AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesDisciplines and OccupationsInformation ScienceNamed GroupsHealth Care
KaryotypingSpectral KaryotypingChromosome AberrationsCell LineKaryotypeChromosome DisordersIn Situ Hybridization, FluorescenceTrisomyPrenatal DiagnosisAmniocentesisCell Line, TumorAneuploidyChromosome BandingCytogeneticsFetal DiseasesDown SyndromeCytogenetic AnalysisChorionic Villi SamplingSex Chromosome DisordersChromosome PaintingUltrasonography, PrenatalChromosomes, HumanTranslocation, GeneticPregnancyComparative Genomic HybridizationAbnormal KaryotypeChromosomes, Human, Pair 18Ring ChromosomesChromosomes, Human, Pair 13NeckSex Chromosome AberrationsAbnormalities, MultipleAzure StainsChromosome DeletionCell Line, TransformedChromosomes, Human, Pair 20Pregnancy Trimester, FirstNectriaChromosomes, Human, Pair 7ChromosomesMyelodysplastic-Myeloproliferative DiseasesPregnancy, High-RiskMetaphaseNucleic Acid HybridizationCrown-Rump LengthUniparental DisomyMaternal AgeNuchal Translucency MeasurementMycological Typing TechniquesPregnancy Trimester, SecondAbortion, HabitualMosaicismIntellectual DisabilityOligonucleotide Array Sequence AnalysisNeoplasms, Adipose TissueGenetic TestingAzoospermiaGene RearrangementChromosomes, Human, YChromosomes, Human, Pair 5Sex Chromosome Disorders of Sex DevelopmentChromosomes, Human, Pair 14Chromosomes, Human, XPregnancy OutcomeChromosomes, Human, Pair 21Polymerase Chain ReactionKlinefelter SyndromePhenotypeGestational AgeMonosomyDNA, FungalChromosome BreakageChromosomes, Artificial, BacterialGene DosageAbortion, SpontaneousMyelodysplastic SyndromesChromosomes, Human, Pair 8Cytoplasmic DyneinsTurner SyndromeTumor Cells, CulturedSex Determination AnalysisChromosome MappingBase SequenceChromosomal InstabilitySyndromeMolecular Sequence DataPulmonary AtresiaGene AmplificationFlow CytometryDNA Copy Number VariationsPreimplantation DiagnosisAmniotic FluidChromosomes, Human, Pair 22Genome, HumanFetusInfertilityMutationInfant, NewbornChromosomes, Human, Pair 12Chromosomes, Human, Pair 11
ChromosomesMorphologySpectralChromosome rearrangementsDiploidGeneticDifferentiationEmbryonic StemVitroCharacterizationGeneChromosomal abnormalitiesMicroarrayModalLaboratoryCytogeneticsNormalTumorMycoplasmaGenomicAnalysisAnalysesDifferentialMolecularAbnormalCell LinesParentalVariationTypicallyHESC linesDifferentiateCellsGerm lineHumanClonesTurnerSpeciesFluorescenceRepositoryOrganoidsSuccessfullyEvolutionPassagesPassageStudyCultureConsistentProbeIdentitySkin
Chromosomes10
Morphology5
  • Three from 780757-88-2 the produced immortalized fibroblast lines 780757-88-2 had been characterized regarding to morphology, proliferation dynamics, and gene appearance. (biopaqc.com)
  • 2007) (Figures S1H, S2A and Supplemental Experimental Procedure), hiPSC lines were differentiated into hematopoietic progenitor lineages, showing common morphology for the various cell types (Physique S1H). (eprf.ca)
  • Targeted clone R-Olig2 (like the other clones) retained pluripotency, typical hESC morphology, and a normal parental karyotype 46,XY. (ca.gov)
  • The IPSCs display pluripotent cell morphology, expression of pluripotency markers, normal karyotype, and the capability to differentiate into all three germ layers. (curefa.org)
  • cell authentication (STR), pluripotency and undifferentiated state confirmation, molecular karyotype, g-banding and iPS growth pattern and morphology analysis. (lu.se)
Spectral1
  • Spectral karyotyping analysis (also called chromosome painting) uses chromosome-specific multicolor fluorescent in situ hybridization (FISH) techniques that improve the visibility of certain defects, including translocations and inversions. (msdmanuals.com)
Chromosome rearrangements1
  • Cytogenetics work has established the normal Tasmanian Devil karyotype and the chromosome rearrangements of the tumour. (agriculture.gov.au)
Diploid2
  • There are virtually no diploid cell lines used in bioprocessing today," Dr. Wurm continued, "and it's a good thing we didn't follow recommendations to use them that were current 20 years ago, as it would have set back the industry tremendously. (genengnews.com)
