AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation Science
Hydroxymethylglutaryl-CoA-Reductases, NADP-dependentCarcinoma, HepatocellularLiver NeoplasmsTumor Cells, CulturedHep G2 CellsCell LineCell Line, TumorHempaCell Line, TransformedRNA, MessengerPropyl GallateEmeticsPlasminogen InactivatorsLiverTransfectionMolecular Sequence DataBase SequenceLovastatinApolipoprotein A-ILiver Neoplasms, ExperimentalKineticsArtificial OrgansHepatoblastomaAsialoglycoprotein ReceptorReceptors, LDLCarboxypeptidase HTranscription, GeneticOrganosilicon CompoundsOxaloacetic AcidPromoter Regions, GeneticMoraceaeLipoproteins, LDLBlotting, NorthernGene Expression RegulationApoptosisOleic AcidApolipoproteins ACell SurvivalBlotting, WesternCholesterolApolipoproteins BHydroxycholesterolsHydroxymethylglutaryl CoA ReductasesMevalonic AcidImmunosorbent TechniquesCycloheximideDNACells, CulturedGene ExpressionAmino Acid SequencePrimaquineLipoproteins, HDLPhenylenediaminesAsialoglycoproteinsOleic AcidsDose-Response Relationship, DrugMolecular WeightGlycoproteinsBiological TransportImmunoelectrophoresis, Two-DimensionalTunicamycinCloning, MolecularGene Expression Regulation, NeoplasticElectrophoresis, Polyacrylamide GelRecombinant ProteinsRNA, NeoplasmAntineoplastic Agents, PhytogenicCarrier ProteinsCell ProliferationApolipoprotein A-IIAlbuminsDNA-Binding ProteinsAntineoplastic AgentsTranscription FactorsPolymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionChenodeoxycholic AcidTime FactorsPlasmidsMice, NudeVitamin KMutationReceptors, Cell SurfaceCytochrome P-450 CYP2E1PhenotypeApolipoprotein B-100Signal Transduction
Hepatocellular carcinomaVirologyMCF7LiverDifferentiationLipidSuitableEpithelialKnockout cellCytotoxicityMorphologicalImmortalStimulationRobustSignificantlyApoptosisKidneyIntracellularStudyAssayVitroMammalianAdherentVirusTissueMonolayerCultureConditionsShownFoundResultsNormalVirusesJournalDrugTypeProteinGrowthPropertiesMediumMajorDistribution
Hepatocellular carcinoma3
  • The Hep G2 cell line has been isolated from a liver biopsy of a male Caucasian aged 15 years, with a well differentiated hepatocellular carcinoma. (sigmaaldrich.com)
  • Hep G2 is an immortal cell line which was derived in 1975 from the liver tissue of a 15-year-old Caucasian male from Argentina with a well-differentiated hepatocellular carcinoma. (wikipedia.org)
  • Chronic hepatitis B virus (HBV) and hepatitis D virus (HDV) infections are major causes of liver disease and hepatocellular carcinoma worldwide. (mdpi.com)
Virology1
  • Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
MCF72
  • MTT assay was employed to study the anticancer activity against cancer cell lines Hep-G2, MCF7 and normal VERO cell lines. (google.com)
  • Crude methanolic extract of G. corticata had significant anticancer activity followed by E. antenna and E. linza on cancer cell lines Hep-G2, MCF7 and normal VERO cell lines by MTT assay. (google.com)
Liver6
  • Hep G2 (or HepG2) is a human liver cancer cell line. (wikipedia.org)
  • This can be important for the study of human liver diseases that are caused by an incorrect subcellular distribution of cell surface proteins, e.g., hepatocanalicular transport defects such as Dubin-Johnson Syndrome and progressive familial intrahepatic cholestasis (PFIC), and familial hypercholesterolemia. (wikipedia.org)
  • citation needed] Hep G2 cells and their derivatives are also used as a model system for studies of liver metabolism and toxicity of xenobiotics, the detection of environmental and dietary cytotoxic and genotoxic (and thus cytoprotective, anti-genotoxic, and cogenotoxic) agents, understanding hepatocarcinogenesis[citation needed], and for drug targeting studies[citation needed]. (wikipedia.org)
  • Hep G2 cells are also employed in trials with bio-artificial liver devices[citation needed]. (wikipedia.org)
  • The current study found that ST has the capacity to induce cell toxicity in human liver cells. (phcogj.com)
  • A human liver tumor cell line used to study a variety of liver-specific metabolic functions. (bvsalud.org)
Differentiation3
  • Another well-characterized polarized hepatocyte cell line is the rat hepatoma-derived hybrid cell line WIF-B. With the proper culture conditions, Hep G2 cells display robust morphological and functional differentiation with a controllable formation of apical and basolateral cell surface domains (van IJzendoorn et al. (wikipedia.org)
  • Because of their high degree of morphological and functional differentiation in vitro, Hep G2 cells are a suitable model to study the intracellular trafficking and dynamics of bile canalicular, sinusoidal membrane proteins, and lipids in human hepatocytes in vitro. (wikipedia.org)
  • Therefore, phenotypic variability might differ depending on the polarization/differentiation of the cells. (bvsalud.org)
Lipid1
  • The aim of this study was to analyze the insulin-mediated response of PAI-1 and lipid synthesis in Hep G2 cells after producing an insulin-resistant state by decreasing insulin receptor numbers. (jci.org)
