories, Ames, IA, USA), which was initially in the United States: clinical signs, lesions, and viral genomic passaged 5 times in Vero cells, was used to infect the MK- sequences. (cdc.gov)
It's a call that's finally, increasingly being communicated: to analyze the cells you're going to use by genomic analysis. (the-scientist.com)

Within this study line, you will obtain competences in designing, constructing, and optimizing cell factories using either bacterial, yeast, molds, plant or animal cell cultures. (dtu.dk)
Proliferation and cell cycle progression of the serum-free cultures closely mimicked that of serum-dependent cells. (lu.se)

Near confluent cells were infected with been isolated in the United States, making development of a PEDV at a multiplicity of infection of 0.1. (cdc.gov)
B) PEDV-induced cytopathic effect in MK-DIEC cells 36 h after infection. (cdc.gov)

This practice adds to the complexity of the workflow associated with preservation of cells using DMSO. (nature.com)
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose. (abcam.com)
1 x 10E7 cells are supplied frozen in 1 ml of 90% complete medium and 10% DMSO. (thermofisher.com)

T-Mobile in February announced it would embark on a "significant" program to deploy 25,000 small cells in 2018 and early 2019. (fiercewireless.com)

Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x10 4 cells/cm 2 . (abcam.com)

Remove and discard 10 ml of old growth medium from above the cell pellet. (neb.com)

Generally the default display in BioGPS expression viewer/activity chart is an average of the two cell lines. (google.com)
Most importantly, GFP expression recapitulated endogenous Olig2 expression when R-Olig2 was induced by sonic hedgehog and retinoic acid, and GFP-positive cells could be purified by fluorescence-activated cell sorting. (ca.gov)
Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)

The HEL cell line is an immortalised cell line from a 30-year old male Acute erythroid leukemia patient, used in biomedical research. (wikipedia.org)
According to study coauthor and Cambridge Biomedical Research Campus medical geneticist Serena Nik-Zainal , the study reveals that the level of quality control involved in such stem cell research may not be up to snuff. (the-scientist.com)
It's a problem, but it's solvable," says Jeanne Loring , a stem cell researcher at the Scripps Research Institute who didn't work on the study. (the-scientist.com)
As it stands now, scientists using these hiPSCs for research or clinical applications only need to screen and characterize their cell lines, whether they're developed in-house or taken from a repository, to whatever extent is required by a journal or the reviewers assessing their work. (the-scientist.com)
This cell line may be licensed nonexclusively for research or commercial purposes. (mskcc.org)
Can't find the right cell model for your research? (horizondiscovery.com)
The research was funded by the National Institutes of Health and the Johns Hopkins Institute for Cell Engineering. (hopkinsmedicine.org)

Sharma, S. V., Haber, D. A. & Settleman, J. Cell line-based platforms to evaluate the therapeutic efficacy of candidate anticancer agents. (nature.com)

This is on top of the approximately 18,000 small cells and DAS nodes already rolled out as of the end of 2017," the company said at the time. (fiercewireless.com)
A recent report from industry trade group CTIA said that the number of small cells in the United States will grow from 13,000 in 2017 to 86,000 this year-a 550% increase-and to more than 800,000 by 2026. (fiercewireless.com)

Pluristem Therapeutics , a leading developer of placenta-based cell therapy products, has announced that it has completed development of its second major product line, called PLX-RAD. (israel21c.org)
We believe that our state-of-the-art technology platform can be used to create additional cell products from the placenta, tailored to potentially deliver targeted treatments for a variety of new indications," stated Zami Aberman, Chairman and CEO of Pluristem. (israel21c.org)

Predicting drug susceptibility of non-small cell lung cancers based on genetic lesions. (nature.com)
Those 'normal' cell lines have as many mutations as [some] cancers," Nik-Zainal tells The Scientist . (the-scientist.com)

Anatomy7

Organisms1

Chemicals and Drugs2

Analytical, Diagnostic and Therapeutic Techniques and Equipment4

Phenomena and Processes14

Information Science3

Hematopoietic stem1

Embryonic12

Vitro8

Knockout4

Tumor3

CRISPR1

Metastatic5

Human22

Gene6

Vivo4

Antibodies2

Somatic cells1

Stem cells9

Differentiation1

Assay2

20201

Sensitivity in cancer3

Distinct cancer subtypes2

Toxicity4

Therapies2

Subculture1

Genes7

Apoptosis1

Viability1

Genome1

Protein3

Researchers4

Genetically engineered2

Tissues4

Tumors1

Receptor2

Cancer15

Jurkat5

Stable cell1

NSCLC2

Clonal1

Resuspend2

Highly purified1

Stably1

Cryopreservation1

Biotech2

Genomic2

Cultures2

Infection2

DMSO3

20191

Flask1

Pellet1

Expression3

Research7

Therapeutic1

20172

Placenta2

Cancers2