  • Karyotype analyses indicate that the ReNcell CX retains a normal diploid karyotype in culture even after prolonged passage (>45 passages). (thomassci.com)
Genetic10
  • MedlinePlus offers additional details about karyotype genetic tests . (medlineplus.gov)
  • The University of Utah's Genetic Science Learning Center discusses how karyotypes can be used in diagnosing genetic disorders . (medlineplus.gov)
  • A total of 7 possible genetic alterations and epigenetic changes in CAFs are discussed, including gene differential expression, karyotype analysis, gene copy number variation, loss of heterozygosis, allelic imbalance, microsatellite instability, post‑transcriptional control and DNA methylation. (spandidos-publications.com)
  • Nei's genetic distances suggest that T. occidentalis , T. romana and T. stankovici early diverged from an europaea - caeca line. (bioone.org)
  • A genetic test called a karyotype analysis can confirm a Turner syndrome diagnosis. (winetourismday.org)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • A karyotype test is used to help diagnose genetic diseases, certain birth defects, as well as particular hematologic and lymphoid abnormalities. (howmuchisit.org)
  • It is recommended that you have genetic counseling prior to deciding about a karyotype test. (howmuchisit.org)
  • Genotype: The genetic constitution determined by the karyotype. (msdmanuals.com)
  • As with other genetic abnormalities, definite diagnosis is only confirmed via karyotyping 1-3 . (bvsalud.org)
Differentiation2
  • This report demonstrates that Synthemax II-SC supports long-term culture of H9 and H14 hESC lines and permits efficient differentiation of hESCs into functional RPE. (ca.gov)
  • The permanent cell line (BXD-1425EPN), which was derived from a passage II xenograft tumor and has been passaged in vitro more than 70 times, expressed similar differentiation markers of the xenograft tumors, maintained identical chromosomal abnormalities, and formed tumors in the brains of SCID mice. (nih.gov)
Embryonic Stem1
  • A targeted neuroglial reporter line generated by homologous recombination in human embryonic stem cells. (ca.gov)
Vitro5
  • Recently, several studies have analysed the genomic stability of several hESC lines maintained after prolonged in vitro culture but controversial data has been reported. (nih.gov)
  • Single-cell karyotype sequencing confirmed heterogeneity of copy number alterations in tumor PDOs and showed that monoclonal lines evolved novel karyotypes over time in vitro. (nature.com)
  • Fig. 6: In vitro karyotype evolution of tumor PDO 16T. (nature.com)
  • Test chemical did not induce mutation in mammalian cell line in the presence and absence of metabolic activation and hence it is not likely to classify as a gene mutant in vitro. (europa.eu)
  • CHO cells have a highly variable and unstable karyotype, given that they are permanently transformed cells that have been cultured in vitro for decades. (genengnews.com)
Characterization2
  • As a recognized world leader in pluripotent stem cell banking and characterization, WiCell provides the stem cell community with high-quality cell lines as well as accurate and reliable characterization testing. (wicell.org)
  • basic characterization, including cell authentication (STR), undifferentiated state (FACS) and molecular karyotyping. (lu.se)
Gene8
  • Human BeWo cell line has been used to study the effects of Δ-9-tetrahydrocannabinol (Δ9-THC) on the proliferation and gene transcription in the BeWo trophoblast cell line. (sigmaaldrich.com)
  • The resulting insertional mutation creates a fusion transcript containing sequences from exons upstream of the insertion joined to the β-geo marker, allowing cell lines where the vector has successfully interrupted a gene to be identified. (mmrrc.org)
  • The vast majority of gene trap cell lines have been correctly identified, as judged by DNA sequencing (i.e., repeat determination of the DNA sequence tag when the ES cell clone is requested). (mmrrc.org)