Suitable2
  • Hep G2 cells may be a suitable model to study PAI-1 regulation in response to hyperinsulinemia. (jci.org)
  • citation needed] Hep G2 cells are a suitable in vitro model system for the study of polarized human hepatocytes. (wikipedia.org)
Epithelial2
  • These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice. (wikipedia.org)
  • Wilson's disease (WD) primarily affects organs composed of polarized/differentiated epithelial cells. (bvsalud.org)
Knockout cell4
  • Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1. (abcam.com)
  • Knockout cell lysate achieved by CRISPR/Cas9. (abcam.com)
  • Access thousands of knockout cell lysates, generated from commonly used cancer cell lines. (abcam.com)
  • See here for more information on knockout cell lysates. (abcam.com)
Cytotoxicity3
  • Hep G2 cell line human has been used to study the cytotoxicity of carbon monoxide-releasing molecules and organic solvents on HepG2 cells and to evaluate the antiproliferative activity of aqueous extracellular polysaccharides (AEPS) for HepG2 using the MTT colorimetric assay. (sigmaaldrich.com)
  • Four concentrations (10, 25, 50, and 100 μg/mL) were selected for the genotoxic assessment for PBMC and three (1.25, 2.5, and 5 μg/mL) for HepG2 cells based on cytotoxicity tests (MTT assay). (geneticsmr.org)
  • When in vitro experimental models are exposed to ATZ, they are faced with increased levels of reactive oxygen species (ROS), cytotoxicity and decreased growth rate at dosages that may vary with cell types. (frontiersin.org)
Morphological1
  • ATZ also induces morphological changes similar to apoptosis and initiates mitochondria-dependent cell death in several experimental models. (frontiersin.org)
Immortal1
  • The lifespan of most cells is genetically determined, but some cell-culturing cells have been "transformed" into immortal cells which will reproduce indefinitely if the optimal conditions are provided. (wikipedia.org)
Stimulation2
  • The cells will respond to stimulation with human growth hormone. (sigmaaldrich.com)
  • Preincubation by an 18-h exposure of Hep G2 cells to 10(-7) M insulin aimed at reducing the number of insulin receptors, was followed by a subsequent 24-h stimulation with 10(-9) M insulin. (jci.org)
Robust2
  • While the molecular biology of the two viruses has been characterized in great detail, the absence of robust cell culture models for HBV and/or HDV infection has limited the investigation of virus-host interactions. (mdpi.com)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
Significantly4
  • An early apoptosis induction in HepG2 cells was observed by annexin V assay, which clearly exhibited significantly increased early and late apoptosis phases both at 24 and 48 h. (phcogj.com)
  • A significantly increasing pattern of hypodiploid phases of cells also been observed, which confirm the apoptosis induction again. (phcogj.com)
  • The transcription of B7-H1 mRNA was increased significantly in these cells compared to cells transfected with control vector (HBx(-)-HepG2), as confirmed by a comparative genome-wide microarray analysis (Capitalbio) and real time quantitative PCR (qPCR). (nih.gov)
  • Manipulating lysosomal positioning significantly alters the localisation of ATP7B in the cell. (bvsalud.org)
Apoptosis2
  • Co-culture experiments with HBx(+)-HepG2/T cells showed that the number of apoptotic T cells increased profoundly, and this effect could be partially prevented when a neutralizing mAb against B7-H1 was added to the culture, demonstrating that B7-H1 signaling can promote T cell apoptosis. (nih.gov)
  • Our results suggest that the expression of B7-H1 in hepatocarcimona cells can be initiated by HBx antigen, thus inducing T cell apoptosis and finally potentially facilitates the genesis of HCC. (nih.gov)
Kidney1
  • This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
Intracellular1
  • The present study investigates the intracellular stability and localization of ATP7B harboring WD mutations in both unpolarized/undifferentiated and polarized/differentiated cell-based models. (bvsalud.org)
Study4
  • These cell lines were tested as part of another study ( 14 ), and the results confirmed as part of this study. (cdc.gov)
  • Collectively, results presented in this study demonstrated that the ST, which is used as a euphoritic substance of abuse also, has significant level of toxicity in human cells. (phcogj.com)
  • In view of these considerations, this study was designed to evaluate the cytotoxic and genotoxic potential of geraniol, in an in vitro protocol, using two types of human cells: one without the ability to metabolize (peripheral blood mononuclear cells - PBMC), and the other with this capability (human hepatoma cell line - HepG2) through the comet assay and the micronucleus test. (geneticsmr.org)