  • If more than one ES cell line is available for your "gene-of-interest", we recommend that you order several of the ES cell lines. (mmrrc.org)
  • Ordering more than one ES cell line for a specific 'gene-of-interest' will increase the chances of success. (mmrrc.org)
  • MMRRC Testing of the BayGenomics, SIGTR, and Soriano gene trap cell lines includes RACE sequencing and mycoplasma and MPV testing. (mmrrc.org)
  • The present work reports an hESC reporter line generated by homologous recombination targeting a neural lineage-specific gene, which can be differentiated and sorted to obtain pure neural progenitor populations. (ca.gov)
  • This paper presents the generation of an induced pluripotent stem cell (iPSC) line from an FA patient with a homozygous GAA expansion in intron 1 of the FXN gene. (curefa.org)
Chromosomal abnormalities1
  • Karyotypes have been used for several decades to identify chromosomal abnormalities in both germline and cancer cells. (wikipedia.org)
Microarray1
  • Other terms used to describe the arrays used for karyotyping include SOMA (SNP oligonucleotide microarrays) and CMA (chromosome microarray). (wikipedia.org)
Modal1
Laboratory4
  • Array-based karyotyping can be done with several different platforms, both laboratory-developed and commercial. (wikipedia.org)
  • Here, we prompted to compare the chromosomal stability of three hESC lines maintained in the same laboratory using identical culture conditions and passaging methods. (nih.gov)
  • These divergences confirm that these isolates have undergone substantial development in the laboratory since the Bge karyotype was first assessed and retained the expected 2= 36 diploidy (Bayne et al. (mingsheng88.org)
  • According to HealthTap.com , a karyotype test can cost about $2,000 depending on which laboratory it is sent to. (howmuchisit.org)
Cytogenetics2
Normal3
  • Both hESCs and hESC-RPE maintained normal karyotypes after long-term culture on Synthemax II-SC. (ca.gov)
  • All hiPSC lines had a AM 114 normal karyotype (Physique S1C). (eprf.ca)
  • Regardless of whether the karyotype test is normal or abnormal, you will usually need a follow-up appointment to discuss the results with your doctor. (howmuchisit.org)
Tumor2
  • Fig. 4: Single-cell genome sequencing reveals karyotype heterogeneity in tumor PDOs. (nature.com)
  • In conclusion, direct injection of primary ependymoma tumor cells played an important role in the generation of a clinically relevant mouse model IC-1425EPN and a novel cell line, BXD-1425EPN. (nih.gov)
Mycoplasma2
  • Requested cell lines are tested by PCR analysis for mycoplasma spp . (mmrrc.org)
  • Deliver 2 clonally expanded mycoplasma free iPSC lines (15 x 300k cells). (lu.se)
Genomic5
Analysis4
  • Virtual karyotype is the digital information reflecting a karyotype, resulting from the analysis of short sequences of DNA from specific loci all over the genome, which are isolated and enumerated. (wikipedia.org)
  • We also recommend that recipients of ES cells re-verify the identity of the ES cell line by RT-PCR and/or Southern blot analysis. (mmrrc.org)
  • Flow cytometry analysis showed that this differentiating lines expressed the pan-hematopoietic marker CD45 at least until Day 28 (Physique S2B). (eprf.ca)
  • Karyotype analysis was performed by the G-banding technique. (hindawi.com)
Analyses1
  • Molecular cytogenetic analyses performed in three different hESC lines maintained in parallel in identical culture conditions revealed significant differences among them in regard to their chromosomal integrity. (nih.gov)
Differential1
  • Human ESCs predisposition to karyotypic instability: Is a matter of culture adaptation or differential vulnerability among hESC lines due to inherent properties? (nih.gov)
Molecular1
Abnormal1
  • If the results were abnormal, the doctor may also order other examinations that go together with the karyotype test, or the patient may need to start treatment for a certain condition. (howmuchisit.org)
Cell Lines9
  • One-way ANOVA was performed to compare the %GFP+ cells and viability of organoids from four different cell lines. (elifesciences.org)
  • Karyotypes of induced pluripotent stem cell lines. (elifesciences.org)
  • Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. (mmrrc.org)
  • All mutant ES cell lines are heterozygous for the mutation, meaning they carry one modified allele and one wild type allele. (mmrrc.org)
  • WiCell has characterized cell lines for Fate, and they have delivered high quality study results as well as outstanding and knowledgeable service and technical support. (wicell.org)
  • The conventional rationale for obtaining high producers is based on examination of transfected cell lines by low-throughput screening to identify the top producing clones," Dr. Wurm continued. (genengnews.com)
  • Biology plays a dominant role in cell performance," Dr. Wurm added, believing that the karyotypic makeup of the transfected cell lines needs to be carefully monitored during expansion. (genengnews.com)
  • ReNcell NSC Freezing Medium (SCM007): ReNcell NSC Freezing Medium is qualified for use with ReNcell immortalized human neural progenitor cell lines, CX (CHEMICON Catalog No. SCC007) and VM (CHEMICON Catalog No. SCC008) cultured in serum-free conditions with ReNcell NSC Maintenance Medium (CHEMICON Catalog No. SCM005). (thomassci.com)
  • The presence of ≥ 2 cell lines differing in genotype in a person who has developed from a single fertilized egg. (msdmanuals.com)
Parental1
  • The parental line, AG07680, from a 4 to 5 month old fetus, was initiated on 4/25/79 using cells released by collagenase digestion of the lumen of the thoracic aorta. (coriell.org)
Variation1
  • The study revealed that karyotype variation reflected in differences in the participants' cognitive profile. (bvsalud.org)
Typically1
  • Karyotype testing is typically covered by most insurance companies as long as it was ordered by a licensed physician and is medically necessary. (howmuchisit.org)
HESC lines4
  • In feeders, the HS181, SHEF-1 and SHEF-3 hESC lines were chromosomally stable up to 185 passages using either mechanical or enzymatic dissection methods. (nih.gov)
  • Despite the three hESC lines were maintained under identical conditions, each hESC line behaved differently upon being transferred to a feeder-free culture system. (nih.gov)
  • The two younger hESC lines, HS181 (71 passages) and SHEF-3 (51 passages) became chromosomally unstable shortly after being cultured in feeder-free conditions. (nih.gov)
  • These results support the concept that feeder-free conditions may partially contribute to hESC chromosomal changes but also confirm the hypothesis that regardless of the culture conditions, culture duration or splitting methods, some hESC lines are inherently more prone than others to karyotypic instability. (nih.gov)
Differentiate1
  • ReNcell CX Immortalized Cells (SCC007): ReNcell CX is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. (thomassci.com)
Cells5
  • Historically, karyotypes have been obtained by staining cells after they have been chemically arrested during cell division. (wikipedia.org)
  • By periodically monitoring the karyotype of the cells during the scale-up process, it is possible to guarantee the presence of the target marker, assuring that the cell will in fact produce the desired protein. (genengnews.com)
  • Results Generation of hiPSC lines and clinical manifestations in NS/JMML-derived hematopoietic cells Using somatic cell reprogramming (Takahashi et al. (eprf.ca)
  • ReNcell CX Human Neural Progenitor Cell Line & Culture Media Kit ReNcell CX Kit contains human neural progenitor cells, optimized maintenance media & freezing media for culture of human neural stem cells. (thomassci.com)
  • Malignancies involving mesothelial cells that normally line the body cavities, including the pleura (see the image below), peritoneum, pericardium, and testis, are known as malignant mesothelioma. (medscape.com)
Germ line1