  • While mutations in the proximal C-terminus affect ATP7B stability, the present study shows that the distal region dictates cell-specific Trans Golgi Network (TGN) localization and exit. (bvsalud.org)
Assay1
  • This results in differing cytotoxic responses that are influenced by the nature of target cells, assay types and concentrations of ATZ. (frontiersin.org)
Vitro1
  • This review focuses on the differential responses of cell types to ATZ toxicity, testicular effects of ATZ in both in vitro and in vivo models and chemopreventive strategies, so as to highlight the current state of the art on the toxicological outcomes of ATZ exposure in several experimental model systems. (frontiersin.org)
Mammalian1
  • Atrazine (ATZ) is an environmental pollutant that interferes with several aspects of mammalian cellular processes including germ cell development, immunological, reproductive and neurological functions. (frontiersin.org)
Adherent1
  • Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)
Virus2
  • Hepatitis B virus surface antigens have not been detected. (sigmaaldrich.com)
  • The cross-talk between the hepatitis B virus X protein (HBx) and B7-H1 in hepatocarcinoma (HCC) is unclear. (nih.gov)
Tissue7
  • Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
  • After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
  • [12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
Monolayer1
  • 37°C. Cells might take 6 days to form a confluent monolayer without media change if seeded at 1:4. (sigmaaldrich.com)
Culture6
  • 2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. (abcam.com)
  • 3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. (abcam.com)
  • Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x10 4 cells/cm 2 . (abcam.com)
  • Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
  • The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)
  • Although curcumin has shown significant efficacy in cell culture studies, it has shown limited efficacy in clinical studies when administered in conventional oral formulations. (researchgate.net)
Conditions1
  • Results showed that geraniol did not present genotoxic or clastogenic/aneugenic effects on both cell types under the conditions studied. (geneticsmr.org)
Shown2
  • The hepatoma cell line Hep G2 has been shown to synthesize PAI-1 in response to insulin. (jci.org)
  • Hep G2 cells have been shown to be G418 resistant (400 g/ml). (hsanmartino.it)
Found3
  • The inhibitory capacity of ST in HepG2 and WRL 68 has been found to be 151 ± 2.5 and 305 ± 11.5 μg/ml for 24 h. (phcogj.com)
  • If you require a native form of the protein please use the live cell version - found here . (abcam.com)
  • We found that in basal copper or even upon copper chelation, a significant amount of ATP7B persists in the endolysosomal compartment of hepatocytes but not in non-hepatic cells. (bvsalud.org)
Results1
  • The results indicate that PAI-1 synthesis in presence of insulin is markedly increased in down-regulated cells, and that metformin inhibits this effect by acting at the cellular level. (jci.org)
Normal1
  • However, caution is advised in the use of this substance by humans, since a significant reduction in viability of HepG2 and a marked decrease in cell viability on normal PBMC were verified. (geneticsmr.org)
Viruses1
  • Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines . (wikipedia.org)
Journal1
Drug1
  • Curcumin, a natural polyphenolic compound derived from turmeric (Curcuma longa L), has proven to be a modulator of multiple intercellular signalling pathways linked to inflammation, to proliferation, growth, invasion, drug sensitivity, angiogenesis and metastasis of cancer cells. (researchgate.net)
Type2
  • Human wild-type Hep G2 cell line ( ab275467 ). (abcam.com)
  • Type 508 Accommodation and the title of the report in the subject line of e-mail. (cdc.gov)
Protein2
  • One of human HCC cell lines, HepG2 cells, was transfected to stably express HBx protein (HBx(+)-HepG2). (nih.gov)
  • Flow cytometry and western-blot further demonstrated that B7-H1 protein synthesis was enhanced in HBx(+)-HepG2 cells. (nih.gov)
Growth1
  • In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)
Properties2
  • But localization and trafficking properties are completely rescued for the S1426I and T1434M mutants in the differentiated cells. (bvsalud.org)
  • The localization and export properties are corrected in the differentiated cells, which is a plausible mechanism for the milder phenotype exhibited by these mutations. (bvsalud.org)
Medium1
  • Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose. (abcam.com)
Major2
  • The cells secrete a variety of major plasma proteins e.g. albumin, α2-macroglobulin, α 1-antitrypsin, transferrin and plasminogen. (sigmaaldrich.com)
  • The cells secrete a variety of major plasma proteins, e.g., albumin, and the acute-phase proteins fibrinogen, alpha 2-macroglobulin, alpha 1-antitrypsin, transferrin and plasminogen. (wikipedia.org)
Distribution1