  • For NS, particular germ-line mutations in underlie JMML, which has clinical features similar to those in children with JMML arising from somatic mutations in although with generally better outcomes. (eprf.ca)
Human4
  • This is a hypotriploid human cell line. (atcc.org)
  • However, incorrectly identified ES cell clones are occasionally encountered, either because of human error or because a thawed ES cell line gets overgrown by a contaminating ES cell line. (mmrrc.org)
  • Using FISH, Dr. Wurm was able to show the regions that carry the human DNA within the hamster karyotype. (genengnews.com)
  • In this study, we targeted Olig2, a basic helix-loop-helix transcription factor that plays an important role in motoneuron and oligodendrocyte development, in human embryonic stem cell (hESC) line BG01 by homologous recombination. (ca.gov)
Clones1
  • MMRRC also recommends performing chromosome counts or karyotyping prior to injection of clones. (mmrrc.org)
Turner2
Species3
  • We argue that the observed process of chromosome sorting would, if continued, result in a new homozygous chromosomal race, i.e., in a new karyotype with intermediate chromosome number and, possibly, in a new incipient homoploid hybrid species. (frontiersin.org)
  • A karyotype (Fig 1) is the characteristic chromosome complement of a eukaryote species. (wikipedia.org)
  • The karyotypes of moles are very conservative, with the majority of the species showing 2n = 34. (bioone.org)
Fluorescence1
  • The pink dash line shows the mean fluorescence intensity of DMSO-treated organoids. (elifesciences.org)
Repository1
  • Estimated timelines: The repository was sent only one or two vials per clone/cell line from the production center. (mmrrc.org)
Organoids1
  • We conclude that ongoing CIN is common in colorectal cancer organoids, and propose that CIN levels and the tolerance for mitotic errors shape aneuploidy landscapes and karyotype heterogeneity. (nature.com)
Successfully1
  • Sperm from patients with the nonmosaic 47,XXY karyotype has been used successfully in assisted reproduction. (medscape.com)
Evolution1
  • We also discuss the segregational model of karyotype evolution and the chromosomal model of homoploid hybrid speciation. (frontiersin.org)
Passages1
  • Strikingly, however, a much older hESC line, SHEF-1, which was maintained for 185 passages in feeders did not undergo any numerical or structural chromosomal change after 30 passages in feeder-free culture and over 215 passages in total. (nih.gov)
Passage2
  • The HS181 line gained a chromosome 12 by passage 17 and a marker by passage 21, characterized as a gain of chromosome 20 by SKY. (nih.gov)
  • Similarly, the SHEF-3 line also acquired a trisomy of chromosome 14 as early as passage 10. (nih.gov)
Study1
  • The test chemical did not induce chromosome aberrations in the mammalian cell line in the presence and absence of S9 metabolic activation system and hence it is not mutagenic in the chromosome aberration study performed. (europa.eu)
Culture1
  • Culture, line BFAE-39e, medium: HF12 15I. (coriell.org)
Consistent1
  • After EGF stimulation, ERK activation was increased and sustained in the NS and NS/JMML hiPSC lines (Physique S1G), consistent with mutation GOF effects. (eprf.ca)
Probe1
  • citation needed] Knowing the address of each probe on the array and the address of each probe in the genome, the software lines up the probes in chromosomal order and reconstructs the genome in silico (Fig 2 and 3). (wikipedia.org)
Identity1
  • Therefore, we strongly recommend that you request verification of the identity of your ES cell line by DNA sequencing (there is an additional fee for DNA sequence verification of your clone). (mmrrc.org)
Skin2
  • 2007), we obtained hiPSC lines from skin fibroblasts harboring mutations in from two subjects with NS/JMML. (eprf.ca)
  • As controls, we used five AM 114 hiPSC lines derived from skin fibroblasts of unrelated healthy individuals and two subjects with NS, the latter to clarify which perturbations were attributable to the gain-of-function mutations generally those specific to JMML pathogenesis (Tables S1 and S2). (eprf.ca)